adam12 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	adam12
Entrez Gene ID	110529328
Full Name	ADAM metallopeptidase domain 12
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021611468.1	XP_021467143.1	disintegrin and metalloproteinase domain-containing protein 12 isoform X2
XM_021611461.1	XP_021467136.1	disintegrin and metalloproteinase domain-containing protein 12 isoform X1

Pathways from BioSystems
Gene Ontology

The following adam12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the adam12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf52957	XM_021611461.1 Latest version!	Oncorhynchus mykiss ADAM metallopeptidase domain 12 (adam12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OOf52958	XM_021611468.1 Latest version!	Oncorhynchus mykiss ADAM metallopeptidase domain 12 (adam12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

adam12 ( XM_021611461.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021467136.1 Oncorhynchus mykiss ADAM metallopeptidase domain 12 (adam12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOf52957
Clone ID Related Accession (Same CDS sequence)	XM_021611461.1
Accession Version	XM_021611461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	disintegrin and metalloproteinase domain-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035077.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAACT GGCGTTGGAC GAACTTTGCA TTTCTAATTG TTTTTCATGT CTTGTCCATG 
TCAACAGCAA CGGGCAATAC AGACAACACA CTTCTGAATT TACATGATGG GAAAAGCTTG 
GACCAGAGTG TGCTGCACAG TGCTGTCCCT AAGCCCCAAC GCTATGACAC CACGGTACCT 
CTCCTGCTGG AAGGGCGGCA GCGCAAACCA CTCAGCCAGC TCAAGTCCAG GAGTCATCCT 
GCATCACTGA AGCTGGTGGT GGAGGCGGAA GGTGAACAAC TGGTCCTGGC ACTAGAAAAA 
AACCAGGGCC TGTTTGCGAG CCATTACACT GAGACACATT ATCTGGACGA TGGCAGTGCA 
GTCACTGGTG CTCACAACTT TACGAAGAAC TGCTACTACC ATGGAGAAGT GGAGGGCCAC 
GCTAACTCTG ACGTCACTCT CAGCACCTGC TCTGGCCTCC GGGGTTTCAT CTCACTTGAA 
AATAAGACCT TCATCCTTGA ACCATCGTTG TACCAGGACA ATAATAAACA CATCATCTAC 
CGAGGGGAAA ACCTCAACCT GACCCCCGGA ACCTGTGGTC ATGGCTTCAA CATGTCCTCT 
GTTACCCCTG ACAACCATCT GAGAAGCCCT TTCAGAACAT TCAGTACCAG GAACAAGAGG 
CATGCTCAGA AAACTACCAA ATATGTAGAG CTGGTCATCG TTGCAGACAA CAGAGAGTTT 
