mkrn1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	mkrn1
Entrez Gene ID	777952
Full Name	makorin ring finger protein 1
Synonyms	Makorin-1
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001078582.1	NP_001072050.1	probable E3 ubiquitin-protein ligase makorin-1

Pathways from BioSystems
Gene Ontology

The following mkrn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mkrn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo00031	NM_001078582.1 Latest version!	Takifugu rubripes makorin ring finger protein 1 (mkrn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mkrn1 ( NM_001078582.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> NP_001072050.1 Takifugu rubripes makorin ring finger protein 1 (mkrn1), mRNA.

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CloneID	OTo00031
Clone ID Related Accession (Same CDS sequence)	NM_001078582.1
Accession Version	NM_001078582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Takifugu rubripes(torafugu)
Product	probable E3 ubiquitin-protein ligase makorin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB182269.1. ##Evidence-Data-START## Transcript exon combination :: AB182269.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02369525, SAMN02369526 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGG CAGCAGTCGC ATCTACGGTG ACTTTACCAG TGACCGGGGG GTGGACCAAA 
CACGTAACTT GCAGGTATTT CATGCACGGT CTGTGCAAAG AAGGGGACAA CTGTCGCTAT 
TCGCATGATC TGACCAGCAG CAAACCCGCA GCCATGATGT GCAAGTTCTT CCAGAAGGGA 
AACTGTGTGT TTGGGGAGCG ATGCAGGTTT GAACATTGTA AACCAACAAA GAGCGAGGAG 
GTCTCGAACC CCCAGATGTT GCTATTAAGC TCCACCCCAC CGCCGATTGA TCCAGAATGC 
AGTGAGTCAG GCCCTCGCCT GAAGACTCAG GACTGGGCCA ATGCTGCCGA GTTTGTTCCA 
GGACAGCCTT ACTGTGGCCG GGCTGAGTCG GTGGATGTGG AGATCTCCAT CCCTCTCATT 
GAAGAGCTCA ACGGTGATGC CACCACTGAT AAGGAAGAGC TTCGAAAGCA GCTCTGCCCA 
TATGCTGCTG TAGGAGAGTG CCGCTACGGC GTCAACTGTG CGTACCTGCA CGGGGATGTG 
TGTGACATGT GTGGCCTGCA GGTGCTCCAC CCCACCGATA GCAGCCAGCG CTCCGAGCAC 
ACCAAGGCCT GCATCGAGGC CCATGAAAAG GACATGGAGA TCTCGTTTGC CATCCAACGC 
AGCAAAGACA TGATGTGCGG CGTGTGCATG GAGGTGGTGT TTGAGAAGGC CAATCCCAGC 
GAGCGACGCT TCGGAATCCT CTCCAACTGC AGCCACTGCT ACTGTCTGAA GTGCATCCGC 
AAGTGGAGGA GTGCCAAGCA GTTCGAAAGC AAGATTATCA AGTCTTGTCC AGAGTGTCGA 
ATCACCTCCA ACTTTGTCAT CCCAAGTGAA TATTGGGTGG AAGATAAAGA AGACAAACAG 
AAGCTCATCC AGAAATACAA GGATGGAATG GGGAGGAAAC CCTGTCGATA CTTTGACGAG 
GGTCGTGGAA TTTGCCCTTT CGGTGCCAAT TGCTTCTACA AACACGCCTT TCCTGATGGT 
CGTCTGGAGG AGGCTCAGCC ACAGCGAAGA CAGACTGGAT CCAGCAGCAG GAACCGGAAC 
TCCAGGAGGA CACAACTGTG GGACATCATT GACGAGCGGG AAAGCACCGG TTCCCTGGAC 
AATGACGATG AGGAGATGGT GACATTTGAG CTGAGCGAGA TGCTCCTCAT GCTGCTCGCT 
GCAGGAAATG ATGAAGAGGT GACAGATTCA GAGGATGAGT GGGACTTGTT TCACGAGGAG 
CTGGACGATT TCTATGAGAT TTACCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072050.1
CDS	51..1340
Translation	MAEAAVASTVTLPVTGGWTKHVTCRYFMHGLCKEGDNCRYSHDLTSSKPAAMMCKFFQKGNCVFGERCRFEHCKPTKSEEVSNPQMLLLSSTPPPIDPECSESGPRLKTQDWANAAEFVPGQPYCGRAESVDVEISIPLIEELNGDATTDKEELRKQLCPYAAVGECRYGVNCAYLHGDVCDMCGLQVLHPTDSSQRSEHTKACIEAHEKDMEISFAIQRSKDMMCGVCMEVVFEKANPSERRFGILSNCSHCYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYWVEDKEDKQKLIQKYKDGMGRKPCRYFDEGRGICPFGANCFYKHAFPDGRLEEAQPQRRQTGSSSRNRNSRRTQLWDIIDERESTGSLDNDDEEMVTFELSEMLLMLLAAGNDEEVTDSEDEWDLFHEELDDFYEIYL

