ankle1 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	ankle1
Entrez Gene ID	110528311
Full Name	ankyrin repeat and LEM domain containing 1
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021610318.1	XP_021465993.1	ankyrin repeat and LEM domain-containing protein 1

Pathways from BioSystems
Gene Ontology

The following ankle1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ankle1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf51201	XM_021610318.1 Latest version!	Oncorhynchus mykiss ankyrin repeat and LEM domain containing 1 (ankle1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ankle1 ( XM_021610318.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021465993.1 Oncorhynchus mykiss ankyrin repeat and LEM domain containing 1 (ankle1), transcript variant X1, mRNA.

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CloneID	OOf51201
Clone ID Related Accession (Same CDS sequence)	XM_021610318.1
Accession Version	XM_021610318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	ankyrin repeat and LEM domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCATC GAAGCAAGAC CAGTCTGGCA ACACAGCTGT GCAAAGCTGT GAATGACGGA 
GAACCAAGAA CCGTGCAGAT TCTTCTCGCA CAAGGAGCAA ATCCCAATCT TGTCGGAAGT 
AAAGGTGTTG CTGCTGTGCA TTTGGCAGTT GGCAAAGAGA CTGAGAAAAG TATACGCTGC 
CTAAAGTTAA TTCTACAACA TGGGGCAGAC CCCAACATCC GGTCTTCGGA TGGCTTGACA 
CCCCTGCACG TAGCAGCGTT ATGGGGTTGC TATCAGAATC TCAAACTGCT GTTAAAGAAT 
GGAGGGAACG GCAACTTAAA GGATCAGGAA GGGAACAAAC CAGGTGATCT GGCACAACAA 
CAGGACAACC GCAGATGTGC CCACCTCCTT CAGGAGCACC AGTCTCAGTC CTTGGACACA 
GAGGAGGAAG ATCTGCCTCA ATTCCAATAC TCTGTTTACA ATGGCCAAGG TGACGCGTCA 
AGCTACGCTG AGTCCGAAGA CTGCAGTGTT CTCTCTCACA GCATGTCCTT GCTGAGTGAC 
TTCGGGGAGG GCCCACTGAG TAGCACACGT CTGACCTCGT TTTTTGAACT GTCTGCTATT 
AGCAACAGGC ACAGTTGGAG AAGAGGATCC CATTCAGGGG CGTCTGATTG TAAATCTAAC 
CCTGAGGTGC GCCACACCAA CGAGTGGGAC ACGGACTCTG ACTGGGAGCC TCCGTCAATG 
TTATCCAGCA CTCGTATGTC CGTTGCAGGT CCTAGAGCCG CACTTCCTGT ACTTCGGGAG 
GACGTACCAC TCGCTGACTG CAGTGCGCTG CATCCGTCTG TAACTGGGAA CAGACTCTCT 
CCTCCTCCGT TGGACCTCCT CCCTCCTTGC TTTTCACGAC GCACGAGCCG CAAGAGTGTG 
AGCTTCAGAG AGGAAGTGGA CGAATACTTC CCCGTTTTTA GTTCAGAATC TCCGAGGCGG 