CAGAAACAGG GGAAGGACAT TGAGAGGGTG AAGCAGAGAC TGGCAGAGAT AGCCAATTAC 
GTGGACAAGT TCTATAGGGT TCTGAACATC CGTGTGGCCC TAGTGGGCCT GGAGGTGTGG 
AGCGACGCTG ATAAGTGTCC TGTCACTCAG GACCCCTTCA ACACCCTGCA TGAGTTCCTA 
GACTGGAGGA AAGTCAAACT ACTGCCTACA AAATCACACG ACAACGCTCA GCTCATCAGT 
GGGGTGTACT TCCAGGGTAC CACCATTGGC ATGGCCCCCA TCATGAGCAT GTGTACAGTG 
GAGCAGTCTG GAGGCATCGT CATGGATCAT TCGGATAATC CCCTGGGCGC GGCTGTGACT 
TTAGCACATG AGCTGGGGCA CAACTTTGGC ATGAACCACG ACACTCCAGA GCGGGGGTGT 
GGCTGCAGGA TGACAGTGGA CCGCGGGGGC TGTATCATGA CCCCCTCCAC TGGGTACCCA 
TTTCCTACGG TATTCAGCAC CTGCAGTAAG AAGGACCTGG CAGCCAGTCT GGAGAAGGGA 
GTGGGGATGT GTCTGTACAA CATGCCTGAG GTCAAGGTGC TGTACGGGGG ACAGAAGTGT 
GGCAATGGAT ACGTGGAGGA GGGAGAGGAG TGTGACTGTG GAGAACTGGA GGAATGCATG 
AACCCCTGCT GCAATGCCAC CACCTGCACC CTGAAGGGAA ATGCCGTGTG CGCCCACGGA 
CAGTGCTGCG AGGACTGCAG GCTGAAGCCT GCTGGCACAC CGTGTCGAGA GTCCAGTAAC 
TCCTGTGATT TGCCAGAGTT CTGTACTGGT GCCAATCCCC ACTGCCCTGC CAACGTGTAC 
CTGCATGACG GTCACTCCTG CCACAACGTG GATGGATACT GCTACAACGG CATCTGCCAG 
ACTCATGAGC AACAGTGCAT CACGCTATGG GGACCAGGAG CCAAACCTGC CCCAGGGATC 
TGCTTTGAGA GGGTCAACTC AGCAGGAGAT CCTTACGGGA ACTGTGGGAA GGACTCCAAA 
GGATCGTTTG CCAAATGTGA AGCCCGAGAC GCAAAGTGTG GCAAAATTCA GTGTCAAGGA 
GGGGCCAACC GGCCTGTGAT TGGCACCAAT GCCGTCTCCA TAGAAACCAA CATCCCTCTT 
CAGGAAGGAG GGAGGATCCT GTGTCGGGGA ACCCACGTCT ACCTGGGTGA TGACATGCCC 
GACCCTGGAC TGGTTCTGGC TGGCACCAAG TGTGGAGAGG GCATGATGTG TCTGAATCGT 
CAGTGCCAGA ATGTCAGTGT GTTTGGAGTG CACCAGTGTT CAGGGAAGTG CAGCGGACGA 
GGGGTGTGTA ACAACTTGGA GAACTGCCAT TGTGAGGTGC ACTGGGCCCC TCCCTTCTGT 
GAGAGGGCGG GGTTTGGGGG CAGCCTAGAC AGTGGACCAA TGAGATTGGC AGATGTGCGT 
GTGTGTGTAT TAATGTGCGT GTTGTCGTAT GTGACATCAG CCGACAGCAG GGTGGTTGCT 
GTGGCAATCC TGGCCACCCT CCTCAGCCTT ATGGGTGCTG GGTTCCTGGT ATTCCTTAAG 
AGGAAGACTC TGGTCCGTCT GCTCTTCACA AACAAGAAAA CCACAATGGA GAAACTGAGA 
TCAGTTGGGT CGACCAGACC AGCCAGCCCC AACCAGACTC AGTCCATGTA CAGTCCACGA 
CGCAAGCCCC CAGCCAAGCC TCCAGGTACC AGCATATACA AGCCATCACA CCTGAGTTCG 
GAGCCTCTTC TCCCTCCACA CAGTTTCCAC TCCCACAGCT TGCGGCGGCT GCCCCACTAC 
CAGCCCCTCC ACATCAGCCA CCCCATGCCT GTCTCCCTGA GTGTGGGTCA GCAGCCCTTG 
CCACCGGTCC CGCCCCACCA CAAGGCTCTG CCGGCCCACA CCGCCCTCTC CCCACCACGG 
