LOC108737991 cDNA ORF clone, Agrilus planipennis(emerald ash borer)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108737991
Entrez Gene ID	108737991
Full Name	E3 ubiquitin-protein ligase TRIM33-like
Gene Type	protein-coding
Organism	Agrilus planipennis(emerald ash borer)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018471193.1	XP_018326695.1	E3 ubiquitin-protein ligase TRIM33-like
XM_018471193.2	XP_018326695.1	E3 ubiquitin-protein ligase TRIM33

Pathways from BioSystems
Gene Ontology

The following LOC108737991 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108737991 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAg21988	XM_018471193.2 Latest version!	Agrilus planipennis E3 ubiquitin-protein ligase TRIM33 (LOC108737991), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OAg21988	XM_018471193.1	Agrilus planipennis E3 ubiquitin-protein ligase TRIM33-like (LOC108737991), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108737991 ( XM_018471193.1 ) cDNA ORF clone, Agrilus planipennis(emerald ash borer) -> XP_018326695.1 Agrilus planipennis E3 ubiquitin-protein ligase TRIM33-like (LOC108737991), mRNA.

Price & Availability↑

CloneID	OAg21988
Clone ID Related Accession (Same CDS sequence)	XM_018471193.1 , XM_018471193.2
Accession Version	XM_018471193.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-21
Organism	Agrilus planipennis(emerald ash borer)
Product	E3 ubiquitin-protein ligase TRIM33-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017259758.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Agrilus planipennis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCGATTTTAA GTTGAGCGAT CAAGATTCAA TTCAAAATAA TTCAAATTTA 
CAAAATAACT CCGAATCGAA AGATTCTGAT CCTTCGGAAA ACCCGAACAT TTCAAGTGTT 
AATGTTGAAA ACATTGCTGT AATTGATAAT GCCATAGAAG AGGTTTTAAA TAGCACAAAA 
GAAGATAGCA ACCAAGATCA ATCCAATAAT TTAACCAATA GCTCCGAAGA AGAGAATGTG 
GAAAGTGGTA CATCGGGAGA CAGTTTTGTA TATAAATGTG TGTTCTGTGA ACGTATTTTA 
ACTGCTAGTG ATGAGCCTAA GTTACTGGAA TGCTTACATA ACGTTTGTGG CTCTTGTGTT 
AATAACAAAA TTTGCGAGCA AAGCGAGAGT AATATTCATG GCAGCAGTAT TCAAACAGTG 
TTAATTCCCT GTGCATCATG CAATTTACCA AGCCAAGTAG AAAGGATAAT AGAAAACCAA 
TTCTTGTTGG AGTTTTCTAA TCATGATGAT AATATTGCTA AAGCTACAGA GATAAAATGC 
AGTAGTTGTA GTGATGATGC GCTAGCAGTT AGTTGGTGTG CTGACTGTTT GGAGTATATT 
TGTGAAAGCT GTGTGCAAGC ACATCAAAGA TTGAAAATAA CGAAAGATCA TACAATTAAA 
ACCAAGGAAG AAGGCCTACT TGAGAATCAT ACATCTACAA ATGCTTCATC TAATCAAAAT 
TTAATGTGTT CTGTACATCC TCATGAGAAG TTATCTCTCT TTTGTGAAAA TTGTGATAAA 
TTGACATGTA GAGATTGCCA ACTTATAGAG CATCGAGACC ATAAATACAA ATTTACTCAT 
