ACA1_112040 cDNA ORF clone, Acanthamoeba castellanii str. Neff

Gene
Clones
gRNAs

Summary

Gene Symbol	ACA1_112040
Entrez Gene ID	14920768
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Acanthamoeba castellanii str. Neff

mRNA and Protein(s)

mRNA	Protein	Name
XM_004341991.1	XP_004342040.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling.
Clarke M, Lohan AJ, Liu B, Lagkouvardos I, Roy S, Zafar N, Bertelli C, Schilde C, Kianianmomeni A, B?rglin TR, Frech C, Turcotte B, Kopec KO, Synnott JM, Choo C, Paponov I, Finkler A, Heng Tan CS, Hutchins AP, Weinmeier T, Rattei T, Chu JS, Gimenez G, Irimia M, Rigden DJ, Fitzpatrick DA, Lorenzo-Morales J, Bateman A, Chiu CH, Tang P, Hegemann P, Fromm H, Raoult D, Greub G, Miranda-Saavedra D, Chen N, Nash P, Ginger ML, Horn M, Schaap P, Caler L, Loftus BJ
Genome biology14(2)R11(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACA1_112040 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACA1_112040 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc109431	XM_004341991.1 Latest version!	Acanthamoeba castellanii str. Neff hypothetical protein (ACA1_112040) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACA1_112040 ( XM_004341991.1 ) cDNA ORF clone, Acanthamoeba castellanii str. Neff -> XP_004342040.1 Acanthamoeba castellanii str. Neff hypothetical protein (ACA1_112040) mRNA, complete cds.

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CloneID	OAc109431
Clone ID Related Accession (Same CDS sequence)	XM_004341991.1
Accession Version	XM_004341991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-03-26
Organism	Acanthamoeba castellanii str. Neff
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_004457554). Long-term curation of the sequence and subsequent annotation updates will be the responsibility of the EuPathDB Bioinformatics Resource Center http://EuPathDB.org. Annotation can be viewed at http://AmoebaDB.org and inquires can be sent to [email protected]. COMPLETENESS: incomplete on both ends.