GCACCGCCCT TCCACAGCCT GCCCCGACAG CAGCCCTACA GAATGGACCA GGACTGCGAG 
AAACCCAGTC CACCCCAGAA GCCTCTGCCT GCAGACCCAC TGGGAAGGAG GGCTCGGGTG 
GGACGCAGCA CCTCGGTGGT GGGGGTTCGC CTCCCTGGAC CCATCCCTAT CCCCATCCCC 
ACCGTACCCA GACCCCTGCT CACCAATCCA CAACTGAACA GACCTGCACC CATACAACCC 
CTGAGACTGC CAAAGACTCA TCATTTTGAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021467136.1
CDS	454..3369
Translation	MTNWRWTNFAFLIVFHVLSMSTATGNTDNTLLNLHDGKSLDQSVLHSAVPKPQRYDTTVPLLLEGRQRKPLSQLKSRSHPASLKLVVEAEGEQLVLALEKNQGLFASHYTETHYLDDGSAVTGAHNFTKNCYYHGEVEGHANSDVTLSTCSGLRGFISLENKTFILEPSLYQDNNKHIIYRGENLNLTPGTCGHGFNMSSVTPDNHLRSPFRTFSTRNKRHAQKTTKYVELVIVADNREFQKQGKDIERVKQRLAEIANYVDKFYRVLNIRVALVGLEVWSDADKCPVTQDPFNTLHEFLDWRKVKLLPTKSHDNAQLISGVYFQGTTIGMAPIMSMCTVEQSGGIVMDHSDNPLGAAVTLAHELGHNFGMNHDTPERGCGCRMTVDRGGCIMTPSTGYPFPTVFSTCSKKDLAASLEKGVGMCLYNMPEVKVLYGGQKCGNGYVEEGEECDCGELEECMNPCCNATTCTLKGNAVCAHGQCCEDCRLKPAGTPCRESSNSCDLPEFCTGANPHCPANVYLHDGHSCHNVDGYCYNGICQTHEQQCITLWGPGAKPAPGICFERVNSAGDPYGNCGKDSKGSFAKCEARDAKCGKIQCQGGANRPVIGTNAVSIETNIPLQEGGRILCRGTHVYLGDDMPDPGLVLAGTKCGEGMMCLNRQCQNVSVFGVHQCSGKCSGRGVCNNLENCHCEVHWAPPFCERAGFGGSLDSGPMRLADVRVCVLMCVLSYVTSADSRVVAVAILATLLSLMGAGFLVFLKRKTLVRLLFTNKKTTMEKLRSVGSTRPASPNQTQSMYSPRRKPPAKPPGTSIYKPSHLSSEPLLPPHSFHSHSLRRLPHYQPLHISHPMPVSLSVGQQPLPPVPPHHKALPAHTALSPPRAPPFHSLPRQQPYRMDQDCEKPSPPQKPLPADPLGRRARVGRSTSVVGVRLPGPIPIPIPTVPRPLLTNPQLNRPAPIQPLRLPKTHHFDP

Target ORF information:

RefSeq Version	XM_021611461.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss ADAM metallopeptidase domain 12 (adam12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021611461.1

ORF Insert Sequence:

ATGACTAACT GGCGTTGGAC GAACTTTGCA TTTCTAATTG TTTTTCATGT CTTGTCCATG 
TCAACAGCAA CGGGCAATAC AGACAACACA CTTCTGAATT TACATGATGG GAAAAGCTTG 
GACCAGAGTG TGCTGCACAG TGCTGTCCCT AAGCCCCAAC GCTATGACAC CACGGTACCT 
CTCCTGCTGG AAGGGCGGCA GCGCAAACCA CTCAGCCAGC TCAAGTCCAG GAGTCATCCT 
GCATCACTGA AGCTGGTGGT GGAGGCGGAA GGTGAACAAC TGGTCCTGGC ACTAGAAAAA 
AACCAGGGCC TGTTTGCGAG CCATTACACT GAGACACATT ATCTGGACGA TGGCAGTGCA 
GTCACTGGTG CTCACAACTT TACGAAGAAC TGCTACTACC ATGGAGAAGT GGAGGGCCAC 
GCTAACTCTG ACGTCACTCT CAGCACCTGC TCTGGCCTCC GGGGTTTCAT CTCACTTGAA 
AATAAGACCT TCATCCTTGA ACCATCGTTG TACCAGGACA ATAATAAACA CATCATCTAC 
