adcy6 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	adcy6
Entrez Gene ID	101077549
Full Name	adenylate cyclase 6
Synonyms	AC-VI
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029833874.1	XP_029689734.1	adenylate cyclase type 6 isoform X1
XM_011602723.1	XP_011601025.1	adenylate cyclase type 6

Pathways from BioSystems
Gene Ontology

The following adcy6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the adcy6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo63849	XM_029833874.1 Latest version!	Takifugu rubripes adenylate cyclase 6 (adcy6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo30464	XM_011602723.1 Latest version!	Takifugu rubripes adenylate cyclase 6 (adcy6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

adcy6 ( XM_029833874.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029689734.1 Takifugu rubripes adenylate cyclase 6 (adcy6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo63849
Clone ID Related Accession (Same CDS sequence)	XM_029833874.1
Accession Version	XM_029833874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	adenylate cyclase type 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042287.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCAGA ACCCCAACAG AATGTCCTGG TTCAGTGGTT TCCTAGTCGC CAGAGTGGAT 
GACCGAAAGA CCGCCTGGGG GGAGCGCAAT GGCAAGAAAC GGAAGGGAGG CTCATCTCTC 
TGCAACCCAC GTTACATGAG CTGCCTGCGG GACCCAGATG CTATGGAGCC CCCCTCTGGA 
GCCCCCCTCC ATCAGCACCA CCGCGGAGGG GTGACAGGGG CCATGGGGGG CGGGGGCAAC 
TTTGGAGTTC GATGCGGATG GCAAGAGGAC CACTACGGCA AAAGGGGCGG GGCTCTCGGG 
GAAGGGGTGG GGCTGAAGTC GGTCGACCTG GGCTTCGAAG ACGGGGATCT TAAAGGGCGG 
AAGGGGGGGT GCAACGGCGG GAGCGGGGAC ATGGGCGACG AGGGTCCCAA CGGCGCGGCC 
GGAGTGGTGA CGGCTGCAGA CTGCTGGCTC AGGGTGGTGC AGGTGTTCCA GTCCAAAAAA 
TTCCAGTCCC GCAAACTGGA GCGCCTGTAC CAGCGCTACT TCTTTAGGTT GAACCAGAGC 
TCGCTGACCA TGCTGATGGG GGTACTGGTG ATAGTGTGCG GCATCATGTT GACCTTCCAC 
TGTGTCCACG CTGCCCCTCA CGTGGCCTAT TCCTCAGCCC TGTCGGTAGC CATGGCGCTG 
TTCATGGCTC TCATGGTGGT GTGCAACCGC AATGGCTTCC ACCAGGACTA CATGTGGATG 
GTCAGTTACC TGGTGATCGG AGTCTTGGTG TCGGTGCAGG TGTTTGGGGT GCTGATGGTG 
GCGCCCAGGA GCGCCTCAGA GGGAATCTGG TGGTCCGTGT TCTTCATCTA CATCATCTAC 
ACTCTGCTGC CCGTGAGGAT GAGAGCGGCG GTGCTGAGCG GAGCCGTGCT GTCCGTCATA 
