ATP2A2 cDNA ORF clone, Acinonyx jubatus(cheetah)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP2A2
Entrez Gene ID	106967538
Full Name	ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
Gene Type	protein-coding
Organism	Acinonyx jubatus(cheetah)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015063763.1	XP_014919249.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 2
XM_027044207.1	XP_026900008.1	sarcoplasmic/endoplasmic reticulum calcium ATPase 2

Pathways from BioSystems
Gene Ontology

The following ATP2A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP2A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc242454	XM_027044207.1 Latest version!	Acinonyx jubatus ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (ATP2A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAc39411	XM_015063763.1 Latest version!	Acinonyx jubatus ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (ATP2A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP2A2 ( XM_027044207.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026900008.1 Acinonyx jubatus ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (ATP2A2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc242454
Clone ID Related Accession (Same CDS sequence)	XM_027044207.1
Accession Version	XM_027044207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834793.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG CGCACACAAA GACGGTGGAG GAGGTGCTGG GCTACTTCGG CGTCAACGAG 
AGCACAGGGC TAAGCCTGGA GCAGGTCAAG AAGCTCAAGG AGAGATGGGG CTCCAATGAG 
TTACCGGCTG AAGAAGGAAA AACCTTGTTG GAACTTGTGA TTGAGCAGTT TGAAGACTTA 
TTAGTTAGAA TTTTATTGCT GGCAGCTTGT ATATCTTTTG TTTTGGCTTG GTTTGAAGAA 
GGTGAAGAAA CAATTACAGC CTTTGTAGAA CCCTTTGTAA TTTTACTTAT ATTAGTAGCC 
AATGCTATTG TGGGTGTGTG GCAGGAAAGA AATGCTGAGA ATGCTATTGA AGCCCTTAAG 
GAATATGAGC CTGAAATGGG CAAAGTATAT CGACAGGACA GAAAGAGTGT ACAGCGGATT 
AAAGCTAAGG ACATAGTTCC TGGTGATATT GTAGAAATTG CTGTTGGTGA CAAAGTTCCT 
GCTGATATAA GATTAACTTC CATCAAATCT ACTACTCTGA GAGTTGACCA GTCCATTCTC 
ACAGGTGAGT CTGTGTCTGT CATCAAGCAC ACTGACCCTG TCCCAGACCC ACGGGCCGTC 
AACCAAGATA AGAAGAACAT GCTGTTTTCT GGAACAAACA TTGCTGCTGG CAAAGCCATG 