CGAGGGGAAA ACCTCAACCT GACCCCCGGA ACCTGTGGTC ATGGCTTCAA CATGTCCTCT 
GTTACCCCTG ACAACCATCT GAGAAGCCCT TTCAGAACAT TCAGTACCAG GAACAAGAGG 
CATGCTCAGA AAACTACCAA ATATGTAGAG CTGGTCATCG TTGCAGACAA CAGAGAGTTT 
CAGAAACAGG GGAAGGACAT TGAGAGGGTG AAGCAGAGAC TGGCAGAGAT AGCCAATTAC 
GTGGACAAGT TCTATAGGGT TCTGAACATC CGTGTGGCCC TAGTGGGCCT GGAGGTGTGG 
AGCGACGCTG ATAAGTGTCC TGTCACTCAG GACCCCTTCA ACACCCTGCA TGAGTTCCTA 
GACTGGAGGA AAGTCAAACT ACTGCCTACA AAATCACACG ACAACGCTCA GCTCATCAGT 
GGGGTGTACT TCCAGGGTAC CACCATTGGC ATGGCCCCCA TCATGAGCAT GTGTACAGTG 
GAGCAGTCTG GAGGCATCGT CATGGATCAT TCGGATAATC CCCTGGGCGC GGCTGTGACT 
TTAGCACATG AGCTGGGGCA CAACTTTGGC ATGAACCACG ACACTCCAGA GCGGGGGTGT 
GGCTGCAGGA TGACAGTGGA CCGCGGGGGC TGTATCATGA CCCCCTCCAC TGGGTACCCA 
TTTCCTACGG TATTCAGCAC CTGCAGTAAG AAGGACCTGG CAGCCAGTCT GGAGAAGGGA 
GTGGGGATGT GTCTGTACAA CATGCCTGAG GTCAAGGTGC TGTACGGGGG ACAGAAGTGT 
GGCAATGGAT ACGTGGAGGA GGGAGAGGAG TGTGACTGTG GAGAACTGGA GGAATGCATG 
AACCCCTGCT GCAATGCCAC CACCTGCACC CTGAAGGGAA ATGCCGTGTG CGCCCACGGA 
CAGTGCTGCG AGGACTGCAG GCTGAAGCCT GCTGGCACAC CGTGTCGAGA GTCCAGTAAC 
TCCTGTGATT TGCCAGAGTT CTGTACTGGT GCCAATCCCC ACTGCCCTGC CAACGTGTAC 
CTGCATGACG GTCACTCCTG CCACAACGTG GATGGATACT GCTACAACGG CATCTGCCAG 
ACTCATGAGC AACAGTGCAT CACGCTATGG GGACCAGGAG CCAAACCTGC CCCAGGGATC 
TGCTTTGAGA GGGTCAACTC AGCAGGAGAT CCTTACGGGA ACTGTGGGAA GGACTCCAAA 
GGATCGTTTG CCAAATGTGA AGCCCGAGAC GCAAAGTGTG GCAAAATTCA GTGTCAAGGA 
GGGGCCAACC GGCCTGTGAT TGGCACCAAT GCCGTCTCCA TAGAAACCAA CATCCCTCTT 
CAGGAAGGAG GGAGGATCCT GTGTCGGGGA ACCCACGTCT ACCTGGGTGA TGACATGCCC 
GACCCTGGAC TGGTTCTGGC TGGCACCAAG TGTGGAGAGG GCATGATGTG TCTGAATCGT 
CAGTGCCAGA ATGTCAGTGT GTTTGGAGTG CACCAGTGTT CAGGGAAGTG CAGCGGACGA 
GGGGTGTGTA ACAACTTGGA GAACTGCCAT TGTGAGGTGC ACTGGGCCCC TCCCTTCTGT 
GAGAGGGCGG GGTTTGGGGG CAGCCTAGAC AGTGGACCAA TGAGATTGGC AGATGTGCGT 
GTGTGTGTAT TAATGTGCGT GTTGTCGTAT GTGACATCAG CCGACAGCAG GGTGGTTGCT 
GTGGCAATCC TGGCCACCCT CCTCAGCCTT ATGGGTGCTG GGTTCCTGGT ATTCCTTAAG 
AGGAAGACTC TGGTCCGTCT GCTCTTCACA AACAAGAAAA CCACAATGGA GAAACTGAGA 
TCAGTTGGGT CGACCAGACC AGCCAGCCCC AACCAGACTC AGTCCATGTA CAGTCCACGA 
CGCAAGCCCC CAGCCAAGCC TCCAGGTACC AGCATATACA AGCCATCACA CCTGAGTTCG 
GAGCCTCTTC TCCCTCCACA CAGTTTCCAC TCCCACAGCT TGCGGCGGCT GCCCCACTAC 
