LOC5574982 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC5574982
Entrez Gene ID	5574982
Full Name	uncharacterized LOC5574982
Synonyms	AAEL002498
Gene Type	protein-coding
Organism	Aedes aegypti(yellow fever mosquito)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021842044.1	XP_021697736.1	uncharacterized protein LOC5574982 isoform X2
XM_021842025.1	XP_021697717.1	uncharacterized protein LOC5574982 isoform X1
XM_001655405.1	XP_001655455.1	AAEL002498-PA
XM_021842029.1	XP_021697721.1	uncharacterized protein LOC5574982 isoform X1
XM_021842027.1	XP_021697719.1	uncharacterized protein LOC5574982 isoform X1
XM_021842032.1	XP_021697724.1	uncharacterized protein LOC5574982 isoform X1
XM_021842040.1	XP_021697732.1	uncharacterized protein LOC5574982 isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Nene V, Wortman JR, Lawson D, Haas B, Kodira C, Tu ZJ, Loftus B, Xi Z, Megy K, Grabherr M, Ren Q, Zdobnov EM, Lobo NF, Campbell KS, Brown SE, Bonaldo MF, Zhu J, Sinkins SP, Hogenkamp DG, Amedeo P, Arensburger P, Atkinson PW, Bidwell S, Biedler J, Birney E, Bruggner RV, Costas J, Coy MR, Crabtree J, Crawford M, Debruyn B, Decaprio D, Eiglmeier K, Eisenstadt E, El-Dorry H, Gelbart WM, Gomes SL, Hammond M, Hannick LI, Hogan JR, Holmes MH, Jaffe D, Johnston JS, Kennedy RC, Koo H, Kravitz S, Kriventseva EV, Kulp D, Labutti K, Lee E, Li S, Lovin DD, Mao C, Mauceli E, Menck CF, Miller JR, Montgomery P, Mori A, Nascimento AL, Naveira HF, Nusbaum C, O'leary S, Orvis J, Pertea M, Quesneville H, Reidenbach KR, Rogers YH, Roth CW, Schneider JR, Schatz M, Shumway M, Stanke M, Stinson EO, Tubio JM, Vanzee JP, Verjovski-Almeida S, Werner D, White O, Wyder S, Zeng Q, Zhao Q, Zhao Y, Hill CA, Raikhel AS, Soares MB, Knudson DL, Lee NH, Galagan J, Salzberg SL, Paulsen IT, Dimopoulos G, Collins FH, Birren B, Fraser-Liggett CM, Severson DW
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC5574982 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC5574982 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAe131559	XM_021842044.1 Latest version!	Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OAe131559	XM_021842040.1 Latest version!	Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OAe131558	XM_021842032.1 Latest version!	Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAe131558	XM_021842029.1 Latest version!	Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAe131558	XM_021842025.1 Latest version!	Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAe131558	XM_021842027.1 Latest version!	Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAe10044	XM_001655405.1 Latest version!	Aedes aegypti AAEL002498-RA partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC5574982 ( XM_021842044.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021697736.1 Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OAe131559
Clone ID Related Accession (Same CDS sequence)	XM_021842044.1 , XM_021842040.1
Accession Version	XM_021842044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-19
Organism	Aedes aegypti(yellow fever mosquito)
Product	uncharacterized protein LOC5574982 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGT ACCCAAACCG TCAACAGCAG CAGGGGAAGT TTGTGTTGTC ACAGCAGAAT 
CAGTCCCTTC TGAATCATAC TCATAACCAT ATCCATCAGC AGTTCGGTCG TCCGCTTCTC 
AGCAAGCAAA ACTCACCCAT CCGACGTCCA TTGGCTTGCA ACGCAAACTT CTGGCCCAAC 
GGCAGCAATA TCAACGCTAA CAACGCCAAC AGCAACAACA ACAACAATGC CCATACAACT 
ACGCCAACCG GAGCGTTGTC AACTGGAGTG CACGGCTTGT TTGGGGCACC CGGAATCAAC 
GGGGGCGGCG GTAGCCCAGG CCGTGACAGT CTGTTAAACG ACGAAAACCT CAAATCGAAC 
GTACAGATTG TGCTGGACAA TATCAATCGA AATTATCATG CCCTGCGGGA GCAAATACTG 
AGCAACAGTA ATCATAATAA TCTCAATAAC AACTGCAACA ACAACAACGG TAACGTAAAT 
CCTCACCACC TAGTGCTGCT GCAGCGGCTG CAGAGTGTGA GTCAGCAGTT GAAGAGGACG 
GCAGGTTTGT CGGGACAACA GCCGCAGCAG TACCAGGGCA AGATGGCGGC CAGTCCCGAT 
TCGGACACGA ACTGCAATCC GTACATGCCG CCGTTGAGCT GCTCGATCGC CTCGTCGCAG 
GATTTCAGCC ATGACTACTC GGACTATCAG TGGTTCATGG ATTACGGGAG CTATCGCGAT 
GGAATCACCC ACCAGAGCAT CCTGTCGGCG CTGTCCGCCT CGTACAACGG CATCGGCGAG 
CTGTCCTACT ACGAGGACCT CGCCAAGAAC ATCGACGCCA ACCTGGCCGA GGTGGACATG 
GAGAGCTTCC GCACCGAAGA CATCCACTCG TTGCTCACGA CGCTGCCGAC GATGTGCAGC 
GAAAGTGCCG AACTGCGTCG CAATCGGAAC AACAACCGGG GCGATCTGAT CCTGGACACG 
TGGGCCGAAG CTACGGGGAA TCATGGGGCC GGGATCACAA CGGTGGCCGA CCTGGAGAAC 
TCGATCTGCA AGTCGAAATT GCTATTCTCG CCGGTGAAGG AGTCGCACAT CAGTGTGGAC 
TCGTTGGACA TGGATGGGTA TCCGGACGAG GATGATATCA TACTGACGTG CCAGGCGAAC 
AAGAACAACT ACACGATCGC GTTCGAGCAG AGCGCGCTTT ACTCGGACGA GAGTTTCTAC 
GATGGTCCGG AGAATTATGC CAAATACAAG CATTTCAATG TGCTTCACAA CCTGGATCAC 
ATCATCAGGA AGAAGGCTCA GGAAACGGCG ATGTCCGTTT CGGAAGTGGG TTACACGACT 
TGGAGTAAGC TCAGGAAAAA TGGACCGGGA CAAAGGCTCC CAATGGTGGC GACAAATCCG 
TTACTCAACC ACATTTCCAG GCATGCACCG ACTTGTTTCG TACGAAAGAG CATGAGCATG 
CCAGATCTTA AAGGAAAGTG CGATGGAACG GAAACAGATC ATCATTCCGA AGAAGTAGAT 
CACAGCGCAG AGGCATCAGA ACACTCGCAT AAGGATCAGC AGCACAGGCA TCAGTTGAAC 
GTATCGCAAG CGGCAAGCTC CGGAGCTACT TCCGGTGAGT CGTTCGGTAA GCAGATGCGG 
ACACTACTAC CGATGTATCC GGTGCCCACC GATTCCGGCA GCGAGAGTTC CGTTCCTCTG 
AAGGAGGACT GCAACTTGGA CAATAATGAG GAGCAGCAAC AGCATCAACC GTCGTTCAAT 
TTGGTGAAGT TATTCATCAA ACAGAAAAGC AGTTCGACAG ACACCTGTAT GGATGTTTCG 
TCGGGTTGTT GGCCGAGCGA TTCTACGAAT AGTGCTTCCG GTGGTGAGAA CAACAACAAC 
AGTAACACCA ATTTGGCTGT AGCCAGTTCA AAGGATCGTT GTCGGAAGAA AAGCATGAAC 
GATTCGGGAA AATGTTCGAA CCTGGGAAGA CACGACGAGG AAGAGTTCCA GTTCGATAGT 
CTGGATGCAC AAATCAATAA CAATAACAAC AACAATGGGA CAACGCCGAT CGCCAATGGG 
GACGATAGGC AGGATCGGAC GAGGTTGAAC GATTTCAACT CCCCGGTTCA TAAGAGGAAC 
TACAAGGCAT ACAATCTGAA CATCGCCAAC AAGAATCTCC CTGCGGTGAA TCCGGTGGTG 
TACAACCTAA TGAACAACAA CAACAGTGGA GGAAGCAACG CCAGCAGCGG AACCAATCTG 
AGCAGCAACA GTATTAACAA CAATAATAAT AATAACAATA GCTTTAAGGA TACCAACTCG 
GATGCTAGTC GTACGTCGGA GAATCTGACG CAAATCTTCA ACAACAGTGG CAATAACAAT 
GGCGACGCTG CCGGTGCCAA AAGGAAGGTA CCGATCGAGA TGATTACCAA GTCGATGCAG 
ACGTCGTTCA TCGGGACCAA GGAGAAGGTC CGCGTGGTAC CTCCATCATT CCTGGAGCAC 
CTGAACAAAC TCGGTGACAA ACAGAAGGCC CCGGTCTTCG TTGTGTACCC GAACTACGTC 
CTCCCGGATT TGGGCTTCCT GAAGTCGCAA AACGATGTCG TCATTTCGCC GCTCTCGTTC 
AAAGAGAACT TCGCCCCGCG AACGCACCTT CGCAAAACGC GGCCAGTTTC GATGAACGAC 
ATCGAAAACC TCAAAGCCAA GGAATACAAG CACGTGTTGG ACTGGAAGTC GCTCATAACG 
CTGTTACCAC AGGAGTACCG AAAGCTGCTC CGTAATATTC CGGAGGCAAA TGCATGCAGC 
ATTGCGGAGG ACAATGCTCC GCAGAAGCCG CTTTTCTGTA TGACTCCGCC CTTGCGCCGG 
GGTCGAGGAG TTACCTGTGA TTGCGTGAAC ATGTTCAACA CGCAGACCTA CAATAGCTCC 
AGTTCCGGAG GAAGTTCTAG TCAGCCGCCC AGCTCGGGCT ACCGAGGTTC GTCTACGATG 
TTGACGGACT CCGAGATGGA TTTGTTGGGA CCGAGCGGTA GCGGTGGTCT TAGCAATAAC 
TTGTACGTTT ATCAGTATGA CAGCCCAGCG GAGGTGACAT CCGCGGAAAG GCCGCCTTCG 
GGACGGGTAG CACCGAAAGG TATCCTCAGG AAAACTTCAG TCCAAAGGCC TTCATCCAAG 
GCCAAGAGAA ATAGCATGTT TGAGGAGAAT CACAAATCTG GTCAGCACCT GGAAAAACGA 
CGGTCCCTGC AGGAACCGTG CTACAACTTC TCGGAAGAGA ACCTCATCTT AGAGGACTCA 
ATCAACGAAC TCCCGGACAG TGGTCTTCGC AAAATGAACA ACTCTCCAAA CATGTCCCAG 
CATCAAGCCC GTCTTCCCAA CACACCCAAA CTACCCTCGG CTGACGACTA TCATCGGTTG 
AACAAACTTA ACGAAATGAA TGATCTCGAA CTGAACAAAG CTGTCAGCAG CAAACTGAAC 
CGTGCTCAGC AACAGCAGAG CTTGGAAGAT GACTCGGAAG CCCGTACGCG TACTGAGCAT 
TTCCTGTCCA ATGTTCCAAA ATCAGAACTG AAACACTATG CCGAGCTCGC TCATTTCCTG 
GAAGCGACCA CAGCAGAAAA TGCCATAGCT CAGTCCGAGG AGGTTTACGA CCGTCAGCGG 
TTAAGGCACG AAGTCAGCCG TGCGTTATCT CAACGCAAAA ATGTGTCCTT CAACCAACCC 
CCATCCGCCG GTCCCATCAA TACCACTCCC GTCCGGCAAC AGCAAGCTCA GCTGCTGCGA 
CCGACGGAGA TCAAGTTTTC AACTCCTCCA AACTCGCCAA ACATGTCCGT TACGATGGCA 
CAGAAGCCAC CGCAGAAGCC GACTCCTTCG GCGGTGCAAT CCCCTTCAAG TGCCACCGAA 
CGGGAGAAGC AGGACAAGAT CCAATCGAAT CGTTTCAAGC GGCTGCAGAT CCAGTGGGAA 
CTGCTGAGCA AGGAGGGCCA ACAGTTGAAG CAGGAATTGC AGCAACCGCG AAGCGGTGGC 
AACACTCCTA CGGGAGGAGC AATGAAATCT CGCATTCCGC GACCCGTCAG CTATCCGACC 
ACTCGAGCAA ACTCGGATCC CATGGTGAGC AAAACGTTGA AATCGCCTAG CCGAATTGTG 
CCGCCCAAGC GGTACAATGC CGGTTCGCTG GGCACGGTAG CAGCGATCGG TGGCCACCAG 
CAGCAGGCAA CGACTCCGCC CACTCCGGCT CCCCGGACGC CCAACAAGCT ACTGCATACG 
ACTCCAAAGA AGGCCGTCAC CGCCGGTACG CCCAGGCCAG CAACACGCAC ACGGATACCA 
GTAGGTTCTG GTCAAACGCA AGGTAAATCG CTTCCCTCGT CGAATGCAGC AGTGACAGCA 
GCAAAGGTAA CGCCGAAGAA ACCCTTGAAC CACGCGGCAC CGGCTGCCAG TAGTAGTCCC 
AAGGCTACCA TCCGGAAACC GGTCAGCAGC AGTAGTGGCC GTGACGCCGA AAAACCAAAC 
AAAGGAGTCG TCAAAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021697736.1
CDS	186..4646
Translation	MSEYPNRQQQQGKFVLSQQNQSLLNHTHNHIHQQFGRPLLSKQNSPIRRPLACNANFWPNGSNINANNANSNNNNNAHTTTPTGALSTGVHGLFGAPGINGGGGSPGRDSLLNDENLKSNVQIVLDNINRNYHALREQILSNSNHNNLNNNCNNNNGNVNPHHLVLLQRLQSVSQQLKRTAGLSGQQPQQYQGKMAASPDSDTNCNPYMPPLSCSIASSQDFSHDYSDYQWFMDYGSYRDGITHQSILSALSASYNGIGELSYYEDLAKNIDANLAEVDMESFRTEDIHSLLTTLPTMCSESAELRRNRNNNRGDLILDTWAEATGNHGAGITTVADLENSICKSKLLFSPVKESHISVDSLDMDGYPDEDDIILTCQANKNNYTIAFEQSALYSDESFYDGPENYAKYKHFNVLHNLDHIIRKKAQETAMSVSEVGYTTWSKLRKNGPGQRLPMVATNPLLNHISRHAPTCFVRKSMSMPDLKGKCDGTETDHHSEEVDHSAEASEHSHKDQQHRHQLNVSQAASSGATSGESFGKQMRTLLPMYPVPTDSGSESSVPLKEDCNLDNNEEQQQHQPSFNLVKLFIKQKSSSTDTCMDVSSGCWPSDSTNSASGGENNNNSNTNLAVASSKDRCRKKSMNDSGKCSNLGRHDEEEFQFDSLDAQINNNNNNNGTTPIANGDDRQDRTRLNDFNSPVHKRNYKAYNLNIANKNLPAVNPVVYNLMNNNNSGGSNASSGTNLSSNSINNNNNNNNSFKDTNSDASRTSENLTQIFNNSGNNNGDAAGAKRKVPIEMITKSMQTSFIGTKEKVRVVPPSFLEHLNKLGDKQKAPVFVVYPNYVLPDLGFLKSQNDVVISPLSFKENFAPRTHLRKTRPVSMNDIENLKAKEYKHVLDWKSLITLLPQEYRKLLRNIPEANACSIAEDNAPQKPLFCMTPPLRRGRGVTCDCVNMFNTQTYNSSSSGGSSSQPPSSGYRGSSTMLTDSEMDLLGPSGSGGLSNNLYVYQYDSPAEVTSAERPPSGRVAPKGILRKTSVQRPSSKAKRNSMFEENHKSGQHLEKRRSLQEPCYNFSEENLILEDSINELPDSGLRKMNNSPNMSQHQARLPNTPKLPSADDYHRLNKLNEMNDLELNKAVSSKLNRAQQQQSLEDDSEARTRTEHFLSNVPKSELKHYAELAHFLEATTAENAIAQSEEVYDRQRLRHEVSRALSQRKNVSFNQPPSAGPINTTPVRQQQAQLLRPTEIKFSTPPNSPNMSVTMAQKPPQKPTPSAVQSPSSATEREKQDKIQSNRFKRLQIQWELLSKEGQQLKQELQQPRSGGNTPTGGAMKSRIPRPVSYPTTRANSDPMVSKTLKSPSRIVPPKRYNAGSLGTVAAIGGHQQQATTPPTPAPRTPNKLLHTTPKKAVTAGTPRPATRTRIPVGSGQTQGKSLPSSNAAVTAAKVTPKKPLNHAAPAASSSPKATIRKPVSSSSGRDAEKPNKGVVKV

Target ORF information:

RefSeq Version	XM_021842044.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021842044.1

ORF Insert Sequence:

ATGTCAGAGT ACCCAAACCG TCAACAGCAG CAGGGGAAGT TTGTGTTGTC ACAGCAGAAT 
CAGTCCCTTC TGAATCATAC TCATAACCAT ATCCATCAGC AGTTCGGTCG TCCGCTTCTC 
AGCAAGCAAA ACTCACCCAT CCGACGTCCA TTGGCTTGCA ACGCAAACTT CTGGCCCAAC 
GGCAGCAATA TCAACGCTAA CAACGCCAAC AGCAACAACA ACAACAATGC CCATACAACT 
ACGCCAACCG GAGCGTTGTC AACTGGAGTG CACGGCTTGT TTGGGGCACC CGGAATCAAC 
GGGGGCGGCG GTAGCCCAGG CCGTGACAGT CTGTTAAACG ACGAAAACCT CAAATCGAAC 
GTACAGATTG TGCTGGACAA TATCAATCGA AATTATCATG CCCTGCGGGA GCAAATACTG 
AGCAACAGTA ATCATAATAA TCTCAATAAC AACTGCAACA ACAACAACGG TAACGTAAAT 
CCTCACCACC TAGTGCTGCT GCAGCGGCTG CAGAGTGTGA GTCAGCAGTT GAAGAGGACG 
GCAGGTTTGT CGGGACAACA GCCGCAGCAG TACCAGGGCA AGATGGCGGC CAGTCCCGAT 
TCGGACACGA ACTGCAATCC GTACATGCCG CCGTTGAGCT GCTCGATCGC CTCGTCGCAG 
GATTTCAGCC ATGACTACTC GGACTATCAG TGGTTCATGG ATTACGGGAG CTATCGCGAT 
GGAATCACCC ACCAGAGCAT CCTGTCGGCG CTGTCCGCCT CGTACAACGG CATCGGCGAG 
CTGTCCTACT ACGAGGACCT CGCCAAGAAC ATCGACGCCA ACCTGGCCGA GGTGGACATG 
GAGAGCTTCC GCACCGAAGA CATCCACTCG TTGCTCACGA CGCTGCCGAC GATGTGCAGC 
GAAAGTGCCG AACTGCGTCG CAATCGGAAC AACAACCGGG GCGATCTGAT CCTGGACACG 
TGGGCCGAAG CTACGGGGAA TCATGGGGCC GGGATCACAA CGGTGGCCGA CCTGGAGAAC 
TCGATCTGCA AGTCGAAATT GCTATTCTCG CCGGTGAAGG AGTCGCACAT CAGTGTGGAC 
TCGTTGGACA TGGATGGGTA TCCGGACGAG GATGATATCA TACTGACGTG CCAGGCGAAC 
AAGAACAACT ACACGATCGC GTTCGAGCAG AGCGCGCTTT ACTCGGACGA GAGTTTCTAC 
GATGGTCCGG AGAATTATGC CAAATACAAG CATTTCAATG TGCTTCACAA CCTGGATCAC 
ATCATCAGGA AGAAGGCTCA GGAAACGGCG ATGTCCGTTT CGGAAGTGGG TTACACGACT 
TGGAGTAAGC TCAGGAAAAA TGGACCGGGA CAAAGGCTCC CAATGGTGGC GACAAATCCG 
TTACTCAACC ACATTTCCAG GCATGCACCG ACTTGTTTCG TACGAAAGAG CATGAGCATG 
CCAGATCTTA AAGGAAAGTG CGATGGAACG GAAACAGATC ATCATTCCGA AGAAGTAGAT 
CACAGCGCAG AGGCATCAGA ACACTCGCAT AAGGATCAGC AGCACAGGCA TCAGTTGAAC 
GTATCGCAAG CGGCAAGCTC CGGAGCTACT TCCGGTGAGT CGTTCGGTAA GCAGATGCGG 
ACACTACTAC CGATGTATCC GGTGCCCACC GATTCCGGCA GCGAGAGTTC CGTTCCTCTG 
AAGGAGGACT GCAACTTGGA CAATAATGAG GAGCAGCAAC AGCATCAACC GTCGTTCAAT 
TTGGTGAAGT TATTCATCAA ACAGAAAAGC AGTTCGACAG ACACCTGTAT GGATGTTTCG 
TCGGGTTGTT GGCCGAGCGA TTCTACGAAT AGTGCTTCCG GTGGTGAGAA CAACAACAAC 
AGTAACACCA ATTTGGCTGT AGCCAGTTCA AAGGATCGTT GTCGGAAGAA AAGCATGAAC 
GATTCGGGAA AATGTTCGAA CCTGGGAAGA CACGACGAGG AAGAGTTCCA GTTCGATAGT 
CTGGATGCAC AAATCAATAA CAATAACAAC AACAATGGGA CAACGCCGAT CGCCAATGGG 
GACGATAGGC AGGATCGGAC GAGGTTGAAC GATTTCAACT CCCCGGTTCA TAAGAGGAAC 
TACAAGGCAT ACAATCTGAA CATCGCCAAC AAGAATCTCC CTGCGGTGAA TCCGGTGGTG 
TACAACCTAA TGAACAACAA CAACAGTGGA GGAAGCAACG CCAGCAGCGG AACCAATCTG 
AGCAGCAACA GTATTAACAA CAATAATAAT AATAACAATA GCTTTAAGGA TACCAACTCG 
GATGCTAGTC GTACGTCGGA GAATCTGACG CAAATCTTCA ACAACAGTGG CAATAACAAT 
GGCGACGCTG CCGGTGCCAA AAGGAAGGTA CCGATCGAGA TGATTACCAA GTCGATGCAG 
ACGTCGTTCA TCGGGACCAA GGAGAAGGTC CGCGTGGTAC CTCCATCATT CCTGGAGCAC 
CTGAACAAAC TCGGTGACAA ACAGAAGGCC CCGGTCTTCG TTGTGTACCC GAACTACGTC 
CTCCCGGATT TGGGCTTCCT GAAGTCGCAA AACGATGTCG TCATTTCGCC GCTCTCGTTC 
AAAGAGAACT TCGCCCCGCG AACGCACCTT CGCAAAACGC GGCCAGTTTC GATGAACGAC 
ATCGAAAACC TCAAAGCCAA GGAATACAAG CACGTGTTGG ACTGGAAGTC GCTCATAACG 
CTGTTACCAC AGGAGTACCG AAAGCTGCTC CGTAATATTC CGGAGGCAAA TGCATGCAGC 
ATTGCGGAGG ACAATGCTCC GCAGAAGCCG CTTTTCTGTA TGACTCCGCC CTTGCGCCGG 
GGTCGAGGAG TTACCTGTGA TTGCGTGAAC ATGTTCAACA CGCAGACCTA CAATAGCTCC 
AGTTCCGGAG GAAGTTCTAG TCAGCCGCCC AGCTCGGGCT ACCGAGGTTC GTCTACGATG 
TTGACGGACT CCGAGATGGA TTTGTTGGGA CCGAGCGGTA GCGGTGGTCT TAGCAATAAC 
TTGTACGTTT ATCAGTATGA CAGCCCAGCG GAGGTGACAT CCGCGGAAAG GCCGCCTTCG 
GGACGGGTAG CACCGAAAGG TATCCTCAGG AAAACTTCAG TCCAAAGGCC TTCATCCAAG 
GCCAAGAGAA ATAGCATGTT TGAGGAGAAT CACAAATCTG GTCAGCACCT GGAAAAACGA 
CGGTCCCTGC AGGAACCGTG CTACAACTTC TCGGAAGAGA ACCTCATCTT AGAGGACTCA 
ATCAACGAAC TCCCGGACAG TGGTCTTCGC AAAATGAACA ACTCTCCAAA CATGTCCCAG 
CATCAAGCCC GTCTTCCCAA CACACCCAAA CTACCCTCGG CTGACGACTA TCATCGGTTG 
AACAAACTTA ACGAAATGAA TGATCTCGAA CTGAACAAAG CTGTCAGCAG CAAACTGAAC 
CGTGCTCAGC AACAGCAGAG CTTGGAAGAT GACTCGGAAG CCCGTACGCG TACTGAGCAT 
TTCCTGTCCA ATGTTCCAAA ATCAGAACTG AAACACTATG CCGAGCTCGC TCATTTCCTG 
GAAGCGACCA CAGCAGAAAA TGCCATAGCT CAGTCCGAGG AGGTTTACGA CCGTCAGCGG 
TTAAGGCACG AAGTCAGCCG TGCGTTATCT CAACGCAAAA ATGTGTCCTT CAACCAACCC 
CCATCCGCCG GTCCCATCAA TACCACTCCC GTCCGGCAAC AGCAAGCTCA GCTGCTGCGA 
CCGACGGAGA TCAAGTTTTC AACTCCTCCA AACTCGCCAA ACATGTCCGT TACGATGGCA 
CAGAAGCCAC CGCAGAAGCC GACTCCTTCG GCGGTGCAAT CCCCTTCAAG TGCCACCGAA 
CGGGAGAAGC AGGACAAGAT CCAATCGAAT CGTTTCAAGC GGCTGCAGAT CCAGTGGGAA 
CTGCTGAGCA AGGAGGGCCA ACAGTTGAAG CAGGAATTGC AGCAACCGCG AAGCGGTGGC 
AACACTCCTA CGGGAGGAGC AATGAAATCT CGCATTCCGC GACCCGTCAG CTATCCGACC 
ACTCGAGCAA ACTCGGATCC CATGGTGAGC AAAACGTTGA AATCGCCTAG CCGAATTGTG 
CCGCCCAAGC GGTACAATGC CGGTTCGCTG GGCACGGTAG CAGCGATCGG TGGCCACCAG 
CAGCAGGCAA CGACTCCGCC CACTCCGGCT CCCCGGACGC CCAACAAGCT ACTGCATACG 
ACTCCAAAGA AGGCCGTCAC CGCCGGTACG CCCAGGCCAG CAACACGCAC ACGGATACCA 
GTAGGTTCTG GTCAAACGCA AGGTAAATCG CTTCCCTCGT CGAATGCAGC AGTGACAGCA 
GCAAAGGTAA CGCCGAAGAA ACCCTTGAAC CACGCGGCAC CGGCTGCCAG TAGTAGTCCC 
AAGGCTACCA TCCGGAAACC GGTCAGCAGC AGTAGTGGCC GTGACGCCGA AAAACCAAAC 
AAAGGAGTCG TCAAAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5574982 ( XM_021842040.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021697732.1 Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OAe131559
Clone ID Related Accession (Same CDS sequence)	XM_021842044.1 , XM_021842040.1
Accession Version	XM_021842040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-19
Organism	Aedes aegypti(yellow fever mosquito)
Product	uncharacterized protein LOC5574982 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGT ACCCAAACCG TCAACAGCAG CAGGGGAAGT TTGTGTTGTC ACAGCAGAAT 
CAGTCCCTTC TGAATCATAC TCATAACCAT ATCCATCAGC AGTTCGGTCG TCCGCTTCTC 
AGCAAGCAAA ACTCACCCAT CCGACGTCCA TTGGCTTGCA ACGCAAACTT CTGGCCCAAC 
GGCAGCAATA TCAACGCTAA CAACGCCAAC AGCAACAACA ACAACAATGC CCATACAACT 
ACGCCAACCG GAGCGTTGTC AACTGGAGTG CACGGCTTGT TTGGGGCACC CGGAATCAAC 
GGGGGCGGCG GTAGCCCAGG CCGTGACAGT CTGTTAAACG ACGAAAACCT CAAATCGAAC 
GTACAGATTG TGCTGGACAA TATCAATCGA AATTATCATG CCCTGCGGGA GCAAATACTG 
AGCAACAGTA ATCATAATAA TCTCAATAAC AACTGCAACA ACAACAACGG TAACGTAAAT 
CCTCACCACC TAGTGCTGCT GCAGCGGCTG CAGAGTGTGA GTCAGCAGTT GAAGAGGACG 
GCAGGTTTGT CGGGACAACA GCCGCAGCAG TACCAGGGCA AGATGGCGGC CAGTCCCGAT 
TCGGACACGA ACTGCAATCC GTACATGCCG CCGTTGAGCT GCTCGATCGC CTCGTCGCAG 
GATTTCAGCC ATGACTACTC GGACTATCAG TGGTTCATGG ATTACGGGAG CTATCGCGAT 
GGAATCACCC ACCAGAGCAT CCTGTCGGCG CTGTCCGCCT CGTACAACGG CATCGGCGAG 
CTGTCCTACT ACGAGGACCT CGCCAAGAAC ATCGACGCCA ACCTGGCCGA GGTGGACATG 
GAGAGCTTCC GCACCGAAGA CATCCACTCG TTGCTCACGA CGCTGCCGAC GATGTGCAGC 
GAAAGTGCCG AACTGCGTCG CAATCGGAAC AACAACCGGG GCGATCTGAT CCTGGACACG 
TGGGCCGAAG CTACGGGGAA TCATGGGGCC GGGATCACAA CGGTGGCCGA CCTGGAGAAC 
TCGATCTGCA AGTCGAAATT GCTATTCTCG CCGGTGAAGG AGTCGCACAT CAGTGTGGAC 
TCGTTGGACA TGGATGGGTA TCCGGACGAG GATGATATCA TACTGACGTG CCAGGCGAAC 
AAGAACAACT ACACGATCGC GTTCGAGCAG AGCGCGCTTT ACTCGGACGA GAGTTTCTAC 
GATGGTCCGG AGAATTATGC CAAATACAAG CATTTCAATG TGCTTCACAA CCTGGATCAC 
ATCATCAGGA AGAAGGCTCA GGAAACGGCG ATGTCCGTTT CGGAAGTGGG TTACACGACT 
TGGAGTAAGC TCAGGAAAAA TGGACCGGGA CAAAGGCTCC CAATGGTGGC GACAAATCCG 
TTACTCAACC ACATTTCCAG GCATGCACCG ACTTGTTTCG TACGAAAGAG CATGAGCATG 
CCAGATCTTA AAGGAAAGTG CGATGGAACG GAAACAGATC ATCATTCCGA AGAAGTAGAT 
CACAGCGCAG AGGCATCAGA ACACTCGCAT AAGGATCAGC AGCACAGGCA TCAGTTGAAC 
GTATCGCAAG CGGCAAGCTC CGGAGCTACT TCCGGTGAGT CGTTCGGTAA GCAGATGCGG 
ACACTACTAC CGATGTATCC GGTGCCCACC GATTCCGGCA GCGAGAGTTC CGTTCCTCTG 
AAGGAGGACT GCAACTTGGA CAATAATGAG GAGCAGCAAC AGCATCAACC GTCGTTCAAT 
TTGGTGAAGT TATTCATCAA ACAGAAAAGC AGTTCGACAG ACACCTGTAT GGATGTTTCG 
TCGGGTTGTT GGCCGAGCGA TTCTACGAAT AGTGCTTCCG GTGGTGAGAA CAACAACAAC 
AGTAACACCA ATTTGGCTGT AGCCAGTTCA AAGGATCGTT GTCGGAAGAA AAGCATGAAC 
GATTCGGGAA AATGTTCGAA CCTGGGAAGA CACGACGAGG AAGAGTTCCA GTTCGATAGT 
CTGGATGCAC AAATCAATAA CAATAACAAC AACAATGGGA CAACGCCGAT CGCCAATGGG 
GACGATAGGC AGGATCGGAC GAGGTTGAAC GATTTCAACT CCCCGGTTCA TAAGAGGAAC 
TACAAGGCAT ACAATCTGAA CATCGCCAAC AAGAATCTCC CTGCGGTGAA TCCGGTGGTG 
TACAACCTAA TGAACAACAA CAACAGTGGA GGAAGCAACG CCAGCAGCGG AACCAATCTG 
AGCAGCAACA GTATTAACAA CAATAATAAT AATAACAATA GCTTTAAGGA TACCAACTCG 
GATGCTAGTC GTACGTCGGA GAATCTGACG CAAATCTTCA ACAACAGTGG CAATAACAAT 
GGCGACGCTG CCGGTGCCAA AAGGAAGGTA CCGATCGAGA TGATTACCAA GTCGATGCAG 
ACGTCGTTCA TCGGGACCAA GGAGAAGGTC CGCGTGGTAC CTCCATCATT CCTGGAGCAC 
CTGAACAAAC TCGGTGACAA ACAGAAGGCC CCGGTCTTCG TTGTGTACCC GAACTACGTC 
CTCCCGGATT TGGGCTTCCT GAAGTCGCAA AACGATGTCG TCATTTCGCC GCTCTCGTTC 
AAAGAGAACT TCGCCCCGCG AACGCACCTT CGCAAAACGC GGCCAGTTTC GATGAACGAC 
ATCGAAAACC TCAAAGCCAA GGAATACAAG CACGTGTTGG ACTGGAAGTC GCTCATAACG 
CTGTTACCAC AGGAGTACCG AAAGCTGCTC CGTAATATTC CGGAGGCAAA TGCATGCAGC 
ATTGCGGAGG ACAATGCTCC GCAGAAGCCG CTTTTCTGTA TGACTCCGCC CTTGCGCCGG 
GGTCGAGGAG TTACCTGTGA TTGCGTGAAC ATGTTCAACA CGCAGACCTA CAATAGCTCC 
AGTTCCGGAG GAAGTTCTAG TCAGCCGCCC AGCTCGGGCT ACCGAGGTTC GTCTACGATG 
TTGACGGACT CCGAGATGGA TTTGTTGGGA CCGAGCGGTA GCGGTGGTCT TAGCAATAAC 
TTGTACGTTT ATCAGTATGA CAGCCCAGCG GAGGTGACAT CCGCGGAAAG GCCGCCTTCG 
GGACGGGTAG CACCGAAAGG TATCCTCAGG AAAACTTCAG TCCAAAGGCC TTCATCCAAG 
GCCAAGAGAA ATAGCATGTT TGAGGAGAAT CACAAATCTG GTCAGCACCT GGAAAAACGA 
CGGTCCCTGC AGGAACCGTG CTACAACTTC TCGGAAGAGA ACCTCATCTT AGAGGACTCA 
ATCAACGAAC TCCCGGACAG TGGTCTTCGC AAAATGAACA ACTCTCCAAA CATGTCCCAG 
CATCAAGCCC GTCTTCCCAA CACACCCAAA CTACCCTCGG CTGACGACTA TCATCGGTTG 
AACAAACTTA ACGAAATGAA TGATCTCGAA CTGAACAAAG CTGTCAGCAG CAAACTGAAC 
CGTGCTCAGC AACAGCAGAG CTTGGAAGAT GACTCGGAAG CCCGTACGCG TACTGAGCAT 
TTCCTGTCCA ATGTTCCAAA ATCAGAACTG AAACACTATG CCGAGCTCGC TCATTTCCTG 
GAAGCGACCA CAGCAGAAAA TGCCATAGCT CAGTCCGAGG AGGTTTACGA CCGTCAGCGG 
TTAAGGCACG AAGTCAGCCG TGCGTTATCT CAACGCAAAA ATGTGTCCTT CAACCAACCC 
CCATCCGCCG GTCCCATCAA TACCACTCCC GTCCGGCAAC AGCAAGCTCA GCTGCTGCGA 
CCGACGGAGA TCAAGTTTTC AACTCCTCCA AACTCGCCAA ACATGTCCGT TACGATGGCA 
CAGAAGCCAC CGCAGAAGCC GACTCCTTCG GCGGTGCAAT CCCCTTCAAG TGCCACCGAA 
CGGGAGAAGC AGGACAAGAT CCAATCGAAT CGTTTCAAGC GGCTGCAGAT CCAGTGGGAA 
CTGCTGAGCA AGGAGGGCCA ACAGTTGAAG CAGGAATTGC AGCAACCGCG AAGCGGTGGC 
AACACTCCTA CGGGAGGAGC AATGAAATCT CGCATTCCGC GACCCGTCAG CTATCCGACC 
ACTCGAGCAA ACTCGGATCC CATGGTGAGC AAAACGTTGA AATCGCCTAG CCGAATTGTG 
CCGCCCAAGC GGTACAATGC CGGTTCGCTG GGCACGGTAG CAGCGATCGG TGGCCACCAG 
CAGCAGGCAA CGACTCCGCC CACTCCGGCT CCCCGGACGC CCAACAAGCT ACTGCATACG 
ACTCCAAAGA AGGCCGTCAC CGCCGGTACG CCCAGGCCAG CAACACGCAC ACGGATACCA 
GTAGGTTCTG GTCAAACGCA AGGTAAATCG CTTCCCTCGT CGAATGCAGC AGTGACAGCA 
GCAAAGGTAA CGCCGAAGAA ACCCTTGAAC CACGCGGCAC CGGCTGCCAG TAGTAGTCCC 
AAGGCTACCA TCCGGAAACC GGTCAGCAGC AGTAGTGGCC GTGACGCCGA AAAACCAAAC 
AAAGGAGTCG TCAAAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021697732.1
CDS	965..5425
Translation	MSEYPNRQQQQGKFVLSQQNQSLLNHTHNHIHQQFGRPLLSKQNSPIRRPLACNANFWPNGSNINANNANSNNNNNAHTTTPTGALSTGVHGLFGAPGINGGGGSPGRDSLLNDENLKSNVQIVLDNINRNYHALREQILSNSNHNNLNNNCNNNNGNVNPHHLVLLQRLQSVSQQLKRTAGLSGQQPQQYQGKMAASPDSDTNCNPYMPPLSCSIASSQDFSHDYSDYQWFMDYGSYRDGITHQSILSALSASYNGIGELSYYEDLAKNIDANLAEVDMESFRTEDIHSLLTTLPTMCSESAELRRNRNNNRGDLILDTWAEATGNHGAGITTVADLENSICKSKLLFSPVKESHISVDSLDMDGYPDEDDIILTCQANKNNYTIAFEQSALYSDESFYDGPENYAKYKHFNVLHNLDHIIRKKAQETAMSVSEVGYTTWSKLRKNGPGQRLPMVATNPLLNHISRHAPTCFVRKSMSMPDLKGKCDGTETDHHSEEVDHSAEASEHSHKDQQHRHQLNVSQAASSGATSGESFGKQMRTLLPMYPVPTDSGSESSVPLKEDCNLDNNEEQQQHQPSFNLVKLFIKQKSSSTDTCMDVSSGCWPSDSTNSASGGENNNNSNTNLAVASSKDRCRKKSMNDSGKCSNLGRHDEEEFQFDSLDAQINNNNNNNGTTPIANGDDRQDRTRLNDFNSPVHKRNYKAYNLNIANKNLPAVNPVVYNLMNNNNSGGSNASSGTNLSSNSINNNNNNNNSFKDTNSDASRTSENLTQIFNNSGNNNGDAAGAKRKVPIEMITKSMQTSFIGTKEKVRVVPPSFLEHLNKLGDKQKAPVFVVYPNYVLPDLGFLKSQNDVVISPLSFKENFAPRTHLRKTRPVSMNDIENLKAKEYKHVLDWKSLITLLPQEYRKLLRNIPEANACSIAEDNAPQKPLFCMTPPLRRGRGVTCDCVNMFNTQTYNSSSSGGSSSQPPSSGYRGSSTMLTDSEMDLLGPSGSGGLSNNLYVYQYDSPAEVTSAERPPSGRVAPKGILRKTSVQRPSSKAKRNSMFEENHKSGQHLEKRRSLQEPCYNFSEENLILEDSINELPDSGLRKMNNSPNMSQHQARLPNTPKLPSADDYHRLNKLNEMNDLELNKAVSSKLNRAQQQQSLEDDSEARTRTEHFLSNVPKSELKHYAELAHFLEATTAENAIAQSEEVYDRQRLRHEVSRALSQRKNVSFNQPPSAGPINTTPVRQQQAQLLRPTEIKFSTPPNSPNMSVTMAQKPPQKPTPSAVQSPSSATEREKQDKIQSNRFKRLQIQWELLSKEGQQLKQELQQPRSGGNTPTGGAMKSRIPRPVSYPTTRANSDPMVSKTLKSPSRIVPPKRYNAGSLGTVAAIGGHQQQATTPPTPAPRTPNKLLHTTPKKAVTAGTPRPATRTRIPVGSGQTQGKSLPSSNAAVTAAKVTPKKPLNHAAPAASSSPKATIRKPVSSSSGRDAEKPNKGVVKV

Target ORF information:

RefSeq Version	XM_021842040.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021842040.1

ORF Insert Sequence:

ATGTCAGAGT ACCCAAACCG TCAACAGCAG CAGGGGAAGT TTGTGTTGTC ACAGCAGAAT 
CAGTCCCTTC TGAATCATAC TCATAACCAT ATCCATCAGC AGTTCGGTCG TCCGCTTCTC 
AGCAAGCAAA ACTCACCCAT CCGACGTCCA TTGGCTTGCA ACGCAAACTT CTGGCCCAAC 
GGCAGCAATA TCAACGCTAA CAACGCCAAC AGCAACAACA ACAACAATGC CCATACAACT 
ACGCCAACCG GAGCGTTGTC AACTGGAGTG CACGGCTTGT TTGGGGCACC CGGAATCAAC 
GGGGGCGGCG GTAGCCCAGG CCGTGACAGT CTGTTAAACG ACGAAAACCT CAAATCGAAC 
GTACAGATTG TGCTGGACAA TATCAATCGA AATTATCATG CCCTGCGGGA GCAAATACTG 
AGCAACAGTA ATCATAATAA TCTCAATAAC AACTGCAACA ACAACAACGG TAACGTAAAT 
CCTCACCACC TAGTGCTGCT GCAGCGGCTG CAGAGTGTGA GTCAGCAGTT GAAGAGGACG 
GCAGGTTTGT CGGGACAACA GCCGCAGCAG TACCAGGGCA AGATGGCGGC CAGTCCCGAT 
TCGGACACGA ACTGCAATCC GTACATGCCG CCGTTGAGCT GCTCGATCGC CTCGTCGCAG 
GATTTCAGCC ATGACTACTC GGACTATCAG TGGTTCATGG ATTACGGGAG CTATCGCGAT 
GGAATCACCC ACCAGAGCAT CCTGTCGGCG CTGTCCGCCT CGTACAACGG CATCGGCGAG 
CTGTCCTACT ACGAGGACCT CGCCAAGAAC ATCGACGCCA ACCTGGCCGA GGTGGACATG 
GAGAGCTTCC GCACCGAAGA CATCCACTCG TTGCTCACGA CGCTGCCGAC GATGTGCAGC 
GAAAGTGCCG AACTGCGTCG CAATCGGAAC AACAACCGGG GCGATCTGAT CCTGGACACG 
TGGGCCGAAG CTACGGGGAA TCATGGGGCC GGGATCACAA CGGTGGCCGA CCTGGAGAAC 
TCGATCTGCA AGTCGAAATT GCTATTCTCG CCGGTGAAGG AGTCGCACAT CAGTGTGGAC 
TCGTTGGACA TGGATGGGTA TCCGGACGAG GATGATATCA TACTGACGTG CCAGGCGAAC 
AAGAACAACT ACACGATCGC GTTCGAGCAG AGCGCGCTTT ACTCGGACGA GAGTTTCTAC 
GATGGTCCGG AGAATTATGC CAAATACAAG CATTTCAATG TGCTTCACAA CCTGGATCAC 
ATCATCAGGA AGAAGGCTCA GGAAACGGCG ATGTCCGTTT CGGAAGTGGG TTACACGACT 
TGGAGTAAGC TCAGGAAAAA TGGACCGGGA CAAAGGCTCC CAATGGTGGC GACAAATCCG 
TTACTCAACC ACATTTCCAG GCATGCACCG ACTTGTTTCG TACGAAAGAG CATGAGCATG 
CCAGATCTTA AAGGAAAGTG CGATGGAACG GAAACAGATC ATCATTCCGA AGAAGTAGAT 
CACAGCGCAG AGGCATCAGA ACACTCGCAT AAGGATCAGC AGCACAGGCA TCAGTTGAAC 
GTATCGCAAG CGGCAAGCTC CGGAGCTACT TCCGGTGAGT CGTTCGGTAA GCAGATGCGG 
ACACTACTAC CGATGTATCC GGTGCCCACC GATTCCGGCA GCGAGAGTTC CGTTCCTCTG 
AAGGAGGACT GCAACTTGGA CAATAATGAG GAGCAGCAAC AGCATCAACC GTCGTTCAAT 
TTGGTGAAGT TATTCATCAA ACAGAAAAGC AGTTCGACAG ACACCTGTAT GGATGTTTCG 
TCGGGTTGTT GGCCGAGCGA TTCTACGAAT AGTGCTTCCG GTGGTGAGAA CAACAACAAC 
AGTAACACCA ATTTGGCTGT AGCCAGTTCA AAGGATCGTT GTCGGAAGAA AAGCATGAAC 
GATTCGGGAA AATGTTCGAA CCTGGGAAGA CACGACGAGG AAGAGTTCCA GTTCGATAGT 
CTGGATGCAC AAATCAATAA CAATAACAAC AACAATGGGA CAACGCCGAT CGCCAATGGG 
GACGATAGGC AGGATCGGAC GAGGTTGAAC GATTTCAACT CCCCGGTTCA TAAGAGGAAC 
TACAAGGCAT ACAATCTGAA CATCGCCAAC AAGAATCTCC CTGCGGTGAA TCCGGTGGTG 
TACAACCTAA TGAACAACAA CAACAGTGGA GGAAGCAACG CCAGCAGCGG AACCAATCTG 
AGCAGCAACA GTATTAACAA CAATAATAAT AATAACAATA GCTTTAAGGA TACCAACTCG 
GATGCTAGTC GTACGTCGGA GAATCTGACG CAAATCTTCA ACAACAGTGG CAATAACAAT 
GGCGACGCTG CCGGTGCCAA AAGGAAGGTA CCGATCGAGA TGATTACCAA GTCGATGCAG 
ACGTCGTTCA TCGGGACCAA GGAGAAGGTC CGCGTGGTAC CTCCATCATT CCTGGAGCAC 
CTGAACAAAC TCGGTGACAA ACAGAAGGCC CCGGTCTTCG TTGTGTACCC GAACTACGTC 
CTCCCGGATT TGGGCTTCCT GAAGTCGCAA AACGATGTCG TCATTTCGCC GCTCTCGTTC 
AAAGAGAACT TCGCCCCGCG AACGCACCTT CGCAAAACGC GGCCAGTTTC GATGAACGAC 
ATCGAAAACC TCAAAGCCAA GGAATACAAG CACGTGTTGG ACTGGAAGTC GCTCATAACG 
CTGTTACCAC AGGAGTACCG AAAGCTGCTC CGTAATATTC CGGAGGCAAA TGCATGCAGC 
ATTGCGGAGG ACAATGCTCC GCAGAAGCCG CTTTTCTGTA TGACTCCGCC CTTGCGCCGG 
GGTCGAGGAG TTACCTGTGA TTGCGTGAAC ATGTTCAACA CGCAGACCTA CAATAGCTCC 
AGTTCCGGAG GAAGTTCTAG TCAGCCGCCC AGCTCGGGCT ACCGAGGTTC GTCTACGATG 
TTGACGGACT CCGAGATGGA TTTGTTGGGA CCGAGCGGTA GCGGTGGTCT TAGCAATAAC 
TTGTACGTTT ATCAGTATGA CAGCCCAGCG GAGGTGACAT CCGCGGAAAG GCCGCCTTCG 
GGACGGGTAG CACCGAAAGG TATCCTCAGG AAAACTTCAG TCCAAAGGCC TTCATCCAAG 
GCCAAGAGAA ATAGCATGTT TGAGGAGAAT CACAAATCTG GTCAGCACCT GGAAAAACGA 
CGGTCCCTGC AGGAACCGTG CTACAACTTC TCGGAAGAGA ACCTCATCTT AGAGGACTCA 
ATCAACGAAC TCCCGGACAG TGGTCTTCGC AAAATGAACA ACTCTCCAAA CATGTCCCAG 
CATCAAGCCC GTCTTCCCAA CACACCCAAA CTACCCTCGG CTGACGACTA TCATCGGTTG 
AACAAACTTA ACGAAATGAA TGATCTCGAA CTGAACAAAG CTGTCAGCAG CAAACTGAAC 
CGTGCTCAGC AACAGCAGAG CTTGGAAGAT GACTCGGAAG CCCGTACGCG TACTGAGCAT 
TTCCTGTCCA ATGTTCCAAA ATCAGAACTG AAACACTATG CCGAGCTCGC TCATTTCCTG 
GAAGCGACCA CAGCAGAAAA TGCCATAGCT CAGTCCGAGG AGGTTTACGA CCGTCAGCGG 
TTAAGGCACG AAGTCAGCCG TGCGTTATCT CAACGCAAAA ATGTGTCCTT CAACCAACCC 
CCATCCGCCG GTCCCATCAA TACCACTCCC GTCCGGCAAC AGCAAGCTCA GCTGCTGCGA 
CCGACGGAGA TCAAGTTTTC AACTCCTCCA AACTCGCCAA ACATGTCCGT TACGATGGCA 
CAGAAGCCAC CGCAGAAGCC GACTCCTTCG GCGGTGCAAT CCCCTTCAAG TGCCACCGAA 
CGGGAGAAGC AGGACAAGAT CCAATCGAAT CGTTTCAAGC GGCTGCAGAT CCAGTGGGAA 
CTGCTGAGCA AGGAGGGCCA ACAGTTGAAG CAGGAATTGC AGCAACCGCG AAGCGGTGGC 
AACACTCCTA CGGGAGGAGC AATGAAATCT CGCATTCCGC GACCCGTCAG CTATCCGACC 
ACTCGAGCAA ACTCGGATCC CATGGTGAGC AAAACGTTGA AATCGCCTAG CCGAATTGTG 
CCGCCCAAGC GGTACAATGC CGGTTCGCTG GGCACGGTAG CAGCGATCGG TGGCCACCAG 
CAGCAGGCAA CGACTCCGCC CACTCCGGCT CCCCGGACGC CCAACAAGCT ACTGCATACG 
ACTCCAAAGA AGGCCGTCAC CGCCGGTACG CCCAGGCCAG CAACACGCAC ACGGATACCA 
GTAGGTTCTG GTCAAACGCA AGGTAAATCG CTTCCCTCGT CGAATGCAGC AGTGACAGCA 
GCAAAGGTAA CGCCGAAGAA ACCCTTGAAC CACGCGGCAC CGGCTGCCAG TAGTAGTCCC 
AAGGCTACCA TCCGGAAACC GGTCAGCAGC AGTAGTGGCC GTGACGCCGA AAAACCAAAC 
AAAGGAGTCG TCAAAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5574982 ( XM_021842025.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021697717.1 Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X1, mRNA.

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CloneID	OAe131558
Clone ID Related Accession (Same CDS sequence)	XM_021842025.1 , XM_021842029.1 , XM_021842027.1 , XM_021842032.1
Accession Version	XM_021842025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-19
Organism	Aedes aegypti(yellow fever mosquito)
Product	uncharacterized protein LOC5574982 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGT GTTTGTTTGT GTTTCTGTGG AAAATCCTAC GAAATATGTC CACCATATCG 
GCAACGATAC AGCAAGAGCA GCAACTGGAA CTGCTCCGGC AGCAGTTGGT AGAACATCAG 
CGAAGAAAGT TAAGACAGTT GCAACAGCAG AAGCCGGGCA GTGGCAATTA TCAAAAACTT 
CGAAAACTGT GTCGTGATAA GGCGGCAGCT ACCAAAGAAA ACTTTCTGCT GCACAAATTT 
TTGTTCGGGT CCGAGGCGAT CCCGGAGAAC GGAACGGTGA ATGGTGATAA GGGCGGTGAT 
GGTAGCTGTG ATAAAATGGA TTACTTGAGT GATAAAGATC TTAAATTTCT TCGTATGTCA 
GAGTACCCAA ACCGTCAACA GCAGCAGGGG AAGTTTGTGT TGTCACAGCA GAATCAGTCC 
CTTCTGAATC ATACTCATAA CCATATCCAT CAGCAGTTCG GTCGTCCGCT TCTCAGCAAG 
CAAAACTCAC CCATCCGACG TCCATTGGCT TGCAACGCAA ACTTCTGGCC CAACGGCAGC 
AATATCAACG CTAACAACGC CAACAGCAAC AACAACAACA ATGCCCATAC AACTACGCCA 
ACCGGAGCGT TGTCAACTGG AGTGCACGGC TTGTTTGGGG CACCCGGAAT CAACGGGGGC 
GGCGGTAGCC CAGGCCGTGA CAGTCTGTTA AACGACGAAA ACCTCAAATC GAACGTACAG 
ATTGTGCTGG ACAATATCAA TCGAAATTAT CATGCCCTGC GGGAGCAAAT ACTGAGCAAC 
AGTAATCATA ATAATCTCAA TAACAACTGC AACAACAACA ACGGTAACGT AAATCCTCAC 
CACCTAGTGC TGCTGCAGCG GCTGCAGAGT GTGAGTCAGC AGTTGAAGAG GACGGCAGGT 
TTGTCGGGAC AACAGCCGCA GCAGTACCAG GGCAAGATGG CGGCCAGTCC CGATTCGGAC 
ACGAACTGCA ATCCGTACAT GCCGCCGTTG AGCTGCTCGA TCGCCTCGTC GCAGGATTTC 
AGCCATGACT ACTCGGACTA TCAGTGGTTC ATGGATTACG GGAGCTATCG CGATGGAATC 
ACCCACCAGA GCATCCTGTC GGCGCTGTCC GCCTCGTACA ACGGCATCGG CGAGCTGTCC 
TACTACGAGG ACCTCGCCAA GAACATCGAC GCCAACCTGG CCGAGGTGGA CATGGAGAGC 
TTCCGCACCG AAGACATCCA CTCGTTGCTC ACGACGCTGC CGACGATGTG CAGCGAAAGT 
GCCGAACTGC GTCGCAATCG GAACAACAAC CGGGGCGATC TGATCCTGGA CACGTGGGCC 
GAAGCTACGG GGAATCATGG GGCCGGGATC ACAACGGTGG CCGACCTGGA GAACTCGATC 
TGCAAGTCGA AATTGCTATT CTCGCCGGTG AAGGAGTCGC ACATCAGTGT GGACTCGTTG 
GACATGGATG GGTATCCGGA CGAGGATGAT ATCATACTGA CGTGCCAGGC GAACAAGAAC 
AACTACACGA TCGCGTTCGA GCAGAGCGCG CTTTACTCGG ACGAGAGTTT CTACGATGGT 
CCGGAGAATT ATGCCAAATA CAAGCATTTC AATGTGCTTC ACAACCTGGA TCACATCATC 
AGGAAGAAGG CTCAGGAAAC GGCGATGTCC GTTTCGGAAG TGGGTTACAC GACTTGGAGT 
AAGCTCAGGA AAAATGGACC GGGACAAAGG CTCCCAATGG TGGCGACAAA TCCGTTACTC 
AACCACATTT CCAGGCATGC ACCGACTTGT TTCGTACGAA AGAGCATGAG CATGCCAGAT 
CTTAAAGGAA AGTGCGATGG AACGGAAACA GATCATCATT CCGAAGAAGT AGATCACAGC 
GCAGAGGCAT CAGAACACTC GCATAAGGAT CAGCAGCACA GGCATCAGTT GAACGTATCG 
CAAGCGGCAA GCTCCGGAGC TACTTCCGGT GAGTCGTTCG GTAAGCAGAT GCGGACACTA 
CTACCGATGT ATCCGGTGCC CACCGATTCC GGCAGCGAGA GTTCCGTTCC TCTGAAGGAG 
GACTGCAACT TGGACAATAA TGAGGAGCAG CAACAGCATC AACCGTCGTT CAATTTGGTG 
AAGTTATTCA TCAAACAGAA AAGCAGTTCG ACAGACACCT GTATGGATGT TTCGTCGGGT 
TGTTGGCCGA GCGATTCTAC GAATAGTGCT TCCGGTGGTG AGAACAACAA CAACAGTAAC 
ACCAATTTGG CTGTAGCCAG TTCAAAGGAT CGTTGTCGGA AGAAAAGCAT GAACGATTCG 
GGAAAATGTT CGAACCTGGG AAGACACGAC GAGGAAGAGT TCCAGTTCGA TAGTCTGGAT 
GCACAAATCA ATAACAATAA CAACAACAAT GGGACAACGC CGATCGCCAA TGGGGACGAT 
AGGCAGGATC GGACGAGGTT GAACGATTTC AACTCCCCGG TTCATAAGAG GAACTACAAG 
GCATACAATC TGAACATCGC CAACAAGAAT CTCCCTGCGG TGAATCCGGT GGTGTACAAC 
CTAATGAACA ACAACAACAG TGGAGGAAGC AACGCCAGCA GCGGAACCAA TCTGAGCAGC 
AACAGTATTA ACAACAATAA TAATAATAAC AATAGCTTTA AGGATACCAA CTCGGATGCT 
AGTCGTACGT CGGAGAATCT GACGCAAATC TTCAACAACA GTGGCAATAA CAATGGCGAC 
GCTGCCGGTG CCAAAAGGAA GGTACCGATC GAGATGATTA CCAAGTCGAT GCAGACGTCG 
TTCATCGGGA CCAAGGAGAA GGTCCGCGTG GTACCTCCAT CATTCCTGGA GCACCTGAAC 
AAACTCGGTG ACAAACAGAA GGCCCCGGTC TTCGTTGTGT ACCCGAACTA CGTCCTCCCG 
GATTTGGGCT TCCTGAAGTC GCAAAACGAT GTCGTCATTT CGCCGCTCTC GTTCAAAGAG 
AACTTCGCCC CGCGAACGCA CCTTCGCAAA ACGCGGCCAG TTTCGATGAA CGACATCGAA 
AACCTCAAAG CCAAGGAATA CAAGCACGTG TTGGACTGGA AGTCGCTCAT AACGCTGTTA 
CCACAGGAGT ACCGAAAGCT GCTCCGTAAT ATTCCGGAGG CAAATGCATG CAGCATTGCG 
GAGGACAATG CTCCGCAGAA GCCGCTTTTC TGTATGACTC CGCCCTTGCG CCGGGGTCGA 
GGAGTTACCT GTGATTGCGT GAACATGTTC AACACGCAGA CCTACAATAG CTCCAGTTCC 
GGAGGAAGTT CTAGTCAGCC GCCCAGCTCG GGCTACCGAG GTTCGTCTAC GATGTTGACG 
GACTCCGAGA TGGATTTGTT GGGACCGAGC GGTAGCGGTG GTCTTAGCAA TAACTTGTAC 
GTTTATCAGT ATGACAGCCC AGCGGAGGTG ACATCCGCGG AAAGGCCGCC TTCGGGACGG 
GTAGCACCGA AAGGTATCCT CAGGAAAACT TCAGTCCAAA GGCCTTCATC CAAGGCCAAG 
AGAAATAGCA TGTTTGAGGA GAATCACAAA TCTGGTCAGC ACCTGGAAAA ACGACGGTCC 
CTGCAGGAAC CGTGCTACAA CTTCTCGGAA GAGAACCTCA TCTTAGAGGA CTCAATCAAC 
GAACTCCCGG ACAGTGGTCT TCGCAAAATG AACAACTCTC CAAACATGTC CCAGCATCAA 
GCCCGTCTTC CCAACACACC CAAACTACCC TCGGCTGACG ACTATCATCG GTTGAACAAA 
CTTAACGAAA TGAATGATCT CGAACTGAAC AAAGCTGTCA GCAGCAAACT GAACCGTGCT 
CAGCAACAGC AGAGCTTGGA AGATGACTCG GAAGCCCGTA CGCGTACTGA GCATTTCCTG 
TCCAATGTTC CAAAATCAGA ACTGAAACAC TATGCCGAGC TCGCTCATTT CCTGGAAGCG 
ACCACAGCAG AAAATGCCAT AGCTCAGTCC GAGGAGGTTT ACGACCGTCA GCGGTTAAGG 
CACGAAGTCA GCCGTGCGTT ATCTCAACGC AAAAATGTGT CCTTCAACCA ACCCCCATCC 
GCCGGTCCCA TCAATACCAC TCCCGTCCGG CAACAGCAAG CTCAGCTGCT GCGACCGACG 
GAGATCAAGT TTTCAACTCC TCCAAACTCG CCAAACATGT CCGTTACGAT GGCACAGAAG 
CCACCGCAGA AGCCGACTCC TTCGGCGGTG CAATCCCCTT CAAGTGCCAC CGAACGGGAG 
AAGCAGGACA AGATCCAATC GAATCGTTTC AAGCGGCTGC AGATCCAGTG GGAACTGCTG 
AGCAAGGAGG GCCAACAGTT GAAGCAGGAA TTGCAGCAAC CGCGAAGCGG TGGCAACACT 
CCTACGGGAG GAGCAATGAA ATCTCGCATT CCGCGACCCG TCAGCTATCC GACCACTCGA 
GCAAACTCGG ATCCCATGGT GAGCAAAACG TTGAAATCGC CTAGCCGAAT TGTGCCGCCC 
AAGCGGTACA ATGCCGGTTC GCTGGGCACG GTAGCAGCGA TCGGTGGCCA CCAGCAGCAG 
GCAACGACTC CGCCCACTCC GGCTCCCCGG ACGCCCAACA AGCTACTGCA TACGACTCCA 
AAGAAGGCCG TCACCGCCGG TACGCCCAGG CCAGCAACAC GCACACGGAT ACCAGTAGGT 
TCTGGTCAAA CGCAAGGTAA ATCGCTTCCC TCGTCGAATG CAGCAGTGAC AGCAGCAAAG 
GTAACGCCGA AGAAACCCTT GAACCACGCG GCACCGGCTG CCAGTAGTAG TCCCAAGGCT 
ACCATCCGGA AACCGGTCAG CAGCAGTAGT GGCCGTGACG CCGAAAAACC AAACAAAGGA 
GTCGTCAAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021697717.1
CDS	391..5205
Translation	MLLCLFVFLWKILRNMSTISATIQQEQQLELLRQQLVEHQRRKLRQLQQQKPGSGNYQKLRKLCRDKAAATKENFLLHKFLFGSEAIPENGTVNGDKGGDGSCDKMDYLSDKDLKFLRMSEYPNRQQQQGKFVLSQQNQSLLNHTHNHIHQQFGRPLLSKQNSPIRRPLACNANFWPNGSNINANNANSNNNNNAHTTTPTGALSTGVHGLFGAPGINGGGGSPGRDSLLNDENLKSNVQIVLDNINRNYHALREQILSNSNHNNLNNNCNNNNGNVNPHHLVLLQRLQSVSQQLKRTAGLSGQQPQQYQGKMAASPDSDTNCNPYMPPLSCSIASSQDFSHDYSDYQWFMDYGSYRDGITHQSILSALSASYNGIGELSYYEDLAKNIDANLAEVDMESFRTEDIHSLLTTLPTMCSESAELRRNRNNNRGDLILDTWAEATGNHGAGITTVADLENSICKSKLLFSPVKESHISVDSLDMDGYPDEDDIILTCQANKNNYTIAFEQSALYSDESFYDGPENYAKYKHFNVLHNLDHIIRKKAQETAMSVSEVGYTTWSKLRKNGPGQRLPMVATNPLLNHISRHAPTCFVRKSMSMPDLKGKCDGTETDHHSEEVDHSAEASEHSHKDQQHRHQLNVSQAASSGATSGESFGKQMRTLLPMYPVPTDSGSESSVPLKEDCNLDNNEEQQQHQPSFNLVKLFIKQKSSSTDTCMDVSSGCWPSDSTNSASGGENNNNSNTNLAVASSKDRCRKKSMNDSGKCSNLGRHDEEEFQFDSLDAQINNNNNNNGTTPIANGDDRQDRTRLNDFNSPVHKRNYKAYNLNIANKNLPAVNPVVYNLMNNNNSGGSNASSGTNLSSNSINNNNNNNNSFKDTNSDASRTSENLTQIFNNSGNNNGDAAGAKRKVPIEMITKSMQTSFIGTKEKVRVVPPSFLEHLNKLGDKQKAPVFVVYPNYVLPDLGFLKSQNDVVISPLSFKENFAPRTHLRKTRPVSMNDIENLKAKEYKHVLDWKSLITLLPQEYRKLLRNIPEANACSIAEDNAPQKPLFCMTPPLRRGRGVTCDCVNMFNTQTYNSSSSGGSSSQPPSSGYRGSSTMLTDSEMDLLGPSGSGGLSNNLYVYQYDSPAEVTSAERPPSGRVAPKGILRKTSVQRPSSKAKRNSMFEENHKSGQHLEKRRSLQEPCYNFSEENLILEDSINELPDSGLRKMNNSPNMSQHQARLPNTPKLPSADDYHRLNKLNEMNDLELNKAVSSKLNRAQQQQSLEDDSEARTRTEHFLSNVPKSELKHYAELAHFLEATTAENAIAQSEEVYDRQRLRHEVSRALSQRKNVSFNQPPSAGPINTTPVRQQQAQLLRPTEIKFSTPPNSPNMSVTMAQKPPQKPTPSAVQSPSSATEREKQDKIQSNRFKRLQIQWELLSKEGQQLKQELQQPRSGGNTPTGGAMKSRIPRPVSYPTTRANSDPMVSKTLKSPSRIVPPKRYNAGSLGTVAAIGGHQQQATTPPTPAPRTPNKLLHTTPKKAVTAGTPRPATRTRIPVGSGQTQGKSLPSSNAAVTAAKVTPKKPLNHAAPAASSSPKATIRKPVSSSSGRDAEKPNKGVVKV

Target ORF information:

RefSeq Version	XM_021842025.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021842025.1

ORF Insert Sequence:

ATGCTCCTGT GTTTGTTTGT GTTTCTGTGG AAAATCCTAC GAAATATGTC CACCATATCG 
GCAACGATAC AGCAAGAGCA GCAACTGGAA CTGCTCCGGC AGCAGTTGGT AGAACATCAG 
CGAAGAAAGT TAAGACAGTT GCAACAGCAG AAGCCGGGCA GTGGCAATTA TCAAAAACTT 
CGAAAACTGT GTCGTGATAA GGCGGCAGCT ACCAAAGAAA ACTTTCTGCT GCACAAATTT 
TTGTTCGGGT CCGAGGCGAT CCCGGAGAAC GGAACGGTGA ATGGTGATAA GGGCGGTGAT 
GGTAGCTGTG ATAAAATGGA TTACTTGAGT GATAAAGATC TTAAATTTCT TCGTATGTCA 
GAGTACCCAA ACCGTCAACA GCAGCAGGGG AAGTTTGTGT TGTCACAGCA GAATCAGTCC 
CTTCTGAATC ATACTCATAA CCATATCCAT CAGCAGTTCG GTCGTCCGCT TCTCAGCAAG 
CAAAACTCAC CCATCCGACG TCCATTGGCT TGCAACGCAA ACTTCTGGCC CAACGGCAGC 
AATATCAACG CTAACAACGC CAACAGCAAC AACAACAACA ATGCCCATAC AACTACGCCA 
ACCGGAGCGT TGTCAACTGG AGTGCACGGC TTGTTTGGGG CACCCGGAAT CAACGGGGGC 
GGCGGTAGCC CAGGCCGTGA CAGTCTGTTA AACGACGAAA ACCTCAAATC GAACGTACAG 
ATTGTGCTGG ACAATATCAA TCGAAATTAT CATGCCCTGC GGGAGCAAAT ACTGAGCAAC 
AGTAATCATA ATAATCTCAA TAACAACTGC AACAACAACA ACGGTAACGT AAATCCTCAC 
CACCTAGTGC TGCTGCAGCG GCTGCAGAGT GTGAGTCAGC AGTTGAAGAG GACGGCAGGT 
TTGTCGGGAC AACAGCCGCA GCAGTACCAG GGCAAGATGG CGGCCAGTCC CGATTCGGAC 
ACGAACTGCA ATCCGTACAT GCCGCCGTTG AGCTGCTCGA TCGCCTCGTC GCAGGATTTC 
AGCCATGACT ACTCGGACTA TCAGTGGTTC ATGGATTACG GGAGCTATCG CGATGGAATC 
ACCCACCAGA GCATCCTGTC GGCGCTGTCC GCCTCGTACA ACGGCATCGG CGAGCTGTCC 
TACTACGAGG ACCTCGCCAA GAACATCGAC GCCAACCTGG CCGAGGTGGA CATGGAGAGC 
TTCCGCACCG AAGACATCCA CTCGTTGCTC ACGACGCTGC CGACGATGTG CAGCGAAAGT 
GCCGAACTGC GTCGCAATCG GAACAACAAC CGGGGCGATC TGATCCTGGA CACGTGGGCC 
GAAGCTACGG GGAATCATGG GGCCGGGATC ACAACGGTGG CCGACCTGGA GAACTCGATC 
TGCAAGTCGA AATTGCTATT CTCGCCGGTG AAGGAGTCGC ACATCAGTGT GGACTCGTTG 
GACATGGATG GGTATCCGGA CGAGGATGAT ATCATACTGA CGTGCCAGGC GAACAAGAAC 
AACTACACGA TCGCGTTCGA GCAGAGCGCG CTTTACTCGG ACGAGAGTTT CTACGATGGT 
CCGGAGAATT ATGCCAAATA CAAGCATTTC AATGTGCTTC ACAACCTGGA TCACATCATC 
AGGAAGAAGG CTCAGGAAAC GGCGATGTCC GTTTCGGAAG TGGGTTACAC GACTTGGAGT 
AAGCTCAGGA AAAATGGACC GGGACAAAGG CTCCCAATGG TGGCGACAAA TCCGTTACTC 
AACCACATTT CCAGGCATGC ACCGACTTGT TTCGTACGAA AGAGCATGAG CATGCCAGAT 
CTTAAAGGAA AGTGCGATGG AACGGAAACA GATCATCATT CCGAAGAAGT AGATCACAGC 
GCAGAGGCAT CAGAACACTC GCATAAGGAT CAGCAGCACA GGCATCAGTT GAACGTATCG 
CAAGCGGCAA GCTCCGGAGC TACTTCCGGT GAGTCGTTCG GTAAGCAGAT GCGGACACTA 
CTACCGATGT ATCCGGTGCC CACCGATTCC GGCAGCGAGA GTTCCGTTCC TCTGAAGGAG 
GACTGCAACT TGGACAATAA TGAGGAGCAG CAACAGCATC AACCGTCGTT CAATTTGGTG 
AAGTTATTCA TCAAACAGAA AAGCAGTTCG ACAGACACCT GTATGGATGT TTCGTCGGGT 
TGTTGGCCGA GCGATTCTAC GAATAGTGCT TCCGGTGGTG AGAACAACAA CAACAGTAAC 
ACCAATTTGG CTGTAGCCAG TTCAAAGGAT CGTTGTCGGA AGAAAAGCAT GAACGATTCG 
GGAAAATGTT CGAACCTGGG AAGACACGAC GAGGAAGAGT TCCAGTTCGA TAGTCTGGAT 
GCACAAATCA ATAACAATAA CAACAACAAT GGGACAACGC CGATCGCCAA TGGGGACGAT 
AGGCAGGATC GGACGAGGTT GAACGATTTC AACTCCCCGG TTCATAAGAG GAACTACAAG 
GCATACAATC TGAACATCGC CAACAAGAAT CTCCCTGCGG TGAATCCGGT GGTGTACAAC 
CTAATGAACA ACAACAACAG TGGAGGAAGC AACGCCAGCA GCGGAACCAA TCTGAGCAGC 
AACAGTATTA ACAACAATAA TAATAATAAC AATAGCTTTA AGGATACCAA CTCGGATGCT 
AGTCGTACGT CGGAGAATCT GACGCAAATC TTCAACAACA GTGGCAATAA CAATGGCGAC 
GCTGCCGGTG CCAAAAGGAA GGTACCGATC GAGATGATTA CCAAGTCGAT GCAGACGTCG 
TTCATCGGGA CCAAGGAGAA GGTCCGCGTG GTACCTCCAT CATTCCTGGA GCACCTGAAC 
AAACTCGGTG ACAAACAGAA GGCCCCGGTC TTCGTTGTGT ACCCGAACTA CGTCCTCCCG 
GATTTGGGCT TCCTGAAGTC GCAAAACGAT GTCGTCATTT CGCCGCTCTC GTTCAAAGAG 
AACTTCGCCC CGCGAACGCA CCTTCGCAAA ACGCGGCCAG TTTCGATGAA CGACATCGAA 
AACCTCAAAG CCAAGGAATA CAAGCACGTG TTGGACTGGA AGTCGCTCAT AACGCTGTTA 
CCACAGGAGT ACCGAAAGCT GCTCCGTAAT ATTCCGGAGG CAAATGCATG CAGCATTGCG 
GAGGACAATG CTCCGCAGAA GCCGCTTTTC TGTATGACTC CGCCCTTGCG CCGGGGTCGA 
GGAGTTACCT GTGATTGCGT GAACATGTTC AACACGCAGA CCTACAATAG CTCCAGTTCC 
GGAGGAAGTT CTAGTCAGCC GCCCAGCTCG GGCTACCGAG GTTCGTCTAC GATGTTGACG 
GACTCCGAGA TGGATTTGTT GGGACCGAGC GGTAGCGGTG GTCTTAGCAA TAACTTGTAC 
GTTTATCAGT ATGACAGCCC AGCGGAGGTG ACATCCGCGG AAAGGCCGCC TTCGGGACGG 
GTAGCACCGA AAGGTATCCT CAGGAAAACT TCAGTCCAAA GGCCTTCATC CAAGGCCAAG 
AGAAATAGCA TGTTTGAGGA GAATCACAAA TCTGGTCAGC ACCTGGAAAA ACGACGGTCC 
CTGCAGGAAC CGTGCTACAA CTTCTCGGAA GAGAACCTCA TCTTAGAGGA CTCAATCAAC 
GAACTCCCGG ACAGTGGTCT TCGCAAAATG AACAACTCTC CAAACATGTC CCAGCATCAA 
GCCCGTCTTC CCAACACACC CAAACTACCC TCGGCTGACG ACTATCATCG GTTGAACAAA 
CTTAACGAAA TGAATGATCT CGAACTGAAC AAAGCTGTCA GCAGCAAACT GAACCGTGCT 
CAGCAACAGC AGAGCTTGGA AGATGACTCG GAAGCCCGTA CGCGTACTGA GCATTTCCTG 
TCCAATGTTC CAAAATCAGA ACTGAAACAC TATGCCGAGC TCGCTCATTT CCTGGAAGCG 
ACCACAGCAG AAAATGCCAT AGCTCAGTCC GAGGAGGTTT ACGACCGTCA GCGGTTAAGG 
CACGAAGTCA GCCGTGCGTT ATCTCAACGC AAAAATGTGT CCTTCAACCA ACCCCCATCC 
GCCGGTCCCA TCAATACCAC TCCCGTCCGG CAACAGCAAG CTCAGCTGCT GCGACCGACG 
GAGATCAAGT TTTCAACTCC TCCAAACTCG CCAAACATGT CCGTTACGAT GGCACAGAAG 
CCACCGCAGA AGCCGACTCC TTCGGCGGTG CAATCCCCTT CAAGTGCCAC CGAACGGGAG 
AAGCAGGACA AGATCCAATC GAATCGTTTC AAGCGGCTGC AGATCCAGTG GGAACTGCTG 
AGCAAGGAGG GCCAACAGTT GAAGCAGGAA TTGCAGCAAC CGCGAAGCGG TGGCAACACT 
CCTACGGGAG GAGCAATGAA ATCTCGCATT CCGCGACCCG TCAGCTATCC GACCACTCGA 
GCAAACTCGG ATCCCATGGT GAGCAAAACG TTGAAATCGC CTAGCCGAAT TGTGCCGCCC 
AAGCGGTACA ATGCCGGTTC GCTGGGCACG GTAGCAGCGA TCGGTGGCCA CCAGCAGCAG 
GCAACGACTC CGCCCACTCC GGCTCCCCGG ACGCCCAACA AGCTACTGCA TACGACTCCA 
AAGAAGGCCG TCACCGCCGG TACGCCCAGG CCAGCAACAC GCACACGGAT ACCAGTAGGT 
TCTGGTCAAA CGCAAGGTAA ATCGCTTCCC TCGTCGAATG CAGCAGTGAC AGCAGCAAAG 
GTAACGCCGA AGAAACCCTT GAACCACGCG GCACCGGCTG CCAGTAGTAG TCCCAAGGCT 
ACCATCCGGA AACCGGTCAG CAGCAGTAGT GGCCGTGACG CCGAAAAACC AAACAAAGGA 
GTCGTCAAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5574982 ( XM_021842029.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021697721.1 Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X3, mRNA.

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CloneID	OAe131558
Clone ID Related Accession (Same CDS sequence)	XM_021842025.1 , XM_021842029.1 , XM_021842027.1 , XM_021842032.1
Accession Version	XM_021842029.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-19
Organism	Aedes aegypti(yellow fever mosquito)
Product	uncharacterized protein LOC5574982 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGT GTTTGTTTGT GTTTCTGTGG AAAATCCTAC GAAATATGTC CACCATATCG 
GCAACGATAC AGCAAGAGCA GCAACTGGAA CTGCTCCGGC AGCAGTTGGT AGAACATCAG 
CGAAGAAAGT TAAGACAGTT GCAACAGCAG AAGCCGGGCA GTGGCAATTA TCAAAAACTT 
CGAAAACTGT GTCGTGATAA GGCGGCAGCT ACCAAAGAAA ACTTTCTGCT GCACAAATTT 
TTGTTCGGGT CCGAGGCGAT CCCGGAGAAC GGAACGGTGA ATGGTGATAA GGGCGGTGAT 
GGTAGCTGTG ATAAAATGGA TTACTTGAGT GATAAAGATC TTAAATTTCT TCGTATGTCA 
GAGTACCCAA ACCGTCAACA GCAGCAGGGG AAGTTTGTGT TGTCACAGCA GAATCAGTCC 
CTTCTGAATC ATACTCATAA CCATATCCAT CAGCAGTTCG GTCGTCCGCT TCTCAGCAAG 
CAAAACTCAC CCATCCGACG TCCATTGGCT TGCAACGCAA ACTTCTGGCC CAACGGCAGC 
AATATCAACG CTAACAACGC CAACAGCAAC AACAACAACA ATGCCCATAC AACTACGCCA 
ACCGGAGCGT TGTCAACTGG AGTGCACGGC TTGTTTGGGG CACCCGGAAT CAACGGGGGC 
GGCGGTAGCC CAGGCCGTGA CAGTCTGTTA AACGACGAAA ACCTCAAATC GAACGTACAG 
ATTGTGCTGG ACAATATCAA TCGAAATTAT CATGCCCTGC GGGAGCAAAT ACTGAGCAAC 
AGTAATCATA ATAATCTCAA TAACAACTGC AACAACAACA ACGGTAACGT AAATCCTCAC 
CACCTAGTGC TGCTGCAGCG GCTGCAGAGT GTGAGTCAGC AGTTGAAGAG GACGGCAGGT 
TTGTCGGGAC AACAGCCGCA GCAGTACCAG GGCAAGATGG CGGCCAGTCC CGATTCGGAC 
ACGAACTGCA ATCCGTACAT GCCGCCGTTG AGCTGCTCGA TCGCCTCGTC GCAGGATTTC 
AGCCATGACT ACTCGGACTA TCAGTGGTTC ATGGATTACG GGAGCTATCG CGATGGAATC 
ACCCACCAGA GCATCCTGTC GGCGCTGTCC GCCTCGTACA ACGGCATCGG CGAGCTGTCC 
TACTACGAGG ACCTCGCCAA GAACATCGAC GCCAACCTGG CCGAGGTGGA CATGGAGAGC 
TTCCGCACCG AAGACATCCA CTCGTTGCTC ACGACGCTGC CGACGATGTG CAGCGAAAGT 
GCCGAACTGC GTCGCAATCG GAACAACAAC CGGGGCGATC TGATCCTGGA CACGTGGGCC 
GAAGCTACGG GGAATCATGG GGCCGGGATC ACAACGGTGG CCGACCTGGA GAACTCGATC 
TGCAAGTCGA AATTGCTATT CTCGCCGGTG AAGGAGTCGC ACATCAGTGT GGACTCGTTG 
GACATGGATG GGTATCCGGA CGAGGATGAT ATCATACTGA CGTGCCAGGC GAACAAGAAC 
AACTACACGA TCGCGTTCGA GCAGAGCGCG CTTTACTCGG ACGAGAGTTT CTACGATGGT 
CCGGAGAATT ATGCCAAATA CAAGCATTTC AATGTGCTTC ACAACCTGGA TCACATCATC 
AGGAAGAAGG CTCAGGAAAC GGCGATGTCC GTTTCGGAAG TGGGTTACAC GACTTGGAGT 
AAGCTCAGGA AAAATGGACC GGGACAAAGG CTCCCAATGG TGGCGACAAA TCCGTTACTC 
AACCACATTT CCAGGCATGC ACCGACTTGT TTCGTACGAA AGAGCATGAG CATGCCAGAT 
CTTAAAGGAA AGTGCGATGG AACGGAAACA GATCATCATT CCGAAGAAGT AGATCACAGC 
GCAGAGGCAT CAGAACACTC GCATAAGGAT CAGCAGCACA GGCATCAGTT GAACGTATCG 
CAAGCGGCAA GCTCCGGAGC TACTTCCGGT GAGTCGTTCG GTAAGCAGAT GCGGACACTA 
CTACCGATGT ATCCGGTGCC CACCGATTCC GGCAGCGAGA GTTCCGTTCC TCTGAAGGAG 
GACTGCAACT TGGACAATAA TGAGGAGCAG CAACAGCATC AACCGTCGTT CAATTTGGTG 
AAGTTATTCA TCAAACAGAA AAGCAGTTCG ACAGACACCT GTATGGATGT TTCGTCGGGT 
TGTTGGCCGA GCGATTCTAC GAATAGTGCT TCCGGTGGTG AGAACAACAA CAACAGTAAC 
ACCAATTTGG CTGTAGCCAG TTCAAAGGAT CGTTGTCGGA AGAAAAGCAT GAACGATTCG 
GGAAAATGTT CGAACCTGGG AAGACACGAC GAGGAAGAGT TCCAGTTCGA TAGTCTGGAT 
GCACAAATCA ATAACAATAA CAACAACAAT GGGACAACGC CGATCGCCAA TGGGGACGAT 
AGGCAGGATC GGACGAGGTT GAACGATTTC AACTCCCCGG TTCATAAGAG GAACTACAAG 
GCATACAATC TGAACATCGC CAACAAGAAT CTCCCTGCGG TGAATCCGGT GGTGTACAAC 
CTAATGAACA ACAACAACAG TGGAGGAAGC AACGCCAGCA GCGGAACCAA TCTGAGCAGC 
AACAGTATTA ACAACAATAA TAATAATAAC AATAGCTTTA AGGATACCAA CTCGGATGCT 
AGTCGTACGT CGGAGAATCT GACGCAAATC TTCAACAACA GTGGCAATAA CAATGGCGAC 
GCTGCCGGTG CCAAAAGGAA GGTACCGATC GAGATGATTA CCAAGTCGAT GCAGACGTCG 
TTCATCGGGA CCAAGGAGAA GGTCCGCGTG GTACCTCCAT CATTCCTGGA GCACCTGAAC 
AAACTCGGTG ACAAACAGAA GGCCCCGGTC TTCGTTGTGT ACCCGAACTA CGTCCTCCCG 
GATTTGGGCT TCCTGAAGTC GCAAAACGAT GTCGTCATTT CGCCGCTCTC GTTCAAAGAG 
AACTTCGCCC CGCGAACGCA CCTTCGCAAA ACGCGGCCAG TTTCGATGAA CGACATCGAA 
AACCTCAAAG CCAAGGAATA CAAGCACGTG TTGGACTGGA AGTCGCTCAT AACGCTGTTA 
CCACAGGAGT ACCGAAAGCT GCTCCGTAAT ATTCCGGAGG CAAATGCATG CAGCATTGCG 
GAGGACAATG CTCCGCAGAA GCCGCTTTTC TGTATGACTC CGCCCTTGCG CCGGGGTCGA 
GGAGTTACCT GTGATTGCGT GAACATGTTC AACACGCAGA CCTACAATAG CTCCAGTTCC 
GGAGGAAGTT CTAGTCAGCC GCCCAGCTCG GGCTACCGAG GTTCGTCTAC GATGTTGACG 
GACTCCGAGA TGGATTTGTT GGGACCGAGC GGTAGCGGTG GTCTTAGCAA TAACTTGTAC 
GTTTATCAGT ATGACAGCCC AGCGGAGGTG ACATCCGCGG AAAGGCCGCC TTCGGGACGG 
GTAGCACCGA AAGGTATCCT CAGGAAAACT TCAGTCCAAA GGCCTTCATC CAAGGCCAAG 
AGAAATAGCA TGTTTGAGGA GAATCACAAA TCTGGTCAGC ACCTGGAAAA ACGACGGTCC 
CTGCAGGAAC CGTGCTACAA CTTCTCGGAA GAGAACCTCA TCTTAGAGGA CTCAATCAAC 
GAACTCCCGG ACAGTGGTCT TCGCAAAATG AACAACTCTC CAAACATGTC CCAGCATCAA 
GCCCGTCTTC CCAACACACC CAAACTACCC TCGGCTGACG ACTATCATCG GTTGAACAAA 
CTTAACGAAA TGAATGATCT CGAACTGAAC AAAGCTGTCA GCAGCAAACT GAACCGTGCT 
CAGCAACAGC AGAGCTTGGA AGATGACTCG GAAGCCCGTA CGCGTACTGA GCATTTCCTG 
TCCAATGTTC CAAAATCAGA ACTGAAACAC TATGCCGAGC TCGCTCATTT CCTGGAAGCG 
ACCACAGCAG AAAATGCCAT AGCTCAGTCC GAGGAGGTTT ACGACCGTCA GCGGTTAAGG 
CACGAAGTCA GCCGTGCGTT ATCTCAACGC AAAAATGTGT CCTTCAACCA ACCCCCATCC 
GCCGGTCCCA TCAATACCAC TCCCGTCCGG CAACAGCAAG CTCAGCTGCT GCGACCGACG 
GAGATCAAGT TTTCAACTCC TCCAAACTCG CCAAACATGT CCGTTACGAT GGCACAGAAG 
CCACCGCAGA AGCCGACTCC TTCGGCGGTG CAATCCCCTT CAAGTGCCAC CGAACGGGAG 
AAGCAGGACA AGATCCAATC GAATCGTTTC AAGCGGCTGC AGATCCAGTG GGAACTGCTG 
AGCAAGGAGG GCCAACAGTT GAAGCAGGAA TTGCAGCAAC CGCGAAGCGG TGGCAACACT 
CCTACGGGAG GAGCAATGAA ATCTCGCATT CCGCGACCCG TCAGCTATCC GACCACTCGA 
GCAAACTCGG ATCCCATGGT GAGCAAAACG TTGAAATCGC CTAGCCGAAT TGTGCCGCCC 
AAGCGGTACA ATGCCGGTTC GCTGGGCACG GTAGCAGCGA TCGGTGGCCA CCAGCAGCAG 
GCAACGACTC CGCCCACTCC GGCTCCCCGG ACGCCCAACA AGCTACTGCA TACGACTCCA 
AAGAAGGCCG TCACCGCCGG TACGCCCAGG CCAGCAACAC GCACACGGAT ACCAGTAGGT 
TCTGGTCAAA CGCAAGGTAA ATCGCTTCCC TCGTCGAATG CAGCAGTGAC AGCAGCAAAG 
GTAACGCCGA AGAAACCCTT GAACCACGCG GCACCGGCTG CCAGTAGTAG TCCCAAGGCT 
ACCATCCGGA AACCGGTCAG CAGCAGTAGT GGCCGTGACG CCGAAAAACC AAACAAAGGA 
GTCGTCAAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021697721.1
CDS	181..4995
Translation	MLLCLFVFLWKILRNMSTISATIQQEQQLELLRQQLVEHQRRKLRQLQQQKPGSGNYQKLRKLCRDKAAATKENFLLHKFLFGSEAIPENGTVNGDKGGDGSCDKMDYLSDKDLKFLRMSEYPNRQQQQGKFVLSQQNQSLLNHTHNHIHQQFGRPLLSKQNSPIRRPLACNANFWPNGSNINANNANSNNNNNAHTTTPTGALSTGVHGLFGAPGINGGGGSPGRDSLLNDENLKSNVQIVLDNINRNYHALREQILSNSNHNNLNNNCNNNNGNVNPHHLVLLQRLQSVSQQLKRTAGLSGQQPQQYQGKMAASPDSDTNCNPYMPPLSCSIASSQDFSHDYSDYQWFMDYGSYRDGITHQSILSALSASYNGIGELSYYEDLAKNIDANLAEVDMESFRTEDIHSLLTTLPTMCSESAELRRNRNNNRGDLILDTWAEATGNHGAGITTVADLENSICKSKLLFSPVKESHISVDSLDMDGYPDEDDIILTCQANKNNYTIAFEQSALYSDESFYDGPENYAKYKHFNVLHNLDHIIRKKAQETAMSVSEVGYTTWSKLRKNGPGQRLPMVATNPLLNHISRHAPTCFVRKSMSMPDLKGKCDGTETDHHSEEVDHSAEASEHSHKDQQHRHQLNVSQAASSGATSGESFGKQMRTLLPMYPVPTDSGSESSVPLKEDCNLDNNEEQQQHQPSFNLVKLFIKQKSSSTDTCMDVSSGCWPSDSTNSASGGENNNNSNTNLAVASSKDRCRKKSMNDSGKCSNLGRHDEEEFQFDSLDAQINNNNNNNGTTPIANGDDRQDRTRLNDFNSPVHKRNYKAYNLNIANKNLPAVNPVVYNLMNNNNSGGSNASSGTNLSSNSINNNNNNNNSFKDTNSDASRTSENLTQIFNNSGNNNGDAAGAKRKVPIEMITKSMQTSFIGTKEKVRVVPPSFLEHLNKLGDKQKAPVFVVYPNYVLPDLGFLKSQNDVVISPLSFKENFAPRTHLRKTRPVSMNDIENLKAKEYKHVLDWKSLITLLPQEYRKLLRNIPEANACSIAEDNAPQKPLFCMTPPLRRGRGVTCDCVNMFNTQTYNSSSSGGSSSQPPSSGYRGSSTMLTDSEMDLLGPSGSGGLSNNLYVYQYDSPAEVTSAERPPSGRVAPKGILRKTSVQRPSSKAKRNSMFEENHKSGQHLEKRRSLQEPCYNFSEENLILEDSINELPDSGLRKMNNSPNMSQHQARLPNTPKLPSADDYHRLNKLNEMNDLELNKAVSSKLNRAQQQQSLEDDSEARTRTEHFLSNVPKSELKHYAELAHFLEATTAENAIAQSEEVYDRQRLRHEVSRALSQRKNVSFNQPPSAGPINTTPVRQQQAQLLRPTEIKFSTPPNSPNMSVTMAQKPPQKPTPSAVQSPSSATEREKQDKIQSNRFKRLQIQWELLSKEGQQLKQELQQPRSGGNTPTGGAMKSRIPRPVSYPTTRANSDPMVSKTLKSPSRIVPPKRYNAGSLGTVAAIGGHQQQATTPPTPAPRTPNKLLHTTPKKAVTAGTPRPATRTRIPVGSGQTQGKSLPSSNAAVTAAKVTPKKPLNHAAPAASSSPKATIRKPVSSSSGRDAEKPNKGVVKV

