nvl cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	nvl
Entrez Gene ID	110528362
Full Name	nuclear VCP-like
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021610378.1	XP_021466053.1	nuclear valosin-containing protein-like isoform X1
XM_021610389.1	XP_021466064.1	nuclear valosin-containing protein-like isoform X2

Pathways from BioSystems
Gene Ontology

The following nvl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nvl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf52845	XM_021610378.1 Latest version!	Oncorhynchus mykiss nuclear VCP-like (nvl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OOf52846	XM_021610389.1 Latest version!	Oncorhynchus mykiss nuclear VCP-like (nvl), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nvl ( XM_021610378.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021466053.1 Oncorhynchus mykiss nuclear VCP-like (nvl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOf52845
Clone ID Related Accession (Same CDS sequence)	XM_021610378.1
Accession Version	XM_021610378.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	nuclear valosin-containing protein-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAACA GACGTTGCGG AGTGGATAAC CGACTCAAAC AGAGAGTTGA GCAGTATTTG 
AGTAACCAGA GTGGCCAGTA TGTTGACATT ACTTCAATGG CACATGATCT TCAGAGACTC 
TACAGAATGG ACTATGGCAG GCGAAACAAG ACTGCATTCA GGATTCAGGT TGAAAAAGTC 
CATGGAGTGA TATGCGATGA ATCTGGACTG AATGATCTGG AGAACAAGCA CTTGGCCAAG 
AGAGCCAGAC ACAGTGAGAA GGATGCTGGG GATGGCAGCA GTAACTTGGA TGACACTACT 
GATAGTGACG AGGACCTTCC TGAACAACCG CCCATCAACC TCATGAATAA CTCCCTGATG 
TCCCTGTACA AGAAGGGGAA CCCAGAGAGT GGGGCATCTC CCAGGAGGGA CAAGTCTGCA 
TCAGACAGCC CTGCCCCTGA GGCTCACTCC ACCCCTCAGA GTGTTGGTAC CAAGGTGTCT 
GCAGGGGGCT GGTTCATAGA CAAGGGCAGA GGCCTGGAGC ATGACAGCAT CCTCATTGAC 
CTGTGTGAAG AGGAGGCAGC CAGTCCTGCA GGAAATAAGC AGACAGATGC CTCAATGCTT 
GAGCCTGAGA AGACACCAAA AAAAGGCACA TTTAAAAAAT CTAAACGGCG GAAAGAGGGA 
GCGTCAAAGG GCAATGATGG TGACATCGAG GCCAGTATCA TGAACAAGAA AGCGAGGACC 
AAATCCATTG AACTACAGTA TTCAACATTA AAGTTTGAAG ATGTTGGAGG CAATGAGGAC 
ACATTGACGG AGGTGTGTAA GCTGCTGATA CATATGCGTC ACCCAGAGGT GTACCAGCAG 
TTGGGGGTAG TCCCTCCACG GGGGTTCCTG CTCCATGGCC CCCCTGGCTG TGGAAAAACC 
CTGCTGGCCC AAGCTGTGGC TGGGGAAATG GAGCTGCCCA TGCTGAAGGT ATCGGCACCA 
