TNF cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	TNF
Entrez Gene ID	403922
Full Name	tumor necrosis factor
Synonyms	TNFA,TNLG1F,cTNF
General protein information	Preferred Names tumor necrosis factor Names tumor necrosis factor TNF-a TNF-alpha cachectin tumor necrosis factor (TNF superfamily, member 2) tumor necrosis factor ligand 1F tumor necrosis factor ligand superfamily member 2 tumor necrosis factor-alpha tumour necrosis factor
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003244.4	NP_001003244.4	tumor necrosis factor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tnf	NP_038721.1
Canis lupus familiaris (dog)	TNF	NP_001003244.4
Bos taurus (cattle)	TNF	NP_776391.2
Rattus norvegicus (Norway rat)	Tnf	NP_036807.1
	TNF	NP_001040614.1
Xenopus tropicalis (tropical clawed frog)	tnf	NP_001107143.1
Homo sapiens (human)	TNF	NP_000585.2
Pan troglodytes (chimpanzee)	TNF	NP_001038976.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence analysis of three canine adipokine genes revealed an association between TNF polymorphisms and obesity in Labrador dogs.
Mankowska M, Stachowiak M, Graczyk A, Ciazynska P, Gogulski M, Nizanski W, Switonski M
Animal genetics47(2)245-9(2016 Apr)

Comparative genomic analysis of eutherian tumor necrosis factor ligand genes.
Premzl M
Immunogenetics68(2)125-32(2016 Feb)

Expression Profile of Cytokines and Enzymes mRNA in Blood Leukocytes of Dogs with Leptospirosis and Its Associated Pulmonary Hemorrhage Syndrome.
Maissen-Villiger CA, Schweighauser A, van Dorland HA, Morel C, Bruckmaier RM, Zurbriggen A, Francey T
PloS one11(1)e0148029(2016)

Propofol attenuates LPS-induced tumor necrosis factor-?, interleukin-6 and nitric oxide expression in canine peripheral blood mononuclear cells possibly through down-regulation of nuclear factor (NF)-?B activation.
Pei Z, Wang J
The Journal of veterinary medical science77(2)139-45(2015 Feb)

Characterization of a canine tetranucleotide microsatellite marker located in the first intron of the tumor necrosis factor alpha gene.
Watanabe M, Tanaka K, Takizawa T, Segawa K, Neo S, Tsuchiya R, Murata M, Murakami M, Hisasue M
The Journal of veterinary medical science76(1)119-22(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

two SNPs of the TNF gene are associated with obesity in Labrador dogs
Title: Sequence analysis of three canine adipokine genes revealed an association between TNF polymorphisms and obesity in Labrador dogs.

Fatal outcome from pulmonary hemorrhages in dogs with leptospirosis was associated with low TNF-alpha, high IL-1beta, and high iNOS expression.
Title: Expression Profile of Cytokines and Enzymes mRNA in Blood Leukocytes of Dogs with Leptospirosis and Its Associated Pulmonary Hemorrhage Syndrome.

This diminished TNF-alpha, IL-6 and iNOS expression, and NO production was in parallel to the respective decreased NF-kappaB p65 protein nuclear translocation in the LPS-activated canine PBMCs pretreated with 25 microM and 50 microM propofol.
Title: Propofol attenuates LPS-induced tumor necrosis factor-?, interleukin-6 and nitric oxide expression in canine peripheral blood mononuclear cells possibly through down-regulation of nuclear factor (NF)-?B activation.

Tumour necrosis factor (TNF)-alpha expression was observed in vascular endothelial cells in both neoplastic and normal kidney.
Title: Angiogenesis and expression of vascular endothelial growth factor, tumour necrosis factor-? and hypoxia inducible factor-1? in canine renal cell carcinoma.

The observed HMGB1 release kinetics showed a clearly time-dependent manner with a peak release 24h after TNF-alpha stimulation, while stimulation with IFN-gamma had only small effects on the HMGB1 release.
Title: TNF-? induced secretion of HMGB1 from non-immune canine mammary epithelial cells (MTH53A).

The following TNF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe34299	NM_001003244.4 Latest version!	Canis lupus familiaris tumor necrosis factor (TNF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNF ( NM_001003244.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003244.4 Canis lupus familiaris tumor necrosis factor (TNF), mRNA.

Price & Availability↑

CloneID	OCe34299
Clone ID Related Accession (Same CDS sequence)	NM_001003244.4
Accession Version	NM_001003244.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Canis lupus familiaris(dog)
Product	tumor necrosis factor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ923808.1. On or before Oct 30, 2006 this sequence version replaced XM_844179.1, XM_853289.1, XM_853330.1, XM_853367.1, NM_001003244.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ923808.1, S74068.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4547739, SAMEA4547740 [ECO:0000348] ##Evidence-Data-END##