GAAATTGCCT CTGATACAAG AAAATTTATC TCGAATATGT TAAAGGATGT CAGTTACAAG 
CGAGTCCTAC TTGATAGTGC AATGAAAGTC ATCGCTGATC GGCAAACACT GATAAGTGAA 
AAAAAGCAAG CTATTGTTAA AGAGATCACC CAAATGGTCG TGAAATTAAC AAATACTATT 
AATATGAGAG GCAAGGAGCT TGTTTCTAAA TTAAATGAAG TTTGTGAACA GAAACAGAAA 
ACATTGCAAG AAAAGAAAGT AGCATTGGAT CAGTTATCAA AAATCACAGA TCATTGTATA 
GAATTTACTC AGCATGCATT GGAAAGTGGT AGTGATATGG CACTTTTATT TAGTAAACGT 
CAAGTAACAT GCCATTTACA AAGGATCAAG TGTAAGAGAG CAGATATTCC TAATCCTGAA 
ATTCCTGTTA GAATACATCT TGCACTTGAC AAAGTTCCAG AATTAATTAA GGTCATTTCC 
ACGATCGGGC AAATCGTGGT AGATGGCAGG GTATATCCCA TTTCACCTTC TTCGCCGGCG 
GGGTCATTAC CTCCTCATGG TTCGCCTAGC CCGGGACATA CGAGACCCCC TCAACAACAG 
TCACCCCTTA ATCAACCACC ACCTCCATCA TCTATTCAAC AACCACCACC ACAACAACCT 
CAACACTATC AACAGCAACA AATGACGTTT CAACAGCCCC GTATGTTACC ACCACCTTAC 
GGTCAACAGC ATTATCAGCA AACATCTCAA AGTATGCAGC CTGGCATGCA ACAGAATATG 
AATCAAAAAA TTCAACAAAA TATGCCTCCC CAGTTAAACA CTTTGCCAAT GACTTTGCAA 
CAGCATTTAC AACAAAGTCC CATGCAAAGA GTAACTATTC CTAGAGGTGG TATGCCAAGG 
GCAATCTTAC CAATTAACAG ACTGTCCAGT CCTGGAATCA ATCATCAACA GCAGCAGGTC 
TCATCTTCAA CACATCCGCA AGGACTTATG CAGGAACGAG GAAGTTTGAG AGGTCTTTTG 
CAACCAACAA CCAATCATAA TAGCAGCACT GTGTATGTAC AAACTGGTCC AAACCAATAT 
AGGGCTGTAC AGCCTCAGTT CGCTCAACAA TATCAAAACC GTTTTAACCA GCAGCAACAG 
GTACAACAGA TGCGCCAACA GTTGCAACAG CCAGGCGGGT CCATTCGCCT TTTGACTTCA 
AGTCATTTAC CTCCTAATAT ACAGACAAAG ATGAATGTAC AAAATCAAAA CTTCACTTCG 
CCGCCACTTC AGCAAGGGGC CAAATGGCAT ATCCCGCAAA CAGGAGATGC AGTTGCGGCA 
ACTGCTGGAG CCGGAACCTA TGCTGTTTCT GTGGTTCGCA GTCAAGCGTC CTTGCCCGTA 
AATGATAACT TCAAGATAAC GCTAAAGTCA CAAGCTAACA ACGTTGGTTC AGATACAAAA 
CCAGCTGCTG TGTCATCAAC TATACCGAAA ACACCGAGCC CCAACGGTGT ACAGGGTGGT 
AAATCGGATA CGGAACGGAA TCTCGATAAG TTCTGTCAGG ACTCAGTTAA TGATTTAATG 
GCAACGATCG CGAAACTCGA TTCGAATGGT GTTCAAGTGC TCGCGGAGGG TAATGTCAAA 
ACAACAGGTG GTTCGCCCCA CGTGGACAGT TCCACGGACG ATAGCGGCGT CGTAGGAGGT 
GTTTTGTCGG GAAAGGTGAA AGGTGACGTT GTCGATCCTG GTAAAGACGA TCCGAATGAG 
GATTGGTGCG CGGTGTGCAT GGATGGTGGC GAACTCGTCT GTTGCGATAA ATGTCCAAAG 
GTGTTTCATC AGTTCTGTCA TATACCGAAC TTAAGCGTTG AGGAAAGTGA CACATGGCAA 
TGTCTGCTCT GTACAAATAT TGCTGATTTA CCGCCTGGTG AGATTAAAGA AGGGGAACTG 
GAAACAAGAC AGCGCAAAAT ACTGGAGAGA ATAGTTCTGG AATTGTACTG TCAATATGAA 
CCAAGTCTGC CATTCAGAGA AGTGATAGGA CCAGAAAATA TAGAATATCA TTCCATTATC 
AAAAAACCAA TTGCATTGGA TACCATCAGA CACAAATTAG ACTGGAATGC CATCAACCAT 
TACACAACCA TCGATGATTT CATTAAAGAC ATTCGACTGA TGTTTAAAAA CGCTTATACA 
TATAATGACG AGAATACTCA AGTGTATCAG GATGCAAAAA CGCTAGAAAA ATTTTTCGAC 
GAACAACTTG AGAAATTTCT CCCAGAACAT GCATACGACG AATTTAGGCC TCCACCTAAA 
