LOC103457431 cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103457431
Entrez Gene ID	103457431
Full Name	calpain-3-like
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008397544.2	XP_008395766.1	calpain-3-like isoform X1
XM_008397545.2	XP_008395767.1	calpain-3-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC103457431 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103457431 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo187558	XM_008397544.2 Latest version!	Poecilia reticulata calpain-3-like (LOC103457431), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPo187559	XM_008397545.2 Latest version!	Poecilia reticulata calpain-3-like (LOC103457431), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103457431 ( XM_008397544.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008395766.1 Poecilia reticulata calpain-3-like (LOC103457431), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo187558
Clone ID Related Accession (Same CDS sequence)	XM_008397544.2
Accession Version	XM_008397544.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	calpain-3-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024351.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008397544.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG GGAACCATCC AGAGAAGGAA CCTGTGGTTG AGAAAACAAA GGTGAAAGTG 
CTTCTTGACA CTGAGGCGTC ACTGGAGGCT GATGGCAATG CTGACTTCGG GATCACAGGA 
GCAAACTCCA TCTACTCGGC CATTCTCAGC AGAAACGAGG CCGTCAAAGA TGAAAAGCGC 
CTGAAGACCT TTTTGGAGCT GAGGGATAAA TATGTTACGA AGCATGTTCT TTTTGAGGAC 
CCTCTCTTCC CTGCAGACGA CTCCTCACTT TTTTACAGCG AGAAACCCAC AATGAAGTTC 
GAGTGGAAAC GTCCCTCAGA AATCTGTGAA GATCCCCAGT TCATCGTCGA TGGGGCCAAC 
AGAACCGACA TCTGCCAAGG AACTCTGGGG GACTGCTGGC TGCTCGCCGC CATCGCCTGC 
CTGACTCTGA ACGAGAAGCT GCTGTACAGA GTGATTCCTC CGGACCAGAG CTTCACTGAG 
AATTACGCCG GGATCTTCCA CTTTCAGTTC TGGCGTTATG GTGAATGGAT CGACGTGGTT 
GTGGATGACC GCATCCCCAC CTGCAACAAC CAGCTGGTTT TCACCAAATC TTTTAGAAAG 
AACGAGTTCT GGAGCGCTTT GTTGGAAAAA GCTTACGCCA AGTTGAACGG ATCTTATGAG 
GCACTGAAAG GGGGAAACAC GTTGGAAGCC ATGGAGGATT TCACAGGTGG AGTCACTGAG 
TTTTTTGAGC TGTCCGAGGC ACCTGCTGAT CTCTTCACGA TCATGAGGAA GGCGCTGGAG 
AGGGGCTCGC TGATGGGCAG CTCCATAGAT GTTTCGTCCG CCAGTGAAAT GGAGTCCCGG 
ACTGACCATG GGCTGGTCAG GGGTCACGCC TACTCCATCA TCGGCCTGGA AGAGTGCGAC 
GAAGTCTCAA AGGACTCCAG AATCCGCCTG ATTCGTCTAC GCAATCCGTG GGGAATTGTG 