ATGAGCACTG AAAGCATGAT CCGGGACGTG GAGCTGGCCG AGGAGCCGCT CCCCAAGAAG 
GCGGGGGGCC CCCCGGGCTC CAGAAGGTGC TTCTGCCTCA GCCTCTTCTC CTTCCTCCTC 
GTCGCAGGGG CCACCACACT CTTCTGCCTG CTGCACTTTG GAGTGATCGG CCCCCAGAGG 
GAAGAGCTCC CAAATGGCCT CCAACTAATC AGCCCTCTTG CCCAGACAGT CAAATCATCT 
TCTCGAACCC CAAGTGACAA GCCAGTAGCT CATGTTGTAG CAAACCCCGA AGCTGAGGGG 
CAGCTCCAGT GGCTGAGCCG ACGTGCCAAT GCCCTCCTGG CCAACGGCGT GGAGCTGACA 
GACAACCAGC TGATAGTGCC GTCAGATGGG TTGTACCTCA TCTACTCCCA GGTCCTCTTC 
AAGGGCCAAG GGTGCCCTTC CACCCATGTG CTCCTCACCC ACACCATCAG CCGCTTCGCC 
GTCTCCTACC AGACAAAGGT CAACCTACTC TCTGCCATCA AGAGCCCTTG CCAAAGGGAG 
ACCCCAGAGG GGACCGAGGC CAAGCCCTGG TACGAGCCCA TCTACCTGGG AGGGGTCTTC 
CAACTGGAGA AGGGTGATCG ACTCAGCGCT GAGATCAATC TGCCTAACTA TCTGGACTTT 
GCCGAGTCCG GGCAGGTGTA CTTTGGAATC ATTGCCCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003244.4
CDS	1..702
Translation	MSTESMIRDVELAEEPLPKKAGGPPGSRRCFCLSLFSFLLVAGATTLFCLLHFGVIGPQREELPNGLQLISPLAQTVKSSSRTPSDKPVAHVVANPEAEGQLQWLSRRANALLANGVELTDNQLIVPSDGLYLIYSQVLFKGQGCPSTHVLLTHTISRFAVSYQTKVNLLSAIKSPCQRETPEGTEAKPWYEPIYLGGVFQLEKGDRLSAEINLPNYLDFAESGQVYFGIIAL

Target ORF information:

RefSeq Version	NM_001003244.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tumor necrosis factor (TNF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003244.4

ORF Insert Sequence:

ATGAGCACTG AAAGCATGAT CCGGGACGTG GAGCTGGCCG AGGAGCCGCT CCCCAAGAAG 
GCGGGGGGCC CCCCGGGCTC CAGAAGGTGC TTCTGCCTCA GCCTCTTCTC CTTCCTCCTC 
GTCGCAGGGG CCACCACACT CTTCTGCCTG CTGCACTTTG GAGTGATCGG CCCCCAGAGG 
GAAGAGCTCC CAAATGGCCT CCAACTAATC AGCCCTCTTG CCCAGACAGT CAAATCATCT 
TCTCGAACCC CAAGTGACAA GCCAGTAGCT CATGTTGTAG CAAACCCCGA AGCTGAGGGG 
CAGCTCCAGT GGCTGAGCCG ACGTGCCAAT GCCCTCCTGG CCAACGGCGT GGAGCTGACA 
GACAACCAGC TGATAGTGCC GTCAGATGGG TTGTACCTCA TCTACTCCCA GGTCCTCTTC 
AAGGGCCAAG GGTGCCCTTC CACCCATGTG CTCCTCACCC ACACCATCAG CCGCTTCGCC 
GTCTCCTACC AGACAAAGGT CAACCTACTC TCTGCCATCA AGAGCCCTTG CCAAAGGGAG 
ACCCCAGAGG GGACCGAGGC CAAGCCCTGG TACGAGCCCA TCTACCTGGG AGGGGTCTTC 
CAACTGGAGA AGGGTGATCG ACTCAGCGCT GAGATCAATC TGCCTAACTA TCTGGACTTT 
GCCGAGTCCG GGCAGGTGTA CTTTGGAATC ATTGCCCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence analysis of three canine adipokine genes revealed an association between TNF polymorphisms and obesity in Labrador dogs.
Animal genetics47(2)245-9(2016 Apr)
Mankowska M,Stachowiak M,Graczyk A,Ciazynska P,Gogulski M,Nizanski W,Switonski M

Comparative genomic analysis of eutherian tumor necrosis factor ligand genes.
Immunogenetics68(2)125-32(2016 Feb)
Premzl M

Expression Profile of Cytokines and Enzymes mRNA in Blood Leukocytes of Dogs with Leptospirosis and Its Associated Pulmonary Hemorrhage Syndrome.
PloS one11(1)e0148029(2016)
Maissen-Villiger CA,Schweighauser A,van Dorland HA,Morel C,Bruckmaier RM,Zurbriggen A,Francey T

Propofol attenuates LPS-induced tumor necrosis factor-?, interleukin-6 and nitric oxide expression in canine peripheral blood mononuclear cells possibly through down-regulation of nuclear factor (NF)-?B activation.
The Journal of veterinary medical science77(2)139-45(2015 Feb)
Pei Z,Wang J

Characterization of a canine tetranucleotide microsatellite marker located in the first intron of the tumor necrosis factor alpha gene.
The Journal of veterinary medical science76(1)119-22(2014 Jan)
Watanabe M,Tanaka K,Takizawa T,Segawa K,Neo S,Tsuchiya R,Murata M,Murakami M,Hisasue M