AAGTTTAGGA GAATAGTAAG TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018326695.1
CDS	164..3310
Translation	MAEADFKLSDQDSIQNNSNLQNNSESKDSDPSENPNISSVNVENIAVIDNAIEEVLNSTKEDSNQDQSNNLTNSSEEENVESGTSGDSFVYKCVFCERILTASDEPKLLECLHNVCGSCVNNKICEQSESNIHGSSIQTVLIPCASCNLPSQVERIIENQFLLEFSNHDDNIAKATEIKCSSCSDDALAVSWCADCLEYICESCVQAHQRLKITKDHTIKTKEEGLLENHTSTNASSNQNLMCSVHPHEKLSLFCENCDKLTCRDCQLIEHRDHKYKFTHEIASDTRKFISNMLKDVSYKRVLLDSAMKVIADRQTLISEKKQAIVKEITQMVVKLTNTINMRGKELVSKLNEVCEQKQKTLQEKKVALDQLSKITDHCIEFTQHALESGSDMALLFSKRQVTCHLQRIKCKRADIPNPEIPVRIHLALDKVPELIKVISTIGQIVVDGRVYPISPSSPAGSLPPHGSPSPGHTRPPQQQSPLNQPPPPSSIQQPPPQQPQHYQQQQMTFQQPRMLPPPYGQQHYQQTSQSMQPGMQQNMNQKIQQNMPPQLNTLPMTLQQHLQQSPMQRVTIPRGGMPRAILPINRLSSPGINHQQQQVSSSTHPQGLMQERGSLRGLLQPTTNHNSSTVYVQTGPNQYRAVQPQFAQQYQNRFNQQQQVQQMRQQLQQPGGSIRLLTSSHLPPNIQTKMNVQNQNFTSPPLQQGAKWHIPQTGDAVAATAGAGTYAVSVVRSQASLPVNDNFKITLKSQANNVGSDTKPAAVSSTIPKTPSPNGVQGGKSDTERNLDKFCQDSVNDLMATIAKLDSNGVQVLAEGNVKTTGGSPHVDSSTDDSGVVGGVLSGKVKGDVVDPGKDDPNEDWCAVCMDGGELVCCDKCPKVFHQFCHIPNLSVEESDTWQCLLCTNIADLPPGEIKEGELETRQRKILERIVLELYCQYEPSLPFREVIGPENIEYHSIIKKPIALDTIRHKLDWNAINHYTTIDDFIKDIRLMFKNAYTYNDENTQVYQDAKTLEKFFDEQLEKFLPEHAYDEFRPPPKKFRRIVSD

Target ORF information:

RefSeq Version	XM_018471193.1
Organism	Agrilus planipennis(emerald ash borer)
Definition	Agrilus planipennis E3 ubiquitin-protein ligase TRIM33-like (LOC108737991), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018471193.1

ORF Insert Sequence:

ATGGCTGAGG CCGATTTTAA GTTGAGCGAT CAAGATTCAA TTCAAAATAA TTCAAATTTA 
CAAAATAACT CCGAATCGAA AGATTCTGAT CCTTCGGAAA ACCCGAACAT TTCAAGTGTT 
AATGTTGAAA ACATTGCTGT AATTGATAAT GCCATAGAAG AGGTTTTAAA TAGCACAAAA 
GAAGATAGCA ACCAAGATCA ATCCAATAAT TTAACCAATA GCTCCGAAGA AGAGAATGTG 
GAAAGTGGTA CATCGGGAGA CAGTTTTGTA TATAAATGTG TGTTCTGTGA ACGTATTTTA 
ACTGCTAGTG ATGAGCCTAA GTTACTGGAA TGCTTACATA ACGTTTGTGG CTCTTGTGTT 
AATAACAAAA TTTGCGAGCA AAGCGAGAGT AATATTCATG GCAGCAGTAT TCAAACAGTG 
TTAATTCCCT GTGCATCATG CAATTTACCA AGCCAAGTAG AAAGGATAAT AGAAAACCAA 
TTCTTGTTGG AGTTTTCTAA TCATGATGAT AATATTGCTA AAGCTACAGA GATAAAATGC 
AGTAGTTGTA GTGATGATGC GCTAGCAGTT AGTTGGTGTG CTGACTGTTT GGAGTATATT 
TGTGAAAGCT GTGTGCAAGC ACATCAAAGA TTGAAAATAA CGAAAGATCA TACAATTAAA 
ACCAAGGAAG AAGGCCTACT TGAGAATCAT ACATCTACAA ATGCTTCATC TAATCAAAAT 
TTAATGTGTT CTGTACATCC TCATGAGAAG TTATCTCTCT TTTGTGAAAA TTGTGATAAA 
TTGACATGTA GAGATTGCCA ACTTATAGAG CATCGAGACC ATAAATACAA ATTTACTCAT 
GAAATTGCCT CTGATACAAG AAAATTTATC TCGAATATGT TAAAGGATGT CAGTTACAAG 
CGAGTCCTAC TTGATAGTGC AATGAAAGTC ATCGCTGATC GGCAAACACT GATAAGTGAA 
AAAAAGCAAG CTATTGTTAA AGAGATCACC CAAATGGTCG TGAAATTAAC AAATACTATT 