GGAGTGGTGG TGGCAACTGG AGTTAACACA GAAATCGGCA AGATCCGGGA TGAGATGGTG 
GCAACAGAAC AGGAGAGGAC ACCCCTCCAG CAGAAACTAG ACGAGTTTGG GGAGCAGCTT 
TCCAAAGTCA TATCCCTTAT TTGCATTGCA GTCTGGATCA TAAACATTGG GCACTTCAAT 
GACCCAGTTC ACGGAGGCTC CTGGATCAGG GGTGCTATTT ACTACTTTAA AATTGCGGTG 
GCCCTGGCTG TGGCGGCCAT TCCTGAAGGT CTGCCTGCTG TCATCACCAC CTGCCTGGCT 
CTTGGAACTC GCAGAATGGC AAAGAAAAAT GCCATTGTTC GAAGTCTCCC TTCTGTGGAA 
ACCCTTGGTT GTACTTCTGT TATCTGCTCA GACAAGACTG GTACGCTTAC GACGAACCAG 
ATGTCAGTGT GCAGGATGTT CATTCTGGAC AAGGTTGAGG GGGATACTTG TTCCCTTAAT 
GAGTTTACCA TAACTGGGTC AACATATGCA CCTATTGGAG AAGTGCATAA AGATGATAAA 
CCAGTGAAAT GTCATCAATA TGATGGTCTT GTGGAATTGG CAACAATTTG TGCTCTTTGT 
AATGATTCTG CATTGGATTA CAATGAGGCA AAGGGCGTGT ATGAAAAAGT TGGGGAAGCT 
ACAGAGACTG CTCTCACTTG CCTAGTAGAG AAGATGAATG TATTTGATAC CGAATTGAAG 
GGTCTTTCTA AAATAGAAAG AGCAAATGCC TGCAACTCGG TCATAAAGCA GTTAATGAAA 
AAAGAATTTA CTCTCGAGTT TTCACGCGAT AGAAAATCAA TGTCAGTTTA TTGTACACCA 
AATAAACCAA GCCGGACATC GATGAGCAAA ATGTTTGTGA AGGGCGCTCC CGAAGGTGTC 
ATTGACAGGT GCACCCACAT TCGGGTTGGA AGTACCAAAG TCCCTATGAC CCCTGGAGTC 
AAACAGAAGG TCATGTCTGT CATCAGGGAA TGGGGTAGTG GCAGCGACAC ACTGCGGTGC 
CTGGCTCTGG CCACTCATGA CAACCCACTG AGAAGAGAAG AAATGAACCT TGAGGACTCT 
GCCAACTTTA TCAAATATGA GACTAATCTG ACTTTCGTTG GCTGTGTGGG CATGCTGGAC 
CCTCCAAGAA TTGAAGTGGC CTCTTCTGTG AAGCTTTGCC GGCAAGCAGG CATCCGGGTG 
ATCATGATCA CAGGGGACAA CAAAGGCACT GCTGTGGCCA TCTGTCGCCG CATTGGCATC 
TTTGGGCAGG ACGAGGATGT GACGTCAAAG GCTTTTACGG GTCGGGAGTT TGATGAGCTC 
AGTCCTTCAG CCCAGAGAGA TGCCTGCTTG AATGCCCGCT GTTTTGCTCG AGTTGAGCCT 
TCCCACAAGT CAAAAATTGT AGAGTTTCTT CAGTCTTTTG ATGAGATTAC AGCTATGACC 
GGGGATGGCG TGAATGATGC TCCTGCCCTG AAGAAATCGG AGATTGGCAT TGCCATGGGC 
TCTGGCACGG CAGTGGCTAA AACCGCCTCT GAGATGGTCC TGGCCGACGA CAACTTCTCC 
ACCATTGTGG CTGCTGTCGA GGAGGGGCGG GCGATTTACA ACAATATGAA GCAGTTTATC 
CGCTACCTCA TCTCTTCGAA TGTGGGGGAA GTTGTCTGTA TTTTCCTGAC TGCAGCCCTT 
GGATTTCCTG AGGCTTTAAT TCCTGTCCAG CTGCTCTGGG TCAATCTGGT GACAGACGGC 
CTGCCTGCCA CCGCACTGGG GTTTAACCCA CCTGATCTGG ACATCATGAA CAAACCACCC 
CGGAACCCGA AGGAACCACT GATCAGCGGA TGGCTCTTTT TCCGTTACCT AGCTATTGGC 