CTCTGGAAGG GTCCCTGGAG TGCCAAGTCA GATCAGTGGT CGACTATTTC TACTGCAGAC 
AGGGAGAATC TGAAGAAACA GACAGTGGAG ACAAGTGAGT TCTGGATGTC CTTTGATGAG 
TTCAAGAAGA CTTTCACCAA ACTGGAGATG TGTAACCTCA CCCCCGACGG ACTGCAGGGC 
GACGAACGGA TGACCTGGAC AGTTTCGGTC AATGAAGGTC GCTGGGTGAG AGGCAGCTCT 
GCTGGTGGCT GCAGGAACTT CCCAGACACA TTTTGGACAA ACCCTCAGTA TCGGCTTCAG 
CTTTATGAGG AGGATGACGA CCCAGAGGAC GGGCAGGTGG CCTGCACTGT TGTTGTAGCC 
CTGATGCAAA AGGGTCGTAG GATGCAGCGC CATAAAGGAG CAAAATTCCT TACCGTTGGA 
TTTTCTATCT ACAAGGTGCC AGAGAAGATG CAAGGCCAGA ATCAACATCT GCAGAAGGAC 
TTCTTCCTCT ACACAGCCTC CACAGCCAAA TGCAAGAGCT ACATCAACCT GCGGGAGGTG 
ACGGAGCGTT TCCGACTGCC TCCGGGAGAG TATGCCATCA TTCCCACGAC CTTTGAACCC 
CACGAGGAGG GAGAGTTCAT CCTCAGGGTC TTCTCTGAGA AGCAGAGCAC ATCTGAGGAA 
GTAGGGAGCA CCATCGATCA AATAGAGCAA GTCAAGAAAA AGAAGCCTAT TGTATTTGTA 
TCAGACAGAG CGCGCGCCAA CAAAGAAATT GAGCATGATG GCATTCGGGG GGAAAAGAGG 
AAGAAACCAA AGCGGAAACT GCTGCAAGCC GAGGAAGAGA CTGAAGAAGA GAAAAAGTTC 
AGAGCCATTT ACAAACAGAT TGCAGGCGAG GACATGGAGA TCTGTGCAAA CGAACTCAAG 
ACGATCATGA AGAACGTTCT TTCTAAACAT AGTGAGATAA AGACAGAAGG CTTCAGCCTC 
GAGACGTGTC GAAGCATGAT CGCCTTAATG GATACTGATG GATCGGGGAA ACTGAATTTC 
CAGGAATTCA AACACTTGTG GAAAAAGATA AAAGCATGGC AGCTCATCTT CAAACAGTAC 
GATCAGAACA AGTCCTGCTC CATCAGCAGC TTCGAGATGA GGAACGCCGT CAATGATGCA 
GGGTTTCACC TCAACAAACA GTTGTACGAC ATCATAGCCA TGCGATACGC AGACGAACAC 
CTCAACATTG ACTTTGACAG CTACATCTGC TGTTTTGTGA GACTAGAGGG AATGTTCAGG 
GCCTTTAATG CCTTTGATAA AGACGGAGAC GGAATAATTA AACTAAACGT CCTGGAGTGG 
CTTCAGCTGA CGATGTATTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008395766.1
CDS	201..2504
Translation	MEDGNHPEKEPVVEKTKVKVLLDTEASLEADGNADFGITGANSIYSAILSRNEAVKDEKRLKTFLELRDKYVTKHVLFEDPLFPADDSSLFYSEKPTMKFEWKRPSEICEDPQFIVDGANRTDICQGTLGDCWLLAAIACLTLNEKLLYRVIPPDQSFTENYAGIFHFQFWRYGEWIDVVVDDRIPTCNNQLVFTKSFRKNEFWSALLEKAYAKLNGSYEALKGGNTLEAMEDFTGGVTEFFELSEAPADLFTIMRKALERGSLMGSSIDVSSASEMESRTDHGLVRGHAYSIIGLEECDEVSKDSRIRLIRLRNPWGIVLWKGPWSAKSDQWSTISTADRENLKKQTVETSEFWMSFDEFKKTFTKLEMCNLTPDGLQGDERMTWTVSVNEGRWVRGSSAGGCRNFPDTFWTNPQYRLQLYEEDDDPEDGQVACTVVVALMQKGRRMQRHKGAKFLTVGFSIYKVPEKMQGQNQHLQKDFFLYTASTAKCKSYINLREVTERFRLPPGEYAIIPTTFEPHEEGEFILRVFSEKQSTSEEVGSTIDQIEQVKKKKPIVFVSDRARANKEIEHDGIRGEKRKKPKRKLLQAEEETEEEKKFRAIYKQIAGEDMEICANELKTIMKNVLSKHSEIKTEGFSLETCRSMIALMDTDGSGKLNFQEFKHLWKKIKAWQLIFKQYDQNKSCSISSFEMRNAVNDAGFHLNKQLYDIIAMRYADEHLNIDFDSYICCFVRLEGMFRAFNAFDKDGDGIIKLNVLEWLQLTMYS

Target ORF information:

RefSeq Version	XM_008397544.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata calpain-3-like (LOC103457431), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008397544.2

ORF Insert Sequence:

ATGGAGGACG GGAACCATCC AGAGAAGGAA CCTGTGGTTG AGAAAACAAA GGTGAAAGTG 
CTTCTTGACA CTGAGGCGTC ACTGGAGGCT GATGGCAATG CTGACTTCGG GATCACAGGA 
GCAAACTCCA TCTACTCGGC CATTCTCAGC AGAAACGAGG CCGTCAAAGA TGAAAAGCGC 
CTGAAGACCT TTTTGGAGCT GAGGGATAAA TATGTTACGA AGCATGTTCT TTTTGAGGAC 
CCTCTCTTCC CTGCAGACGA CTCCTCACTT TTTTACAGCG AGAAACCCAC AATGAAGTTC 
GAGTGGAAAC GTCCCTCAGA AATCTGTGAA GATCCCCAGT TCATCGTCGA TGGGGCCAAC 
AGAACCGACA TCTGCCAAGG AACTCTGGGG GACTGCTGGC TGCTCGCCGC CATCGCCTGC 
CTGACTCTGA ACGAGAAGCT GCTGTACAGA GTGATTCCTC CGGACCAGAG CTTCACTGAG 
AATTACGCCG GGATCTTCCA CTTTCAGTTC TGGCGTTATG GTGAATGGAT CGACGTGGTT 
GTGGATGACC GCATCCCCAC CTGCAACAAC CAGCTGGTTT TCACCAAATC TTTTAGAAAG 
AACGAGTTCT GGAGCGCTTT GTTGGAAAAA GCTTACGCCA AGTTGAACGG ATCTTATGAG 
GCACTGAAAG GGGGAAACAC GTTGGAAGCC ATGGAGGATT TCACAGGTGG AGTCACTGAG 
TTTTTTGAGC TGTCCGAGGC ACCTGCTGAT CTCTTCACGA TCATGAGGAA GGCGCTGGAG 
AGGGGCTCGC TGATGGGCAG CTCCATAGAT GTTTCGTCCG CCAGTGAAAT GGAGTCCCGG 
ACTGACCATG GGCTGGTCAG GGGTCACGCC TACTCCATCA TCGGCCTGGA AGAGTGCGAC 
GAAGTCTCAA AGGACTCCAG AATCCGCCTG ATTCGTCTAC GCAATCCGTG GGGAATTGTG 
CTCTGGAAGG GTCCCTGGAG TGCCAAGTCA GATCAGTGGT CGACTATTTC TACTGCAGAC 
AGGGAGAATC TGAAGAAACA GACAGTGGAG ACAAGTGAGT TCTGGATGTC CTTTGATGAG 
TTCAAGAAGA CTTTCACCAA ACTGGAGATG TGTAACCTCA CCCCCGACGG ACTGCAGGGC 
GACGAACGGA TGACCTGGAC AGTTTCGGTC AATGAAGGTC GCTGGGTGAG AGGCAGCTCT 
GCTGGTGGCT GCAGGAACTT CCCAGACACA TTTTGGACAA ACCCTCAGTA TCGGCTTCAG 
CTTTATGAGG AGGATGACGA CCCAGAGGAC GGGCAGGTGG CCTGCACTGT TGTTGTAGCC 
CTGATGCAAA AGGGTCGTAG GATGCAGCGC CATAAAGGAG CAAAATTCCT TACCGTTGGA 
TTTTCTATCT ACAAGGTGCC AGAGAAGATG CAAGGCCAGA ATCAACATCT GCAGAAGGAC 
TTCTTCCTCT ACACAGCCTC CACAGCCAAA TGCAAGAGCT ACATCAACCT GCGGGAGGTG 
ACGGAGCGTT TCCGACTGCC TCCGGGAGAG TATGCCATCA TTCCCACGAC CTTTGAACCC 
CACGAGGAGG GAGAGTTCAT CCTCAGGGTC TTCTCTGAGA AGCAGAGCAC ATCTGAGGAA 
GTAGGGAGCA CCATCGATCA AATAGAGCAA GTCAAGAAAA AGAAGCCTAT TGTATTTGTA 
TCAGACAGAG CGCGCGCCAA CAAAGAAATT GAGCATGATG GCATTCGGGG GGAAAAGAGG 
AAGAAACCAA AGCGGAAACT GCTGCAAGCC GAGGAAGAGA CTGAAGAAGA GAAAAAGTTC 
AGAGCCATTT ACAAACAGAT TGCAGGCGAG GACATGGAGA TCTGTGCAAA CGAACTCAAG 
ACGATCATGA AGAACGTTCT TTCTAAACAT AGTGAGATAA AGACAGAAGG CTTCAGCCTC 
GAGACGTGTC GAAGCATGAT CGCCTTAATG GATACTGATG GATCGGGGAA ACTGAATTTC 