ATGGCCCTCA AGCCCAAACC CCAGGCCGCG GCCTTGTTCG AGGGTCTTGC CATCAAGGAA 
GAAGAAGAAG AAGAGACCAC ATCCACAACC ACGGCCTCGG GTGGCGGCTC GTCTTCGTTT 
TCGTTCATCA ACGAGGAGCC CCAGGGAGAA GAAGAAGATG AAGGCGCAGG CGGGTCGCCG 
GGCGGCGGGG CCGTGCGCAA GAGGAAGGCG GCCAAGCGGC CCGGCACGTA CTACCAGGAG 
GGCGATCAGG TCAAGCCAGC CGCCGCTGCG GCCACGGCCG AAGCGAGGAG CGCCAAACAG 
CCGGCAACGA CGCCGCGCAA GGAGGAGAGC GACAAGCGAA AGGACGAAAG CAAGGCGGCG 
GAGGAGGACA CGTCGGCCAA ACGCGAACGA GAAGAAGCGG CGAAGCGGCG GCAGAGGGAG 
AAGGAGGAGG AAGAACGAAA GCGACAAAGG CAGAAGGAAG AAGAAGAAGA ACGAAAGCGA 
CAGGAAAGCG AAGAAGATAG GGAGGAGGCC GCGAGGCAGC TGCAGCGCAG GAAGGAGGCG 
GACGAACGAT CGAAGGGCTC GAGCCAGCCC GACAAGAAAT ACGAAAAGGT GATCGCGAAC 
GCGAGACGGG AATTCGCTTC TTTGGACGCC GAGCGCGAAT CGCTGACCCA CCAGCAGAAG 
ACCATCCTCA GCGAACAGCG GGAGCGAAAC CGAGTCACGC TCAACTTGCA AGAACAGCTG 
ATCAAGCTGG ATGCCGAGCA AGTGCAAGCG CTTCAGCTCG AGGACTTCGA ACGAGCCGAG 
AAATTGAACG AAAAGATTGA AGGCACGCAC GCACACTCGC ATCCTTACTT GACCGCGCGC 
TCAAAGTTAT TGTTTGATGT TGATGATTCT CTTCTGCACG TCAAACAAAA TAGAGGTCAA 
GGAGGAGATG AAGAAGATCG CACGGCAGAA GCTGCAGTTC AGCCAACAGG TGCTGGTCTT 
GCATGTGAAG AGGGGCCAGG CGGCGCAGAA GGAAGCGCAG CTACGACGGA GCTTCATCGA 
CAAGCTCAAG GAACTGAAGA CGGAAGAGGA GGAACAGCTG GAAGCATTCA AGAGCCAGAC 
GAGCAGCAAA TTCTCGGAAG AGAAGGAGCG CATCCAGGCA AAGAAGAAGG GCATCCAGAA 
GCATCTCACT CACGTGCGCC TGGACCTGGA GATGTTGCTC AAGAGCGAAG AGACGGTGCA 
CCAGTCCATC GCGTCCCAGA CGCAGAGCTT CAAGGAGGAC CTGCACAAGC TCAACCAAGA 
TCGCGACGCC ACGGAGATCA AGGAGCTGAT GCGGCTGCTG GCCCAGAAGC GGAAAGAGGA 
GGAAGAGCTG ACCAGGCAGA TCCACGGGCT GGAGCGGAAC GTCGTGCAGG TGACCGACAG 
GTTCAAGAAC GAGATGGAGC GGATCGGCAT CGAGCGAGAC GCCATCGCCA AGGACAACCA 
CAAATACTCC CTCCAAATGG AGGAGCTGAA GAAGGCGAAG CGGCAGCTGA AGGGCGAGGA 
GGCGAAGGAC AGGGAGGCGG AGAAGCGCTT CCACGACGCG CTCACTGCGA TCGAGGCCGA 
GCTGACCAAC GAGCGCGCGG CGGTCGACAA ACTCGAGCAG AAGGAGGACG AGCTGCGAAG 
GGCCAAGACC AACTACGAGG CCTTCGTCCG AATGGAGGCC GATCAAGAAG AGCAGACCTC 
GGCGTGCAGC GCGGCCATCA CCCAACTCAC CGCGCGAGTG CTCTCTCTGC AGCAATACAC 
GGCGCCAATC AGCAAATTGT CGCCTTGGAT GCTCGCATCC CCTCGCTTGA ATCCGAGAAG 
AAACTCGCTG TGTCCGGACG TAATTTCAAG GAGGCCAAGA GAATCGCCGA AGAGATGAAG 
ACGGCCTCGG TACAGATTGA AGAGAGCAAG GACACCGTCG CTGCCCTCAC GGAGCAGCTT 
GAGACCTGCA AGGTCACTCT GTCGCAGCAC AAGGAACAGT TCAAATCGCT CGACCAAGAG 
CTCAGTTCAG CCGAGAGCAA AGCCGACAAG GTCAAGCTCC AGCGGCTCAA GGCCAAGGAG 
AAGGAGCTGC TCTCCAAGCT GGCCAGGTAC CACGGCCAGG ACGAGGAGAC GGAGATGTCG 
GCGCTCGAGC TCGAGTCCTG CCAGGCCGAG ATGGCCTCCC TCCGGCTGAA GTGGAACCTC 