CATGTGGTCA CCACCTGGCA GATCAACCAG GAAGATGAGT TCTTGTGGAA ACAGGTGAGC 
GCTAATGTCC TGATCTTCCT GTGCACCAAC ATCATTGGAA TCTGTACCCA CTACCCTGCT 
GAGGTGTCCC AGAGACAGGC CTTCCAGGAG ACCCGAGGAT ACATCCAGGC CAGATTACAC 
CTGCAGAGAG AGAACCAACA GCAGGAGCGT CTGTTGCTGT CCGTGTTGCC AAGGCACGTC 
GCAATGGAGA TGAAGGCCGA CATCAACGCC AAGAAGGAAG ACATGATGTT CCACAAGATC 
TACATCCAGA AACACGACAA TGTCAGCATT TTGTTTGCCG ACATCGAGGG CTTCACCAGC 
CTGGCCTCCC AGTGCACGGC TCAGGAGCTG GTCATGACAC TCAATGAGCT CTTTGCACGT 
TTTGACAAGT TGGCGTCGGA AAACCACTGC CTGCGTATTA AGATTTTGGG TGATTGTTAT 
TACTGTGTGT CCGGACTGCC GGAGCCACGG GCCGACCACG CCCACTGCTG CGTGGAGATG 
GGCGTTGACA TGATTGAGGC CATATCGCTG GTGCGGGAGG TCACCGGGGT CAATGTTAAC 
ATGCGAGTGG GTATTCACAG TGGACGTGTG CACTGTGGCG TGCTGGGACT CAGGAAGTGG 
CAGTTTGATG TGTGGTCAAA CGACGTCACG CTGGCCAATC ACATGGAGGC AGGAGGCAAA 
GCAGGGCGGA TCCACATCAC AAAAGCCACT TTGGAGTATC TTAATGGGGA CTACGAGGTG 
GAACCGGGTT TTGGAGGCGA GAGGAACGCG TACCTGAAGG AGAACAGCAT CGAGACCTTC 
CTGGTCCTCG GCTGCAGTCA GAAACGGAAG GAGGAGAAGG CGATGATGGC GAAGATGCAG 
AGGACGAGAG CTAACTCTAA CGAGGGCTTG ATCCCACGCT GGGTCCCCGA CAGAAGCTTC 
TCCAGGACTA AAGATTCCAA AGTCTTTAGA CAAATGGGGA TCGATGAGAC CTCTAGTAAA 
GACAACCGTT GCGTTCAGGA GGCCATGAAC CCCGAGGACG AGGTGGATGA GTTTCTGGGG 
CGAGCCATCG ACGCCCGGAG CATCGACCAG CTGAGAAAAG ACCACGTTAA GAAGTTCCTG 
CTCACCTTCC AGACATCCAG CCTGGAGAAA AAGTATTCCA AGAAGGTGGA TGATCGTTTT 
GGAGGTTATG TTGCCTGCAC TCTCCTGGTA TTCTGCTTCA TATCATTCAT ACAGATTGTT 
ATCTTCCCAC ACACCCCGGT CATGCTGGGA ATCTACATCA GTATCTTCGT CATCCTTGCC 
AACATCCTCT TCATCTGTGC CATATATTCC TGCATCAAGC TGTTTCCGGC TGCCATGCAG 
ACGGTTTCAA AGAAGATCGT CCAGTCTCGC ACCAACAGCA CCCTGGTCGG AGTGTTTAGC 
ATCATCCTCG TTTTCATCTC TGCCTTTGTC AACATGTTTG CCTGCGACAC CACGAGCTTG 
GCGCAGTGCG TTGCTGAGAA GCTGAACACC TCTGCAGGCC TGGTGACGCC CTGTCTGATC 
CGCAGTTTGA ATGTGTCGGT CGAGGGGGGT CTAGAGATCT GTCCCGACCG AGGCCCCTCC 
TGCCCCTTCC CTGAGTATTT CAGCTACAGC GTGCTGCTGA CGCTGCTGGC CTGCTCAGTG 
TTCCTGCAGA TCAGCAGCAT CGGGAAGCTG GCCCTCATGG TGCTCATCCA GTTCAGCTTC 
CTGCTGCTGG TGGAGTGGCC ACAAGTAGCA CTGTTGGACA ATGCGGACCT CTTTGTCACC 
TCCAATGCCA TTGATTTAAA TGAAACTAGT GCTCAATGGT CCAGTTTCAA TGAAACTACA 
AACCAGTGCC CATTTGTCGT GACCAAAGTG TCCCTGAGGG TCATGACTCC AGTGATCCTC 