GAGCTGGTGT CTGGGGTGTC AGGGGAGTCT GAGCAGAAGC TGAGAGAGCT GTTTGAGCAG 
GCTGTGACCA GCTCCCCTTG CATCCTGTTC ATTGATGAGA TTGATGCCAT CACTCCTAAG 
CGAGAGGTGG CCTCCAAGGA CATGGAGAGA CGGATTGTTG CTCAGCTGCT CACCTGCATG 
GATGACCTGA ACTCATTAGC GGTCACAGCC CAGGTGATGG TTATTGGTGC CACCAACCGG 
CCAGACTCTC TGGACCCAGC GCTGCGCCGG GCTGGACGCT TTGACAAAGA AATCTGTCTG 
GGGATACCTG ATGAAGCAGC TCGTCTCAGA ATTCTGAAGA CCCTGTGTCG GAAGCTGAAG 
CTCCCTGAGG ACTTTGACTA CCGGCAGCTG GCGCGTCTTA CCCCGGGGTA CGTGGGAGCT 
GACCTCATGG CGCTGTGCCG CGAGGCCGCC ATGAGCGCCG TCAACCGGGT CCTGCTGGAG 
AGGCAGGACA AGAACCAGGG GCTGGTCCCC ATGGCGGAGG AGAGAGCAGA GTCTGAGGGG 
CTTCAGGGCA CAGGTCCTCT CCCACAGGGC CCAGAGAGAT TCCAGGCCTC AGCAGACACA 
GTGATCCAGG AGATGACAGA CCGTAGCCAC GTACCACAGG GAGACAAGCA GCAGCTGCCT 
CTGGCTACTG AGACAACCTT CAGGGGGGAG CTTTGTCGCC TGCTGTCCCT GCTGAAGAGC 
AGTGAGTCGC TGTCTGAGGA GCAGCTTGCT GGCTTGTGCA TCCTCATGTC AGACTTCCAG 
GGTTCCCTGG CCAGCGTACA ACCCTCTGCA AAGCGTGAAG GCTTCGCCAC GGTGCCAGAC 
GTCACCTGGG AGGACGTGGG GGCCCTGCAT GACATCCGCG AAGAGCTCAC CATGGCCATC 
CTGGCCCCTG TGCGTTCTCC TGAGCAGTTC AAAGCTCTGG GTCTCAGTGC TCCTGCAGGG 
GTGTTGCTAG CTGGGCCTCC CGGTTGTGGA AAGACCCTGC TGGCCAAGGC TGTTGCCAAT 
GAGTCAGGAC TTAACTTCAT CTCTGTCAAG GGGCCAGAGC TGCTGAACAT GTATGTGGGA 
GAGAGCGAGC GGGCCGTGCG ACAGGTGTTC CAGAGAGGAC GGAACTCTGC CCCTTGTGTC 
ATCTTTTTCG ACGAGATCGA TGCACTGTGC CCCCGCCGCT CCGGGCATGA GTCTGGAGCC 
AGTGTCCGTG TGGTCAACCA GCTGCTTACT GAGATGGATG GACTGGAGAC CCGGCGGCAG 
GTCTTCATCA TGGCGGCTAC CAATAGACCA GATATCATTG ATCCTGCTGT GCTGAGACCA 
GGTCGTCTGG ACAAGACCCT GTATGTGGGC CTCCCACCTC CATCAGACCG GCACGCCATC 
TTACTCACCA TCACCAAGGG GGGCAGCAGG CCTCATCTGG AGCAGGATGT GAGTCTGGAG 
GAGATGGCCC ATGACGTGCG CAGCGACTGC TTCACTGGGG CAGACCTGTC TGCATTGGTG 
AGGGAAGCCT CTGTCAATGC TCTGAGAGCC TACCTCCTGA CCCAACCCAA CCCATCTTAC 
ACAGGTCACA GCTACTCCAA CGGCCCTGTA GCCGACATCC GGGTCAGCAA GCAGAACTTT 
GAGGACGCCT TCAAGAAAGT ACGGCCGTCT GTGTCTAAAA AGGACCAGCG TATGTACGAG 
CAGCTAAAGG AATCTCTTAC AAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021466053.1
CDS	166..2832