TGTTACGTTG GCGCTGCTAC TGTGGGTGCT GCCGCGTGGT GGTTTATTGC TGCCGACGGG 
GGTCCAAGGG TGTCCTTCTA CCAACTGAGC CACTTCCTAC AGTGTAAAGA TGACAACCCG 
GACTTTGAAG GAGTGGATTG TGCAATCTTT GAGTCCCCGT ACCCGATGAC AATGGCGCTG 
TCTGTTCTAG TAACCATAGA GATGTGTAAC GCCCTCAACA GCTTGTCTGA AAACCAGTCT 
CTGCTGAGGA TGCCCCCTTG GGAGAACATC TGGCTCGTGG GCTCCATCTG CCTGTCCATG 
TCGCTCCATT TCCTCATCCT CTACGTGGAA CCTTTGCCAC TTATTTTCCA GATCACACCG 
CTGAACTTGA CCCAGTGGCT GATGGTGCTG AAAATCTCCT TGCCTGTGAT TCTAATGGAT 
GAGACACTCA AGTTTGTGGC CCGCAACTAC CTGGAACCTG CAATACTGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026900008.1
CDS	154..3147
Translation	MENAHTKTVEEVLGYFGVNESTGLSLEQVKKLKERWGSNELPAEEGKTLLELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILVANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDRKSVQRIKAKDIVPGDIVEIAVGDKVPADIRLTSIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFSGTNIAAGKAMGVVVATGVNTEIGKIRDEMVATEQERTPLQQKLDEFGEQLSKVISLICIAVWIINIGHFNDPVHGGSWIRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDKVEGDTCSLNEFTITGSTYAPIGEVHKDDKPVKCHQYDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTPGVKQKVMSVIREWGSGSDTLRCLALATHDNPLRREEMNLEDSANFIKYETNLTFVGCVGMLDPPRIEVASSVKLCRQAGIRVIMITGDNKGTAVAICRRIGIFGQDEDVTSKAFTGREFDELSPSAQRDACLNARCFARVEPSHKSKIVEFLQSFDEITAMTGDGVNDAPALKKSEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMNKPPRNPKEPLISGWLFFRYLAIGCYVGAATVGAAAWWFIAADGGPRVSFYQLSHFLQCKDDNPDFEGVDCAIFESPYPMTMALSVLVTIEMCNALNSLSENQSLLRMPPWENIWLVGSICLSMSLHFLILYVEPLPLIFQITPLNLTQWLMVLKISLPVILMDETLKFVARNYLEPAILE

Target ORF information:

RefSeq Version	XM_027044207.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (ATP2A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027044207.1

ORF Insert Sequence:

ATGGAGAACG CGCACACAAA GACGGTGGAG GAGGTGCTGG GCTACTTCGG CGTCAACGAG 
AGCACAGGGC TAAGCCTGGA GCAGGTCAAG AAGCTCAAGG AGAGATGGGG CTCCAATGAG 
TTACCGGCTG AAGAAGGAAA AACCTTGTTG GAACTTGTGA TTGAGCAGTT TGAAGACTTA 
TTAGTTAGAA TTTTATTGCT GGCAGCTTGT ATATCTTTTG TTTTGGCTTG GTTTGAAGAA 
GGTGAAGAAA CAATTACAGC CTTTGTAGAA CCCTTTGTAA TTTTACTTAT ATTAGTAGCC 
AATGCTATTG TGGGTGTGTG GCAGGAAAGA AATGCTGAGA ATGCTATTGA AGCCCTTAAG 
GAATATGAGC CTGAAATGGG CAAAGTATAT CGACAGGACA GAAAGAGTGT ACAGCGGATT 