Target ORF information:

RefSeq Version	NM_001078582.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes makorin ring finger protein 1 (mkrn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001078582.1

ORF Insert Sequence:

ATGGCGGAGG CAGCAGTCGC ATCTACGGTG ACTTTACCAG TGACCGGGGG GTGGACCAAA 
CACGTAACTT GCAGGTATTT CATGCACGGT CTGTGCAAAG AAGGGGACAA CTGTCGCTAT 
TCGCATGATC TGACCAGCAG CAAACCCGCA GCCATGATGT GCAAGTTCTT CCAGAAGGGA 
AACTGTGTGT TTGGGGAGCG ATGCAGGTTT GAACATTGTA AACCAACAAA GAGCGAGGAG 
GTCTCGAACC CCCAGATGTT GCTATTAAGC TCCACCCCAC CGCCGATTGA TCCAGAATGC 
AGTGAGTCAG GCCCTCGCCT GAAGACTCAG GACTGGGCCA ATGCTGCCGA GTTTGTTCCA 
GGACAGCCTT ACTGTGGCCG GGCTGAGTCG GTGGATGTGG AGATCTCCAT CCCTCTCATT 
GAAGAGCTCA ACGGTGATGC CACCACTGAT AAGGAAGAGC TTCGAAAGCA GCTCTGCCCA 
TATGCTGCTG TAGGAGAGTG CCGCTACGGC GTCAACTGTG CGTACCTGCA CGGGGATGTG 
TGTGACATGT GTGGCCTGCA GGTGCTCCAC CCCACCGATA GCAGCCAGCG CTCCGAGCAC 
ACCAAGGCCT GCATCGAGGC CCATGAAAAG GACATGGAGA TCTCGTTTGC CATCCAACGC 
AGCAAAGACA TGATGTGCGG CGTGTGCATG GAGGTGGTGT TTGAGAAGGC CAATCCCAGC 
GAGCGACGCT TCGGAATCCT CTCCAACTGC AGCCACTGCT ACTGTCTGAA GTGCATCCGC 
AAGTGGAGGA GTGCCAAGCA GTTCGAAAGC AAGATTATCA AGTCTTGTCC AGAGTGTCGA 
ATCACCTCCA ACTTTGTCAT CCCAAGTGAA TATTGGGTGG AAGATAAAGA AGACAAACAG 
AAGCTCATCC AGAAATACAA GGATGGAATG GGGAGGAAAC CCTGTCGATA CTTTGACGAG 
GGTCGTGGAA TTTGCCCTTT CGGTGCCAAT TGCTTCTACA AACACGCCTT TCCTGATGGT 
CGTCTGGAGG AGGCTCAGCC ACAGCGAAGA CAGACTGGAT CCAGCAGCAG GAACCGGAAC 
TCCAGGAGGA CACAACTGTG GGACATCATT GACGAGCGGG AAAGCACCGG TTCCCTGGAC 
AATGACGATG AGGAGATGGT GACATTTGAG CTGAGCGAGA TGCTCCTCAT GCTGCTCGCT 
GCAGGAAATG ATGAAGAGGT GACAGATTCA GAGGATGAGT GGGACTTGTT TCACGAGGAG 
CTGGACGATT TCTATGAGAT TTACCTATAG