CAGCCCCTCC ACATCAGCCA CCCCATGCCT GTCTCCCTGA GTGTGGGTCA GCAGCCCTTG 
CCACCGGTCC CGCCCCACCA CAAGGCTCTG CCGGCCCACA CCGCCCTCTC CCCACCACGG 
GCACCGCCCT TCCACAGCCT GCCCCGACAG CAGCCCTACA GAATGGACCA GGACTGCGAG 
AAACCCAGTC CACCCCAGAA GCCTCTGCCT GCAGACCCAC TGGGAAGGAG GGCTCGGGTG 
GGACGCAGCA CCTCGGTGGT GGGGGTTCGC CTCCCTGGAC CCATCCCTAT CCCCATCCCC 
ACCGTACCCA GACCCCTGCT CACCAATCCA CAACTGAACA GACCTGCACC CATACAACCC 
CTGAGACTGC CAAAGACTCA TCATTTTGAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

adam12 ( XM_021611468.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021467143.1 Oncorhynchus mykiss ADAM metallopeptidase domain 12 (adam12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOf52958
Clone ID Related Accession (Same CDS sequence)	XM_021611468.1
Accession Version	XM_021611468.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	disintegrin and metalloproteinase domain-containing protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035077.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAACT GGCGTTGGAC GAACTTTGCA TTTCTAATTG TTTTTCATGT CTTGTCCATG 
TCAACAGCAA CGGGCAATAC AGACAACACA CTTCTGAATT TACATGATGG GAAAAGCTTG 
GACCAGAGTG TGCTGCACAG TGCTGTCCCT AAGCCCCAAC GCTATGACAC CACGGTACCT 
CTCCTGCTGG AAGGGCGGCA GCGCAAACCA CTCAGCCAGC TCAAGTCCAG GAGTCATCCT 
GCATCACTGA AGCTGGTGGT GGAGGCGGAA GGTGAACAAC TGGTCCTGGC ACTAGAAAAA 
AACCAGGGCC TGTTTGCGAG CCATTACACT GAGACACATT ATCTGGACGA TGGCAGTGCA 
GTCACTGGTG CTCACAACTT TACGAAGAAC TGCTACTACC ATGGAGAAGT GGAGGGCCAC 
GCTAACTCTG ACGTCACTCT CAGCACCTGC TCTGGCCTCC GGGGTTTCAT CTCACTTGAA 
AATAAGACCT TCATCCTTGA ACCATCGTTG TACCAGGACA ATAATAAACA CATCATCTAC 
CGAGGGGAAA ACCTCAACCT GACCCCCGGA ACCTGTGGTC ATGGCTTCAA CATGTCCTCT 
GTTACCCCTG ACAACCATCT GAGAAGCCCT TTCAGAACAT TCAGTACCAG GAACAAGAGG 
CATGCTCAGA AAACTACCAA ATATGTAGAG CTGGTCATCG TTGCAGACAA CAGAGAGTTT 
CAGAAACAGG GGAAGGACAT TGAGAGGGTG AAGCAGAGAC TGGCAGAGAT AGCCAATTAC 
GTGGACAAGT TCTATAGGGT TCTGAACATC CGTGTGGCCC TAGTGGGCCT GGAGGTGTGG 
AGCGACGCTG ATAAGTGTCC TGTCACTCAG GACCCCTTCA ACACCCTGCA TGAGTTCCTA 
GACTGGAGGA AAGTCAAACT ACTGCCTACA AAATCACACG ACAACGCTCA GCTCATCAGT 
GGGGTGTACT TCCAGGGTAC CACCATTGGC ATGGCCCCCA TCATGAGCAT GTGTACAGTG 