AAAGCTAAGG ACATAGTTCC TGGTGATATT GTAGAAATTG CTGTTGGTGA CAAAGTTCCT 
GCTGATATAA GATTAACTTC CATCAAATCT ACTACTCTGA GAGTTGACCA GTCCATTCTC 
ACAGGTGAGT CTGTGTCTGT CATCAAGCAC ACTGACCCTG TCCCAGACCC ACGGGCCGTC 
AACCAAGATA AGAAGAACAT GCTGTTTTCT GGAACAAACA TTGCTGCTGG CAAAGCCATG 
GGAGTGGTGG TGGCAACTGG AGTTAACACA GAAATCGGCA AGATCCGGGA TGAGATGGTG 
GCAACAGAAC AGGAGAGGAC ACCCCTCCAG CAGAAACTAG ACGAGTTTGG GGAGCAGCTT 
TCCAAAGTCA TATCCCTTAT TTGCATTGCA GTCTGGATCA TAAACATTGG GCACTTCAAT 
GACCCAGTTC ACGGAGGCTC CTGGATCAGG GGTGCTATTT ACTACTTTAA AATTGCGGTG 
GCCCTGGCTG TGGCGGCCAT TCCTGAAGGT CTGCCTGCTG TCATCACCAC CTGCCTGGCT 
CTTGGAACTC GCAGAATGGC AAAGAAAAAT GCCATTGTTC GAAGTCTCCC TTCTGTGGAA 
ACCCTTGGTT GTACTTCTGT TATCTGCTCA GACAAGACTG GTACGCTTAC GACGAACCAG 
ATGTCAGTGT GCAGGATGTT CATTCTGGAC AAGGTTGAGG GGGATACTTG TTCCCTTAAT 
GAGTTTACCA TAACTGGGTC AACATATGCA CCTATTGGAG AAGTGCATAA AGATGATAAA 
CCAGTGAAAT GTCATCAATA TGATGGTCTT GTGGAATTGG CAACAATTTG TGCTCTTTGT 
AATGATTCTG CATTGGATTA CAATGAGGCA AAGGGCGTGT ATGAAAAAGT TGGGGAAGCT 
ACAGAGACTG CTCTCACTTG CCTAGTAGAG AAGATGAATG TATTTGATAC CGAATTGAAG 
GGTCTTTCTA AAATAGAAAG AGCAAATGCC TGCAACTCGG TCATAAAGCA GTTAATGAAA 
AAAGAATTTA CTCTCGAGTT TTCACGCGAT AGAAAATCAA TGTCAGTTTA TTGTACACCA 
AATAAACCAA GCCGGACATC GATGAGCAAA ATGTTTGTGA AGGGCGCTCC CGAAGGTGTC 
ATTGACAGGT GCACCCACAT TCGGGTTGGA AGTACCAAAG TCCCTATGAC CCCTGGAGTC 
AAACAGAAGG TCATGTCTGT CATCAGGGAA TGGGGTAGTG GCAGCGACAC ACTGCGGTGC 
CTGGCTCTGG CCACTCATGA CAACCCACTG AGAAGAGAAG AAATGAACCT TGAGGACTCT 
GCCAACTTTA TCAAATATGA GACTAATCTG ACTTTCGTTG GCTGTGTGGG CATGCTGGAC 
CCTCCAAGAA TTGAAGTGGC CTCTTCTGTG AAGCTTTGCC GGCAAGCAGG CATCCGGGTG 
ATCATGATCA CAGGGGACAA CAAAGGCACT GCTGTGGCCA TCTGTCGCCG CATTGGCATC 
TTTGGGCAGG ACGAGGATGT GACGTCAAAG GCTTTTACGG GTCGGGAGTT TGATGAGCTC 
AGTCCTTCAG CCCAGAGAGA TGCCTGCTTG AATGCCCGCT GTTTTGCTCG AGTTGAGCCT 
TCCCACAAGT CAAAAATTGT AGAGTTTCTT CAGTCTTTTG ATGAGATTAC AGCTATGACC 
GGGGATGGCG TGAATGATGC TCCTGCCCTG AAGAAATCGG AGATTGGCAT TGCCATGGGC 
TCTGGCACGG CAGTGGCTAA AACCGCCTCT GAGATGGTCC TGGCCGACGA CAACTTCTCC 
ACCATTGTGG CTGCTGTCGA GGAGGGGCGG GCGATTTACA ACAATATGAA GCAGTTTATC 