Target ORF information:

RefSeq Version	XM_021842029.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021842029.1

ORF Insert Sequence:

ATGCTCCTGT GTTTGTTTGT GTTTCTGTGG AAAATCCTAC GAAATATGTC CACCATATCG 
GCAACGATAC AGCAAGAGCA GCAACTGGAA CTGCTCCGGC AGCAGTTGGT AGAACATCAG 
CGAAGAAAGT TAAGACAGTT GCAACAGCAG AAGCCGGGCA GTGGCAATTA TCAAAAACTT 
CGAAAACTGT GTCGTGATAA GGCGGCAGCT ACCAAAGAAA ACTTTCTGCT GCACAAATTT 
TTGTTCGGGT CCGAGGCGAT CCCGGAGAAC GGAACGGTGA ATGGTGATAA GGGCGGTGAT 
GGTAGCTGTG ATAAAATGGA TTACTTGAGT GATAAAGATC TTAAATTTCT TCGTATGTCA 
GAGTACCCAA ACCGTCAACA GCAGCAGGGG AAGTTTGTGT TGTCACAGCA GAATCAGTCC 
CTTCTGAATC ATACTCATAA CCATATCCAT CAGCAGTTCG GTCGTCCGCT TCTCAGCAAG 
CAAAACTCAC CCATCCGACG TCCATTGGCT TGCAACGCAA ACTTCTGGCC CAACGGCAGC 
AATATCAACG CTAACAACGC CAACAGCAAC AACAACAACA ATGCCCATAC AACTACGCCA 
ACCGGAGCGT TGTCAACTGG AGTGCACGGC TTGTTTGGGG CACCCGGAAT CAACGGGGGC 
GGCGGTAGCC CAGGCCGTGA CAGTCTGTTA AACGACGAAA ACCTCAAATC GAACGTACAG 
ATTGTGCTGG ACAATATCAA TCGAAATTAT CATGCCCTGC GGGAGCAAAT ACTGAGCAAC 
AGTAATCATA ATAATCTCAA TAACAACTGC AACAACAACA ACGGTAACGT AAATCCTCAC 
CACCTAGTGC TGCTGCAGCG GCTGCAGAGT GTGAGTCAGC AGTTGAAGAG GACGGCAGGT 
TTGTCGGGAC AACAGCCGCA GCAGTACCAG GGCAAGATGG CGGCCAGTCC CGATTCGGAC 
ACGAACTGCA ATCCGTACAT GCCGCCGTTG AGCTGCTCGA TCGCCTCGTC GCAGGATTTC 
AGCCATGACT ACTCGGACTA TCAGTGGTTC ATGGATTACG GGAGCTATCG CGATGGAATC 
ACCCACCAGA GCATCCTGTC GGCGCTGTCC GCCTCGTACA ACGGCATCGG CGAGCTGTCC 
TACTACGAGG ACCTCGCCAA GAACATCGAC GCCAACCTGG CCGAGGTGGA CATGGAGAGC 
TTCCGCACCG AAGACATCCA CTCGTTGCTC ACGACGCTGC CGACGATGTG CAGCGAAAGT 
GCCGAACTGC GTCGCAATCG GAACAACAAC CGGGGCGATC TGATCCTGGA CACGTGGGCC 
GAAGCTACGG GGAATCATGG GGCCGGGATC ACAACGGTGG CCGACCTGGA GAACTCGATC 
TGCAAGTCGA AATTGCTATT CTCGCCGGTG AAGGAGTCGC ACATCAGTGT GGACTCGTTG 
GACATGGATG GGTATCCGGA CGAGGATGAT ATCATACTGA CGTGCCAGGC GAACAAGAAC 
AACTACACGA TCGCGTTCGA GCAGAGCGCG CTTTACTCGG ACGAGAGTTT CTACGATGGT 
CCGGAGAATT ATGCCAAATA CAAGCATTTC AATGTGCTTC ACAACCTGGA TCACATCATC 
AGGAAGAAGG CTCAGGAAAC GGCGATGTCC GTTTCGGAAG TGGGTTACAC GACTTGGAGT 
AAGCTCAGGA AAAATGGACC GGGACAAAGG CTCCCAATGG TGGCGACAAA TCCGTTACTC 
AACCACATTT CCAGGCATGC ACCGACTTGT TTCGTACGAA AGAGCATGAG CATGCCAGAT 
CTTAAAGGAA AGTGCGATGG AACGGAAACA GATCATCATT CCGAAGAAGT AGATCACAGC 
GCAGAGGCAT CAGAACACTC GCATAAGGAT CAGCAGCACA GGCATCAGTT GAACGTATCG 
CAAGCGGCAA GCTCCGGAGC TACTTCCGGT GAGTCGTTCG GTAAGCAGAT GCGGACACTA 
CTACCGATGT ATCCGGTGCC CACCGATTCC GGCAGCGAGA GTTCCGTTCC TCTGAAGGAG 
GACTGCAACT TGGACAATAA TGAGGAGCAG CAACAGCATC AACCGTCGTT CAATTTGGTG 
AAGTTATTCA TCAAACAGAA AAGCAGTTCG ACAGACACCT GTATGGATGT TTCGTCGGGT 
TGTTGGCCGA GCGATTCTAC GAATAGTGCT TCCGGTGGTG AGAACAACAA CAACAGTAAC 
ACCAATTTGG CTGTAGCCAG TTCAAAGGAT CGTTGTCGGA AGAAAAGCAT GAACGATTCG 
GGAAAATGTT CGAACCTGGG AAGACACGAC GAGGAAGAGT TCCAGTTCGA TAGTCTGGAT 
GCACAAATCA ATAACAATAA CAACAACAAT GGGACAACGC CGATCGCCAA TGGGGACGAT 
AGGCAGGATC GGACGAGGTT GAACGATTTC AACTCCCCGG TTCATAAGAG GAACTACAAG 
GCATACAATC TGAACATCGC CAACAAGAAT CTCCCTGCGG TGAATCCGGT GGTGTACAAC 
CTAATGAACA ACAACAACAG TGGAGGAAGC AACGCCAGCA GCGGAACCAA TCTGAGCAGC 
AACAGTATTA ACAACAATAA TAATAATAAC AATAGCTTTA AGGATACCAA CTCGGATGCT 
AGTCGTACGT CGGAGAATCT GACGCAAATC TTCAACAACA GTGGCAATAA CAATGGCGAC 
GCTGCCGGTG CCAAAAGGAA GGTACCGATC GAGATGATTA CCAAGTCGAT GCAGACGTCG 
TTCATCGGGA CCAAGGAGAA GGTCCGCGTG GTACCTCCAT CATTCCTGGA GCACCTGAAC 
AAACTCGGTG ACAAACAGAA GGCCCCGGTC TTCGTTGTGT ACCCGAACTA CGTCCTCCCG 
GATTTGGGCT TCCTGAAGTC GCAAAACGAT GTCGTCATTT CGCCGCTCTC GTTCAAAGAG 
AACTTCGCCC CGCGAACGCA CCTTCGCAAA ACGCGGCCAG TTTCGATGAA CGACATCGAA 
AACCTCAAAG CCAAGGAATA CAAGCACGTG TTGGACTGGA AGTCGCTCAT AACGCTGTTA 
CCACAGGAGT ACCGAAAGCT GCTCCGTAAT ATTCCGGAGG CAAATGCATG CAGCATTGCG 
GAGGACAATG CTCCGCAGAA GCCGCTTTTC TGTATGACTC CGCCCTTGCG CCGGGGTCGA 
GGAGTTACCT GTGATTGCGT GAACATGTTC AACACGCAGA CCTACAATAG CTCCAGTTCC 
GGAGGAAGTT CTAGTCAGCC GCCCAGCTCG GGCTACCGAG GTTCGTCTAC GATGTTGACG 
GACTCCGAGA TGGATTTGTT GGGACCGAGC GGTAGCGGTG GTCTTAGCAA TAACTTGTAC 
GTTTATCAGT ATGACAGCCC AGCGGAGGTG ACATCCGCGG AAAGGCCGCC TTCGGGACGG 
GTAGCACCGA AAGGTATCCT CAGGAAAACT TCAGTCCAAA GGCCTTCATC CAAGGCCAAG 
AGAAATAGCA TGTTTGAGGA GAATCACAAA TCTGGTCAGC ACCTGGAAAA ACGACGGTCC 
CTGCAGGAAC CGTGCTACAA CTTCTCGGAA GAGAACCTCA TCTTAGAGGA CTCAATCAAC 
GAACTCCCGG ACAGTGGTCT TCGCAAAATG AACAACTCTC CAAACATGTC CCAGCATCAA 
GCCCGTCTTC CCAACACACC CAAACTACCC TCGGCTGACG ACTATCATCG GTTGAACAAA 
CTTAACGAAA TGAATGATCT CGAACTGAAC AAAGCTGTCA GCAGCAAACT GAACCGTGCT 
CAGCAACAGC AGAGCTTGGA AGATGACTCG GAAGCCCGTA CGCGTACTGA GCATTTCCTG 
TCCAATGTTC CAAAATCAGA ACTGAAACAC TATGCCGAGC TCGCTCATTT CCTGGAAGCG 
ACCACAGCAG AAAATGCCAT AGCTCAGTCC GAGGAGGTTT ACGACCGTCA GCGGTTAAGG 
CACGAAGTCA GCCGTGCGTT ATCTCAACGC AAAAATGTGT CCTTCAACCA ACCCCCATCC 
GCCGGTCCCA TCAATACCAC TCCCGTCCGG CAACAGCAAG CTCAGCTGCT GCGACCGACG 
GAGATCAAGT TTTCAACTCC TCCAAACTCG CCAAACATGT CCGTTACGAT GGCACAGAAG 
CCACCGCAGA AGCCGACTCC TTCGGCGGTG CAATCCCCTT CAAGTGCCAC CGAACGGGAG 
AAGCAGGACA AGATCCAATC GAATCGTTTC AAGCGGCTGC AGATCCAGTG GGAACTGCTG 
AGCAAGGAGG GCCAACAGTT GAAGCAGGAA TTGCAGCAAC CGCGAAGCGG TGGCAACACT 
CCTACGGGAG GAGCAATGAA ATCTCGCATT CCGCGACCCG TCAGCTATCC GACCACTCGA 
GCAAACTCGG ATCCCATGGT GAGCAAAACG TTGAAATCGC CTAGCCGAAT TGTGCCGCCC 
AAGCGGTACA ATGCCGGTTC GCTGGGCACG GTAGCAGCGA TCGGTGGCCA CCAGCAGCAG 
GCAACGACTC CGCCCACTCC GGCTCCCCGG ACGCCCAACA AGCTACTGCA TACGACTCCA 
AAGAAGGCCG TCACCGCCGG TACGCCCAGG CCAGCAACAC GCACACGGAT ACCAGTAGGT 
TCTGGTCAAA CGCAAGGTAA ATCGCTTCCC TCGTCGAATG CAGCAGTGAC AGCAGCAAAG 
GTAACGCCGA AGAAACCCTT GAACCACGCG GCACCGGCTG CCAGTAGTAG TCCCAAGGCT 
ACCATCCGGA AACCGGTCAG CAGCAGTAGT GGCCGTGACG CCGAAAAACC AAACAAAGGA 
GTCGTCAAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5574982 ( XM_021842027.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021697719.1 Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X2, mRNA.

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CloneID	OAe131558
Clone ID Related Accession (Same CDS sequence)	XM_021842025.1 , XM_021842029.1 , XM_021842027.1 , XM_021842032.1
Accession Version	XM_021842027.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-19
Organism	Aedes aegypti(yellow fever mosquito)
Product	uncharacterized protein LOC5574982 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGT GTTTGTTTGT GTTTCTGTGG AAAATCCTAC GAAATATGTC CACCATATCG 
GCAACGATAC AGCAAGAGCA GCAACTGGAA CTGCTCCGGC AGCAGTTGGT AGAACATCAG 
CGAAGAAAGT TAAGACAGTT GCAACAGCAG AAGCCGGGCA GTGGCAATTA TCAAAAACTT 
CGAAAACTGT GTCGTGATAA GGCGGCAGCT ACCAAAGAAA ACTTTCTGCT GCACAAATTT 
TTGTTCGGGT CCGAGGCGAT CCCGGAGAAC GGAACGGTGA ATGGTGATAA GGGCGGTGAT 
GGTAGCTGTG ATAAAATGGA TTACTTGAGT GATAAAGATC TTAAATTTCT TCGTATGTCA 
GAGTACCCAA ACCGTCAACA GCAGCAGGGG AAGTTTGTGT TGTCACAGCA GAATCAGTCC 
CTTCTGAATC ATACTCATAA CCATATCCAT CAGCAGTTCG GTCGTCCGCT TCTCAGCAAG 
CAAAACTCAC CCATCCGACG TCCATTGGCT TGCAACGCAA ACTTCTGGCC CAACGGCAGC 
AATATCAACG CTAACAACGC CAACAGCAAC AACAACAACA ATGCCCATAC AACTACGCCA 
ACCGGAGCGT TGTCAACTGG AGTGCACGGC TTGTTTGGGG CACCCGGAAT CAACGGGGGC 
GGCGGTAGCC CAGGCCGTGA CAGTCTGTTA AACGACGAAA ACCTCAAATC GAACGTACAG 
ATTGTGCTGG ACAATATCAA TCGAAATTAT CATGCCCTGC GGGAGCAAAT ACTGAGCAAC 
AGTAATCATA ATAATCTCAA TAACAACTGC AACAACAACA ACGGTAACGT AAATCCTCAC 
CACCTAGTGC TGCTGCAGCG GCTGCAGAGT GTGAGTCAGC AGTTGAAGAG GACGGCAGGT 
TTGTCGGGAC AACAGCCGCA GCAGTACCAG GGCAAGATGG CGGCCAGTCC CGATTCGGAC 
ACGAACTGCA ATCCGTACAT GCCGCCGTTG AGCTGCTCGA TCGCCTCGTC GCAGGATTTC 
AGCCATGACT ACTCGGACTA TCAGTGGTTC ATGGATTACG GGAGCTATCG CGATGGAATC 
ACCCACCAGA GCATCCTGTC GGCGCTGTCC GCCTCGTACA ACGGCATCGG CGAGCTGTCC 
TACTACGAGG ACCTCGCCAA GAACATCGAC GCCAACCTGG CCGAGGTGGA CATGGAGAGC 
TTCCGCACCG AAGACATCCA CTCGTTGCTC ACGACGCTGC CGACGATGTG CAGCGAAAGT 
GCCGAACTGC GTCGCAATCG GAACAACAAC CGGGGCGATC TGATCCTGGA CACGTGGGCC 
GAAGCTACGG GGAATCATGG GGCCGGGATC ACAACGGTGG CCGACCTGGA GAACTCGATC 
TGCAAGTCGA AATTGCTATT CTCGCCGGTG AAGGAGTCGC ACATCAGTGT GGACTCGTTG 
GACATGGATG GGTATCCGGA CGAGGATGAT ATCATACTGA CGTGCCAGGC GAACAAGAAC 
AACTACACGA TCGCGTTCGA GCAGAGCGCG CTTTACTCGG ACGAGAGTTT CTACGATGGT 
CCGGAGAATT ATGCCAAATA CAAGCATTTC AATGTGCTTC ACAACCTGGA TCACATCATC 
AGGAAGAAGG CTCAGGAAAC GGCGATGTCC GTTTCGGAAG TGGGTTACAC GACTTGGAGT 
AAGCTCAGGA AAAATGGACC GGGACAAAGG CTCCCAATGG TGGCGACAAA TCCGTTACTC 
AACCACATTT CCAGGCATGC ACCGACTTGT TTCGTACGAA AGAGCATGAG CATGCCAGAT 
CTTAAAGGAA AGTGCGATGG AACGGAAACA GATCATCATT CCGAAGAAGT AGATCACAGC 
GCAGAGGCAT CAGAACACTC GCATAAGGAT CAGCAGCACA GGCATCAGTT GAACGTATCG 
CAAGCGGCAA GCTCCGGAGC TACTTCCGGT GAGTCGTTCG GTAAGCAGAT GCGGACACTA 
CTACCGATGT ATCCGGTGCC CACCGATTCC GGCAGCGAGA GTTCCGTTCC TCTGAAGGAG 
GACTGCAACT TGGACAATAA TGAGGAGCAG CAACAGCATC AACCGTCGTT CAATTTGGTG 
AAGTTATTCA TCAAACAGAA AAGCAGTTCG ACAGACACCT GTATGGATGT TTCGTCGGGT 
TGTTGGCCGA GCGATTCTAC GAATAGTGCT TCCGGTGGTG AGAACAACAA CAACAGTAAC 
ACCAATTTGG CTGTAGCCAG TTCAAAGGAT CGTTGTCGGA AGAAAAGCAT GAACGATTCG 
GGAAAATGTT CGAACCTGGG AAGACACGAC GAGGAAGAGT TCCAGTTCGA TAGTCTGGAT 
GCACAAATCA ATAACAATAA CAACAACAAT GGGACAACGC CGATCGCCAA TGGGGACGAT 
AGGCAGGATC GGACGAGGTT GAACGATTTC AACTCCCCGG TTCATAAGAG GAACTACAAG 
GCATACAATC TGAACATCGC CAACAAGAAT CTCCCTGCGG TGAATCCGGT GGTGTACAAC 
CTAATGAACA ACAACAACAG TGGAGGAAGC AACGCCAGCA GCGGAACCAA TCTGAGCAGC 
AACAGTATTA ACAACAATAA TAATAATAAC AATAGCTTTA AGGATACCAA CTCGGATGCT 
AGTCGTACGT CGGAGAATCT GACGCAAATC TTCAACAACA GTGGCAATAA CAATGGCGAC 
GCTGCCGGTG CCAAAAGGAA GGTACCGATC GAGATGATTA CCAAGTCGAT GCAGACGTCG 
TTCATCGGGA CCAAGGAGAA GGTCCGCGTG GTACCTCCAT CATTCCTGGA GCACCTGAAC 
AAACTCGGTG ACAAACAGAA GGCCCCGGTC TTCGTTGTGT ACCCGAACTA CGTCCTCCCG 
GATTTGGGCT TCCTGAAGTC GCAAAACGAT GTCGTCATTT CGCCGCTCTC GTTCAAAGAG 
AACTTCGCCC CGCGAACGCA CCTTCGCAAA ACGCGGCCAG TTTCGATGAA CGACATCGAA 
AACCTCAAAG CCAAGGAATA CAAGCACGTG TTGGACTGGA AGTCGCTCAT AACGCTGTTA 
CCACAGGAGT ACCGAAAGCT GCTCCGTAAT ATTCCGGAGG CAAATGCATG CAGCATTGCG 
GAGGACAATG CTCCGCAGAA GCCGCTTTTC TGTATGACTC CGCCCTTGCG CCGGGGTCGA 
GGAGTTACCT GTGATTGCGT GAACATGTTC AACACGCAGA CCTACAATAG CTCCAGTTCC 
GGAGGAAGTT CTAGTCAGCC GCCCAGCTCG GGCTACCGAG GTTCGTCTAC GATGTTGACG 
GACTCCGAGA TGGATTTGTT GGGACCGAGC GGTAGCGGTG GTCTTAGCAA TAACTTGTAC 
GTTTATCAGT ATGACAGCCC AGCGGAGGTG ACATCCGCGG AAAGGCCGCC TTCGGGACGG 
GTAGCACCGA AAGGTATCCT CAGGAAAACT TCAGTCCAAA GGCCTTCATC CAAGGCCAAG 
AGAAATAGCA TGTTTGAGGA GAATCACAAA TCTGGTCAGC ACCTGGAAAA ACGACGGTCC 
CTGCAGGAAC CGTGCTACAA CTTCTCGGAA GAGAACCTCA TCTTAGAGGA CTCAATCAAC 
GAACTCCCGG ACAGTGGTCT TCGCAAAATG AACAACTCTC CAAACATGTC CCAGCATCAA 
GCCCGTCTTC CCAACACACC CAAACTACCC TCGGCTGACG ACTATCATCG GTTGAACAAA 
CTTAACGAAA TGAATGATCT CGAACTGAAC AAAGCTGTCA GCAGCAAACT GAACCGTGCT 
CAGCAACAGC AGAGCTTGGA AGATGACTCG GAAGCCCGTA CGCGTACTGA GCATTTCCTG 
TCCAATGTTC CAAAATCAGA ACTGAAACAC TATGCCGAGC TCGCTCATTT CCTGGAAGCG 
ACCACAGCAG AAAATGCCAT AGCTCAGTCC GAGGAGGTTT ACGACCGTCA GCGGTTAAGG 
CACGAAGTCA GCCGTGCGTT ATCTCAACGC AAAAATGTGT CCTTCAACCA ACCCCCATCC 
GCCGGTCCCA TCAATACCAC TCCCGTCCGG CAACAGCAAG CTCAGCTGCT GCGACCGACG 
GAGATCAAGT TTTCAACTCC TCCAAACTCG CCAAACATGT CCGTTACGAT GGCACAGAAG 
CCACCGCAGA AGCCGACTCC TTCGGCGGTG CAATCCCCTT CAAGTGCCAC CGAACGGGAG 
AAGCAGGACA AGATCCAATC GAATCGTTTC AAGCGGCTGC AGATCCAGTG GGAACTGCTG 
AGCAAGGAGG GCCAACAGTT GAAGCAGGAA TTGCAGCAAC CGCGAAGCGG TGGCAACACT 
CCTACGGGAG GAGCAATGAA ATCTCGCATT CCGCGACCCG TCAGCTATCC GACCACTCGA 
GCAAACTCGG ATCCCATGGT GAGCAAAACG TTGAAATCGC CTAGCCGAAT TGTGCCGCCC 
AAGCGGTACA ATGCCGGTTC GCTGGGCACG GTAGCAGCGA TCGGTGGCCA CCAGCAGCAG 
GCAACGACTC CGCCCACTCC GGCTCCCCGG ACGCCCAACA AGCTACTGCA TACGACTCCA 
AAGAAGGCCG TCACCGCCGG TACGCCCAGG CCAGCAACAC GCACACGGAT ACCAGTAGGT 
TCTGGTCAAA CGCAAGGTAA ATCGCTTCCC TCGTCGAATG CAGCAGTGAC AGCAGCAAAG 
GTAACGCCGA AGAAACCCTT GAACCACGCG GCACCGGCTG CCAGTAGTAG TCCCAAGGCT 
ACCATCCGGA AACCGGTCAG CAGCAGTAGT GGCCGTGACG CCGAAAAACC AAACAAAGGA 
GTCGTCAAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021697719.1
CDS	335..5149
Translation	MLLCLFVFLWKILRNMSTISATIQQEQQLELLRQQLVEHQRRKLRQLQQQKPGSGNYQKLRKLCRDKAAATKENFLLHKFLFGSEAIPENGTVNGDKGGDGSCDKMDYLSDKDLKFLRMSEYPNRQQQQGKFVLSQQNQSLLNHTHNHIHQQFGRPLLSKQNSPIRRPLACNANFWPNGSNINANNANSNNNNNAHTTTPTGALSTGVHGLFGAPGINGGGGSPGRDSLLNDENLKSNVQIVLDNINRNYHALREQILSNSNHNNLNNNCNNNNGNVNPHHLVLLQRLQSVSQQLKRTAGLSGQQPQQYQGKMAASPDSDTNCNPYMPPLSCSIASSQDFSHDYSDYQWFMDYGSYRDGITHQSILSALSASYNGIGELSYYEDLAKNIDANLAEVDMESFRTEDIHSLLTTLPTMCSESAELRRNRNNNRGDLILDTWAEATGNHGAGITTVADLENSICKSKLLFSPVKESHISVDSLDMDGYPDEDDIILTCQANKNNYTIAFEQSALYSDESFYDGPENYAKYKHFNVLHNLDHIIRKKAQETAMSVSEVGYTTWSKLRKNGPGQRLPMVATNPLLNHISRHAPTCFVRKSMSMPDLKGKCDGTETDHHSEEVDHSAEASEHSHKDQQHRHQLNVSQAASSGATSGESFGKQMRTLLPMYPVPTDSGSESSVPLKEDCNLDNNEEQQQHQPSFNLVKLFIKQKSSSTDTCMDVSSGCWPSDSTNSASGGENNNNSNTNLAVASSKDRCRKKSMNDSGKCSNLGRHDEEEFQFDSLDAQINNNNNNNGTTPIANGDDRQDRTRLNDFNSPVHKRNYKAYNLNIANKNLPAVNPVVYNLMNNNNSGGSNASSGTNLSSNSINNNNNNNNSFKDTNSDASRTSENLTQIFNNSGNNNGDAAGAKRKVPIEMITKSMQTSFIGTKEKVRVVPPSFLEHLNKLGDKQKAPVFVVYPNYVLPDLGFLKSQNDVVISPLSFKENFAPRTHLRKTRPVSMNDIENLKAKEYKHVLDWKSLITLLPQEYRKLLRNIPEANACSIAEDNAPQKPLFCMTPPLRRGRGVTCDCVNMFNTQTYNSSSSGGSSSQPPSSGYRGSSTMLTDSEMDLLGPSGSGGLSNNLYVYQYDSPAEVTSAERPPSGRVAPKGILRKTSVQRPSSKAKRNSMFEENHKSGQHLEKRRSLQEPCYNFSEENLILEDSINELPDSGLRKMNNSPNMSQHQARLPNTPKLPSADDYHRLNKLNEMNDLELNKAVSSKLNRAQQQQSLEDDSEARTRTEHFLSNVPKSELKHYAELAHFLEATTAENAIAQSEEVYDRQRLRHEVSRALSQRKNVSFNQPPSAGPINTTPVRQQQAQLLRPTEIKFSTPPNSPNMSVTMAQKPPQKPTPSAVQSPSSATEREKQDKIQSNRFKRLQIQWELLSKEGQQLKQELQQPRSGGNTPTGGAMKSRIPRPVSYPTTRANSDPMVSKTLKSPSRIVPPKRYNAGSLGTVAAIGGHQQQATTPPTPAPRTPNKLLHTTPKKAVTAGTPRPATRTRIPVGSGQTQGKSLPSSNAAVTAAKVTPKKPLNHAAPAASSSPKATIRKPVSSSSGRDAEKPNKGVVKV