AATATGAGAG GCAAGGAGCT TGTTTCTAAA TTAAATGAAG TTTGTGAACA GAAACAGAAA 
ACATTGCAAG AAAAGAAAGT AGCATTGGAT CAGTTATCAA AAATCACAGA TCATTGTATA 
GAATTTACTC AGCATGCATT GGAAAGTGGT AGTGATATGG CACTTTTATT TAGTAAACGT 
CAAGTAACAT GCCATTTACA AAGGATCAAG TGTAAGAGAG CAGATATTCC TAATCCTGAA 
ATTCCTGTTA GAATACATCT TGCACTTGAC AAAGTTCCAG AATTAATTAA GGTCATTTCC 
ACGATCGGGC AAATCGTGGT AGATGGCAGG GTATATCCCA TTTCACCTTC TTCGCCGGCG 
GGGTCATTAC CTCCTCATGG TTCGCCTAGC CCGGGACATA CGAGACCCCC TCAACAACAG 
TCACCCCTTA ATCAACCACC ACCTCCATCA TCTATTCAAC AACCACCACC ACAACAACCT 
CAACACTATC AACAGCAACA AATGACGTTT CAACAGCCCC GTATGTTACC ACCACCTTAC 
GGTCAACAGC ATTATCAGCA AACATCTCAA AGTATGCAGC CTGGCATGCA ACAGAATATG 
AATCAAAAAA TTCAACAAAA TATGCCTCCC CAGTTAAACA CTTTGCCAAT GACTTTGCAA 
CAGCATTTAC AACAAAGTCC CATGCAAAGA GTAACTATTC CTAGAGGTGG TATGCCAAGG 
GCAATCTTAC CAATTAACAG ACTGTCCAGT CCTGGAATCA ATCATCAACA GCAGCAGGTC 
TCATCTTCAA CACATCCGCA AGGACTTATG CAGGAACGAG GAAGTTTGAG AGGTCTTTTG 
CAACCAACAA CCAATCATAA TAGCAGCACT GTGTATGTAC AAACTGGTCC AAACCAATAT 
AGGGCTGTAC AGCCTCAGTT CGCTCAACAA TATCAAAACC GTTTTAACCA GCAGCAACAG 
GTACAACAGA TGCGCCAACA GTTGCAACAG CCAGGCGGGT CCATTCGCCT TTTGACTTCA 
AGTCATTTAC CTCCTAATAT ACAGACAAAG ATGAATGTAC AAAATCAAAA CTTCACTTCG 
CCGCCACTTC AGCAAGGGGC CAAATGGCAT ATCCCGCAAA CAGGAGATGC AGTTGCGGCA 
ACTGCTGGAG CCGGAACCTA TGCTGTTTCT GTGGTTCGCA GTCAAGCGTC CTTGCCCGTA 
AATGATAACT TCAAGATAAC GCTAAAGTCA CAAGCTAACA ACGTTGGTTC AGATACAAAA 
CCAGCTGCTG TGTCATCAAC TATACCGAAA ACACCGAGCC CCAACGGTGT ACAGGGTGGT 
AAATCGGATA CGGAACGGAA TCTCGATAAG TTCTGTCAGG ACTCAGTTAA TGATTTAATG 
GCAACGATCG CGAAACTCGA TTCGAATGGT GTTCAAGTGC TCGCGGAGGG TAATGTCAAA 
ACAACAGGTG GTTCGCCCCA CGTGGACAGT TCCACGGACG ATAGCGGCGT CGTAGGAGGT 
GTTTTGTCGG GAAAGGTGAA AGGTGACGTT GTCGATCCTG GTAAAGACGA TCCGAATGAG 
GATTGGTGCG CGGTGTGCAT GGATGGTGGC GAACTCGTCT GTTGCGATAA ATGTCCAAAG 
GTGTTTCATC AGTTCTGTCA TATACCGAAC TTAAGCGTTG AGGAAAGTGA CACATGGCAA 
TGTCTGCTCT GTACAAATAT TGCTGATTTA CCGCCTGGTG AGATTAAAGA AGGGGAACTG 
GAAACAAGAC AGCGCAAAAT ACTGGAGAGA ATAGTTCTGG AATTGTACTG TCAATATGAA 
CCAAGTCTGC CATTCAGAGA AGTGATAGGA CCAGAAAATA TAGAATATCA TTCCATTATC 
AAAAAACCAA TTGCATTGGA TACCATCAGA CACAAATTAG ACTGGAATGC CATCAACCAT 
TACACAACCA TCGATGATTT CATTAAAGAC ATTCGACTGA TGTTTAAAAA CGCTTATACA 
TATAATGACG AGAATACTCA AGTGTATCAG GATGCAAAAA CGCTAGAAAA ATTTTTCGAC 
GAACAACTTG AGAAATTTCT CCCAGAACAT GCATACGACG AATTTAGGCC TCCACCTAAA 
AAGTTTAGGA GAATAGTAAG TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108737991 ( XM_018471193.2 ) cDNA ORF clone, Agrilus planipennis(emerald ash borer) -> XP_018326695.1 Agrilus planipennis E3 ubiquitin-protein ligase TRIM33 (LOC108737991), mRNA.