CGCTACCTCA TCTCTTCGAA TGTGGGGGAA GTTGTCTGTA TTTTCCTGAC TGCAGCCCTT 
GGATTTCCTG AGGCTTTAAT TCCTGTCCAG CTGCTCTGGG TCAATCTGGT GACAGACGGC 
CTGCCTGCCA CCGCACTGGG GTTTAACCCA CCTGATCTGG ACATCATGAA CAAACCACCC 
CGGAACCCGA AGGAACCACT GATCAGCGGA TGGCTCTTTT TCCGTTACCT AGCTATTGGC 
TGTTACGTTG GCGCTGCTAC TGTGGGTGCT GCCGCGTGGT GGTTTATTGC TGCCGACGGG 
GGTCCAAGGG TGTCCTTCTA CCAACTGAGC CACTTCCTAC AGTGTAAAGA TGACAACCCG 
GACTTTGAAG GAGTGGATTG TGCAATCTTT GAGTCCCCGT ACCCGATGAC AATGGCGCTG 
TCTGTTCTAG TAACCATAGA GATGTGTAAC GCCCTCAACA GCTTGTCTGA AAACCAGTCT 
CTGCTGAGGA TGCCCCCTTG GGAGAACATC TGGCTCGTGG GCTCCATCTG CCTGTCCATG 
TCGCTCCATT TCCTCATCCT CTACGTGGAA CCTTTGCCAC TTATTTTCCA GATCACACCG 
CTGAACTTGA CCCAGTGGCT GATGGTGCTG AAAATCTCCT TGCCTGTGAT TCTAATGGAT 
GAGACACTCA AGTTTGTGGC CCGCAACTAC CTGGAACCTG CAATACTGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP2A2 ( XM_015063763.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_014919249.1 Acinonyx jubatus ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (ATP2A2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc39411
Clone ID Related Accession (Same CDS sequence)	XM_015063763.1
Accession Version	XM_015063763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Acinonyx jubatus(cheetah)
Product	sarcoplasmic/endoplasmic reticulum calcium ATPase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015130765.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acinonyx jubatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTATTGT GGGTGTGTGG CAGGAAAGAA ATGCTGAGAA TGCTATTGAA GCCCTTAAGG 
AATATGAGCC TGAAATGGGC AAAGTATATC GACAGGACAG AAAGANNNNN TAAAGCTAAG 
GACATAGTTC CTGGTGATAT TGTAGAAATT GCTGTTGGTG ACAAAGTTCC TGCTNNTTTA 
ACTTCCATCA AATCTACTAC TCTGAGAGTT GACCAGTCCA TTCTCACAGG TGAGTCTGTG 
TCTGTCATCA AGCACACTGA CCCTGTCCCA GACCCACGGG CCGTCAACCA AGATAAGAAG 
AACATGCTGT TTTCTGGAAC AAACATTGCT GCTGGCAAAG CCATGGGAGT GGTGGTGGCA 
ACTGGAGTTA ACACAGAAAT CGGCAAGATC CGGGATGAGA TGGTGGCAAC AGAACAGGAG 
AGAACACCCC TCCAGCAGAA ACTAGACGAG TTTGGGGAGC AGCTTTCCAA AGTCATATCC 
CTTATTTGCA TTGCAGTCTG GATCATAAAC ATTGGGCACT TCAATGACCC AGTTCACGGA 
GGCTCCTGGA TCAGGGGTGC TATTTACTAC TTTAAAATTG CGGTGGCCCT GGCTGTGGCG 
