ddx43 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddx43
Entrez Gene ID	110528523
Full Name	DEAD-box helicase 43
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021610639.1	XP_021466314.1	probable ATP-dependent RNA helicase DDX43

Pathways from BioSystems
Gene Ontology

The following ddx43 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddx43 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf52869	XM_021610639.1 Latest version!	Oncorhynchus mykiss DEAD-box helicase 43 (ddx43), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddx43 ( XM_021610639.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021466314.1 Oncorhynchus mykiss DEAD-box helicase 43 (ddx43), mRNA.

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CloneID	OOf52869
Clone ID Related Accession (Same CDS sequence)	XM_021610639.1
Accession Version	XM_021610639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	probable ATP-dependent RNA helicase DDX43
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACT GGGAGGAGGA AAACGATGTA CCTACGCCTG CTACCACAGC ACCACCCACA 
GATTGGAGCT CTTCGTGGAA AGGTGGTGCG AAGGAAAATG TGTGTTTTGG GATGGGAAAT 
GCAGGTGGTT TCAGAGCGCC AAGAGAGAGA GGGGATCCCA ACGGTTCGCA TTGTCAAAAT 
AGGAGGGACA GCTATGGTGG CAACAACCGC GTGTTCTCCA GAAGTGGAGA TATTTATGCC 
TGTGGGCGCA GAGGCCGTGG CGCAGACGGG TCGACTTCAG CTCCGCCCGT CACCTTCACG 
GTGGAAAATA CCTCAATTGG AAGGGTTATA GGTCGCGGTG GAGCTAAAAT ACGTGAACTT 
GAAGAAACCA GTGGTGCACG AATCAAGATC AGCAAGGGGG ACTATGAGGG TGAAGTGCAT 
ATCTTCGGGA GCAGCAAGGT TCAACAGAAG GCCCGAGAGA TGATCGAGGA GTTGGTAGCC 
GGAGACAATG GCCCTGGGGG ATTCCGCAGA GAACCTGGGT GTGATGGAAG ATTTGGGGGT 
GCTGGTGGCG GAGGCAGGAG CGGCTCCATC TGGTCAGCTG CAGCCCTGCA GGCGTCTCTG 
GAGGACAGTC CTCCACAGGC CATCGACTGG GTCTCCATGA GGGCCAACCG GCACAAATAC 
GAGGAGATGA AGTGGCAGGA TCTCCCATCT CTGAAGAAGG ATTTCTACTT TGAGTCAGAG 
ATTGTAGCCA GTCGGTGTGA AGAGGAAGTA AAGGCATGGA GGAAAGTGAA TAACGATATC 
TTTGTGGATG ACCTGAAGGA GGGAGAAGGG GAGAAGCGTC CTTTTCCCAA CCCTGTGGTC 
ACGTTCGACG AAGCCTTCCT GCACTATCCT GGGATCATGG AGAATATTGT CAGAGTCGGC 
TTCGCCAAGC CAACTCCTAT CCAGTCTCAA GCCTGGCCCA TGTCGCTGAA AGGAGATGAC 
