GREB1 gene cDNA ORF clone, Acinonyx jubatus(cheetah)

mRNA	Protein	Name
XM_027077913.1	XP_026933714.1	protein GREB1 isoform X2
XM_027077916.1	XP_026933717.1	protein GREB1 isoform X4
XM_027077920.1	XP_026933721.1	protein GREB1 isoform X4
XM_015074747.1	XP_014930233.1	protein GREB1
XM_027077915.1	XP_026933716.1	protein GREB1 isoform X3
XM_027077921.1	XP_026933722.1	protein GREB1 isoform X4
XM_027077917.1	XP_026933718.1	protein GREB1 isoform X4
XM_027077912.1	XP_026933713.1	protein GREB1 isoform X1
XM_027077918.1	XP_026933719.1	protein GREB1 isoform X4
XM_027077919.1	XP_026933720.1	protein GREB1 isoform X4
XM_027077922.1	XP_026933723.1	protein GREB1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAc233926	XM_027077922.1 Latest version!	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAc233922	XM_027077912.1 Latest version!	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAc233923	XM_027077913.1 Latest version!	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAc233924	XM_027077915.1 Latest version!	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAc233925	XM_027077917.1 Latest version!	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAc233925	XM_027077921.1 Latest version!	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAc233925	XM_027077919.1 Latest version!	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAc233925	XM_027077916.1 Latest version!	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAc233925	XM_027077920.1 Latest version!	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAc233925	XM_027077918.1 Latest version!	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAc47770	XM_015074747.1 Latest version!	Acinonyx jubatus growth regulation by estrogen in breast cancer 1 (GREB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

GREB1 ( XM_027077922.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026933723.1 Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OAc233926
Clone ID Related Accession (Same CDS sequence)	XM_027077922.1
Accession Version	XM_027077922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	protein GREB1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834765.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAAG AACACAAGTG CACATCTTGT ACTTCGATGT TCAACAGGGA GGGCTTCCTC 
CCGACCCCGC TCGGAATAGA AAGCGGTGAG CAGCCTCCGT CGTCCTCTCT AAAGATGGGA 
AACTCGTACG CCGGCCAGCT GAAGACGACG CGGTTCGAAG AGGTCTTGCA CAACTCCATC 
GAGGCTTCGC TGCGGTCCAA CAGCCTGGTG CCCAGGCCCG TCTTCTCCCA GTTGTATCTA 
GAAGCCGAAC AGCAGCTCTC CACGCTAGAA GGTGGAGGCC GAGGGGACAA TGAGGATGAG 
GAAGAAGAGG GGGAAGGGGC GCTGGAGCCC AGCGGTCCCC CGAATCCCTA CCAGATGCAC 
CCTCCGCCGG AAGGCTGCTG CACCACAGAC GGGTTCTGCC AGGCGGGGAA GGACCTGCGT 
CTCGTCTCCA TTTCCAGTGA GCCCATAGAC GTCCCTGCAG GCTTTCTCCT CGTGGGGGCC 
AAGTCCCCCA GCCTGCCAGA CCATCTCCTG GTGTGTGCTG TGGACAAAAG GTTCTTGCCG 
GATGACAATG GGCACAACGC TCTTCTTGGT TTCTCTGGGA ATTGCGTTGG CTGTGGAAAG 
AAAGGCTTCT GCTACTTCAC AGAATTCTCT AATCACATAA ATCTCAAGTT GACCACTCAG 
CCCAAGAAGC AGAAACACTT GAAGTATTAC CTGGTCCGCA ACGCACAAGG GACTCTAACC 
AAAGGACCTC TCATCTGTTG GAAAGGCTCA GAGTTCAGAA GCCGGCCGAC CTCCGCCAGT 
GCCTGTTCCA GCTCACTCTT CCCACCAGCG GAGAGCTCCG GGCCTCTGGC TGCCTTCTCC 
AGCGAGCCCG TTCCCGGAGC CAGCCCGAGC GTCCCCCTGG GAGCCCAGCA GGCAGTGCCA 
GCCTCTGAGC ACCCCTCACT TGCTACGGCC GTGGGTCCAG CTGTTTTCAA TGGCAGAGAT 
TCTCCGAAGC ACCAACAGCT GGTGAAGAAC GACCTATCGG CCATGCCACG GCCCTCGGCA 
TTAGGTATCT TATCAAACTC TGGGCCCCCG AAGAAACGCC ACAAAGGGTG GTCCCCTGAA 
TCTCCATCAG CGACAGACGC CGGCTACGCC CAGGGTGGTG GGAACAGAGC TAAGTACGAG 
AGCGCAGGCA TGTCCTGCGT GCCACAGGTT GGCTCGGTGG GACCAGCGTC GGTCACCTTC 
CCCGTTGTGG CCTCTGGAGA ACCAGTGTCC GTCCCTGACA ACTTGCTGAA AATATGCAAG 
GCCAAGCCGG TGATATTTAA AGGCCACGGG AACTTCCCTT ACCTCTGCGG GAACCTGAGT 
GACGTCGTCG TGAGCCCCCT CCTGTACACG TGCTACCAGA ACTCTCAGTC CATCTCCCGG 
GCGTATGAGC ACTACGGGGC CTCGGCGATA CAGCCCATCT CCGAGGAGGT GCAGCTCCTA 
CTGACCGTCT ACTATCTCGT CCAGCTGGCA GCGGACCAGG TGCCCCTGAT GGAGGACCTG 
GAGCAGATCT TCCTGCGCTC GTGGCGCGAA TCGCACCTGA CCGAGATCCG GCAGTACCAG 
CAGGCGCAGC CGCAGCCCTG CCCGCCCGCG CCCAGCGCCG TGGCCCCCGT CACCTCGGCC 
CAGCTGCCCT GGCTCGCCGG CCTGGCCGCC AGCTCCTGCA ACGACAGCGT GCACATCATC 
GAGTGCACCT ACTCCCTGGC CGAGGGCCTC TCCGAGATGT TCCGGCTGCT CATCGAGGGC 
AAGCTCGCCA AGACCAACTA CGTGGTGATC GTCCGCGCGT GCCGCAGCCC CGCCATCGAC 
TCCTGCGTCG CCGTCACGGG AAAATACCAA GCCCGAATTC TTTCTGAGAG CCTCCTCACC 
CCAGCGGAGT ACCAGAAAGA AGTCAATTAC GAGCTGGTTA CCGGGAAAGT GGACTTGTTG 
GGGGCGTTTT TCAGCACCCT CTGTCCAGAG GGGGACATTG ACATTTTGCT GGACAAATTT 
CATCAGGAAA ATCAAGGCCA TATTTCGTCG TCACTTACTG CCTCCTCTGT CACCAAATCA 
GCATCCCTGG ATGCACCGGC ATGCACAAGT TATAATCTGG AGCCCCACCA CATCCGGCCT 
TTCCAGCTGG CCGTGGCTCA GAAGCTCCTC TCCCATGTGT GTTCAATTGC GGATTCCAGC 
ACCCAGAATT TGGACTTAGG ATCCTTCGAG AAGGTGGACT TTCTGATTTG TATTCCACCC 
TCAGAAGTGA CCTATCAGCA AACCCTGTTC CACGTCTGGC ATTCAGGGGT TTTGTCAGAG 
CTCGGCTTGG AAAAGGAGCA CATGACCAAG CAGAGGGTTG AACAGTATGT TTTGAAGCTT 
GATGCTGAAG CGCAGACAAA ATTTAAGTCC TTTCTGCAAA ACTCCTTTCA GAATCCGCAT 
ACGCTCTTTG TCCTGATCCA CGACCACGCC CACTGGGACC TCGTGAGTAG CGCTGTTCAT 
AATCTCTATT CCCAAAGTGA CCCGGCTGTG GGATTGGTGG ACCGGTTGCT GAACTGCAGA 
GAGGTGAAGG AGGCCCCGAA CATCGTGACG CTTCACGTGA CTTCCTTCCC TTACGCTCTG 
CAGACACAGC ACACCCTCAT CAGCCCTTAC AATGAGATCC ACTGGCCCGC CTCCTACAGC 
AATGGGGTGG ACTTATATCC TGAGAACAAG AAGTACTTCG GGCTGTCAGA GTTCATTGAG 
TCCACCCTTT CGGGACACAG CCTCCCTTTG CTCAGATATG ACAGCTCCTT TGAGGCCATG 
GTCACGGCAT TAGGAAAAAG CACTACGCGG CCGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026933723.1
CDS	111..2909
Translation	MRKEHKCTSCTSMFNREGFLPTPLGIESGEQPPSSSLKMGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPVFSQLYLEAEQQLSTLEGGGRGDNEDEEEEGEGALEPSGPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGTLTKGPLICWKGSEFRSRPTSASACSSSLFPPAESSGPLAAFSSEPVPGASPSVPLGAQQAVPASEHPSLATAVGPAVFNGRDSPKHQQLVKNDLSAMPRPSALGILSNSGPPKKRHKGWSPESPSATDAGYAQGGGNRAKYESAGMSCVPQVGSVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLSDVVVSPLLYTCYQNSQSISRAYEHYGASAIQPISEEVQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAQPQPCPPAPSAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIVRACRSPAIDSCVAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDLLGAFFSTLCPEGDIDILLDKFHQENQGHISSSLTASSVTKSASLDAPACTSYNLEPHHIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLSELGLEKEHMTKQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPAVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPASYSNGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKSTTRPP

Target ORF information:

RefSeq Version	XM_027077922.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027077922.1

ORF Insert Sequence:

ATGAGGAAAG AACACAAGTG CACATCTTGT ACTTCGATGT TCAACAGGGA GGGCTTCCTC 
CCGACCCCGC TCGGAATAGA AAGCGGTGAG CAGCCTCCGT CGTCCTCTCT AAAGATGGGA 
AACTCGTACG CCGGCCAGCT GAAGACGACG CGGTTCGAAG AGGTCTTGCA CAACTCCATC 
GAGGCTTCGC TGCGGTCCAA CAGCCTGGTG CCCAGGCCCG TCTTCTCCCA GTTGTATCTA 
GAAGCCGAAC AGCAGCTCTC CACGCTAGAA GGTGGAGGCC GAGGGGACAA TGAGGATGAG 
GAAGAAGAGG GGGAAGGGGC GCTGGAGCCC AGCGGTCCCC CGAATCCCTA CCAGATGCAC 
CCTCCGCCGG AAGGCTGCTG CACCACAGAC GGGTTCTGCC AGGCGGGGAA GGACCTGCGT 
CTCGTCTCCA TTTCCAGTGA GCCCATAGAC GTCCCTGCAG GCTTTCTCCT CGTGGGGGCC 
AAGTCCCCCA GCCTGCCAGA CCATCTCCTG GTGTGTGCTG TGGACAAAAG GTTCTTGCCG 
GATGACAATG GGCACAACGC TCTTCTTGGT TTCTCTGGGA ATTGCGTTGG CTGTGGAAAG 
AAAGGCTTCT GCTACTTCAC AGAATTCTCT AATCACATAA ATCTCAAGTT GACCACTCAG 
CCCAAGAAGC AGAAACACTT GAAGTATTAC CTGGTCCGCA ACGCACAAGG GACTCTAACC 
AAAGGACCTC TCATCTGTTG GAAAGGCTCA GAGTTCAGAA GCCGGCCGAC CTCCGCCAGT 
GCCTGTTCCA GCTCACTCTT CCCACCAGCG GAGAGCTCCG GGCCTCTGGC TGCCTTCTCC 
AGCGAGCCCG TTCCCGGAGC CAGCCCGAGC GTCCCCCTGG GAGCCCAGCA GGCAGTGCCA 
GCCTCTGAGC ACCCCTCACT TGCTACGGCC GTGGGTCCAG CTGTTTTCAA TGGCAGAGAT 
TCTCCGAAGC ACCAACAGCT GGTGAAGAAC GACCTATCGG CCATGCCACG GCCCTCGGCA 
TTAGGTATCT TATCAAACTC TGGGCCCCCG AAGAAACGCC ACAAAGGGTG GTCCCCTGAA 
TCTCCATCAG CGACAGACGC CGGCTACGCC CAGGGTGGTG GGAACAGAGC TAAGTACGAG 
AGCGCAGGCA TGTCCTGCGT GCCACAGGTT GGCTCGGTGG GACCAGCGTC GGTCACCTTC 
CCCGTTGTGG CCTCTGGAGA ACCAGTGTCC GTCCCTGACA ACTTGCTGAA AATATGCAAG 
GCCAAGCCGG TGATATTTAA AGGCCACGGG AACTTCCCTT ACCTCTGCGG GAACCTGAGT 
GACGTCGTCG TGAGCCCCCT CCTGTACACG TGCTACCAGA ACTCTCAGTC CATCTCCCGG 
GCGTATGAGC ACTACGGGGC CTCGGCGATA CAGCCCATCT CCGAGGAGGT GCAGCTCCTA 
CTGACCGTCT ACTATCTCGT CCAGCTGGCA GCGGACCAGG TGCCCCTGAT GGAGGACCTG 
GAGCAGATCT TCCTGCGCTC GTGGCGCGAA TCGCACCTGA CCGAGATCCG GCAGTACCAG 
CAGGCGCAGC CGCAGCCCTG CCCGCCCGCG CCCAGCGCCG TGGCCCCCGT CACCTCGGCC 
CAGCTGCCCT GGCTCGCCGG CCTGGCCGCC AGCTCCTGCA ACGACAGCGT GCACATCATC 
GAGTGCACCT ACTCCCTGGC CGAGGGCCTC TCCGAGATGT TCCGGCTGCT CATCGAGGGC 
AAGCTCGCCA AGACCAACTA CGTGGTGATC GTCCGCGCGT GCCGCAGCCC CGCCATCGAC 
TCCTGCGTCG CCGTCACGGG AAAATACCAA GCCCGAATTC TTTCTGAGAG CCTCCTCACC 
CCAGCGGAGT ACCAGAAAGA AGTCAATTAC GAGCTGGTTA CCGGGAAAGT GGACTTGTTG 
GGGGCGTTTT TCAGCACCCT CTGTCCAGAG GGGGACATTG ACATTTTGCT GGACAAATTT 
CATCAGGAAA ATCAAGGCCA TATTTCGTCG TCACTTACTG CCTCCTCTGT CACCAAATCA 
GCATCCCTGG ATGCACCGGC ATGCACAAGT TATAATCTGG AGCCCCACCA CATCCGGCCT 
TTCCAGCTGG CCGTGGCTCA GAAGCTCCTC TCCCATGTGT GTTCAATTGC GGATTCCAGC 
ACCCAGAATT TGGACTTAGG ATCCTTCGAG AAGGTGGACT TTCTGATTTG TATTCCACCC 
TCAGAAGTGA CCTATCAGCA AACCCTGTTC CACGTCTGGC ATTCAGGGGT TTTGTCAGAG 
CTCGGCTTGG AAAAGGAGCA CATGACCAAG CAGAGGGTTG AACAGTATGT TTTGAAGCTT 
GATGCTGAAG CGCAGACAAA ATTTAAGTCC TTTCTGCAAA ACTCCTTTCA GAATCCGCAT 
ACGCTCTTTG TCCTGATCCA CGACCACGCC CACTGGGACC TCGTGAGTAG CGCTGTTCAT 
AATCTCTATT CCCAAAGTGA CCCGGCTGTG GGATTGGTGG ACCGGTTGCT GAACTGCAGA 
GAGGTGAAGG AGGCCCCGAA CATCGTGACG CTTCACGTGA CTTCCTTCCC TTACGCTCTG 
CAGACACAGC ACACCCTCAT CAGCCCTTAC AATGAGATCC ACTGGCCCGC CTCCTACAGC 
AATGGGGTGG ACTTATATCC TGAGAACAAG AAGTACTTCG GGCTGTCAGA GTTCATTGAG 
TCCACCCTTT CGGGACACAG CCTCCCTTTG CTCAGATATG ACAGCTCCTT TGAGGCCATG 
GTCACGGCAT TAGGAAAAAG CACTACGCGG CCGCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_027077912.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026933713.1 Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc233922
Clone ID Related Accession (Same CDS sequence)	XM_027077912.1
Accession Version	XM_027077912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	protein GREB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834765.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAAG AACACAAGTG CACATCTTGT ACTTCGATGT TCAACAGGGA GGGCTTCCTC 
CCGACCCCGC TCGGAATAGA AAGCGGTGAG CAGCCTCCGT CGTCCTCTCT AAAGATGGGA 
AACTCGTACG CCGGCCAGCT GAAGACGACG CGGTTCGAAG AGGTCTTGCA CAACTCCATC 
GAGGCTTCGC TGCGGTCCAA CAGCCTGGTG CCCAGGCCCG TCTTCTCCCA GTTGTATCTA 
GAAGCCGAAC AGCAGCTCTC CACGCTAGAA GGTGGAGGCC GAGGGGACAA TGAGGATGAG 
GAAGAAGAGG GGGAAGGGGC GCTGGAGCCC AGCGGTCCCC CGAATCCCTA CCAGATGCAC 
CCTCCGCCGG AAGGCTGCTG CACCACAGAC GGGTTCTGCC AGGCGGGGAA GGACCTGCGT 
CTCGTCTCCA TTTCCAGTGA GCCCATAGAC GTCCCTGCAG GCTTTCTCCT CGTGGGGGCC 
AAGTCCCCCA GCCTGCCAGA CCATCTCCTG GTGTGTGCTG TGGACAAAAG GTTCTTGCCG 
GATGACAATG GGCACAACGC TCTTCTTGGT TTCTCTGGGA ATTGCGTTGG CTGTGGAAAG 
AAAGGCTTCT GCTACTTCAC AGAATTCTCT AATCACATAA ATCTCAAGTT GACCACTCAG 
CCCAAGAAGC AGAAACACTT GAAGTATTAC CTGGTCCGCA ACGCACAAGG GACTCTAACC 
AAAGGACCTC TCATCTGTTG GAAAGGCTCA GAGTTCAGAA GCCGGCCGAC CTCCGCCAGT 
GCCTGTTCCA GCTCACTCTT CCCACCAGCG GAGAGCTCCG GGCCTCTGGC TGCCTTCTCC 
AGCGAGCCCG TTCCCGGAGC CAGCCCGAGC GTCCCCCTGG GAGCCCAGCA GGCAGTGCCA 
GCCTCTGAGC ACCCCTCACT TGCTACGGCC GTGGGTCCAG CTGTTTTCAA TGGCAGAGAT 
TCTCCGAAGC ACCAACAGCT GGTGAAGAAC GACCTATCGG CCATGCCACG GCCCTCGGCA 
TTAGGTATCT TATCAAACTC TGGGCCCCCG AAGAAACGCC ACAAAGGGTG GTCCCCTGAA 
TCTCCATCAG CGACAGACGC CGGCTACGCC CAGGGTGGTG GGAACAGAGC TAAGTACGAG 
AGCGCAGGCA TGTCCTGCGT GCCACAGGTT GGCTCGGTGG GACCAGCGTC GGTCACCTTC 
CCCGTTGTGG CCTCTGGAGA ACCAGTGTCC GTCCCTGACA ACTTGCTGAA AATATGCAAG 
GCCAAGCCGG TGATATTTAA AGGCCACGGG AACTTCCCTT ACCTCTGCGG GAACCTGAGT 
GACGTCGTCG TGAGCCCCCT CCTGTACACG TGCTACCAGA ACTCTCAGTC CATCTCCCGG 
GCGTATGAGC ACTACGGGGC CTCGGCGATA CAGCCCATCT CCGAGGAGGT GCAGCTCCTA 
CTGACCGTCT ACTATCTCGT CCAGCTGGCA GCGGACCAGG TGCCCCTGAT GGAGGACCTG 
GAGCAGATCT TCCTGCGCTC GTGGCGCGAA TCGCACCTGA CCGAGATCCG GCAGTACCAG 
CAGGCGCAGC CGCAGCCCTG CCCGCCCGCG CCCAGCGCCG TGGCCCCCGT CACCTCGGCC 
CAGCTGCCCT GGCTCGCCGG CCTGGCCGCC AGCTCCTGCA ACGACAGCGT GCACATCATC 
GAGTGCACCT ACTCCCTGGC CGAGGGCCTC TCCGAGATGT TCCGGCTGCT CATCGAGGGC 
AAGCTCGCCA AGACCAACTA CGTGGTGATC GTCCGCGCGT GCCGCAGCCC CGCCATCGAC 
TCCTGCGTCG CCGTCACGGG AAAATACCAA GCCCGAATTC TTTCTGAGAG CCTCCTCACC 
CCAGCGGAGT ACCAGAAAGA AGTCAATTAC GAGCTGGTTA CCGGGAAAGT GGACTTGTTG 
GGGGCGTTTT TCAGCACCCT CTGTCCAGAG GGGGACATTG ACATTTTGCT GGACAAATTT 
CATCAGGAAA ATCAAGGCCA TATTTCGTCG TCACTTACTG CCTCCTCTGT CACCAAATCA 
GCATCCCTGG ATGCACCGGC ATGCACAAGT TATAATCTGG AGCCCCACCA CATCCGGCCT 
TTCCAGCTGG CCGTGGCTCA GAAGCTCCTC TCCCATGTGT GTTCAATTGC GGATTCCAGC 
ACCCAGAATT TGGACTTAGG ATCCTTCGAG AAGGTGGACT TTCTGATTTG TATTCCACCC 
TCAGAAGTGA CCTATCAGCA AACCCTGTTC CACGTCTGGC ATTCAGGGGT TTTGTCAGAG 
CTCGGCTTGG AAAAGGAGCA CATGACCAAG CAGAGGGTTG AACAGTATGT TTTGAAGCTT 
GATGCTGAAG CGCAGACAAA ATTTAAGTCC TTTCTGCAAA ACTCCTTTCA GAATCCGCAT 
ACGCTCTTTG TCCTGATCCA CGACCACGCC CACTGGGACC TCGTGAGTAG CGCTGTTCAT 
AATCTCTATT CCCAAAGTGA CCCGGCTGTG GGATTGGTGG ACCGGTTGCT GAACTGCAGA 
GAGGTGAAGG AGGCCCCGAA CATCGTGACG CTTCACGTGA CTTCCTTCCC TTACGCTCTG 
CAGACACAGC ACACCCTCAT CAGCCCTTAC AATGAGATCC ACTGGCCCGC CTCCTACAGC 
AATGGGGTGG ACTTATATCC TGAGAACAAG AAGTACTTCG GGCTGTCAGA GTTCATTGAG 
TCCACCCTTT CGGGACACAG CCTCCCTTTG CTCAGATATG ACAGCTCCTT TGAGGCCATG 
GTCACGGCAT TAGGAAAAAG GTTCCCCCGC CTGCACAGCG CGGTGATCAG GACCTTTGTT 
CTCGTGCAGC ACTACGCGGC CGCCATGATG GCCGTGAGCG GCCTGTCGCA GATGAAGAAC 
TACACGTCGG TGGAGACGCT GGAGATCACC CAGAACCTCA TCAACTCCCC CAAGCAGTGC 
CCCTGCGGCC ACGGGCTCAT GGTCCTGCTG CGGGTGCCCT GCTCCCCGCT GGCGGCGGTG 
GCCTACGAGC GGCTGGCCCA CGTGCGGGCA CGCCTGGCCC TGGAGGAGCA CTTTGAGATC 
ATCCTGGGCA ACCCCAGCTC CGGCATCACC GTCGGGAAGC ACTTCGTGAA GCAACTCAAG 
CTGTGGCAGA AGATCGAGGA CGCCGAGTGG AGACCTCAGA CCTACCTGGA GCTGGAGGGC 
CTGCCCTGCA TCCTGATCTT CAGTGGGATG GACCCTCACG GGGAATCCTT ACCAAGGTCT 
CTGAGGTACT GTGACCTGCG TCTGATAAAC TCCTCCTGCT TGGTGAGAAC AGCGCTGGAG 
CAGGAGCTGG GCCTGGCCGC TTACTTTGTG AGCAACGAGG TTTCTTTGGA GAAGGCGGCC 
CGGAACGAGC CCTTGGAGAG CGATGCGGAA AAGCTGAGCA GCACAGACAA CGAGGACGAG 
GAGCTGGTGA CGGAAGGCTC CACCTCGGAG AAGAGGAGCC CGGTGAAGCG GGAGAGGCCC 
TGCTCCCATG ATTCGGCGGC GTCCTCCCTC TCCTCGAAAG CTTCCGGCTC GGCACCGTGC 
CGCGAGTCCT CGGCTCAGCC CGGGGCGCCC TCGCAGGGGG AGCGGGCCCG ATCACCGCAG 
CCCTGCGGCC CGTCGGAGGA GGGCAGGGCC CCCGGGGAGA GGCAGAGGCC CCACGCTGGC 
CGGGGACCGC CGTCGGTCAT CAGCAGGCGC AGCCCCGGGC TGGCCGCCCA GCCGGACTCC 
GGCCCCAGGA CCAGCCAGAG GAGCGTCCAG GTGTCGGTCA CCCCGTCGTC CTCCCAGCTG 
TCCTCCTCCG GCTCGTCGTC CTCGTCCGGG GCGCTCGTCC CGCAGGCCTC CCGGTGCTCC 
CTGACCAAGG CCTGCCGCCA GCCGCCCGTC GTCTTCCTGC CCAAGCTGGT GTATGACATG 
GTCACGGCCA CCGACAGCAG CGGCCTGCCC AAGGCCGCCT CGCTCCTGCC CTCCCACTCG 
GTCATGTGGG CCAGCTCCTT CCGCCCGCTG CTCAGCAAGA CCATGACGTC CACGGAGCAG 
TCCCTCTACT ACCGGCAGTG GACGGTGCCC CGGCCCAGCC ACATGGACTA CAGCAACCGC 
ACCGAGGGCC GCGTGGACGG CTTCCACCCG CGCCGGCTGC TGCTCAGCGG GCCCCCTCAG 
GTTGGGAAGA CAGGTGCTTA CCTGCAGTTC CTTAGTATTT TGTCCAGAAT GCTCATCCGG 
CTGACGGAAG TGGATGTTTA TGATGAAGAA GAGATCAATA TCCACCTCAG AGAGGAGTCC 
GATTGGCATT ACCTCCAGCT CAGTGACCCC TGGCCAGACC TGGAGCTATT CAAGAAGTTG 
CCTTTTGACT ACATTATTCA CGACCCGAAG TATGAAGACG CCAGTCTGAT CTGTTCGCAC 
CTTCAGAGCG TGAAGAGTGA AGCCAGGGGG ACGTCCCGGA AGCCGGAGGA GCTCTACGTG 
CGGCGCCAGA CAGCGCGGAT GAGGCTGTCC AAGTACGCGG CCTATAACAC ATACCACCAC 
TGCGAGCAGT GTCACCACTA CATGGGCTTC CACCCCCGCT ACCAGCTCTA TGAGTCCACC 
CTGCACGCCT TTGCCTTCTC TTACTCCATG CTAGGAGAGG AAATCCAGCT ACATTTCATC 
ATCCCCAAGT CCAAAGAGCA CCACTTTGTC TTCAGCCAAC CCGGAGGCCA GCTGGAGAGC 
ATGCGGCTGC CCCTGGTCAC AGACAAGAGC CACGAGTATA TAAAAAGTCC GACGTTCACC 
CCGACAACCG GCCGTCACGA ACACGGACTC TTTAACCTGT ACCATGCCAT GGATGGTGCC 
AGCCATCTGC ACGTGCTGGT CGTCAAGGAG TATGAAATGG CCATTTATAA GAAATACTGG 
CCCAACCACA TCATGCTGGT GCTCCCAAGT ATCTTCAACA GCGCTGGAGT TGGCGCCGCC 
CACTTCCTGA TCAAGGAGCT GTCTTACCAC AATCTGGAGC TGGAGAGGAA CCGGCAGGAG 
GAGCTGGGGA TCAAGCCACA GGACATCTGG CCTTTCATCG TGATCTCGGA CGACTCCTGC 
GTGATGTGGA ACGTGGTGGA TGTAGACTGT GGCGGAGAAA GAAGCAGGGA GTTTTCCTGG 
GCAGAAAGAA ACGTGTCTTT GAAGCACATC ATGCAGCACA TCGAGGCGTC CCCCAACATC 
ACCCACTATG CCCTGATTGG CATGCGGAAG TGGTCCAGCA AGACGGGGAG CGCCGAGGTG 
CGGGAGCCCT TCTCCCGCTG CCACGTGCAC GACTTCATCA TTCTGAACGT GGACCTGACC 
CAGAACGTGC AGTACCACCA GAACCGGTTC ACGTGTGACG ACGTGGACTT CAACCTGCGG 
GTGCATAGCG CCGGCCTCCT GCTCTGCCGT TTCAACCGCT TTAGCGTGAT GAAGAAGCAG 
ATTGCCGTGG GTGGGCACAG GTCCTGCCAC ATCACGTCCA AGGTATCCGA CAACCCCGTG 
GCCATCGTGC CGGCCCAGTA CATCTGCGCC CCGGACAGCA AGCACACGTT CCTCGCGGCG 
CCCGCGCAGC TCCTGCTGGA GAAGTTCCTC CAGCACCACA GTCACCGATT CTTCCCGCTG 
TCCCTGAGGA ACCGCGGCCA CCCTGTGCTC TCGGTCGACT GCTACCTTAA CCTGGGATCT 
CAGATTTCTG TGTGTTATGT GAGCTCCAGG CCCCACTCCC TGAACATCAG CTGCTCTGAC 
TTCGTGTTTA GCGGACTCCT GCTATACCTC TGTGATTCTT TTGTGGGAGC TAGCTTTTTG 
AAAAAGTTTC ATTTTCTGAA AGGTGCCACC CTGTGCGTGA TCTGCCAGGA CCGGAACTCC 
CTGCGCCAGA CGGTGGTCCG CCTCGAGCTG GAAGACGAGT GGCAGTTCCG GCTGCGTGAC 
GAATTCCAGA CGGCCAACGC CAAGGAAGAC CGGCCGCTCT TTTTTCTGAC CGGGCGGCAC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026933713.1
CDS	84..6029
Translation	MRKEHKCTSCTSMFNREGFLPTPLGIESGEQPPSSSLKMGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPVFSQLYLEAEQQLSTLEGGGRGDNEDEEEEGEGALEPSGPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGTLTKGPLICWKGSEFRSRPTSASACSSSLFPPAESSGPLAAFSSEPVPGASPSVPLGAQQAVPASEHPSLATAVGPAVFNGRDSPKHQQLVKNDLSAMPRPSALGILSNSGPPKKRHKGWSPESPSATDAGYAQGGGNRAKYESAGMSCVPQVGSVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLSDVVVSPLLYTCYQNSQSISRAYEHYGASAIQPISEEVQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAQPQPCPPAPSAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIVRACRSPAIDSCVAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDLLGAFFSTLCPEGDIDILLDKFHQENQGHISSSLTASSVTKSASLDAPACTSYNLEPHHIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLSELGLEKEHMTKQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPAVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPASYSNGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPSSGITVGKHFVKQLKLWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNEVSLEKAARNEPLESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERPCSHDSAASSLSSKASGSAPCRESSAQPGAPSQGERARSPQPCGPSEEGRAPGERQRPHAGRGPPSVISRRSPGLAAQPDSGPRTSQRSVQVSVTPSSSQLSSSGSSSSSGALVPQASRCSLTKACRQPPVVFLPKLVYDMVTATDSSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPSHMDYSNRTEGRVDGFHPRRLLLSGPPQVGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINIHLREESDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHLQSVKSEARGTSRKPEELYVRRQTARMRLSKYAAYNTYHHCEQCHHYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDCGGERSREFSWAERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSAEVREPFSRCHVHDFIILNVDLTQNVQYHQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSCHITSKVSDNPVAIVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLRNRGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_027077912.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027077912.1

ORF Insert Sequence:

ATGAGGAAAG AACACAAGTG CACATCTTGT ACTTCGATGT TCAACAGGGA GGGCTTCCTC 
CCGACCCCGC TCGGAATAGA AAGCGGTGAG CAGCCTCCGT CGTCCTCTCT AAAGATGGGA 
AACTCGTACG CCGGCCAGCT GAAGACGACG CGGTTCGAAG AGGTCTTGCA CAACTCCATC 
GAGGCTTCGC TGCGGTCCAA CAGCCTGGTG CCCAGGCCCG TCTTCTCCCA GTTGTATCTA 
GAAGCCGAAC AGCAGCTCTC CACGCTAGAA GGTGGAGGCC GAGGGGACAA TGAGGATGAG 
GAAGAAGAGG GGGAAGGGGC GCTGGAGCCC AGCGGTCCCC CGAATCCCTA CCAGATGCAC 
CCTCCGCCGG AAGGCTGCTG CACCACAGAC GGGTTCTGCC AGGCGGGGAA GGACCTGCGT 
CTCGTCTCCA TTTCCAGTGA GCCCATAGAC GTCCCTGCAG GCTTTCTCCT CGTGGGGGCC 
AAGTCCCCCA GCCTGCCAGA CCATCTCCTG GTGTGTGCTG TGGACAAAAG GTTCTTGCCG 
GATGACAATG GGCACAACGC TCTTCTTGGT TTCTCTGGGA ATTGCGTTGG CTGTGGAAAG 
AAAGGCTTCT GCTACTTCAC AGAATTCTCT AATCACATAA ATCTCAAGTT GACCACTCAG 
CCCAAGAAGC AGAAACACTT GAAGTATTAC CTGGTCCGCA ACGCACAAGG GACTCTAACC 
AAAGGACCTC TCATCTGTTG GAAAGGCTCA GAGTTCAGAA GCCGGCCGAC CTCCGCCAGT 
GCCTGTTCCA GCTCACTCTT CCCACCAGCG GAGAGCTCCG GGCCTCTGGC TGCCTTCTCC 
AGCGAGCCCG TTCCCGGAGC CAGCCCGAGC GTCCCCCTGG GAGCCCAGCA GGCAGTGCCA 
GCCTCTGAGC ACCCCTCACT TGCTACGGCC GTGGGTCCAG CTGTTTTCAA TGGCAGAGAT 
TCTCCGAAGC ACCAACAGCT GGTGAAGAAC GACCTATCGG CCATGCCACG GCCCTCGGCA 
TTAGGTATCT TATCAAACTC TGGGCCCCCG AAGAAACGCC ACAAAGGGTG GTCCCCTGAA 
TCTCCATCAG CGACAGACGC CGGCTACGCC CAGGGTGGTG GGAACAGAGC TAAGTACGAG 
AGCGCAGGCA TGTCCTGCGT GCCACAGGTT GGCTCGGTGG GACCAGCGTC GGTCACCTTC 
CCCGTTGTGG CCTCTGGAGA ACCAGTGTCC GTCCCTGACA ACTTGCTGAA AATATGCAAG 
GCCAAGCCGG TGATATTTAA AGGCCACGGG AACTTCCCTT ACCTCTGCGG GAACCTGAGT 
GACGTCGTCG TGAGCCCCCT CCTGTACACG TGCTACCAGA ACTCTCAGTC CATCTCCCGG 
GCGTATGAGC ACTACGGGGC CTCGGCGATA CAGCCCATCT CCGAGGAGGT GCAGCTCCTA 
CTGACCGTCT ACTATCTCGT CCAGCTGGCA GCGGACCAGG TGCCCCTGAT GGAGGACCTG 
GAGCAGATCT TCCTGCGCTC GTGGCGCGAA TCGCACCTGA CCGAGATCCG GCAGTACCAG 
CAGGCGCAGC CGCAGCCCTG CCCGCCCGCG CCCAGCGCCG TGGCCCCCGT CACCTCGGCC 
CAGCTGCCCT GGCTCGCCGG CCTGGCCGCC AGCTCCTGCA ACGACAGCGT GCACATCATC 
GAGTGCACCT ACTCCCTGGC CGAGGGCCTC TCCGAGATGT TCCGGCTGCT CATCGAGGGC 
AAGCTCGCCA AGACCAACTA CGTGGTGATC GTCCGCGCGT GCCGCAGCCC CGCCATCGAC 
TCCTGCGTCG CCGTCACGGG AAAATACCAA GCCCGAATTC TTTCTGAGAG CCTCCTCACC 
CCAGCGGAGT ACCAGAAAGA AGTCAATTAC GAGCTGGTTA CCGGGAAAGT GGACTTGTTG 
GGGGCGTTTT TCAGCACCCT CTGTCCAGAG GGGGACATTG ACATTTTGCT GGACAAATTT 
CATCAGGAAA ATCAAGGCCA TATTTCGTCG TCACTTACTG CCTCCTCTGT CACCAAATCA 
GCATCCCTGG ATGCACCGGC ATGCACAAGT TATAATCTGG AGCCCCACCA CATCCGGCCT 
TTCCAGCTGG CCGTGGCTCA GAAGCTCCTC TCCCATGTGT GTTCAATTGC GGATTCCAGC 
ACCCAGAATT TGGACTTAGG ATCCTTCGAG AAGGTGGACT TTCTGATTTG TATTCCACCC 
TCAGAAGTGA CCTATCAGCA AACCCTGTTC CACGTCTGGC ATTCAGGGGT TTTGTCAGAG 
CTCGGCTTGG AAAAGGAGCA CATGACCAAG CAGAGGGTTG AACAGTATGT TTTGAAGCTT 
GATGCTGAAG CGCAGACAAA ATTTAAGTCC TTTCTGCAAA ACTCCTTTCA GAATCCGCAT 
ACGCTCTTTG TCCTGATCCA CGACCACGCC CACTGGGACC TCGTGAGTAG CGCTGTTCAT 
AATCTCTATT CCCAAAGTGA CCCGGCTGTG GGATTGGTGG ACCGGTTGCT GAACTGCAGA 
GAGGTGAAGG AGGCCCCGAA CATCGTGACG CTTCACGTGA CTTCCTTCCC TTACGCTCTG 
CAGACACAGC ACACCCTCAT CAGCCCTTAC AATGAGATCC ACTGGCCCGC CTCCTACAGC 
AATGGGGTGG ACTTATATCC TGAGAACAAG AAGTACTTCG GGCTGTCAGA GTTCATTGAG 
TCCACCCTTT CGGGACACAG CCTCCCTTTG CTCAGATATG ACAGCTCCTT TGAGGCCATG 
GTCACGGCAT TAGGAAAAAG GTTCCCCCGC CTGCACAGCG CGGTGATCAG GACCTTTGTT 
CTCGTGCAGC ACTACGCGGC CGCCATGATG GCCGTGAGCG GCCTGTCGCA GATGAAGAAC 
TACACGTCGG TGGAGACGCT GGAGATCACC CAGAACCTCA TCAACTCCCC CAAGCAGTGC 
CCCTGCGGCC ACGGGCTCAT GGTCCTGCTG CGGGTGCCCT GCTCCCCGCT GGCGGCGGTG 
GCCTACGAGC GGCTGGCCCA CGTGCGGGCA CGCCTGGCCC TGGAGGAGCA CTTTGAGATC 
ATCCTGGGCA ACCCCAGCTC CGGCATCACC GTCGGGAAGC ACTTCGTGAA GCAACTCAAG 
CTGTGGCAGA AGATCGAGGA CGCCGAGTGG AGACCTCAGA CCTACCTGGA GCTGGAGGGC 
CTGCCCTGCA TCCTGATCTT CAGTGGGATG GACCCTCACG GGGAATCCTT ACCAAGGTCT 
CTGAGGTACT GTGACCTGCG TCTGATAAAC TCCTCCTGCT TGGTGAGAAC AGCGCTGGAG 
CAGGAGCTGG GCCTGGCCGC TTACTTTGTG AGCAACGAGG TTTCTTTGGA GAAGGCGGCC 
CGGAACGAGC CCTTGGAGAG CGATGCGGAA AAGCTGAGCA GCACAGACAA CGAGGACGAG 
GAGCTGGTGA CGGAAGGCTC CACCTCGGAG AAGAGGAGCC CGGTGAAGCG GGAGAGGCCC 
TGCTCCCATG ATTCGGCGGC GTCCTCCCTC TCCTCGAAAG CTTCCGGCTC GGCACCGTGC 
CGCGAGTCCT CGGCTCAGCC CGGGGCGCCC TCGCAGGGGG AGCGGGCCCG ATCACCGCAG 
CCCTGCGGCC CGTCGGAGGA GGGCAGGGCC CCCGGGGAGA GGCAGAGGCC CCACGCTGGC 
CGGGGACCGC CGTCGGTCAT CAGCAGGCGC AGCCCCGGGC TGGCCGCCCA GCCGGACTCC 
GGCCCCAGGA CCAGCCAGAG GAGCGTCCAG GTGTCGGTCA CCCCGTCGTC CTCCCAGCTG 
TCCTCCTCCG GCTCGTCGTC CTCGTCCGGG GCGCTCGTCC CGCAGGCCTC CCGGTGCTCC 
CTGACCAAGG CCTGCCGCCA GCCGCCCGTC GTCTTCCTGC CCAAGCTGGT GTATGACATG 
GTCACGGCCA CCGACAGCAG CGGCCTGCCC AAGGCCGCCT CGCTCCTGCC CTCCCACTCG 
GTCATGTGGG CCAGCTCCTT CCGCCCGCTG CTCAGCAAGA CCATGACGTC CACGGAGCAG 
TCCCTCTACT ACCGGCAGTG GACGGTGCCC CGGCCCAGCC ACATGGACTA CAGCAACCGC 
ACCGAGGGCC GCGTGGACGG CTTCCACCCG CGCCGGCTGC TGCTCAGCGG GCCCCCTCAG 
GTTGGGAAGA CAGGTGCTTA CCTGCAGTTC CTTAGTATTT TGTCCAGAAT GCTCATCCGG 
CTGACGGAAG TGGATGTTTA TGATGAAGAA GAGATCAATA TCCACCTCAG AGAGGAGTCC 
GATTGGCATT ACCTCCAGCT CAGTGACCCC TGGCCAGACC TGGAGCTATT CAAGAAGTTG 
CCTTTTGACT ACATTATTCA CGACCCGAAG TATGAAGACG CCAGTCTGAT CTGTTCGCAC 
CTTCAGAGCG TGAAGAGTGA AGCCAGGGGG ACGTCCCGGA AGCCGGAGGA GCTCTACGTG 
CGGCGCCAGA CAGCGCGGAT GAGGCTGTCC AAGTACGCGG CCTATAACAC ATACCACCAC 
TGCGAGCAGT GTCACCACTA CATGGGCTTC CACCCCCGCT ACCAGCTCTA TGAGTCCACC 
CTGCACGCCT TTGCCTTCTC TTACTCCATG CTAGGAGAGG AAATCCAGCT ACATTTCATC 
ATCCCCAAGT CCAAAGAGCA CCACTTTGTC TTCAGCCAAC CCGGAGGCCA GCTGGAGAGC 
ATGCGGCTGC CCCTGGTCAC AGACAAGAGC CACGAGTATA TAAAAAGTCC GACGTTCACC 
CCGACAACCG GCCGTCACGA ACACGGACTC TTTAACCTGT ACCATGCCAT GGATGGTGCC 
AGCCATCTGC ACGTGCTGGT CGTCAAGGAG TATGAAATGG CCATTTATAA GAAATACTGG 
CCCAACCACA TCATGCTGGT GCTCCCAAGT ATCTTCAACA GCGCTGGAGT TGGCGCCGCC 
CACTTCCTGA TCAAGGAGCT GTCTTACCAC AATCTGGAGC TGGAGAGGAA CCGGCAGGAG 
GAGCTGGGGA TCAAGCCACA GGACATCTGG CCTTTCATCG TGATCTCGGA CGACTCCTGC 
GTGATGTGGA ACGTGGTGGA TGTAGACTGT GGCGGAGAAA GAAGCAGGGA GTTTTCCTGG 
GCAGAAAGAA ACGTGTCTTT GAAGCACATC ATGCAGCACA TCGAGGCGTC CCCCAACATC 
ACCCACTATG CCCTGATTGG CATGCGGAAG TGGTCCAGCA AGACGGGGAG CGCCGAGGTG 
CGGGAGCCCT TCTCCCGCTG CCACGTGCAC GACTTCATCA TTCTGAACGT GGACCTGACC 
CAGAACGTGC AGTACCACCA GAACCGGTTC ACGTGTGACG ACGTGGACTT CAACCTGCGG 
GTGCATAGCG CCGGCCTCCT GCTCTGCCGT TTCAACCGCT TTAGCGTGAT GAAGAAGCAG 
ATTGCCGTGG GTGGGCACAG GTCCTGCCAC ATCACGTCCA AGGTATCCGA CAACCCCGTG 
GCCATCGTGC CGGCCCAGTA CATCTGCGCC CCGGACAGCA AGCACACGTT CCTCGCGGCG 
CCCGCGCAGC TCCTGCTGGA GAAGTTCCTC CAGCACCACA GTCACCGATT CTTCCCGCTG 
TCCCTGAGGA ACCGCGGCCA CCCTGTGCTC TCGGTCGACT GCTACCTTAA CCTGGGATCT 
CAGATTTCTG TGTGTTATGT GAGCTCCAGG CCCCACTCCC TGAACATCAG CTGCTCTGAC 
TTCGTGTTTA GCGGACTCCT GCTATACCTC TGTGATTCTT TTGTGGGAGC TAGCTTTTTG 
AAAAAGTTTC ATTTTCTGAA AGGTGCCACC CTGTGCGTGA TCTGCCAGGA CCGGAACTCC 
CTGCGCCAGA CGGTGGTCCG CCTCGAGCTG GAAGACGAGT GGCAGTTCCG GCTGCGTGAC 
GAATTCCAGA CGGCCAACGC CAAGGAAGAC CGGCCGCTCT TTTTTCTGAC CGGGCGGCAC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_027077913.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026933714.1 Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X2, mRNA.

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CloneID	OAc233923
Clone ID Related Accession (Same CDS sequence)	XM_027077913.1
Accession Version	XM_027077913.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	protein GREB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834765.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAAG AACACAAGTG CACATCTTGT ACTTCGATGT TCAACAGGGA GGGCTTCCTC 
CCGACCCCGC TCGGAATAGA AAGCGGTGAG CAGCCTCCGT CGTCCTCTCT AAAGATGGGA 
AACTCGTACG CCGGCCAGCT GAAGACGACG CGGTTCGAAG AGGTCTTGCA CAACTCCATC 
GAGGCTTCGC TGCGGTCCAA CAGCCTGGTG CCCAGGCCCG TCTTCTCCCA GTTGTATCTA 
GAAGCCGAAC AGCAGCTCTC CACGCTAGAA GGTGGAGGCC GAGGGGACAA TGAGGATGAG 
GAAGAAGAGG GGGAAGGGGC GCTGGAGCCC AGCGGTCCCC CGAATCCCTA CCAGATGCAC 
CCTCCGCCGG AAGGCTGCTG CACCACAGAC GGGTTCTGCC AGGCGGGGAA GGACCTGCGT 
CTCGTCTCCA TTTCCAGTGA GCCCATAGAC GTCCCTGCAG GCTTTCTCCT CGTGGGGGCC 
AAGTCCCCCA GCCTGCCAGA CCATCTCCTG GTGTGTGCTG TGGACAAAAG GTTCTTGCCG 
GATGACAATG GGCACAACGC TCTTCTTGGT TTCTCTGGGA ATTGCGTTGG CTGTGGAAAG 
AAAGGCTTCT GCTACTTCAC AGAATTCTCT AATCACATAA ATCTCAAGTT GACCACTCAG 
CCCAAGAAGC AGAAACACTT GAAGTATTAC CTGGTCCGCA ACGCACAAGG GACTCTAACC 
AAAGGACCTC TCATCTGTTG GAAAGGCTCA GAGTTCAGAA GCCGGCCGAC CTCCGCCAGT 
GCCTGTTCCA GCTCACTCTT CCCACCAGCG GAGAGCTCCG GGCCTCTGGC TGCCTTCTCC 
AGCGAGCCCG TTCCCGGAGC CAGCCCGAGC GTCCCCCTGG GAGCCCAGCA GGCAGTGCCA 
GCCTCTGAGC ACCCCTCACT TGCTACGGCC GTGGGTCCAG CTGTTTTCAA TGGCAGAGAT 
TCTCCGAAGC ACCAACAGCT GGTGAAGAAC GACCTATCGG CCATGCCACG GCCCTCGGCA 
TTAGGTATCT TATCAAACTC TGGGCCCCCG AAGAAACGCC ACAAAGGGTG GTCCCCTGAA 
TCTCCATCAG CGACAGACGC CGGCTACGCC CAGGGTGGTG GGAACAGAGC TAAGTACGAG 
AGCGCAGGCA TGTCCTGCGT GCCACAGGTT GGCTCGGTGG GACCAGCGTC GGTCACCTTC 
CCCGTTGTGG CCTCTGGAGA ACCAGTGTCC GTCCCTGACA ACTTGCTGAA AATATGCAAG 
GCCAAGCCGG TGATATTTAA AGGCCACGGG AACTTCCCTT ACCTCTGCGG GAACCTGAGT 
GACGTCGTCG TGAGCCCCCT CCTGTACACG TGCTACCAGA ACTCTCAGTC CATCTCCCGG 
GCGTATGAGC ACTACGGGGC CTCGGCGATA CAGCCCATCT CCGAGGAGGT GCAGCTCCTA 
CTGACCGTCT ACTATCTCGT CCAGCTGGCG GACCAGGTGC CCCTGATGGA GGACCTGGAG 
CAGATCTTCC TGCGCTCGTG GCGCGAATCG CACCTGACCG AGATCCGGCA GTACCAGCAG 
GCGCAGCCGC AGCCCTGCCC GCCCGCGCCC AGCGCCGTGG CCCCCGTCAC CTCGGCCCAG 
CTGCCCTGGC TCGCCGGCCT GGCCGCCAGC TCCTGCAACG ACAGCGTGCA CATCATCGAG 
TGCACCTACT CCCTGGCCGA GGGCCTCTCC GAGATGTTCC GGCTGCTCAT CGAGGGCAAG 
CTCGCCAAGA CCAACTACGT GGTGATCGTC CGCGCGTGCC GCAGCCCCGC CATCGACTCC 
TGCGTCGCCG TCACGGGAAA ATACCAAGCC CGAATTCTTT CTGAGAGCCT CCTCACCCCA 
GCGGAGTACC AGAAAGAAGT CAATTACGAG CTGGTTACCG GGAAAGTGGA CTTGTTGGGG 
GCGTTTTTCA GCACCCTCTG TCCAGAGGGG GACATTGACA TTTTGCTGGA CAAATTTCAT 
CAGGAAAATC AAGGCCATAT TTCGTCGTCA CTTACTGCCT CCTCTGTCAC CAAATCAGCA 
TCCCTGGATG CACCGGCATG CACAAGTTAT AATCTGGAGC CCCACCACAT CCGGCCTTTC 
CAGCTGGCCG TGGCTCAGAA GCTCCTCTCC CATGTGTGTT CAATTGCGGA TTCCAGCACC 
CAGAATTTGG ACTTAGGATC CTTCGAGAAG GTGGACTTTC TGATTTGTAT TCCACCCTCA 
GAAGTGACCT ATCAGCAAAC CCTGTTCCAC GTCTGGCATT CAGGGGTTTT GTCAGAGCTC 
GGCTTGGAAA AGGAGCACAT GACCAAGCAG AGGGTTGAAC AGTATGTTTT GAAGCTTGAT 
GCTGAAGCGC AGACAAAATT TAAGTCCTTT CTGCAAAACT CCTTTCAGAA TCCGCATACG 
CTCTTTGTCC TGATCCACGA CCACGCCCAC TGGGACCTCG TGAGTAGCGC TGTTCATAAT 
CTCTATTCCC AAAGTGACCC GGCTGTGGGA TTGGTGGACC GGTTGCTGAA CTGCAGAGAG 
GTGAAGGAGG CCCCGAACAT CGTGACGCTT CACGTGACTT CCTTCCCTTA CGCTCTGCAG 
ACACAGCACA CCCTCATCAG CCCTTACAAT GAGATCCACT GGCCCGCCTC CTACAGCAAT 
GGGGTGGACT TATATCCTGA GAACAAGAAG TACTTCGGGC TGTCAGAGTT CATTGAGTCC 
ACCCTTTCGG GACACAGCCT CCCTTTGCTC AGATATGACA GCTCCTTTGA GGCCATGGTC 
ACGGCATTAG GAAAAAGGTT CCCCCGCCTG CACAGCGCGG TGATCAGGAC CTTTGTTCTC 
GTGCAGCACT ACGCGGCCGC CATGATGGCC GTGAGCGGCC TGTCGCAGAT GAAGAACTAC 
ACGTCGGTGG AGACGCTGGA GATCACCCAG AACCTCATCA ACTCCCCCAA GCAGTGCCCC 
TGCGGCCACG GGCTCATGGT CCTGCTGCGG GTGCCCTGCT CCCCGCTGGC GGCGGTGGCC 
TACGAGCGGC TGGCCCACGT GCGGGCACGC CTGGCCCTGG AGGAGCACTT TGAGATCATC 
CTGGGCAACC CCAGCTCCGG CATCACCGTC GGGAAGCACT TCGTGAAGCA ACTCAAGCTG 
TGGCAGAAGA TCGAGGACGC CGAGTGGAGA CCTCAGACCT ACCTGGAGCT GGAGGGCCTG 
CCCTGCATCC TGATCTTCAG TGGGATGGAC CCTCACGGGG AATCCTTACC AAGGTCTCTG 
AGGTACTGTG ACCTGCGTCT GATAAACTCC TCCTGCTTGG TGAGAACAGC GCTGGAGCAG 
GAGCTGGGCC TGGCCGCTTA CTTTGTGAGC AACGAGGTTT CTTTGGAGAA GGCGGCCCGG 
AACGAGCCCT TGGAGAGCGA TGCGGAAAAG CTGAGCAGCA CAGACAACGA GGACGAGGAG 
CTGGTGACGG AAGGCTCCAC CTCGGAGAAG AGGAGCCCGG TGAAGCGGGA GAGGCCCTGC 
TCCCATGATT CGGCGGCGTC CTCCCTCTCC TCGAAAGCTT CCGGCTCGGC ACCGTGCCGC 
GAGTCCTCGG CTCAGCCCGG GGCGCCCTCG CAGGGGGAGC GGGCCCGATC ACCGCAGCCC 
TGCGGCCCGT CGGAGGAGGG CAGGGCCCCC GGGGAGAGGC AGAGGCCCCA CGCTGGCCGG 
GGACCGCCGT CGGTCATCAG CAGGCGCAGC CCCGGGCTGG CCGCCCAGCC GGACTCCGGC 
CCCAGGACCA GCCAGAGGAG CGTCCAGGTG TCGGTCACCC CGTCGTCCTC CCAGCTGTCC 
TCCTCCGGCT CGTCGTCCTC GTCCGGGGCG CTCGTCCCGC AGGCCTCCCG GTGCTCCCTG 
ACCAAGGCCT GCCGCCAGCC GCCCGTCGTC TTCCTGCCCA AGCTGGTGTA TGACATGGTC 
ACGGCCACCG ACAGCAGCGG CCTGCCCAAG GCCGCCTCGC TCCTGCCCTC CCACTCGGTC 
ATGTGGGCCA GCTCCTTCCG CCCGCTGCTC AGCAAGACCA TGACGTCCAC GGAGCAGTCC 
CTCTACTACC GGCAGTGGAC GGTGCCCCGG CCCAGCCACA TGGACTACAG CAACCGCACC 
GAGGGCCGCG TGGACGGCTT CCACCCGCGC CGGCTGCTGC TCAGCGGGCC CCCTCAGGTT 
GGGAAGACAG GTGCTTACCT GCAGTTCCTT AGTATTTTGT CCAGAATGCT CATCCGGCTG 
ACGGAAGTGG ATGTTTATGA TGAAGAAGAG ATCAATATCC ACCTCAGAGA GGAGTCCGAT 
TGGCATTACC TCCAGCTCAG TGACCCCTGG CCAGACCTGG AGCTATTCAA GAAGTTGCCT 
TTTGACTACA TTATTCACGA CCCGAAGTAT GAAGACGCCA GTCTGATCTG TTCGCACCTT 
CAGAGCGTGA AGAGTGAAGC CAGGGGGACG TCCCGGAAGC CGGAGGAGCT CTACGTGCGG 
CGCCAGACAG CGCGGATGAG GCTGTCCAAG TACGCGGCCT ATAACACATA CCACCACTGC 
GAGCAGTGTC ACCACTACAT GGGCTTCCAC CCCCGCTACC AGCTCTATGA GTCCACCCTG 
CACGCCTTTG CCTTCTCTTA CTCCATGCTA GGAGAGGAAA TCCAGCTACA TTTCATCATC 
CCCAAGTCCA AAGAGCACCA CTTTGTCTTC AGCCAACCCG GAGGCCAGCT GGAGAGCATG 
CGGCTGCCCC TGGTCACAGA CAAGAGCCAC GAGTATATAA AAAGTCCGAC GTTCACCCCG 
ACAACCGGCC GTCACGAACA CGGACTCTTT AACCTGTACC ATGCCATGGA TGGTGCCAGC 
CATCTGCACG TGCTGGTCGT CAAGGAGTAT GAAATGGCCA TTTATAAGAA ATACTGGCCC 
AACCACATCA TGCTGGTGCT CCCAAGTATC TTCAACAGCG CTGGAGTTGG CGCCGCCCAC 
TTCCTGATCA AGGAGCTGTC TTACCACAAT CTGGAGCTGG AGAGGAACCG GCAGGAGGAG 
CTGGGGATCA AGCCACAGGA CATCTGGCCT TTCATCGTGA TCTCGGACGA CTCCTGCGTG 
ATGTGGAACG TGGTGGATGT AGACTGTGGC GGAGAAAGAA GCAGGGAGTT TTCCTGGGCA 
GAAAGAAACG TGTCTTTGAA GCACATCATG CAGCACATCG AGGCGTCCCC CAACATCACC 
CACTATGCCC TGATTGGCAT GCGGAAGTGG TCCAGCAAGA CGGGGAGCGC CGAGGTGCGG 
GAGCCCTTCT CCCGCTGCCA CGTGCACGAC TTCATCATTC TGAACGTGGA CCTGACCCAG 
AACGTGCAGT ACCACCAGAA CCGGTTCACG TGTGACGACG TGGACTTCAA CCTGCGGGTG 
CATAGCGCCG GCCTCCTGCT CTGCCGTTTC AACCGCTTTA GCGTGATGAA GAAGCAGATT 
GCCGTGGGTG GGCACAGGTC CTGCCACATC ACGTCCAAGG TATCCGACAA CCCCGTGGCC 
ATCGTGCCGG CCCAGTACAT CTGCGCCCCG GACAGCAAGC ACACGTTCCT CGCGGCGCCC 
GCGCAGCTCC TGCTGGAGAA GTTCCTCCAG CACCACAGTC ACCGATTCTT CCCGCTGTCC 
CTGAGGAACC GCGGCCACCC TGTGCTCTCG GTCGACTGCT ACCTTAACCT GGGATCTCAG 
ATTTCTGTGT GTTATGTGAG CTCCAGGCCC CACTCCCTGA ACATCAGCTG CTCTGACTTC 
GTGTTTAGCG GACTCCTGCT ATACCTCTGT GATTCTTTTG TGGGAGCTAG CTTTTTGAAA 
AAGTTTCATT TTCTGAAAGG TGCCACCCTG TGCGTGATCT GCCAGGACCG GAACTCCCTG 
CGCCAGACGG TGGTCCGCCT CGAGCTGGAA GACGAGTGGC AGTTCCGGCT GCGTGACGAA 
TTCCAGACGG CCAACGCCAA GGAAGACCGG CCGCTCTTTT TTCTGACCGG GCGGCACATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026933714.1
CDS	85..6027
Translation	MRKEHKCTSCTSMFNREGFLPTPLGIESGEQPPSSSLKMGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPVFSQLYLEAEQQLSTLEGGGRGDNEDEEEEGEGALEPSGPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGTLTKGPLICWKGSEFRSRPTSASACSSSLFPPAESSGPLAAFSSEPVPGASPSVPLGAQQAVPASEHPSLATAVGPAVFNGRDSPKHQQLVKNDLSAMPRPSALGILSNSGPPKKRHKGWSPESPSATDAGYAQGGGNRAKYESAGMSCVPQVGSVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLSDVVVSPLLYTCYQNSQSISRAYEHYGASAIQPISEEVQLLLTVYYLVQLADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAQPQPCPPAPSAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIVRACRSPAIDSCVAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDLLGAFFSTLCPEGDIDILLDKFHQENQGHISSSLTASSVTKSASLDAPACTSYNLEPHHIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLSELGLEKEHMTKQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPAVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPASYSNGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPSSGITVGKHFVKQLKLWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNEVSLEKAARNEPLESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERPCSHDSAASSLSSKASGSAPCRESSAQPGAPSQGERARSPQPCGPSEEGRAPGERQRPHAGRGPPSVISRRSPGLAAQPDSGPRTSQRSVQVSVTPSSSQLSSSGSSSSSGALVPQASRCSLTKACRQPPVVFLPKLVYDMVTATDSSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPSHMDYSNRTEGRVDGFHPRRLLLSGPPQVGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINIHLREESDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHLQSVKSEARGTSRKPEELYVRRQTARMRLSKYAAYNTYHHCEQCHHYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDCGGERSREFSWAERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSAEVREPFSRCHVHDFIILNVDLTQNVQYHQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSCHITSKVSDNPVAIVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLRNRGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_027077913.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027077913.1

ORF Insert Sequence:

ATGAGGAAAG AACACAAGTG CACATCTTGT ACTTCGATGT TCAACAGGGA GGGCTTCCTC 
CCGACCCCGC TCGGAATAGA AAGCGGTGAG CAGCCTCCGT CGTCCTCTCT AAAGATGGGA 
AACTCGTACG CCGGCCAGCT GAAGACGACG CGGTTCGAAG AGGTCTTGCA CAACTCCATC 
GAGGCTTCGC TGCGGTCCAA CAGCCTGGTG CCCAGGCCCG TCTTCTCCCA GTTGTATCTA 
GAAGCCGAAC AGCAGCTCTC CACGCTAGAA GGTGGAGGCC GAGGGGACAA TGAGGATGAG 
GAAGAAGAGG GGGAAGGGGC GCTGGAGCCC AGCGGTCCCC CGAATCCCTA CCAGATGCAC 
CCTCCGCCGG AAGGCTGCTG CACCACAGAC GGGTTCTGCC AGGCGGGGAA GGACCTGCGT 
CTCGTCTCCA TTTCCAGTGA GCCCATAGAC GTCCCTGCAG GCTTTCTCCT CGTGGGGGCC 
AAGTCCCCCA GCCTGCCAGA CCATCTCCTG GTGTGTGCTG TGGACAAAAG GTTCTTGCCG 
GATGACAATG GGCACAACGC TCTTCTTGGT TTCTCTGGGA ATTGCGTTGG CTGTGGAAAG 
AAAGGCTTCT GCTACTTCAC AGAATTCTCT AATCACATAA ATCTCAAGTT GACCACTCAG 
CCCAAGAAGC AGAAACACTT GAAGTATTAC CTGGTCCGCA ACGCACAAGG GACTCTAACC 
AAAGGACCTC TCATCTGTTG GAAAGGCTCA GAGTTCAGAA GCCGGCCGAC CTCCGCCAGT 
GCCTGTTCCA GCTCACTCTT CCCACCAGCG GAGAGCTCCG GGCCTCTGGC TGCCTTCTCC 
AGCGAGCCCG TTCCCGGAGC CAGCCCGAGC GTCCCCCTGG GAGCCCAGCA GGCAGTGCCA 
GCCTCTGAGC ACCCCTCACT TGCTACGGCC GTGGGTCCAG CTGTTTTCAA TGGCAGAGAT 
TCTCCGAAGC ACCAACAGCT GGTGAAGAAC GACCTATCGG CCATGCCACG GCCCTCGGCA 
TTAGGTATCT TATCAAACTC TGGGCCCCCG AAGAAACGCC ACAAAGGGTG GTCCCCTGAA 
TCTCCATCAG CGACAGACGC CGGCTACGCC CAGGGTGGTG GGAACAGAGC TAAGTACGAG 
AGCGCAGGCA TGTCCTGCGT GCCACAGGTT GGCTCGGTGG GACCAGCGTC GGTCACCTTC 
CCCGTTGTGG CCTCTGGAGA ACCAGTGTCC GTCCCTGACA ACTTGCTGAA AATATGCAAG 
GCCAAGCCGG TGATATTTAA AGGCCACGGG AACTTCCCTT ACCTCTGCGG GAACCTGAGT 
GACGTCGTCG TGAGCCCCCT CCTGTACACG TGCTACCAGA ACTCTCAGTC CATCTCCCGG 
GCGTATGAGC ACTACGGGGC CTCGGCGATA CAGCCCATCT CCGAGGAGGT GCAGCTCCTA 
CTGACCGTCT ACTATCTCGT CCAGCTGGCG GACCAGGTGC CCCTGATGGA GGACCTGGAG 
CAGATCTTCC TGCGCTCGTG GCGCGAATCG CACCTGACCG AGATCCGGCA GTACCAGCAG 
GCGCAGCCGC AGCCCTGCCC GCCCGCGCCC AGCGCCGTGG CCCCCGTCAC CTCGGCCCAG 
CTGCCCTGGC TCGCCGGCCT GGCCGCCAGC TCCTGCAACG ACAGCGTGCA CATCATCGAG 
TGCACCTACT CCCTGGCCGA GGGCCTCTCC GAGATGTTCC GGCTGCTCAT CGAGGGCAAG 
CTCGCCAAGA CCAACTACGT GGTGATCGTC CGCGCGTGCC GCAGCCCCGC CATCGACTCC 
TGCGTCGCCG TCACGGGAAA ATACCAAGCC CGAATTCTTT CTGAGAGCCT CCTCACCCCA 
GCGGAGTACC AGAAAGAAGT CAATTACGAG CTGGTTACCG GGAAAGTGGA CTTGTTGGGG 
GCGTTTTTCA GCACCCTCTG TCCAGAGGGG GACATTGACA TTTTGCTGGA CAAATTTCAT 
CAGGAAAATC AAGGCCATAT TTCGTCGTCA CTTACTGCCT CCTCTGTCAC CAAATCAGCA 
TCCCTGGATG CACCGGCATG CACAAGTTAT AATCTGGAGC CCCACCACAT CCGGCCTTTC 
CAGCTGGCCG TGGCTCAGAA GCTCCTCTCC CATGTGTGTT CAATTGCGGA TTCCAGCACC 
CAGAATTTGG ACTTAGGATC CTTCGAGAAG GTGGACTTTC TGATTTGTAT TCCACCCTCA 
GAAGTGACCT ATCAGCAAAC CCTGTTCCAC GTCTGGCATT CAGGGGTTTT GTCAGAGCTC 
GGCTTGGAAA AGGAGCACAT GACCAAGCAG AGGGTTGAAC AGTATGTTTT GAAGCTTGAT 
GCTGAAGCGC AGACAAAATT TAAGTCCTTT CTGCAAAACT CCTTTCAGAA TCCGCATACG 
CTCTTTGTCC TGATCCACGA CCACGCCCAC TGGGACCTCG TGAGTAGCGC TGTTCATAAT 
CTCTATTCCC AAAGTGACCC GGCTGTGGGA TTGGTGGACC GGTTGCTGAA CTGCAGAGAG 
GTGAAGGAGG CCCCGAACAT CGTGACGCTT CACGTGACTT CCTTCCCTTA CGCTCTGCAG 
ACACAGCACA CCCTCATCAG CCCTTACAAT GAGATCCACT GGCCCGCCTC CTACAGCAAT 
GGGGTGGACT TATATCCTGA GAACAAGAAG TACTTCGGGC TGTCAGAGTT CATTGAGTCC 
ACCCTTTCGG GACACAGCCT CCCTTTGCTC AGATATGACA GCTCCTTTGA GGCCATGGTC 
ACGGCATTAG GAAAAAGGTT CCCCCGCCTG CACAGCGCGG TGATCAGGAC CTTTGTTCTC 
GTGCAGCACT ACGCGGCCGC CATGATGGCC GTGAGCGGCC TGTCGCAGAT GAAGAACTAC 
ACGTCGGTGG AGACGCTGGA GATCACCCAG AACCTCATCA ACTCCCCCAA GCAGTGCCCC 
TGCGGCCACG GGCTCATGGT CCTGCTGCGG GTGCCCTGCT CCCCGCTGGC GGCGGTGGCC 
TACGAGCGGC TGGCCCACGT GCGGGCACGC CTGGCCCTGG AGGAGCACTT TGAGATCATC 
CTGGGCAACC CCAGCTCCGG CATCACCGTC GGGAAGCACT TCGTGAAGCA ACTCAAGCTG 
TGGCAGAAGA TCGAGGACGC CGAGTGGAGA CCTCAGACCT ACCTGGAGCT GGAGGGCCTG 
CCCTGCATCC TGATCTTCAG TGGGATGGAC CCTCACGGGG AATCCTTACC AAGGTCTCTG 
AGGTACTGTG ACCTGCGTCT GATAAACTCC TCCTGCTTGG TGAGAACAGC GCTGGAGCAG 
GAGCTGGGCC TGGCCGCTTA CTTTGTGAGC AACGAGGTTT CTTTGGAGAA GGCGGCCCGG 
AACGAGCCCT TGGAGAGCGA TGCGGAAAAG CTGAGCAGCA CAGACAACGA GGACGAGGAG 
CTGGTGACGG AAGGCTCCAC CTCGGAGAAG AGGAGCCCGG TGAAGCGGGA GAGGCCCTGC 
TCCCATGATT CGGCGGCGTC CTCCCTCTCC TCGAAAGCTT CCGGCTCGGC ACCGTGCCGC 
GAGTCCTCGG CTCAGCCCGG GGCGCCCTCG CAGGGGGAGC GGGCCCGATC ACCGCAGCCC 
TGCGGCCCGT CGGAGGAGGG CAGGGCCCCC GGGGAGAGGC AGAGGCCCCA CGCTGGCCGG 
GGACCGCCGT CGGTCATCAG CAGGCGCAGC CCCGGGCTGG CCGCCCAGCC GGACTCCGGC 
CCCAGGACCA GCCAGAGGAG CGTCCAGGTG TCGGTCACCC CGTCGTCCTC CCAGCTGTCC 
TCCTCCGGCT CGTCGTCCTC GTCCGGGGCG CTCGTCCCGC AGGCCTCCCG GTGCTCCCTG 
ACCAAGGCCT GCCGCCAGCC GCCCGTCGTC TTCCTGCCCA AGCTGGTGTA TGACATGGTC 
ACGGCCACCG ACAGCAGCGG CCTGCCCAAG GCCGCCTCGC TCCTGCCCTC CCACTCGGTC 
ATGTGGGCCA GCTCCTTCCG CCCGCTGCTC AGCAAGACCA TGACGTCCAC GGAGCAGTCC 
CTCTACTACC GGCAGTGGAC GGTGCCCCGG CCCAGCCACA TGGACTACAG CAACCGCACC 
GAGGGCCGCG TGGACGGCTT CCACCCGCGC CGGCTGCTGC TCAGCGGGCC CCCTCAGGTT 
GGGAAGACAG GTGCTTACCT GCAGTTCCTT AGTATTTTGT CCAGAATGCT CATCCGGCTG 
ACGGAAGTGG ATGTTTATGA TGAAGAAGAG ATCAATATCC ACCTCAGAGA GGAGTCCGAT 
TGGCATTACC TCCAGCTCAG TGACCCCTGG CCAGACCTGG AGCTATTCAA GAAGTTGCCT 
TTTGACTACA TTATTCACGA CCCGAAGTAT GAAGACGCCA GTCTGATCTG TTCGCACCTT 
CAGAGCGTGA AGAGTGAAGC CAGGGGGACG TCCCGGAAGC CGGAGGAGCT CTACGTGCGG 
CGCCAGACAG CGCGGATGAG GCTGTCCAAG TACGCGGCCT ATAACACATA CCACCACTGC 
GAGCAGTGTC ACCACTACAT GGGCTTCCAC CCCCGCTACC AGCTCTATGA GTCCACCCTG 
CACGCCTTTG CCTTCTCTTA CTCCATGCTA GGAGAGGAAA TCCAGCTACA TTTCATCATC 
CCCAAGTCCA AAGAGCACCA CTTTGTCTTC AGCCAACCCG GAGGCCAGCT GGAGAGCATG 
CGGCTGCCCC TGGTCACAGA CAAGAGCCAC GAGTATATAA AAAGTCCGAC GTTCACCCCG 
ACAACCGGCC GTCACGAACA CGGACTCTTT AACCTGTACC ATGCCATGGA TGGTGCCAGC 
CATCTGCACG TGCTGGTCGT CAAGGAGTAT GAAATGGCCA TTTATAAGAA ATACTGGCCC 
AACCACATCA TGCTGGTGCT CCCAAGTATC TTCAACAGCG CTGGAGTTGG CGCCGCCCAC 
TTCCTGATCA AGGAGCTGTC TTACCACAAT CTGGAGCTGG AGAGGAACCG GCAGGAGGAG 
CTGGGGATCA AGCCACAGGA CATCTGGCCT TTCATCGTGA TCTCGGACGA CTCCTGCGTG 
ATGTGGAACG TGGTGGATGT AGACTGTGGC GGAGAAAGAA GCAGGGAGTT TTCCTGGGCA 
GAAAGAAACG TGTCTTTGAA GCACATCATG CAGCACATCG AGGCGTCCCC CAACATCACC 
CACTATGCCC TGATTGGCAT GCGGAAGTGG TCCAGCAAGA CGGGGAGCGC CGAGGTGCGG 
GAGCCCTTCT CCCGCTGCCA CGTGCACGAC TTCATCATTC TGAACGTGGA CCTGACCCAG 
AACGTGCAGT ACCACCAGAA CCGGTTCACG TGTGACGACG TGGACTTCAA CCTGCGGGTG 
CATAGCGCCG GCCTCCTGCT CTGCCGTTTC AACCGCTTTA GCGTGATGAA GAAGCAGATT 
GCCGTGGGTG GGCACAGGTC CTGCCACATC ACGTCCAAGG TATCCGACAA CCCCGTGGCC 
ATCGTGCCGG CCCAGTACAT CTGCGCCCCG GACAGCAAGC ACACGTTCCT CGCGGCGCCC 
GCGCAGCTCC TGCTGGAGAA GTTCCTCCAG CACCACAGTC ACCGATTCTT CCCGCTGTCC 
CTGAGGAACC GCGGCCACCC TGTGCTCTCG GTCGACTGCT ACCTTAACCT GGGATCTCAG 
ATTTCTGTGT GTTATGTGAG CTCCAGGCCC CACTCCCTGA ACATCAGCTG CTCTGACTTC 
GTGTTTAGCG GACTCCTGCT ATACCTCTGT GATTCTTTTG TGGGAGCTAG CTTTTTGAAA 
AAGTTTCATT TTCTGAAAGG TGCCACCCTG TGCGTGATCT GCCAGGACCG GAACTCCCTG 
CGCCAGACGG TGGTCCGCCT CGAGCTGGAA GACGAGTGGC AGTTCCGGCT GCGTGACGAA 
TTCCAGACGG CCAACGCCAA GGAAGACCGG CCGCTCTTTT TTCTGACCGG GCGGCACATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_027077915.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026933716.1 Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X3, mRNA.