ACAGTTTTTG TCCTGGCACT ATACCTGCAC GCCCAGCAGG TCGAATCAAC CGCACGCCTC 
GACTTCCTGT GGAAATTACA GGCCACAGAA GAGAAGGAAG AGATGGAGGA ACTGCAGGCC 
TACAACCGTC GCCTCCTCCA TAATATTCTG CCTAAAGATG TAGCTGCTCA CTTCTTGGCG 
CGAGAGCGGC GCAATGACGA ACTATACTAC CAGTCGTGTG AGTGTGTGGC TGTAATGTTT 
GCCTCCATCA GCAATTTTTC TGAATTCTAT GTGGAGCTGG AAGCCAACAA CGAGGGAGTG 
GAGTGTCTCC GGCTGCTCAA CGAGATCATC GCCGACTTTG ACGAGATCAT CAGTGAGGAG 
AAGTTCAGGC AGCTAGAGAA GATCAAGACC ATTGGTTCCA CTTACATGGC AGCCTCTGGT 
CTCAATGACT CCACCTACGA TAAGGAGGGT CGTTCCCACA TTACTGCCCT GGCCGACTAC 
GCCATGAGAC TCAGAGAACA GATGAAATAC ATCAACGAGC ATTCCTTCAA CAACTTCCAG 
ATGAAAATAG GTCTGAACAT CGGGCCTGTG GTAGCAGGGG TCATCGGTGC ACGCAAGCCT 
CAATACGACA TCTGGGGAAA CACCGTTAAT GTGGCCAGTC GGATGGACAG CACAGGTGTA 
CCTGACCGCA TACAGGTGAC GACGGACCTC TACCAGGTGC TTGCAAACAA AGGCTACATC 
CTGGAGTGCC GCGGTGTGGT CAAGGTCAAA GGTAAAGGGG AAATGACAAC CTACTTCCTG 
AACGACGGGC CACCCAACAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029689734.1
CDS	370..3993
Translation	MYQNPNRMSWFSGFLVARVDDRKTAWGERNGKKRKGGSSLCNPRYMSCLRDPDAMEPPSGAPLHQHHRGGVTGAMGGGGNFGVRCGWQEDHYGKRGGALGEGVGLKSVDLGFEDGDLKGRKGGCNGGSGDMGDEGPNGAAGVVTAADCWLRVVQVFQSKKFQSRKLERLYQRYFFRLNQSSLTMLMGVLVIVCGIMLTFHCVHAAPHVAYSSALSVAMALFMALMVVCNRNGFHQDYMWMVSYLVIGVLVSVQVFGVLMVAPRSASEGIWWSVFFIYIIYTLLPVRMRAAVLSGAVLSVIHVVTTWQINQEDEFLWKQVSANVLIFLCTNIIGICTHYPAEVSQRQAFQETRGYIQARLHLQRENQQQERLLLSVLPRHVAMEMKADINAKKEDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQELVMTLNELFARFDKLASENHCLRIKILGDCYYCVSGLPEPRADHAHCCVEMGVDMIEAISLVREVTGVNVNMRVGIHSGRVHCGVLGLRKWQFDVWSNDVTLANHMEAGGKAGRIHITKATLEYLNGDYEVEPGFGGERNAYLKENSIETFLVLGCSQKRKEEKAMMAKMQRTRANSNEGLIPRWVPDRSFSRTKDSKVFRQMGIDETSSKDNRCVQEAMNPEDEVDEFLGRAIDARSIDQLRKDHVKKFLLTFQTSSLEKKYSKKVDDRFGGYVACTLLVFCFISFIQIVIFPHTPVMLGIYISIFVILANILFICAIYSCIKLFPAAMQTVSKKIVQSRTNSTLVGVFSIILVFISAFVNMFACDTTSLAQCVAEKLNTSAGLVTPCLIRSLNVSVEGGLEICPDRGPSCPFPEYFSYSVLLTLLACSVFLQISSIGKLALMVLIQFSFLLLVEWPQVALLDNADLFVTSNAIDLNETSAQWSSFNETTNQCPFVVTKVSLRVMTPVILTVFVLALYLHAQQVESTARLDFLWKLQATEEKEEMEELQAYNRRLLHNILPKDVAAHFLARERRNDELYYQSCECVAVMFASISNFSEFYVELEANNEGVECLRLLNEIIADFDEIISEEKFRQLEKIKTIGSTYMAASGLNDSTYDKEGRSHITALADYAMRLREQMKYINEHSFNNFQMKIGLNIGPVVAGVIGARKPQYDIWGNTVNVASRMDSTGVPDRIQVTTDLYQVLANKGYILECRGVVKVKGKGEMTTYFLNDGPPNS