GCCATTCCTG AAGGTCTGCC TGCTGTCATC ACCACCTGCC TGGCTCTTGG AACTCGCAGA 
ATGGCAAAGA AAAATGCCAT TGTTCGAAGT CTCCCTTCTG TGGAAACCCT TGGTTGTACT 
TCTGTTATCT GCTCAGACAA GACTGGTACG CTTACGACGA ACCAGATGTC AGTGTGCAGG 
ATGTTCATTC TGGACAAGGT TGAGGGGGAT ACTTGTTCCC TTAATGAGTT TACCATAACT 
GGGTCAACAT ATGCACCTAT TGGAGAAGTG CATAAAGATG ATAAACCAGT GAAATGTCAT 
CAATATGATG GTCTTGTGGA ATTGGCAACA ATTTGTGCTC TTTGTAATGA TTCTGCATTG 
GATTACAATG AGGCAAAGGG CGTGTATGAA AAAGTTGGGG AAGCTACAGA GACTGCTCTC 
ACTTGCCTAG TAGAGAAGAT GAATGTATTT GATACCGAAT TGAAGGGTCT TTCTAAAATA 
GAAAGAGCAA ATGCCTGCAA CTCGGTCATA AAGCAGTTAA TGAAAAAAGA ATTTACTCTC 
GAGTTTTCAC GCGATAGAAA ATCAATGTCA GTTTATTGTA CACCAAATAA ACCAAGCCGG 
ACATCGATGA GCAAAATGTT TGTGAAGGGC GCTCCCGAAG GTGTCATTGA CAGGTGCACC 
CACATTCGGG TTGGAAGTAC CAAAGTCCCT ATGACCCCTG GAGTCAAACA GAAGGTCATG 
TCTGTCATCA GGGAATGGGG TAGTGGCAGC GACACACTGC GGTGCCTGGC TCTGGCCACT 
CATGACAACC CACTGAGAAG AGAAGAAATG AACCTTGAGG ACTCTGCCAA CTTTATCAAA 
TATGAGACTA ATCTGACTTT CGTTGGCTGT GTGGGCATGC TGGACCCTCC AAGAATTGAA 
GTGGCCTCTT CTGTGAAGCT TTGCCGGCAA GCAGGCATCC GGGTGATCAT GATCACAGGG 
GACAACAAAG GCACTGCTGT GGCCATCTGT CGCCGCATTG GCATCTTTGG GCAGGACGAG 
GATGTGACGT CAAAGGCTTT TACGGGTCGG GAGTTTGATG AGCTCAGTCC TTCAGCCCAG 
AGAGATGCCT GCTTGAATGC CCGCTGTTTT GCTCGAGTTG AGCCTTCCCA CAAGTCAAAA 
ATTGTAGAGT TTCTTCAGTC TTTTGATGAG ATTACAGCTA TGACCGGGGA TGGCGTGAAT 
GATGCTCCTG CCCTGAAGAA ATCGGAGATT GGCATTGCCA TGGGCTCTGG CACGGCAGTG 
GCTAAAACCG CCTCTGAGAT GGTCCTGGCC GACGACAACT TCTCCACCAT TGTGGCTGCT 
GTCGAGGAGG GGCGGGCGAT TTACAACAAT ATGAAGCAGT TTATCCGCTA CCTCATCTCT 
TCAAATGTGG GGGAAGTTGT CTGTATTTTC CTGACTGCAG CCCTTGGATT TCCTGAGGCT 
TTAATTCCTG TCCAGCTGCT CTGGGTCAAT CTGGTGACAG ACGGCCTGCC TGCCACCGCA 
CTGGGGTTTA ACCCACCTGA TCTGGACATC ATGAACAAAC CACCCCGGAA CCCGAAGGAA 
CCACTGATCA GCGGATGGCT CTTTTTCCGT TACCTAGCTA TTGGCTGTTA CGTTGGCGCT 
GCTACTGTGG GTGCTGCCGC GTGGTGGTTT ATTGCTGCCG ACGGGGGTCC AAGGGTGTCC 
TTCTACCAAC TGAGCCACTT CCTACAGTGT AAAGATGACA ACCCGGACTT TGAAGGAGTG 
GATTGTGCAA TCTTTGAGTC CCCGTACCCG ATGACAATGG CGCTGTCTGT TCTAGTAACC 
ATAGAGATGT GTAACGCCCT CAACAGCTTG TCTGAAAACC AGTCTCTGCT GAGGATGCCC 
CCTTGGGAGA ACATCTGGCT CGTGGGCTCC ATCTGCCTGT CCATGTCGCT CCATTTCCTC 