Translation	MNNRRCGVDNRLKQRVEQYLSNQSGQYVDITSMAHDLQRLYRMDYGRRNKTAFRIQVEKVHGVICDESGLNDLENKHLAKRARHSEKDAGDGSSNLDDTTDSDEDLPEQPPINLMNNSLMSLYKKGNPESGASPRRDKSASDSPAPEAHSTPQSVGTKVSAGGWFIDKGRGLEHDSILIDLCEEEAASPAGNKQTDASMLEPEKTPKKGTFKKSKRRKEGASKGNDGDIEASIMNKKARTKSIELQYSTLKFEDVGGNEDTLTEVCKLLIHMRHPEVYQQLGVVPPRGFLLHGPPGCGKTLLAQAVAGEMELPMLKVSAPELVSGVSGESEQKLRELFEQAVTSSPCILFIDEIDAITPKREVASKDMERRIVAQLLTCMDDLNSLAVTAQVMVIGATNRPDSLDPALRRAGRFDKEICLGIPDEAARLRILKTLCRKLKLPEDFDYRQLARLTPGYVGADLMALCREAAMSAVNRVLLERQDKNQGLVPMAEERAESEGLQGTGPLPQGPERFQASADTVIQEMTDRSHVPQGDKQQLPLATETTFRGELCRLLSLLKSSESLSEEQLAGLCILMSDFQGSLASVQPSAKREGFATVPDVTWEDVGALHDIREELTMAILAPVRSPEQFKALGLSAPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRGRNSAPCVIFFDEIDALCPRRSGHESGASVRVVNQLLTEMDGLETRRQVFIMAATNRPDIIDPAVLRPGRLDKTLYVGLPPPSDRHAILLTITKGGSRPHLEQDVSLEEMAHDVRSDCFTGADLSALVREASVNALRAYLLTQPNPSYTGHSYSNGPVADIRVSKQNFEDAFKKVRPSVSKKDQRMYEQLKESLTR

Target ORF information:

RefSeq Version	XM_021610378.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss nuclear VCP-like (nvl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021610378.1

ORF Insert Sequence:

ATGAATAACA GACGTTGCGG AGTGGATAAC CGACTCAAAC AGAGAGTTGA GCAGTATTTG 
AGTAACCAGA GTGGCCAGTA TGTTGACATT ACTTCAATGG CACATGATCT TCAGAGACTC 
TACAGAATGG ACTATGGCAG GCGAAACAAG ACTGCATTCA GGATTCAGGT TGAAAAAGTC 
CATGGAGTGA TATGCGATGA ATCTGGACTG AATGATCTGG AGAACAAGCA CTTGGCCAAG 
AGAGCCAGAC ACAGTGAGAA GGATGCTGGG GATGGCAGCA GTAACTTGGA TGACACTACT 
GATAGTGACG AGGACCTTCC TGAACAACCG CCCATCAACC TCATGAATAA CTCCCTGATG 
TCCCTGTACA AGAAGGGGAA CCCAGAGAGT GGGGCATCTC CCAGGAGGGA CAAGTCTGCA 
TCAGACAGCC CTGCCCCTGA GGCTCACTCC ACCCCTCAGA GTGTTGGTAC CAAGGTGTCT 
GCAGGGGGCT GGTTCATAGA CAAGGGCAGA GGCCTGGAGC ATGACAGCAT CCTCATTGAC 
CTGTGTGAAG AGGAGGCAGC CAGTCCTGCA GGAAATAAGC AGACAGATGC CTCAATGCTT 
GAGCCTGAGA AGACACCAAA AAAAGGCACA TTTAAAAAAT CTAAACGGCG GAAAGAGGGA 
GCGTCAAAGG GCAATGATGG TGACATCGAG GCCAGTATCA TGAACAAGAA AGCGAGGACC 
AAATCCATTG AACTACAGTA TTCAACATTA AAGTTTGAAG ATGTTGGAGG CAATGAGGAC 
ACATTGACGG AGGTGTGTAA GCTGCTGATA CATATGCGTC ACCCAGAGGT GTACCAGCAG 
TTGGGGGTAG TCCCTCCACG GGGGTTCCTG CTCCATGGCC CCCCTGGCTG TGGAAAAACC 
CTGCTGGCCC AAGCTGTGGC TGGGGAAATG GAGCTGCCCA TGCTGAAGGT ATCGGCACCA 
GAGCTGGTGT CTGGGGTGTC AGGGGAGTCT GAGCAGAAGC TGAGAGAGCT GTTTGAGCAG 
GCTGTGACCA GCTCCCCTTG CATCCTGTTC ATTGATGAGA TTGATGCCAT CACTCCTAAG 
CGAGAGGTGG CCTCCAAGGA CATGGAGAGA CGGATTGTTG CTCAGCTGCT CACCTGCATG 
GATGACCTGA ACTCATTAGC GGTCACAGCC CAGGTGATGG TTATTGGTGC CACCAACCGG 