GAGCAGTCTG GAGGCATCGT CATGGATCAT TCGGATAATC CCCTGGGCGC GGCTGTGACT 
TTAGCACATG AGCTGGGGCA CAACTTTGGC ATGAACCACG ACACTCCAGA GCGGGGGTGT 
GGCTGCAGGA TGACAGTGGA CCGCGGGGGC TGTATCATGA CCCCCTCCAC TGGGTACCCA 
TTTCCTACGG TATTCAGCAC CTGCAGTAAG AAGGACCTGG CAGCCAGTCT GGAGAAGGGA 
GTGGGGATGT GTCTGTACAA CATGCCTGAG GTCAAGGTGC TGTACGGGGG ACAGAAGTGT 
GGCAATGGAT ACGTGGAGGA GGGAGAGGAG TGTGACTGTG GAGAACTGGA GGAATGCATG 
AACCCCTGCT GCAATGCCAC CACCTGCACC CTGAAGGGAA ATGCCGTGTG CGCCCACGGA 
CAGTGCTGCG AGGACTGCAG GCTGAAGCCT GCTGGCACAC CGTGTCGAGA GTCCAGTAAC 
TCCTGTGATT TGCCAGAGTT CTGTACTGGT GCCAATCCCC ACTGCCCTGC CAACGTGTAC 
CTGCATGACG GTCACTCCTG CCACAACGTG GATGGATACT GCTACAACGG CATCTGCCAG 
ACTCATGAGC AACAGTGCAT CACGCTATGG GGACCAGGAG CCAAACCTGC CCCAGGGATC 
TGCTTTGAGA GGGTCAACTC AGCAGGAGAT CCTTACGGGA ACTGTGGGAA GGACTCCAAA 
GGATCGTTTG CCAAATGTGA AGCCCGAGAC GCAAAGTGTG GCAAAATTCA GTGTCAAGGA 
GGGGCCAACC GGCCTGTGAT TGGCACCAAT GCCGTCTCCA TAGAAACCAA CATCCCTCTT 
CAGGAAGGAG GGAGGATCCT GTGTCGGGGA ACCCACGTCT ACCTGGGTGA TGACATGCCC 
GACCCTGGAC TGGTTCTGGC TGGCACCAAG TGTGGAGAGG GCATGATGTG TCTGAATCGT 
CAGTGCCAGA ATGTCAGTGT GTTTGGAGTG CACCAGTGTT CAGGGAAGTG CAGCGGACGA 
GGGGTGTGTA ACAACTTGGA GAACTGCCAT TGTGAGGTGC ACTGGGCCCC TCCCTTCTGT 
GAGAGGGCGG GGTTTGGGGG CAGCCTAGAC AGTGGACCAA TGAGATTGGC AGCCGACAGC 
AGGGTGGTTG CTGTGGCAAT CCTGGCCACC CTCCTCAGCC TTATGGGTGC TGGGTTCCTG 
GTATTCCTTA AGAGGAAGAC TCTGGTCCGT CTGCTCTTCA CAAACAAGAA AACCACAATG 
GAGAAACTGA GATCAGTTGG GTCGACCAGA CCAGCCAGCC CCAACCAGAC TCAGTCCATG 
TACAGTCCAC GACGCAAGCC CCCAGCCAAG CCTCCAGGTA CCAGCATATA CAAGCCATCA 
CACCTGAGTT CGGAGCCTCT TCTCCCTCCA CACAGTTTCC ACTCCCACAG CTTGCGGCGG 
CTGCCCCACT ACCAGCCCCT CCACATCAGC CACCCCATGC CTGTCTCCCT GAGTGTGGGT 
CAGCAGCCCT TGCCACCGGT CCCGCCCCAC CACAAGGCTC TGCCGGCCCA CACCGCCCTC 
TCCCCACCAC GGGCACCGCC CTTCCACAGC CTGCCCCGAC AGCAGCCCTA CAGAATGGAC 
CAGGACTGCG AGAAACCCAG TCCACCCCAG AAGCCTCTGC CTGCAGACCC ACTGGGAAGG 
AGGGCTCGGG TGGGACGCAG CACCTCGGTG GTGGGGGTTC GCCTCCCTGG ACCCATCCCT 
ATCCCCATCC CCACCGTACC CAGACCCCTG CTCACCAATC CACAACTGAA CAGACCTGCA 
CCCATACAAC CCCTGAGACT GCCAAAGACT CATCATTTTG ACCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021467143.1
CDS	454..3321