ATCCTCTACG TGGAACCTTT GCCACTTATT TTCCAGATCA CACCGCTGAA CTTGACCCAG 
TGGCTGATGG TGCTGAAAAT CTCCTTGCCT GTGATTCTAA TGGATGAGAC ACTCAAGTTT 
GTGGCCCGCA ACTACCTGGA ACCTGCAATA CTGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014919249.1
CDS	185..2863
Translation	MLLWVCGRKEMLRMLLKPLRNMSLKWAKYIDRTERnullnullKAKDIVPGDIVEIAVGDKVPAnullLTSIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFSGTNIAAGKAMGVVVATGVNTEIGKIRDEMVATEQERTPLQQKLDEFGEQLSKVISLICIAVWIINIGHFNDPVHGGSWIRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDKVEGDTCSLNEFTITGSTYAPIGEVHKDDKPVKCHQYDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTPGVKQKVMSVIREWGSGSDTLRCLALATHDNPLRREEMNLEDSANFIKYETNLTFVGCVGMLDPPRIEVASSVKLCRQAGIRVIMITGDNKGTAVAICRRIGIFGQDEDVTSKAFTGREFDELSPSAQRDACLNARCFARVEPSHKSKIVEFLQSFDEITAMTGDGVNDAPALKKSEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMNKPPRNPKEPLISGWLFFRYLAIGCYVGAATVGAAAWWFIAADGGPRVSFYQLSHFLQCKDDNPDFEGVDCAIFESPYPMTMALSVLVTIEMCNALNSLSENQSLLRMPPWENIWLVGSICLSMSLHFLILYVEPLPLIFQITPLNLTQWLMVLKISLPVILMDETLKFVARNYLEPAILE

Target ORF information:

RefSeq Version	XM_015063763.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (ATP2A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015063763.1

ORF Insert Sequence:

ATGCTATTGT GGGTGTGTGG CAGGAAAGAA ATGCTGAGAA TGCTATTGAA GCCCTTAAGG 
AATATGAGCC TGAAATGGGC AAAGTATATC GACAGGACAG AAAGANNNNN TAAAGCTAAG 
GACATAGTTC CTGGTGATAT TGTAGAAATT GCTGTTGGTG ACAAAGTTCC TGCTNNTTTA 
ACTTCCATCA AATCTACTAC TCTGAGAGTT GACCAGTCCA TTCTCACAGG TGAGTCTGTG 
TCTGTCATCA AGCACACTGA CCCTGTCCCA GACCCACGGG CCGTCAACCA AGATAAGAAG 
AACATGCTGT TTTCTGGAAC AAACATTGCT GCTGGCAAAG CCATGGGAGT GGTGGTGGCA 
ACTGGAGTTA ACACAGAAAT CGGCAAGATC CGGGATGAGA TGGTGGCAAC AGAACAGGAG 
AGAACACCCC TCCAGCAGAA ACTAGACGAG TTTGGGGAGC AGCTTTCCAA AGTCATATCC 
CTTATTTGCA TTGCAGTCTG GATCATAAAC ATTGGGCACT TCAATGACCC AGTTCACGGA 
GGCTCCTGGA TCAGGGGTGC TATTTACTAC TTTAAAATTG CGGTGGCCCT GGCTGTGGCG 
GCCATTCCTG AAGGTCTGCC TGCTGTCATC ACCACCTGCC TGGCTCTTGG AACTCGCAGA 
ATGGCAAAGA AAAATGCCAT TGTTCGAAGT CTCCCTTCTG TGGAAACCCT TGGTTGTACT 
TCTGTTATCT GCTCAGACAA GACTGGTACG CTTACGACGA ACCAGATGTC AGTGTGCAGG 