CCAGACTCTC TGGACCCAGC GCTGCGCCGG GCTGGACGCT TTGACAAAGA AATCTGTCTG 
GGGATACCTG ATGAAGCAGC TCGTCTCAGA ATTCTGAAGA CCCTGTGTCG GAAGCTGAAG 
CTCCCTGAGG ACTTTGACTA CCGGCAGCTG GCGCGTCTTA CCCCGGGGTA CGTGGGAGCT 
GACCTCATGG CGCTGTGCCG CGAGGCCGCC ATGAGCGCCG TCAACCGGGT CCTGCTGGAG 
AGGCAGGACA AGAACCAGGG GCTGGTCCCC ATGGCGGAGG AGAGAGCAGA GTCTGAGGGG 
CTTCAGGGCA CAGGTCCTCT CCCACAGGGC CCAGAGAGAT TCCAGGCCTC AGCAGACACA 
GTGATCCAGG AGATGACAGA CCGTAGCCAC GTACCACAGG GAGACAAGCA GCAGCTGCCT 
CTGGCTACTG AGACAACCTT CAGGGGGGAG CTTTGTCGCC TGCTGTCCCT GCTGAAGAGC 
AGTGAGTCGC TGTCTGAGGA GCAGCTTGCT GGCTTGTGCA TCCTCATGTC AGACTTCCAG 
GGTTCCCTGG CCAGCGTACA ACCCTCTGCA AAGCGTGAAG GCTTCGCCAC GGTGCCAGAC 
GTCACCTGGG AGGACGTGGG GGCCCTGCAT GACATCCGCG AAGAGCTCAC CATGGCCATC 
CTGGCCCCTG TGCGTTCTCC TGAGCAGTTC AAAGCTCTGG GTCTCAGTGC TCCTGCAGGG 
GTGTTGCTAG CTGGGCCTCC CGGTTGTGGA AAGACCCTGC TGGCCAAGGC TGTTGCCAAT 
GAGTCAGGAC TTAACTTCAT CTCTGTCAAG GGGCCAGAGC TGCTGAACAT GTATGTGGGA 
GAGAGCGAGC GGGCCGTGCG ACAGGTGTTC CAGAGAGGAC GGAACTCTGC CCCTTGTGTC 
ATCTTTTTCG ACGAGATCGA TGCACTGTGC CCCCGCCGCT CCGGGCATGA GTCTGGAGCC 
AGTGTCCGTG TGGTCAACCA GCTGCTTACT GAGATGGATG GACTGGAGAC CCGGCGGCAG 
GTCTTCATCA TGGCGGCTAC CAATAGACCA GATATCATTG ATCCTGCTGT GCTGAGACCA 
GGTCGTCTGG ACAAGACCCT GTATGTGGGC CTCCCACCTC CATCAGACCG GCACGCCATC 
TTACTCACCA TCACCAAGGG GGGCAGCAGG CCTCATCTGG AGCAGGATGT GAGTCTGGAG 
GAGATGGCCC ATGACGTGCG CAGCGACTGC TTCACTGGGG CAGACCTGTC TGCATTGGTG 
AGGGAAGCCT CTGTCAATGC TCTGAGAGCC TACCTCCTGA CCCAACCCAA CCCATCTTAC 
ACAGGTCACA GCTACTCCAA CGGCCCTGTA GCCGACATCC GGGTCAGCAA GCAGAACTTT 
GAGGACGCCT TCAAGAAAGT ACGGCCGTCT GTGTCTAAAA AGGACCAGCG TATGTACGAG 
CAGCTAAAGG AATCTCTTAC AAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nvl ( XM_021610389.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021466064.1 Oncorhynchus mykiss nuclear VCP-like (nvl), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOf52846
Clone ID Related Accession (Same CDS sequence)	XM_021610389.1
Accession Version	XM_021610389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	nuclear valosin-containing protein-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTATG GCAGGCGAAA CAAGACTGCA TTCAGGATTC AGGTTGAAAA AGTCCATGGA 
GTGATATGCG ATGAATCTGG ACTGAATGAT CTGGAGAACA AGCACTTGGC CAAGAGAGCC 