GAGCCAGGGG GTCAGGGTAC TGCCTACTCT AAAGACTATA CTCTCGACTT CTCGGAATAC 
TCAGAGTTCC TGGATCCAGA GCGCATGGCC ACCGTCCTAC ATCACCAGGG GATCGACGTC 
ACGTCCCCTG AACATGTGTT TGTATTCTCC AAGGATAATA ATGAGTGCAC TGAGATCGAC 
ATGGAAAAAA CATTTGTTGG ACACTTCCCA TTTGAGGAAG AGGACGGAGA AGTCGTTGAG 
ACCCAGGTAG AAGAAGTGAA GGTCCCTGAA CGAGCATTAT GTGGCTCTGC TGGCAGCAGC 
AGCAGTAGCA GCGGCACTAG TCGGTACAGC AGCTGTGATA GTGACCACTA CATTACGACC 
CTGGAGGCGT CCCCCAGGCG GGTCTCGCCC TCTGAAGAGG AGCGTAAAGT GAAAAGCAGA 
GGTGTCGAAG ACGACAAACA CACACAAAAT CCTATGAACA CTAACAATGT AGATACTGAT 
GAAACTCCCA ACAGCTCAGA GAGGTCAAAA CAAGCCATGG TAGAACTACC AGCCATGGTA 
GATAACCTTA CATTGACTGA CAAAATATCT CCAACCAAAG GTCCAGGTTG TGAAAAAATA 
TTTCCAGATG CTGTCTTAGC GGGTAATGTT GAAGAGTTGT CAACAAGACC GACTGAATGC 
ACTCCCAGAC CTGGTTCTAC TCCAGCGTTG GAGGAGGACC TGCCTCTGAC CCCAAGTCCC 
TTTGTCACTG GTAGGACGCA CTCGCGGCTG AGTCGCTGCT CGGTCATTAG CGGCAAGACC 
CCCGAAAGCC TCCTCTCCAC CTCCTCGCTG TTTGAGCAGA CCTTGCCTAC GCCGACACGC 
ACACGCCGCC AAAACATCAA GTCGCAAGGC AGCGACAACG TCTTTTACGA CACGCCATGC 
GGGCGTAGTC GCACCCCAGT CTTTTCTGGA AACGGGACAG GTAGTACTTC TGGGGACTCA 
TTTGTCATCT ACGAGAGCCA AGAGCCTCAA TCTAGCACTC TCAGAGCCGA TAGAGGTCAA 
GGGTCATATG TCAGTGCCAG CCAAGCGGAC ACCCTCATCA TCGGCAAGGA CGACAGAGGT 
CAGGGATCTT GTGTTAGTGC CAGCCAAGCG GACACCCTCA TCATCGCCAA GGACGACAGA 
GGTCAGGGAT CTTGTGTTAG TGCCAGCCAA GCGGACACCC TCATCTCTAA AACCATGGCT 
GACACAGTCA TCGTAACTCC AAGTTTAGCT GACAAATTCT CTTTACTGGA TAATTACTTT 
ACTTTAAAAA ATCCTCACAA AGAGACTTTA CCCACTTATC TGAGAAAAGA CGTTCTTGAA 
AGTGGTGAAT TTCTAACCGA CGATTTGTCC AGCTCCAGTG ATGCAGTGAC CAAGGAGGGG 
ACAGTTGGTA GCTTTGGACC AAGAGTGGAG GAATCCTGGA CCACAGAGGA GGCCGACTCA 
AGCTCATCTT TGTCAAGTTC CCAGACTCCA TCTTCATCCA GCTCCAGTTA CTTCTCTCCA 
AAAAGAGATC AGCCCCCCTC GACCCCAGGC ACTACCCCGC GCTACAGCAT GAGTCGACTC 
TCCCGCGTCC ACAAGCCCCA GCGGCTGGCC AGCCTCTCCT ACACCCCAGG CGGACGTCCT 
GTGATACCAG ACCAGGAGGA ACCGGTGGAG TACCTCTACA CGGACACGGA GCAGGGTCAC 
GAACTGATCG AGACCCACGT CCCACCTGCG GCCAACACCT CGCTCAGCTC CAGCATGAGC 
ACGTCCAGCG GCGAGGACAC AGTGCTGTAC GACTGGCGCT CCCTGCAGGG TGGTGGAGGA 
ACGGAGGCCA AAGGGAAGGA GAACCAGGAG CCCGTGGTGG CTCGGGAGTG GTCAGAGACC 
AAATGCCTGA CGGACAAGGA GCTGAGACGC AGGCTTGTGG AGATGGGAGA GAGTCCGGGA 