CCCCAGCACG ACGATGACGA CAACGACGAA GCTCTCCGCG AGAGCGAAAG CGAAAGCAAA 
CGAAAGAGTC GAGGAGACAC CACTGGAATC AAAGAGCGCG AAAGCGAAAG CGACAAACGC 
GCCCCGGAGG CCGAGAGGGA AGACCAAAAG AGCGAAAGCG AAGAGCAGGG CGAGCGTGCG 
GACCCGGCGT TCGATGCGGC CGCGGCGGAA GAGGAGCTGA GGACCCTGGC CGCGCGCCTG 
GAGGTGTGGG AGGCCGAGCT CGAGCAGTGC GTGGCCAACG ACGACTTTGA CCGAGCTGAC 
GAGGTCAACC AGGAGATCGA GCGGGCCAAG GCGCGGTCGG CCGTCCTCCA AGCGGCGCTC 
CCCGCGAGCA GCAGCTCCTC GAGTGATCCA GCGCCGACGC AAACGTCGAA TTCGTCGCCT 
GACGAAGAGC CATCGGCCTT CGCCTCCATT CCTCGCGCTG AAGATGGCGG TGACGTCGGT 
GGCGGTGGCG ATGATGGCGA CGAGGCGTTT AAGTCCGATC GAGGTGTGGT CGAGGAAGAG 
GCTGATCAGC CGAGCGTGGA GCGGGAGGTG GAGGTGGAGG CCTCGGGTGA CGATGGAGCG 
GACCAGGCCG GGTTCGCGTT TATCTCCGAA GAGGAAGAAG AGGCACCAAA GAACGAAGAC 
GAAAACGAAA ATGAAGACGA AGACGAAGAC GAAGACGTTG GCGACGCAGA GCAAGCCGCT 
GCGGTGGTGG AAGAGGGCGC CGACTCGTCC TCCTCCTCCT CCTTCGCCTT CATGGCGCAC 
GACGACAACG AAGAGGAAGG AGAGGAAGAG AAGAAGGACA ATGTGGAGGA GTCAGAGAAG 
ATCACGGACG AGGCGGCGCC AGCGGAGGAA GTGGAGCAGC TGCCACCGGT CGACACATCT 
GCGGCCTCGT TTGCGTTCAT CATCGGAGAC GACGAAGACG AGGACATCAG CCACAACAGC 
GACAACAACG ACCACAAGGA GGAGGAGAGC GGTGGCCACG CCTTCTCCTT CATCGCCGCC 
AGCGAAGAAG AGGCAGCACC ACCAGCACCA GCACCCACGG CAGACGCAGG CAAGGAAGAA 
GTGGAGGAGA TCGAAGCAGG AGGAGGAGGA GAAGAGGGTG AGTCCTCGTC TGCCTTCTCC 
TTCATATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004342040.1
CDS	1..3312
Translation	MALKPKPQAAALFEGLAIKEEEEEETTSTTTASGGGSSSFSFINEEPQGEEEDEGAGGSPGGGAVRKRKAAKRPGTYYQEGDQVKPAAAAATAEARSAKQPATTPRKEESDKRKDESKAAEEDTSAKREREEAAKRRQREKEEEERKRQRQKEEEEERKRQESEEDREEAARQLQRRKEADERSKGSSQPDKKYEKVIANARREFASLDAERESLTHQQKTILSEQRERNRVTLNLQEQLIKLDAEQVQALQLEDFERAEKLNEKIEGTHAHSHPYLTARSKLLFDVDDSLLHVKQNRGQGGDEEDRTAEAAVQPTGAGLACEEGPGGAEGSAATTELHRQAQGTEDGRGGTAGSIQEPDEQQILGREGAHPGKEEGHPEASHSRAPGPGDVAQERRDGAPVHRVPDAELQGGPAQAQPRSRRHGDQGADAAAGPEAERGGRADQADPRAGAERRAGDRQVQERDGADRHRARRHRQGQPQILPPNGGAEEGEAAAEGRGGEGQGGGEALPRRAHCDRGRADQRARGGRQTRAEGGRAAKGQDQLRGLRPNGGRSRRADLGVQRGHHPTHRASALSAAIHGANQQIVALDARIPSLESEKKLAVSGRNFKEAKRIAEEMKTASVQIEESKDTVAALTEQLETCKVTLSQHKEQFKSLDQELSSAESKADKVKLQRLKAKEKELLSKLARYHGQDEETEMSALELESCQAEMASLRLKWNLPQHDDDDNDEALRESESESKRKSRGDTTGIKERESESDKRAPEAEREDQKSESEEQGERADPAFDAAAAEEELRTLAARLEVWEAELEQCVANDDFDRADEVNQEIERAKARSAVLQAALPASSSSSSDPAPTQTSNSSPDEEPSAFASIPRAEDGGDVGGGGDDGDEAFKSDRGVVEEEADQPSVEREVEVEASGDDGADQAGFAFISEEEEEAPKNEDENENEDEDEDEDVGDAEQAAAVVEEGADSSSSSSFAFMAHDDNEEEGEEEKKDNVEESEKITDEAAPAEEVEQLPPVDTSAASFAFIIGDDEDEDISHNSDNNDHKEEESGGHAFSFIAASEEEAAPPAPAPTADAGKEEVEEIEAGGGGEEGESSSAFSFIS