ATGTTCATTC TGGACAAGGT TGAGGGGGAT ACTTGTTCCC TTAATGAGTT TACCATAACT 
GGGTCAACAT ATGCACCTAT TGGAGAAGTG CATAAAGATG ATAAACCAGT GAAATGTCAT 
CAATATGATG GTCTTGTGGA ATTGGCAACA ATTTGTGCTC TTTGTAATGA TTCTGCATTG 
GATTACAATG AGGCAAAGGG CGTGTATGAA AAAGTTGGGG AAGCTACAGA GACTGCTCTC 
ACTTGCCTAG TAGAGAAGAT GAATGTATTT GATACCGAAT TGAAGGGTCT TTCTAAAATA 
GAAAGAGCAA ATGCCTGCAA CTCGGTCATA AAGCAGTTAA TGAAAAAAGA ATTTACTCTC 
GAGTTTTCAC GCGATAGAAA ATCAATGTCA GTTTATTGTA CACCAAATAA ACCAAGCCGG 
ACATCGATGA GCAAAATGTT TGTGAAGGGC GCTCCCGAAG GTGTCATTGA CAGGTGCACC 
CACATTCGGG TTGGAAGTAC CAAAGTCCCT ATGACCCCTG GAGTCAAACA GAAGGTCATG 
TCTGTCATCA GGGAATGGGG TAGTGGCAGC GACACACTGC GGTGCCTGGC TCTGGCCACT 
CATGACAACC CACTGAGAAG AGAAGAAATG AACCTTGAGG ACTCTGCCAA CTTTATCAAA 
TATGAGACTA ATCTGACTTT CGTTGGCTGT GTGGGCATGC TGGACCCTCC AAGAATTGAA 
GTGGCCTCTT CTGTGAAGCT TTGCCGGCAA GCAGGCATCC GGGTGATCAT GATCACAGGG 
GACAACAAAG GCACTGCTGT GGCCATCTGT CGCCGCATTG GCATCTTTGG GCAGGACGAG 
GATGTGACGT CAAAGGCTTT TACGGGTCGG GAGTTTGATG AGCTCAGTCC TTCAGCCCAG 
AGAGATGCCT GCTTGAATGC CCGCTGTTTT GCTCGAGTTG AGCCTTCCCA CAAGTCAAAA 
ATTGTAGAGT TTCTTCAGTC TTTTGATGAG ATTACAGCTA TGACCGGGGA TGGCGTGAAT 
GATGCTCCTG CCCTGAAGAA ATCGGAGATT GGCATTGCCA TGGGCTCTGG CACGGCAGTG 
GCTAAAACCG CCTCTGAGAT GGTCCTGGCC GACGACAACT TCTCCACCAT TGTGGCTGCT 
GTCGAGGAGG GGCGGGCGAT TTACAACAAT ATGAAGCAGT TTATCCGCTA CCTCATCTCT 
TCAAATGTGG GGGAAGTTGT CTGTATTTTC CTGACTGCAG CCCTTGGATT TCCTGAGGCT 
TTAATTCCTG TCCAGCTGCT CTGGGTCAAT CTGGTGACAG ACGGCCTGCC TGCCACCGCA 
CTGGGGTTTA ACCCACCTGA TCTGGACATC ATGAACAAAC CACCCCGGAA CCCGAAGGAA 
CCACTGATCA GCGGATGGCT CTTTTTCCGT TACCTAGCTA TTGGCTGTTA CGTTGGCGCT 
GCTACTGTGG GTGCTGCCGC GTGGTGGTTT ATTGCTGCCG ACGGGGGTCC AAGGGTGTCC 
TTCTACCAAC TGAGCCACTT CCTACAGTGT AAAGATGACA ACCCGGACTT TGAAGGAGTG 
GATTGTGCAA TCTTTGAGTC CCCGTACCCG ATGACAATGG CGCTGTCTGT TCTAGTAACC 
ATAGAGATGT GTAACGCCCT CAACAGCTTG TCTGAAAACC AGTCTCTGCT GAGGATGCCC 
CCTTGGGAGA ACATCTGGCT CGTGGGCTCC ATCTGCCTGT CCATGTCGCT CCATTTCCTC 
ATCCTCTACG TGGAACCTTT GCCACTTATT TTCCAGATCA CACCGCTGAA CTTGACCCAG 
TGGCTGATGG TGCTGAAAAT CTCCTTGCCT GTGATTCTAA TGGATGAGAC ACTCAAGTTT 
GTGGCCCGCA ACTACCTGGA ACCTGCAATA CTGGAGTAA