CTGATCGCGA TCGCCCAGAC AGGCACAGGA AAGACACTGG CCTACCTCCT ACCTGGCTTC 
ATTCACATGG ATGGCCAGCC TGTAAAACGT GATAAGCAGG ACGGCCCTGG TATGCTTGTT 
CTGACTCCCA CTAGAGAGCT GGCTCTGCAG ATTGAGTCAG AGTGCAACAA GTACAGCTAC 
AAGGGCTTCA AATGTGTGTG TGTCTACGGA GGTGGAGACC GCAGGGCCCA GATCAAAGTG 
ATCTCAGCTG GAGTAGACAT TGTGATCGCT ACGCCTGGCC GGCTTAACGA CCTGCAGATG 
AACGAGTTGA TCAACCTGCA TTCCATCACC TACCTGGTGC TGGATGAGGC AGACCGGATG 
CTTGACATGG GCTTTGAGCC TCAAATAATG AAAATTCTTC TGGACGTCCG TCCGGACAGG 
CAGACTATTA TGACCAGTGC CACCTGGCCC ACGGGAGTGA GGCGAATGGC CAAGACGTAC 
CTGAAGAACC CCATGATGGT GTATGTGGGA ACTCTGGACC TGGCTGCTGT GAACACAGTG 
CAGCAGACTG TTCTCTTTTG TCAAGACGAG GAGAAGAAGG CCTATCTCTT TAACTTCGTC 
CACAACATGG AGCCTGAGGA CAAAGTGCTC ATCTTCGTAG GCAAGAAGAT AATGTGTGAC 
GACCTGTCCA GTGACATGTG TCTGCGGGGC CTGGCAGTAC AGAGTCTTCA TGGGGACCGG 
GAACAGTGTG ACCGCGAGGA GGCGCTCAAG GACTTCAAAG AAAGTGTTGT CCGTATCCTA 
GTGGCCACTG ACCTGGCCTC CCGAGGGCTG GACGTCACAG ACGTCACACA CGTCTTCAAC 
TTTGACTTCC CACGCAACAT TGAAGAGTAT GTCCATCGCG TGGGCCGCAC GGGCCGGGCA 
GGACGCTCTG GGGCCTCTAT CACCTTGGTA ACGAGAGGAG ACTGGAGGAA GGCTGCTGAC 
CTGATCCCCA TCCTGGAGAG GGCTGGACAG GAGGTACCTG ATGAGCTGGT GCTCATGGCG 
GAGAGATACG AGAAGCACAA GCGAGAGAAG ATGATGTACG GCCCCAGAGG AGGAGGAGGG 
GGAGGAGGAG TAAGAGACAG AGGAGAGTCT AACTGGAGGT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021466314.1
CDS	205..2289
Translation	MSDWEEENDVPTPATTAPPTDWSSSWKGGAKENVCFGMGNAGGFRAPRERGDPNGSHCQNRRDSYGGNNRVFSRSGDIYACGRRGRGADGSTSAPPVTFTVENTSIGRVIGRGGAKIRELEETSGARIKISKGDYEGEVHIFGSSKVQQKAREMIEELVAGDNGPGGFRREPGCDGRFGGAGGGGRSGSIWSAAALQASLEDSPPQAIDWVSMRANRHKYEEMKWQDLPSLKKDFYFESEIVASRCEEEVKAWRKVNNDIFVDDLKEGEGEKRPFPNPVVTFDEAFLHYPGIMENIVRVGFAKPTPIQSQAWPMSLKGDDLIAIAQTGTGKTLAYLLPGFIHMDGQPVKRDKQDGPGMLVLTPTRELALQIESECNKYSYKGFKCVCVYGGGDRRAQIKVISAGVDIVIATPGRLNDLQMNELINLHSITYLVLDEADRMLDMGFEPQIMKILLDVRPDRQTIMTSATWPTGVRRMAKTYLKNPMMVYVGTLDLAAVNTVQQTVLFCQDEEKKAYLFNFVHNMEPEDKVLIFVGKKIMCDDLSSDMCLRGLAVQSLHGDREQCDREEALKDFKESVVRILVATDLASRGLDVTDVTHVFNFDFPRNIEEYVHRVGRTGRAGRSGASITLVTRGDWRKAADLIPILERAGQEVPDELVLMAERYEKHKREKMMYGPRGGGGGGGVRDRGESNWRF