AGACACAGTG AGAAGGATGC TGGGGATGGC AGCAGTAACT TGGATGACAC TACTGATAGT 
GACGAGGACC TTCCTGAACA ACCGCCCATC AACCTCATGA ATAACTCCCT GATGTCCCTG 
TACAAGAAGG GGAACCCAGA GAGTGGGGCA TCTCCCAGGA GGGACAAGTC TGCATCAGAC 
AGCCCTGCCC CTGAGGCTCA CTCCACCCCT CAGAGTGTTG GTACCAAGGT GTCTGCAGGG 
GGCTGGTTCA TAGACAAGGG CAGAGGCCTG GAGCATGACA GCATCCTCAT TGACCTGTGT 
GAAGAGGAGG CAGCCAGTCC TGCAGGAAAT AAGCAGACAG ATGCCTCAAT GCTTGAGCCT 
GAGAAGACAC CAAAAAAAGG CACATTTAAA AAATCTAAAC GGCGGAAAGA GGGAGCGTCA 
AAGGGCAATG ATGGTGACAT CGAGGCCAGT ATCATGAACA AGAAAGCGAG GACCAAATCC 
ATTGAACTAC AGTATTCAAC ATTAAAGTTT GAAGATGTTG GAGGCAATGA GGACACATTG 
ACGGAGGTGT GTAAGCTGCT GATACATATG CGTCACCCAG AGGTGTACCA GCAGTTGGGG 
GTAGTCCCTC CACGGGGGTT CCTGCTCCAT GGCCCCCCTG GCTGTGGAAA AACCCTGCTG 
GCCCAAGCTG TGGCTGGGGA AATGGAGCTG CCCATGCTGA AGGTATCGGC ACCAGAGCTG 
GTGTCTGGGG TGTCAGGGGA GTCTGAGCAG AAGCTGAGAG AGCTGTTTGA GCAGGCTGTG 
ACCAGCTCCC CTTGCATCCT GTTCATTGAT GAGATTGATG CCATCACTCC TAAGCGAGAG 
GTGGCCTCCA AGGACATGGA GAGACGGATT GTTGCTCAGC TGCTCACCTG CATGGATGAC 
CTGAACTCAT TAGCGGTCAC AGCCCAGGTG ATGGTTATTG GTGCCACCAA CCGGCCAGAC 
TCTCTGGACC CAGCGCTGCG CCGGGCTGGA CGCTTTGACA AAGAAATCTG TCTGGGGATA 
CCTGATGAAG CAGCTCGTCT CAGAATTCTG AAGACCCTGT GTCGGAAGCT GAAGCTCCCT 
GAGGACTTTG ACTACCGGCA GCTGGCGCGT CTTACCCCGG GGTACGTGGG AGCTGACCTC 
ATGGCGCTGT GCCGCGAGGC CGCCATGAGC GCCGTCAACC GGGTCCTGCT GGAGAGGCAG 
GACAAGAACC AGGGGCTGGT CCCCATGGCG GAGGAGAGAG CAGAGTCTGA GGGGCTTCAG 
GGCACAGGTC CTCTCCCACA GGGCCCAGAG AGATTCCAGG CCTCAGCAGA CACAGTGATC 
CAGGAGATGA CAGACCGTAG CCACGTACCA CAGGGAGACA AGCAGCAGCT GCCTCTGGCT 
ACTGAGACAA CCTTCAGGGG GGAGCTTTGT CGCCTGCTGT CCCTGCTGAA GAGCAGTGAG 
TCGCTGTCTG AGGAGCAGCT TGCTGGCTTG TGCATCCTCA TGTCAGACTT CCAGGGTTCC 
CTGGCCAGCG TACAACCCTC TGCAAAGCGT GAAGGCTTCG CCACGGTGCC AGACGTCACC 
TGGGAGGACG TGGGGGCCCT GCATGACATC CGCGAAGAGC TCACCATGGC CATCCTGGCC 
CCTGTGCGTT CTCCTGAGCA GTTCAAAGCT CTGGGTCTCA GTGCTCCTGC AGGGGTGTTG 
CTAGCTGGGC CTCCCGGTTG TGGAAAGACC CTGCTGGCCA AGGCTGTTGC CAATGAGTCA 
GGACTTAACT TCATCTCTGT CAAGGGGCCA GAGCTGCTGA ACATGTATGT GGGAGAGAGC 
GAGCGGGCCG TGCGACAGGT GTTCCAGAGA GGACGGAACT CTGCCCCTTG TGTCATCTTT 
TTCGACGAGA TCGATGCACT GTGCCCCCGC CGCTCCGGGC ATGAGTCTGG AGCCAGTGTC 
CGTGTGGTCA ACCAGCTGCT TACTGAGATG GATGGACTGG AGACCCGGCG GCAGGTCTTC 