CAGGAATTCA AACACTTGTG GAAAAAGATA AAAGCATGGC AGCTCATCTT CAAACAGTAC 
GATCAGAACA AGTCCTGCTC CATCAGCAGC TTCGAGATGA GGAACGCCGT CAATGATGCA 
GGGTTTCACC TCAACAAACA GTTGTACGAC ATCATAGCCA TGCGATACGC AGACGAACAC 
CTCAACATTG ACTTTGACAG CTACATCTGC TGTTTTGTGA GACTAGAGGG AATGTTCAGG 
GCCTTTAATG CCTTTGATAA AGACGGAGAC GGAATAATTA AACTAAACGT CCTGGAGTGG 
CTTCAGCTGA CGATGTATTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103457431 ( XM_008397545.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008395767.1 Poecilia reticulata calpain-3-like (LOC103457431), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo187559
Clone ID Related Accession (Same CDS sequence)	XM_008397545.2
Accession Version	XM_008397545.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	calpain-3-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024351.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008397545.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG GGAACCATCC AGAGAAGGAA CCTGTGGTTG AGAAAACAAA GGTGAAAGTG 
CTTCTTGACA CTGAGGCGTC ACTGGAGGCT GATGGCAATG CTGACTTCGG GATCACAGGA 
GCAAACTCCA TCTACTCGGC CATTCTCAGC AGAAACGAGG CCGTCAAAGA TGAAAAGCGC 
CTGAAGACCT TTTTGGAGCT GAGGGATAAA TATGTTACGA AGCATGTTCT TTTTGAGGAC 
CCTCTCTTCC CTGCAGACGA CTCCTCACTT TTTTACAGCG AGAAACCCAC AATGAAGTTC 
GAGTGGAAAC GTCCCTCAGA AATCTGTGAA GATCCCCAGT TCATCGTCGA TGGGGCCAAC 
AGAACCGACA TCTGCCAAGG AACTCTGGGG GACTGCTGGC TGCTCGCCGC CATCGCCTGC 
CTGACTCTGA ACGAGAAGCT GCTGTACAGA GTGATTCCTC CGGACCAGAG CTTCACTGAG 
AATTACGCCG GGATCTTCCA CTTTCAGTTC TGGCGTTATG GTGAATGGAT CGACGTGGTT 
GTGGATGACC GCATCCCCAC CTGCAACAAC CAGCTGGTTT TCACCAAATC TTTTAGAAAG 
AACGAGTTCT GGAGCGCTTT GTTGGAAAAA GCTTACGCCA AGTTGAACGG ATCTTATGAG 
GCACTGAAAG GGGGAAACAC GTTGGAAGCC ATGGAGGATT TCACAGGTGG AGTCACTGAG 
TTTTTTGAGC TGTCCGAGGC ACCTGCTGAT CTCTTCACGA TCATGAGGAA GGCGCTGGAG 
AGGGGCTCGC TGATGGGCAG CTCCATAGAT GTTTCGTCCG CCAGTGAAAT GGAGTCCCGG 
ACTGACCATG GGCTGGTCAG GGGTCACGCC TACTCCATCA TCGGCCTGGA AGAGTGCGAC 
GAAGTCTCAA AGGACTCCAG AATCCGCCTG ATTCGTCTAC GCAATCCGTG GGGAATTGTG 
CTCTGGAAGG GTCCCTGGAG TGCCAAGTCA GATCAGTGGT CGACTATTTC TACTGCAGAC 
AGGGAGAATC TGAAGAAACA GACAGTGGAG ACAAGTGAGT TCTGGATGTC CTTTGATGAG 
TTCAAGAAGA CTTTCACCAA ACTGGAGATG TGTAACCTCA CCCCCGACGG ACTGCAGGGC 
GACGAACGGA TGACCTGGAC AGTTTCGGTC AATGAAGGTC GCTGGGTGAG AGGCAGCTCT 
GCTGGTGGCT GCAGGAACTT CCCAGACACA TTTTGGACAA ACCCTCAGTA TCGGCTTCAG 
