LOC100135976 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100135976
Entrez Gene ID	100135976
Full Name	cGMP-gated channel
Synonyms	CNGA3
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001124315.1	NP_001117787.1	cGMP-gated channel

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Cyclic nucleotide-gated channel ?-3 (CNGA3) interacts with stereocilia tip-link cadherin 23 + exon 68 or alternatively with myosin VIIa, two proteins required for hair cell mechanotransduction.
Selvakumar D, Drescher MJ, Drescher DG
The Journal of biological chemistry288(10)7215-29(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC100135976 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100135976 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf01084	NM_001124315.1 Latest version!	Oncorhynchus mykiss cGMP-gated channel (LOC100135976), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100135976 ( NM_001124315.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001117787.1 Oncorhynchus mykiss cGMP-gated channel (LOC100135976), mRNA.

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CloneID	OOf01084
Clone ID Related Accession (Same CDS sequence)	NM_001124315.1
Accession Version	NM_001124315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-09
Organism	Oncorhynchus mykiss(rainbow trout)
Product	cGMP-gated channel
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF393839.1. ##Evidence-Data-START## Transcript exon combination :: AF393839.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN07203794 [ECO:0000350] ##Evidence-Data-END##

ATGGCAAAGA TCTGCACAGA CCAGTCCTAC CCGGCAACGA CCCGGCAACG GCCATCTCTC 
TGCACGCCAA ATGACAATCT GGACTCCATT GAGAACGGAA CCCCAAGGTC CAACTCAGAG 
TGTGAGGACA CCTCGTCAGA GATTCAGGGT GCTGTCTCTG TGTTAACCAG CAGACACCCA 
GAGCCTCACA GAGATTCCTT CACAGGGTCA GGAGCCCTGG CCAGGATGCA CCGCGAGGTG 
GAGAGACCGG ACTCCTTCCT GGAGCGCTTC AGGACCCCTG AGCTCAAAGA GGCCTCCAGC 
AGAGAGAGCA ACACACGGTC ACAGGGATAC CCCGACAGAG TCCGTAAGAG GAACCAAGTC 
GGTCAATGGC CGCTGGCCAT GTACAATATG AACAACTGCA ACAACACAGA TGACAAAAAA 
GATGACAAGA AGGACGAAAT AAAGAAAGAG GAGAAAAAAC AGGAAGCGCA AGGAGATGAG 
AAGAAAGAGG AGGAGAAAGA GGAGAAGAAA GAGGAGGAGA AAGAGGAGAA GAAAGAGGAG 
GAGCCTAAGA AGGAGGAGCC TAAGAAGGAG GAGCCTAAGA AGGAGGAGCC TAAGAAGGAG 
GAGAAGAAGA AGGAGGAGCC CCCGAAAGAG ATTTGGATCA TGGACCCATC AGCAGACAAA 
TACTACACAT GGCTCACCAT CATAGCAGCA CCATGTTTCT ACAACCTGAT GATGCTAGTG 
ACCAGGGCAT GTTTTAACGA ACTCCAGAAC ACATACACAA TGCTGTGGAT TGTCCTGGAC 
TACACCTCAG ACTTAGTCTA CTACGCAGAC ACATTCGTCA GGTCTAGAAC AGGTTATTTG 
GAACAGGGGC TGCTTGTGAA AGACAAACAA AAGCTTCTAG ACAAATACAG ACAAACGGCA 