Price & Availability↑

CloneID	OAg21988
Clone ID Related Accession (Same CDS sequence)	XM_018471193.1 , XM_018471193.2
Accession Version	XM_018471193.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-29
Organism	Agrilus planipennis(emerald ash borer)
Product	E3 ubiquitin-protein ligase TRIM33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020438934.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 30, 2018 this sequence version replaced XM_018471193.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Agrilus planipennis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CCGATTTTAA GTTGAGCGAT CAAGATTCAA TTCAAAATAA TTCAAATTTA 
CAAAATAACT CCGAATCGAA AGATTCTGAT CCTTCGGAAA ACCCGAACAT TTCAAGTGTT 
AATGTTGAAA ACATTGCTGT AATTGATAAT GCCATAGAAG AGGTTTTAAA TAGCACAAAA 
GAAGATAGCA ACCAAGATCA ATCCAATAAT TTAACCAATA GCTCCGAAGA AGAGAATGTG 
GAAAGTGGTA CATCGGGAGA CAGTTTTGTA TATAAATGTG TGTTCTGTGA ACGTATTTTA 
ACTGCTAGTG ATGAGCCTAA GTTACTGGAA TGCTTACATA ACGTTTGTGG CTCTTGTGTT 
AATAACAAAA TTTGCGAGCA AAGCGAGAGT AATATTCATG GCAGCAGTAT TCAAACAGTG 
TTAATTCCCT GTGCATCATG CAATTTACCA AGCCAAGTAG AAAGGATAAT AGAAAACCAA 
TTCTTGTTGG AGTTTTCTAA TCATGATGAT AATATTGCTA AAGCTACAGA GATAAAATGC 
AGTAGTTGTA GTGATGATGC GCTAGCAGTT AGTTGGTGTG CTGACTGTTT GGAGTATATT 
TGTGAAAGCT GTGTGCAAGC ACATCAAAGA TTGAAAATAA CGAAAGATCA TACAATTAAA 
ACCAAGGAAG AAGGCCTACT TGAGAATCAT ACATCTACAA ATGCTTCATC TAATCAAAAT 
TTAATGTGTT CTGTACATCC TCATGAGAAG TTATCTCTCT TTTGTGAAAA TTGTGATAAA 
TTGACATGTA GAGATTGCCA ACTTATAGAG CATCGAGACC ATAAATACAA ATTTACTCAT 
GAAATTGCCT CTGATACAAG AAAATTTATC TCGAATATGT TAAAGGATGT CAGTTACAAG 
CGAGTCCTAC TTGATAGTGC AATGAAAGTC ATCGCTGATC GGCAAACACT GATAAGTGAA 
AAAAAGCAAG CTATTGTTAA AGAGATCACC CAAATGGTCG TGAAATTAAC AAATACTATT 
AATATGAGAG GCAAGGAGCT TGTTTCTAAA TTAAATGAAG TTTGTGAACA GAAACAGAAA 
ACATTGCAAG AAAAGAAAGT AGCATTGGAT CAGTTATCAA AAATCACAGA TCATTGTATA 
GAATTTACTC AGCATGCATT GGAAAGTGGT AGTGATATGG CACTTTTATT TAGTAAACGT 
CAAGTAACAT GCCATTTACA AAGGATCAAG TGTAAGAGAG CAGATATTCC TAATCCTGAA 
ATTCCTGTTA GAATACATCT TGCACTTGAC AAAGTTCCAG AATTAATTAA GGTCATTTCC 
ACGATCGGGC AAATCGTGGT AGATGGCAGG GTATATCCCA TTTCACCTTC TTCGCCGGCG 
GGGTCATTAC CTCCTCATGG TTCGCCTAGC CCGGGACATA CGAGACCCCC TCAACAACAG 
TCACCCCTTA ATCAACCACC ACCTCCATCA TCTATTCAAC AACCACCACC ACAACAACCT 