CCCATAAGTC ACCGCACCCG GCCAGTGTAC ATGAAGAGGC TAAGGCGGCT GTTGCTGGAG 
CCGGACACTC AACAACAACT GCCGGACCAA CCCCGAGGTC CTGCGCAGGG ACACAGTCCA 
GAGCTGTGTG TGGCCCTGCG GACGTTGGTG CTGCCCGACT GCCAGGACGA CGAGCTGGCG 
CTGTGCCAGC AGTTTGACCA ACCAGACCAG AACAGGAAGT GGCGGGAGGG CGTCATCAAG 
TCCAGCTTCA ACTACCTGCT ACTGGACCCC AGAGTAACAA AGAACCTCCC GTATCGGAGT 
CATTCCATGA GCCCTATAGA TTGTTTTCAG ACTTTCATCA GCGCTATATT CTACGTGGGC 
AAAGGCAAGC GCTCCCGACC CTACAGCCAT CTGTACGAGG CTCTAGACTA CCACCGAGGA 
GATAAGACTT CTAAGAAACT GTGCTCCAAG GTGCAGCACA TCCTCCAGGT TTGGAAGGCC 
GAGCAGGGGG TGATCTCTCT GCACTGCTTC CAGAACGTCA TCCCCGTGGA GGCCTACACG 
CGTGAGGCCG TCATGGTGGA CGCCATTGGG TTGAAGATGC TGACCAACCA GAAGCGAGGG 
GACTACTATG GGGTGGTGTC CACGTGGCAG TTGAAGAGGA AGAGGGAGCT GGGGGTGCAC 
CTGCTCTACA GGGCCATGCA GATCTTCCTG GCTGAGGGAG AGAGGCAGCT CAGACCTCCT 
GATATCAGGG TGGGACAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021465993.1
CDS	289..3909
Translation	MQHRSKTSLATQLCKAVNDGEPRTVQILLAQGANPNLVGSKGVAAVHLAVGKETEKSIRCLKLILQHGADPNIRSSDGLTPLHVAALWGCYQNLKLLLKNGGNGNLKDQEGNKPGDLAQQQDNRRCAHLLQEHQSQSLDTEEEDLPQFQYSVYNGQGDASSYAESEDCSVLSHSMSLLSDFGEGPLSSTRLTSFFELSAISNRHSWRRGSHSGASDCKSNPEVRHTNEWDTDSDWEPPSMLSSTRMSVAGPRAALPVLREDVPLADCSALHPSVTGNRLSPPPLDLLPPCFSRRTSRKSVSFREEVDEYFPVFSSESPRREPGGQGTAYSKDYTLDFSEYSEFLDPERMATVLHHQGIDVTSPEHVFVFSKDNNECTEIDMEKTFVGHFPFEEEDGEVVETQVEEVKVPERALCGSAGSSSSSSGTSRYSSCDSDHYITTLEASPRRVSPSEEERKVKSRGVEDDKHTQNPMNTNNVDTDETPNSSERSKQAMVELPAMVDNLTLTDKISPTKGPGCEKIFPDAVLAGNVEELSTRPTECTPRPGSTPALEEDLPLTPSPFVTGRTHSRLSRCSVISGKTPESLLSTSSLFEQTLPTPTRTRRQNIKSQGSDNVFYDTPCGRSRTPVFSGNGTGSTSGDSFVIYESQEPQSSTLRADRGQGSYVSASQADTLIIGKDDRGQGSCVSASQADTLIIAKDDRGQGSCVSASQADTLISKTMADTVIVTPSLADKFSLLDNYFTLKNPHKETLPTYLRKDVLESGEFLTDDLSSSSDAVTKEGTVGSFGPRVEESWTTEEADSSSSLSSSQTPSSSSSSYFSPKRDQPPSTPGTTPRYSMSRLSRVHKPQRLASLSYTPGGRPVIPDQEEPVEYLYTDTEQGHELIETHVPPAANTSLSSSMSTSSGEDTVLYDWRSLQGGGGTEAKGKENQEPVVAREWSETKCLTDKELRRRLVEMGESPGPISHRTRPVYMKRLRRLLLEPDTQQQLPDQPRGPAQGHSPELCVALRTLVLPDCQDDELALCQQFDQPDQNRKWREGVIKSSFNYLLLDPRVTKNLPYRSHSMSPIDCFQTFISAIFYVGKGKRSRPYSHLYEALDYHRGDKTSKKLCSKVQHILQVWKAEQGVISLHCFQNVIPVEAYTREAVMVDAIGLKMLTNQKRGDYYGVVSTWQLKRKRELGVHLLYRAMQIFLAEGERQLRPPDIRVGQ