CTTTATGAGG AGGATGACGA CCCAGAGGAC GGGCAGGTGG CCTGCACTGT TGTTGTAGCC 
CTGATGCAAA AGGGTCGTAG GATGCAGCGC CATAAAGGAG CAAAATTCCT TACCGTTGGA 
TTTTCTATCT ACAAGGTGCC AGAGAAGATG CAAGGCCAGA ATCAACATCT GCAGAAGGAC 
TTCTTCCTCT ACACAGCCTC CACAGCCAAA TGCAAGAGCT ACATCAACCT GCGGGAGGTG 
ACGGAGCGTT TCCGACTGCC TCCGGGAGAG TATGCCATCA TTCCCACGAC CTTTGAACCC 
CACGAGGAGG GAGAGTTCAT CCTCAGGGTC TTCTCTGAGA AGCAGAGCAC ATCTGAGGAA 
GTAGGGAGCA CCATCGATCA AATAGAGCAA GTCAAGAAAA AGAAGCCTAT TGTATTTGTA 
TCAGACAGAG CGCGCGCCAA CAAAGAAATT GAGCATGATG GCATTCGGGG GGAAAAGAGG 
AAGAAACCAA AGCGGAAACT GCTGCAAGCC GAGGAAGAGA CTGAAGAAGA GAAAAAGTTC 
AGAGCCATTT ACAAACAGAT TGCAGGCGAG GACATGGAGA TCTGTGCAAA CGAACTCAAG 
ACGATCATGA AGAACGTTCT TTCTAAACGT AAAGAGCTAC AATTTGTTTA CAGCACCCAT 
ACTGATGGAT CGGGGAAACT GAATTTCCAG GAATTCAAAC ACTTGTGGAA AAAGATAAAA 
GCATGGCAGC TCATCTTCAA ACAGTACGAT CAGAACAAGT CCTGCTCCAT CAGCAGCTTC 
GAGATGAGGA ACGCCGTCAA TGATGCAGGG TTTCACCTCA ACAAACAGTT GTACGACATC 
ATAGCCATGC GATACGCAGA CGAACACCTC AACATTGACT TTGACAGCTA CATCTGCTGT 
TTTGTGAGAC TAGAGGGAAT GTTCAGGGCC TTTAATGCCT TTGATAAAGA CGGAGACGGA 
ATAATTAAAC TAAACGTCCT GGAGTGGCTT CAGCTGACGA TGTATTCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008395767.1
CDS	201..2471
Translation	MEDGNHPEKEPVVEKTKVKVLLDTEASLEADGNADFGITGANSIYSAILSRNEAVKDEKRLKTFLELRDKYVTKHVLFEDPLFPADDSSLFYSEKPTMKFEWKRPSEICEDPQFIVDGANRTDICQGTLGDCWLLAAIACLTLNEKLLYRVIPPDQSFTENYAGIFHFQFWRYGEWIDVVVDDRIPTCNNQLVFTKSFRKNEFWSALLEKAYAKLNGSYEALKGGNTLEAMEDFTGGVTEFFELSEAPADLFTIMRKALERGSLMGSSIDVSSASEMESRTDHGLVRGHAYSIIGLEECDEVSKDSRIRLIRLRNPWGIVLWKGPWSAKSDQWSTISTADRENLKKQTVETSEFWMSFDEFKKTFTKLEMCNLTPDGLQGDERMTWTVSVNEGRWVRGSSAGGCRNFPDTFWTNPQYRLQLYEEDDDPEDGQVACTVVVALMQKGRRMQRHKGAKFLTVGFSIYKVPEKMQGQNQHLQKDFFLYTASTAKCKSYINLREVTERFRLPPGEYAIIPTTFEPHEEGEFILRVFSEKQSTSEEVGSTIDQIEQVKKKKPIVFVSDRARANKEIEHDGIRGEKRKKPKRKLLQAEEETEEEKKFRAIYKQIAGEDMEICANELKTIMKNVLSKRKELQFVYSTHTDGSGKLNFQEFKHLWKKIKAWQLIFKQYDQNKSCSISSFEMRNAVNDAGFHLNKQLYDIIAMRYADEHLNIDFDSYICCFVRLEGMFRAFNAFDKDGDGIIKLNVLEWLQLTMYS

Target ORF information:

RefSeq Version	XM_008397545.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata calpain-3-like (LOC103457431), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008397545.2

ORF Insert Sequence:

ATGGAGGACG GGAACCATCC AGAGAAGGAA CCTGTGGTTG AGAAAACAAA GGTGAAAGTG 
CTTCTTGACA CTGAGGCGTC ACTGGAGGCT GATGGCAATG CTGACTTCGG GATCACAGGA 
GCAAACTCCA TCTACTCGGC CATTCTCAGC AGAAACGAGG CCGTCAAAGA TGAAAAGCGC 