CAACACTATC AACAGCAACA AATGACGTTT CAACAGCCCC GTATGTTACC ACCACCTTAC 
GGTCAACAGC ATTATCAGCA AACATCTCAA AGTATGCAGC CTGGCATGCA ACAGAATATG 
AATCAAAAAA TTCAACAAAA TATGCCTCCC CAGTTAAACA CTTTGCCAAT GACTTTGCAA 
CAGCATTTAC AACAAAGTCC CATGCAAAGA GTAACTATTC CTAGAGGTGG TATGCCAAGG 
GCAATCTTAC CAATTAACAG ACTGTCCAGT CCTGGAATCA ATCATCAACA GCAGCAGGTC 
TCATCTTCAA CACATCCGCA AGGACTTATG CAGGAACGAG GAAGTTTGAG AGGTCTTTTG 
CAACCAACAA CCAATCATAA TAGCAGCACT GTGTATGTAC AAACTGGTCC AAACCAATAT 
AGGGCTGTAC AGCCTCAGTT CGCTCAACAA TATCAAAACC GTTTTAACCA GCAGCAACAG 
GTACAACAGA TGCGCCAACA GTTGCAACAG CCAGGCGGGT CCATTCGCCT TTTGACTTCA 
AGTCATTTAC CTCCTAATAT ACAGACAAAG ATGAATGTAC AAAATCAAAA CTTCACTTCG 
CCGCCACTTC AGCAAGGGGC CAAATGGCAT ATCCCGCAAA CAGGAGATGC AGTTGCGGCA 
ACTGCTGGAG CCGGAACCTA TGCTGTTTCT GTGGTTCGCA GTCAAGCGTC CTTGCCCGTA 
AATGATAACT TCAAGATAAC GCTAAAGTCA CAAGCTAACA ACGTTGGTTC AGATACAAAA 
CCAGCTGCTG TGTCATCAAC TATACCGAAA ACACCGAGCC CCAACGGTGT ACAGGGTGGT 
AAATCGGATA CGGAACGGAA TCTCGATAAG TTCTGTCAGG ACTCAGTTAA TGATTTAATG 
GCAACGATCG CGAAACTCGA TTCGAATGGT GTTCAAGTGC TCGCGGAGGG TAATGTCAAA 
ACAACAGGTG GTTCGCCCCA CGTGGACAGT TCCACGGACG ATAGCGGCGT CGTAGGAGGT 
GTTTTGTCGG GAAAGGTGAA AGGTGACGTT GTCGATCCTG GTAAAGACGA TCCGAATGAG 
GATTGGTGCG CGGTGTGCAT GGATGGTGGC GAACTCGTCT GTTGCGATAA ATGTCCAAAG 
GTGTTTCATC AGTTCTGTCA TATACCGAAC TTAAGCGTTG AGGAAAGTGA CACATGGCAA 
TGTCTGCTCT GTACAAATAT TGCTGATTTA CCGCCTGGTG AGATTAAAGA AGGGGAACTG 
GAAACAAGAC AGCGCAAAAT ACTGGAGAGA ATAGTTCTGG AATTGTACTG TCAATATGAA 
CCAAGTCTGC CATTCAGAGA AGTGATAGGA CCAGAAAATA TAGAATATCA TTCCATTATC 
AAAAAACCAA TTGCATTGGA TACCATCAGA CACAAATTAG ACTGGAATGC CATCAACCAT 
TACACAACCA TCGATGATTT CATTAAAGAC ATTCGACTGA TGTTTAAAAA CGCTTATACA 
TATAATGACG AGAATACTCA AGTGTATCAG GATGCAAAAA CGCTAGAAAA ATTTTTCGAC 
GAACAACTTG AGAAATTTCT CCCAGAACAT GCATACGACG AATTTAGGCC TCCACCTAAA 
AAGTTTAGGA GAATAGTAAG TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018326695.1
CDS	164..3310
Translation	