Target ORF information:

RefSeq Version	XM_021842027.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021842027.1

ORF Insert Sequence:

ATGCTCCTGT GTTTGTTTGT GTTTCTGTGG AAAATCCTAC GAAATATGTC CACCATATCG 
GCAACGATAC AGCAAGAGCA GCAACTGGAA CTGCTCCGGC AGCAGTTGGT AGAACATCAG 
CGAAGAAAGT TAAGACAGTT GCAACAGCAG AAGCCGGGCA GTGGCAATTA TCAAAAACTT 
CGAAAACTGT GTCGTGATAA GGCGGCAGCT ACCAAAGAAA ACTTTCTGCT GCACAAATTT 
TTGTTCGGGT CCGAGGCGAT CCCGGAGAAC GGAACGGTGA ATGGTGATAA GGGCGGTGAT 
GGTAGCTGTG ATAAAATGGA TTACTTGAGT GATAAAGATC TTAAATTTCT TCGTATGTCA 
GAGTACCCAA ACCGTCAACA GCAGCAGGGG AAGTTTGTGT TGTCACAGCA GAATCAGTCC 
CTTCTGAATC ATACTCATAA CCATATCCAT CAGCAGTTCG GTCGTCCGCT TCTCAGCAAG 
CAAAACTCAC CCATCCGACG TCCATTGGCT TGCAACGCAA ACTTCTGGCC CAACGGCAGC 
AATATCAACG CTAACAACGC CAACAGCAAC AACAACAACA ATGCCCATAC AACTACGCCA 
ACCGGAGCGT TGTCAACTGG AGTGCACGGC TTGTTTGGGG CACCCGGAAT CAACGGGGGC 
GGCGGTAGCC CAGGCCGTGA CAGTCTGTTA AACGACGAAA ACCTCAAATC GAACGTACAG 
ATTGTGCTGG ACAATATCAA TCGAAATTAT CATGCCCTGC GGGAGCAAAT ACTGAGCAAC 
AGTAATCATA ATAATCTCAA TAACAACTGC AACAACAACA ACGGTAACGT AAATCCTCAC 
CACCTAGTGC TGCTGCAGCG GCTGCAGAGT GTGAGTCAGC AGTTGAAGAG GACGGCAGGT 
TTGTCGGGAC AACAGCCGCA GCAGTACCAG GGCAAGATGG CGGCCAGTCC CGATTCGGAC 
ACGAACTGCA ATCCGTACAT GCCGCCGTTG AGCTGCTCGA TCGCCTCGTC GCAGGATTTC 
AGCCATGACT ACTCGGACTA TCAGTGGTTC ATGGATTACG GGAGCTATCG CGATGGAATC 
ACCCACCAGA GCATCCTGTC GGCGCTGTCC GCCTCGTACA ACGGCATCGG CGAGCTGTCC 
TACTACGAGG ACCTCGCCAA GAACATCGAC GCCAACCTGG CCGAGGTGGA CATGGAGAGC 
TTCCGCACCG AAGACATCCA CTCGTTGCTC ACGACGCTGC CGACGATGTG CAGCGAAAGT 
GCCGAACTGC GTCGCAATCG GAACAACAAC CGGGGCGATC TGATCCTGGA CACGTGGGCC 
GAAGCTACGG GGAATCATGG GGCCGGGATC ACAACGGTGG CCGACCTGGA GAACTCGATC 
TGCAAGTCGA AATTGCTATT CTCGCCGGTG AAGGAGTCGC ACATCAGTGT GGACTCGTTG 
GACATGGATG GGTATCCGGA CGAGGATGAT ATCATACTGA CGTGCCAGGC GAACAAGAAC 
AACTACACGA TCGCGTTCGA GCAGAGCGCG CTTTACTCGG ACGAGAGTTT CTACGATGGT 
CCGGAGAATT ATGCCAAATA CAAGCATTTC AATGTGCTTC ACAACCTGGA TCACATCATC 
AGGAAGAAGG CTCAGGAAAC GGCGATGTCC GTTTCGGAAG TGGGTTACAC GACTTGGAGT 
AAGCTCAGGA AAAATGGACC GGGACAAAGG CTCCCAATGG TGGCGACAAA TCCGTTACTC 
AACCACATTT CCAGGCATGC ACCGACTTGT TTCGTACGAA AGAGCATGAG CATGCCAGAT 
CTTAAAGGAA AGTGCGATGG AACGGAAACA GATCATCATT CCGAAGAAGT AGATCACAGC 
GCAGAGGCAT CAGAACACTC GCATAAGGAT CAGCAGCACA GGCATCAGTT GAACGTATCG 
CAAGCGGCAA GCTCCGGAGC TACTTCCGGT GAGTCGTTCG GTAAGCAGAT GCGGACACTA 
CTACCGATGT ATCCGGTGCC CACCGATTCC GGCAGCGAGA GTTCCGTTCC TCTGAAGGAG 
GACTGCAACT TGGACAATAA TGAGGAGCAG CAACAGCATC AACCGTCGTT CAATTTGGTG 
AAGTTATTCA TCAAACAGAA AAGCAGTTCG ACAGACACCT GTATGGATGT TTCGTCGGGT 
TGTTGGCCGA GCGATTCTAC GAATAGTGCT TCCGGTGGTG AGAACAACAA CAACAGTAAC 
ACCAATTTGG CTGTAGCCAG TTCAAAGGAT CGTTGTCGGA AGAAAAGCAT GAACGATTCG 
GGAAAATGTT CGAACCTGGG AAGACACGAC GAGGAAGAGT TCCAGTTCGA TAGTCTGGAT 
GCACAAATCA ATAACAATAA CAACAACAAT GGGACAACGC CGATCGCCAA TGGGGACGAT 
AGGCAGGATC GGACGAGGTT GAACGATTTC AACTCCCCGG TTCATAAGAG GAACTACAAG 
GCATACAATC TGAACATCGC CAACAAGAAT CTCCCTGCGG TGAATCCGGT GGTGTACAAC 
CTAATGAACA ACAACAACAG TGGAGGAAGC AACGCCAGCA GCGGAACCAA TCTGAGCAGC 
AACAGTATTA ACAACAATAA TAATAATAAC AATAGCTTTA AGGATACCAA CTCGGATGCT 
AGTCGTACGT CGGAGAATCT GACGCAAATC TTCAACAACA GTGGCAATAA CAATGGCGAC 
GCTGCCGGTG CCAAAAGGAA GGTACCGATC GAGATGATTA CCAAGTCGAT GCAGACGTCG 
TTCATCGGGA CCAAGGAGAA GGTCCGCGTG GTACCTCCAT CATTCCTGGA GCACCTGAAC 
AAACTCGGTG ACAAACAGAA GGCCCCGGTC TTCGTTGTGT ACCCGAACTA CGTCCTCCCG 
GATTTGGGCT TCCTGAAGTC GCAAAACGAT GTCGTCATTT CGCCGCTCTC GTTCAAAGAG 
AACTTCGCCC CGCGAACGCA CCTTCGCAAA ACGCGGCCAG TTTCGATGAA CGACATCGAA 
AACCTCAAAG CCAAGGAATA CAAGCACGTG TTGGACTGGA AGTCGCTCAT AACGCTGTTA 
CCACAGGAGT ACCGAAAGCT GCTCCGTAAT ATTCCGGAGG CAAATGCATG CAGCATTGCG 
GAGGACAATG CTCCGCAGAA GCCGCTTTTC TGTATGACTC CGCCCTTGCG CCGGGGTCGA 
GGAGTTACCT GTGATTGCGT GAACATGTTC AACACGCAGA CCTACAATAG CTCCAGTTCC 
GGAGGAAGTT CTAGTCAGCC GCCCAGCTCG GGCTACCGAG GTTCGTCTAC GATGTTGACG 
GACTCCGAGA TGGATTTGTT GGGACCGAGC GGTAGCGGTG GTCTTAGCAA TAACTTGTAC 
GTTTATCAGT ATGACAGCCC AGCGGAGGTG ACATCCGCGG AAAGGCCGCC TTCGGGACGG 
GTAGCACCGA AAGGTATCCT CAGGAAAACT TCAGTCCAAA GGCCTTCATC CAAGGCCAAG 
AGAAATAGCA TGTTTGAGGA GAATCACAAA TCTGGTCAGC ACCTGGAAAA ACGACGGTCC 
CTGCAGGAAC CGTGCTACAA CTTCTCGGAA GAGAACCTCA TCTTAGAGGA CTCAATCAAC 
GAACTCCCGG ACAGTGGTCT TCGCAAAATG AACAACTCTC CAAACATGTC CCAGCATCAA 
GCCCGTCTTC CCAACACACC CAAACTACCC TCGGCTGACG ACTATCATCG GTTGAACAAA 
CTTAACGAAA TGAATGATCT CGAACTGAAC AAAGCTGTCA GCAGCAAACT GAACCGTGCT 
CAGCAACAGC AGAGCTTGGA AGATGACTCG GAAGCCCGTA CGCGTACTGA GCATTTCCTG 
TCCAATGTTC CAAAATCAGA ACTGAAACAC TATGCCGAGC TCGCTCATTT CCTGGAAGCG 
ACCACAGCAG AAAATGCCAT AGCTCAGTCC GAGGAGGTTT ACGACCGTCA GCGGTTAAGG 
CACGAAGTCA GCCGTGCGTT ATCTCAACGC AAAAATGTGT CCTTCAACCA ACCCCCATCC 
GCCGGTCCCA TCAATACCAC TCCCGTCCGG CAACAGCAAG CTCAGCTGCT GCGACCGACG 
GAGATCAAGT TTTCAACTCC TCCAAACTCG CCAAACATGT CCGTTACGAT GGCACAGAAG 
CCACCGCAGA AGCCGACTCC TTCGGCGGTG CAATCCCCTT CAAGTGCCAC CGAACGGGAG 
AAGCAGGACA AGATCCAATC GAATCGTTTC AAGCGGCTGC AGATCCAGTG GGAACTGCTG 
AGCAAGGAGG GCCAACAGTT GAAGCAGGAA TTGCAGCAAC CGCGAAGCGG TGGCAACACT 
CCTACGGGAG GAGCAATGAA ATCTCGCATT CCGCGACCCG TCAGCTATCC GACCACTCGA 
GCAAACTCGG ATCCCATGGT GAGCAAAACG TTGAAATCGC CTAGCCGAAT TGTGCCGCCC 
AAGCGGTACA ATGCCGGTTC GCTGGGCACG GTAGCAGCGA TCGGTGGCCA CCAGCAGCAG 
GCAACGACTC CGCCCACTCC GGCTCCCCGG ACGCCCAACA AGCTACTGCA TACGACTCCA 
AAGAAGGCCG TCACCGCCGG TACGCCCAGG CCAGCAACAC GCACACGGAT ACCAGTAGGT 
TCTGGTCAAA CGCAAGGTAA ATCGCTTCCC TCGTCGAATG CAGCAGTGAC AGCAGCAAAG 
GTAACGCCGA AGAAACCCTT GAACCACGCG GCACCGGCTG CCAGTAGTAG TCCCAAGGCT 
ACCATCCGGA AACCGGTCAG CAGCAGTAGT GGCCGTGACG CCGAAAAACC AAACAAAGGA 
GTCGTCAAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5574982 ( XM_021842032.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_021697724.1 Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X4, mRNA.