Target ORF information:

RefSeq Version	XM_021610318.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss ankyrin repeat and LEM domain containing 1 (ankle1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021610318.1

ORF Insert Sequence:

ATGCAGCATC GAAGCAAGAC CAGTCTGGCA ACACAGCTGT GCAAAGCTGT GAATGACGGA 
GAACCAAGAA CCGTGCAGAT TCTTCTCGCA CAAGGAGCAA ATCCCAATCT TGTCGGAAGT 
AAAGGTGTTG CTGCTGTGCA TTTGGCAGTT GGCAAAGAGA CTGAGAAAAG TATACGCTGC 
CTAAAGTTAA TTCTACAACA TGGGGCAGAC CCCAACATCC GGTCTTCGGA TGGCTTGACA 
CCCCTGCACG TAGCAGCGTT ATGGGGTTGC TATCAGAATC TCAAACTGCT GTTAAAGAAT 
GGAGGGAACG GCAACTTAAA GGATCAGGAA GGGAACAAAC CAGGTGATCT GGCACAACAA 
CAGGACAACC GCAGATGTGC CCACCTCCTT CAGGAGCACC AGTCTCAGTC CTTGGACACA 
GAGGAGGAAG ATCTGCCTCA ATTCCAATAC TCTGTTTACA ATGGCCAAGG TGACGCGTCA 
AGCTACGCTG AGTCCGAAGA CTGCAGTGTT CTCTCTCACA GCATGTCCTT GCTGAGTGAC 
TTCGGGGAGG GCCCACTGAG TAGCACACGT CTGACCTCGT TTTTTGAACT GTCTGCTATT 
AGCAACAGGC ACAGTTGGAG AAGAGGATCC CATTCAGGGG CGTCTGATTG TAAATCTAAC 
CCTGAGGTGC GCCACACCAA CGAGTGGGAC ACGGACTCTG ACTGGGAGCC TCCGTCAATG 
TTATCCAGCA CTCGTATGTC CGTTGCAGGT CCTAGAGCCG CACTTCCTGT ACTTCGGGAG 
GACGTACCAC TCGCTGACTG CAGTGCGCTG CATCCGTCTG TAACTGGGAA CAGACTCTCT 
CCTCCTCCGT TGGACCTCCT CCCTCCTTGC TTTTCACGAC GCACGAGCCG CAAGAGTGTG 
AGCTTCAGAG AGGAAGTGGA CGAATACTTC CCCGTTTTTA GTTCAGAATC TCCGAGGCGG 
GAGCCAGGGG GTCAGGGTAC TGCCTACTCT AAAGACTATA CTCTCGACTT CTCGGAATAC 
TCAGAGTTCC TGGATCCAGA GCGCATGGCC ACCGTCCTAC ATCACCAGGG GATCGACGTC 
ACGTCCCCTG AACATGTGTT TGTATTCTCC AAGGATAATA ATGAGTGCAC TGAGATCGAC 
ATGGAAAAAA CATTTGTTGG ACACTTCCCA TTTGAGGAAG AGGACGGAGA AGTCGTTGAG 
ACCCAGGTAG AAGAAGTGAA GGTCCCTGAA CGAGCATTAT GTGGCTCTGC TGGCAGCAGC 
AGCAGTAGCA GCGGCACTAG TCGGTACAGC AGCTGTGATA GTGACCACTA CATTACGACC 
CTGGAGGCGT CCCCCAGGCG GGTCTCGCCC TCTGAAGAGG AGCGTAAAGT GAAAAGCAGA 
GGTGTCGAAG ACGACAAACA CACACAAAAT CCTATGAACA CTAACAATGT AGATACTGAT 
GAAACTCCCA ACAGCTCAGA GAGGTCAAAA CAAGCCATGG TAGAACTACC AGCCATGGTA 
GATAACCTTA CATTGACTGA CAAAATATCT CCAACCAAAG GTCCAGGTTG TGAAAAAATA 
TTTCCAGATG CTGTCTTAGC GGGTAATGTT GAAGAGTTGT CAACAAGACC GACTGAATGC 
ACTCCCAGAC CTGGTTCTAC TCCAGCGTTG GAGGAGGACC TGCCTCTGAC CCCAAGTCCC 