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CloneID	OAc233924
Clone ID Related Accession (Same CDS sequence)	XM_027077915.1
Accession Version	XM_027077915.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	protein GREB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834765.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAAG AACACAAGTG CACATCTTGT ACTTCGATGT TCAACAGGGA GGGCTTCCTC 
CCGACCCCGC TCGGAATAGA AAGCGGTGAG CAGCCTCCGT CGTCCTCTCT AAAGATGGGA 
AACTCGTACG CCGGCCAGCT GAAGACGACG CGGTTCGAAG AGGTCTTGCA CAACTCCATC 
GAGGCTTCGC TGCGGTCCAA CAGCCTGGTG CCCAGGCCCG TCTTCTCCCA GTTGTATCTA 
GAAGCCGAAC AGCAGCTCTC CACGCTAGAA GGTGGAGGCC GAGGGGACAA TGAGGATGAG 
GAAGAAGAGG GGGAAGGGGC GCTGGAGCCC AGCGGTCCCC CGAATCCCTA CCAGATGCAC 
CCTCCGCCGG AAGGCTGCTG CACCACAGAC GGGTTCTGCC AGGCGGGGAA GGACCTGCGT 
CTCGTCTCCA TTTCCAGTGA GCCCATAGAC GTCCCTGCAG GCTTTCTCCT CGTGGGGGCC 
AAGTCCCCCA GCCTGCCAGA CCATCTCCTG GTGTGTGCTG TGGACAAAAG GTTCTTGCCG 
GATGACAATG GGCACAACGC TCTTCTTGGT TTCTCTGGGA ATTGCGTTGG CTGTGGAAAG 
AAAGGCTTCT GCTACTTCAC AGAATTCTCT AATCACATAA ATCTCAAGTT GACCACTCAG 
CCCAAGAAGC AGAAACACTT GAAGTATTAC CTGGTCCGCA ACGCACAAGG GACTCTAACC 
AAAGGACCTC TCATCTGTTG GAAAGGCTCA GAGTTCAGAA GCCGGCCGAC CTCCGCCAGT 
GCCTGTTCCA GCTCACTCTT CCCACCAGCG GAGAGCTCCG GGCCTCTGGC TGCCTTCTCC 
AGCGAGCCCG TTCCCGGAGC CAGCCCGAGC GTCCCCCTGG GAGCCCAGCA GGCAGTGCCA 
GCCTCTGAGC ACCCCTCACT TGCTACGGCC GTGGGTCCAG CTGTTTTCAA TGGCAGAGAT 
TCTCCGAAGC ACCAACAGCT GGTGAAGAAC GACCTATCGG CCATGCCACG GCCCTCGGCA 
TTAGGTATCT TATCAAACTC TGGGCCCCCG AAGAAACGCC ACAAAGGGTG GTCCCCTGAA 
TCTCCATCAG CGACAGACGC CGGCTACGCC CAGGGTGGTG GGAACAGAGC TAAGTACGAG 
AGCGCAGGCA TGTCCTGCGT GCCACAGGTT GGCTCGGTGG GACCAGCGTC GGTCACCTTC 
CCCGTTGTGG CCTCTGGAGA ACCAGTGTCC GTCCCTGACA ACTTGCTGAA AATATGCAAG 
GCCAAGCCGG TGATATTTAA AGGCCACGGG AACTTCCCTT ACCTCTGCGG GAACCTGAGT 
GACGTCGTCG TGAGCCCCCT CCTGTACACG TGCTACCAGA ACTCTCAGTC CATCTCCCGG 
GCGTATGAGC ACTACGGGGC CTCGGCGATA CAGCCCATCT CCGAGGAGGT GCAGCTCCTA 
CTGACCGTCT ACTATCTCGT CCAGCTGGCA GCGGACCAGG TGCCCCTGAT GGAGGACCTG 
GAGCAGATCT TCCTGCGCTC GTGGCGCGAA TCGCACCTGA CCGAGATCCG GCAGTACCAG 
CAGGCGCAGC CGCAGCCCTG CCCGCCCGCG CCCAGCGCCG TGGCCCCCGT CACCTCGGCC 
CAGCTGCCCT GGCTCGCCGG CCTGGCCGCC AGCTCCTGCA ACGACAGCGT GCACATCATC 
GAGTGCACCT ACTCCCTGGC CGAGGGCCTC TCCGAGATGT TCCGGCTGCT CATCGAGGGC 
AAGCTCGCCA AGACCAACTA CGTGGTGATC GTCCGCGCGT GCCGCAGCCC CGCCATCGAC 
TCCTGCGTCG CCGTCACGGG AAAATACCAA GCCCGAATTC TTTCTGAGAG CCTCCTCACC 
CCAGCGGAGT ACCAGAAAGA AGTCAATTAC GAGCTGGTTA CCGGGAAAGT GGACTTGTTG 
GGGGCGTTTT TCAGCACCCT CTGTCCAGAG GGGGACATTG ACATTTTGCT GGACAAATTT 
CATCAGGAAA ATCAAGGCCA TATTTCGTCG TCACTTACTG CCTCCTCTGT CACCAAATCA 
GCATCCCTGG ATGCACCGGC ATGCACAAGT TATAATCTGG AGCCCCACCA CATCCGGCCT 
TTCCAGCTGG CCGTGGCTCA GAAGCTCCTC TCCCATGTGT GTTCAATTGC GGATTCCAGC 
ACCCAGAATT TGGACTTAGG ATCCTTCGAG AAGGTGGACT TTCTGATTTG TATTCCACCC 
TCAGAAGTGA CCTATCAGCA AACCCTGTTC CACGTCTGGC ATTCAGGGGT TTTGTCAGAG 
CTCGGCTTGG AAAAGGAGCA CATGACCAAG CAGAGGGTTG AACAGTATGT TTTGAAGCTT 
GATGCTGAAG CGCAGACAAA ATTTAAGTCC TTTCTGCAAA ACTCCTTTCA GAATCCGCAT 
ACGCTCTTTG TCCTGATCCA CGACCACGCC CACTGGGACC TCGTGAGCGC TGTTCATAAT 
CTCTATTCCC AAAGTGACCC GGCTGTGGGA TTGGTGGACC GGTTGCTGAA CTGCAGAGAG 
GTGAAGGAGG CCCCGAACAT CGTGACGCTT CACGTGACTT CCTTCCCTTA CGCTCTGCAG 
ACACAGCACA CCCTCATCAG CCCTTACAAT GAGATCCACT GGCCCGCCTC CTACAGCAAT 
GGGGTGGACT TATATCCTGA GAACAAGAAG TACTTCGGGC TGTCAGAGTT CATTGAGTCC 
ACCCTTTCGG GACACAGCCT CCCTTTGCTC AGATATGACA GCTCCTTTGA GGCCATGGTC 
ACGGCATTAG GAAAAAGGTT CCCCCGCCTG CACAGCGCGG TGATCAGGAC CTTTGTTCTC 
GTGCAGCACT ACGCGGCCGC CATGATGGCC GTGAGCGGCC TGTCGCAGAT GAAGAACTAC 
ACGTCGGTGG AGACGCTGGA GATCACCCAG AACCTCATCA ACTCCCCCAA GCAGTGCCCC 
TGCGGCCACG GGCTCATGGT CCTGCTGCGG GTGCCCTGCT CCCCGCTGGC GGCGGTGGCC 
TACGAGCGGC TGGCCCACGT GCGGGCACGC CTGGCCCTGG AGGAGCACTT TGAGATCATC 
CTGGGCAACC CCAGCTCCGG CATCACCGTC GGGAAGCACT TCGTGAAGCA ACTCAAGCTG 
TGGCAGAAGA TCGAGGACGC CGAGTGGAGA CCTCAGACCT ACCTGGAGCT GGAGGGCCTG 
CCCTGCATCC TGATCTTCAG TGGGATGGAC CCTCACGGGG AATCCTTACC AAGGTCTCTG 
AGGTACTGTG ACCTGCGTCT GATAAACTCC TCCTGCTTGG TGAGAACAGC GCTGGAGCAG 
GAGCTGGGCC TGGCCGCTTA CTTTGTGAGC AACGAGGTTT CTTTGGAGAA GGCGGCCCGG 
AACGAGCCCT TGGAGAGCGA TGCGGAAAAG CTGAGCAGCA CAGACAACGA GGACGAGGAG 
CTGGTGACGG AAGGCTCCAC CTCGGAGAAG AGGAGCCCGG TGAAGCGGGA GAGGCCCTGC 
TCCCATGATT CGGCGGCGTC CTCCCTCTCC TCGAAAGCTT CCGGCTCGGC ACCGTGCCGC 
GAGTCCTCGG CTCAGCCCGG GGCGCCCTCG CAGGGGGAGC GGGCCCGATC ACCGCAGCCC 
TGCGGCCCGT CGGAGGAGGG CAGGGCCCCC GGGGAGAGGC AGAGGCCCCA CGCTGGCCGG 
GGACCGCCGT CGGTCATCAG CAGGCGCAGC CCCGGGCTGG CCGCCCAGCC GGACTCCGGC 
CCCAGGACCA GCCAGAGGAG CGTCCAGGTG TCGGTCACCC CGTCGTCCTC CCAGCTGTCC 
TCCTCCGGCT CGTCGTCCTC GTCCGGGGCG CTCGTCCCGC AGGCCTCCCG GTGCTCCCTG 
ACCAAGGCCT GCCGCCAGCC GCCCGTCGTC TTCCTGCCCA AGCTGGTGTA TGACATGGTC 
ACGGCCACCG ACAGCAGCGG CCTGCCCAAG GCCGCCTCGC TCCTGCCCTC CCACTCGGTC 
ATGTGGGCCA GCTCCTTCCG CCCGCTGCTC AGCAAGACCA TGACGTCCAC GGAGCAGTCC 
CTCTACTACC GGCAGTGGAC GGTGCCCCGG CCCAGCCACA TGGACTACAG CAACCGCACC 
GAGGGCCGCG TGGACGGCTT CCACCCGCGC CGGCTGCTGC TCAGCGGGCC CCCTCAGGTT 
GGGAAGACAG GTGCTTACCT GCAGTTCCTT AGTATTTTGT CCAGAATGCT CATCCGGCTG 
ACGGAAGTGG ATGTTTATGA TGAAGAAGAG ATCAATATCC ACCTCAGAGA GGAGTCCGAT 
TGGCATTACC TCCAGCTCAG TGACCCCTGG CCAGACCTGG AGCTATTCAA GAAGTTGCCT 
TTTGACTACA TTATTCACGA CCCGAAGTAT GAAGACGCCA GTCTGATCTG TTCGCACCTT 
CAGAGCGTGA AGAGTGAAGC CAGGGGGACG TCCCGGAAGC CGGAGGAGCT CTACGTGCGG 
CGCCAGACAG CGCGGATGAG GCTGTCCAAG TACGCGGCCT ATAACACATA CCACCACTGC 
GAGCAGTGTC ACCACTACAT GGGCTTCCAC CCCCGCTACC AGCTCTATGA GTCCACCCTG 
CACGCCTTTG CCTTCTCTTA CTCCATGCTA GGAGAGGAAA TCCAGCTACA TTTCATCATC 
CCCAAGTCCA AAGAGCACCA CTTTGTCTTC AGCCAACCCG GAGGCCAGCT GGAGAGCATG 
CGGCTGCCCC TGGTCACAGA CAAGAGCCAC GAGTATATAA AAAGTCCGAC GTTCACCCCG 
ACAACCGGCC GTCACGAACA CGGACTCTTT AACCTGTACC ATGCCATGGA TGGTGCCAGC 
CATCTGCACG TGCTGGTCGT CAAGGAGTAT GAAATGGCCA TTTATAAGAA ATACTGGCCC 
AACCACATCA TGCTGGTGCT CCCAAGTATC TTCAACAGCG CTGGAGTTGG CGCCGCCCAC 
TTCCTGATCA AGGAGCTGTC TTACCACAAT CTGGAGCTGG AGAGGAACCG GCAGGAGGAG 
CTGGGGATCA AGCCACAGGA CATCTGGCCT TTCATCGTGA TCTCGGACGA CTCCTGCGTG 
ATGTGGAACG TGGTGGATGT AGACTGTGGC GGAGAAAGAA GCAGGGAGTT TTCCTGGGCA 
GAAAGAAACG TGTCTTTGAA GCACATCATG CAGCACATCG AGGCGTCCCC CAACATCACC 
CACTATGCCC TGATTGGCAT GCGGAAGTGG TCCAGCAAGA CGGGGAGCGC CGAGGTGCGG 
GAGCCCTTCT CCCGCTGCCA CGTGCACGAC TTCATCATTC TGAACGTGGA CCTGACCCAG 
AACGTGCAGT ACCACCAGAA CCGGTTCACG TGTGACGACG TGGACTTCAA CCTGCGGGTG 
CATAGCGCCG GCCTCCTGCT CTGCCGTTTC AACCGCTTTA GCGTGATGAA GAAGCAGATT 
GCCGTGGGTG GGCACAGGTC CTGCCACATC ACGTCCAAGG TATCCGACAA CCCCGTGGCC 
ATCGTGCCGG CCCAGTACAT CTGCGCCCCG GACAGCAAGC ACACGTTCCT CGCGGCGCCC 
GCGCAGCTCC TGCTGGAGAA GTTCCTCCAG CACCACAGTC ACCGATTCTT CCCGCTGTCC 
CTGAGGAACC GCGGCCACCC TGTGCTCTCG GTCGACTGCT ACCTTAACCT GGGATCTCAG 
ATTTCTGTGT GTTATGTGAG CTCCAGGCCC CACTCCCTGA ACATCAGCTG CTCTGACTTC 
GTGTTTAGCG GACTCCTGCT ATACCTCTGT GATTCTTTTG TGGGAGCTAG CTTTTTGAAA 
AAGTTTCATT TTCTGAAAGG TGCCACCCTG TGCGTGATCT GCCAGGACCG GAACTCCCTG 
CGCCAGACGG TGGTCCGCCT CGAGCTGGAA GACGAGTGGC AGTTCCGGCT GCGTGACGAA 
TTCCAGACGG CCAACGCCAA GGAAGACCGG CCGCTCTTTT TTCTGACCGG GCGGCACATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026933716.1
CDS	85..6027
Translation	MRKEHKCTSCTSMFNREGFLPTPLGIESGEQPPSSSLKMGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPVFSQLYLEAEQQLSTLEGGGRGDNEDEEEEGEGALEPSGPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGTLTKGPLICWKGSEFRSRPTSASACSSSLFPPAESSGPLAAFSSEPVPGASPSVPLGAQQAVPASEHPSLATAVGPAVFNGRDSPKHQQLVKNDLSAMPRPSALGILSNSGPPKKRHKGWSPESPSATDAGYAQGGGNRAKYESAGMSCVPQVGSVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLSDVVVSPLLYTCYQNSQSISRAYEHYGASAIQPISEEVQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAQPQPCPPAPSAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIVRACRSPAIDSCVAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDLLGAFFSTLCPEGDIDILLDKFHQENQGHISSSLTASSVTKSASLDAPACTSYNLEPHHIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLSELGLEKEHMTKQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSAVHNLYSQSDPAVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPASYSNGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPSSGITVGKHFVKQLKLWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNEVSLEKAARNEPLESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERPCSHDSAASSLSSKASGSAPCRESSAQPGAPSQGERARSPQPCGPSEEGRAPGERQRPHAGRGPPSVISRRSPGLAAQPDSGPRTSQRSVQVSVTPSSSQLSSSGSSSSSGALVPQASRCSLTKACRQPPVVFLPKLVYDMVTATDSSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPSHMDYSNRTEGRVDGFHPRRLLLSGPPQVGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINIHLREESDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHLQSVKSEARGTSRKPEELYVRRQTARMRLSKYAAYNTYHHCEQCHHYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDCGGERSREFSWAERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSAEVREPFSRCHVHDFIILNVDLTQNVQYHQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSCHITSKVSDNPVAIVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLRNRGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_027077915.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027077915.1

ORF Insert Sequence:

ATGAGGAAAG AACACAAGTG CACATCTTGT ACTTCGATGT TCAACAGGGA GGGCTTCCTC 
CCGACCCCGC TCGGAATAGA AAGCGGTGAG CAGCCTCCGT CGTCCTCTCT AAAGATGGGA 
AACTCGTACG CCGGCCAGCT GAAGACGACG CGGTTCGAAG AGGTCTTGCA CAACTCCATC 
GAGGCTTCGC TGCGGTCCAA CAGCCTGGTG CCCAGGCCCG TCTTCTCCCA GTTGTATCTA 
GAAGCCGAAC AGCAGCTCTC CACGCTAGAA GGTGGAGGCC GAGGGGACAA TGAGGATGAG 
GAAGAAGAGG GGGAAGGGGC GCTGGAGCCC AGCGGTCCCC CGAATCCCTA CCAGATGCAC 
CCTCCGCCGG AAGGCTGCTG CACCACAGAC GGGTTCTGCC AGGCGGGGAA GGACCTGCGT 
CTCGTCTCCA TTTCCAGTGA GCCCATAGAC GTCCCTGCAG GCTTTCTCCT CGTGGGGGCC 
AAGTCCCCCA GCCTGCCAGA CCATCTCCTG GTGTGTGCTG TGGACAAAAG GTTCTTGCCG 
GATGACAATG GGCACAACGC TCTTCTTGGT TTCTCTGGGA ATTGCGTTGG CTGTGGAAAG 
AAAGGCTTCT GCTACTTCAC AGAATTCTCT AATCACATAA ATCTCAAGTT GACCACTCAG 
CCCAAGAAGC AGAAACACTT GAAGTATTAC CTGGTCCGCA ACGCACAAGG GACTCTAACC 
AAAGGACCTC TCATCTGTTG GAAAGGCTCA GAGTTCAGAA GCCGGCCGAC CTCCGCCAGT 
GCCTGTTCCA GCTCACTCTT CCCACCAGCG GAGAGCTCCG GGCCTCTGGC TGCCTTCTCC 
AGCGAGCCCG TTCCCGGAGC CAGCCCGAGC GTCCCCCTGG GAGCCCAGCA GGCAGTGCCA 
GCCTCTGAGC ACCCCTCACT TGCTACGGCC GTGGGTCCAG CTGTTTTCAA TGGCAGAGAT 
TCTCCGAAGC ACCAACAGCT GGTGAAGAAC GACCTATCGG CCATGCCACG GCCCTCGGCA 
TTAGGTATCT TATCAAACTC TGGGCCCCCG AAGAAACGCC ACAAAGGGTG GTCCCCTGAA 
TCTCCATCAG CGACAGACGC CGGCTACGCC CAGGGTGGTG GGAACAGAGC TAAGTACGAG 
AGCGCAGGCA TGTCCTGCGT GCCACAGGTT GGCTCGGTGG GACCAGCGTC GGTCACCTTC 
CCCGTTGTGG CCTCTGGAGA ACCAGTGTCC GTCCCTGACA ACTTGCTGAA AATATGCAAG 
GCCAAGCCGG TGATATTTAA AGGCCACGGG AACTTCCCTT ACCTCTGCGG GAACCTGAGT 
GACGTCGTCG TGAGCCCCCT CCTGTACACG TGCTACCAGA ACTCTCAGTC CATCTCCCGG 
GCGTATGAGC ACTACGGGGC CTCGGCGATA CAGCCCATCT CCGAGGAGGT GCAGCTCCTA 
CTGACCGTCT ACTATCTCGT CCAGCTGGCA GCGGACCAGG TGCCCCTGAT GGAGGACCTG 
GAGCAGATCT TCCTGCGCTC GTGGCGCGAA TCGCACCTGA CCGAGATCCG GCAGTACCAG 
CAGGCGCAGC CGCAGCCCTG CCCGCCCGCG CCCAGCGCCG TGGCCCCCGT CACCTCGGCC 
CAGCTGCCCT GGCTCGCCGG CCTGGCCGCC AGCTCCTGCA ACGACAGCGT GCACATCATC 
GAGTGCACCT ACTCCCTGGC CGAGGGCCTC TCCGAGATGT TCCGGCTGCT CATCGAGGGC 
AAGCTCGCCA AGACCAACTA CGTGGTGATC GTCCGCGCGT GCCGCAGCCC CGCCATCGAC 
TCCTGCGTCG CCGTCACGGG AAAATACCAA GCCCGAATTC TTTCTGAGAG CCTCCTCACC 
CCAGCGGAGT ACCAGAAAGA AGTCAATTAC GAGCTGGTTA CCGGGAAAGT GGACTTGTTG 
GGGGCGTTTT TCAGCACCCT CTGTCCAGAG GGGGACATTG ACATTTTGCT GGACAAATTT 
CATCAGGAAA ATCAAGGCCA TATTTCGTCG TCACTTACTG CCTCCTCTGT CACCAAATCA 
GCATCCCTGG ATGCACCGGC ATGCACAAGT TATAATCTGG AGCCCCACCA CATCCGGCCT 
TTCCAGCTGG CCGTGGCTCA GAAGCTCCTC TCCCATGTGT GTTCAATTGC GGATTCCAGC 
ACCCAGAATT TGGACTTAGG ATCCTTCGAG AAGGTGGACT TTCTGATTTG TATTCCACCC 
TCAGAAGTGA CCTATCAGCA AACCCTGTTC CACGTCTGGC ATTCAGGGGT TTTGTCAGAG 
CTCGGCTTGG AAAAGGAGCA CATGACCAAG CAGAGGGTTG AACAGTATGT TTTGAAGCTT 
GATGCTGAAG CGCAGACAAA ATTTAAGTCC TTTCTGCAAA ACTCCTTTCA GAATCCGCAT 
ACGCTCTTTG TCCTGATCCA CGACCACGCC CACTGGGACC TCGTGAGCGC TGTTCATAAT 
CTCTATTCCC AAAGTGACCC GGCTGTGGGA TTGGTGGACC GGTTGCTGAA CTGCAGAGAG 
GTGAAGGAGG CCCCGAACAT CGTGACGCTT CACGTGACTT CCTTCCCTTA CGCTCTGCAG 
ACACAGCACA CCCTCATCAG CCCTTACAAT GAGATCCACT GGCCCGCCTC CTACAGCAAT 
GGGGTGGACT TATATCCTGA GAACAAGAAG TACTTCGGGC TGTCAGAGTT CATTGAGTCC 
ACCCTTTCGG GACACAGCCT CCCTTTGCTC AGATATGACA GCTCCTTTGA GGCCATGGTC 
ACGGCATTAG GAAAAAGGTT CCCCCGCCTG CACAGCGCGG TGATCAGGAC CTTTGTTCTC 
GTGCAGCACT ACGCGGCCGC CATGATGGCC GTGAGCGGCC TGTCGCAGAT GAAGAACTAC 
ACGTCGGTGG AGACGCTGGA GATCACCCAG AACCTCATCA ACTCCCCCAA GCAGTGCCCC 
TGCGGCCACG GGCTCATGGT CCTGCTGCGG GTGCCCTGCT CCCCGCTGGC GGCGGTGGCC 
TACGAGCGGC TGGCCCACGT GCGGGCACGC CTGGCCCTGG AGGAGCACTT TGAGATCATC 
CTGGGCAACC CCAGCTCCGG CATCACCGTC GGGAAGCACT TCGTGAAGCA ACTCAAGCTG 
TGGCAGAAGA TCGAGGACGC CGAGTGGAGA CCTCAGACCT ACCTGGAGCT GGAGGGCCTG 
CCCTGCATCC TGATCTTCAG TGGGATGGAC CCTCACGGGG AATCCTTACC AAGGTCTCTG 
AGGTACTGTG ACCTGCGTCT GATAAACTCC TCCTGCTTGG TGAGAACAGC GCTGGAGCAG 
GAGCTGGGCC TGGCCGCTTA CTTTGTGAGC AACGAGGTTT CTTTGGAGAA GGCGGCCCGG 
AACGAGCCCT TGGAGAGCGA TGCGGAAAAG CTGAGCAGCA CAGACAACGA GGACGAGGAG 
CTGGTGACGG AAGGCTCCAC CTCGGAGAAG AGGAGCCCGG TGAAGCGGGA GAGGCCCTGC 
TCCCATGATT CGGCGGCGTC CTCCCTCTCC TCGAAAGCTT CCGGCTCGGC ACCGTGCCGC 
GAGTCCTCGG CTCAGCCCGG GGCGCCCTCG CAGGGGGAGC GGGCCCGATC ACCGCAGCCC 
TGCGGCCCGT CGGAGGAGGG CAGGGCCCCC GGGGAGAGGC AGAGGCCCCA CGCTGGCCGG 
GGACCGCCGT CGGTCATCAG CAGGCGCAGC CCCGGGCTGG CCGCCCAGCC GGACTCCGGC 
CCCAGGACCA GCCAGAGGAG CGTCCAGGTG TCGGTCACCC CGTCGTCCTC CCAGCTGTCC 
TCCTCCGGCT CGTCGTCCTC GTCCGGGGCG CTCGTCCCGC AGGCCTCCCG GTGCTCCCTG 
ACCAAGGCCT GCCGCCAGCC GCCCGTCGTC TTCCTGCCCA AGCTGGTGTA TGACATGGTC 
ACGGCCACCG ACAGCAGCGG CCTGCCCAAG GCCGCCTCGC TCCTGCCCTC CCACTCGGTC 
ATGTGGGCCA GCTCCTTCCG CCCGCTGCTC AGCAAGACCA TGACGTCCAC GGAGCAGTCC 
CTCTACTACC GGCAGTGGAC GGTGCCCCGG CCCAGCCACA TGGACTACAG CAACCGCACC 
GAGGGCCGCG TGGACGGCTT CCACCCGCGC CGGCTGCTGC TCAGCGGGCC CCCTCAGGTT 
GGGAAGACAG GTGCTTACCT GCAGTTCCTT AGTATTTTGT CCAGAATGCT CATCCGGCTG 
ACGGAAGTGG ATGTTTATGA TGAAGAAGAG ATCAATATCC ACCTCAGAGA GGAGTCCGAT 
TGGCATTACC TCCAGCTCAG TGACCCCTGG CCAGACCTGG AGCTATTCAA GAAGTTGCCT 
TTTGACTACA TTATTCACGA CCCGAAGTAT GAAGACGCCA GTCTGATCTG TTCGCACCTT 
CAGAGCGTGA AGAGTGAAGC CAGGGGGACG TCCCGGAAGC CGGAGGAGCT CTACGTGCGG 
CGCCAGACAG CGCGGATGAG GCTGTCCAAG TACGCGGCCT ATAACACATA CCACCACTGC 
GAGCAGTGTC ACCACTACAT GGGCTTCCAC CCCCGCTACC AGCTCTATGA GTCCACCCTG 
CACGCCTTTG CCTTCTCTTA CTCCATGCTA GGAGAGGAAA TCCAGCTACA TTTCATCATC 
CCCAAGTCCA AAGAGCACCA CTTTGTCTTC AGCCAACCCG GAGGCCAGCT GGAGAGCATG 
CGGCTGCCCC TGGTCACAGA CAAGAGCCAC GAGTATATAA AAAGTCCGAC GTTCACCCCG 
ACAACCGGCC GTCACGAACA CGGACTCTTT AACCTGTACC ATGCCATGGA TGGTGCCAGC 
CATCTGCACG TGCTGGTCGT CAAGGAGTAT GAAATGGCCA TTTATAAGAA ATACTGGCCC 
AACCACATCA TGCTGGTGCT CCCAAGTATC TTCAACAGCG CTGGAGTTGG CGCCGCCCAC 
TTCCTGATCA AGGAGCTGTC TTACCACAAT CTGGAGCTGG AGAGGAACCG GCAGGAGGAG 
CTGGGGATCA AGCCACAGGA CATCTGGCCT TTCATCGTGA TCTCGGACGA CTCCTGCGTG 
ATGTGGAACG TGGTGGATGT AGACTGTGGC GGAGAAAGAA GCAGGGAGTT TTCCTGGGCA 
GAAAGAAACG TGTCTTTGAA GCACATCATG CAGCACATCG AGGCGTCCCC CAACATCACC 
CACTATGCCC TGATTGGCAT GCGGAAGTGG TCCAGCAAGA CGGGGAGCGC CGAGGTGCGG 
GAGCCCTTCT CCCGCTGCCA CGTGCACGAC TTCATCATTC TGAACGTGGA CCTGACCCAG 
AACGTGCAGT ACCACCAGAA CCGGTTCACG TGTGACGACG TGGACTTCAA CCTGCGGGTG 
CATAGCGCCG GCCTCCTGCT CTGCCGTTTC AACCGCTTTA GCGTGATGAA GAAGCAGATT 
GCCGTGGGTG GGCACAGGTC CTGCCACATC ACGTCCAAGG TATCCGACAA CCCCGTGGCC 
ATCGTGCCGG CCCAGTACAT CTGCGCCCCG GACAGCAAGC ACACGTTCCT CGCGGCGCCC 
GCGCAGCTCC TGCTGGAGAA GTTCCTCCAG CACCACAGTC ACCGATTCTT CCCGCTGTCC 
CTGAGGAACC GCGGCCACCC TGTGCTCTCG GTCGACTGCT ACCTTAACCT GGGATCTCAG 
ATTTCTGTGT GTTATGTGAG CTCCAGGCCC CACTCCCTGA ACATCAGCTG CTCTGACTTC 
GTGTTTAGCG GACTCCTGCT ATACCTCTGT GATTCTTTTG TGGGAGCTAG CTTTTTGAAA 
AAGTTTCATT TTCTGAAAGG TGCCACCCTG TGCGTGATCT GCCAGGACCG GAACTCCCTG 
CGCCAGACGG TGGTCCGCCT CGAGCTGGAA GACGAGTGGC AGTTCCGGCT GCGTGACGAA 
TTCCAGACGG CCAACGCCAA GGAAGACCGG CCGCTCTTTT TTCTGACCGG GCGGCACATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_027077916.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026933717.1 Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X4, mRNA.