Target ORF information:

RefSeq Version	XM_004341991.1
Organism	Acanthamoeba castellanii str. Neff
Definition	Acanthamoeba castellanii str. Neff hypothetical protein (ACA1_112040) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004341991.1

ORF Insert Sequence:

ATGGCCCTCA AGCCCAAACC CCAGGCCGCG GCCTTGTTCG AGGGTCTTGC CATCAAGGAA 
GAAGAAGAAG AAGAGACCAC ATCCACAACC ACGGCCTCGG GTGGCGGCTC GTCTTCGTTT 
TCGTTCATCA ACGAGGAGCC CCAGGGAGAA GAAGAAGATG AAGGCGCAGG CGGGTCGCCG 
GGCGGCGGGG CCGTGCGCAA GAGGAAGGCG GCCAAGCGGC CCGGCACGTA CTACCAGGAG 
GGCGATCAGG TCAAGCCAGC CGCCGCTGCG GCCACGGCCG AAGCGAGGAG CGCCAAACAG 
CCGGCAACGA CGCCGCGCAA GGAGGAGAGC GACAAGCGAA AGGACGAAAG CAAGGCGGCG 
GAGGAGGACA CGTCGGCCAA ACGCGAACGA GAAGAAGCGG CGAAGCGGCG GCAGAGGGAG 
AAGGAGGAGG AAGAACGAAA GCGACAAAGG CAGAAGGAAG AAGAAGAAGA ACGAAAGCGA 
CAGGAAAGCG AAGAAGATAG GGAGGAGGCC GCGAGGCAGC TGCAGCGCAG GAAGGAGGCG 
GACGAACGAT CGAAGGGCTC GAGCCAGCCC GACAAGAAAT ACGAAAAGGT GATCGCGAAC 
GCGAGACGGG AATTCGCTTC TTTGGACGCC GAGCGCGAAT CGCTGACCCA CCAGCAGAAG 
ACCATCCTCA GCGAACAGCG GGAGCGAAAC CGAGTCACGC TCAACTTGCA AGAACAGCTG 
ATCAAGCTGG ATGCCGAGCA AGTGCAAGCG CTTCAGCTCG AGGACTTCGA ACGAGCCGAG 
AAATTGAACG AAAAGATTGA AGGCACGCAC GCACACTCGC ATCCTTACTT GACCGCGCGC 
TCAAAGTTAT TGTTTGATGT TGATGATTCT CTTCTGCACG TCAAACAAAA TAGAGGTCAA 
GGAGGAGATG AAGAAGATCG CACGGCAGAA GCTGCAGTTC AGCCAACAGG TGCTGGTCTT 
GCATGTGAAG AGGGGCCAGG CGGCGCAGAA GGAAGCGCAG CTACGACGGA GCTTCATCGA 
CAAGCTCAAG GAACTGAAGA CGGAAGAGGA GGAACAGCTG GAAGCATTCA AGAGCCAGAC 
GAGCAGCAAA TTCTCGGAAG AGAAGGAGCG CATCCAGGCA AAGAAGAAGG GCATCCAGAA 
GCATCTCACT CACGTGCGCC TGGACCTGGA GATGTTGCTC AAGAGCGAAG AGACGGTGCA 
CCAGTCCATC GCGTCCCAGA CGCAGAGCTT CAAGGAGGAC CTGCACAAGC TCAACCAAGA 
TCGCGACGCC ACGGAGATCA AGGAGCTGAT GCGGCTGCTG GCCCAGAAGC GGAAAGAGGA 
GGAAGAGCTG ACCAGGCAGA TCCACGGGCT GGAGCGGAAC GTCGTGCAGG TGACCGACAG 
GTTCAAGAAC GAGATGGAGC GGATCGGCAT CGAGCGAGAC GCCATCGCCA AGGACAACCA 
CAAATACTCC CTCCAAATGG AGGAGCTGAA GAAGGCGAAG CGGCAGCTGA AGGGCGAGGA 
GGCGAAGGAC AGGGAGGCGG AGAAGCGCTT CCACGACGCG CTCACTGCGA TCGAGGCCGA 
GCTGACCAAC GAGCGCGCGG CGGTCGACAA ACTCGAGCAG AAGGAGGACG AGCTGCGAAG 
GGCCAAGACC AACTACGAGG CCTTCGTCCG AATGGAGGCC GATCAAGAAG AGCAGACCTC 
GGCGTGCAGC GCGGCCATCA CCCAACTCAC CGCGCGAGTG CTCTCTCTGC AGCAATACAC 
GGCGCCAATC AGCAAATTGT CGCCTTGGAT GCTCGCATCC CCTCGCTTGA ATCCGAGAAG 
AAACTCGCTG TGTCCGGACG TAATTTCAAG GAGGCCAAGA GAATCGCCGA AGAGATGAAG 