TTTGTCACTG GTAGGACGCA CTCGCGGCTG AGTCGCTGCT CGGTCATTAG CGGCAAGACC 
CCCGAAAGCC TCCTCTCCAC CTCCTCGCTG TTTGAGCAGA CCTTGCCTAC GCCGACACGC 
ACACGCCGCC AAAACATCAA GTCGCAAGGC AGCGACAACG TCTTTTACGA CACGCCATGC 
GGGCGTAGTC GCACCCCAGT CTTTTCTGGA AACGGGACAG GTAGTACTTC TGGGGACTCA 
TTTGTCATCT ACGAGAGCCA AGAGCCTCAA TCTAGCACTC TCAGAGCCGA TAGAGGTCAA 
GGGTCATATG TCAGTGCCAG CCAAGCGGAC ACCCTCATCA TCGGCAAGGA CGACAGAGGT 
CAGGGATCTT GTGTTAGTGC CAGCCAAGCG GACACCCTCA TCATCGCCAA GGACGACAGA 
GGTCAGGGAT CTTGTGTTAG TGCCAGCCAA GCGGACACCC TCATCTCTAA AACCATGGCT 
GACACAGTCA TCGTAACTCC AAGTTTAGCT GACAAATTCT CTTTACTGGA TAATTACTTT 
ACTTTAAAAA ATCCTCACAA AGAGACTTTA CCCACTTATC TGAGAAAAGA CGTTCTTGAA 
AGTGGTGAAT TTCTAACCGA CGATTTGTCC AGCTCCAGTG ATGCAGTGAC CAAGGAGGGG 
ACAGTTGGTA GCTTTGGACC AAGAGTGGAG GAATCCTGGA CCACAGAGGA GGCCGACTCA 
AGCTCATCTT TGTCAAGTTC CCAGACTCCA TCTTCATCCA GCTCCAGTTA CTTCTCTCCA 
AAAAGAGATC AGCCCCCCTC GACCCCAGGC ACTACCCCGC GCTACAGCAT GAGTCGACTC 
TCCCGCGTCC ACAAGCCCCA GCGGCTGGCC AGCCTCTCCT ACACCCCAGG CGGACGTCCT 
GTGATACCAG ACCAGGAGGA ACCGGTGGAG TACCTCTACA CGGACACGGA GCAGGGTCAC 
GAACTGATCG AGACCCACGT CCCACCTGCG GCCAACACCT CGCTCAGCTC CAGCATGAGC 
ACGTCCAGCG GCGAGGACAC AGTGCTGTAC GACTGGCGCT CCCTGCAGGG TGGTGGAGGA 
ACGGAGGCCA AAGGGAAGGA GAACCAGGAG CCCGTGGTGG CTCGGGAGTG GTCAGAGACC 
AAATGCCTGA CGGACAAGGA GCTGAGACGC AGGCTTGTGG AGATGGGAGA GAGTCCGGGA 
CCCATAAGTC ACCGCACCCG GCCAGTGTAC ATGAAGAGGC TAAGGCGGCT GTTGCTGGAG 
CCGGACACTC AACAACAACT GCCGGACCAA CCCCGAGGTC CTGCGCAGGG ACACAGTCCA 
GAGCTGTGTG TGGCCCTGCG GACGTTGGTG CTGCCCGACT GCCAGGACGA CGAGCTGGCG 
CTGTGCCAGC AGTTTGACCA ACCAGACCAG AACAGGAAGT GGCGGGAGGG CGTCATCAAG 
TCCAGCTTCA ACTACCTGCT ACTGGACCCC AGAGTAACAA AGAACCTCCC GTATCGGAGT 
CATTCCATGA GCCCTATAGA TTGTTTTCAG ACTTTCATCA GCGCTATATT CTACGTGGGC 
AAAGGCAAGC GCTCCCGACC CTACAGCCAT CTGTACGAGG CTCTAGACTA CCACCGAGGA 
GATAAGACTT CTAAGAAACT GTGCTCCAAG GTGCAGCACA TCCTCCAGGT TTGGAAGGCC 
GAGCAGGGGG TGATCTCTCT GCACTGCTTC CAGAACGTCA TCCCCGTGGA GGCCTACACG 
CGTGAGGCCG TCATGGTGGA CGCCATTGGG TTGAAGATGC TGACCAACCA GAAGCGAGGG 
GACTACTATG GGGTGGTGTC CACGTGGCAG TTGAAGAGGA AGAGGGAGCT GGGGGTGCAC 
CTGCTCTACA GGGCCATGCA GATCTTCCTG GCTGAGGGAG AGAGGCAGCT CAGACCTCCT 
GATATCAGGG TGGGACAGTA G