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CloneID	OAe131558
Clone ID Related Accession (Same CDS sequence)	XM_021842025.1 , XM_021842029.1 , XM_021842027.1 , XM_021842032.1
Accession Version	XM_021842032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-19
Organism	Aedes aegypti(yellow fever mosquito)
Product	uncharacterized protein LOC5574982 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035107.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aedes aegypti Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGT GTTTGTTTGT GTTTCTGTGG AAAATCCTAC GAAATATGTC CACCATATCG 
GCAACGATAC AGCAAGAGCA GCAACTGGAA CTGCTCCGGC AGCAGTTGGT AGAACATCAG 
CGAAGAAAGT TAAGACAGTT GCAACAGCAG AAGCCGGGCA GTGGCAATTA TCAAAAACTT 
CGAAAACTGT GTCGTGATAA GGCGGCAGCT ACCAAAGAAA ACTTTCTGCT GCACAAATTT 
TTGTTCGGGT CCGAGGCGAT CCCGGAGAAC GGAACGGTGA ATGGTGATAA GGGCGGTGAT 
GGTAGCTGTG ATAAAATGGA TTACTTGAGT GATAAAGATC TTAAATTTCT TCGTATGTCA 
GAGTACCCAA ACCGTCAACA GCAGCAGGGG AAGTTTGTGT TGTCACAGCA GAATCAGTCC 
CTTCTGAATC ATACTCATAA CCATATCCAT CAGCAGTTCG GTCGTCCGCT TCTCAGCAAG 
CAAAACTCAC CCATCCGACG TCCATTGGCT TGCAACGCAA ACTTCTGGCC CAACGGCAGC 
AATATCAACG CTAACAACGC CAACAGCAAC AACAACAACA ATGCCCATAC AACTACGCCA 
ACCGGAGCGT TGTCAACTGG AGTGCACGGC TTGTTTGGGG CACCCGGAAT CAACGGGGGC 
GGCGGTAGCC CAGGCCGTGA CAGTCTGTTA AACGACGAAA ACCTCAAATC GAACGTACAG 
ATTGTGCTGG ACAATATCAA TCGAAATTAT CATGCCCTGC GGGAGCAAAT ACTGAGCAAC 
AGTAATCATA ATAATCTCAA TAACAACTGC AACAACAACA ACGGTAACGT AAATCCTCAC 
CACCTAGTGC TGCTGCAGCG GCTGCAGAGT GTGAGTCAGC AGTTGAAGAG GACGGCAGGT 
TTGTCGGGAC AACAGCCGCA GCAGTACCAG GGCAAGATGG CGGCCAGTCC CGATTCGGAC 
ACGAACTGCA ATCCGTACAT GCCGCCGTTG AGCTGCTCGA TCGCCTCGTC GCAGGATTTC 
AGCCATGACT ACTCGGACTA TCAGTGGTTC ATGGATTACG GGAGCTATCG CGATGGAATC 
ACCCACCAGA GCATCCTGTC GGCGCTGTCC GCCTCGTACA ACGGCATCGG CGAGCTGTCC 
TACTACGAGG ACCTCGCCAA GAACATCGAC GCCAACCTGG CCGAGGTGGA CATGGAGAGC 
TTCCGCACCG AAGACATCCA CTCGTTGCTC ACGACGCTGC CGACGATGTG CAGCGAAAGT 
GCCGAACTGC GTCGCAATCG GAACAACAAC CGGGGCGATC TGATCCTGGA CACGTGGGCC 
GAAGCTACGG GGAATCATGG GGCCGGGATC ACAACGGTGG CCGACCTGGA GAACTCGATC 
TGCAAGTCGA AATTGCTATT CTCGCCGGTG AAGGAGTCGC ACATCAGTGT GGACTCGTTG 
GACATGGATG GGTATCCGGA CGAGGATGAT ATCATACTGA CGTGCCAGGC GAACAAGAAC 
AACTACACGA TCGCGTTCGA GCAGAGCGCG CTTTACTCGG ACGAGAGTTT CTACGATGGT 
CCGGAGAATT ATGCCAAATA CAAGCATTTC AATGTGCTTC ACAACCTGGA TCACATCATC 
AGGAAGAAGG CTCAGGAAAC GGCGATGTCC GTTTCGGAAG TGGGTTACAC GACTTGGAGT 
AAGCTCAGGA AAAATGGACC GGGACAAAGG CTCCCAATGG TGGCGACAAA TCCGTTACTC 
AACCACATTT CCAGGCATGC ACCGACTTGT TTCGTACGAA AGAGCATGAG CATGCCAGAT 
CTTAAAGGAA AGTGCGATGG AACGGAAACA GATCATCATT CCGAAGAAGT AGATCACAGC 
GCAGAGGCAT CAGAACACTC GCATAAGGAT CAGCAGCACA GGCATCAGTT GAACGTATCG 
CAAGCGGCAA GCTCCGGAGC TACTTCCGGT GAGTCGTTCG GTAAGCAGAT GCGGACACTA 
CTACCGATGT ATCCGGTGCC CACCGATTCC GGCAGCGAGA GTTCCGTTCC TCTGAAGGAG 
GACTGCAACT TGGACAATAA TGAGGAGCAG CAACAGCATC AACCGTCGTT CAATTTGGTG 
AAGTTATTCA TCAAACAGAA AAGCAGTTCG ACAGACACCT GTATGGATGT TTCGTCGGGT 
TGTTGGCCGA GCGATTCTAC GAATAGTGCT TCCGGTGGTG AGAACAACAA CAACAGTAAC 
ACCAATTTGG CTGTAGCCAG TTCAAAGGAT CGTTGTCGGA AGAAAAGCAT GAACGATTCG 
GGAAAATGTT CGAACCTGGG AAGACACGAC GAGGAAGAGT TCCAGTTCGA TAGTCTGGAT 
GCACAAATCA ATAACAATAA CAACAACAAT GGGACAACGC CGATCGCCAA TGGGGACGAT 
AGGCAGGATC GGACGAGGTT GAACGATTTC AACTCCCCGG TTCATAAGAG GAACTACAAG 
GCATACAATC TGAACATCGC CAACAAGAAT CTCCCTGCGG TGAATCCGGT GGTGTACAAC 
CTAATGAACA ACAACAACAG TGGAGGAAGC AACGCCAGCA GCGGAACCAA TCTGAGCAGC 
AACAGTATTA ACAACAATAA TAATAATAAC AATAGCTTTA AGGATACCAA CTCGGATGCT 
AGTCGTACGT CGGAGAATCT GACGCAAATC TTCAACAACA GTGGCAATAA CAATGGCGAC 
GCTGCCGGTG CCAAAAGGAA GGTACCGATC GAGATGATTA CCAAGTCGAT GCAGACGTCG 
TTCATCGGGA CCAAGGAGAA GGTCCGCGTG GTACCTCCAT CATTCCTGGA GCACCTGAAC 
AAACTCGGTG ACAAACAGAA GGCCCCGGTC TTCGTTGTGT ACCCGAACTA CGTCCTCCCG 
GATTTGGGCT TCCTGAAGTC GCAAAACGAT GTCGTCATTT CGCCGCTCTC GTTCAAAGAG 
AACTTCGCCC CGCGAACGCA CCTTCGCAAA ACGCGGCCAG TTTCGATGAA CGACATCGAA 
AACCTCAAAG CCAAGGAATA CAAGCACGTG TTGGACTGGA AGTCGCTCAT AACGCTGTTA 
CCACAGGAGT ACCGAAAGCT GCTCCGTAAT ATTCCGGAGG CAAATGCATG CAGCATTGCG 
GAGGACAATG CTCCGCAGAA GCCGCTTTTC TGTATGACTC CGCCCTTGCG CCGGGGTCGA 
GGAGTTACCT GTGATTGCGT GAACATGTTC AACACGCAGA CCTACAATAG CTCCAGTTCC 
GGAGGAAGTT CTAGTCAGCC GCCCAGCTCG GGCTACCGAG GTTCGTCTAC GATGTTGACG 
GACTCCGAGA TGGATTTGTT GGGACCGAGC GGTAGCGGTG GTCTTAGCAA TAACTTGTAC 
GTTTATCAGT ATGACAGCCC AGCGGAGGTG ACATCCGCGG AAAGGCCGCC TTCGGGACGG 
GTAGCACCGA AAGGTATCCT CAGGAAAACT TCAGTCCAAA GGCCTTCATC CAAGGCCAAG 
AGAAATAGCA TGTTTGAGGA GAATCACAAA TCTGGTCAGC ACCTGGAAAA ACGACGGTCC 
CTGCAGGAAC CGTGCTACAA CTTCTCGGAA GAGAACCTCA TCTTAGAGGA CTCAATCAAC 
GAACTCCCGG ACAGTGGTCT TCGCAAAATG AACAACTCTC CAAACATGTC CCAGCATCAA 
GCCCGTCTTC CCAACACACC CAAACTACCC TCGGCTGACG ACTATCATCG GTTGAACAAA 
CTTAACGAAA TGAATGATCT CGAACTGAAC AAAGCTGTCA GCAGCAAACT GAACCGTGCT 
CAGCAACAGC AGAGCTTGGA AGATGACTCG GAAGCCCGTA CGCGTACTGA GCATTTCCTG 
TCCAATGTTC CAAAATCAGA ACTGAAACAC TATGCCGAGC TCGCTCATTT CCTGGAAGCG 
ACCACAGCAG AAAATGCCAT AGCTCAGTCC GAGGAGGTTT ACGACCGTCA GCGGTTAAGG 
CACGAAGTCA GCCGTGCGTT ATCTCAACGC AAAAATGTGT CCTTCAACCA ACCCCCATCC 
GCCGGTCCCA TCAATACCAC TCCCGTCCGG CAACAGCAAG CTCAGCTGCT GCGACCGACG 
GAGATCAAGT TTTCAACTCC TCCAAACTCG CCAAACATGT CCGTTACGAT GGCACAGAAG 
CCACCGCAGA AGCCGACTCC TTCGGCGGTG CAATCCCCTT CAAGTGCCAC CGAACGGGAG 
AAGCAGGACA AGATCCAATC GAATCGTTTC AAGCGGCTGC AGATCCAGTG GGAACTGCTG 
AGCAAGGAGG GCCAACAGTT GAAGCAGGAA TTGCAGCAAC CGCGAAGCGG TGGCAACACT 
CCTACGGGAG GAGCAATGAA ATCTCGCATT CCGCGACCCG TCAGCTATCC GACCACTCGA 
GCAAACTCGG ATCCCATGGT GAGCAAAACG TTGAAATCGC CTAGCCGAAT TGTGCCGCCC 
AAGCGGTACA ATGCCGGTTC GCTGGGCACG GTAGCAGCGA TCGGTGGCCA CCAGCAGCAG 
GCAACGACTC CGCCCACTCC GGCTCCCCGG ACGCCCAACA AGCTACTGCA TACGACTCCA 
AAGAAGGCCG TCACCGCCGG TACGCCCAGG CCAGCAACAC GCACACGGAT ACCAGTAGGT 
TCTGGTCAAA CGCAAGGTAA ATCGCTTCCC TCGTCGAATG CAGCAGTGAC AGCAGCAAAG 
GTAACGCCGA AGAAACCCTT GAACCACGCG GCACCGGCTG CCAGTAGTAG TCCCAAGGCT 
ACCATCCGGA AACCGGTCAG CAGCAGTAGT GGCCGTGACG CCGAAAAACC AAACAAAGGA 
GTCGTCAAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021697724.1
CDS	321..5135
Translation	MLLCLFVFLWKILRNMSTISATIQQEQQLELLRQQLVEHQRRKLRQLQQQKPGSGNYQKLRKLCRDKAAATKENFLLHKFLFGSEAIPENGTVNGDKGGDGSCDKMDYLSDKDLKFLRMSEYPNRQQQQGKFVLSQQNQSLLNHTHNHIHQQFGRPLLSKQNSPIRRPLACNANFWPNGSNINANNANSNNNNNAHTTTPTGALSTGVHGLFGAPGINGGGGSPGRDSLLNDENLKSNVQIVLDNINRNYHALREQILSNSNHNNLNNNCNNNNGNVNPHHLVLLQRLQSVSQQLKRTAGLSGQQPQQYQGKMAASPDSDTNCNPYMPPLSCSIASSQDFSHDYSDYQWFMDYGSYRDGITHQSILSALSASYNGIGELSYYEDLAKNIDANLAEVDMESFRTEDIHSLLTTLPTMCSESAELRRNRNNNRGDLILDTWAEATGNHGAGITTVADLENSICKSKLLFSPVKESHISVDSLDMDGYPDEDDIILTCQANKNNYTIAFEQSALYSDESFYDGPENYAKYKHFNVLHNLDHIIRKKAQETAMSVSEVGYTTWSKLRKNGPGQRLPMVATNPLLNHISRHAPTCFVRKSMSMPDLKGKCDGTETDHHSEEVDHSAEASEHSHKDQQHRHQLNVSQAASSGATSGESFGKQMRTLLPMYPVPTDSGSESSVPLKEDCNLDNNEEQQQHQPSFNLVKLFIKQKSSSTDTCMDVSSGCWPSDSTNSASGGENNNNSNTNLAVASSKDRCRKKSMNDSGKCSNLGRHDEEEFQFDSLDAQINNNNNNNGTTPIANGDDRQDRTRLNDFNSPVHKRNYKAYNLNIANKNLPAVNPVVYNLMNNNNSGGSNASSGTNLSSNSINNNNNNNNSFKDTNSDASRTSENLTQIFNNSGNNNGDAAGAKRKVPIEMITKSMQTSFIGTKEKVRVVPPSFLEHLNKLGDKQKAPVFVVYPNYVLPDLGFLKSQNDVVISPLSFKENFAPRTHLRKTRPVSMNDIENLKAKEYKHVLDWKSLITLLPQEYRKLLRNIPEANACSIAEDNAPQKPLFCMTPPLRRGRGVTCDCVNMFNTQTYNSSSSGGSSSQPPSSGYRGSSTMLTDSEMDLLGPSGSGGLSNNLYVYQYDSPAEVTSAERPPSGRVAPKGILRKTSVQRPSSKAKRNSMFEENHKSGQHLEKRRSLQEPCYNFSEENLILEDSINELPDSGLRKMNNSPNMSQHQARLPNTPKLPSADDYHRLNKLNEMNDLELNKAVSSKLNRAQQQQSLEDDSEARTRTEHFLSNVPKSELKHYAELAHFLEATTAENAIAQSEEVYDRQRLRHEVSRALSQRKNVSFNQPPSAGPINTTPVRQQQAQLLRPTEIKFSTPPNSPNMSVTMAQKPPQKPTPSAVQSPSSATEREKQDKIQSNRFKRLQIQWELLSKEGQQLKQELQQPRSGGNTPTGGAMKSRIPRPVSYPTTRANSDPMVSKTLKSPSRIVPPKRYNAGSLGTVAAIGGHQQQATTPPTPAPRTPNKLLHTTPKKAVTAGTPRPATRTRIPVGSGQTQGKSLPSSNAAVTAAKVTPKKPLNHAAPAASSSPKATIRKPVSSSSGRDAEKPNKGVVKV

Target ORF information:

RefSeq Version	XM_021842032.1
Organism	Aedes aegypti(yellow fever mosquito)
Definition	Aedes aegypti uncharacterized LOC5574982 (LOC5574982), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021842032.1

ORF Insert Sequence:

ATGCTCCTGT GTTTGTTTGT GTTTCTGTGG AAAATCCTAC GAAATATGTC CACCATATCG 
GCAACGATAC AGCAAGAGCA GCAACTGGAA CTGCTCCGGC AGCAGTTGGT AGAACATCAG 
CGAAGAAAGT TAAGACAGTT GCAACAGCAG AAGCCGGGCA GTGGCAATTA TCAAAAACTT 
CGAAAACTGT GTCGTGATAA GGCGGCAGCT ACCAAAGAAA ACTTTCTGCT GCACAAATTT 
TTGTTCGGGT CCGAGGCGAT CCCGGAGAAC GGAACGGTGA ATGGTGATAA GGGCGGTGAT 
GGTAGCTGTG ATAAAATGGA TTACTTGAGT GATAAAGATC TTAAATTTCT TCGTATGTCA 
GAGTACCCAA ACCGTCAACA GCAGCAGGGG AAGTTTGTGT TGTCACAGCA GAATCAGTCC 
CTTCTGAATC ATACTCATAA CCATATCCAT CAGCAGTTCG GTCGTCCGCT TCTCAGCAAG 
CAAAACTCAC CCATCCGACG TCCATTGGCT TGCAACGCAA ACTTCTGGCC CAACGGCAGC 
AATATCAACG CTAACAACGC CAACAGCAAC AACAACAACA ATGCCCATAC AACTACGCCA 
ACCGGAGCGT TGTCAACTGG AGTGCACGGC TTGTTTGGGG CACCCGGAAT CAACGGGGGC 
GGCGGTAGCC CAGGCCGTGA CAGTCTGTTA AACGACGAAA ACCTCAAATC GAACGTACAG 
ATTGTGCTGG ACAATATCAA TCGAAATTAT CATGCCCTGC GGGAGCAAAT ACTGAGCAAC 
AGTAATCATA ATAATCTCAA TAACAACTGC AACAACAACA ACGGTAACGT AAATCCTCAC 
CACCTAGTGC TGCTGCAGCG GCTGCAGAGT GTGAGTCAGC AGTTGAAGAG GACGGCAGGT 
TTGTCGGGAC AACAGCCGCA GCAGTACCAG GGCAAGATGG CGGCCAGTCC CGATTCGGAC 
ACGAACTGCA ATCCGTACAT GCCGCCGTTG AGCTGCTCGA TCGCCTCGTC GCAGGATTTC 
AGCCATGACT ACTCGGACTA TCAGTGGTTC ATGGATTACG GGAGCTATCG CGATGGAATC 
ACCCACCAGA GCATCCTGTC GGCGCTGTCC GCCTCGTACA ACGGCATCGG CGAGCTGTCC 
TACTACGAGG ACCTCGCCAA GAACATCGAC GCCAACCTGG CCGAGGTGGA CATGGAGAGC 
TTCCGCACCG AAGACATCCA CTCGTTGCTC ACGACGCTGC CGACGATGTG CAGCGAAAGT 
GCCGAACTGC GTCGCAATCG GAACAACAAC CGGGGCGATC TGATCCTGGA CACGTGGGCC 
GAAGCTACGG GGAATCATGG GGCCGGGATC ACAACGGTGG CCGACCTGGA GAACTCGATC 
TGCAAGTCGA AATTGCTATT CTCGCCGGTG AAGGAGTCGC ACATCAGTGT GGACTCGTTG 
GACATGGATG GGTATCCGGA CGAGGATGAT ATCATACTGA CGTGCCAGGC GAACAAGAAC 
AACTACACGA TCGCGTTCGA GCAGAGCGCG CTTTACTCGG ACGAGAGTTT CTACGATGGT 
CCGGAGAATT ATGCCAAATA CAAGCATTTC AATGTGCTTC ACAACCTGGA TCACATCATC 
AGGAAGAAGG CTCAGGAAAC GGCGATGTCC GTTTCGGAAG TGGGTTACAC GACTTGGAGT 
AAGCTCAGGA AAAATGGACC GGGACAAAGG CTCCCAATGG TGGCGACAAA TCCGTTACTC 
AACCACATTT CCAGGCATGC ACCGACTTGT TTCGTACGAA AGAGCATGAG CATGCCAGAT 
CTTAAAGGAA AGTGCGATGG AACGGAAACA GATCATCATT CCGAAGAAGT AGATCACAGC 
GCAGAGGCAT CAGAACACTC GCATAAGGAT CAGCAGCACA GGCATCAGTT GAACGTATCG 
CAAGCGGCAA GCTCCGGAGC TACTTCCGGT GAGTCGTTCG GTAAGCAGAT GCGGACACTA 
CTACCGATGT ATCCGGTGCC CACCGATTCC GGCAGCGAGA GTTCCGTTCC TCTGAAGGAG 
GACTGCAACT TGGACAATAA TGAGGAGCAG CAACAGCATC AACCGTCGTT CAATTTGGTG 
AAGTTATTCA TCAAACAGAA AAGCAGTTCG ACAGACACCT GTATGGATGT TTCGTCGGGT 
TGTTGGCCGA GCGATTCTAC GAATAGTGCT TCCGGTGGTG AGAACAACAA CAACAGTAAC 
ACCAATTTGG CTGTAGCCAG TTCAAAGGAT CGTTGTCGGA AGAAAAGCAT GAACGATTCG 
GGAAAATGTT CGAACCTGGG AAGACACGAC GAGGAAGAGT TCCAGTTCGA TAGTCTGGAT 
GCACAAATCA ATAACAATAA CAACAACAAT GGGACAACGC CGATCGCCAA TGGGGACGAT 
AGGCAGGATC GGACGAGGTT GAACGATTTC AACTCCCCGG TTCATAAGAG GAACTACAAG 
GCATACAATC TGAACATCGC CAACAAGAAT CTCCCTGCGG TGAATCCGGT GGTGTACAAC 
CTAATGAACA ACAACAACAG TGGAGGAAGC AACGCCAGCA GCGGAACCAA TCTGAGCAGC 
AACAGTATTA ACAACAATAA TAATAATAAC AATAGCTTTA AGGATACCAA CTCGGATGCT 
AGTCGTACGT CGGAGAATCT GACGCAAATC TTCAACAACA GTGGCAATAA CAATGGCGAC 
GCTGCCGGTG CCAAAAGGAA GGTACCGATC GAGATGATTA CCAAGTCGAT GCAGACGTCG 
TTCATCGGGA CCAAGGAGAA GGTCCGCGTG GTACCTCCAT CATTCCTGGA GCACCTGAAC 
AAACTCGGTG ACAAACAGAA GGCCCCGGTC TTCGTTGTGT ACCCGAACTA CGTCCTCCCG 
GATTTGGGCT TCCTGAAGTC GCAAAACGAT GTCGTCATTT CGCCGCTCTC GTTCAAAGAG 
AACTTCGCCC CGCGAACGCA CCTTCGCAAA ACGCGGCCAG TTTCGATGAA CGACATCGAA 
AACCTCAAAG CCAAGGAATA CAAGCACGTG TTGGACTGGA AGTCGCTCAT AACGCTGTTA 
CCACAGGAGT ACCGAAAGCT GCTCCGTAAT ATTCCGGAGG CAAATGCATG CAGCATTGCG 
GAGGACAATG CTCCGCAGAA GCCGCTTTTC TGTATGACTC CGCCCTTGCG CCGGGGTCGA 
GGAGTTACCT GTGATTGCGT GAACATGTTC AACACGCAGA CCTACAATAG CTCCAGTTCC 
GGAGGAAGTT CTAGTCAGCC GCCCAGCTCG GGCTACCGAG GTTCGTCTAC GATGTTGACG 
GACTCCGAGA TGGATTTGTT GGGACCGAGC GGTAGCGGTG GTCTTAGCAA TAACTTGTAC 
GTTTATCAGT ATGACAGCCC AGCGGAGGTG ACATCCGCGG AAAGGCCGCC TTCGGGACGG 
GTAGCACCGA AAGGTATCCT CAGGAAAACT TCAGTCCAAA GGCCTTCATC CAAGGCCAAG 
AGAAATAGCA TGTTTGAGGA GAATCACAAA TCTGGTCAGC ACCTGGAAAA ACGACGGTCC 
CTGCAGGAAC CGTGCTACAA CTTCTCGGAA GAGAACCTCA TCTTAGAGGA CTCAATCAAC 
GAACTCCCGG ACAGTGGTCT TCGCAAAATG AACAACTCTC CAAACATGTC CCAGCATCAA 
GCCCGTCTTC CCAACACACC CAAACTACCC TCGGCTGACG ACTATCATCG GTTGAACAAA 
CTTAACGAAA TGAATGATCT CGAACTGAAC AAAGCTGTCA GCAGCAAACT GAACCGTGCT 
CAGCAACAGC AGAGCTTGGA AGATGACTCG GAAGCCCGTA CGCGTACTGA GCATTTCCTG 
TCCAATGTTC CAAAATCAGA ACTGAAACAC TATGCCGAGC TCGCTCATTT CCTGGAAGCG 
ACCACAGCAG AAAATGCCAT AGCTCAGTCC GAGGAGGTTT ACGACCGTCA GCGGTTAAGG 
CACGAAGTCA GCCGTGCGTT ATCTCAACGC AAAAATGTGT CCTTCAACCA ACCCCCATCC 
GCCGGTCCCA TCAATACCAC TCCCGTCCGG CAACAGCAAG CTCAGCTGCT GCGACCGACG 
GAGATCAAGT TTTCAACTCC TCCAAACTCG CCAAACATGT CCGTTACGAT GGCACAGAAG 
CCACCGCAGA AGCCGACTCC TTCGGCGGTG CAATCCCCTT CAAGTGCCAC CGAACGGGAG 
AAGCAGGACA AGATCCAATC GAATCGTTTC AAGCGGCTGC AGATCCAGTG GGAACTGCTG 
AGCAAGGAGG GCCAACAGTT GAAGCAGGAA TTGCAGCAAC CGCGAAGCGG TGGCAACACT 
CCTACGGGAG GAGCAATGAA ATCTCGCATT CCGCGACCCG TCAGCTATCC GACCACTCGA 
GCAAACTCGG ATCCCATGGT GAGCAAAACG TTGAAATCGC CTAGCCGAAT TGTGCCGCCC 
AAGCGGTACA ATGCCGGTTC GCTGGGCACG GTAGCAGCGA TCGGTGGCCA CCAGCAGCAG 
GCAACGACTC CGCCCACTCC GGCTCCCCGG ACGCCCAACA AGCTACTGCA TACGACTCCA 
AAGAAGGCCG TCACCGCCGG TACGCCCAGG CCAGCAACAC GCACACGGAT ACCAGTAGGT 
TCTGGTCAAA CGCAAGGTAA ATCGCTTCCC TCGTCGAATG CAGCAGTGAC AGCAGCAAAG 
GTAACGCCGA AGAAACCCTT GAACCACGCG GCACCGGCTG CCAGTAGTAG TCCCAAGGCT 
ACCATCCGGA AACCGGTCAG CAGCAGTAGT GGCCGTGACG CCGAAAAACC AAACAAAGGA 
GTCGTCAAAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC5574982 ( XM_001655405.1 ) cDNA ORF clone, Aedes aegypti(yellow fever mosquito) -> XP_001655455.1 Aedes aegypti AAEL002498-RA partial mRNA.

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CloneID	OAe10044
Clone ID Related Accession (Same CDS sequence)	XM_001655405.1
Accession Version	XM_001655405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-04
Organism	Aedes aegypti(Yellow fever mosquito)
Product	AAEL002498-PA
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001811340). COMPLETENESS: incomplete on both ends.