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CloneID	OAc233925
Clone ID Related Accession (Same CDS sequence)	XM_027077916.1 , XM_027077920.1 , XM_027077921.1 , XM_027077917.1 , XM_027077918.1 , XM_027077919.1
Accession Version	XM_027077916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	protein GREB1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834765.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCCGG CCAGCTGAAG ACGACGCGGT TCGAAGAGGT CTTGCACAAC 
TCCATCGAGG CTTCGCTGCG GTCCAACAGC CTGGTGCCCA GGCCCGTCTT CTCCCAGTTG 
TATCTAGAAG CCGAACAGCA GCTCTCCACG CTAGAAGGTG GAGGCCGAGG GGACAATGAG 
GATGAGGAAG AAGAGGGGGA AGGGGCGCTG GAGCCCAGCG GTCCCCCGAA TCCCTACCAG 
ATGCACCCTC CGCCGGAAGG CTGCTGCACC ACAGACGGGT TCTGCCAGGC GGGGAAGGAC 
CTGCGTCTCG TCTCCATTTC CAGTGAGCCC ATAGACGTCC CTGCAGGCTT TCTCCTCGTG 
GGGGCCAAGT CCCCCAGCCT GCCAGACCAT CTCCTGGTGT GTGCTGTGGA CAAAAGGTTC 
TTGCCGGATG ACAATGGGCA CAACGCTCTT CTTGGTTTCT CTGGGAATTG CGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA CTTCACAGAA TTCTCTAATC ACATAAATCT CAAGTTGACC 
ACTCAGCCCA AGAAGCAGAA ACACTTGAAG TATTACCTGG TCCGCAACGC ACAAGGGACT 
CTAACCAAAG GACCTCTCAT CTGTTGGAAA GGCTCAGAGT TCAGAAGCCG GCCGACCTCC 
GCCAGTGCCT GTTCCAGCTC ACTCTTCCCA CCAGCGGAGA GCTCCGGGCC TCTGGCTGCC 
TTCTCCAGCG AGCCCGTTCC CGGAGCCAGC CCGAGCGTCC CCCTGGGAGC CCAGCAGGCA 
GTGCCAGCCT CTGAGCACCC CTCACTTGCT ACGGCCGTGG GTCCAGCTGT TTTCAATGGC 
AGAGATTCTC CGAAGCACCA ACAGCTGGTG AAGAACGACC TATCGGCCAT GCCACGGCCC 
TCGGCATTAG GTATCTTATC AAACTCTGGG CCCCCGAAGA AACGCCACAA AGGGTGGTCC 
CCTGAATCTC CATCAGCGAC AGACGCCGGC TACGCCCAGG GTGGTGGGAA CAGAGCTAAG 
TACGAGAGCG CAGGCATGTC CTGCGTGCCA CAGGTTGGCT CGGTGGGACC AGCGTCGGTC 
ACCTTCCCCG TTGTGGCCTC TGGAGAACCA GTGTCCGTCC CTGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCGGTGAT ATTTAAAGGC CACGGGAACT TCCCTTACCT CTGCGGGAAC 
CTGAGTGACG TCGTCGTGAG CCCCCTCCTG TACACGTGCT ACCAGAACTC TCAGTCCATC 
TCCCGGGCGT ATGAGCACTA CGGGGCCTCG GCGATACAGC CCATCTCCGA GGAGGTGCAG 
CTCCTACTGA CCGTCTACTA TCTCGTCCAG CTGGCAGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCGTGG CGCGAATCGC ACCTGACCGA GATCCGGCAG 
TACCAGCAGG CGCAGCCGCA GCCCTGCCCG CCCGCGCCCA GCGCCGTGGC CCCCGTCACC 
TCGGCCCAGC TGCCCTGGCT CGCCGGCCTG GCCGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC CCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTCATC 
GAGGGCAAGC TCGCCAAGAC CAACTACGTG GTGATCGTCC GCGCGTGCCG CAGCCCCGCC 
ATCGACTCCT GCGTCGCCGT CACGGGAAAA TACCAAGCCC GAATTCTTTC TGAGAGCCTC 
CTCACCCCAG CGGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACCGG GAAAGTGGAC 
TTGTTGGGGG CGTTTTTCAG CACCCTCTGT CCAGAGGGGG ACATTGACAT TTTGCTGGAC 
AAATTTCATC AGGAAAATCA AGGCCATATT TCGTCGTCAC TTACTGCCTC CTCTGTCACC 
AAATCAGCAT CCCTGGATGC ACCGGCATGC ACAAGTTATA ATCTGGAGCC CCACCACATC 
CGGCCTTTCC AGCTGGCCGT GGCTCAGAAG CTCCTCTCCC ATGTGTGTTC AATTGCGGAT 
TCCAGCACCC AGAATTTGGA CTTAGGATCC TTCGAGAAGG TGGACTTTCT GATTTGTATT 
CCACCCTCAG AAGTGACCTA TCAGCAAACC CTGTTCCACG TCTGGCATTC AGGGGTTTTG 
TCAGAGCTCG GCTTGGAAAA GGAGCACATG ACCAAGCAGA GGGTTGAACA GTATGTTTTG 
AAGCTTGATG CTGAAGCGCA GACAAAATTT AAGTCCTTTC TGCAAAACTC CTTTCAGAAT 
CCGCATACGC TCTTTGTCCT GATCCACGAC CACGCCCACT GGGACCTCGT GAGTAGCGCT 
GTTCATAATC TCTATTCCCA AAGTGACCCG GCTGTGGGAT TGGTGGACCG GTTGCTGAAC 
TGCAGAGAGG TGAAGGAGGC CCCGAACATC GTGACGCTTC ACGTGACTTC CTTCCCTTAC 
GCTCTGCAGA CACAGCACAC CCTCATCAGC CCTTACAATG AGATCCACTG GCCCGCCTCC 
TACAGCAATG GGGTGGACTT ATATCCTGAG AACAAGAAGT ACTTCGGGCT GTCAGAGTTC 
ATTGAGTCCA CCCTTTCGGG ACACAGCCTC CCTTTGCTCA GATATGACAG CTCCTTTGAG 
GCCATGGTCA CGGCATTAGG AAAAAGGTTC CCCCGCCTGC ACAGCGCGGT GATCAGGACC 
TTTGTTCTCG TGCAGCACTA CGCGGCCGCC ATGATGGCCG TGAGCGGCCT GTCGCAGATG 
AAGAACTACA CGTCGGTGGA GACGCTGGAG ATCACCCAGA ACCTCATCAA CTCCCCCAAG 
CAGTGCCCCT GCGGCCACGG GCTCATGGTC CTGCTGCGGG TGCCCTGCTC CCCGCTGGCG 
GCGGTGGCCT ACGAGCGGCT GGCCCACGTG CGGGCACGCC TGGCCCTGGA GGAGCACTTT 
GAGATCATCC TGGGCAACCC CAGCTCCGGC ATCACCGTCG GGAAGCACTT CGTGAAGCAA 
CTCAAGCTGT GGCAGAAGAT CGAGGACGCC GAGTGGAGAC CTCAGACCTA CCTGGAGCTG 
GAGGGCCTGC CCTGCATCCT GATCTTCAGT GGGATGGACC CTCACGGGGA ATCCTTACCA 
AGGTCTCTGA GGTACTGTGA CCTGCGTCTG ATAAACTCCT CCTGCTTGGT GAGAACAGCG 
CTGGAGCAGG AGCTGGGCCT GGCCGCTTAC TTTGTGAGCA ACGAGGTTTC TTTGGAGAAG 
GCGGCCCGGA ACGAGCCCTT GGAGAGCGAT GCGGAAAAGC TGAGCAGCAC AGACAACGAG 
GACGAGGAGC TGGTGACGGA AGGCTCCACC TCGGAGAAGA GGAGCCCGGT GAAGCGGGAG 
AGGCCCTGCT CCCATGATTC GGCGGCGTCC TCCCTCTCCT CGAAAGCTTC CGGCTCGGCA 
CCGTGCCGCG AGTCCTCGGC TCAGCCCGGG GCGCCCTCGC AGGGGGAGCG GGCCCGATCA 
CCGCAGCCCT GCGGCCCGTC GGAGGAGGGC AGGGCCCCCG GGGAGAGGCA GAGGCCCCAC 
GCTGGCCGGG GACCGCCGTC GGTCATCAGC AGGCGCAGCC CCGGGCTGGC CGCCCAGCCG 
GACTCCGGCC CCAGGACCAG CCAGAGGAGC GTCCAGGTGT CGGTCACCCC GTCGTCCTCC 
CAGCTGTCCT CCTCCGGCTC GTCGTCCTCG TCCGGGGCGC TCGTCCCGCA GGCCTCCCGG 
TGCTCCCTGA CCAAGGCCTG CCGCCAGCCG CCCGTCGTCT TCCTGCCCAA GCTGGTGTAT 
GACATGGTCA CGGCCACCGA CAGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCC 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGC CCGCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCCC TCTACTACCG GCAGTGGACG GTGCCCCGGC CCAGCCACAT GGACTACAGC 
AACCGCACCG AGGGCCGCGT GGACGGCTTC CACCCGCGCC GGCTGCTGCT CAGCGGGCCC 
CCTCAGGTTG GGAAGACAGG TGCTTACCTG CAGTTCCTTA GTATTTTGTC CAGAATGCTC 
ATCCGGCTGA CGGAAGTGGA TGTTTATGAT GAAGAAGAGA TCAATATCCA CCTCAGAGAG 
GAGTCCGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CAGACCTGGA GCTATTCAAG 
AAGTTGCCTT TTGACTACAT TATTCACGAC CCGAAGTATG AAGACGCCAG TCTGATCTGT 
TCGCACCTTC AGAGCGTGAA GAGTGAAGCC AGGGGGACGT CCCGGAAGCC GGAGGAGCTC 
TACGTGCGGC GCCAGACAGC GCGGATGAGG CTGTCCAAGT ACGCGGCCTA TAACACATAC 
CACCACTGCG AGCAGTGTCA CCACTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTACAT 
TTCATCATCC CCAAGTCCAA AGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GGCTGCCCCT GGTCACAGAC AAGAGCCACG AGTATATAAA AAGTCCGACG 
TTCACCCCGA CAACCGGCCG TCACGAACAC GGACTCTTTA ACCTGTACCA TGCCATGGAT 
GGTGCCAGCC ATCTGCACGT GCTGGTCGTC AAGGAGTATG AAATGGCCAT TTATAAGAAA 
TACTGGCCCA ACCACATCAT GCTGGTGCTC CCAAGTATCT TCAACAGCGC TGGAGTTGGC 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAATC TGGAGCTGGA GAGGAACCGG 
CAGGAGGAGC TGGGGATCAA GCCACAGGAC ATCTGGCCTT TCATCGTGAT CTCGGACGAC 
TCCTGCGTGA TGTGGAACGT GGTGGATGTA GACTGTGGCG GAGAAAGAAG CAGGGAGTTT 
TCCTGGGCAG AAAGAAACGT GTCTTTGAAG CACATCATGC AGCACATCGA GGCGTCCCCC 
AACATCACCC ACTATGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC GGGGAGCGCC 
GAGGTGCGGG AGCCCTTCTC CCGCTGCCAC GTGCACGACT TCATCATTCT GAACGTGGAC 
CTGACCCAGA ACGTGCAGTA CCACCAGAAC CGGTTCACGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ATAGCGCCGG CCTCCTGCTC TGCCGTTTCA ACCGCTTTAG CGTGATGAAG 
AAGCAGATTG CCGTGGGTGG GCACAGGTCC TGCCACATCA CGTCCAAGGT ATCCGACAAC 
CCCGTGGCCA TCGTGCCGGC CCAGTACATC TGCGCCCCGG ACAGCAAGCA CACGTTCCTC 
GCGGCGCCCG CGCAGCTCCT GCTGGAGAAG TTCCTCCAGC ACCACAGTCA CCGATTCTTC 
CCGCTGTCCC TGAGGAACCG CGGCCACCCT GTGCTCTCGG TCGACTGCTA CCTTAACCTG 
GGATCTCAGA TTTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCCCTGAA CATCAGCTGC 
TCTGACTTCG TGTTTAGCGG ACTCCTGCTA TACCTCTGTG ATTCTTTTGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCCTGT GCGTGATCTG CCAGGACCGG 
AACTCCCTGC GCCAGACGGT GGTCCGCCTC GAGCTGGAAG ACGAGTGGCA GTTCCGGCTG 
CGTGACGAAT TCCAGACGGC CAACGCCAAG GAAGACCGGC CGCTCTTTTT TCTGACCGGG 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026933717.1
CDS	533..6364
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPVFSQLYLEAEQQLSTLEGGGRGDNEDEEEEGEGALEPSGPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGTLTKGPLICWKGSEFRSRPTSASACSSSLFPPAESSGPLAAFSSEPVPGASPSVPLGAQQAVPASEHPSLATAVGPAVFNGRDSPKHQQLVKNDLSAMPRPSALGILSNSGPPKKRHKGWSPESPSATDAGYAQGGGNRAKYESAGMSCVPQVGSVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLSDVVVSPLLYTCYQNSQSISRAYEHYGASAIQPISEEVQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAQPQPCPPAPSAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIVRACRSPAIDSCVAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDLLGAFFSTLCPEGDIDILLDKFHQENQGHISSSLTASSVTKSASLDAPACTSYNLEPHHIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLSELGLEKEHMTKQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPAVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPASYSNGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPSSGITVGKHFVKQLKLWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNEVSLEKAARNEPLESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERPCSHDSAASSLSSKASGSAPCRESSAQPGAPSQGERARSPQPCGPSEEGRAPGERQRPHAGRGPPSVISRRSPGLAAQPDSGPRTSQRSVQVSVTPSSSQLSSSGSSSSSGALVPQASRCSLTKACRQPPVVFLPKLVYDMVTATDSSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPSHMDYSNRTEGRVDGFHPRRLLLSGPPQVGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINIHLREESDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHLQSVKSEARGTSRKPEELYVRRQTARMRLSKYAAYNTYHHCEQCHHYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDCGGERSREFSWAERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSAEVREPFSRCHVHDFIILNVDLTQNVQYHQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSCHITSKVSDNPVAIVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLRNRGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_027077916.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027077916.1

ORF Insert Sequence:

ATGGGAAACT CGTACGCCGG CCAGCTGAAG ACGACGCGGT TCGAAGAGGT CTTGCACAAC 
TCCATCGAGG CTTCGCTGCG GTCCAACAGC CTGGTGCCCA GGCCCGTCTT CTCCCAGTTG 
TATCTAGAAG CCGAACAGCA GCTCTCCACG CTAGAAGGTG GAGGCCGAGG GGACAATGAG 
GATGAGGAAG AAGAGGGGGA AGGGGCGCTG GAGCCCAGCG GTCCCCCGAA TCCCTACCAG 
ATGCACCCTC CGCCGGAAGG CTGCTGCACC ACAGACGGGT TCTGCCAGGC GGGGAAGGAC 
CTGCGTCTCG TCTCCATTTC CAGTGAGCCC ATAGACGTCC CTGCAGGCTT TCTCCTCGTG 
GGGGCCAAGT CCCCCAGCCT GCCAGACCAT CTCCTGGTGT GTGCTGTGGA CAAAAGGTTC 
TTGCCGGATG ACAATGGGCA CAACGCTCTT CTTGGTTTCT CTGGGAATTG CGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA CTTCACAGAA TTCTCTAATC ACATAAATCT CAAGTTGACC 
ACTCAGCCCA AGAAGCAGAA ACACTTGAAG TATTACCTGG TCCGCAACGC ACAAGGGACT 
CTAACCAAAG GACCTCTCAT CTGTTGGAAA GGCTCAGAGT TCAGAAGCCG GCCGACCTCC 
GCCAGTGCCT GTTCCAGCTC ACTCTTCCCA CCAGCGGAGA GCTCCGGGCC TCTGGCTGCC 
TTCTCCAGCG AGCCCGTTCC CGGAGCCAGC CCGAGCGTCC CCCTGGGAGC CCAGCAGGCA 
GTGCCAGCCT CTGAGCACCC CTCACTTGCT ACGGCCGTGG GTCCAGCTGT TTTCAATGGC 
AGAGATTCTC CGAAGCACCA ACAGCTGGTG AAGAACGACC TATCGGCCAT GCCACGGCCC 
TCGGCATTAG GTATCTTATC AAACTCTGGG CCCCCGAAGA AACGCCACAA AGGGTGGTCC 
CCTGAATCTC CATCAGCGAC AGACGCCGGC TACGCCCAGG GTGGTGGGAA CAGAGCTAAG 
TACGAGAGCG CAGGCATGTC CTGCGTGCCA CAGGTTGGCT CGGTGGGACC AGCGTCGGTC 
ACCTTCCCCG TTGTGGCCTC TGGAGAACCA GTGTCCGTCC CTGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCGGTGAT ATTTAAAGGC CACGGGAACT TCCCTTACCT CTGCGGGAAC 
CTGAGTGACG TCGTCGTGAG CCCCCTCCTG TACACGTGCT ACCAGAACTC TCAGTCCATC 
TCCCGGGCGT ATGAGCACTA CGGGGCCTCG GCGATACAGC CCATCTCCGA GGAGGTGCAG 
CTCCTACTGA CCGTCTACTA TCTCGTCCAG CTGGCAGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCGTGG CGCGAATCGC ACCTGACCGA GATCCGGCAG 
TACCAGCAGG CGCAGCCGCA GCCCTGCCCG CCCGCGCCCA GCGCCGTGGC CCCCGTCACC 
TCGGCCCAGC TGCCCTGGCT CGCCGGCCTG GCCGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC CCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTCATC 
GAGGGCAAGC TCGCCAAGAC CAACTACGTG GTGATCGTCC GCGCGTGCCG CAGCCCCGCC 
ATCGACTCCT GCGTCGCCGT CACGGGAAAA TACCAAGCCC GAATTCTTTC TGAGAGCCTC 
CTCACCCCAG CGGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACCGG GAAAGTGGAC 
TTGTTGGGGG CGTTTTTCAG CACCCTCTGT CCAGAGGGGG ACATTGACAT TTTGCTGGAC 
AAATTTCATC AGGAAAATCA AGGCCATATT TCGTCGTCAC TTACTGCCTC CTCTGTCACC 
AAATCAGCAT CCCTGGATGC ACCGGCATGC ACAAGTTATA ATCTGGAGCC CCACCACATC 
CGGCCTTTCC AGCTGGCCGT GGCTCAGAAG CTCCTCTCCC ATGTGTGTTC AATTGCGGAT 
TCCAGCACCC AGAATTTGGA CTTAGGATCC TTCGAGAAGG TGGACTTTCT GATTTGTATT 
CCACCCTCAG AAGTGACCTA TCAGCAAACC CTGTTCCACG TCTGGCATTC AGGGGTTTTG 
TCAGAGCTCG GCTTGGAAAA GGAGCACATG ACCAAGCAGA GGGTTGAACA GTATGTTTTG 
AAGCTTGATG CTGAAGCGCA GACAAAATTT AAGTCCTTTC TGCAAAACTC CTTTCAGAAT 
CCGCATACGC TCTTTGTCCT GATCCACGAC CACGCCCACT GGGACCTCGT GAGTAGCGCT 
GTTCATAATC TCTATTCCCA AAGTGACCCG GCTGTGGGAT TGGTGGACCG GTTGCTGAAC 
TGCAGAGAGG TGAAGGAGGC CCCGAACATC GTGACGCTTC ACGTGACTTC CTTCCCTTAC 
GCTCTGCAGA CACAGCACAC CCTCATCAGC CCTTACAATG AGATCCACTG GCCCGCCTCC 
TACAGCAATG GGGTGGACTT ATATCCTGAG AACAAGAAGT ACTTCGGGCT GTCAGAGTTC 
ATTGAGTCCA CCCTTTCGGG ACACAGCCTC CCTTTGCTCA GATATGACAG CTCCTTTGAG 
GCCATGGTCA CGGCATTAGG AAAAAGGTTC CCCCGCCTGC ACAGCGCGGT GATCAGGACC 
TTTGTTCTCG TGCAGCACTA CGCGGCCGCC ATGATGGCCG TGAGCGGCCT GTCGCAGATG 
AAGAACTACA CGTCGGTGGA GACGCTGGAG ATCACCCAGA ACCTCATCAA CTCCCCCAAG 
CAGTGCCCCT GCGGCCACGG GCTCATGGTC CTGCTGCGGG TGCCCTGCTC CCCGCTGGCG 
GCGGTGGCCT ACGAGCGGCT GGCCCACGTG CGGGCACGCC TGGCCCTGGA GGAGCACTTT 
GAGATCATCC TGGGCAACCC CAGCTCCGGC ATCACCGTCG GGAAGCACTT CGTGAAGCAA 
CTCAAGCTGT GGCAGAAGAT CGAGGACGCC GAGTGGAGAC CTCAGACCTA CCTGGAGCTG 
GAGGGCCTGC CCTGCATCCT GATCTTCAGT GGGATGGACC CTCACGGGGA ATCCTTACCA 
AGGTCTCTGA GGTACTGTGA CCTGCGTCTG ATAAACTCCT CCTGCTTGGT GAGAACAGCG 
CTGGAGCAGG AGCTGGGCCT GGCCGCTTAC TTTGTGAGCA ACGAGGTTTC TTTGGAGAAG 
GCGGCCCGGA ACGAGCCCTT GGAGAGCGAT GCGGAAAAGC TGAGCAGCAC AGACAACGAG 
GACGAGGAGC TGGTGACGGA AGGCTCCACC TCGGAGAAGA GGAGCCCGGT GAAGCGGGAG 
AGGCCCTGCT CCCATGATTC GGCGGCGTCC TCCCTCTCCT CGAAAGCTTC CGGCTCGGCA 
CCGTGCCGCG AGTCCTCGGC TCAGCCCGGG GCGCCCTCGC AGGGGGAGCG GGCCCGATCA 
CCGCAGCCCT GCGGCCCGTC GGAGGAGGGC AGGGCCCCCG GGGAGAGGCA GAGGCCCCAC 
GCTGGCCGGG GACCGCCGTC GGTCATCAGC AGGCGCAGCC CCGGGCTGGC CGCCCAGCCG 
GACTCCGGCC CCAGGACCAG CCAGAGGAGC GTCCAGGTGT CGGTCACCCC GTCGTCCTCC 
CAGCTGTCCT CCTCCGGCTC GTCGTCCTCG TCCGGGGCGC TCGTCCCGCA GGCCTCCCGG 
TGCTCCCTGA CCAAGGCCTG CCGCCAGCCG CCCGTCGTCT TCCTGCCCAA GCTGGTGTAT 
GACATGGTCA CGGCCACCGA CAGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCC 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGC CCGCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCCC TCTACTACCG GCAGTGGACG GTGCCCCGGC CCAGCCACAT GGACTACAGC 
AACCGCACCG AGGGCCGCGT GGACGGCTTC CACCCGCGCC GGCTGCTGCT CAGCGGGCCC 
CCTCAGGTTG GGAAGACAGG TGCTTACCTG CAGTTCCTTA GTATTTTGTC CAGAATGCTC 
ATCCGGCTGA CGGAAGTGGA TGTTTATGAT GAAGAAGAGA TCAATATCCA CCTCAGAGAG 
GAGTCCGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CAGACCTGGA GCTATTCAAG 
AAGTTGCCTT TTGACTACAT TATTCACGAC CCGAAGTATG AAGACGCCAG TCTGATCTGT 
TCGCACCTTC AGAGCGTGAA GAGTGAAGCC AGGGGGACGT CCCGGAAGCC GGAGGAGCTC 
TACGTGCGGC GCCAGACAGC GCGGATGAGG CTGTCCAAGT ACGCGGCCTA TAACACATAC 
CACCACTGCG AGCAGTGTCA CCACTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTACAT 
TTCATCATCC CCAAGTCCAA AGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GGCTGCCCCT GGTCACAGAC AAGAGCCACG AGTATATAAA AAGTCCGACG 
TTCACCCCGA CAACCGGCCG TCACGAACAC GGACTCTTTA ACCTGTACCA TGCCATGGAT 
GGTGCCAGCC ATCTGCACGT GCTGGTCGTC AAGGAGTATG AAATGGCCAT TTATAAGAAA 
TACTGGCCCA ACCACATCAT GCTGGTGCTC CCAAGTATCT TCAACAGCGC TGGAGTTGGC 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAATC TGGAGCTGGA GAGGAACCGG 
CAGGAGGAGC TGGGGATCAA GCCACAGGAC ATCTGGCCTT TCATCGTGAT CTCGGACGAC 
TCCTGCGTGA TGTGGAACGT GGTGGATGTA GACTGTGGCG GAGAAAGAAG CAGGGAGTTT 
TCCTGGGCAG AAAGAAACGT GTCTTTGAAG CACATCATGC AGCACATCGA GGCGTCCCCC 
AACATCACCC ACTATGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC GGGGAGCGCC 
GAGGTGCGGG AGCCCTTCTC CCGCTGCCAC GTGCACGACT TCATCATTCT GAACGTGGAC 
CTGACCCAGA ACGTGCAGTA CCACCAGAAC CGGTTCACGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ATAGCGCCGG CCTCCTGCTC TGCCGTTTCA ACCGCTTTAG CGTGATGAAG 
AAGCAGATTG CCGTGGGTGG GCACAGGTCC TGCCACATCA CGTCCAAGGT ATCCGACAAC 
CCCGTGGCCA TCGTGCCGGC CCAGTACATC TGCGCCCCGG ACAGCAAGCA CACGTTCCTC 
GCGGCGCCCG CGCAGCTCCT GCTGGAGAAG TTCCTCCAGC ACCACAGTCA CCGATTCTTC 
CCGCTGTCCC TGAGGAACCG CGGCCACCCT GTGCTCTCGG TCGACTGCTA CCTTAACCTG 
GGATCTCAGA TTTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCCCTGAA CATCAGCTGC 
TCTGACTTCG TGTTTAGCGG ACTCCTGCTA TACCTCTGTG ATTCTTTTGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCCTGT GCGTGATCTG CCAGGACCGG 
AACTCCCTGC GCCAGACGGT GGTCCGCCTC GAGCTGGAAG ACGAGTGGCA GTTCCGGCTG 
CGTGACGAAT TCCAGACGGC CAACGCCAAG GAAGACCGGC CGCTCTTTTT TCTGACCGGG 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_027077920.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026933721.1 Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X8, mRNA.

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CloneID	OAc233925
Clone ID Related Accession (Same CDS sequence)	XM_027077916.1 , XM_027077920.1 , XM_027077921.1 , XM_027077917.1 , XM_027077918.1 , XM_027077919.1
Accession Version	XM_027077920.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	protein GREB1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834765.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCCGG CCAGCTGAAG ACGACGCGGT TCGAAGAGGT CTTGCACAAC 
TCCATCGAGG CTTCGCTGCG GTCCAACAGC CTGGTGCCCA GGCCCGTCTT CTCCCAGTTG 
TATCTAGAAG CCGAACAGCA GCTCTCCACG CTAGAAGGTG GAGGCCGAGG GGACAATGAG 
GATGAGGAAG AAGAGGGGGA AGGGGCGCTG GAGCCCAGCG GTCCCCCGAA TCCCTACCAG 
ATGCACCCTC CGCCGGAAGG CTGCTGCACC ACAGACGGGT TCTGCCAGGC GGGGAAGGAC 
CTGCGTCTCG TCTCCATTTC CAGTGAGCCC ATAGACGTCC CTGCAGGCTT TCTCCTCGTG 
GGGGCCAAGT CCCCCAGCCT GCCAGACCAT CTCCTGGTGT GTGCTGTGGA CAAAAGGTTC 
TTGCCGGATG ACAATGGGCA CAACGCTCTT CTTGGTTTCT CTGGGAATTG CGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA CTTCACAGAA TTCTCTAATC ACATAAATCT CAAGTTGACC 
ACTCAGCCCA AGAAGCAGAA ACACTTGAAG TATTACCTGG TCCGCAACGC ACAAGGGACT 
CTAACCAAAG GACCTCTCAT CTGTTGGAAA GGCTCAGAGT TCAGAAGCCG GCCGACCTCC 
GCCAGTGCCT GTTCCAGCTC ACTCTTCCCA CCAGCGGAGA GCTCCGGGCC TCTGGCTGCC 
TTCTCCAGCG AGCCCGTTCC CGGAGCCAGC CCGAGCGTCC CCCTGGGAGC CCAGCAGGCA 
GTGCCAGCCT CTGAGCACCC CTCACTTGCT ACGGCCGTGG GTCCAGCTGT TTTCAATGGC 
AGAGATTCTC CGAAGCACCA ACAGCTGGTG AAGAACGACC TATCGGCCAT GCCACGGCCC 
TCGGCATTAG GTATCTTATC AAACTCTGGG CCCCCGAAGA AACGCCACAA AGGGTGGTCC 
CCTGAATCTC CATCAGCGAC AGACGCCGGC TACGCCCAGG GTGGTGGGAA CAGAGCTAAG 
TACGAGAGCG CAGGCATGTC CTGCGTGCCA CAGGTTGGCT CGGTGGGACC AGCGTCGGTC 
ACCTTCCCCG TTGTGGCCTC TGGAGAACCA GTGTCCGTCC CTGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCGGTGAT ATTTAAAGGC CACGGGAACT TCCCTTACCT CTGCGGGAAC 
CTGAGTGACG TCGTCGTGAG CCCCCTCCTG TACACGTGCT ACCAGAACTC TCAGTCCATC 
TCCCGGGCGT ATGAGCACTA CGGGGCCTCG GCGATACAGC CCATCTCCGA GGAGGTGCAG 
CTCCTACTGA CCGTCTACTA TCTCGTCCAG CTGGCAGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCGTGG CGCGAATCGC ACCTGACCGA GATCCGGCAG 
TACCAGCAGG CGCAGCCGCA GCCCTGCCCG CCCGCGCCCA GCGCCGTGGC CCCCGTCACC 
TCGGCCCAGC TGCCCTGGCT CGCCGGCCTG GCCGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC CCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTCATC 
GAGGGCAAGC TCGCCAAGAC CAACTACGTG GTGATCGTCC GCGCGTGCCG CAGCCCCGCC 
ATCGACTCCT GCGTCGCCGT CACGGGAAAA TACCAAGCCC GAATTCTTTC TGAGAGCCTC 
CTCACCCCAG CGGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACCGG GAAAGTGGAC 
TTGTTGGGGG CGTTTTTCAG CACCCTCTGT CCAGAGGGGG ACATTGACAT TTTGCTGGAC 
AAATTTCATC AGGAAAATCA AGGCCATATT TCGTCGTCAC TTACTGCCTC CTCTGTCACC 
AAATCAGCAT CCCTGGATGC ACCGGCATGC ACAAGTTATA ATCTGGAGCC CCACCACATC 
CGGCCTTTCC AGCTGGCCGT GGCTCAGAAG CTCCTCTCCC ATGTGTGTTC AATTGCGGAT 
TCCAGCACCC AGAATTTGGA CTTAGGATCC TTCGAGAAGG TGGACTTTCT GATTTGTATT 
CCACCCTCAG AAGTGACCTA TCAGCAAACC CTGTTCCACG TCTGGCATTC AGGGGTTTTG 
TCAGAGCTCG GCTTGGAAAA GGAGCACATG ACCAAGCAGA GGGTTGAACA GTATGTTTTG 
AAGCTTGATG CTGAAGCGCA GACAAAATTT AAGTCCTTTC TGCAAAACTC CTTTCAGAAT 
CCGCATACGC TCTTTGTCCT GATCCACGAC CACGCCCACT GGGACCTCGT GAGTAGCGCT 
GTTCATAATC TCTATTCCCA AAGTGACCCG GCTGTGGGAT TGGTGGACCG GTTGCTGAAC 
TGCAGAGAGG TGAAGGAGGC CCCGAACATC GTGACGCTTC ACGTGACTTC CTTCCCTTAC 
GCTCTGCAGA CACAGCACAC CCTCATCAGC CCTTACAATG AGATCCACTG GCCCGCCTCC 
TACAGCAATG GGGTGGACTT ATATCCTGAG AACAAGAAGT ACTTCGGGCT GTCAGAGTTC 
ATTGAGTCCA CCCTTTCGGG ACACAGCCTC CCTTTGCTCA GATATGACAG CTCCTTTGAG 
GCCATGGTCA CGGCATTAGG AAAAAGGTTC CCCCGCCTGC ACAGCGCGGT GATCAGGACC 
TTTGTTCTCG TGCAGCACTA CGCGGCCGCC ATGATGGCCG TGAGCGGCCT GTCGCAGATG 
AAGAACTACA CGTCGGTGGA GACGCTGGAG ATCACCCAGA ACCTCATCAA CTCCCCCAAG 
CAGTGCCCCT GCGGCCACGG GCTCATGGTC CTGCTGCGGG TGCCCTGCTC CCCGCTGGCG 
GCGGTGGCCT ACGAGCGGCT GGCCCACGTG CGGGCACGCC TGGCCCTGGA GGAGCACTTT 
GAGATCATCC TGGGCAACCC CAGCTCCGGC ATCACCGTCG GGAAGCACTT CGTGAAGCAA 
CTCAAGCTGT GGCAGAAGAT CGAGGACGCC GAGTGGAGAC CTCAGACCTA CCTGGAGCTG 
GAGGGCCTGC CCTGCATCCT GATCTTCAGT GGGATGGACC CTCACGGGGA ATCCTTACCA 
AGGTCTCTGA GGTACTGTGA CCTGCGTCTG ATAAACTCCT CCTGCTTGGT GAGAACAGCG 
CTGGAGCAGG AGCTGGGCCT GGCCGCTTAC TTTGTGAGCA ACGAGGTTTC TTTGGAGAAG 
GCGGCCCGGA ACGAGCCCTT GGAGAGCGAT GCGGAAAAGC TGAGCAGCAC AGACAACGAG 
GACGAGGAGC TGGTGACGGA AGGCTCCACC TCGGAGAAGA GGAGCCCGGT GAAGCGGGAG 
AGGCCCTGCT CCCATGATTC GGCGGCGTCC TCCCTCTCCT CGAAAGCTTC CGGCTCGGCA 
CCGTGCCGCG AGTCCTCGGC TCAGCCCGGG GCGCCCTCGC AGGGGGAGCG GGCCCGATCA 
CCGCAGCCCT GCGGCCCGTC GGAGGAGGGC AGGGCCCCCG GGGAGAGGCA GAGGCCCCAC 
GCTGGCCGGG GACCGCCGTC GGTCATCAGC AGGCGCAGCC CCGGGCTGGC CGCCCAGCCG 
GACTCCGGCC CCAGGACCAG CCAGAGGAGC GTCCAGGTGT CGGTCACCCC GTCGTCCTCC 
CAGCTGTCCT CCTCCGGCTC GTCGTCCTCG TCCGGGGCGC TCGTCCCGCA GGCCTCCCGG 
TGCTCCCTGA CCAAGGCCTG CCGCCAGCCG CCCGTCGTCT TCCTGCCCAA GCTGGTGTAT 
GACATGGTCA CGGCCACCGA CAGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCC 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGC CCGCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCCC TCTACTACCG GCAGTGGACG GTGCCCCGGC CCAGCCACAT GGACTACAGC 
AACCGCACCG AGGGCCGCGT GGACGGCTTC CACCCGCGCC GGCTGCTGCT CAGCGGGCCC 
CCTCAGGTTG GGAAGACAGG TGCTTACCTG CAGTTCCTTA GTATTTTGTC CAGAATGCTC 
ATCCGGCTGA CGGAAGTGGA TGTTTATGAT GAAGAAGAGA TCAATATCCA CCTCAGAGAG 
GAGTCCGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CAGACCTGGA GCTATTCAAG 
AAGTTGCCTT TTGACTACAT TATTCACGAC CCGAAGTATG AAGACGCCAG TCTGATCTGT 
TCGCACCTTC AGAGCGTGAA GAGTGAAGCC AGGGGGACGT CCCGGAAGCC GGAGGAGCTC 
TACGTGCGGC GCCAGACAGC GCGGATGAGG CTGTCCAAGT ACGCGGCCTA TAACACATAC 
CACCACTGCG AGCAGTGTCA CCACTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTACAT 
TTCATCATCC CCAAGTCCAA AGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GGCTGCCCCT GGTCACAGAC AAGAGCCACG AGTATATAAA AAGTCCGACG 
TTCACCCCGA CAACCGGCCG TCACGAACAC GGACTCTTTA ACCTGTACCA TGCCATGGAT 
GGTGCCAGCC ATCTGCACGT GCTGGTCGTC AAGGAGTATG AAATGGCCAT TTATAAGAAA 
TACTGGCCCA ACCACATCAT GCTGGTGCTC CCAAGTATCT TCAACAGCGC TGGAGTTGGC 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAATC TGGAGCTGGA GAGGAACCGG 
CAGGAGGAGC TGGGGATCAA GCCACAGGAC ATCTGGCCTT TCATCGTGAT CTCGGACGAC 
TCCTGCGTGA TGTGGAACGT GGTGGATGTA GACTGTGGCG GAGAAAGAAG CAGGGAGTTT 
TCCTGGGCAG AAAGAAACGT GTCTTTGAAG CACATCATGC AGCACATCGA GGCGTCCCCC 
AACATCACCC ACTATGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC GGGGAGCGCC 
GAGGTGCGGG AGCCCTTCTC CCGCTGCCAC GTGCACGACT TCATCATTCT GAACGTGGAC 
CTGACCCAGA ACGTGCAGTA CCACCAGAAC CGGTTCACGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ATAGCGCCGG CCTCCTGCTC TGCCGTTTCA ACCGCTTTAG CGTGATGAAG 
AAGCAGATTG CCGTGGGTGG GCACAGGTCC TGCCACATCA CGTCCAAGGT ATCCGACAAC 
CCCGTGGCCA TCGTGCCGGC CCAGTACATC TGCGCCCCGG ACAGCAAGCA CACGTTCCTC 
GCGGCGCCCG CGCAGCTCCT GCTGGAGAAG TTCCTCCAGC ACCACAGTCA CCGATTCTTC 
CCGCTGTCCC TGAGGAACCG CGGCCACCCT GTGCTCTCGG TCGACTGCTA CCTTAACCTG 
GGATCTCAGA TTTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCCCTGAA CATCAGCTGC 
TCTGACTTCG TGTTTAGCGG ACTCCTGCTA TACCTCTGTG ATTCTTTTGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCCTGT GCGTGATCTG CCAGGACCGG 
AACTCCCTGC GCCAGACGGT GGTCCGCCTC GAGCTGGAAG ACGAGTGGCA GTTCCGGCTG 
CGTGACGAAT TCCAGACGGC CAACGCCAAG GAAGACCGGC CGCTCTTTTT TCTGACCGGG 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026933721.1
CDS	829..6660
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPVFSQLYLEAEQQLSTLEGGGRGDNEDEEEEGEGALEPSGPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGTLTKGPLICWKGSEFRSRPTSASACSSSLFPPAESSGPLAAFSSEPVPGASPSVPLGAQQAVPASEHPSLATAVGPAVFNGRDSPKHQQLVKNDLSAMPRPSALGILSNSGPPKKRHKGWSPESPSATDAGYAQGGGNRAKYESAGMSCVPQVGSVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLSDVVVSPLLYTCYQNSQSISRAYEHYGASAIQPISEEVQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAQPQPCPPAPSAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIVRACRSPAIDSCVAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDLLGAFFSTLCPEGDIDILLDKFHQENQGHISSSLTASSVTKSASLDAPACTSYNLEPHHIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLSELGLEKEHMTKQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPAVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPASYSNGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPSSGITVGKHFVKQLKLWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNEVSLEKAARNEPLESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERPCSHDSAASSLSSKASGSAPCRESSAQPGAPSQGERARSPQPCGPSEEGRAPGERQRPHAGRGPPSVISRRSPGLAAQPDSGPRTSQRSVQVSVTPSSSQLSSSGSSSSSGALVPQASRCSLTKACRQPPVVFLPKLVYDMVTATDSSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPSHMDYSNRTEGRVDGFHPRRLLLSGPPQVGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINIHLREESDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHLQSVKSEARGTSRKPEELYVRRQTARMRLSKYAAYNTYHHCEQCHHYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDCGGERSREFSWAERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSAEVREPFSRCHVHDFIILNVDLTQNVQYHQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSCHITSKVSDNPVAIVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLRNRGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_027077920.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027077920.1

ORF Insert Sequence:

ATGGGAAACT CGTACGCCGG CCAGCTGAAG ACGACGCGGT TCGAAGAGGT CTTGCACAAC 
TCCATCGAGG CTTCGCTGCG GTCCAACAGC CTGGTGCCCA GGCCCGTCTT CTCCCAGTTG 
TATCTAGAAG CCGAACAGCA GCTCTCCACG CTAGAAGGTG GAGGCCGAGG GGACAATGAG 
GATGAGGAAG AAGAGGGGGA AGGGGCGCTG GAGCCCAGCG GTCCCCCGAA TCCCTACCAG 
ATGCACCCTC CGCCGGAAGG CTGCTGCACC ACAGACGGGT TCTGCCAGGC GGGGAAGGAC 
CTGCGTCTCG TCTCCATTTC CAGTGAGCCC ATAGACGTCC CTGCAGGCTT TCTCCTCGTG 
GGGGCCAAGT CCCCCAGCCT GCCAGACCAT CTCCTGGTGT GTGCTGTGGA CAAAAGGTTC 
TTGCCGGATG ACAATGGGCA CAACGCTCTT CTTGGTTTCT CTGGGAATTG CGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA CTTCACAGAA TTCTCTAATC ACATAAATCT CAAGTTGACC 
ACTCAGCCCA AGAAGCAGAA ACACTTGAAG TATTACCTGG TCCGCAACGC ACAAGGGACT 
CTAACCAAAG GACCTCTCAT CTGTTGGAAA GGCTCAGAGT TCAGAAGCCG GCCGACCTCC 
GCCAGTGCCT GTTCCAGCTC ACTCTTCCCA CCAGCGGAGA GCTCCGGGCC TCTGGCTGCC 
TTCTCCAGCG AGCCCGTTCC CGGAGCCAGC CCGAGCGTCC CCCTGGGAGC CCAGCAGGCA 
GTGCCAGCCT CTGAGCACCC CTCACTTGCT ACGGCCGTGG GTCCAGCTGT TTTCAATGGC 
AGAGATTCTC CGAAGCACCA ACAGCTGGTG AAGAACGACC TATCGGCCAT GCCACGGCCC 
TCGGCATTAG GTATCTTATC AAACTCTGGG CCCCCGAAGA AACGCCACAA AGGGTGGTCC 
CCTGAATCTC CATCAGCGAC AGACGCCGGC TACGCCCAGG GTGGTGGGAA CAGAGCTAAG 
TACGAGAGCG CAGGCATGTC CTGCGTGCCA CAGGTTGGCT CGGTGGGACC AGCGTCGGTC 
ACCTTCCCCG TTGTGGCCTC TGGAGAACCA GTGTCCGTCC CTGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCGGTGAT ATTTAAAGGC CACGGGAACT TCCCTTACCT CTGCGGGAAC 
CTGAGTGACG TCGTCGTGAG CCCCCTCCTG TACACGTGCT ACCAGAACTC TCAGTCCATC 
TCCCGGGCGT ATGAGCACTA CGGGGCCTCG GCGATACAGC CCATCTCCGA GGAGGTGCAG 
CTCCTACTGA CCGTCTACTA TCTCGTCCAG CTGGCAGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCGTGG CGCGAATCGC ACCTGACCGA GATCCGGCAG 
TACCAGCAGG CGCAGCCGCA GCCCTGCCCG CCCGCGCCCA GCGCCGTGGC CCCCGTCACC 
TCGGCCCAGC TGCCCTGGCT CGCCGGCCTG GCCGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC CCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTCATC 
GAGGGCAAGC TCGCCAAGAC CAACTACGTG GTGATCGTCC GCGCGTGCCG CAGCCCCGCC 
ATCGACTCCT GCGTCGCCGT CACGGGAAAA TACCAAGCCC GAATTCTTTC TGAGAGCCTC 
CTCACCCCAG CGGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACCGG GAAAGTGGAC 
TTGTTGGGGG CGTTTTTCAG CACCCTCTGT CCAGAGGGGG ACATTGACAT TTTGCTGGAC 
AAATTTCATC AGGAAAATCA AGGCCATATT TCGTCGTCAC TTACTGCCTC CTCTGTCACC 
AAATCAGCAT CCCTGGATGC ACCGGCATGC ACAAGTTATA ATCTGGAGCC CCACCACATC 
CGGCCTTTCC AGCTGGCCGT GGCTCAGAAG CTCCTCTCCC ATGTGTGTTC AATTGCGGAT 
TCCAGCACCC AGAATTTGGA CTTAGGATCC TTCGAGAAGG TGGACTTTCT GATTTGTATT 
CCACCCTCAG AAGTGACCTA TCAGCAAACC CTGTTCCACG TCTGGCATTC AGGGGTTTTG 
TCAGAGCTCG GCTTGGAAAA GGAGCACATG ACCAAGCAGA GGGTTGAACA GTATGTTTTG 
AAGCTTGATG CTGAAGCGCA GACAAAATTT AAGTCCTTTC TGCAAAACTC CTTTCAGAAT 
CCGCATACGC TCTTTGTCCT GATCCACGAC CACGCCCACT GGGACCTCGT GAGTAGCGCT 
GTTCATAATC TCTATTCCCA AAGTGACCCG GCTGTGGGAT TGGTGGACCG GTTGCTGAAC 
TGCAGAGAGG TGAAGGAGGC CCCGAACATC GTGACGCTTC ACGTGACTTC CTTCCCTTAC 
GCTCTGCAGA CACAGCACAC CCTCATCAGC CCTTACAATG AGATCCACTG GCCCGCCTCC 
TACAGCAATG GGGTGGACTT ATATCCTGAG AACAAGAAGT ACTTCGGGCT GTCAGAGTTC 
ATTGAGTCCA CCCTTTCGGG ACACAGCCTC CCTTTGCTCA GATATGACAG CTCCTTTGAG 
GCCATGGTCA CGGCATTAGG AAAAAGGTTC CCCCGCCTGC ACAGCGCGGT GATCAGGACC 
TTTGTTCTCG TGCAGCACTA CGCGGCCGCC ATGATGGCCG TGAGCGGCCT GTCGCAGATG 
AAGAACTACA CGTCGGTGGA GACGCTGGAG ATCACCCAGA ACCTCATCAA CTCCCCCAAG 
CAGTGCCCCT GCGGCCACGG GCTCATGGTC CTGCTGCGGG TGCCCTGCTC CCCGCTGGCG 
GCGGTGGCCT ACGAGCGGCT GGCCCACGTG CGGGCACGCC TGGCCCTGGA GGAGCACTTT 
GAGATCATCC TGGGCAACCC CAGCTCCGGC ATCACCGTCG GGAAGCACTT CGTGAAGCAA 
CTCAAGCTGT GGCAGAAGAT CGAGGACGCC GAGTGGAGAC CTCAGACCTA CCTGGAGCTG 
GAGGGCCTGC CCTGCATCCT GATCTTCAGT GGGATGGACC CTCACGGGGA ATCCTTACCA 
AGGTCTCTGA GGTACTGTGA CCTGCGTCTG ATAAACTCCT CCTGCTTGGT GAGAACAGCG 
CTGGAGCAGG AGCTGGGCCT GGCCGCTTAC TTTGTGAGCA ACGAGGTTTC TTTGGAGAAG 
GCGGCCCGGA ACGAGCCCTT GGAGAGCGAT GCGGAAAAGC TGAGCAGCAC AGACAACGAG 
GACGAGGAGC TGGTGACGGA AGGCTCCACC TCGGAGAAGA GGAGCCCGGT GAAGCGGGAG 
AGGCCCTGCT CCCATGATTC GGCGGCGTCC TCCCTCTCCT CGAAAGCTTC CGGCTCGGCA 
CCGTGCCGCG AGTCCTCGGC TCAGCCCGGG GCGCCCTCGC AGGGGGAGCG GGCCCGATCA 
CCGCAGCCCT GCGGCCCGTC GGAGGAGGGC AGGGCCCCCG GGGAGAGGCA GAGGCCCCAC 
GCTGGCCGGG GACCGCCGTC GGTCATCAGC AGGCGCAGCC CCGGGCTGGC CGCCCAGCCG 
GACTCCGGCC CCAGGACCAG CCAGAGGAGC GTCCAGGTGT CGGTCACCCC GTCGTCCTCC 
CAGCTGTCCT CCTCCGGCTC GTCGTCCTCG TCCGGGGCGC TCGTCCCGCA GGCCTCCCGG 
TGCTCCCTGA CCAAGGCCTG CCGCCAGCCG CCCGTCGTCT TCCTGCCCAA GCTGGTGTAT 
GACATGGTCA CGGCCACCGA CAGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCC 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGC CCGCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCCC TCTACTACCG GCAGTGGACG GTGCCCCGGC CCAGCCACAT GGACTACAGC 
AACCGCACCG AGGGCCGCGT GGACGGCTTC CACCCGCGCC GGCTGCTGCT CAGCGGGCCC 
CCTCAGGTTG GGAAGACAGG TGCTTACCTG CAGTTCCTTA GTATTTTGTC CAGAATGCTC 
ATCCGGCTGA CGGAAGTGGA TGTTTATGAT GAAGAAGAGA TCAATATCCA CCTCAGAGAG 
GAGTCCGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CAGACCTGGA GCTATTCAAG 
AAGTTGCCTT TTGACTACAT TATTCACGAC CCGAAGTATG AAGACGCCAG TCTGATCTGT 
TCGCACCTTC AGAGCGTGAA GAGTGAAGCC AGGGGGACGT CCCGGAAGCC GGAGGAGCTC 
TACGTGCGGC GCCAGACAGC GCGGATGAGG CTGTCCAAGT ACGCGGCCTA TAACACATAC 
CACCACTGCG AGCAGTGTCA CCACTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTACAT 
TTCATCATCC CCAAGTCCAA AGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GGCTGCCCCT GGTCACAGAC AAGAGCCACG AGTATATAAA AAGTCCGACG 
TTCACCCCGA CAACCGGCCG TCACGAACAC GGACTCTTTA ACCTGTACCA TGCCATGGAT 
GGTGCCAGCC ATCTGCACGT GCTGGTCGTC AAGGAGTATG AAATGGCCAT TTATAAGAAA 
TACTGGCCCA ACCACATCAT GCTGGTGCTC CCAAGTATCT TCAACAGCGC TGGAGTTGGC 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAATC TGGAGCTGGA GAGGAACCGG 
CAGGAGGAGC TGGGGATCAA GCCACAGGAC ATCTGGCCTT TCATCGTGAT CTCGGACGAC 
TCCTGCGTGA TGTGGAACGT GGTGGATGTA GACTGTGGCG GAGAAAGAAG CAGGGAGTTT 
TCCTGGGCAG AAAGAAACGT GTCTTTGAAG CACATCATGC AGCACATCGA GGCGTCCCCC 
AACATCACCC ACTATGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC GGGGAGCGCC 
GAGGTGCGGG AGCCCTTCTC CCGCTGCCAC GTGCACGACT TCATCATTCT GAACGTGGAC 
CTGACCCAGA ACGTGCAGTA CCACCAGAAC CGGTTCACGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ATAGCGCCGG CCTCCTGCTC TGCCGTTTCA ACCGCTTTAG CGTGATGAAG 
AAGCAGATTG CCGTGGGTGG GCACAGGTCC TGCCACATCA CGTCCAAGGT ATCCGACAAC 
CCCGTGGCCA TCGTGCCGGC CCAGTACATC TGCGCCCCGG ACAGCAAGCA CACGTTCCTC 
GCGGCGCCCG CGCAGCTCCT GCTGGAGAAG TTCCTCCAGC ACCACAGTCA CCGATTCTTC 
CCGCTGTCCC TGAGGAACCG CGGCCACCCT GTGCTCTCGG TCGACTGCTA CCTTAACCTG 
GGATCTCAGA TTTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCCCTGAA CATCAGCTGC 
TCTGACTTCG TGTTTAGCGG ACTCCTGCTA TACCTCTGTG ATTCTTTTGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCCTGT GCGTGATCTG CCAGGACCGG 
AACTCCCTGC GCCAGACGGT GGTCCGCCTC GAGCTGGAAG ACGAGTGGCA GTTCCGGCTG 
CGTGACGAAT TCCAGACGGC CAACGCCAAG GAAGACCGGC CGCTCTTTTT TCTGACCGGG 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_027077921.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026933722.1 Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X9, mRNA.

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CloneID	OAc233925
Clone ID Related Accession (Same CDS sequence)	XM_027077916.1 , XM_027077920.1 , XM_027077921.1 , XM_027077917.1 , XM_027077918.1 , XM_027077919.1
Accession Version	XM_027077921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	protein GREB1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834765.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCCGG CCAGCTGAAG ACGACGCGGT TCGAAGAGGT CTTGCACAAC 
TCCATCGAGG CTTCGCTGCG GTCCAACAGC CTGGTGCCCA GGCCCGTCTT CTCCCAGTTG 
TATCTAGAAG CCGAACAGCA GCTCTCCACG CTAGAAGGTG GAGGCCGAGG GGACAATGAG 
GATGAGGAAG AAGAGGGGGA AGGGGCGCTG GAGCCCAGCG GTCCCCCGAA TCCCTACCAG 
ATGCACCCTC CGCCGGAAGG CTGCTGCACC ACAGACGGGT TCTGCCAGGC GGGGAAGGAC 
CTGCGTCTCG TCTCCATTTC CAGTGAGCCC ATAGACGTCC CTGCAGGCTT TCTCCTCGTG 
GGGGCCAAGT CCCCCAGCCT GCCAGACCAT CTCCTGGTGT GTGCTGTGGA CAAAAGGTTC 
TTGCCGGATG ACAATGGGCA CAACGCTCTT CTTGGTTTCT CTGGGAATTG CGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA CTTCACAGAA TTCTCTAATC ACATAAATCT CAAGTTGACC 
ACTCAGCCCA AGAAGCAGAA ACACTTGAAG TATTACCTGG TCCGCAACGC ACAAGGGACT 
CTAACCAAAG GACCTCTCAT CTGTTGGAAA GGCTCAGAGT TCAGAAGCCG GCCGACCTCC 
GCCAGTGCCT GTTCCAGCTC ACTCTTCCCA CCAGCGGAGA GCTCCGGGCC TCTGGCTGCC 
TTCTCCAGCG AGCCCGTTCC CGGAGCCAGC CCGAGCGTCC CCCTGGGAGC CCAGCAGGCA 
GTGCCAGCCT CTGAGCACCC CTCACTTGCT ACGGCCGTGG GTCCAGCTGT TTTCAATGGC 
AGAGATTCTC CGAAGCACCA ACAGCTGGTG AAGAACGACC TATCGGCCAT GCCACGGCCC 
TCGGCATTAG GTATCTTATC AAACTCTGGG CCCCCGAAGA AACGCCACAA AGGGTGGTCC 
CCTGAATCTC CATCAGCGAC AGACGCCGGC TACGCCCAGG GTGGTGGGAA CAGAGCTAAG 
TACGAGAGCG CAGGCATGTC CTGCGTGCCA CAGGTTGGCT CGGTGGGACC AGCGTCGGTC 
ACCTTCCCCG TTGTGGCCTC TGGAGAACCA GTGTCCGTCC CTGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCGGTGAT ATTTAAAGGC CACGGGAACT TCCCTTACCT CTGCGGGAAC 
CTGAGTGACG TCGTCGTGAG CCCCCTCCTG TACACGTGCT ACCAGAACTC TCAGTCCATC 
TCCCGGGCGT ATGAGCACTA CGGGGCCTCG GCGATACAGC CCATCTCCGA GGAGGTGCAG 
CTCCTACTGA CCGTCTACTA TCTCGTCCAG CTGGCAGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCGTGG CGCGAATCGC ACCTGACCGA GATCCGGCAG 
TACCAGCAGG CGCAGCCGCA GCCCTGCCCG CCCGCGCCCA GCGCCGTGGC CCCCGTCACC 
TCGGCCCAGC TGCCCTGGCT CGCCGGCCTG GCCGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC CCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTCATC 
GAGGGCAAGC TCGCCAAGAC CAACTACGTG GTGATCGTCC GCGCGTGCCG CAGCCCCGCC 
ATCGACTCCT GCGTCGCCGT CACGGGAAAA TACCAAGCCC GAATTCTTTC TGAGAGCCTC 
CTCACCCCAG CGGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACCGG GAAAGTGGAC 
TTGTTGGGGG CGTTTTTCAG CACCCTCTGT CCAGAGGGGG ACATTGACAT TTTGCTGGAC 
AAATTTCATC AGGAAAATCA AGGCCATATT TCGTCGTCAC TTACTGCCTC CTCTGTCACC 
AAATCAGCAT CCCTGGATGC ACCGGCATGC ACAAGTTATA ATCTGGAGCC CCACCACATC 
CGGCCTTTCC AGCTGGCCGT GGCTCAGAAG CTCCTCTCCC ATGTGTGTTC AATTGCGGAT 
TCCAGCACCC AGAATTTGGA CTTAGGATCC TTCGAGAAGG TGGACTTTCT GATTTGTATT 
CCACCCTCAG AAGTGACCTA TCAGCAAACC CTGTTCCACG TCTGGCATTC AGGGGTTTTG 
TCAGAGCTCG GCTTGGAAAA GGAGCACATG ACCAAGCAGA GGGTTGAACA GTATGTTTTG 
AAGCTTGATG CTGAAGCGCA GACAAAATTT AAGTCCTTTC TGCAAAACTC CTTTCAGAAT 
CCGCATACGC TCTTTGTCCT GATCCACGAC CACGCCCACT GGGACCTCGT GAGTAGCGCT 
GTTCATAATC TCTATTCCCA AAGTGACCCG GCTGTGGGAT TGGTGGACCG GTTGCTGAAC 
TGCAGAGAGG TGAAGGAGGC CCCGAACATC GTGACGCTTC ACGTGACTTC CTTCCCTTAC 
GCTCTGCAGA CACAGCACAC CCTCATCAGC CCTTACAATG AGATCCACTG GCCCGCCTCC 
TACAGCAATG GGGTGGACTT ATATCCTGAG AACAAGAAGT ACTTCGGGCT GTCAGAGTTC 
ATTGAGTCCA CCCTTTCGGG ACACAGCCTC CCTTTGCTCA GATATGACAG CTCCTTTGAG 
GCCATGGTCA CGGCATTAGG AAAAAGGTTC CCCCGCCTGC ACAGCGCGGT GATCAGGACC 
TTTGTTCTCG TGCAGCACTA CGCGGCCGCC ATGATGGCCG TGAGCGGCCT GTCGCAGATG 
AAGAACTACA CGTCGGTGGA GACGCTGGAG ATCACCCAGA ACCTCATCAA CTCCCCCAAG 
CAGTGCCCCT GCGGCCACGG GCTCATGGTC CTGCTGCGGG TGCCCTGCTC CCCGCTGGCG 
GCGGTGGCCT ACGAGCGGCT GGCCCACGTG CGGGCACGCC TGGCCCTGGA GGAGCACTTT 
GAGATCATCC TGGGCAACCC CAGCTCCGGC ATCACCGTCG GGAAGCACTT CGTGAAGCAA 
CTCAAGCTGT GGCAGAAGAT CGAGGACGCC GAGTGGAGAC CTCAGACCTA CCTGGAGCTG 
GAGGGCCTGC CCTGCATCCT GATCTTCAGT GGGATGGACC CTCACGGGGA ATCCTTACCA 
AGGTCTCTGA GGTACTGTGA CCTGCGTCTG ATAAACTCCT CCTGCTTGGT GAGAACAGCG 
CTGGAGCAGG AGCTGGGCCT GGCCGCTTAC TTTGTGAGCA ACGAGGTTTC TTTGGAGAAG 
GCGGCCCGGA ACGAGCCCTT GGAGAGCGAT GCGGAAAAGC TGAGCAGCAC AGACAACGAG 
GACGAGGAGC TGGTGACGGA AGGCTCCACC TCGGAGAAGA GGAGCCCGGT GAAGCGGGAG 
AGGCCCTGCT CCCATGATTC GGCGGCGTCC TCCCTCTCCT CGAAAGCTTC CGGCTCGGCA 
CCGTGCCGCG AGTCCTCGGC TCAGCCCGGG GCGCCCTCGC AGGGGGAGCG GGCCCGATCA 
CCGCAGCCCT GCGGCCCGTC GGAGGAGGGC AGGGCCCCCG GGGAGAGGCA GAGGCCCCAC 
GCTGGCCGGG GACCGCCGTC GGTCATCAGC AGGCGCAGCC CCGGGCTGGC CGCCCAGCCG 
GACTCCGGCC CCAGGACCAG CCAGAGGAGC GTCCAGGTGT CGGTCACCCC GTCGTCCTCC 
CAGCTGTCCT CCTCCGGCTC GTCGTCCTCG TCCGGGGCGC TCGTCCCGCA GGCCTCCCGG 
TGCTCCCTGA CCAAGGCCTG CCGCCAGCCG CCCGTCGTCT TCCTGCCCAA GCTGGTGTAT 
GACATGGTCA CGGCCACCGA CAGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCC 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGC CCGCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCCC TCTACTACCG GCAGTGGACG GTGCCCCGGC CCAGCCACAT GGACTACAGC 
AACCGCACCG AGGGCCGCGT GGACGGCTTC CACCCGCGCC GGCTGCTGCT CAGCGGGCCC 
CCTCAGGTTG GGAAGACAGG TGCTTACCTG CAGTTCCTTA GTATTTTGTC CAGAATGCTC 
ATCCGGCTGA CGGAAGTGGA TGTTTATGAT GAAGAAGAGA TCAATATCCA CCTCAGAGAG 
GAGTCCGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CAGACCTGGA GCTATTCAAG 
AAGTTGCCTT TTGACTACAT TATTCACGAC CCGAAGTATG AAGACGCCAG TCTGATCTGT 
TCGCACCTTC AGAGCGTGAA GAGTGAAGCC AGGGGGACGT CCCGGAAGCC GGAGGAGCTC 
TACGTGCGGC GCCAGACAGC GCGGATGAGG CTGTCCAAGT ACGCGGCCTA TAACACATAC 
CACCACTGCG AGCAGTGTCA CCACTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTACAT 
TTCATCATCC CCAAGTCCAA AGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GGCTGCCCCT GGTCACAGAC AAGAGCCACG AGTATATAAA AAGTCCGACG 
TTCACCCCGA CAACCGGCCG TCACGAACAC GGACTCTTTA ACCTGTACCA TGCCATGGAT 
GGTGCCAGCC ATCTGCACGT GCTGGTCGTC AAGGAGTATG AAATGGCCAT TTATAAGAAA 
TACTGGCCCA ACCACATCAT GCTGGTGCTC CCAAGTATCT TCAACAGCGC TGGAGTTGGC 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAATC TGGAGCTGGA GAGGAACCGG 
CAGGAGGAGC TGGGGATCAA GCCACAGGAC ATCTGGCCTT TCATCGTGAT CTCGGACGAC 
TCCTGCGTGA TGTGGAACGT GGTGGATGTA GACTGTGGCG GAGAAAGAAG CAGGGAGTTT 
TCCTGGGCAG AAAGAAACGT GTCTTTGAAG CACATCATGC AGCACATCGA GGCGTCCCCC 
AACATCACCC ACTATGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC GGGGAGCGCC 
GAGGTGCGGG AGCCCTTCTC CCGCTGCCAC GTGCACGACT TCATCATTCT GAACGTGGAC 
CTGACCCAGA ACGTGCAGTA CCACCAGAAC CGGTTCACGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ATAGCGCCGG CCTCCTGCTC TGCCGTTTCA ACCGCTTTAG CGTGATGAAG 
AAGCAGATTG CCGTGGGTGG GCACAGGTCC TGCCACATCA CGTCCAAGGT ATCCGACAAC 
CCCGTGGCCA TCGTGCCGGC CCAGTACATC TGCGCCCCGG ACAGCAAGCA CACGTTCCTC 
GCGGCGCCCG CGCAGCTCCT GCTGGAGAAG TTCCTCCAGC ACCACAGTCA CCGATTCTTC 
CCGCTGTCCC TGAGGAACCG CGGCCACCCT GTGCTCTCGG TCGACTGCTA CCTTAACCTG 
GGATCTCAGA TTTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCCCTGAA CATCAGCTGC 
TCTGACTTCG TGTTTAGCGG ACTCCTGCTA TACCTCTGTG ATTCTTTTGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCCTGT GCGTGATCTG CCAGGACCGG 
AACTCCCTGC GCCAGACGGT GGTCCGCCTC GAGCTGGAAG ACGAGTGGCA GTTCCGGCTG 
CGTGACGAAT TCCAGACGGC CAACGCCAAG GAAGACCGGC CGCTCTTTTT TCTGACCGGG 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026933722.1
CDS	168..5999
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPVFSQLYLEAEQQLSTLEGGGRGDNEDEEEEGEGALEPSGPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGTLTKGPLICWKGSEFRSRPTSASACSSSLFPPAESSGPLAAFSSEPVPGASPSVPLGAQQAVPASEHPSLATAVGPAVFNGRDSPKHQQLVKNDLSAMPRPSALGILSNSGPPKKRHKGWSPESPSATDAGYAQGGGNRAKYESAGMSCVPQVGSVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLSDVVVSPLLYTCYQNSQSISRAYEHYGASAIQPISEEVQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAQPQPCPPAPSAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIVRACRSPAIDSCVAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDLLGAFFSTLCPEGDIDILLDKFHQENQGHISSSLTASSVTKSASLDAPACTSYNLEPHHIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLSELGLEKEHMTKQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPAVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPASYSNGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPSSGITVGKHFVKQLKLWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNEVSLEKAARNEPLESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERPCSHDSAASSLSSKASGSAPCRESSAQPGAPSQGERARSPQPCGPSEEGRAPGERQRPHAGRGPPSVISRRSPGLAAQPDSGPRTSQRSVQVSVTPSSSQLSSSGSSSSSGALVPQASRCSLTKACRQPPVVFLPKLVYDMVTATDSSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPSHMDYSNRTEGRVDGFHPRRLLLSGPPQVGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINIHLREESDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHLQSVKSEARGTSRKPEELYVRRQTARMRLSKYAAYNTYHHCEQCHHYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDCGGERSREFSWAERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSAEVREPFSRCHVHDFIILNVDLTQNVQYHQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSCHITSKVSDNPVAIVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLRNRGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_027077921.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027077921.1

ORF Insert Sequence:

ATGGGAAACT CGTACGCCGG CCAGCTGAAG ACGACGCGGT TCGAAGAGGT CTTGCACAAC 
TCCATCGAGG CTTCGCTGCG GTCCAACAGC CTGGTGCCCA GGCCCGTCTT CTCCCAGTTG 
TATCTAGAAG CCGAACAGCA GCTCTCCACG CTAGAAGGTG GAGGCCGAGG GGACAATGAG 
GATGAGGAAG AAGAGGGGGA AGGGGCGCTG GAGCCCAGCG GTCCCCCGAA TCCCTACCAG 
ATGCACCCTC CGCCGGAAGG CTGCTGCACC ACAGACGGGT TCTGCCAGGC GGGGAAGGAC 
CTGCGTCTCG TCTCCATTTC CAGTGAGCCC ATAGACGTCC CTGCAGGCTT TCTCCTCGTG 
GGGGCCAAGT CCCCCAGCCT GCCAGACCAT CTCCTGGTGT GTGCTGTGGA CAAAAGGTTC 
TTGCCGGATG ACAATGGGCA CAACGCTCTT CTTGGTTTCT CTGGGAATTG CGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA CTTCACAGAA TTCTCTAATC ACATAAATCT CAAGTTGACC 
ACTCAGCCCA AGAAGCAGAA ACACTTGAAG TATTACCTGG TCCGCAACGC ACAAGGGACT 
CTAACCAAAG GACCTCTCAT CTGTTGGAAA GGCTCAGAGT TCAGAAGCCG GCCGACCTCC 
GCCAGTGCCT GTTCCAGCTC ACTCTTCCCA CCAGCGGAGA GCTCCGGGCC TCTGGCTGCC 
TTCTCCAGCG AGCCCGTTCC CGGAGCCAGC CCGAGCGTCC CCCTGGGAGC CCAGCAGGCA 
GTGCCAGCCT CTGAGCACCC CTCACTTGCT ACGGCCGTGG GTCCAGCTGT TTTCAATGGC 
AGAGATTCTC CGAAGCACCA ACAGCTGGTG AAGAACGACC TATCGGCCAT GCCACGGCCC 
TCGGCATTAG GTATCTTATC AAACTCTGGG CCCCCGAAGA AACGCCACAA AGGGTGGTCC 
CCTGAATCTC CATCAGCGAC AGACGCCGGC TACGCCCAGG GTGGTGGGAA CAGAGCTAAG 
TACGAGAGCG CAGGCATGTC CTGCGTGCCA CAGGTTGGCT CGGTGGGACC AGCGTCGGTC 
ACCTTCCCCG TTGTGGCCTC TGGAGAACCA GTGTCCGTCC CTGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCGGTGAT ATTTAAAGGC CACGGGAACT TCCCTTACCT CTGCGGGAAC 
CTGAGTGACG TCGTCGTGAG CCCCCTCCTG TACACGTGCT ACCAGAACTC TCAGTCCATC 
TCCCGGGCGT ATGAGCACTA CGGGGCCTCG GCGATACAGC CCATCTCCGA GGAGGTGCAG 
CTCCTACTGA CCGTCTACTA TCTCGTCCAG CTGGCAGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCGTGG CGCGAATCGC ACCTGACCGA GATCCGGCAG 
TACCAGCAGG CGCAGCCGCA GCCCTGCCCG CCCGCGCCCA GCGCCGTGGC CCCCGTCACC 
TCGGCCCAGC TGCCCTGGCT CGCCGGCCTG GCCGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC CCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTCATC 
GAGGGCAAGC TCGCCAAGAC CAACTACGTG GTGATCGTCC GCGCGTGCCG CAGCCCCGCC 
ATCGACTCCT GCGTCGCCGT CACGGGAAAA TACCAAGCCC GAATTCTTTC TGAGAGCCTC 
CTCACCCCAG CGGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACCGG GAAAGTGGAC 
TTGTTGGGGG CGTTTTTCAG CACCCTCTGT CCAGAGGGGG ACATTGACAT TTTGCTGGAC 
AAATTTCATC AGGAAAATCA AGGCCATATT TCGTCGTCAC TTACTGCCTC CTCTGTCACC 
AAATCAGCAT CCCTGGATGC ACCGGCATGC ACAAGTTATA ATCTGGAGCC CCACCACATC 
CGGCCTTTCC AGCTGGCCGT GGCTCAGAAG CTCCTCTCCC ATGTGTGTTC AATTGCGGAT 
TCCAGCACCC AGAATTTGGA CTTAGGATCC TTCGAGAAGG TGGACTTTCT GATTTGTATT 
CCACCCTCAG AAGTGACCTA TCAGCAAACC CTGTTCCACG TCTGGCATTC AGGGGTTTTG 
TCAGAGCTCG GCTTGGAAAA GGAGCACATG ACCAAGCAGA GGGTTGAACA GTATGTTTTG 
AAGCTTGATG CTGAAGCGCA GACAAAATTT AAGTCCTTTC TGCAAAACTC CTTTCAGAAT 
CCGCATACGC TCTTTGTCCT GATCCACGAC CACGCCCACT GGGACCTCGT GAGTAGCGCT 
GTTCATAATC TCTATTCCCA AAGTGACCCG GCTGTGGGAT TGGTGGACCG GTTGCTGAAC 
TGCAGAGAGG TGAAGGAGGC CCCGAACATC GTGACGCTTC ACGTGACTTC CTTCCCTTAC 
GCTCTGCAGA CACAGCACAC CCTCATCAGC CCTTACAATG AGATCCACTG GCCCGCCTCC 
TACAGCAATG GGGTGGACTT ATATCCTGAG AACAAGAAGT ACTTCGGGCT GTCAGAGTTC 
ATTGAGTCCA CCCTTTCGGG ACACAGCCTC CCTTTGCTCA GATATGACAG CTCCTTTGAG 
GCCATGGTCA CGGCATTAGG AAAAAGGTTC CCCCGCCTGC ACAGCGCGGT GATCAGGACC 
TTTGTTCTCG TGCAGCACTA CGCGGCCGCC ATGATGGCCG TGAGCGGCCT GTCGCAGATG 
AAGAACTACA CGTCGGTGGA GACGCTGGAG ATCACCCAGA ACCTCATCAA CTCCCCCAAG 
CAGTGCCCCT GCGGCCACGG GCTCATGGTC CTGCTGCGGG TGCCCTGCTC CCCGCTGGCG 
GCGGTGGCCT ACGAGCGGCT GGCCCACGTG CGGGCACGCC TGGCCCTGGA GGAGCACTTT 
GAGATCATCC TGGGCAACCC CAGCTCCGGC ATCACCGTCG GGAAGCACTT CGTGAAGCAA 
CTCAAGCTGT GGCAGAAGAT CGAGGACGCC GAGTGGAGAC CTCAGACCTA CCTGGAGCTG 
GAGGGCCTGC CCTGCATCCT GATCTTCAGT GGGATGGACC CTCACGGGGA ATCCTTACCA 
AGGTCTCTGA GGTACTGTGA CCTGCGTCTG ATAAACTCCT CCTGCTTGGT GAGAACAGCG 
CTGGAGCAGG AGCTGGGCCT GGCCGCTTAC TTTGTGAGCA ACGAGGTTTC TTTGGAGAAG 
GCGGCCCGGA ACGAGCCCTT GGAGAGCGAT GCGGAAAAGC TGAGCAGCAC AGACAACGAG 
GACGAGGAGC TGGTGACGGA AGGCTCCACC TCGGAGAAGA GGAGCCCGGT GAAGCGGGAG 
AGGCCCTGCT CCCATGATTC GGCGGCGTCC TCCCTCTCCT CGAAAGCTTC CGGCTCGGCA 
CCGTGCCGCG AGTCCTCGGC TCAGCCCGGG GCGCCCTCGC AGGGGGAGCG GGCCCGATCA 
CCGCAGCCCT GCGGCCCGTC GGAGGAGGGC AGGGCCCCCG GGGAGAGGCA GAGGCCCCAC 
GCTGGCCGGG GACCGCCGTC GGTCATCAGC AGGCGCAGCC CCGGGCTGGC CGCCCAGCCG 
GACTCCGGCC CCAGGACCAG CCAGAGGAGC GTCCAGGTGT CGGTCACCCC GTCGTCCTCC 
CAGCTGTCCT CCTCCGGCTC GTCGTCCTCG TCCGGGGCGC TCGTCCCGCA GGCCTCCCGG 
TGCTCCCTGA CCAAGGCCTG CCGCCAGCCG CCCGTCGTCT TCCTGCCCAA GCTGGTGTAT 
GACATGGTCA CGGCCACCGA CAGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCC 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGC CCGCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCCC TCTACTACCG GCAGTGGACG GTGCCCCGGC CCAGCCACAT GGACTACAGC 
AACCGCACCG AGGGCCGCGT GGACGGCTTC CACCCGCGCC GGCTGCTGCT CAGCGGGCCC 
CCTCAGGTTG GGAAGACAGG TGCTTACCTG CAGTTCCTTA GTATTTTGTC CAGAATGCTC 
ATCCGGCTGA CGGAAGTGGA TGTTTATGAT GAAGAAGAGA TCAATATCCA CCTCAGAGAG 
GAGTCCGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CAGACCTGGA GCTATTCAAG 
AAGTTGCCTT TTGACTACAT TATTCACGAC CCGAAGTATG AAGACGCCAG TCTGATCTGT 
TCGCACCTTC AGAGCGTGAA GAGTGAAGCC AGGGGGACGT CCCGGAAGCC GGAGGAGCTC 
TACGTGCGGC GCCAGACAGC GCGGATGAGG CTGTCCAAGT ACGCGGCCTA TAACACATAC 
CACCACTGCG AGCAGTGTCA CCACTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTACAT 
TTCATCATCC CCAAGTCCAA AGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GGCTGCCCCT GGTCACAGAC AAGAGCCACG AGTATATAAA AAGTCCGACG 
TTCACCCCGA CAACCGGCCG TCACGAACAC GGACTCTTTA ACCTGTACCA TGCCATGGAT 
GGTGCCAGCC ATCTGCACGT GCTGGTCGTC AAGGAGTATG AAATGGCCAT TTATAAGAAA 
TACTGGCCCA ACCACATCAT GCTGGTGCTC CCAAGTATCT TCAACAGCGC TGGAGTTGGC 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAATC TGGAGCTGGA GAGGAACCGG 
CAGGAGGAGC TGGGGATCAA GCCACAGGAC ATCTGGCCTT TCATCGTGAT CTCGGACGAC 
TCCTGCGTGA TGTGGAACGT GGTGGATGTA GACTGTGGCG GAGAAAGAAG CAGGGAGTTT 
TCCTGGGCAG AAAGAAACGT GTCTTTGAAG CACATCATGC AGCACATCGA GGCGTCCCCC 
AACATCACCC ACTATGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC GGGGAGCGCC 
GAGGTGCGGG AGCCCTTCTC CCGCTGCCAC GTGCACGACT TCATCATTCT GAACGTGGAC 
CTGACCCAGA ACGTGCAGTA CCACCAGAAC CGGTTCACGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ATAGCGCCGG CCTCCTGCTC TGCCGTTTCA ACCGCTTTAG CGTGATGAAG 
AAGCAGATTG CCGTGGGTGG GCACAGGTCC TGCCACATCA CGTCCAAGGT ATCCGACAAC 
CCCGTGGCCA TCGTGCCGGC CCAGTACATC TGCGCCCCGG ACAGCAAGCA CACGTTCCTC 
GCGGCGCCCG CGCAGCTCCT GCTGGAGAAG TTCCTCCAGC ACCACAGTCA CCGATTCTTC 
CCGCTGTCCC TGAGGAACCG CGGCCACCCT GTGCTCTCGG TCGACTGCTA CCTTAACCTG 
GGATCTCAGA TTTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCCCTGAA CATCAGCTGC 
TCTGACTTCG TGTTTAGCGG ACTCCTGCTA TACCTCTGTG ATTCTTTTGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCCTGT GCGTGATCTG CCAGGACCGG 
AACTCCCTGC GCCAGACGGT GGTCCGCCTC GAGCTGGAAG ACGAGTGGCA GTTCCGGCTG 
CGTGACGAAT TCCAGACGGC CAACGCCAAG GAAGACCGGC CGCTCTTTTT TCTGACCGGG 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_027077917.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026933718.1 Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X5, mRNA.