Translation	MTNWRWTNFAFLIVFHVLSMSTATGNTDNTLLNLHDGKSLDQSVLHSAVPKPQRYDTTVPLLLEGRQRKPLSQLKSRSHPASLKLVVEAEGEQLVLALEKNQGLFASHYTETHYLDDGSAVTGAHNFTKNCYYHGEVEGHANSDVTLSTCSGLRGFISLENKTFILEPSLYQDNNKHIIYRGENLNLTPGTCGHGFNMSSVTPDNHLRSPFRTFSTRNKRHAQKTTKYVELVIVADNREFQKQGKDIERVKQRLAEIANYVDKFYRVLNIRVALVGLEVWSDADKCPVTQDPFNTLHEFLDWRKVKLLPTKSHDNAQLISGVYFQGTTIGMAPIMSMCTVEQSGGIVMDHSDNPLGAAVTLAHELGHNFGMNHDTPERGCGCRMTVDRGGCIMTPSTGYPFPTVFSTCSKKDLAASLEKGVGMCLYNMPEVKVLYGGQKCGNGYVEEGEECDCGELEECMNPCCNATTCTLKGNAVCAHGQCCEDCRLKPAGTPCRESSNSCDLPEFCTGANPHCPANVYLHDGHSCHNVDGYCYNGICQTHEQQCITLWGPGAKPAPGICFERVNSAGDPYGNCGKDSKGSFAKCEARDAKCGKIQCQGGANRPVIGTNAVSIETNIPLQEGGRILCRGTHVYLGDDMPDPGLVLAGTKCGEGMMCLNRQCQNVSVFGVHQCSGKCSGRGVCNNLENCHCEVHWAPPFCERAGFGGSLDSGPMRLAADSRVVAVAILATLLSLMGAGFLVFLKRKTLVRLLFTNKKTTMEKLRSVGSTRPASPNQTQSMYSPRRKPPAKPPGTSIYKPSHLSSEPLLPPHSFHSHSLRRLPHYQPLHISHPMPVSLSVGQQPLPPVPPHHKALPAHTALSPPRAPPFHSLPRQQPYRMDQDCEKPSPPQKPLPADPLGRRARVGRSTSVVGVRLPGPIPIPIPTVPRPLLTNPQLNRPAPIQPLRLPKTHHFDP

Target ORF information:

RefSeq Version	XM_021611468.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss ADAM metallopeptidase domain 12 (adam12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021611468.1

ORF Insert Sequence:

ATGACTAACT GGCGTTGGAC GAACTTTGCA TTTCTAATTG TTTTTCATGT CTTGTCCATG 
TCAACAGCAA CGGGCAATAC AGACAACACA CTTCTGAATT TACATGATGG GAAAAGCTTG 
GACCAGAGTG TGCTGCACAG TGCTGTCCCT AAGCCCCAAC GCTATGACAC CACGGTACCT 
CTCCTGCTGG AAGGGCGGCA GCGCAAACCA CTCAGCCAGC TCAAGTCCAG GAGTCATCCT 
GCATCACTGA AGCTGGTGGT GGAGGCGGAA GGTGAACAAC TGGTCCTGGC ACTAGAAAAA 
AACCAGGGCC TGTTTGCGAG CCATTACACT GAGACACATT ATCTGGACGA TGGCAGTGCA 
GTCACTGGTG CTCACAACTT TACGAAGAAC TGCTACTACC ATGGAGAAGT GGAGGGCCAC 
GCTAACTCTG ACGTCACTCT CAGCACCTGC TCTGGCCTCC GGGGTTTCAT CTCACTTGAA 
AATAAGACCT TCATCCTTGA ACCATCGTTG TACCAGGACA ATAATAAACA CATCATCTAC 
CGAGGGGAAA ACCTCAACCT GACCCCCGGA ACCTGTGGTC ATGGCTTCAA CATGTCCTCT 
GTTACCCCTG ACAACCATCT GAGAAGCCCT TTCAGAACAT TCAGTACCAG GAACAAGAGG 
CATGCTCAGA AAACTACCAA ATATGTAGAG CTGGTCATCG TTGCAGACAA CAGAGAGTTT 
CAGAAACAGG GGAAGGACAT TGAGAGGGTG AAGCAGAGAC TGGCAGAGAT AGCCAATTAC 
GTGGACAAGT TCTATAGGGT TCTGAACATC CGTGTGGCCC TAGTGGGCCT GGAGGTGTGG 
AGCGACGCTG ATAAGTGTCC TGTCACTCAG GACCCCTTCA ACACCCTGCA TGAGTTCCTA 