CAGTTCAAAT GGGACGTCAT TTCAATGATA CCCACAGACC TGCTTTTTCT GAAAGTGGGC 
ATCAACAACC CCGAGCTCAG ACTGAACCGA CTCACAAAGA TGAACCGCCT CTTTGAGTAC 
TTTGACCGCA CTGAAACCAG AACCAGCTTC CCAACGTTTT CCGAATCAGC AACCTGGTAC 
TTTTACATCC TGATCATCAT CCACTGGAAT GCTTGCATCT TCTTCGCCAT CTTCAAGATC 
ATCGGGTTCG GGTCCGACAC CTGGGTGTAC CCAACATCAG TCACCCTGAG CACGGCCGCC 
TTTCCAGGAA GTATCATCTA CTGTCTCTAT TGGTCGACCC TGACCCTGAC CACCATCGGA 
GAAACCCCGC CCCCCGTCAG GGACTTTGAG TACCTCTTCG TGGTCGCCGA CTTCCTCATC 
GGCGTGCTGA TTTTCGCCAC CATTGTCGGT AATGTTGGCG CCATGATCTC CAACATGAAC 
AAAAACCGTG CGGACTTCCA GGCTAAGATC GACTCCATCA AGCAATTCAT GCAGTTCCGT 
AAGGTCTCCA AGGATCTGGA GGCCAGGGTC ATCAAGTGGT TTGACTACCT CTGGACGGAG 
AAGAAGACCT GCGATGAGAA GGAGGTGCTG AAGAACCTGC CGGACAAGCT GAAGGCCGAG 
ATTGCCATCG ACGTCCATCT GGATACCCTG AAGAAAGTGA AAATCTTCCA GGAATGCGAG 
GCTGGGCTGC TTATTGAGTT GGTGATGAAG CTGCAGCCTC AGGTGTTCTC CCCAGGAGAT 
TACATCTGTA AGAAGGGAGA CATCGGCAGA GAGATGTACA TCATTAAGGA AGGGAAGCTG 
GCTGTGGTTG CGGAAGACGG GGTGACACAG TGGTGTGTGC TCAGCGACGG AGCGTACTTC 
GGAGAGATCA GTATCCTAGG CATCAAGGGT TCCAAAGCTG GTAACAGGAG AACGGCCAAC 
ATCAGGAGCG TGGGTTACTC TGACCTCTTC GCTCTGTCGA AAGATGATCT CATGGAAGCT 
CTCACCGAGT ATCCCGAAGC CAAGAAGATC CTGGAAGAGA AGGGAAAGGC TACGCTGATG 
AAGGACAACA TGATCGATGA GGAAATCGCT AATGCAGGTG CCGACCCCAA GGACATGGAG 
GAGAAGATCC TCAGGCTGGA GAGTAATATG GAAATCATGA CGTCCAAGTT GGCCAAGATC 
ATGGCTGAGT TCACATCCGG CCAGGGCAAG ATTAAACAGA GGATCACCGA CATGGAGTCA 
ACAGTGAAGT CGGTCCGGCC CGAGGATATA TCTGAGGTGG TGGCCGACAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001117787.1
CDS	288..2501
Translation	MAKICTDQSYPATTRQRPSLCTPNDNLDSIENGTPRSNSECEDTSSEIQGAVSVLTSRHPEPHRDSFTGSGALARMHREVERPDSFLERFRTPELKEASSRESNTRSQGYPDRVRKRNQVGQWPLAMYNMNNCNNTDDKKDDKKDEIKKEEKKQEAQGDEKKEEEKEEKKEEEKEEKKEEEPKKEEPKKEEPKKEEPKKEEKKKEEPPKEIWIMDPSADKYYTWLTIIAAPCFYNLMMLVTRACFNELQNTYTMLWIVLDYTSDLVYYADTFVRSRTGYLEQGLLVKDKQKLLDKYRQTAQFKWDVISMIPTDLLFLKVGINNPELRLNRLTKMNRLFEYFDRTETRTSFPTFSESATWYFYILIIIHWNACIFFAIFKIIGFGSDTWVYPTSVTLSTAAFPGSIIYCLYWSTLTLTTIGETPPPVRDFEYLFVVADFLIGVLIFATIVGNVGAMISNMNKNRADFQAKIDSIKQFMQFRKVSKDLEARVIKWFDYLWTEKKTCDEKEVLKNLPDKLKAEIAIDVHLDTLKKVKIFQECEAGLLIELVMKLQPQVFSPGDYICKKGDIGREMYIIKEGKLAVVAEDGVTQWCVLSDGAYFGEISILGIKGSKAGNRRTANIRSVGYSDLFALSKDDLMEALTEYPEAKKILEEKGKATLMKDNMIDEEIANAGADPKDMEEKILRLESNMEIMTSKLAKIMAEFTSGQGKIKQRITDMESTVKSVRPEDISEVVADK

Target ORF information:

RefSeq Version	NM_001124315.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss cGMP-gated channel (LOC100135976), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001124315.1

ORF Insert Sequence:

ATGGCAAAGA TCTGCACAGA CCAGTCCTAC CCGGCAACGA CCCGGCAACG GCCATCTCTC 
TGCACGCCAA ATGACAATCT GGACTCCATT GAGAACGGAA CCCCAAGGTC CAACTCAGAG 
TGTGAGGACA CCTCGTCAGA GATTCAGGGT GCTGTCTCTG TGTTAACCAG CAGACACCCA 
GAGCCTCACA GAGATTCCTT CACAGGGTCA GGAGCCCTGG CCAGGATGCA CCGCGAGGTG 
GAGAGACCGG ACTCCTTCCT GGAGCGCTTC AGGACCCCTG AGCTCAAAGA GGCCTCCAGC 
AGAGAGAGCA ACACACGGTC ACAGGGATAC CCCGACAGAG TCCGTAAGAG GAACCAAGTC 
GGTCAATGGC CGCTGGCCAT GTACAATATG AACAACTGCA ACAACACAGA TGACAAAAAA 
GATGACAAGA AGGACGAAAT AAAGAAAGAG GAGAAAAAAC AGGAAGCGCA AGGAGATGAG 
AAGAAAGAGG AGGAGAAAGA GGAGAAGAAA GAGGAGGAGA AAGAGGAGAA GAAAGAGGAG 
GAGCCTAAGA AGGAGGAGCC TAAGAAGGAG GAGCCTAAGA AGGAGGAGCC TAAGAAGGAG 
GAGAAGAAGA AGGAGGAGCC CCCGAAAGAG ATTTGGATCA TGGACCCATC AGCAGACAAA 
TACTACACAT GGCTCACCAT CATAGCAGCA CCATGTTTCT ACAACCTGAT GATGCTAGTG 
ACCAGGGCAT GTTTTAACGA ACTCCAGAAC ACATACACAA TGCTGTGGAT TGTCCTGGAC 
TACACCTCAG ACTTAGTCTA CTACGCAGAC ACATTCGTCA GGTCTAGAAC AGGTTATTTG 
GAACAGGGGC TGCTTGTGAA AGACAAACAA AAGCTTCTAG ACAAATACAG ACAAACGGCA 
CAGTTCAAAT GGGACGTCAT TTCAATGATA CCCACAGACC TGCTTTTTCT GAAAGTGGGC 
ATCAACAACC CCGAGCTCAG ACTGAACCGA CTCACAAAGA TGAACCGCCT CTTTGAGTAC 
TTTGACCGCA CTGAAACCAG AACCAGCTTC CCAACGTTTT CCGAATCAGC AACCTGGTAC 
TTTTACATCC TGATCATCAT CCACTGGAAT GCTTGCATCT TCTTCGCCAT CTTCAAGATC 
ATCGGGTTCG GGTCCGACAC CTGGGTGTAC CCAACATCAG TCACCCTGAG CACGGCCGCC 
TTTCCAGGAA GTATCATCTA CTGTCTCTAT TGGTCGACCC TGACCCTGAC CACCATCGGA 
GAAACCCCGC CCCCCGTCAG GGACTTTGAG TACCTCTTCG TGGTCGCCGA CTTCCTCATC 
GGCGTGCTGA TTTTCGCCAC CATTGTCGGT AATGTTGGCG CCATGATCTC CAACATGAAC 
AAAAACCGTG CGGACTTCCA GGCTAAGATC GACTCCATCA AGCAATTCAT GCAGTTCCGT 
AAGGTCTCCA AGGATCTGGA GGCCAGGGTC ATCAAGTGGT TTGACTACCT CTGGACGGAG 
AAGAAGACCT GCGATGAGAA GGAGGTGCTG AAGAACCTGC CGGACAAGCT GAAGGCCGAG 
ATTGCCATCG ACGTCCATCT GGATACCCTG AAGAAAGTGA AAATCTTCCA GGAATGCGAG 
GCTGGGCTGC TTATTGAGTT GGTGATGAAG CTGCAGCCTC AGGTGTTCTC CCCAGGAGAT 
TACATCTGTA AGAAGGGAGA CATCGGCAGA GAGATGTACA TCATTAAGGA AGGGAAGCTG 
GCTGTGGTTG CGGAAGACGG GGTGACACAG TGGTGTGTGC TCAGCGACGG AGCGTACTTC 
GGAGAGATCA GTATCCTAGG CATCAAGGGT TCCAAAGCTG GTAACAGGAG AACGGCCAAC 
ATCAGGAGCG TGGGTTACTC TGACCTCTTC GCTCTGTCGA AAGATGATCT CATGGAAGCT 
CTCACCGAGT ATCCCGAAGC CAAGAAGATC CTGGAAGAGA AGGGAAAGGC TACGCTGATG 
AAGGACAACA TGATCGATGA GGAAATCGCT AATGCAGGTG CCGACCCCAA GGACATGGAG 
GAGAAGATCC TCAGGCTGGA GAGTAATATG GAAATCATGA CGTCCAAGTT GGCCAAGATC 
ATGGCTGAGT TCACATCCGG CCAGGGCAAG ATTAAACAGA GGATCACCGA CATGGAGTCA 
ACAGTGAAGT CGGTCCGGCC CGAGGATATA TCTGAGGTGG TGGCCGACAA GTAG