Target ORF information:

RefSeq Version	XM_021610639.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss DEAD-box helicase 43 (ddx43), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021610639.1

ORF Insert Sequence:

ATGTCGGACT GGGAGGAGGA AAACGATGTA CCTACGCCTG CTACCACAGC ACCACCCACA 
GATTGGAGCT CTTCGTGGAA AGGTGGTGCG AAGGAAAATG TGTGTTTTGG GATGGGAAAT 
GCAGGTGGTT TCAGAGCGCC AAGAGAGAGA GGGGATCCCA ACGGTTCGCA TTGTCAAAAT 
AGGAGGGACA GCTATGGTGG CAACAACCGC GTGTTCTCCA GAAGTGGAGA TATTTATGCC 
TGTGGGCGCA GAGGCCGTGG CGCAGACGGG TCGACTTCAG CTCCGCCCGT CACCTTCACG 
GTGGAAAATA CCTCAATTGG AAGGGTTATA GGTCGCGGTG GAGCTAAAAT ACGTGAACTT 
GAAGAAACCA GTGGTGCACG AATCAAGATC AGCAAGGGGG ACTATGAGGG TGAAGTGCAT 
ATCTTCGGGA GCAGCAAGGT TCAACAGAAG GCCCGAGAGA TGATCGAGGA GTTGGTAGCC 
GGAGACAATG GCCCTGGGGG ATTCCGCAGA GAACCTGGGT GTGATGGAAG ATTTGGGGGT 
GCTGGTGGCG GAGGCAGGAG CGGCTCCATC TGGTCAGCTG CAGCCCTGCA GGCGTCTCTG 
GAGGACAGTC CTCCACAGGC CATCGACTGG GTCTCCATGA GGGCCAACCG GCACAAATAC 
GAGGAGATGA AGTGGCAGGA TCTCCCATCT CTGAAGAAGG ATTTCTACTT TGAGTCAGAG 
ATTGTAGCCA GTCGGTGTGA AGAGGAAGTA AAGGCATGGA GGAAAGTGAA TAACGATATC 
TTTGTGGATG ACCTGAAGGA GGGAGAAGGG GAGAAGCGTC CTTTTCCCAA CCCTGTGGTC 
ACGTTCGACG AAGCCTTCCT GCACTATCCT GGGATCATGG AGAATATTGT CAGAGTCGGC 
TTCGCCAAGC CAACTCCTAT CCAGTCTCAA GCCTGGCCCA TGTCGCTGAA AGGAGATGAC 
CTGATCGCGA TCGCCCAGAC AGGCACAGGA AAGACACTGG CCTACCTCCT ACCTGGCTTC 
ATTCACATGG ATGGCCAGCC TGTAAAACGT GATAAGCAGG ACGGCCCTGG TATGCTTGTT 
CTGACTCCCA CTAGAGAGCT GGCTCTGCAG ATTGAGTCAG AGTGCAACAA GTACAGCTAC 
AAGGGCTTCA AATGTGTGTG TGTCTACGGA GGTGGAGACC GCAGGGCCCA GATCAAAGTG 
ATCTCAGCTG GAGTAGACAT TGTGATCGCT ACGCCTGGCC GGCTTAACGA CCTGCAGATG 
AACGAGTTGA TCAACCTGCA TTCCATCACC TACCTGGTGC TGGATGAGGC AGACCGGATG 
CTTGACATGG GCTTTGAGCC TCAAATAATG AAAATTCTTC TGGACGTCCG TCCGGACAGG 
CAGACTATTA TGACCAGTGC CACCTGGCCC ACGGGAGTGA GGCGAATGGC CAAGACGTAC 
CTGAAGAACC CCATGATGGT GTATGTGGGA ACTCTGGACC TGGCTGCTGT GAACACAGTG 
CAGCAGACTG TTCTCTTTTG TCAAGACGAG GAGAAGAAGG CCTATCTCTT TAACTTCGTC 
CACAACATGG AGCCTGAGGA CAAAGTGCTC ATCTTCGTAG GCAAGAAGAT AATGTGTGAC 
GACCTGTCCA GTGACATGTG TCTGCGGGGC CTGGCAGTAC AGAGTCTTCA TGGGGACCGG 
GAACAGTGTG ACCGCGAGGA GGCGCTCAAG GACTTCAAAG AAAGTGTTGT CCGTATCCTA 
GTGGCCACTG ACCTGGCCTC CCGAGGGCTG GACGTCACAG ACGTCACACA CGTCTTCAAC 
TTTGACTTCC CACGCAACAT TGAAGAGTAT GTCCATCGCG TGGGCCGCAC GGGCCGGGCA 
GGACGCTCTG GGGCCTCTAT CACCTTGGTA ACGAGAGGAG ACTGGAGGAA GGCTGCTGAC 
CTGATCCCCA TCCTGGAGAG GGCTGGACAG GAGGTACCTG ATGAGCTGGT GCTCATGGCG 
GAGAGATACG AGAAGCACAA GCGAGAGAAG ATGATGTACG GCCCCAGAGG AGGAGGAGGG 
GGAGGAGGAG TAAGAGACAG AGGAGAGTCT AACTGGAGGT TCTAA