MAEADFKLSDQDSIQNNSNLQNNSESKDSDPSENPNISSVNVENIAVIDNAIEEVLNSTKEDSNQDQSNNLTNSSEEENVESGTSGDSFVYKCVFCERILTASDEPKLLECLHNVCGSCVNNKICEQSESNIHGSSIQTVLIPCASCNLPSQVERIIENQFLLEFSNHDDNIAKATEIKCSSCSDDALAVSWCADCLEYICESCVQAHQRLKITKDHTIKTKEEGLLENHTSTNASSNQNLMCSVHPHEKLSLFCENCDKLTCRDCQLIEHRDHKYKFTHEIASDTRKFISNMLKDVSYKRVLLDSAMKVIADRQTLISEKKQAIVKEITQMVVKLTNTINMRGKELVSKLNEVCEQKQKTLQEKKVALDQLSKITDHCIEFTQHALESGSDMALLFSKRQVTCHLQRIKCKRADIPNPEIPVRIHLALDKVPELIKVISTIGQIVVDGRVYPISPSSPAGSLPPHGSPSPGHTRPPQQQSPLNQPPPPSSIQQPPPQQPQHYQQQQMTFQQPRMLPPPYGQQHYQQTSQSMQPGMQQNMNQKIQQNMPPQLNTLPMTLQQHLQQSPMQRVTIPRGGMPRAILPINRLSSPGINHQQQQVSSSTHPQGLMQERGSLRGLLQPTTNHNSSTVYVQTGPNQYRAVQPQFAQQYQNRFNQQQQVQQMRQQLQQPGGSIRLLTSSHLPPNIQTKMNVQNQNFTSPPLQQGAKWHIPQTGDAVAATAGAGTYAVSVVRSQASLPVNDNFKITLKSQANNVGSDTKPAAVSSTIPKTPSPNGVQGGKSDTERNLDKFCQDSVNDLMATIAKLDSNGVQVLAEGNVKTTGGSPHVDSSTDDSGVVGGVLSGKVKGDVVDPGKDDPNEDWCAVCMDGGELVCCDKCPKVFHQFCHIPNLSVEESDTWQCLLCTNIADLPPGEIKEGELETRQRKILERIVLELYCQYEPSLPFREVIGPENIEYHSIIKKPIALDTIRHKLDWNAINHYTTIDDFIKDIRLMFKNAYTYNDENTQVYQDAKTLEKFFDEQLEKFLPEHAYDEFRPPPKKFRRIVSD

Target ORF information:

RefSeq Version	XM_018471193.2
Organism	Agrilus planipennis(emerald ash borer)
Definition	Agrilus planipennis E3 ubiquitin-protein ligase TRIM33 (LOC108737991), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018471193.2

ORF Insert Sequence:

ATGGCTGAGG CCGATTTTAA GTTGAGCGAT CAAGATTCAA TTCAAAATAA TTCAAATTTA 
CAAAATAACT CCGAATCGAA AGATTCTGAT CCTTCGGAAA ACCCGAACAT TTCAAGTGTT 
AATGTTGAAA ACATTGCTGT AATTGATAAT GCCATAGAAG AGGTTTTAAA TAGCACAAAA 
GAAGATAGCA ACCAAGATCA ATCCAATAAT TTAACCAATA GCTCCGAAGA AGAGAATGTG 
GAAAGTGGTA CATCGGGAGA CAGTTTTGTA TATAAATGTG TGTTCTGTGA ACGTATTTTA 
ACTGCTAGTG ATGAGCCTAA GTTACTGGAA TGCTTACATA ACGTTTGTGG CTCTTGTGTT 
AATAACAAAA TTTGCGAGCA AAGCGAGAGT AATATTCATG GCAGCAGTAT TCAAACAGTG 
TTAATTCCCT GTGCATCATG CAATTTACCA AGCCAAGTAG AAAGGATAAT AGAAAACCAA 
TTCTTGTTGG AGTTTTCTAA TCATGATGAT AATATTGCTA AAGCTACAGA GATAAAATGC 
AGTAGTTGTA GTGATGATGC GCTAGCAGTT AGTTGGTGTG CTGACTGTTT GGAGTATATT 
TGTGAAAGCT GTGTGCAAGC ACATCAAAGA TTGAAAATAA CGAAAGATCA TACAATTAAA 
ACCAAGGAAG AAGGCCTACT TGAGAATCAT ACATCTACAA ATGCTTCATC TAATCAAAAT 
TTAATGTGTT CTGTACATCC TCATGAGAAG TTATCTCTCT TTTGTGAAAA TTGTGATAAA 
TTGACATGTA GAGATTGCCA ACTTATAGAG CATCGAGACC ATAAATACAA ATTTACTCAT 
GAAATTGCCT CTGATACAAG AAAATTTATC TCGAATATGT TAAAGGATGT CAGTTACAAG 
CGAGTCCTAC TTGATAGTGC AATGAAAGTC ATCGCTGATC GGCAAACACT GATAAGTGAA 
AAAAAGCAAG CTATTGTTAA AGAGATCACC CAAATGGTCG TGAAATTAAC AAATACTATT 
AATATGAGAG GCAAGGAGCT TGTTTCTAAA TTAAATGAAG TTTGTGAACA GAAACAGAAA 
ACATTGCAAG AAAAGAAAGT AGCATTGGAT CAGTTATCAA AAATCACAGA TCATTGTATA 