ATCATGGCGG CTACCAATAG ACCAGATATC ATTGATCCTG CTGTGCTGAG ACCAGGTCGT 
CTGGACAAGA CCCTGTATGT GGGCCTCCCA CCTCCATCAG ACCGGCACGC CATCTTACTC 
ACCATCACCA AGGGGGGCAG CAGGCCTCAT CTGGAGCAGG ATGTGAGTCT GGAGGAGATG 
GCCCATGACG TGCGCAGCGA CTGCTTCACT GGGGCAGACC TGTCTGCATT GGTGAGGGAA 
GCCTCTGTCA ATGCTCTGAG AGCCTACCTC CTGACCCAAC CCAACCCATC TTACACAGGT 
CACAGCTACT CCAACGGCCC TGTAGCCGAC ATCCGGGTCA GCAAGCAGAA CTTTGAGGAC 
GCCTTCAAGA AAGTACGGCC GTCTGTGTCT AAAAAGGACC AGCGTATGTA CGAGCAGCTA 
AAGGAATCTC TTACAAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021466064.1
CDS	277..2817
Translation	MDYGRRNKTAFRIQVEKVHGVICDESGLNDLENKHLAKRARHSEKDAGDGSSNLDDTTDSDEDLPEQPPINLMNNSLMSLYKKGNPESGASPRRDKSASDSPAPEAHSTPQSVGTKVSAGGWFIDKGRGLEHDSILIDLCEEEAASPAGNKQTDASMLEPEKTPKKGTFKKSKRRKEGASKGNDGDIEASIMNKKARTKSIELQYSTLKFEDVGGNEDTLTEVCKLLIHMRHPEVYQQLGVVPPRGFLLHGPPGCGKTLLAQAVAGEMELPMLKVSAPELVSGVSGESEQKLRELFEQAVTSSPCILFIDEIDAITPKREVASKDMERRIVAQLLTCMDDLNSLAVTAQVMVIGATNRPDSLDPALRRAGRFDKEICLGIPDEAARLRILKTLCRKLKLPEDFDYRQLARLTPGYVGADLMALCREAAMSAVNRVLLERQDKNQGLVPMAEERAESEGLQGTGPLPQGPERFQASADTVIQEMTDRSHVPQGDKQQLPLATETTFRGELCRLLSLLKSSESLSEEQLAGLCILMSDFQGSLASVQPSAKREGFATVPDVTWEDVGALHDIREELTMAILAPVRSPEQFKALGLSAPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRGRNSAPCVIFFDEIDALCPRRSGHESGASVRVVNQLLTEMDGLETRRQVFIMAATNRPDIIDPAVLRPGRLDKTLYVGLPPPSDRHAILLTITKGGSRPHLEQDVSLEEMAHDVRSDCFTGADLSALVREASVNALRAYLLTQPNPSYTGHSYSNGPVADIRVSKQNFEDAFKKVRPSVSKKDQRMYEQLKESLTR

Target ORF information:

RefSeq Version	XM_021610389.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss nuclear VCP-like (nvl), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021610389.1

ORF Insert Sequence:

ATGGACTATG GCAGGCGAAA CAAGACTGCA TTCAGGATTC AGGTTGAAAA AGTCCATGGA 
GTGATATGCG ATGAATCTGG ACTGAATGAT CTGGAGAACA AGCACTTGGC CAAGAGAGCC 
AGACACAGTG AGAAGGATGC TGGGGATGGC AGCAGTAACT TGGATGACAC TACTGATAGT 
GACGAGGACC TTCCTGAACA ACCGCCCATC AACCTCATGA ATAACTCCCT GATGTCCCTG 
TACAAGAAGG GGAACCCAGA GAGTGGGGCA TCTCCCAGGA GGGACAAGTC TGCATCAGAC 
AGCCCTGCCC CTGAGGCTCA CTCCACCCCT CAGAGTGTTG GTACCAAGGT GTCTGCAGGG 
GGCTGGTTCA TAGACAAGGG CAGAGGCCTG GAGCATGACA GCATCCTCAT TGACCTGTGT 
GAAGAGGAGG CAGCCAGTCC TGCAGGAAAT AAGCAGACAG ATGCCTCAAT GCTTGAGCCT 
GAGAAGACAC CAAAAAAAGG CACATTTAAA AAATCTAAAC GGCGGAAAGA GGGAGCGTCA 