GACTGGAGGA AAGTCAAACT ACTGCCTACA AAATCACACG ACAACGCTCA GCTCATCAGT 
GGGGTGTACT TCCAGGGTAC CACCATTGGC ATGGCCCCCA TCATGAGCAT GTGTACAGTG 
GAGCAGTCTG GAGGCATCGT CATGGATCAT TCGGATAATC CCCTGGGCGC GGCTGTGACT 
TTAGCACATG AGCTGGGGCA CAACTTTGGC ATGAACCACG ACACTCCAGA GCGGGGGTGT 
GGCTGCAGGA TGACAGTGGA CCGCGGGGGC TGTATCATGA CCCCCTCCAC TGGGTACCCA 
TTTCCTACGG TATTCAGCAC CTGCAGTAAG AAGGACCTGG CAGCCAGTCT GGAGAAGGGA 
GTGGGGATGT GTCTGTACAA CATGCCTGAG GTCAAGGTGC TGTACGGGGG ACAGAAGTGT 
GGCAATGGAT ACGTGGAGGA GGGAGAGGAG TGTGACTGTG GAGAACTGGA GGAATGCATG 
AACCCCTGCT GCAATGCCAC CACCTGCACC CTGAAGGGAA ATGCCGTGTG CGCCCACGGA 
CAGTGCTGCG AGGACTGCAG GCTGAAGCCT GCTGGCACAC CGTGTCGAGA GTCCAGTAAC 
TCCTGTGATT TGCCAGAGTT CTGTACTGGT GCCAATCCCC ACTGCCCTGC CAACGTGTAC 
CTGCATGACG GTCACTCCTG CCACAACGTG GATGGATACT GCTACAACGG CATCTGCCAG 
ACTCATGAGC AACAGTGCAT CACGCTATGG GGACCAGGAG CCAAACCTGC CCCAGGGATC 
TGCTTTGAGA GGGTCAACTC AGCAGGAGAT CCTTACGGGA ACTGTGGGAA GGACTCCAAA 
GGATCGTTTG CCAAATGTGA AGCCCGAGAC GCAAAGTGTG GCAAAATTCA GTGTCAAGGA 
GGGGCCAACC GGCCTGTGAT TGGCACCAAT GCCGTCTCCA TAGAAACCAA CATCCCTCTT 
CAGGAAGGAG GGAGGATCCT GTGTCGGGGA ACCCACGTCT ACCTGGGTGA TGACATGCCC 
GACCCTGGAC TGGTTCTGGC TGGCACCAAG TGTGGAGAGG GCATGATGTG TCTGAATCGT 
CAGTGCCAGA ATGTCAGTGT GTTTGGAGTG CACCAGTGTT CAGGGAAGTG CAGCGGACGA 
GGGGTGTGTA ACAACTTGGA GAACTGCCAT TGTGAGGTGC ACTGGGCCCC TCCCTTCTGT 
GAGAGGGCGG GGTTTGGGGG CAGCCTAGAC AGTGGACCAA TGAGATTGGC AGCCGACAGC 
AGGGTGGTTG CTGTGGCAAT CCTGGCCACC CTCCTCAGCC TTATGGGTGC TGGGTTCCTG 
GTATTCCTTA AGAGGAAGAC TCTGGTCCGT CTGCTCTTCA CAAACAAGAA AACCACAATG 
GAGAAACTGA GATCAGTTGG GTCGACCAGA CCAGCCAGCC CCAACCAGAC TCAGTCCATG 
TACAGTCCAC GACGCAAGCC CCCAGCCAAG CCTCCAGGTA CCAGCATATA CAAGCCATCA 
CACCTGAGTT CGGAGCCTCT TCTCCCTCCA CACAGTTTCC ACTCCCACAG CTTGCGGCGG 
CTGCCCCACT ACCAGCCCCT CCACATCAGC CACCCCATGC CTGTCTCCCT GAGTGTGGGT 
CAGCAGCCCT TGCCACCGGT CCCGCCCCAC CACAAGGCTC TGCCGGCCCA CACCGCCCTC 
TCCCCACCAC GGGCACCGCC CTTCCACAGC CTGCCCCGAC AGCAGCCCTA CAGAATGGAC 
CAGGACTGCG AGAAACCCAG TCCACCCCAG AAGCCTCTGC CTGCAGACCC ACTGGGAAGG 
AGGGCTCGGG TGGGACGCAG CACCTCGGTG GTGGGGGTTC GCCTCCCTGG ACCCATCCCT 
ATCCCCATCC CCACCGTACC CAGACCCCTG CTCACCAATC CACAACTGAA CAGACCTGCA 
CCCATACAAC CCCTGAGACT GCCAAAGACT CATCATTTTG ACCCCTGA