ATGTCAGAGT ACCCAAACCG TCAACAGCAG CAGGGGAAGT TTGTGTTGTC ACAGCAGAAT 
CAGTCCCTTC TGAATCATAC TCATAACCAT ATCCATCAGC AGTCCGGTCG TCCGCTTCTC 
AGCAAGCAGA ACTTACCCAT CCGACGTCCA TTGGCTTGCA ACGCAAACTT CTGGCCCAAC 
GGCAGCAATA TCAACGCCAA CGCCAACAGC AACATCAACA ACAATGTCCA TACAACTACG 
CCAACCGGAG CGTTGTCAAC TGGAGTGCAC GGCTTGTTTG GGGCACCCGG AATCAACGGG 
GGCGGCGGTA GCCCAGGCCG TGACAGTCTG TTAAACGACG AAAACCTCAA ATCGAACGTA 
CAGATTGTGC TGGACAATAT CAATCGAAAT TATCATGCCC TGCGGGAGCA AATACTGAGC 
AACAGTAATC ATAATAATCT CAATAACAAC TGCAACAACA ACAACGGTAA CGTAAATCCT 
CACCACCTAG TGCTGCTGCA GCGGCTGCAG AGCGTTAGTC AGCAGTTGAA GAGGACGGCA 
GGTTTGTCGG GACAACAGCC GCAGCAGTAC CAGGGCAAGA TGGCGGCCAC CAGTCCCGAT 
TCGGACACGA ACTGCAACCC GTACATGCCG CCGTTGAGCT GCTCGATCGC CTCGTCGCAG 
GACTTCAGCC ATGACTACTC GGACTATCAG TGGTTCATGG ATTACGGCTA TCGCGATGGC 
ATCACCCACC AGAGCATCCT GTCGGCGCTG TCCGCCTCGT ACAACGGTAT CGGCGAGCTG 
TCCTACTACG AGGACCTCGC CAAGAACATC GACGCCAACC TGGCTGAGGT GGACATGGAG 
AGCTTCCGCA CCGAAGACAT CCACTCGTTG CTCACGACGC TGCCGACGAT GTGCAGCGAA 
AGTGCCGAAC TGCGTCGCAA TCGGAACAAC AACCGGGGCG ATCTGATCCT GGACACGTGG 
GCCGAAGCTA CGGGGAATCA TGGGGCCGGG ATCACAACGG TGGCTGACCT GGAGAACTCG 
ATCTGCAAGT CGAAATTGCT ATTCTCGCCG GTGAAGGAGT CGCACATCAG TGTGGACTCG 
TTGGACATGG ATGGGTATCC GGACGAGGAT GATATCATAC TGACGTGCCA GGCGAACAAG 
AACAACTACA CGATCGCGTT TGAGCAGAGC GCACTCTACT CGGACGAGAG TTTCTACGAT 
GGTCCGGAGA ATTATGCCAA ATACAAGCAT TTCAATGTGC TCCACAACCT GGATCACATC 
ATCAGGAAGA AGGCTCAGGA AACGGCGATG TCCGTTTCGG AAGTGGGCTA CACGACTTGG 
AGTAAGCTCA GGAAAAATGG ACCGGGACAA AGGCTCCCAA TGGTGGCGAC AAATCCGTTA 
CTCAACCACA TTTCCAGACA TGCACCGACT TGTTTCGTAC GAAAGAGCAT GAGCATGCCA 
GATCTTAAAG GAAAGTGCGA TGGAACGGAA ACAGATCATC ATTCCGATGA AGTAGATCAC 
AGTGCAGAGG CATCAGAACA TTCGCATAAG GATCAGCAGC ACAGGCATCA GTTGAACGTA 
TCGCAAGCGG CAAGCTCCGG GGCTACTTCC GGGGAGTCGT TCGGTAAGCA GATGCGGACA 
CTACTACCGA TGTATCCGGT GCCCACCGAT TCCGGCAGCG AGAGTTCCGT TCCTCTGAAG 
GAGGACTGCA ACTTGGACAA TAATGACGAG CAGCAACAGC ATCAACCGTC GTTCAATTTG 
GTGAAGTTAT TCATCAAACA GAAGAGCAGT TCGACAGACA CCTGTATGGA TGTTTCGTCG 
GGCTGTTGGC CGAGCGATTC CACGAATAGT GCTTCCGGTG GGGAGAACAA CAACAACAGT 
AACACCAATT TGGCCGTGGC CAGTTCGAAG GATCGTTGTC GGAAGAAGAG CATGAACGAT 
TCGGGCAAAT GTTCGAACCT GGGAAGACAC GACGAGGAAG AGTTCCAGTT CGATAGTCTG 
GATGCACAAA TCAATAACAA TAATAATAAC AATGGGACAA CGCCGATCGC CAATGGGGAC 
GATAGGCAGG ACCGGACGAG GTTGAACGAT TTCAACTCCC CGGTTCATAA GCGGAACTAC 
AAGGCATACA ATCTGAACAT CGCCAACAAG AACCTCCCGG CGGATACCAA CTCGGATGCT 
AGTCGTACGT CGGAGAATCT GACGCAAATC TTCAACAACA GTGGCAATAA CAATGGCGAC 
GCTGCCGGTG CCAAGAGGAA GGTACCGATC GAGATGATTA CCAAGTCGAT GCAGACGTCG 
TTCATCGGGA CCAAGGAGAA GGTCCGCGTG GTACCTCCAT CATTCCTGGA GCACCTGAAC 
AAACTCGGTG ACAAACAGAA GGCCCCGGTC TTCGTTGTGT ACCCGAACTA CGTCCTCCCG 
GATTTGGGCT TCCTGAAGTC GCAAAACGAT GTCGTCATTT CGCCGCTCTC GTTCAAGGAG 
AACTTCGCCC CGCGAACGCA CCTTCGCAAA ACGCGGCCAG TTTCGATGAA CGACATCGAA 
AACCTCAAAG CCAAGGAATA CAAGCACGTG TTGGACTGGA AGTCGCTCAT AACGCTGTTA 
CCACAGGAGT ACCGAAAGCT GCTCCGTAAT ATTCCGGAGG CAAATGCATG CAGCATTGCG 
GAGGACAATG CTCCGCAGAA GCCGCTTTTC TGTATGACTC CTCCCTTGCG CCGAGGTCGA 
GGAGTTACCT GTGATTGCGT GAACATGTTC AACACGCAGA CCTACAATAG CTCCAGTTCC 
GGAGGAAGTT CTAGTCAGCC GCCCAGTTCG GGCTACCGAG GTTCGTCTAC GATGTTGACG 
GACTCCGAGA TGGATTTGTT GGGGCCGAGC GGTAGCGGTG GTCTTAGCAA TAACTTGTAC 
GTTTATCAGT ATGACAGCCC AGCGGAGGTG ACATCCGCGG AAAGGCCGCC TTCGGGACGG 
GTAGCACCGA AAGGTATCCT CAGGAAAACT TCAGTCCAAA GGCCTTCATC CAAGGCCAAG 
AGAAATAGCA TGTTTGAGGA GAATCACAAA TCTGGTCAGC ACCTGGAAAA ACGACGGTCC 
CTGCAGGAAC CGTGCTACAA CTTCTCGGAA GAGAACCTCA TCTTAGAGGA CTCAATCAAC 
GAACTCCCGG ACAGTGGTCT TCGCAAAATG AACAACTCTC CAAACATGTC CCAGCATCAA 
GCCCGTCTTC CCAACACACC CAAACTACCC TCGGCTGACG ACTATCATCG GTTGAACAAA 
CTTAACGAAA TGAATGATCT CGAACTGAAC AAAGCTGTCA GCAGCAAACT GAACCGTGCT 
CAGCAACAGC AGAGCTTGGA AGATGACTCG GAAGCCCGTA CGCGTACTGA GCATTTCCTG 
TCCAATGTTC CAAAATCAGA ACTGAAACAC TATGCCGAGC TCGCTCATTT CCTGGAAGCG 
ACCACAGCAG AAAATGCCAT AGCTCAGTCC GAGGAGGTTT ACGACCGTCA GCGGTTAAGG 
CACGAAGTCA GCCGTGCGTT ATCTCAACGC AAAAATGTGT CCTTCAACCA ACCCCCAACC 
GCCGGTCCCA TCAATACCAC TCCCGTCCGG CAACAGCAAG CTCAGCTGCT GCGACCGACG 
GAGATCAAGT TTTCAACTCC TCCAAACTCG CCAAACATGT CCGTTACCAT GGCACAGAAG 
CCACCGCAGA AGCCGACTCC TTCGGCGGTG CAGTCCCCTT CGAGTGCCAC CGAGCGAGAG 
AAGCAGGACA AGATCCAATC GAACCGTTTC AAGCGGTTGC AGATCCAATG GGAACTGCTG 
AGCAAGGAGG GCCAACAGTT GAAGCAGGAA TTGCAGCAAC CGCGCAGCGG TGGCAACACT 
CCCACGGGAG GTGCAATGAA ATCTCGCATT CCACGACCCG TCAGCTATCC GACCACTCGA 
GCAAACTCGG ATCCCATGGT GAGCAAAACG TTGAAATCGC CGAGCCGAAT TGTGCCGCCC 
AAGCGGTACA ATGCCGGTTC GCTGGGCACG GTAGCAGCGA TCGGTGGCCA CCAGCAGCAG 
GCAACGACTC CGCCCACTCC GGCTCCCCGG ACGCCCAACA AGCTACTGCA TACGACTCCA 
AAGAAGGCCG CCACCGCCGG TACGCCCAGG CCAGCAACAC GCACACGGAT ACCAGTAGGT 
TCTGGTCAAA CGCAAGGTAA ATCGCTTCCC TCGTCGAATG CAGCAGTAAA GGTAATGCCG 
AAGAAACCCT TGAACCACGC GGCCCCGGCT GCCAGTAGTA GTCCCAAGGC TACCATCCGG 
AAACCGGTCA GCAGCAGTAG TGGCCGTGTC GCCGAAAAAC CAAACAAAGG AGTCGTCAAA 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001655455.1
CDS	1..4326
Translation	MSEYPNRQQQQGKFVLSQQNQSLLNHTHNHIHQQSGRPLLSKQNLPIRRPLACNANFWPNGSNINANANSNINNNVHTTTPTGALSTGVHGLFGAPGINGGGGSPGRDSLLNDENLKSNVQIVLDNINRNYHALREQILSNSNHNNLNNNCNNNNGNVNPHHLVLLQRLQSVSQQLKRTAGLSGQQPQQYQGKMAATSPDSDTNCNPYMPPLSCSIASSQDFSHDYSDYQWFMDYGYRDGITHQSILSALSASYNGIGELSYYEDLAKNIDANLAEVDMESFRTEDIHSLLTTLPTMCSESAELRRNRNNNRGDLILDTWAEATGNHGAGITTVADLENSICKSKLLFSPVKESHISVDSLDMDGYPDEDDIILTCQANKNNYTIAFEQSALYSDESFYDGPENYAKYKHFNVLHNLDHIIRKKAQETAMSVSEVGYTTWSKLRKNGPGQRLPMVATNPLLNHISRHAPTCFVRKSMSMPDLKGKCDGTETDHHSDEVDHSAEASEHSHKDQQHRHQLNVSQAASSGATSGESFGKQMRTLLPMYPVPTDSGSESSVPLKEDCNLDNNDEQQQHQPSFNLVKLFIKQKSSSTDTCMDVSSGCWPSDSTNSASGGENNNNSNTNLAVASSKDRCRKKSMNDSGKCSNLGRHDEEEFQFDSLDAQINNNNNNNGTTPIANGDDRQDRTRLNDFNSPVHKRNYKAYNLNIANKNLPADTNSDASRTSENLTQIFNNSGNNNGDAAGAKRKVPIEMITKSMQTSFIGTKEKVRVVPPSFLEHLNKLGDKQKAPVFVVYPNYVLPDLGFLKSQNDVVISPLSFKENFAPRTHLRKTRPVSMNDIENLKAKEYKHVLDWKSLITLLPQEYRKLLRNIPEANACSIAEDNAPQKPLFCMTPPLRRGRGVTCDCVNMFNTQTYNSSSSGGSSSQPPSSGYRGSSTMLTDSEMDLLGPSGSGGLSNNLYVYQYDSPAEVTSAERPPSGRVAPKGILRKTSVQRPSSKAKRNSMFEENHKSGQHLEKRRSLQEPCYNFSEENLILEDSINELPDSGLRKMNNSPNMSQHQARLPNTPKLPSADDYHRLNKLNEMNDLELNKAVSSKLNRAQQQQSLEDDSEARTRTEHFLSNVPKSELKHYAELAHFLEATTAENAIAQSEEVYDRQRLRHEVSRALSQRKNVSFNQPPTAGPINTTPVRQQQAQLLRPTEIKFSTPPNSPNMSVTMAQKPPQKPTPSAVQSPSSATEREKQDKIQSNRFKRLQIQWELLSKEGQQLKQELQQPRSGGNTPTGGAMKSRIPRPVSYPTTRANSDPMVSKTLKSPSRIVPPKRYNAGSLGTVAAIGGHQQQATTPPTPAPRTPNKLLHTTPKKAATAGTPRPATRTRIPVGSGQTQGKSLPSSNAAVKVMPKKPLNHAAPAASSSPKATIRKPVSSSSGRVAEKPNKGVVKV

Target ORF information:

RefSeq Version	XM_001655405.1
Organism	Aedes aegypti(Yellow fever mosquito)
Definition	Aedes aegypti AAEL002498-RA partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001655405.1

ORF Insert Sequence:

ATGTCAGAGT ACCCAAACCG TCAACAGCAG CAGGGGAAGT TTGTGTTGTC ACAGCAGAAT 
CAGTCCCTTC TGAATCATAC TCATAACCAT ATCCATCAGC AGTCCGGTCG TCCGCTTCTC 
AGCAAGCAGA ACTTACCCAT CCGACGTCCA TTGGCTTGCA ACGCAAACTT CTGGCCCAAC 
GGCAGCAATA TCAACGCCAA CGCCAACAGC AACATCAACA ACAATGTCCA TACAACTACG 
CCAACCGGAG CGTTGTCAAC TGGAGTGCAC GGCTTGTTTG GGGCACCCGG AATCAACGGG 
GGCGGCGGTA GCCCAGGCCG TGACAGTCTG TTAAACGACG AAAACCTCAA ATCGAACGTA 
CAGATTGTGC TGGACAATAT CAATCGAAAT TATCATGCCC TGCGGGAGCA AATACTGAGC 
AACAGTAATC ATAATAATCT CAATAACAAC TGCAACAACA ACAACGGTAA CGTAAATCCT 
CACCACCTAG TGCTGCTGCA GCGGCTGCAG AGCGTTAGTC AGCAGTTGAA GAGGACGGCA 
GGTTTGTCGG GACAACAGCC GCAGCAGTAC CAGGGCAAGA TGGCGGCCAC CAGTCCCGAT 
TCGGACACGA ACTGCAACCC GTACATGCCG CCGTTGAGCT GCTCGATCGC CTCGTCGCAG 
GACTTCAGCC ATGACTACTC GGACTATCAG TGGTTCATGG ATTACGGCTA TCGCGATGGC 
ATCACCCACC AGAGCATCCT GTCGGCGCTG TCCGCCTCGT ACAACGGTAT CGGCGAGCTG 
TCCTACTACG AGGACCTCGC CAAGAACATC GACGCCAACC TGGCTGAGGT GGACATGGAG 
AGCTTCCGCA CCGAAGACAT CCACTCGTTG CTCACGACGC TGCCGACGAT GTGCAGCGAA 
AGTGCCGAAC TGCGTCGCAA TCGGAACAAC AACCGGGGCG ATCTGATCCT GGACACGTGG 
GCCGAAGCTA CGGGGAATCA TGGGGCCGGG ATCACAACGG TGGCTGACCT GGAGAACTCG 
ATCTGCAAGT CGAAATTGCT ATTCTCGCCG GTGAAGGAGT CGCACATCAG TGTGGACTCG 
TTGGACATGG ATGGGTATCC GGACGAGGAT GATATCATAC TGACGTGCCA GGCGAACAAG 
AACAACTACA CGATCGCGTT TGAGCAGAGC GCACTCTACT CGGACGAGAG TTTCTACGAT 
GGTCCGGAGA ATTATGCCAA ATACAAGCAT TTCAATGTGC TCCACAACCT GGATCACATC 
ATCAGGAAGA AGGCTCAGGA AACGGCGATG TCCGTTTCGG AAGTGGGCTA CACGACTTGG 
AGTAAGCTCA GGAAAAATGG ACCGGGACAA AGGCTCCCAA TGGTGGCGAC AAATCCGTTA 
CTCAACCACA TTTCCAGACA TGCACCGACT TGTTTCGTAC GAAAGAGCAT GAGCATGCCA 
GATCTTAAAG GAAAGTGCGA TGGAACGGAA ACAGATCATC ATTCCGATGA AGTAGATCAC 
AGTGCAGAGG CATCAGAACA TTCGCATAAG GATCAGCAGC ACAGGCATCA GTTGAACGTA 
TCGCAAGCGG CAAGCTCCGG GGCTACTTCC GGGGAGTCGT TCGGTAAGCA GATGCGGACA 
CTACTACCGA TGTATCCGGT GCCCACCGAT TCCGGCAGCG AGAGTTCCGT TCCTCTGAAG 
GAGGACTGCA ACTTGGACAA TAATGACGAG CAGCAACAGC ATCAACCGTC GTTCAATTTG 
GTGAAGTTAT TCATCAAACA GAAGAGCAGT TCGACAGACA CCTGTATGGA TGTTTCGTCG 
GGCTGTTGGC CGAGCGATTC CACGAATAGT GCTTCCGGTG GGGAGAACAA CAACAACAGT 
AACACCAATT TGGCCGTGGC CAGTTCGAAG GATCGTTGTC GGAAGAAGAG CATGAACGAT 
TCGGGCAAAT GTTCGAACCT GGGAAGACAC GACGAGGAAG AGTTCCAGTT CGATAGTCTG 
GATGCACAAA TCAATAACAA TAATAATAAC AATGGGACAA CGCCGATCGC CAATGGGGAC 
GATAGGCAGG ACCGGACGAG GTTGAACGAT TTCAACTCCC CGGTTCATAA GCGGAACTAC 
AAGGCATACA ATCTGAACAT CGCCAACAAG AACCTCCCGG CGGATACCAA CTCGGATGCT 
AGTCGTACGT CGGAGAATCT GACGCAAATC TTCAACAACA GTGGCAATAA CAATGGCGAC 
GCTGCCGGTG CCAAGAGGAA GGTACCGATC GAGATGATTA CCAAGTCGAT GCAGACGTCG 
TTCATCGGGA CCAAGGAGAA GGTCCGCGTG GTACCTCCAT CATTCCTGGA GCACCTGAAC 
AAACTCGGTG ACAAACAGAA GGCCCCGGTC TTCGTTGTGT ACCCGAACTA CGTCCTCCCG 
GATTTGGGCT TCCTGAAGTC GCAAAACGAT GTCGTCATTT CGCCGCTCTC GTTCAAGGAG 
AACTTCGCCC CGCGAACGCA CCTTCGCAAA ACGCGGCCAG TTTCGATGAA CGACATCGAA 
AACCTCAAAG CCAAGGAATA CAAGCACGTG TTGGACTGGA AGTCGCTCAT AACGCTGTTA 
CCACAGGAGT ACCGAAAGCT GCTCCGTAAT ATTCCGGAGG CAAATGCATG CAGCATTGCG 
GAGGACAATG CTCCGCAGAA GCCGCTTTTC TGTATGACTC CTCCCTTGCG CCGAGGTCGA 
GGAGTTACCT GTGATTGCGT GAACATGTTC AACACGCAGA CCTACAATAG CTCCAGTTCC 
GGAGGAAGTT CTAGTCAGCC GCCCAGTTCG GGCTACCGAG GTTCGTCTAC GATGTTGACG 
GACTCCGAGA TGGATTTGTT GGGGCCGAGC GGTAGCGGTG GTCTTAGCAA TAACTTGTAC 
GTTTATCAGT ATGACAGCCC AGCGGAGGTG ACATCCGCGG AAAGGCCGCC TTCGGGACGG 
GTAGCACCGA AAGGTATCCT CAGGAAAACT TCAGTCCAAA GGCCTTCATC CAAGGCCAAG 
AGAAATAGCA TGTTTGAGGA GAATCACAAA TCTGGTCAGC ACCTGGAAAA ACGACGGTCC 
CTGCAGGAAC CGTGCTACAA CTTCTCGGAA GAGAACCTCA TCTTAGAGGA CTCAATCAAC 
GAACTCCCGG ACAGTGGTCT TCGCAAAATG AACAACTCTC CAAACATGTC CCAGCATCAA 
GCCCGTCTTC CCAACACACC CAAACTACCC TCGGCTGACG ACTATCATCG GTTGAACAAA 
CTTAACGAAA TGAATGATCT CGAACTGAAC AAAGCTGTCA GCAGCAAACT GAACCGTGCT 
CAGCAACAGC AGAGCTTGGA AGATGACTCG GAAGCCCGTA CGCGTACTGA GCATTTCCTG 
TCCAATGTTC CAAAATCAGA ACTGAAACAC TATGCCGAGC TCGCTCATTT CCTGGAAGCG 
ACCACAGCAG AAAATGCCAT AGCTCAGTCC GAGGAGGTTT ACGACCGTCA GCGGTTAAGG 
CACGAAGTCA GCCGTGCGTT ATCTCAACGC AAAAATGTGT CCTTCAACCA ACCCCCAACC 
GCCGGTCCCA TCAATACCAC TCCCGTCCGG CAACAGCAAG CTCAGCTGCT GCGACCGACG 
GAGATCAAGT TTTCAACTCC TCCAAACTCG CCAAACATGT CCGTTACCAT GGCACAGAAG 
CCACCGCAGA AGCCGACTCC TTCGGCGGTG CAGTCCCCTT CGAGTGCCAC CGAGCGAGAG 
AAGCAGGACA AGATCCAATC GAACCGTTTC AAGCGGTTGC AGATCCAATG GGAACTGCTG 
AGCAAGGAGG GCCAACAGTT GAAGCAGGAA TTGCAGCAAC CGCGCAGCGG TGGCAACACT 
CCCACGGGAG GTGCAATGAA ATCTCGCATT CCACGACCCG TCAGCTATCC GACCACTCGA 
GCAAACTCGG ATCCCATGGT GAGCAAAACG TTGAAATCGC CGAGCCGAAT TGTGCCGCCC 
AAGCGGTACA ATGCCGGTTC GCTGGGCACG GTAGCAGCGA TCGGTGGCCA CCAGCAGCAG 
GCAACGACTC CGCCCACTCC GGCTCCCCGG ACGCCCAACA AGCTACTGCA TACGACTCCA 
AAGAAGGCCG CCACCGCCGG TACGCCCAGG CCAGCAACAC GCACACGGAT ACCAGTAGGT 
TCTGGTCAAA CGCAAGGTAA ATCGCTTCCC TCGTCGAATG CAGCAGTAAA GGTAATGCCG 
AAGAAACCCT TGAACCACGC GGCCCCGGCT GCCAGTAGTA GTCCCAAGGC TACCATCCGG 
AAACCGGTCA GCAGCAGTAG TGGCCGTGTC GCCGAAAAAC CAAACAAAGG AGTCGTCAAA 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of Aedes aegypti, a major arbovirus vector.
Science (New York, N.Y.)316(5832)1718-23(2007 Jun)
Nene V,Wortman JR,Lawson D,Haas B,Kodira C,Tu ZJ,Loftus B,Xi Z,Megy K,Grabherr M,Ren Q,Zdobnov EM,Lobo NF,Campbell KS,Brown SE,Bonaldo MF,Zhu J,Sinkins SP,Hogenkamp DG,Amedeo P,Arensburger P,Atkinson PW,Bidwell S,Biedler J,Birney E,Bruggner RV,Costas J,Coy MR,Crabtree J,Crawford M,Debruyn B,Decaprio D,Eiglmeier K,Eisenstadt E,El-Dorry H,Gelbart WM,Gomes SL,Hammond M,Hannick LI,Hogan JR,Holmes MH,Jaffe D,Johnston JS,Kennedy RC,Koo H,Kravitz S,Kriventseva EV,Kulp D,Labutti K,Lee E,Li S,Lovin DD,Mao C,Mauceli E,Menck CF,Miller JR,Montgomery P,Mori A,Nascimento AL,Naveira HF,Nusbaum C,O'leary S,Orvis J,Pertea M,Quesneville H,Reidenbach KR,Rogers YH,Roth CW,Schneider JR,Schatz M,Shumway M,Stanke M,Stinson EO,Tubio JM,Vanzee JP,Verjovski-Almeida S,Werner D,White O,Wyder S,Zeng Q,Zhao Q,Zhao Y,Hill CA,Raikhel AS,Soares MB,Knudson DL,Lee NH,Galagan J,Salzberg SL,Paulsen IT,Dimopoulos G,Collins FH,Birren B,Fraser-Liggett CM,Severson DW

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LOC5574982 cDNA ORF clone, Aedes aegypti(yellow fever mosquito)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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FAX

オンラインフォーム