AAGGGCAATG ATGGTGACAT CGAGGCCAGT ATCATGAACA AGAAAGCGAG GACCAAATCC 
ATTGAACTAC AGTATTCAAC ATTAAAGTTT GAAGATGTTG GAGGCAATGA GGACACATTG 
ACGGAGGTGT GTAAGCTGCT GATACATATG CGTCACCCAG AGGTGTACCA GCAGTTGGGG 
GTAGTCCCTC CACGGGGGTT CCTGCTCCAT GGCCCCCCTG GCTGTGGAAA AACCCTGCTG 
GCCCAAGCTG TGGCTGGGGA AATGGAGCTG CCCATGCTGA AGGTATCGGC ACCAGAGCTG 
GTGTCTGGGG TGTCAGGGGA GTCTGAGCAG AAGCTGAGAG AGCTGTTTGA GCAGGCTGTG 
ACCAGCTCCC CTTGCATCCT GTTCATTGAT GAGATTGATG CCATCACTCC TAAGCGAGAG 
GTGGCCTCCA AGGACATGGA GAGACGGATT GTTGCTCAGC TGCTCACCTG CATGGATGAC 
CTGAACTCAT TAGCGGTCAC AGCCCAGGTG ATGGTTATTG GTGCCACCAA CCGGCCAGAC 
TCTCTGGACC CAGCGCTGCG CCGGGCTGGA CGCTTTGACA AAGAAATCTG TCTGGGGATA 
CCTGATGAAG CAGCTCGTCT CAGAATTCTG AAGACCCTGT GTCGGAAGCT GAAGCTCCCT 
GAGGACTTTG ACTACCGGCA GCTGGCGCGT CTTACCCCGG GGTACGTGGG AGCTGACCTC 
ATGGCGCTGT GCCGCGAGGC CGCCATGAGC GCCGTCAACC GGGTCCTGCT GGAGAGGCAG 
GACAAGAACC AGGGGCTGGT CCCCATGGCG GAGGAGAGAG CAGAGTCTGA GGGGCTTCAG 
GGCACAGGTC CTCTCCCACA GGGCCCAGAG AGATTCCAGG CCTCAGCAGA CACAGTGATC 
CAGGAGATGA CAGACCGTAG CCACGTACCA CAGGGAGACA AGCAGCAGCT GCCTCTGGCT 
ACTGAGACAA CCTTCAGGGG GGAGCTTTGT CGCCTGCTGT CCCTGCTGAA GAGCAGTGAG 
TCGCTGTCTG AGGAGCAGCT TGCTGGCTTG TGCATCCTCA TGTCAGACTT CCAGGGTTCC 
CTGGCCAGCG TACAACCCTC TGCAAAGCGT GAAGGCTTCG CCACGGTGCC AGACGTCACC 
TGGGAGGACG TGGGGGCCCT GCATGACATC CGCGAAGAGC TCACCATGGC CATCCTGGCC 
CCTGTGCGTT CTCCTGAGCA GTTCAAAGCT CTGGGTCTCA GTGCTCCTGC AGGGGTGTTG 
CTAGCTGGGC CTCCCGGTTG TGGAAAGACC CTGCTGGCCA AGGCTGTTGC CAATGAGTCA 
GGACTTAACT TCATCTCTGT CAAGGGGCCA GAGCTGCTGA ACATGTATGT GGGAGAGAGC 
GAGCGGGCCG TGCGACAGGT GTTCCAGAGA GGACGGAACT CTGCCCCTTG TGTCATCTTT 
TTCGACGAGA TCGATGCACT GTGCCCCCGC CGCTCCGGGC ATGAGTCTGG AGCCAGTGTC 
CGTGTGGTCA ACCAGCTGCT TACTGAGATG GATGGACTGG AGACCCGGCG GCAGGTCTTC 
ATCATGGCGG CTACCAATAG ACCAGATATC ATTGATCCTG CTGTGCTGAG ACCAGGTCGT 
CTGGACAAGA CCCTGTATGT GGGCCTCCCA CCTCCATCAG ACCGGCACGC CATCTTACTC 
ACCATCACCA AGGGGGGCAG CAGGCCTCAT CTGGAGCAGG ATGTGAGTCT GGAGGAGATG 
GCCCATGACG TGCGCAGCGA CTGCTTCACT GGGGCAGACC TGTCTGCATT GGTGAGGGAA 
GCCTCTGTCA ATGCTCTGAG AGCCTACCTC CTGACCCAAC CCAACCCATC TTACACAGGT 
CACAGCTACT CCAACGGCCC TGTAGCCGAC ATCCGGGTCA GCAAGCAGAA CTTTGAGGAC 
GCCTTCAAGA AAGTACGGCC GTCTGTGTCT AAAAAGGACC AGCGTATGTA CGAGCAGCTA 
AAGGAATCTC TTACAAGGTG A