GAATTTACTC AGCATGCATT GGAAAGTGGT AGTGATATGG CACTTTTATT TAGTAAACGT 
CAAGTAACAT GCCATTTACA AAGGATCAAG TGTAAGAGAG CAGATATTCC TAATCCTGAA 
ATTCCTGTTA GAATACATCT TGCACTTGAC AAAGTTCCAG AATTAATTAA GGTCATTTCC 
ACGATCGGGC AAATCGTGGT AGATGGCAGG GTATATCCCA TTTCACCTTC TTCGCCGGCG 
GGGTCATTAC CTCCTCATGG TTCGCCTAGC CCGGGACATA CGAGACCCCC TCAACAACAG 
TCACCCCTTA ATCAACCACC ACCTCCATCA TCTATTCAAC AACCACCACC ACAACAACCT 
CAACACTATC AACAGCAACA AATGACGTTT CAACAGCCCC GTATGTTACC ACCACCTTAC 
GGTCAACAGC ATTATCAGCA AACATCTCAA AGTATGCAGC CTGGCATGCA ACAGAATATG 
AATCAAAAAA TTCAACAAAA TATGCCTCCC CAGTTAAACA CTTTGCCAAT GACTTTGCAA 
CAGCATTTAC AACAAAGTCC CATGCAAAGA GTAACTATTC CTAGAGGTGG TATGCCAAGG 
GCAATCTTAC CAATTAACAG ACTGTCCAGT CCTGGAATCA ATCATCAACA GCAGCAGGTC 
TCATCTTCAA CACATCCGCA AGGACTTATG CAGGAACGAG GAAGTTTGAG AGGTCTTTTG 
CAACCAACAA CCAATCATAA TAGCAGCACT GTGTATGTAC AAACTGGTCC AAACCAATAT 
AGGGCTGTAC AGCCTCAGTT CGCTCAACAA TATCAAAACC GTTTTAACCA GCAGCAACAG 
GTACAACAGA TGCGCCAACA GTTGCAACAG CCAGGCGGGT CCATTCGCCT TTTGACTTCA 
AGTCATTTAC CTCCTAATAT ACAGACAAAG ATGAATGTAC AAAATCAAAA CTTCACTTCG 
CCGCCACTTC AGCAAGGGGC CAAATGGCAT ATCCCGCAAA CAGGAGATGC AGTTGCGGCA 
ACTGCTGGAG CCGGAACCTA TGCTGTTTCT GTGGTTCGCA GTCAAGCGTC CTTGCCCGTA 
AATGATAACT TCAAGATAAC GCTAAAGTCA CAAGCTAACA ACGTTGGTTC AGATACAAAA 
CCAGCTGCTG TGTCATCAAC TATACCGAAA ACACCGAGCC CCAACGGTGT ACAGGGTGGT 
AAATCGGATA CGGAACGGAA TCTCGATAAG TTCTGTCAGG ACTCAGTTAA TGATTTAATG 
GCAACGATCG CGAAACTCGA TTCGAATGGT GTTCAAGTGC TCGCGGAGGG TAATGTCAAA 
ACAACAGGTG GTTCGCCCCA CGTGGACAGT TCCACGGACG ATAGCGGCGT CGTAGGAGGT 
GTTTTGTCGG GAAAGGTGAA AGGTGACGTT GTCGATCCTG GTAAAGACGA TCCGAATGAG 
GATTGGTGCG CGGTGTGCAT GGATGGTGGC GAACTCGTCT GTTGCGATAA ATGTCCAAAG 
GTGTTTCATC AGTTCTGTCA TATACCGAAC TTAAGCGTTG AGGAAAGTGA CACATGGCAA 
TGTCTGCTCT GTACAAATAT TGCTGATTTA CCGCCTGGTG AGATTAAAGA AGGGGAACTG 
GAAACAAGAC AGCGCAAAAT ACTGGAGAGA ATAGTTCTGG AATTGTACTG TCAATATGAA 
CCAAGTCTGC CATTCAGAGA AGTGATAGGA CCAGAAAATA TAGAATATCA TTCCATTATC 
AAAAAACCAA TTGCATTGGA TACCATCAGA CACAAATTAG ACTGGAATGC CATCAACCAT 
TACACAACCA TCGATGATTT CATTAAAGAC ATTCGACTGA TGTTTAAAAA CGCTTATACA 
TATAATGACG AGAATACTCA AGTGTATCAG GATGCAAAAA CGCTAGAAAA ATTTTTCGAC 
GAACAACTTG AGAAATTTCT CCCAGAACAT GCATACGACG AATTTAGGCC TCCACCTAAA 
AAGTTTAGGA GAATAGTAAG TGACTGA