CTGAAGACCT TTTTGGAGCT GAGGGATAAA TATGTTACGA AGCATGTTCT TTTTGAGGAC 
CCTCTCTTCC CTGCAGACGA CTCCTCACTT TTTTACAGCG AGAAACCCAC AATGAAGTTC 
GAGTGGAAAC GTCCCTCAGA AATCTGTGAA GATCCCCAGT TCATCGTCGA TGGGGCCAAC 
AGAACCGACA TCTGCCAAGG AACTCTGGGG GACTGCTGGC TGCTCGCCGC CATCGCCTGC 
CTGACTCTGA ACGAGAAGCT GCTGTACAGA GTGATTCCTC CGGACCAGAG CTTCACTGAG 
AATTACGCCG GGATCTTCCA CTTTCAGTTC TGGCGTTATG GTGAATGGAT CGACGTGGTT 
GTGGATGACC GCATCCCCAC CTGCAACAAC CAGCTGGTTT TCACCAAATC TTTTAGAAAG 
AACGAGTTCT GGAGCGCTTT GTTGGAAAAA GCTTACGCCA AGTTGAACGG ATCTTATGAG 
GCACTGAAAG GGGGAAACAC GTTGGAAGCC ATGGAGGATT TCACAGGTGG AGTCACTGAG 
TTTTTTGAGC TGTCCGAGGC ACCTGCTGAT CTCTTCACGA TCATGAGGAA GGCGCTGGAG 
AGGGGCTCGC TGATGGGCAG CTCCATAGAT GTTTCGTCCG CCAGTGAAAT GGAGTCCCGG 
ACTGACCATG GGCTGGTCAG GGGTCACGCC TACTCCATCA TCGGCCTGGA AGAGTGCGAC 
GAAGTCTCAA AGGACTCCAG AATCCGCCTG ATTCGTCTAC GCAATCCGTG GGGAATTGTG 
CTCTGGAAGG GTCCCTGGAG TGCCAAGTCA GATCAGTGGT CGACTATTTC TACTGCAGAC 
AGGGAGAATC TGAAGAAACA GACAGTGGAG ACAAGTGAGT TCTGGATGTC CTTTGATGAG 
TTCAAGAAGA CTTTCACCAA ACTGGAGATG TGTAACCTCA CCCCCGACGG ACTGCAGGGC 
GACGAACGGA TGACCTGGAC AGTTTCGGTC AATGAAGGTC GCTGGGTGAG AGGCAGCTCT 
GCTGGTGGCT GCAGGAACTT CCCAGACACA TTTTGGACAA ACCCTCAGTA TCGGCTTCAG 
CTTTATGAGG AGGATGACGA CCCAGAGGAC GGGCAGGTGG CCTGCACTGT TGTTGTAGCC 
CTGATGCAAA AGGGTCGTAG GATGCAGCGC CATAAAGGAG CAAAATTCCT TACCGTTGGA 
TTTTCTATCT ACAAGGTGCC AGAGAAGATG CAAGGCCAGA ATCAACATCT GCAGAAGGAC 
TTCTTCCTCT ACACAGCCTC CACAGCCAAA TGCAAGAGCT ACATCAACCT GCGGGAGGTG 
ACGGAGCGTT TCCGACTGCC TCCGGGAGAG TATGCCATCA TTCCCACGAC CTTTGAACCC 
CACGAGGAGG GAGAGTTCAT CCTCAGGGTC TTCTCTGAGA AGCAGAGCAC ATCTGAGGAA 
GTAGGGAGCA CCATCGATCA AATAGAGCAA GTCAAGAAAA AGAAGCCTAT TGTATTTGTA 
TCAGACAGAG CGCGCGCCAA CAAAGAAATT GAGCATGATG GCATTCGGGG GGAAAAGAGG 
AAGAAACCAA AGCGGAAACT GCTGCAAGCC GAGGAAGAGA CTGAAGAAGA GAAAAAGTTC 
AGAGCCATTT ACAAACAGAT TGCAGGCGAG GACATGGAGA TCTGTGCAAA CGAACTCAAG 
ACGATCATGA AGAACGTTCT TTCTAAACGT AAAGAGCTAC AATTTGTTTA CAGCACCCAT 
ACTGATGGAT CGGGGAAACT GAATTTCCAG GAATTCAAAC ACTTGTGGAA AAAGATAAAA 
GCATGGCAGC TCATCTTCAA ACAGTACGAT CAGAACAAGT CCTGCTCCAT CAGCAGCTTC 
GAGATGAGGA ACGCCGTCAA TGATGCAGGG TTTCACCTCA ACAAACAGTT GTACGACATC 
ATAGCCATGC GATACGCAGA CGAACACCTC AACATTGACT TTGACAGCTA CATCTGCTGT 
TTTGTGAGAC TAGAGGGAAT GTTCAGGGCC TTTAATGCCT TTGATAAAGA CGGAGACGGA 
ATAATTAAAC TAAACGTCCT GGAGTGGCTT CAGCTGACGA TGTATTCCTA G