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CloneID	OAc233925
Clone ID Related Accession (Same CDS sequence)	XM_027077916.1 , XM_027077920.1 , XM_027077921.1 , XM_027077917.1 , XM_027077918.1 , XM_027077919.1
Accession Version	XM_027077917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	protein GREB1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834765.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCCGG CCAGCTGAAG ACGACGCGGT TCGAAGAGGT CTTGCACAAC 
TCCATCGAGG CTTCGCTGCG GTCCAACAGC CTGGTGCCCA GGCCCGTCTT CTCCCAGTTG 
TATCTAGAAG CCGAACAGCA GCTCTCCACG CTAGAAGGTG GAGGCCGAGG GGACAATGAG 
GATGAGGAAG AAGAGGGGGA AGGGGCGCTG GAGCCCAGCG GTCCCCCGAA TCCCTACCAG 
ATGCACCCTC CGCCGGAAGG CTGCTGCACC ACAGACGGGT TCTGCCAGGC GGGGAAGGAC 
CTGCGTCTCG TCTCCATTTC CAGTGAGCCC ATAGACGTCC CTGCAGGCTT TCTCCTCGTG 
GGGGCCAAGT CCCCCAGCCT GCCAGACCAT CTCCTGGTGT GTGCTGTGGA CAAAAGGTTC 
TTGCCGGATG ACAATGGGCA CAACGCTCTT CTTGGTTTCT CTGGGAATTG CGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA CTTCACAGAA TTCTCTAATC ACATAAATCT CAAGTTGACC 
ACTCAGCCCA AGAAGCAGAA ACACTTGAAG TATTACCTGG TCCGCAACGC ACAAGGGACT 
CTAACCAAAG GACCTCTCAT CTGTTGGAAA GGCTCAGAGT TCAGAAGCCG GCCGACCTCC 
GCCAGTGCCT GTTCCAGCTC ACTCTTCCCA CCAGCGGAGA GCTCCGGGCC TCTGGCTGCC 
TTCTCCAGCG AGCCCGTTCC CGGAGCCAGC CCGAGCGTCC CCCTGGGAGC CCAGCAGGCA 
GTGCCAGCCT CTGAGCACCC CTCACTTGCT ACGGCCGTGG GTCCAGCTGT TTTCAATGGC 
AGAGATTCTC CGAAGCACCA ACAGCTGGTG AAGAACGACC TATCGGCCAT GCCACGGCCC 
TCGGCATTAG GTATCTTATC AAACTCTGGG CCCCCGAAGA AACGCCACAA AGGGTGGTCC 
CCTGAATCTC CATCAGCGAC AGACGCCGGC TACGCCCAGG GTGGTGGGAA CAGAGCTAAG 
TACGAGAGCG CAGGCATGTC CTGCGTGCCA CAGGTTGGCT CGGTGGGACC AGCGTCGGTC 
ACCTTCCCCG TTGTGGCCTC TGGAGAACCA GTGTCCGTCC CTGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCGGTGAT ATTTAAAGGC CACGGGAACT TCCCTTACCT CTGCGGGAAC 
CTGAGTGACG TCGTCGTGAG CCCCCTCCTG TACACGTGCT ACCAGAACTC TCAGTCCATC 
TCCCGGGCGT ATGAGCACTA CGGGGCCTCG GCGATACAGC CCATCTCCGA GGAGGTGCAG 
CTCCTACTGA CCGTCTACTA TCTCGTCCAG CTGGCAGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCGTGG CGCGAATCGC ACCTGACCGA GATCCGGCAG 
TACCAGCAGG CGCAGCCGCA GCCCTGCCCG CCCGCGCCCA GCGCCGTGGC CCCCGTCACC 
TCGGCCCAGC TGCCCTGGCT CGCCGGCCTG GCCGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC CCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTCATC 
GAGGGCAAGC TCGCCAAGAC CAACTACGTG GTGATCGTCC GCGCGTGCCG CAGCCCCGCC 
ATCGACTCCT GCGTCGCCGT CACGGGAAAA TACCAAGCCC GAATTCTTTC TGAGAGCCTC 
CTCACCCCAG CGGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACCGG GAAAGTGGAC 
TTGTTGGGGG CGTTTTTCAG CACCCTCTGT CCAGAGGGGG ACATTGACAT TTTGCTGGAC 
AAATTTCATC AGGAAAATCA AGGCCATATT TCGTCGTCAC TTACTGCCTC CTCTGTCACC 
AAATCAGCAT CCCTGGATGC ACCGGCATGC ACAAGTTATA ATCTGGAGCC CCACCACATC 
CGGCCTTTCC AGCTGGCCGT GGCTCAGAAG CTCCTCTCCC ATGTGTGTTC AATTGCGGAT 
TCCAGCACCC AGAATTTGGA CTTAGGATCC TTCGAGAAGG TGGACTTTCT GATTTGTATT 
CCACCCTCAG AAGTGACCTA TCAGCAAACC CTGTTCCACG TCTGGCATTC AGGGGTTTTG 
TCAGAGCTCG GCTTGGAAAA GGAGCACATG ACCAAGCAGA GGGTTGAACA GTATGTTTTG 
AAGCTTGATG CTGAAGCGCA GACAAAATTT AAGTCCTTTC TGCAAAACTC CTTTCAGAAT 
CCGCATACGC TCTTTGTCCT GATCCACGAC CACGCCCACT GGGACCTCGT GAGTAGCGCT 
GTTCATAATC TCTATTCCCA AAGTGACCCG GCTGTGGGAT TGGTGGACCG GTTGCTGAAC 
TGCAGAGAGG TGAAGGAGGC CCCGAACATC GTGACGCTTC ACGTGACTTC CTTCCCTTAC 
GCTCTGCAGA CACAGCACAC CCTCATCAGC CCTTACAATG AGATCCACTG GCCCGCCTCC 
TACAGCAATG GGGTGGACTT ATATCCTGAG AACAAGAAGT ACTTCGGGCT GTCAGAGTTC 
ATTGAGTCCA CCCTTTCGGG ACACAGCCTC CCTTTGCTCA GATATGACAG CTCCTTTGAG 
GCCATGGTCA CGGCATTAGG AAAAAGGTTC CCCCGCCTGC ACAGCGCGGT GATCAGGACC 
TTTGTTCTCG TGCAGCACTA CGCGGCCGCC ATGATGGCCG TGAGCGGCCT GTCGCAGATG 
AAGAACTACA CGTCGGTGGA GACGCTGGAG ATCACCCAGA ACCTCATCAA CTCCCCCAAG 
CAGTGCCCCT GCGGCCACGG GCTCATGGTC CTGCTGCGGG TGCCCTGCTC CCCGCTGGCG 
GCGGTGGCCT ACGAGCGGCT GGCCCACGTG CGGGCACGCC TGGCCCTGGA GGAGCACTTT 
GAGATCATCC TGGGCAACCC CAGCTCCGGC ATCACCGTCG GGAAGCACTT CGTGAAGCAA 
CTCAAGCTGT GGCAGAAGAT CGAGGACGCC GAGTGGAGAC CTCAGACCTA CCTGGAGCTG 
GAGGGCCTGC CCTGCATCCT GATCTTCAGT GGGATGGACC CTCACGGGGA ATCCTTACCA 
AGGTCTCTGA GGTACTGTGA CCTGCGTCTG ATAAACTCCT CCTGCTTGGT GAGAACAGCG 
CTGGAGCAGG AGCTGGGCCT GGCCGCTTAC TTTGTGAGCA ACGAGGTTTC TTTGGAGAAG 
GCGGCCCGGA ACGAGCCCTT GGAGAGCGAT GCGGAAAAGC TGAGCAGCAC AGACAACGAG 
GACGAGGAGC TGGTGACGGA AGGCTCCACC TCGGAGAAGA GGAGCCCGGT GAAGCGGGAG 
AGGCCCTGCT CCCATGATTC GGCGGCGTCC TCCCTCTCCT CGAAAGCTTC CGGCTCGGCA 
CCGTGCCGCG AGTCCTCGGC TCAGCCCGGG GCGCCCTCGC AGGGGGAGCG GGCCCGATCA 
CCGCAGCCCT GCGGCCCGTC GGAGGAGGGC AGGGCCCCCG GGGAGAGGCA GAGGCCCCAC 
GCTGGCCGGG GACCGCCGTC GGTCATCAGC AGGCGCAGCC CCGGGCTGGC CGCCCAGCCG 
GACTCCGGCC CCAGGACCAG CCAGAGGAGC GTCCAGGTGT CGGTCACCCC GTCGTCCTCC 
CAGCTGTCCT CCTCCGGCTC GTCGTCCTCG TCCGGGGCGC TCGTCCCGCA GGCCTCCCGG 
TGCTCCCTGA CCAAGGCCTG CCGCCAGCCG CCCGTCGTCT TCCTGCCCAA GCTGGTGTAT 
GACATGGTCA CGGCCACCGA CAGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCC 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGC CCGCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCCC TCTACTACCG GCAGTGGACG GTGCCCCGGC CCAGCCACAT GGACTACAGC 
AACCGCACCG AGGGCCGCGT GGACGGCTTC CACCCGCGCC GGCTGCTGCT CAGCGGGCCC 
CCTCAGGTTG GGAAGACAGG TGCTTACCTG CAGTTCCTTA GTATTTTGTC CAGAATGCTC 
ATCCGGCTGA CGGAAGTGGA TGTTTATGAT GAAGAAGAGA TCAATATCCA CCTCAGAGAG 
GAGTCCGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CAGACCTGGA GCTATTCAAG 
AAGTTGCCTT TTGACTACAT TATTCACGAC CCGAAGTATG AAGACGCCAG TCTGATCTGT 
TCGCACCTTC AGAGCGTGAA GAGTGAAGCC AGGGGGACGT CCCGGAAGCC GGAGGAGCTC 
TACGTGCGGC GCCAGACAGC GCGGATGAGG CTGTCCAAGT ACGCGGCCTA TAACACATAC 
CACCACTGCG AGCAGTGTCA CCACTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTACAT 
TTCATCATCC CCAAGTCCAA AGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GGCTGCCCCT GGTCACAGAC AAGAGCCACG AGTATATAAA AAGTCCGACG 
TTCACCCCGA CAACCGGCCG TCACGAACAC GGACTCTTTA ACCTGTACCA TGCCATGGAT 
GGTGCCAGCC ATCTGCACGT GCTGGTCGTC AAGGAGTATG AAATGGCCAT TTATAAGAAA 
TACTGGCCCA ACCACATCAT GCTGGTGCTC CCAAGTATCT TCAACAGCGC TGGAGTTGGC 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAATC TGGAGCTGGA GAGGAACCGG 
CAGGAGGAGC TGGGGATCAA GCCACAGGAC ATCTGGCCTT TCATCGTGAT CTCGGACGAC 
TCCTGCGTGA TGTGGAACGT GGTGGATGTA GACTGTGGCG GAGAAAGAAG CAGGGAGTTT 
TCCTGGGCAG AAAGAAACGT GTCTTTGAAG CACATCATGC AGCACATCGA GGCGTCCCCC 
AACATCACCC ACTATGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC GGGGAGCGCC 
GAGGTGCGGG AGCCCTTCTC CCGCTGCCAC GTGCACGACT TCATCATTCT GAACGTGGAC 
CTGACCCAGA ACGTGCAGTA CCACCAGAAC CGGTTCACGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ATAGCGCCGG CCTCCTGCTC TGCCGTTTCA ACCGCTTTAG CGTGATGAAG 
AAGCAGATTG CCGTGGGTGG GCACAGGTCC TGCCACATCA CGTCCAAGGT ATCCGACAAC 
CCCGTGGCCA TCGTGCCGGC CCAGTACATC TGCGCCCCGG ACAGCAAGCA CACGTTCCTC 
GCGGCGCCCG CGCAGCTCCT GCTGGAGAAG TTCCTCCAGC ACCACAGTCA CCGATTCTTC 
CCGCTGTCCC TGAGGAACCG CGGCCACCCT GTGCTCTCGG TCGACTGCTA CCTTAACCTG 
GGATCTCAGA TTTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCCCTGAA CATCAGCTGC 
TCTGACTTCG TGTTTAGCGG ACTCCTGCTA TACCTCTGTG ATTCTTTTGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCCTGT GCGTGATCTG CCAGGACCGG 
AACTCCCTGC GCCAGACGGT GGTCCGCCTC GAGCTGGAAG ACGAGTGGCA GTTCCGGCTG 
CGTGACGAAT TCCAGACGGC CAACGCCAAG GAAGACCGGC CGCTCTTTTT TCTGACCGGG 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026933718.1
CDS	706..6537
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPVFSQLYLEAEQQLSTLEGGGRGDNEDEEEEGEGALEPSGPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGTLTKGPLICWKGSEFRSRPTSASACSSSLFPPAESSGPLAAFSSEPVPGASPSVPLGAQQAVPASEHPSLATAVGPAVFNGRDSPKHQQLVKNDLSAMPRPSALGILSNSGPPKKRHKGWSPESPSATDAGYAQGGGNRAKYESAGMSCVPQVGSVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLSDVVVSPLLYTCYQNSQSISRAYEHYGASAIQPISEEVQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAQPQPCPPAPSAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIVRACRSPAIDSCVAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDLLGAFFSTLCPEGDIDILLDKFHQENQGHISSSLTASSVTKSASLDAPACTSYNLEPHHIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLSELGLEKEHMTKQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPAVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPASYSNGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPSSGITVGKHFVKQLKLWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNEVSLEKAARNEPLESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERPCSHDSAASSLSSKASGSAPCRESSAQPGAPSQGERARSPQPCGPSEEGRAPGERQRPHAGRGPPSVISRRSPGLAAQPDSGPRTSQRSVQVSVTPSSSQLSSSGSSSSSGALVPQASRCSLTKACRQPPVVFLPKLVYDMVTATDSSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPSHMDYSNRTEGRVDGFHPRRLLLSGPPQVGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINIHLREESDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHLQSVKSEARGTSRKPEELYVRRQTARMRLSKYAAYNTYHHCEQCHHYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDCGGERSREFSWAERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSAEVREPFSRCHVHDFIILNVDLTQNVQYHQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSCHITSKVSDNPVAIVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLRNRGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_027077917.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027077917.1

ORF Insert Sequence:

ATGGGAAACT CGTACGCCGG CCAGCTGAAG ACGACGCGGT TCGAAGAGGT CTTGCACAAC 
TCCATCGAGG CTTCGCTGCG GTCCAACAGC CTGGTGCCCA GGCCCGTCTT CTCCCAGTTG 
TATCTAGAAG CCGAACAGCA GCTCTCCACG CTAGAAGGTG GAGGCCGAGG GGACAATGAG 
GATGAGGAAG AAGAGGGGGA AGGGGCGCTG GAGCCCAGCG GTCCCCCGAA TCCCTACCAG 
ATGCACCCTC CGCCGGAAGG CTGCTGCACC ACAGACGGGT TCTGCCAGGC GGGGAAGGAC 
CTGCGTCTCG TCTCCATTTC CAGTGAGCCC ATAGACGTCC CTGCAGGCTT TCTCCTCGTG 
GGGGCCAAGT CCCCCAGCCT GCCAGACCAT CTCCTGGTGT GTGCTGTGGA CAAAAGGTTC 
TTGCCGGATG ACAATGGGCA CAACGCTCTT CTTGGTTTCT CTGGGAATTG CGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA CTTCACAGAA TTCTCTAATC ACATAAATCT CAAGTTGACC 
ACTCAGCCCA AGAAGCAGAA ACACTTGAAG TATTACCTGG TCCGCAACGC ACAAGGGACT 
CTAACCAAAG GACCTCTCAT CTGTTGGAAA GGCTCAGAGT TCAGAAGCCG GCCGACCTCC 
GCCAGTGCCT GTTCCAGCTC ACTCTTCCCA CCAGCGGAGA GCTCCGGGCC TCTGGCTGCC 
TTCTCCAGCG AGCCCGTTCC CGGAGCCAGC CCGAGCGTCC CCCTGGGAGC CCAGCAGGCA 
GTGCCAGCCT CTGAGCACCC CTCACTTGCT ACGGCCGTGG GTCCAGCTGT TTTCAATGGC 
AGAGATTCTC CGAAGCACCA ACAGCTGGTG AAGAACGACC TATCGGCCAT GCCACGGCCC 
TCGGCATTAG GTATCTTATC AAACTCTGGG CCCCCGAAGA AACGCCACAA AGGGTGGTCC 
CCTGAATCTC CATCAGCGAC AGACGCCGGC TACGCCCAGG GTGGTGGGAA CAGAGCTAAG 
TACGAGAGCG CAGGCATGTC CTGCGTGCCA CAGGTTGGCT CGGTGGGACC AGCGTCGGTC 
ACCTTCCCCG TTGTGGCCTC TGGAGAACCA GTGTCCGTCC CTGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCGGTGAT ATTTAAAGGC CACGGGAACT TCCCTTACCT CTGCGGGAAC 
CTGAGTGACG TCGTCGTGAG CCCCCTCCTG TACACGTGCT ACCAGAACTC TCAGTCCATC 
TCCCGGGCGT ATGAGCACTA CGGGGCCTCG GCGATACAGC CCATCTCCGA GGAGGTGCAG 
CTCCTACTGA CCGTCTACTA TCTCGTCCAG CTGGCAGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCGTGG CGCGAATCGC ACCTGACCGA GATCCGGCAG 
TACCAGCAGG CGCAGCCGCA GCCCTGCCCG CCCGCGCCCA GCGCCGTGGC CCCCGTCACC 
TCGGCCCAGC TGCCCTGGCT CGCCGGCCTG GCCGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC CCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTCATC 
GAGGGCAAGC TCGCCAAGAC CAACTACGTG GTGATCGTCC GCGCGTGCCG CAGCCCCGCC 
ATCGACTCCT GCGTCGCCGT CACGGGAAAA TACCAAGCCC GAATTCTTTC TGAGAGCCTC 
CTCACCCCAG CGGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACCGG GAAAGTGGAC 
TTGTTGGGGG CGTTTTTCAG CACCCTCTGT CCAGAGGGGG ACATTGACAT TTTGCTGGAC 
AAATTTCATC AGGAAAATCA AGGCCATATT TCGTCGTCAC TTACTGCCTC CTCTGTCACC 
AAATCAGCAT CCCTGGATGC ACCGGCATGC ACAAGTTATA ATCTGGAGCC CCACCACATC 
CGGCCTTTCC AGCTGGCCGT GGCTCAGAAG CTCCTCTCCC ATGTGTGTTC AATTGCGGAT 
TCCAGCACCC AGAATTTGGA CTTAGGATCC TTCGAGAAGG TGGACTTTCT GATTTGTATT 
CCACCCTCAG AAGTGACCTA TCAGCAAACC CTGTTCCACG TCTGGCATTC AGGGGTTTTG 
TCAGAGCTCG GCTTGGAAAA GGAGCACATG ACCAAGCAGA GGGTTGAACA GTATGTTTTG 
AAGCTTGATG CTGAAGCGCA GACAAAATTT AAGTCCTTTC TGCAAAACTC CTTTCAGAAT 
CCGCATACGC TCTTTGTCCT GATCCACGAC CACGCCCACT GGGACCTCGT GAGTAGCGCT 
GTTCATAATC TCTATTCCCA AAGTGACCCG GCTGTGGGAT TGGTGGACCG GTTGCTGAAC 
TGCAGAGAGG TGAAGGAGGC CCCGAACATC GTGACGCTTC ACGTGACTTC CTTCCCTTAC 
GCTCTGCAGA CACAGCACAC CCTCATCAGC CCTTACAATG AGATCCACTG GCCCGCCTCC 
TACAGCAATG GGGTGGACTT ATATCCTGAG AACAAGAAGT ACTTCGGGCT GTCAGAGTTC 
ATTGAGTCCA CCCTTTCGGG ACACAGCCTC CCTTTGCTCA GATATGACAG CTCCTTTGAG 
GCCATGGTCA CGGCATTAGG AAAAAGGTTC CCCCGCCTGC ACAGCGCGGT GATCAGGACC 
TTTGTTCTCG TGCAGCACTA CGCGGCCGCC ATGATGGCCG TGAGCGGCCT GTCGCAGATG 
AAGAACTACA CGTCGGTGGA GACGCTGGAG ATCACCCAGA ACCTCATCAA CTCCCCCAAG 
CAGTGCCCCT GCGGCCACGG GCTCATGGTC CTGCTGCGGG TGCCCTGCTC CCCGCTGGCG 
GCGGTGGCCT ACGAGCGGCT GGCCCACGTG CGGGCACGCC TGGCCCTGGA GGAGCACTTT 
GAGATCATCC TGGGCAACCC CAGCTCCGGC ATCACCGTCG GGAAGCACTT CGTGAAGCAA 
CTCAAGCTGT GGCAGAAGAT CGAGGACGCC GAGTGGAGAC CTCAGACCTA CCTGGAGCTG 
GAGGGCCTGC CCTGCATCCT GATCTTCAGT GGGATGGACC CTCACGGGGA ATCCTTACCA 
AGGTCTCTGA GGTACTGTGA CCTGCGTCTG ATAAACTCCT CCTGCTTGGT GAGAACAGCG 
CTGGAGCAGG AGCTGGGCCT GGCCGCTTAC TTTGTGAGCA ACGAGGTTTC TTTGGAGAAG 
GCGGCCCGGA ACGAGCCCTT GGAGAGCGAT GCGGAAAAGC TGAGCAGCAC AGACAACGAG 
GACGAGGAGC TGGTGACGGA AGGCTCCACC TCGGAGAAGA GGAGCCCGGT GAAGCGGGAG 
AGGCCCTGCT CCCATGATTC GGCGGCGTCC TCCCTCTCCT CGAAAGCTTC CGGCTCGGCA 
CCGTGCCGCG AGTCCTCGGC TCAGCCCGGG GCGCCCTCGC AGGGGGAGCG GGCCCGATCA 
CCGCAGCCCT GCGGCCCGTC GGAGGAGGGC AGGGCCCCCG GGGAGAGGCA GAGGCCCCAC 
GCTGGCCGGG GACCGCCGTC GGTCATCAGC AGGCGCAGCC CCGGGCTGGC CGCCCAGCCG 
GACTCCGGCC CCAGGACCAG CCAGAGGAGC GTCCAGGTGT CGGTCACCCC GTCGTCCTCC 
CAGCTGTCCT CCTCCGGCTC GTCGTCCTCG TCCGGGGCGC TCGTCCCGCA GGCCTCCCGG 
TGCTCCCTGA CCAAGGCCTG CCGCCAGCCG CCCGTCGTCT TCCTGCCCAA GCTGGTGTAT 
GACATGGTCA CGGCCACCGA CAGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCC 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGC CCGCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCCC TCTACTACCG GCAGTGGACG GTGCCCCGGC CCAGCCACAT GGACTACAGC 
AACCGCACCG AGGGCCGCGT GGACGGCTTC CACCCGCGCC GGCTGCTGCT CAGCGGGCCC 
CCTCAGGTTG GGAAGACAGG TGCTTACCTG CAGTTCCTTA GTATTTTGTC CAGAATGCTC 
ATCCGGCTGA CGGAAGTGGA TGTTTATGAT GAAGAAGAGA TCAATATCCA CCTCAGAGAG 
GAGTCCGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CAGACCTGGA GCTATTCAAG 
AAGTTGCCTT TTGACTACAT TATTCACGAC CCGAAGTATG AAGACGCCAG TCTGATCTGT 
TCGCACCTTC AGAGCGTGAA GAGTGAAGCC AGGGGGACGT CCCGGAAGCC GGAGGAGCTC 
TACGTGCGGC GCCAGACAGC GCGGATGAGG CTGTCCAAGT ACGCGGCCTA TAACACATAC 
CACCACTGCG AGCAGTGTCA CCACTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTACAT 
TTCATCATCC CCAAGTCCAA AGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GGCTGCCCCT GGTCACAGAC AAGAGCCACG AGTATATAAA AAGTCCGACG 
TTCACCCCGA CAACCGGCCG TCACGAACAC GGACTCTTTA ACCTGTACCA TGCCATGGAT 
GGTGCCAGCC ATCTGCACGT GCTGGTCGTC AAGGAGTATG AAATGGCCAT TTATAAGAAA 
TACTGGCCCA ACCACATCAT GCTGGTGCTC CCAAGTATCT TCAACAGCGC TGGAGTTGGC 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAATC TGGAGCTGGA GAGGAACCGG 
CAGGAGGAGC TGGGGATCAA GCCACAGGAC ATCTGGCCTT TCATCGTGAT CTCGGACGAC 
TCCTGCGTGA TGTGGAACGT GGTGGATGTA GACTGTGGCG GAGAAAGAAG CAGGGAGTTT 
TCCTGGGCAG AAAGAAACGT GTCTTTGAAG CACATCATGC AGCACATCGA GGCGTCCCCC 
AACATCACCC ACTATGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC GGGGAGCGCC 
GAGGTGCGGG AGCCCTTCTC CCGCTGCCAC GTGCACGACT TCATCATTCT GAACGTGGAC 
CTGACCCAGA ACGTGCAGTA CCACCAGAAC CGGTTCACGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ATAGCGCCGG CCTCCTGCTC TGCCGTTTCA ACCGCTTTAG CGTGATGAAG 
AAGCAGATTG CCGTGGGTGG GCACAGGTCC TGCCACATCA CGTCCAAGGT ATCCGACAAC 
CCCGTGGCCA TCGTGCCGGC CCAGTACATC TGCGCCCCGG ACAGCAAGCA CACGTTCCTC 
GCGGCGCCCG CGCAGCTCCT GCTGGAGAAG TTCCTCCAGC ACCACAGTCA CCGATTCTTC 
CCGCTGTCCC TGAGGAACCG CGGCCACCCT GTGCTCTCGG TCGACTGCTA CCTTAACCTG 
GGATCTCAGA TTTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCCCTGAA CATCAGCTGC 
TCTGACTTCG TGTTTAGCGG ACTCCTGCTA TACCTCTGTG ATTCTTTTGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCCTGT GCGTGATCTG CCAGGACCGG 
AACTCCCTGC GCCAGACGGT GGTCCGCCTC GAGCTGGAAG ACGAGTGGCA GTTCCGGCTG 
CGTGACGAAT TCCAGACGGC CAACGCCAAG GAAGACCGGC CGCTCTTTTT TCTGACCGGG 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_027077918.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026933719.1 Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X6, mRNA.

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CloneID	OAc233925
Clone ID Related Accession (Same CDS sequence)	XM_027077916.1 , XM_027077920.1 , XM_027077921.1 , XM_027077917.1 , XM_027077918.1 , XM_027077919.1
Accession Version	XM_027077918.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	protein GREB1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834765.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCCGG CCAGCTGAAG ACGACGCGGT TCGAAGAGGT CTTGCACAAC 
TCCATCGAGG CTTCGCTGCG GTCCAACAGC CTGGTGCCCA GGCCCGTCTT CTCCCAGTTG 
TATCTAGAAG CCGAACAGCA GCTCTCCACG CTAGAAGGTG GAGGCCGAGG GGACAATGAG 
GATGAGGAAG AAGAGGGGGA AGGGGCGCTG GAGCCCAGCG GTCCCCCGAA TCCCTACCAG 
ATGCACCCTC CGCCGGAAGG CTGCTGCACC ACAGACGGGT TCTGCCAGGC GGGGAAGGAC 
CTGCGTCTCG TCTCCATTTC CAGTGAGCCC ATAGACGTCC CTGCAGGCTT TCTCCTCGTG 
GGGGCCAAGT CCCCCAGCCT GCCAGACCAT CTCCTGGTGT GTGCTGTGGA CAAAAGGTTC 
TTGCCGGATG ACAATGGGCA CAACGCTCTT CTTGGTTTCT CTGGGAATTG CGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA CTTCACAGAA TTCTCTAATC ACATAAATCT CAAGTTGACC 
ACTCAGCCCA AGAAGCAGAA ACACTTGAAG TATTACCTGG TCCGCAACGC ACAAGGGACT 
CTAACCAAAG GACCTCTCAT CTGTTGGAAA GGCTCAGAGT TCAGAAGCCG GCCGACCTCC 
GCCAGTGCCT GTTCCAGCTC ACTCTTCCCA CCAGCGGAGA GCTCCGGGCC TCTGGCTGCC 
TTCTCCAGCG AGCCCGTTCC CGGAGCCAGC CCGAGCGTCC CCCTGGGAGC CCAGCAGGCA 
GTGCCAGCCT CTGAGCACCC CTCACTTGCT ACGGCCGTGG GTCCAGCTGT TTTCAATGGC 
AGAGATTCTC CGAAGCACCA ACAGCTGGTG AAGAACGACC TATCGGCCAT GCCACGGCCC 
TCGGCATTAG GTATCTTATC AAACTCTGGG CCCCCGAAGA AACGCCACAA AGGGTGGTCC 
CCTGAATCTC CATCAGCGAC AGACGCCGGC TACGCCCAGG GTGGTGGGAA CAGAGCTAAG 
TACGAGAGCG CAGGCATGTC CTGCGTGCCA CAGGTTGGCT CGGTGGGACC AGCGTCGGTC 
ACCTTCCCCG TTGTGGCCTC TGGAGAACCA GTGTCCGTCC CTGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCGGTGAT ATTTAAAGGC CACGGGAACT TCCCTTACCT CTGCGGGAAC 
CTGAGTGACG TCGTCGTGAG CCCCCTCCTG TACACGTGCT ACCAGAACTC TCAGTCCATC 
TCCCGGGCGT ATGAGCACTA CGGGGCCTCG GCGATACAGC CCATCTCCGA GGAGGTGCAG 
CTCCTACTGA CCGTCTACTA TCTCGTCCAG CTGGCAGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCGTGG CGCGAATCGC ACCTGACCGA GATCCGGCAG 
TACCAGCAGG CGCAGCCGCA GCCCTGCCCG CCCGCGCCCA GCGCCGTGGC CCCCGTCACC 
TCGGCCCAGC TGCCCTGGCT CGCCGGCCTG GCCGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC CCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTCATC 
GAGGGCAAGC TCGCCAAGAC CAACTACGTG GTGATCGTCC GCGCGTGCCG CAGCCCCGCC 
ATCGACTCCT GCGTCGCCGT CACGGGAAAA TACCAAGCCC GAATTCTTTC TGAGAGCCTC 
CTCACCCCAG CGGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACCGG GAAAGTGGAC 
TTGTTGGGGG CGTTTTTCAG CACCCTCTGT CCAGAGGGGG ACATTGACAT TTTGCTGGAC 
AAATTTCATC AGGAAAATCA AGGCCATATT TCGTCGTCAC TTACTGCCTC CTCTGTCACC 
AAATCAGCAT CCCTGGATGC ACCGGCATGC ACAAGTTATA ATCTGGAGCC CCACCACATC 
CGGCCTTTCC AGCTGGCCGT GGCTCAGAAG CTCCTCTCCC ATGTGTGTTC AATTGCGGAT 
TCCAGCACCC AGAATTTGGA CTTAGGATCC TTCGAGAAGG TGGACTTTCT GATTTGTATT 
CCACCCTCAG AAGTGACCTA TCAGCAAACC CTGTTCCACG TCTGGCATTC AGGGGTTTTG 
TCAGAGCTCG GCTTGGAAAA GGAGCACATG ACCAAGCAGA GGGTTGAACA GTATGTTTTG 
AAGCTTGATG CTGAAGCGCA GACAAAATTT AAGTCCTTTC TGCAAAACTC CTTTCAGAAT 
CCGCATACGC TCTTTGTCCT GATCCACGAC CACGCCCACT GGGACCTCGT GAGTAGCGCT 
GTTCATAATC TCTATTCCCA AAGTGACCCG GCTGTGGGAT TGGTGGACCG GTTGCTGAAC 
TGCAGAGAGG TGAAGGAGGC CCCGAACATC GTGACGCTTC ACGTGACTTC CTTCCCTTAC 
GCTCTGCAGA CACAGCACAC CCTCATCAGC CCTTACAATG AGATCCACTG GCCCGCCTCC 
TACAGCAATG GGGTGGACTT ATATCCTGAG AACAAGAAGT ACTTCGGGCT GTCAGAGTTC 
ATTGAGTCCA CCCTTTCGGG ACACAGCCTC CCTTTGCTCA GATATGACAG CTCCTTTGAG 
GCCATGGTCA CGGCATTAGG AAAAAGGTTC CCCCGCCTGC ACAGCGCGGT GATCAGGACC 
TTTGTTCTCG TGCAGCACTA CGCGGCCGCC ATGATGGCCG TGAGCGGCCT GTCGCAGATG 
AAGAACTACA CGTCGGTGGA GACGCTGGAG ATCACCCAGA ACCTCATCAA CTCCCCCAAG 
CAGTGCCCCT GCGGCCACGG GCTCATGGTC CTGCTGCGGG TGCCCTGCTC CCCGCTGGCG 
GCGGTGGCCT ACGAGCGGCT GGCCCACGTG CGGGCACGCC TGGCCCTGGA GGAGCACTTT 
GAGATCATCC TGGGCAACCC CAGCTCCGGC ATCACCGTCG GGAAGCACTT CGTGAAGCAA 
CTCAAGCTGT GGCAGAAGAT CGAGGACGCC GAGTGGAGAC CTCAGACCTA CCTGGAGCTG 
GAGGGCCTGC CCTGCATCCT GATCTTCAGT GGGATGGACC CTCACGGGGA ATCCTTACCA 
AGGTCTCTGA GGTACTGTGA CCTGCGTCTG ATAAACTCCT CCTGCTTGGT GAGAACAGCG 
CTGGAGCAGG AGCTGGGCCT GGCCGCTTAC TTTGTGAGCA ACGAGGTTTC TTTGGAGAAG 
GCGGCCCGGA ACGAGCCCTT GGAGAGCGAT GCGGAAAAGC TGAGCAGCAC AGACAACGAG 
GACGAGGAGC TGGTGACGGA AGGCTCCACC TCGGAGAAGA GGAGCCCGGT GAAGCGGGAG 
AGGCCCTGCT CCCATGATTC GGCGGCGTCC TCCCTCTCCT CGAAAGCTTC CGGCTCGGCA 
CCGTGCCGCG AGTCCTCGGC TCAGCCCGGG GCGCCCTCGC AGGGGGAGCG GGCCCGATCA 
CCGCAGCCCT GCGGCCCGTC GGAGGAGGGC AGGGCCCCCG GGGAGAGGCA GAGGCCCCAC 
GCTGGCCGGG GACCGCCGTC GGTCATCAGC AGGCGCAGCC CCGGGCTGGC CGCCCAGCCG 
GACTCCGGCC CCAGGACCAG CCAGAGGAGC GTCCAGGTGT CGGTCACCCC GTCGTCCTCC 
CAGCTGTCCT CCTCCGGCTC GTCGTCCTCG TCCGGGGCGC TCGTCCCGCA GGCCTCCCGG 
TGCTCCCTGA CCAAGGCCTG CCGCCAGCCG CCCGTCGTCT TCCTGCCCAA GCTGGTGTAT 
GACATGGTCA CGGCCACCGA CAGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCC 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGC CCGCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCCC TCTACTACCG GCAGTGGACG GTGCCCCGGC CCAGCCACAT GGACTACAGC 
AACCGCACCG AGGGCCGCGT GGACGGCTTC CACCCGCGCC GGCTGCTGCT CAGCGGGCCC 
CCTCAGGTTG GGAAGACAGG TGCTTACCTG CAGTTCCTTA GTATTTTGTC CAGAATGCTC 
ATCCGGCTGA CGGAAGTGGA TGTTTATGAT GAAGAAGAGA TCAATATCCA CCTCAGAGAG 
GAGTCCGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CAGACCTGGA GCTATTCAAG 
AAGTTGCCTT TTGACTACAT TATTCACGAC CCGAAGTATG AAGACGCCAG TCTGATCTGT 
TCGCACCTTC AGAGCGTGAA GAGTGAAGCC AGGGGGACGT CCCGGAAGCC GGAGGAGCTC 
TACGTGCGGC GCCAGACAGC GCGGATGAGG CTGTCCAAGT ACGCGGCCTA TAACACATAC 
CACCACTGCG AGCAGTGTCA CCACTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTACAT 
TTCATCATCC CCAAGTCCAA AGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GGCTGCCCCT GGTCACAGAC AAGAGCCACG AGTATATAAA AAGTCCGACG 
TTCACCCCGA CAACCGGCCG TCACGAACAC GGACTCTTTA ACCTGTACCA TGCCATGGAT 
GGTGCCAGCC ATCTGCACGT GCTGGTCGTC AAGGAGTATG AAATGGCCAT TTATAAGAAA 
TACTGGCCCA ACCACATCAT GCTGGTGCTC CCAAGTATCT TCAACAGCGC TGGAGTTGGC 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAATC TGGAGCTGGA GAGGAACCGG 
CAGGAGGAGC TGGGGATCAA GCCACAGGAC ATCTGGCCTT TCATCGTGAT CTCGGACGAC 
TCCTGCGTGA TGTGGAACGT GGTGGATGTA GACTGTGGCG GAGAAAGAAG CAGGGAGTTT 
TCCTGGGCAG AAAGAAACGT GTCTTTGAAG CACATCATGC AGCACATCGA GGCGTCCCCC 
AACATCACCC ACTATGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC GGGGAGCGCC 
GAGGTGCGGG AGCCCTTCTC CCGCTGCCAC GTGCACGACT TCATCATTCT GAACGTGGAC 
CTGACCCAGA ACGTGCAGTA CCACCAGAAC CGGTTCACGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ATAGCGCCGG CCTCCTGCTC TGCCGTTTCA ACCGCTTTAG CGTGATGAAG 
AAGCAGATTG CCGTGGGTGG GCACAGGTCC TGCCACATCA CGTCCAAGGT ATCCGACAAC 
CCCGTGGCCA TCGTGCCGGC CCAGTACATC TGCGCCCCGG ACAGCAAGCA CACGTTCCTC 
GCGGCGCCCG CGCAGCTCCT GCTGGAGAAG TTCCTCCAGC ACCACAGTCA CCGATTCTTC 
CCGCTGTCCC TGAGGAACCG CGGCCACCCT GTGCTCTCGG TCGACTGCTA CCTTAACCTG 
GGATCTCAGA TTTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCCCTGAA CATCAGCTGC 
TCTGACTTCG TGTTTAGCGG ACTCCTGCTA TACCTCTGTG ATTCTTTTGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCCTGT GCGTGATCTG CCAGGACCGG 
AACTCCCTGC GCCAGACGGT GGTCCGCCTC GAGCTGGAAG ACGAGTGGCA GTTCCGGCTG 
CGTGACGAAT TCCAGACGGC CAACGCCAAG GAAGACCGGC CGCTCTTTTT TCTGACCGGG 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026933719.1
CDS	178..6009
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPVFSQLYLEAEQQLSTLEGGGRGDNEDEEEEGEGALEPSGPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGTLTKGPLICWKGSEFRSRPTSASACSSSLFPPAESSGPLAAFSSEPVPGASPSVPLGAQQAVPASEHPSLATAVGPAVFNGRDSPKHQQLVKNDLSAMPRPSALGILSNSGPPKKRHKGWSPESPSATDAGYAQGGGNRAKYESAGMSCVPQVGSVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLSDVVVSPLLYTCYQNSQSISRAYEHYGASAIQPISEEVQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAQPQPCPPAPSAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIVRACRSPAIDSCVAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDLLGAFFSTLCPEGDIDILLDKFHQENQGHISSSLTASSVTKSASLDAPACTSYNLEPHHIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLSELGLEKEHMTKQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPAVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPASYSNGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPSSGITVGKHFVKQLKLWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNEVSLEKAARNEPLESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERPCSHDSAASSLSSKASGSAPCRESSAQPGAPSQGERARSPQPCGPSEEGRAPGERQRPHAGRGPPSVISRRSPGLAAQPDSGPRTSQRSVQVSVTPSSSQLSSSGSSSSSGALVPQASRCSLTKACRQPPVVFLPKLVYDMVTATDSSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPSHMDYSNRTEGRVDGFHPRRLLLSGPPQVGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINIHLREESDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHLQSVKSEARGTSRKPEELYVRRQTARMRLSKYAAYNTYHHCEQCHHYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDCGGERSREFSWAERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSAEVREPFSRCHVHDFIILNVDLTQNVQYHQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSCHITSKVSDNPVAIVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLRNRGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_027077918.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027077918.1