ACGGCCTCGG TACAGATTGA AGAGAGCAAG GACACCGTCG CTGCCCTCAC GGAGCAGCTT 
GAGACCTGCA AGGTCACTCT GTCGCAGCAC AAGGAACAGT TCAAATCGCT CGACCAAGAG 
CTCAGTTCAG CCGAGAGCAA AGCCGACAAG GTCAAGCTCC AGCGGCTCAA GGCCAAGGAG 
AAGGAGCTGC TCTCCAAGCT GGCCAGGTAC CACGGCCAGG ACGAGGAGAC GGAGATGTCG 
GCGCTCGAGC TCGAGTCCTG CCAGGCCGAG ATGGCCTCCC TCCGGCTGAA GTGGAACCTC 
CCCCAGCACG ACGATGACGA CAACGACGAA GCTCTCCGCG AGAGCGAAAG CGAAAGCAAA 
CGAAAGAGTC GAGGAGACAC CACTGGAATC AAAGAGCGCG AAAGCGAAAG CGACAAACGC 
GCCCCGGAGG CCGAGAGGGA AGACCAAAAG AGCGAAAGCG AAGAGCAGGG CGAGCGTGCG 
GACCCGGCGT TCGATGCGGC CGCGGCGGAA GAGGAGCTGA GGACCCTGGC CGCGCGCCTG 
GAGGTGTGGG AGGCCGAGCT CGAGCAGTGC GTGGCCAACG ACGACTTTGA CCGAGCTGAC 
GAGGTCAACC AGGAGATCGA GCGGGCCAAG GCGCGGTCGG CCGTCCTCCA AGCGGCGCTC 
CCCGCGAGCA GCAGCTCCTC GAGTGATCCA GCGCCGACGC AAACGTCGAA TTCGTCGCCT 
GACGAAGAGC CATCGGCCTT CGCCTCCATT CCTCGCGCTG AAGATGGCGG TGACGTCGGT 
GGCGGTGGCG ATGATGGCGA CGAGGCGTTT AAGTCCGATC GAGGTGTGGT CGAGGAAGAG 
GCTGATCAGC CGAGCGTGGA GCGGGAGGTG GAGGTGGAGG CCTCGGGTGA CGATGGAGCG 
GACCAGGCCG GGTTCGCGTT TATCTCCGAA GAGGAAGAAG AGGCACCAAA GAACGAAGAC 
GAAAACGAAA ATGAAGACGA AGACGAAGAC GAAGACGTTG GCGACGCAGA GCAAGCCGCT 
GCGGTGGTGG AAGAGGGCGC CGACTCGTCC TCCTCCTCCT CCTTCGCCTT CATGGCGCAC 
GACGACAACG AAGAGGAAGG AGAGGAAGAG AAGAAGGACA ATGTGGAGGA GTCAGAGAAG 
ATCACGGACG AGGCGGCGCC AGCGGAGGAA GTGGAGCAGC TGCCACCGGT CGACACATCT 
GCGGCCTCGT TTGCGTTCAT CATCGGAGAC GACGAAGACG AGGACATCAG CCACAACAGC 
GACAACAACG ACCACAAGGA GGAGGAGAGC GGTGGCCACG CCTTCTCCTT CATCGCCGCC 
AGCGAAGAAG AGGCAGCACC ACCAGCACCA GCACCCACGG CAGACGCAGG CAAGGAAGAA 
GTGGAGGAGA TCGAAGCAGG AGGAGGAGGA GAAGAGGGTG AGTCCTCGTC TGCCTTCTCC 
TTCATATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling.
Genome biology14(2)R11(2013 Feb)
Clarke M,Lohan AJ,Liu B,Lagkouvardos I,Roy S,Zafar N,Bertelli C,Schilde C,Kianianmomeni A,B?rglin TR,Frech C,Turcotte B,Kopec KO,Synnott JM,Choo C,Paponov I,Finkler A,Heng Tan CS,Hutchins AP,Weinmeier T,Rattei T,Chu JS,Gimenez G,Irimia M,Rigden DJ,Fitzpatrick DA,Lorenzo-Morales J,Bateman A,Chiu CH,Tang P,Hegemann P,Fromm H,Raoult D,Greub G,Miranda-Saavedra D,Chen N,Nash P,Ginger ML,Horn M,Schaap P,Caler L,Loftus BJ

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ACA1_112040 cDNA ORF clone, Acanthamoeba castellanii str. Neff

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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