ORF Insert Sequence:

ATGGGAAACT CGTACGCCGG CCAGCTGAAG ACGACGCGGT TCGAAGAGGT CTTGCACAAC 
TCCATCGAGG CTTCGCTGCG GTCCAACAGC CTGGTGCCCA GGCCCGTCTT CTCCCAGTTG 
TATCTAGAAG CCGAACAGCA GCTCTCCACG CTAGAAGGTG GAGGCCGAGG GGACAATGAG 
GATGAGGAAG AAGAGGGGGA AGGGGCGCTG GAGCCCAGCG GTCCCCCGAA TCCCTACCAG 
ATGCACCCTC CGCCGGAAGG CTGCTGCACC ACAGACGGGT TCTGCCAGGC GGGGAAGGAC 
CTGCGTCTCG TCTCCATTTC CAGTGAGCCC ATAGACGTCC CTGCAGGCTT TCTCCTCGTG 
GGGGCCAAGT CCCCCAGCCT GCCAGACCAT CTCCTGGTGT GTGCTGTGGA CAAAAGGTTC 
TTGCCGGATG ACAATGGGCA CAACGCTCTT CTTGGTTTCT CTGGGAATTG CGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA CTTCACAGAA TTCTCTAATC ACATAAATCT CAAGTTGACC 
ACTCAGCCCA AGAAGCAGAA ACACTTGAAG TATTACCTGG TCCGCAACGC ACAAGGGACT 
CTAACCAAAG GACCTCTCAT CTGTTGGAAA GGCTCAGAGT TCAGAAGCCG GCCGACCTCC 
GCCAGTGCCT GTTCCAGCTC ACTCTTCCCA CCAGCGGAGA GCTCCGGGCC TCTGGCTGCC 
TTCTCCAGCG AGCCCGTTCC CGGAGCCAGC CCGAGCGTCC CCCTGGGAGC CCAGCAGGCA 
GTGCCAGCCT CTGAGCACCC CTCACTTGCT ACGGCCGTGG GTCCAGCTGT TTTCAATGGC 
AGAGATTCTC CGAAGCACCA ACAGCTGGTG AAGAACGACC TATCGGCCAT GCCACGGCCC 
TCGGCATTAG GTATCTTATC AAACTCTGGG CCCCCGAAGA AACGCCACAA AGGGTGGTCC 
CCTGAATCTC CATCAGCGAC AGACGCCGGC TACGCCCAGG GTGGTGGGAA CAGAGCTAAG 
TACGAGAGCG CAGGCATGTC CTGCGTGCCA CAGGTTGGCT CGGTGGGACC AGCGTCGGTC 
ACCTTCCCCG TTGTGGCCTC TGGAGAACCA GTGTCCGTCC CTGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCGGTGAT ATTTAAAGGC CACGGGAACT TCCCTTACCT CTGCGGGAAC 
CTGAGTGACG TCGTCGTGAG CCCCCTCCTG TACACGTGCT ACCAGAACTC TCAGTCCATC 
TCCCGGGCGT ATGAGCACTA CGGGGCCTCG GCGATACAGC CCATCTCCGA GGAGGTGCAG 
CTCCTACTGA CCGTCTACTA TCTCGTCCAG CTGGCAGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCGTGG CGCGAATCGC ACCTGACCGA GATCCGGCAG 
TACCAGCAGG CGCAGCCGCA GCCCTGCCCG CCCGCGCCCA GCGCCGTGGC CCCCGTCACC 
TCGGCCCAGC TGCCCTGGCT CGCCGGCCTG GCCGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC CCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTCATC 
GAGGGCAAGC TCGCCAAGAC CAACTACGTG GTGATCGTCC GCGCGTGCCG CAGCCCCGCC 
ATCGACTCCT GCGTCGCCGT CACGGGAAAA TACCAAGCCC GAATTCTTTC TGAGAGCCTC 
CTCACCCCAG CGGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACCGG GAAAGTGGAC 
TTGTTGGGGG CGTTTTTCAG CACCCTCTGT CCAGAGGGGG ACATTGACAT TTTGCTGGAC 
AAATTTCATC AGGAAAATCA AGGCCATATT TCGTCGTCAC TTACTGCCTC CTCTGTCACC 
AAATCAGCAT CCCTGGATGC ACCGGCATGC ACAAGTTATA ATCTGGAGCC CCACCACATC 
CGGCCTTTCC AGCTGGCCGT GGCTCAGAAG CTCCTCTCCC ATGTGTGTTC AATTGCGGAT 
TCCAGCACCC AGAATTTGGA CTTAGGATCC TTCGAGAAGG TGGACTTTCT GATTTGTATT 
CCACCCTCAG AAGTGACCTA TCAGCAAACC CTGTTCCACG TCTGGCATTC AGGGGTTTTG 
TCAGAGCTCG GCTTGGAAAA GGAGCACATG ACCAAGCAGA GGGTTGAACA GTATGTTTTG 
AAGCTTGATG CTGAAGCGCA GACAAAATTT AAGTCCTTTC TGCAAAACTC CTTTCAGAAT 
CCGCATACGC TCTTTGTCCT GATCCACGAC CACGCCCACT GGGACCTCGT GAGTAGCGCT 
GTTCATAATC TCTATTCCCA AAGTGACCCG GCTGTGGGAT TGGTGGACCG GTTGCTGAAC 
TGCAGAGAGG TGAAGGAGGC CCCGAACATC GTGACGCTTC ACGTGACTTC CTTCCCTTAC 
GCTCTGCAGA CACAGCACAC CCTCATCAGC CCTTACAATG AGATCCACTG GCCCGCCTCC 
TACAGCAATG GGGTGGACTT ATATCCTGAG AACAAGAAGT ACTTCGGGCT GTCAGAGTTC 
ATTGAGTCCA CCCTTTCGGG ACACAGCCTC CCTTTGCTCA GATATGACAG CTCCTTTGAG 
GCCATGGTCA CGGCATTAGG AAAAAGGTTC CCCCGCCTGC ACAGCGCGGT GATCAGGACC 
TTTGTTCTCG TGCAGCACTA CGCGGCCGCC ATGATGGCCG TGAGCGGCCT GTCGCAGATG 
AAGAACTACA CGTCGGTGGA GACGCTGGAG ATCACCCAGA ACCTCATCAA CTCCCCCAAG 
CAGTGCCCCT GCGGCCACGG GCTCATGGTC CTGCTGCGGG TGCCCTGCTC CCCGCTGGCG 
GCGGTGGCCT ACGAGCGGCT GGCCCACGTG CGGGCACGCC TGGCCCTGGA GGAGCACTTT 
GAGATCATCC TGGGCAACCC CAGCTCCGGC ATCACCGTCG GGAAGCACTT CGTGAAGCAA 
CTCAAGCTGT GGCAGAAGAT CGAGGACGCC GAGTGGAGAC CTCAGACCTA CCTGGAGCTG 
GAGGGCCTGC CCTGCATCCT GATCTTCAGT GGGATGGACC CTCACGGGGA ATCCTTACCA 
AGGTCTCTGA GGTACTGTGA CCTGCGTCTG ATAAACTCCT CCTGCTTGGT GAGAACAGCG 
CTGGAGCAGG AGCTGGGCCT GGCCGCTTAC TTTGTGAGCA ACGAGGTTTC TTTGGAGAAG 
GCGGCCCGGA ACGAGCCCTT GGAGAGCGAT GCGGAAAAGC TGAGCAGCAC AGACAACGAG 
GACGAGGAGC TGGTGACGGA AGGCTCCACC TCGGAGAAGA GGAGCCCGGT GAAGCGGGAG 
AGGCCCTGCT CCCATGATTC GGCGGCGTCC TCCCTCTCCT CGAAAGCTTC CGGCTCGGCA 
CCGTGCCGCG AGTCCTCGGC TCAGCCCGGG GCGCCCTCGC AGGGGGAGCG GGCCCGATCA 
CCGCAGCCCT GCGGCCCGTC GGAGGAGGGC AGGGCCCCCG GGGAGAGGCA GAGGCCCCAC 
GCTGGCCGGG GACCGCCGTC GGTCATCAGC AGGCGCAGCC CCGGGCTGGC CGCCCAGCCG 
GACTCCGGCC CCAGGACCAG CCAGAGGAGC GTCCAGGTGT CGGTCACCCC GTCGTCCTCC 
CAGCTGTCCT CCTCCGGCTC GTCGTCCTCG TCCGGGGCGC TCGTCCCGCA GGCCTCCCGG 
TGCTCCCTGA CCAAGGCCTG CCGCCAGCCG CCCGTCGTCT TCCTGCCCAA GCTGGTGTAT 
GACATGGTCA CGGCCACCGA CAGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCC 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGC CCGCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCCC TCTACTACCG GCAGTGGACG GTGCCCCGGC CCAGCCACAT GGACTACAGC 
AACCGCACCG AGGGCCGCGT GGACGGCTTC CACCCGCGCC GGCTGCTGCT CAGCGGGCCC 
CCTCAGGTTG GGAAGACAGG TGCTTACCTG CAGTTCCTTA GTATTTTGTC CAGAATGCTC 
ATCCGGCTGA CGGAAGTGGA TGTTTATGAT GAAGAAGAGA TCAATATCCA CCTCAGAGAG 
GAGTCCGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CAGACCTGGA GCTATTCAAG 
AAGTTGCCTT TTGACTACAT TATTCACGAC CCGAAGTATG AAGACGCCAG TCTGATCTGT 
TCGCACCTTC AGAGCGTGAA GAGTGAAGCC AGGGGGACGT CCCGGAAGCC GGAGGAGCTC 
TACGTGCGGC GCCAGACAGC GCGGATGAGG CTGTCCAAGT ACGCGGCCTA TAACACATAC 
CACCACTGCG AGCAGTGTCA CCACTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTACAT 
TTCATCATCC CCAAGTCCAA AGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GGCTGCCCCT GGTCACAGAC AAGAGCCACG AGTATATAAA AAGTCCGACG 
TTCACCCCGA CAACCGGCCG TCACGAACAC GGACTCTTTA ACCTGTACCA TGCCATGGAT 
GGTGCCAGCC ATCTGCACGT GCTGGTCGTC AAGGAGTATG AAATGGCCAT TTATAAGAAA 
TACTGGCCCA ACCACATCAT GCTGGTGCTC CCAAGTATCT TCAACAGCGC TGGAGTTGGC 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAATC TGGAGCTGGA GAGGAACCGG 
CAGGAGGAGC TGGGGATCAA GCCACAGGAC ATCTGGCCTT TCATCGTGAT CTCGGACGAC 
TCCTGCGTGA TGTGGAACGT GGTGGATGTA GACTGTGGCG GAGAAAGAAG CAGGGAGTTT 
TCCTGGGCAG AAAGAAACGT GTCTTTGAAG CACATCATGC AGCACATCGA GGCGTCCCCC 
AACATCACCC ACTATGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC GGGGAGCGCC 
GAGGTGCGGG AGCCCTTCTC CCGCTGCCAC GTGCACGACT TCATCATTCT GAACGTGGAC 
CTGACCCAGA ACGTGCAGTA CCACCAGAAC CGGTTCACGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ATAGCGCCGG CCTCCTGCTC TGCCGTTTCA ACCGCTTTAG CGTGATGAAG 
AAGCAGATTG CCGTGGGTGG GCACAGGTCC TGCCACATCA CGTCCAAGGT ATCCGACAAC 
CCCGTGGCCA TCGTGCCGGC CCAGTACATC TGCGCCCCGG ACAGCAAGCA CACGTTCCTC 
GCGGCGCCCG CGCAGCTCCT GCTGGAGAAG TTCCTCCAGC ACCACAGTCA CCGATTCTTC 
CCGCTGTCCC TGAGGAACCG CGGCCACCCT GTGCTCTCGG TCGACTGCTA CCTTAACCTG 
GGATCTCAGA TTTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCCCTGAA CATCAGCTGC 
TCTGACTTCG TGTTTAGCGG ACTCCTGCTA TACCTCTGTG ATTCTTTTGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCCTGT GCGTGATCTG CCAGGACCGG 
AACTCCCTGC GCCAGACGGT GGTCCGCCTC GAGCTGGAAG ACGAGTGGCA GTTCCGGCTG 
CGTGACGAAT TCCAGACGGC CAACGCCAAG GAAGACCGGC CGCTCTTTTT TCTGACCGGG 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_027077919.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026933720.1 Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X7, mRNA.

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CloneID	OAc233925
Clone ID Related Accession (Same CDS sequence)	XM_027077916.1 , XM_027077920.1 , XM_027077921.1 , XM_027077917.1 , XM_027077918.1 , XM_027077919.1
Accession Version	XM_027077919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	protein GREB1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834765.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAACT CGTACGCCGG CCAGCTGAAG ACGACGCGGT TCGAAGAGGT CTTGCACAAC 
TCCATCGAGG CTTCGCTGCG GTCCAACAGC CTGGTGCCCA GGCCCGTCTT CTCCCAGTTG 
TATCTAGAAG CCGAACAGCA GCTCTCCACG CTAGAAGGTG GAGGCCGAGG GGACAATGAG 
GATGAGGAAG AAGAGGGGGA AGGGGCGCTG GAGCCCAGCG GTCCCCCGAA TCCCTACCAG 
ATGCACCCTC CGCCGGAAGG CTGCTGCACC ACAGACGGGT TCTGCCAGGC GGGGAAGGAC 
CTGCGTCTCG TCTCCATTTC CAGTGAGCCC ATAGACGTCC CTGCAGGCTT TCTCCTCGTG 
GGGGCCAAGT CCCCCAGCCT GCCAGACCAT CTCCTGGTGT GTGCTGTGGA CAAAAGGTTC 
TTGCCGGATG ACAATGGGCA CAACGCTCTT CTTGGTTTCT CTGGGAATTG CGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA CTTCACAGAA TTCTCTAATC ACATAAATCT CAAGTTGACC 
ACTCAGCCCA AGAAGCAGAA ACACTTGAAG TATTACCTGG TCCGCAACGC ACAAGGGACT 
CTAACCAAAG GACCTCTCAT CTGTTGGAAA GGCTCAGAGT TCAGAAGCCG GCCGACCTCC 
GCCAGTGCCT GTTCCAGCTC ACTCTTCCCA CCAGCGGAGA GCTCCGGGCC TCTGGCTGCC 
TTCTCCAGCG AGCCCGTTCC CGGAGCCAGC CCGAGCGTCC CCCTGGGAGC CCAGCAGGCA 
GTGCCAGCCT CTGAGCACCC CTCACTTGCT ACGGCCGTGG GTCCAGCTGT TTTCAATGGC 
AGAGATTCTC CGAAGCACCA ACAGCTGGTG AAGAACGACC TATCGGCCAT GCCACGGCCC 
TCGGCATTAG GTATCTTATC AAACTCTGGG CCCCCGAAGA AACGCCACAA AGGGTGGTCC 
CCTGAATCTC CATCAGCGAC AGACGCCGGC TACGCCCAGG GTGGTGGGAA CAGAGCTAAG 
TACGAGAGCG CAGGCATGTC CTGCGTGCCA CAGGTTGGCT CGGTGGGACC AGCGTCGGTC 
ACCTTCCCCG TTGTGGCCTC TGGAGAACCA GTGTCCGTCC CTGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCGGTGAT ATTTAAAGGC CACGGGAACT TCCCTTACCT CTGCGGGAAC 
CTGAGTGACG TCGTCGTGAG CCCCCTCCTG TACACGTGCT ACCAGAACTC TCAGTCCATC 
TCCCGGGCGT ATGAGCACTA CGGGGCCTCG GCGATACAGC CCATCTCCGA GGAGGTGCAG 
CTCCTACTGA CCGTCTACTA TCTCGTCCAG CTGGCAGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCGTGG CGCGAATCGC ACCTGACCGA GATCCGGCAG 
TACCAGCAGG CGCAGCCGCA GCCCTGCCCG CCCGCGCCCA GCGCCGTGGC CCCCGTCACC 
TCGGCCCAGC TGCCCTGGCT CGCCGGCCTG GCCGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC CCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTCATC 
GAGGGCAAGC TCGCCAAGAC CAACTACGTG GTGATCGTCC GCGCGTGCCG CAGCCCCGCC 
ATCGACTCCT GCGTCGCCGT CACGGGAAAA TACCAAGCCC GAATTCTTTC TGAGAGCCTC 
CTCACCCCAG CGGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACCGG GAAAGTGGAC 
TTGTTGGGGG CGTTTTTCAG CACCCTCTGT CCAGAGGGGG ACATTGACAT TTTGCTGGAC 
AAATTTCATC AGGAAAATCA AGGCCATATT TCGTCGTCAC TTACTGCCTC CTCTGTCACC 
AAATCAGCAT CCCTGGATGC ACCGGCATGC ACAAGTTATA ATCTGGAGCC CCACCACATC 
CGGCCTTTCC AGCTGGCCGT GGCTCAGAAG CTCCTCTCCC ATGTGTGTTC AATTGCGGAT 
TCCAGCACCC AGAATTTGGA CTTAGGATCC TTCGAGAAGG TGGACTTTCT GATTTGTATT 
CCACCCTCAG AAGTGACCTA TCAGCAAACC CTGTTCCACG TCTGGCATTC AGGGGTTTTG 
TCAGAGCTCG GCTTGGAAAA GGAGCACATG ACCAAGCAGA GGGTTGAACA GTATGTTTTG 
AAGCTTGATG CTGAAGCGCA GACAAAATTT AAGTCCTTTC TGCAAAACTC CTTTCAGAAT 
CCGCATACGC TCTTTGTCCT GATCCACGAC CACGCCCACT GGGACCTCGT GAGTAGCGCT 
GTTCATAATC TCTATTCCCA AAGTGACCCG GCTGTGGGAT TGGTGGACCG GTTGCTGAAC 
TGCAGAGAGG TGAAGGAGGC CCCGAACATC GTGACGCTTC ACGTGACTTC CTTCCCTTAC 
GCTCTGCAGA CACAGCACAC CCTCATCAGC CCTTACAATG AGATCCACTG GCCCGCCTCC 
TACAGCAATG GGGTGGACTT ATATCCTGAG AACAAGAAGT ACTTCGGGCT GTCAGAGTTC 
ATTGAGTCCA CCCTTTCGGG ACACAGCCTC CCTTTGCTCA GATATGACAG CTCCTTTGAG 
GCCATGGTCA CGGCATTAGG AAAAAGGTTC CCCCGCCTGC ACAGCGCGGT GATCAGGACC 
TTTGTTCTCG TGCAGCACTA CGCGGCCGCC ATGATGGCCG TGAGCGGCCT GTCGCAGATG 
AAGAACTACA CGTCGGTGGA GACGCTGGAG ATCACCCAGA ACCTCATCAA CTCCCCCAAG 
CAGTGCCCCT GCGGCCACGG GCTCATGGTC CTGCTGCGGG TGCCCTGCTC CCCGCTGGCG 
GCGGTGGCCT ACGAGCGGCT GGCCCACGTG CGGGCACGCC TGGCCCTGGA GGAGCACTTT 
GAGATCATCC TGGGCAACCC CAGCTCCGGC ATCACCGTCG GGAAGCACTT CGTGAAGCAA 
CTCAAGCTGT GGCAGAAGAT CGAGGACGCC GAGTGGAGAC CTCAGACCTA CCTGGAGCTG 
GAGGGCCTGC CCTGCATCCT GATCTTCAGT GGGATGGACC CTCACGGGGA ATCCTTACCA 
AGGTCTCTGA GGTACTGTGA CCTGCGTCTG ATAAACTCCT CCTGCTTGGT GAGAACAGCG 
CTGGAGCAGG AGCTGGGCCT GGCCGCTTAC TTTGTGAGCA ACGAGGTTTC TTTGGAGAAG 
GCGGCCCGGA ACGAGCCCTT GGAGAGCGAT GCGGAAAAGC TGAGCAGCAC AGACAACGAG 
GACGAGGAGC TGGTGACGGA AGGCTCCACC TCGGAGAAGA GGAGCCCGGT GAAGCGGGAG 
AGGCCCTGCT CCCATGATTC GGCGGCGTCC TCCCTCTCCT CGAAAGCTTC CGGCTCGGCA 
CCGTGCCGCG AGTCCTCGGC TCAGCCCGGG GCGCCCTCGC AGGGGGAGCG GGCCCGATCA 
CCGCAGCCCT GCGGCCCGTC GGAGGAGGGC AGGGCCCCCG GGGAGAGGCA GAGGCCCCAC 
GCTGGCCGGG GACCGCCGTC GGTCATCAGC AGGCGCAGCC CCGGGCTGGC CGCCCAGCCG 
GACTCCGGCC CCAGGACCAG CCAGAGGAGC GTCCAGGTGT CGGTCACCCC GTCGTCCTCC 
CAGCTGTCCT CCTCCGGCTC GTCGTCCTCG TCCGGGGCGC TCGTCCCGCA GGCCTCCCGG 
TGCTCCCTGA CCAAGGCCTG CCGCCAGCCG CCCGTCGTCT TCCTGCCCAA GCTGGTGTAT 
GACATGGTCA CGGCCACCGA CAGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCC 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGC CCGCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCCC TCTACTACCG GCAGTGGACG GTGCCCCGGC CCAGCCACAT GGACTACAGC 
AACCGCACCG AGGGCCGCGT GGACGGCTTC CACCCGCGCC GGCTGCTGCT CAGCGGGCCC 
CCTCAGGTTG GGAAGACAGG TGCTTACCTG CAGTTCCTTA GTATTTTGTC CAGAATGCTC 
ATCCGGCTGA CGGAAGTGGA TGTTTATGAT GAAGAAGAGA TCAATATCCA CCTCAGAGAG 
GAGTCCGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CAGACCTGGA GCTATTCAAG 
AAGTTGCCTT TTGACTACAT TATTCACGAC CCGAAGTATG AAGACGCCAG TCTGATCTGT 
TCGCACCTTC AGAGCGTGAA GAGTGAAGCC AGGGGGACGT CCCGGAAGCC GGAGGAGCTC 
TACGTGCGGC GCCAGACAGC GCGGATGAGG CTGTCCAAGT ACGCGGCCTA TAACACATAC 
CACCACTGCG AGCAGTGTCA CCACTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTACAT 
TTCATCATCC CCAAGTCCAA AGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GGCTGCCCCT GGTCACAGAC AAGAGCCACG AGTATATAAA AAGTCCGACG 
TTCACCCCGA CAACCGGCCG TCACGAACAC GGACTCTTTA ACCTGTACCA TGCCATGGAT 
GGTGCCAGCC ATCTGCACGT GCTGGTCGTC AAGGAGTATG AAATGGCCAT TTATAAGAAA 
TACTGGCCCA ACCACATCAT GCTGGTGCTC CCAAGTATCT TCAACAGCGC TGGAGTTGGC 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAATC TGGAGCTGGA GAGGAACCGG 
CAGGAGGAGC TGGGGATCAA GCCACAGGAC ATCTGGCCTT TCATCGTGAT CTCGGACGAC 
TCCTGCGTGA TGTGGAACGT GGTGGATGTA GACTGTGGCG GAGAAAGAAG CAGGGAGTTT 
TCCTGGGCAG AAAGAAACGT GTCTTTGAAG CACATCATGC AGCACATCGA GGCGTCCCCC 
AACATCACCC ACTATGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC GGGGAGCGCC 
GAGGTGCGGG AGCCCTTCTC CCGCTGCCAC GTGCACGACT TCATCATTCT GAACGTGGAC 
CTGACCCAGA ACGTGCAGTA CCACCAGAAC CGGTTCACGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ATAGCGCCGG CCTCCTGCTC TGCCGTTTCA ACCGCTTTAG CGTGATGAAG 
AAGCAGATTG CCGTGGGTGG GCACAGGTCC TGCCACATCA CGTCCAAGGT ATCCGACAAC 
CCCGTGGCCA TCGTGCCGGC CCAGTACATC TGCGCCCCGG ACAGCAAGCA CACGTTCCTC 
GCGGCGCCCG CGCAGCTCCT GCTGGAGAAG TTCCTCCAGC ACCACAGTCA CCGATTCTTC 
CCGCTGTCCC TGAGGAACCG CGGCCACCCT GTGCTCTCGG TCGACTGCTA CCTTAACCTG 
GGATCTCAGA TTTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCCCTGAA CATCAGCTGC 
TCTGACTTCG TGTTTAGCGG ACTCCTGCTA TACCTCTGTG ATTCTTTTGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCCTGT GCGTGATCTG CCAGGACCGG 
AACTCCCTGC GCCAGACGGT GGTCCGCCTC GAGCTGGAAG ACGAGTGGCA GTTCCGGCTG 
CGTGACGAAT TCCAGACGGC CAACGCCAAG GAAGACCGGC CGCTCTTTTT TCTGACCGGG 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026933720.1
CDS	122..5953
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPVFSQLYLEAEQQLSTLEGGGRGDNEDEEEEGEGALEPSGPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGTLTKGPLICWKGSEFRSRPTSASACSSSLFPPAESSGPLAAFSSEPVPGASPSVPLGAQQAVPASEHPSLATAVGPAVFNGRDSPKHQQLVKNDLSAMPRPSALGILSNSGPPKKRHKGWSPESPSATDAGYAQGGGNRAKYESAGMSCVPQVGSVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLSDVVVSPLLYTCYQNSQSISRAYEHYGASAIQPISEEVQLLLTVYYLVQLAADQVPLMEDLEQIFLRSWRESHLTEIRQYQQAQPQPCPPAPSAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIVRACRSPAIDSCVAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDLLGAFFSTLCPEGDIDILLDKFHQENQGHISSSLTASSVTKSASLDAPACTSYNLEPHHIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLSELGLEKEHMTKQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPAVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPASYSNGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRFPRLHSAVIRTFVLVQHYAAAMMAVSGLSQMKNYTSVETLEITQNLINSPKQCPCGHGLMVLLRVPCSPLAAVAYERLAHVRARLALEEHFEIILGNPSSGITVGKHFVKQLKLWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNEVSLEKAARNEPLESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERPCSHDSAASSLSSKASGSAPCRESSAQPGAPSQGERARSPQPCGPSEEGRAPGERQRPHAGRGPPSVISRRSPGLAAQPDSGPRTSQRSVQVSVTPSSSQLSSSGSSSSSGALVPQASRCSLTKACRQPPVVFLPKLVYDMVTATDSSGLPKAASLLPSHSVMWASSFRPLLSKTMTSTEQSLYYRQWTVPRPSHMDYSNRTEGRVDGFHPRRLLLSGPPQVGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINIHLREESDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHLQSVKSEARGTSRKPEELYVRRQTARMRLSKYAAYNTYHHCEQCHHYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDCGGERSREFSWAERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSAEVREPFSRCHVHDFIILNVDLTQNVQYHQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSCHITSKVSDNPVAIVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLRNRGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_027077919.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus growth regulating estrogen receptor binding 1 (GREB1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027077919.1

ORF Insert Sequence:

ATGGGAAACT CGTACGCCGG CCAGCTGAAG ACGACGCGGT TCGAAGAGGT CTTGCACAAC 
TCCATCGAGG CTTCGCTGCG GTCCAACAGC CTGGTGCCCA GGCCCGTCTT CTCCCAGTTG 
TATCTAGAAG CCGAACAGCA GCTCTCCACG CTAGAAGGTG GAGGCCGAGG GGACAATGAG 
GATGAGGAAG AAGAGGGGGA AGGGGCGCTG GAGCCCAGCG GTCCCCCGAA TCCCTACCAG 
ATGCACCCTC CGCCGGAAGG CTGCTGCACC ACAGACGGGT TCTGCCAGGC GGGGAAGGAC 
CTGCGTCTCG TCTCCATTTC CAGTGAGCCC ATAGACGTCC CTGCAGGCTT TCTCCTCGTG 
GGGGCCAAGT CCCCCAGCCT GCCAGACCAT CTCCTGGTGT GTGCTGTGGA CAAAAGGTTC 
TTGCCGGATG ACAATGGGCA CAACGCTCTT CTTGGTTTCT CTGGGAATTG CGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA CTTCACAGAA TTCTCTAATC ACATAAATCT CAAGTTGACC 
ACTCAGCCCA AGAAGCAGAA ACACTTGAAG TATTACCTGG TCCGCAACGC ACAAGGGACT 
CTAACCAAAG GACCTCTCAT CTGTTGGAAA GGCTCAGAGT TCAGAAGCCG GCCGACCTCC 
GCCAGTGCCT GTTCCAGCTC ACTCTTCCCA CCAGCGGAGA GCTCCGGGCC TCTGGCTGCC 
TTCTCCAGCG AGCCCGTTCC CGGAGCCAGC CCGAGCGTCC CCCTGGGAGC CCAGCAGGCA 
GTGCCAGCCT CTGAGCACCC CTCACTTGCT ACGGCCGTGG GTCCAGCTGT TTTCAATGGC 
AGAGATTCTC CGAAGCACCA ACAGCTGGTG AAGAACGACC TATCGGCCAT GCCACGGCCC 
TCGGCATTAG GTATCTTATC AAACTCTGGG CCCCCGAAGA AACGCCACAA AGGGTGGTCC 
CCTGAATCTC CATCAGCGAC AGACGCCGGC TACGCCCAGG GTGGTGGGAA CAGAGCTAAG 
TACGAGAGCG CAGGCATGTC CTGCGTGCCA CAGGTTGGCT CGGTGGGACC AGCGTCGGTC 
ACCTTCCCCG TTGTGGCCTC TGGAGAACCA GTGTCCGTCC CTGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCGGTGAT ATTTAAAGGC CACGGGAACT TCCCTTACCT CTGCGGGAAC 
CTGAGTGACG TCGTCGTGAG CCCCCTCCTG TACACGTGCT ACCAGAACTC TCAGTCCATC 
TCCCGGGCGT ATGAGCACTA CGGGGCCTCG GCGATACAGC CCATCTCCGA GGAGGTGCAG 
CTCCTACTGA CCGTCTACTA TCTCGTCCAG CTGGCAGCGG ACCAGGTGCC CCTGATGGAG 
GACCTGGAGC AGATCTTCCT GCGCTCGTGG CGCGAATCGC ACCTGACCGA GATCCGGCAG 
TACCAGCAGG CGCAGCCGCA GCCCTGCCCG CCCGCGCCCA GCGCCGTGGC CCCCGTCACC 
TCGGCCCAGC TGCCCTGGCT CGCCGGCCTG GCCGCCAGCT CCTGCAACGA CAGCGTGCAC 
ATCATCGAGT GCACCTACTC CCTGGCCGAG GGCCTCTCCG AGATGTTCCG GCTGCTCATC 
GAGGGCAAGC TCGCCAAGAC CAACTACGTG GTGATCGTCC GCGCGTGCCG CAGCCCCGCC 
ATCGACTCCT GCGTCGCCGT CACGGGAAAA TACCAAGCCC GAATTCTTTC TGAGAGCCTC 
CTCACCCCAG CGGAGTACCA GAAAGAAGTC AATTACGAGC TGGTTACCGG GAAAGTGGAC 
TTGTTGGGGG CGTTTTTCAG CACCCTCTGT CCAGAGGGGG ACATTGACAT TTTGCTGGAC 
AAATTTCATC AGGAAAATCA AGGCCATATT TCGTCGTCAC TTACTGCCTC CTCTGTCACC 
AAATCAGCAT CCCTGGATGC ACCGGCATGC ACAAGTTATA ATCTGGAGCC CCACCACATC 
CGGCCTTTCC AGCTGGCCGT GGCTCAGAAG CTCCTCTCCC ATGTGTGTTC AATTGCGGAT 
TCCAGCACCC AGAATTTGGA CTTAGGATCC TTCGAGAAGG TGGACTTTCT GATTTGTATT 
CCACCCTCAG AAGTGACCTA TCAGCAAACC CTGTTCCACG TCTGGCATTC AGGGGTTTTG 
TCAGAGCTCG GCTTGGAAAA GGAGCACATG ACCAAGCAGA GGGTTGAACA GTATGTTTTG 
AAGCTTGATG CTGAAGCGCA GACAAAATTT AAGTCCTTTC TGCAAAACTC CTTTCAGAAT 
CCGCATACGC TCTTTGTCCT GATCCACGAC CACGCCCACT GGGACCTCGT GAGTAGCGCT 
GTTCATAATC TCTATTCCCA AAGTGACCCG GCTGTGGGAT TGGTGGACCG GTTGCTGAAC 
TGCAGAGAGG TGAAGGAGGC CCCGAACATC GTGACGCTTC ACGTGACTTC CTTCCCTTAC 
GCTCTGCAGA CACAGCACAC CCTCATCAGC CCTTACAATG AGATCCACTG GCCCGCCTCC 
TACAGCAATG GGGTGGACTT ATATCCTGAG AACAAGAAGT ACTTCGGGCT GTCAGAGTTC 
ATTGAGTCCA CCCTTTCGGG ACACAGCCTC CCTTTGCTCA GATATGACAG CTCCTTTGAG 
GCCATGGTCA CGGCATTAGG AAAAAGGTTC CCCCGCCTGC ACAGCGCGGT GATCAGGACC 
TTTGTTCTCG TGCAGCACTA CGCGGCCGCC ATGATGGCCG TGAGCGGCCT GTCGCAGATG 
AAGAACTACA CGTCGGTGGA GACGCTGGAG ATCACCCAGA ACCTCATCAA CTCCCCCAAG 
CAGTGCCCCT GCGGCCACGG GCTCATGGTC CTGCTGCGGG TGCCCTGCTC CCCGCTGGCG 
GCGGTGGCCT ACGAGCGGCT GGCCCACGTG CGGGCACGCC TGGCCCTGGA GGAGCACTTT 
GAGATCATCC TGGGCAACCC CAGCTCCGGC ATCACCGTCG GGAAGCACTT CGTGAAGCAA 
CTCAAGCTGT GGCAGAAGAT CGAGGACGCC GAGTGGAGAC CTCAGACCTA CCTGGAGCTG 
GAGGGCCTGC CCTGCATCCT GATCTTCAGT GGGATGGACC CTCACGGGGA ATCCTTACCA 
AGGTCTCTGA GGTACTGTGA CCTGCGTCTG ATAAACTCCT CCTGCTTGGT GAGAACAGCG 
CTGGAGCAGG AGCTGGGCCT GGCCGCTTAC TTTGTGAGCA ACGAGGTTTC TTTGGAGAAG 
GCGGCCCGGA ACGAGCCCTT GGAGAGCGAT GCGGAAAAGC TGAGCAGCAC AGACAACGAG 
GACGAGGAGC TGGTGACGGA AGGCTCCACC TCGGAGAAGA GGAGCCCGGT GAAGCGGGAG 
AGGCCCTGCT CCCATGATTC GGCGGCGTCC TCCCTCTCCT CGAAAGCTTC CGGCTCGGCA 
CCGTGCCGCG AGTCCTCGGC TCAGCCCGGG GCGCCCTCGC AGGGGGAGCG GGCCCGATCA 
CCGCAGCCCT GCGGCCCGTC GGAGGAGGGC AGGGCCCCCG GGGAGAGGCA GAGGCCCCAC 
GCTGGCCGGG GACCGCCGTC GGTCATCAGC AGGCGCAGCC CCGGGCTGGC CGCCCAGCCG 
GACTCCGGCC CCAGGACCAG CCAGAGGAGC GTCCAGGTGT CGGTCACCCC GTCGTCCTCC 
CAGCTGTCCT CCTCCGGCTC GTCGTCCTCG TCCGGGGCGC TCGTCCCGCA GGCCTCCCGG 
TGCTCCCTGA CCAAGGCCTG CCGCCAGCCG CCCGTCGTCT TCCTGCCCAA GCTGGTGTAT 
GACATGGTCA CGGCCACCGA CAGCAGCGGC CTGCCCAAGG CCGCCTCGCT CCTGCCCTCC 
CACTCGGTCA TGTGGGCCAG CTCCTTCCGC CCGCTGCTCA GCAAGACCAT GACGTCCACG 
GAGCAGTCCC TCTACTACCG GCAGTGGACG GTGCCCCGGC CCAGCCACAT GGACTACAGC 
AACCGCACCG AGGGCCGCGT GGACGGCTTC CACCCGCGCC GGCTGCTGCT CAGCGGGCCC 
CCTCAGGTTG GGAAGACAGG TGCTTACCTG CAGTTCCTTA GTATTTTGTC CAGAATGCTC 
ATCCGGCTGA CGGAAGTGGA TGTTTATGAT GAAGAAGAGA TCAATATCCA CCTCAGAGAG 
GAGTCCGATT GGCATTACCT CCAGCTCAGT GACCCCTGGC CAGACCTGGA GCTATTCAAG 
AAGTTGCCTT TTGACTACAT TATTCACGAC CCGAAGTATG AAGACGCCAG TCTGATCTGT 
TCGCACCTTC AGAGCGTGAA GAGTGAAGCC AGGGGGACGT CCCGGAAGCC GGAGGAGCTC 
TACGTGCGGC GCCAGACAGC GCGGATGAGG CTGTCCAAGT ACGCGGCCTA TAACACATAC 
CACCACTGCG AGCAGTGTCA CCACTACATG GGCTTCCACC CCCGCTACCA GCTCTATGAG 
TCCACCCTGC ACGCCTTTGC CTTCTCTTAC TCCATGCTAG GAGAGGAAAT CCAGCTACAT 
TTCATCATCC CCAAGTCCAA AGAGCACCAC TTTGTCTTCA GCCAACCCGG AGGCCAGCTG 
GAGAGCATGC GGCTGCCCCT GGTCACAGAC AAGAGCCACG AGTATATAAA AAGTCCGACG 
TTCACCCCGA CAACCGGCCG TCACGAACAC GGACTCTTTA ACCTGTACCA TGCCATGGAT 
GGTGCCAGCC ATCTGCACGT GCTGGTCGTC AAGGAGTATG AAATGGCCAT TTATAAGAAA 
TACTGGCCCA ACCACATCAT GCTGGTGCTC CCAAGTATCT TCAACAGCGC TGGAGTTGGC 
GCCGCCCACT TCCTGATCAA GGAGCTGTCT TACCACAATC TGGAGCTGGA GAGGAACCGG 
CAGGAGGAGC TGGGGATCAA GCCACAGGAC ATCTGGCCTT TCATCGTGAT CTCGGACGAC 
TCCTGCGTGA TGTGGAACGT GGTGGATGTA GACTGTGGCG GAGAAAGAAG CAGGGAGTTT 
TCCTGGGCAG AAAGAAACGT GTCTTTGAAG CACATCATGC AGCACATCGA GGCGTCCCCC 
AACATCACCC ACTATGCCCT GATTGGCATG CGGAAGTGGT CCAGCAAGAC GGGGAGCGCC 
GAGGTGCGGG AGCCCTTCTC CCGCTGCCAC GTGCACGACT TCATCATTCT GAACGTGGAC 
CTGACCCAGA ACGTGCAGTA CCACCAGAAC CGGTTCACGT GTGACGACGT GGACTTCAAC 
CTGCGGGTGC ATAGCGCCGG CCTCCTGCTC TGCCGTTTCA ACCGCTTTAG CGTGATGAAG 
AAGCAGATTG CCGTGGGTGG GCACAGGTCC TGCCACATCA CGTCCAAGGT ATCCGACAAC 
CCCGTGGCCA TCGTGCCGGC CCAGTACATC TGCGCCCCGG ACAGCAAGCA CACGTTCCTC 
GCGGCGCCCG CGCAGCTCCT GCTGGAGAAG TTCCTCCAGC ACCACAGTCA CCGATTCTTC 
CCGCTGTCCC TGAGGAACCG CGGCCACCCT GTGCTCTCGG TCGACTGCTA CCTTAACCTG 
GGATCTCAGA TTTCTGTGTG TTATGTGAGC TCCAGGCCCC ACTCCCTGAA CATCAGCTGC 
TCTGACTTCG TGTTTAGCGG ACTCCTGCTA TACCTCTGTG ATTCTTTTGT GGGAGCTAGC 
TTTTTGAAAA AGTTTCATTT TCTGAAAGGT GCCACCCTGT GCGTGATCTG CCAGGACCGG 
AACTCCCTGC GCCAGACGGT GGTCCGCCTC GAGCTGGAAG ACGAGTGGCA GTTCCGGCTG 
CGTGACGAAT TCCAGACGGC CAACGCCAAG GAAGACCGGC CGCTCTTTTT TCTGACCGGG 
CGGCACATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1 ( XM_015074747.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_014930233.1 Acinonyx jubatus growth regulation by estrogen in breast cancer 1 (GREB1), mRNA.

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CloneID	OAc47770
Clone ID Related Accession (Same CDS sequence)	XM_015074747.1
Accession Version	XM_015074747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Acinonyx jubatus(cheetah)
Product	protein GREB1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015131077.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acinonyx jubatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGGGAAACT CGTACGCCGG CCAGCTGAAG ACGACGCGGT TCGAAGAGGT CTTGCACAAC 
TCCATCGAGG CTTCGCTGCG GTCCAACAGC CTGGTGCCCA GGCCCGTCTT CTCCCAGTTG 
TATCTAGAAG CCGAACAGCA GCTCTCCACG CTAGAAGGTG GAGGCCGAGG GGACAATGAG 
GATGAGGAAG AAGAGGGGGA AGGGGCGCTG GAGCCCAGCG GTCCCCCGAA TCCCTACCAG 
ATGCACCCTC CGCCGGAAGG CTGCTGCACC ACAGACGGGT TCTGCCAGGC GGGGAAGGAC 
CTGCGTCTCG TCTCCATTTC CAGTGAGCCC ATAGACGTCC CTGCAGGCTT TCTCCTCGTG 
GGGGCCAAGT CCCCCAGCCT GCCAGACCAT CTCCTGGTGT GTGCTGTGGA CAAAAGGTTC 
TTGCCGGATG ACAATGGGCA CAACGCTCTT CTTGGTTTCT CTGGGAATTG CGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA CTTCACAGAA TTCTCTAATC ACATAAATCT CAAGTTGACC 
ACTCAGCCCA AGAAGCAGAA ACACTTGAAG TATTACCTGG TCCGCAACGC ACAAGGGACT 
CTAACCAAAG GACCTCTCAT CTGTTGGAAA GGCTCAGAGT TCAGAAGCCG GCCGACCTCC 
GCCAGTGCCT GTTCCAGCTC ACTCTTCCCA CCAGCGGAGA GCTCCGGGCC TCTGGCTGCC 
TTCTCCAGCG AGCCCGTTCC CGGAGCCAGC CCGAGCGTCC CCCTGGGAGC CCAGCAGGCA 
GTGCCAGCCT CTGAGCACCC CTCACTTGCT ACGGCCGTGG GTCCAGCTGT TTTCAATGGC 
AGAGATTCTC CGAAGCACCA ACAGCTGGTG AAGAACGACC TATCGGCCAT GCCACGGCCC 
TCGGCATTAG GTATCTTATC AAACTCTGGG CCCCCGAAGA AACGCCACAA AGGGTGGTCC 
CCAGAATCTC CATCAGCGAC AGACGCCGGC TACGCCCAGG GTGGTGGGAA CAGAGCTAAG 
TACGAGAGCG CAGGCATGTC CTGCGTGCCA CAGGTTGGCT CGGTGGGACC AGCGTCGGTC 
ACCTTCCCCG TTGTGGCCTC TGGAGAACCA GTGTCCGTCC CTGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCGGTGAT ATTTAAAGGC CACGGGAACT TCCCTTACCT CTGCGGGAAC 
CTGAGTGACG TCGTCGTGAG CCCCCTCCTG TACACGTGCT ACCAGAACTC TCAGTCCATC 
TCCCGGGCGT ATGAGCACTA CGGGGCCTCG GCGATACAGC CCATCTCCGA GGAGGTGCAG 
CTCCTACTGA CCGTCTACTA TCTCGTCCAG CTGGGGTGCG GCTCTGCCCC CCATTCTGCA 
CAGCTGTTAA CGATTGTGAA TCTCATCCCC TGCCGTGCCC TGCGGCAGAC CCGTCTACCC 
CCGAGAAACG CCCCTGCCCA GGACCTGTCC TCCGAGTTTT GGTTCCTGTG TCACCTCAGG 
GAGAACCTTA TCACCATATC TAAGACAGTG CCCCCTCCGC AGCCGCACCC CTGCCCGCCC 
GCGCCCAGCG CCGTGGCCCC CGTCACCTCG GCCCAGCTGC CCTGGCTCGC CGGCCTGGCC 
GCCAGCTCCT GCAACGACAG CGTGCACATC ATCGAGTGCA CCTACTCCCT GGCCGAGGGC 
CTCTCCGAGA TGTTCCGGCT GCTCATCGAG GGCAAGCTCG CCAAGACCAA CTACGTGGTG 
ATCGTCCGCG CGTGCCGCAG CCCCGCCATC GACTCCTGCG TCGCCGTCAC GGGAAAATAC 
CAAGCCCGAA TTCTTTCTGA GAGCCTCCTC ACCCCAGCGG AGTACCAGAA AGAAGTCAAT 
TACGAGCTGG TTACCGGGAA AGTGGACTTG TTGGGGGCGT TTTTCAGCAC CCTCTGTCCA 
GAGGGGGACA TTGACATTTT GCTGGACAAA TTTCATCAGG AAAATCAAGG CCATATTTCG 
TCGTCACTTA CTGCCTCCTC TGTCACCAAA TCAGCATCCC TGGATGCACC GGCATGCACA 
AGTTATAATC TGGAGCCCCA CCACATCCGG CCTTTCCAGC TGGCCGTGGC TCAGAAGCTC 
CTCTCCCATG TGTGTTCAAT TGCGGATTCC AGCACCCAGA ATTTGGACTT AGGATCCTTC 
GAGAAGGTGG ACTTTCTGAT TTGTATTCCA CCCTCAGAAG TGACCTATCA GCAAACCCTG 
TTCCACGTCT GGCATTCAGG GGTTTTGTCA GAGCTCGGCT TGGAAAAGGA GCACATGACC 
AAGCAGAGGG TTGAACAGTA TGTTTTGAAG CTTGATGCTG AAGCGCAGAC AAAATTTAAG 
TCCTTTCTGC AAAACTCCTT TCAGAATCCG CATACGCTCT TTGTCCTGAT CCACGACCAC 
GCCCACTGGG ACCTCGTGAG TAGCGCTGTT CATAATCTCT ATTCCCAAAG TGACCCGGCT 
GTGGGATTGG TGGACCGGTT GCTGAACTGC AGAGAGGTGA AGGAGGCCCC GAACATCGTG 
ACGCTTCACG TGACTTCCTT CCCTTACGCT CTGCAGACAC AGCACACCCT CATCAGCCCT 
TACAATGAGA TCCACTGGCC CGCCTCCTAC AGCAATGGGG TGGACTTATA TCCTGAGAAC 
AAGAAGTACT TCGGGCTGTC AGAGTTCATT GAGTCCACCC TTTCGGGACA CAGCCTCCCT 
TTGCTCAGAT ATGACAGCTC CTTTGAGGCC ATGGTCACGG CATTAGGAAA AAGGTACACG 
TCTCTGTTGG AAGTTGCTAC AAATGCTCAG GATTCCCGTG CCAGTGGAAG CATGTGCAGC 
CAGAGAATTC CAGGGAGGGA GCCGCCTTCC TCCTTTCCTG GGAATCCTGT GTCTGGAAAA 
CTCTCTCGAA ACAGGCAACT GGGACAGAAG GCGATAAAGC TGTGGCAGAA GATCGAGGAC 
GCCGAGTGGA GACCTCAGAC CTACCTGGAG CTGGAGGGCC TGCCCTGCAT CCTGATCTTC 
AGTGGGATGG ACCCTCACGG GGAATCCTTA CCAAGGTCTC TGAGGTACTG TGACCTGCGT 
CTGATAAACT CCTCCTGCTT GGTGAGAACA GCGCTGGAGC AGGAGCTGGG CCTGGCCGCT 
TACTTTGTGA GCAACGAGGT TTCTTTGGAG AAGGCGGCCC GGAACGAGCC CTTGGAGAGC 
GATGCGGAAA AGCTGAGCAG CACAGACAAC GAGGACGAGG AGCTGGTGAC GGAAGGCTCC 
ACCTCGGAGA AGAGGAGCCC GGTGAAGCGG GAGAGGCCCT GCTCCCATGA TTCGGCGGCG 
TCCTCCCTCT CCTCGAAAGC TTCCGGCTCG GCACCGTGCC GCGAGTCCTC GGCTCAGCCC 
GGGGCGCCCT CGCAGGGGGT TGGGAAGACA GGTGCTTACC TGCAGTTCCT TAGTATTTTG 
TCCAGAATGC TCATCCGGCT GACGGAAGTG GATGTTTATG ATGAAGAAGA GATCAATATC 
CACCTCAGAG AGGAGTCCGA TTGGCATTAC CTCCAGCTCA GTGACCCCTG GCCAGACCTG 
GAGCTATTCA AGAAGTTGCC TTTTGACTAC ATTATTCACG ACCCGAAGTA TGAAGACGCC 
AGTCTGATCT GTTCGCACCT TCAGAGCGTG AAGAGTGAAG CCAGGGGGAC GTCCCGGAAG 
CCGGAGGAGC TCTACGTGCG GCGCCAGACA GCGCGGATGA GGCTGTCCAA GTACGCGGCC 
TATAACACAT ACCACCACTG CGAGCAGTGT CACCACTACA TGGGCTTCCA CCCCCGCTAC 
CAGCTCTATG AGTCCACCCT GCACGCCTTT GCCTTCTCTT ACTCCATGCT AGGAGAGGAA 
ATCCAGCTAC ATTTCATCAT CCCCAAGTCC AAAGAGCACC ACTTTGTCTT CAGCCAACCC 
GGAGGCCAGC TGGAGAGCAT GCGGCTGCCC CTGGTCACAG ACAAGAGCCA CGAGTATATA 
AAAAGTCCGA CGTTCACCCC GACAACCGGC CGTCACGAAC ACGGACTCTT TAACCTGTAC 
CATGCCATGG ATGGTGCCAG CCATCTGCAC GTGCTGGTCG TCAAGGAGTA TGAAATGGCC 
ATTTATAAGA AATACTGGCC CAACCACATC ATGCTGGTGC TCCCAAGTAT CTTCAACAGC 
GCTGGAGTTG GCGCCGCCCA CTTCCTGATC AAGGAGCTGT CTTACCACAA TCTGGAGCTG 
GAGAGGAACC GGCAGGAGGA GCTGGGGATC AAGCCACAGG ACATCTGGCC TTTCATCGTG 
ATCTCGGACG ACTCCTGCGT GATGTGGAAC GTGGTGGATG TAGACTGTGG CGGAGAAAGA 
AGCAGGGAGT TTTCCTGGGC AGAAAGAAAC GTGTCTTTGA AGCACATCAT GCAGCACATC 
GAGGCGTCCC CCAACATCAC CCACTATGCC CTGATTGGCA TGCGGAAGTG GTCCAGCAAG 
ACGGGGAGCG CCGAGGTGCG GGAGCCCTTC TCCCGCTGCC ACGTGCACGA CTTCATCATT 
CTGAACGTGG ACCTGACCCA GAACGTGCAG TACCACCAGA ACCGGTTCAC GTGTGACGAC 
GTGGACTTCA ACCTGCGGGT GCATAGCGCC GGCCTCCTGC TCTGCCGTTT CAACCGCTTT 
AGCGTGATGA AGAAGCAGAT TGCCGTGGGT GGGCACAGGT CCTGCCACAT CACGTCCAAG 
GTATCCGACA ACCCCGTGGC CATCGTGCCG GCCCAGTACA TCTGCGCCCC GGACAGCAAG 
CACACGTTCC TCGCGGCGCC CGCGCAGCTC CTGCTGGAGA AGTTCCTCCA GCACCACAGT 
CACCGATTCT TCCCGCTGTC CCTGAGGAAC CGCGGCCACC CTGTGCTCTC GGTCGACTGC 
TACCTTAACC TGGGATCTCA GATTTCTGTG TGTTATGTGA GCTCCAGGCC CCACTCCCTG 
AACATCAGCT GCTCTGACTT CGTGTTTAGC GGACTCCTGC TATACCTCTG TGATTCTTTT 
GTGGGAGCTA GCTTTTTGAA AAAGTTTCAT TTTCTGAAAG GTGCCACCCT GTGCGTGATC 
TGCCAGGACC GGAACTCCCT GCGCCAGACG GTGGTCCGCC TCGAGCTGGA AGACGAGTGG 
CAGTTCCGGC TGCGTGACGA ATTCCAGACG GCCAACGCCA AGGAAGACCG GCCGCTCTTT 
TTTCTGACCG GGCGGCACAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014930233.1
CDS	69..5252
Translation	MGNSYAGQLKTTRFEEVLHNSIEASLRSNSLVPRPVFSQLYLEAEQQLSTLEGGGRGDNEDEEEEGEGALEPSGPPNPYQMHPPPEGCCTTDGFCQAGKDLRLVSISSEPIDVPAGFLLVGAKSPSLPDHLLVCAVDKRFLPDDNGHNALLGFSGNCVGCGKKGFCYFTEFSNHINLKLTTQPKKQKHLKYYLVRNAQGTLTKGPLICWKGSEFRSRPTSASACSSSLFPPAESSGPLAAFSSEPVPGASPSVPLGAQQAVPASEHPSLATAVGPAVFNGRDSPKHQQLVKNDLSAMPRPSALGILSNSGPPKKRHKGWSPESPSATDAGYAQGGGNRAKYESAGMSCVPQVGSVGPASVTFPVVASGEPVSVPDNLLKICKAKPVIFKGHGNFPYLCGNLSDVVVSPLLYTCYQNSQSISRAYEHYGASAIQPISEEVQLLLTVYYLVQLGCGSAPHSAQLLTIVNLIPCRALRQTRLPPRNAPAQDLSSEFWFLCHLRENLITISKTVPPPQPHPCPPAPSAVAPVTSAQLPWLAGLAASSCNDSVHIIECTYSLAEGLSEMFRLLIEGKLAKTNYVVIVRACRSPAIDSCVAVTGKYQARILSESLLTPAEYQKEVNYELVTGKVDLLGAFFSTLCPEGDIDILLDKFHQENQGHISSSLTASSVTKSASLDAPACTSYNLEPHHIRPFQLAVAQKLLSHVCSIADSSTQNLDLGSFEKVDFLICIPPSEVTYQQTLFHVWHSGVLSELGLEKEHMTKQRVEQYVLKLDAEAQTKFKSFLQNSFQNPHTLFVLIHDHAHWDLVSSAVHNLYSQSDPAVGLVDRLLNCREVKEAPNIVTLHVTSFPYALQTQHTLISPYNEIHWPASYSNGVDLYPENKKYFGLSEFIESTLSGHSLPLLRYDSSFEAMVTALGKRYTSLLEVATNAQDSRASGSMCSQRIPGREPPSSFPGNPVSGKLSRNRQLGQKAIKLWQKIEDAEWRPQTYLELEGLPCILIFSGMDPHGESLPRSLRYCDLRLINSSCLVRTALEQELGLAAYFVSNEVSLEKAARNEPLESDAEKLSSTDNEDEELVTEGSTSEKRSPVKRERPCSHDSAASSLSSKASGSAPCRESSAQPGAPSQGVGKTGAYLQFLSILSRMLIRLTEVDVYDEEEINIHLREESDWHYLQLSDPWPDLELFKKLPFDYIIHDPKYEDASLICSHLQSVKSEARGTSRKPEELYVRRQTARMRLSKYAAYNTYHHCEQCHHYMGFHPRYQLYESTLHAFAFSYSMLGEEIQLHFIIPKSKEHHFVFSQPGGQLESMRLPLVTDKSHEYIKSPTFTPTTGRHEHGLFNLYHAMDGASHLHVLVVKEYEMAIYKKYWPNHIMLVLPSIFNSAGVGAAHFLIKELSYHNLELERNRQEELGIKPQDIWPFIVISDDSCVMWNVVDVDCGGERSREFSWAERNVSLKHIMQHIEASPNITHYALIGMRKWSSKTGSAEVREPFSRCHVHDFIILNVDLTQNVQYHQNRFTCDDVDFNLRVHSAGLLLCRFNRFSVMKKQIAVGGHRSCHITSKVSDNPVAIVPAQYICAPDSKHTFLAAPAQLLLEKFLQHHSHRFFPLSLRNRGHPVLSVDCYLNLGSQISVCYVSSRPHSLNISCSDFVFSGLLLYLCDSFVGASFLKKFHFLKGATLCVICQDRNSLRQTVVRLELEDEWQFRLRDEFQTANAKEDRPLFFLTGRHI

Target ORF information:

RefSeq Version	XM_015074747.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus growth regulation by estrogen in breast cancer 1 (GREB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015074747.1

ORF Insert Sequence:

ATGGGAAACT CGTACGCCGG CCAGCTGAAG ACGACGCGGT TCGAAGAGGT CTTGCACAAC 
TCCATCGAGG CTTCGCTGCG GTCCAACAGC CTGGTGCCCA GGCCCGTCTT CTCCCAGTTG 
TATCTAGAAG CCGAACAGCA GCTCTCCACG CTAGAAGGTG GAGGCCGAGG GGACAATGAG 
GATGAGGAAG AAGAGGGGGA AGGGGCGCTG GAGCCCAGCG GTCCCCCGAA TCCCTACCAG 
ATGCACCCTC CGCCGGAAGG CTGCTGCACC ACAGACGGGT TCTGCCAGGC GGGGAAGGAC 
CTGCGTCTCG TCTCCATTTC CAGTGAGCCC ATAGACGTCC CTGCAGGCTT TCTCCTCGTG 
GGGGCCAAGT CCCCCAGCCT GCCAGACCAT CTCCTGGTGT GTGCTGTGGA CAAAAGGTTC 
TTGCCGGATG ACAATGGGCA CAACGCTCTT CTTGGTTTCT CTGGGAATTG CGTTGGCTGT 
GGAAAGAAAG GCTTCTGCTA CTTCACAGAA TTCTCTAATC ACATAAATCT CAAGTTGACC 
ACTCAGCCCA AGAAGCAGAA ACACTTGAAG TATTACCTGG TCCGCAACGC ACAAGGGACT 
CTAACCAAAG GACCTCTCAT CTGTTGGAAA GGCTCAGAGT TCAGAAGCCG GCCGACCTCC 
GCCAGTGCCT GTTCCAGCTC ACTCTTCCCA CCAGCGGAGA GCTCCGGGCC TCTGGCTGCC 
TTCTCCAGCG AGCCCGTTCC CGGAGCCAGC CCGAGCGTCC CCCTGGGAGC CCAGCAGGCA 
GTGCCAGCCT CTGAGCACCC CTCACTTGCT ACGGCCGTGG GTCCAGCTGT TTTCAATGGC 
AGAGATTCTC CGAAGCACCA ACAGCTGGTG AAGAACGACC TATCGGCCAT GCCACGGCCC 
TCGGCATTAG GTATCTTATC AAACTCTGGG CCCCCGAAGA AACGCCACAA AGGGTGGTCC 
CCAGAATCTC CATCAGCGAC AGACGCCGGC TACGCCCAGG GTGGTGGGAA CAGAGCTAAG 
TACGAGAGCG CAGGCATGTC CTGCGTGCCA CAGGTTGGCT CGGTGGGACC AGCGTCGGTC 
ACCTTCCCCG TTGTGGCCTC TGGAGAACCA GTGTCCGTCC CTGACAACTT GCTGAAAATA 
TGCAAGGCCA AGCCGGTGAT ATTTAAAGGC CACGGGAACT TCCCTTACCT CTGCGGGAAC 
CTGAGTGACG TCGTCGTGAG CCCCCTCCTG TACACGTGCT ACCAGAACTC TCAGTCCATC 
TCCCGGGCGT ATGAGCACTA CGGGGCCTCG GCGATACAGC CCATCTCCGA GGAGGTGCAG 
CTCCTACTGA CCGTCTACTA TCTCGTCCAG CTGGGGTGCG GCTCTGCCCC CCATTCTGCA 
CAGCTGTTAA CGATTGTGAA TCTCATCCCC TGCCGTGCCC TGCGGCAGAC CCGTCTACCC 
CCGAGAAACG CCCCTGCCCA GGACCTGTCC TCCGAGTTTT GGTTCCTGTG TCACCTCAGG 
GAGAACCTTA TCACCATATC TAAGACAGTG CCCCCTCCGC AGCCGCACCC CTGCCCGCCC 
GCGCCCAGCG CCGTGGCCCC CGTCACCTCG GCCCAGCTGC CCTGGCTCGC CGGCCTGGCC 
GCCAGCTCCT GCAACGACAG CGTGCACATC ATCGAGTGCA CCTACTCCCT GGCCGAGGGC 
CTCTCCGAGA TGTTCCGGCT GCTCATCGAG GGCAAGCTCG CCAAGACCAA CTACGTGGTG 
ATCGTCCGCG CGTGCCGCAG CCCCGCCATC GACTCCTGCG TCGCCGTCAC GGGAAAATAC 
CAAGCCCGAA TTCTTTCTGA GAGCCTCCTC ACCCCAGCGG AGTACCAGAA AGAAGTCAAT 
TACGAGCTGG TTACCGGGAA AGTGGACTTG TTGGGGGCGT TTTTCAGCAC CCTCTGTCCA 
GAGGGGGACA TTGACATTTT GCTGGACAAA TTTCATCAGG AAAATCAAGG CCATATTTCG 
TCGTCACTTA CTGCCTCCTC TGTCACCAAA TCAGCATCCC TGGATGCACC GGCATGCACA 
AGTTATAATC TGGAGCCCCA CCACATCCGG CCTTTCCAGC TGGCCGTGGC TCAGAAGCTC 
CTCTCCCATG TGTGTTCAAT TGCGGATTCC AGCACCCAGA ATTTGGACTT AGGATCCTTC 
GAGAAGGTGG ACTTTCTGAT TTGTATTCCA CCCTCAGAAG TGACCTATCA GCAAACCCTG 
TTCCACGTCT GGCATTCAGG GGTTTTGTCA GAGCTCGGCT TGGAAAAGGA GCACATGACC 
AAGCAGAGGG TTGAACAGTA TGTTTTGAAG CTTGATGCTG AAGCGCAGAC AAAATTTAAG 
TCCTTTCTGC AAAACTCCTT TCAGAATCCG CATACGCTCT TTGTCCTGAT CCACGACCAC 
GCCCACTGGG ACCTCGTGAG TAGCGCTGTT CATAATCTCT ATTCCCAAAG TGACCCGGCT 
GTGGGATTGG TGGACCGGTT GCTGAACTGC AGAGAGGTGA AGGAGGCCCC GAACATCGTG 
ACGCTTCACG TGACTTCCTT CCCTTACGCT CTGCAGACAC AGCACACCCT CATCAGCCCT 
TACAATGAGA TCCACTGGCC CGCCTCCTAC AGCAATGGGG TGGACTTATA TCCTGAGAAC 
AAGAAGTACT TCGGGCTGTC AGAGTTCATT GAGTCCACCC TTTCGGGACA CAGCCTCCCT 
TTGCTCAGAT ATGACAGCTC CTTTGAGGCC ATGGTCACGG CATTAGGAAA AAGGTACACG 
TCTCTGTTGG AAGTTGCTAC AAATGCTCAG GATTCCCGTG CCAGTGGAAG CATGTGCAGC 
CAGAGAATTC CAGGGAGGGA GCCGCCTTCC TCCTTTCCTG GGAATCCTGT GTCTGGAAAA 
CTCTCTCGAA ACAGGCAACT GGGACAGAAG GCGATAAAGC TGTGGCAGAA GATCGAGGAC 
GCCGAGTGGA GACCTCAGAC CTACCTGGAG CTGGAGGGCC TGCCCTGCAT CCTGATCTTC 
AGTGGGATGG ACCCTCACGG GGAATCCTTA CCAAGGTCTC TGAGGTACTG TGACCTGCGT 
CTGATAAACT CCTCCTGCTT GGTGAGAACA GCGCTGGAGC AGGAGCTGGG CCTGGCCGCT 
TACTTTGTGA GCAACGAGGT TTCTTTGGAG AAGGCGGCCC GGAACGAGCC CTTGGAGAGC 
GATGCGGAAA AGCTGAGCAG CACAGACAAC GAGGACGAGG AGCTGGTGAC GGAAGGCTCC 
ACCTCGGAGA AGAGGAGCCC GGTGAAGCGG GAGAGGCCCT GCTCCCATGA TTCGGCGGCG 
TCCTCCCTCT CCTCGAAAGC TTCCGGCTCG GCACCGTGCC GCGAGTCCTC GGCTCAGCCC 
GGGGCGCCCT CGCAGGGGGT TGGGAAGACA GGTGCTTACC TGCAGTTCCT TAGTATTTTG 
TCCAGAATGC TCATCCGGCT GACGGAAGTG GATGTTTATG ATGAAGAAGA GATCAATATC 
CACCTCAGAG AGGAGTCCGA TTGGCATTAC CTCCAGCTCA GTGACCCCTG GCCAGACCTG 
GAGCTATTCA AGAAGTTGCC TTTTGACTAC ATTATTCACG ACCCGAAGTA TGAAGACGCC 
AGTCTGATCT GTTCGCACCT TCAGAGCGTG AAGAGTGAAG CCAGGGGGAC GTCCCGGAAG 
CCGGAGGAGC TCTACGTGCG GCGCCAGACA GCGCGGATGA GGCTGTCCAA GTACGCGGCC 
TATAACACAT ACCACCACTG CGAGCAGTGT CACCACTACA TGGGCTTCCA CCCCCGCTAC 
CAGCTCTATG AGTCCACCCT GCACGCCTTT GCCTTCTCTT ACTCCATGCT AGGAGAGGAA 
ATCCAGCTAC ATTTCATCAT CCCCAAGTCC AAAGAGCACC ACTTTGTCTT CAGCCAACCC 
GGAGGCCAGC TGGAGAGCAT GCGGCTGCCC CTGGTCACAG ACAAGAGCCA CGAGTATATA 
AAAAGTCCGA CGTTCACCCC GACAACCGGC CGTCACGAAC ACGGACTCTT TAACCTGTAC 
CATGCCATGG ATGGTGCCAG CCATCTGCAC GTGCTGGTCG TCAAGGAGTA TGAAATGGCC 
ATTTATAAGA AATACTGGCC CAACCACATC ATGCTGGTGC TCCCAAGTAT CTTCAACAGC 
GCTGGAGTTG GCGCCGCCCA CTTCCTGATC AAGGAGCTGT CTTACCACAA TCTGGAGCTG 
GAGAGGAACC GGCAGGAGGA GCTGGGGATC AAGCCACAGG ACATCTGGCC TTTCATCGTG 
ATCTCGGACG ACTCCTGCGT GATGTGGAAC GTGGTGGATG TAGACTGTGG CGGAGAAAGA 
AGCAGGGAGT TTTCCTGGGC AGAAAGAAAC GTGTCTTTGA AGCACATCAT GCAGCACATC 
GAGGCGTCCC CCAACATCAC CCACTATGCC CTGATTGGCA TGCGGAAGTG GTCCAGCAAG 
ACGGGGAGCG CCGAGGTGCG GGAGCCCTTC TCCCGCTGCC ACGTGCACGA CTTCATCATT 
CTGAACGTGG ACCTGACCCA GAACGTGCAG TACCACCAGA ACCGGTTCAC GTGTGACGAC 
GTGGACTTCA ACCTGCGGGT GCATAGCGCC GGCCTCCTGC TCTGCCGTTT CAACCGCTTT 
AGCGTGATGA AGAAGCAGAT TGCCGTGGGT GGGCACAGGT CCTGCCACAT CACGTCCAAG 
GTATCCGACA ACCCCGTGGC CATCGTGCCG GCCCAGTACA TCTGCGCCCC GGACAGCAAG 
CACACGTTCC TCGCGGCGCC CGCGCAGCTC CTGCTGGAGA AGTTCCTCCA GCACCACAGT 
CACCGATTCT TCCCGCTGTC CCTGAGGAAC CGCGGCCACC CTGTGCTCTC GGTCGACTGC 
TACCTTAACC TGGGATCTCA GATTTCTGTG TGTTATGTGA GCTCCAGGCC CCACTCCCTG 
AACATCAGCT GCTCTGACTT CGTGTTTAGC GGACTCCTGC TATACCTCTG TGATTCTTTT 
GTGGGAGCTA GCTTTTTGAA AAAGTTTCAT TTTCTGAAAG GTGCCACCCT GTGCGTGATC 
TGCCAGGACC GGAACTCCCT GCGCCAGACG GTGGTCCGCC TCGAGCTGGA AGACGAGTGG 
CAGTTCCGGC TGCGTGACGA ATTCCAGACG GCCAACGCCA AGGAAGACCG GCCGCTCTTT 
TTTCTGACCG GGCGGCACAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GREB1
Entrez Gene ID	106977264
Full Name	growth regulation by estrogen in breast cancer 1
Gene Type	protein-coding
Organism	Acinonyx jubatus(cheetah)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

GREB1 cDNA ORF clone, Acinonyx jubatus(cheetah)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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