Target ORF information:

RefSeq Version	XM_029833874.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes adenylate cyclase 6 (adcy6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029833874.1

ORF Insert Sequence:

ATGTACCAGA ACCCCAACAG AATGTCCTGG TTCAGTGGTT TCCTAGTCGC CAGAGTGGAT 
GACCGAAAGA CCGCCTGGGG GGAGCGCAAT GGCAAGAAAC GGAAGGGAGG CTCATCTCTC 
TGCAACCCAC GTTACATGAG CTGCCTGCGG GACCCAGATG CTATGGAGCC CCCCTCTGGA 
GCCCCCCTCC ATCAGCACCA CCGCGGAGGG GTGACAGGGG CCATGGGGGG CGGGGGCAAC 
TTTGGAGTTC GATGCGGATG GCAAGAGGAC CACTACGGCA AAAGGGGCGG GGCTCTCGGG 
GAAGGGGTGG GGCTGAAGTC GGTCGACCTG GGCTTCGAAG ACGGGGATCT TAAAGGGCGG 
AAGGGGGGGT GCAACGGCGG GAGCGGGGAC ATGGGCGACG AGGGTCCCAA CGGCGCGGCC 
GGAGTGGTGA CGGCTGCAGA CTGCTGGCTC AGGGTGGTGC AGGTGTTCCA GTCCAAAAAA 
TTCCAGTCCC GCAAACTGGA GCGCCTGTAC CAGCGCTACT TCTTTAGGTT GAACCAGAGC 
TCGCTGACCA TGCTGATGGG GGTACTGGTG ATAGTGTGCG GCATCATGTT GACCTTCCAC 
TGTGTCCACG CTGCCCCTCA CGTGGCCTAT TCCTCAGCCC TGTCGGTAGC CATGGCGCTG 
TTCATGGCTC TCATGGTGGT GTGCAACCGC AATGGCTTCC ACCAGGACTA CATGTGGATG 
GTCAGTTACC TGGTGATCGG AGTCTTGGTG TCGGTGCAGG TGTTTGGGGT GCTGATGGTG 
GCGCCCAGGA GCGCCTCAGA GGGAATCTGG TGGTCCGTGT TCTTCATCTA CATCATCTAC 
ACTCTGCTGC CCGTGAGGAT GAGAGCGGCG GTGCTGAGCG GAGCCGTGCT GTCCGTCATA 
CATGTGGTCA CCACCTGGCA GATCAACCAG GAAGATGAGT TCTTGTGGAA ACAGGTGAGC 
GCTAATGTCC TGATCTTCCT GTGCACCAAC ATCATTGGAA TCTGTACCCA CTACCCTGCT 
GAGGTGTCCC AGAGACAGGC CTTCCAGGAG ACCCGAGGAT ACATCCAGGC CAGATTACAC 
CTGCAGAGAG AGAACCAACA GCAGGAGCGT CTGTTGCTGT CCGTGTTGCC AAGGCACGTC 
GCAATGGAGA TGAAGGCCGA CATCAACGCC AAGAAGGAAG ACATGATGTT CCACAAGATC 
TACATCCAGA AACACGACAA TGTCAGCATT TTGTTTGCCG ACATCGAGGG CTTCACCAGC 
CTGGCCTCCC AGTGCACGGC TCAGGAGCTG GTCATGACAC TCAATGAGCT CTTTGCACGT 
TTTGACAAGT TGGCGTCGGA AAACCACTGC CTGCGTATTA AGATTTTGGG TGATTGTTAT 
TACTGTGTGT CCGGACTGCC GGAGCCACGG GCCGACCACG CCCACTGCTG CGTGGAGATG 
GGCGTTGACA TGATTGAGGC CATATCGCTG GTGCGGGAGG TCACCGGGGT CAATGTTAAC 
ATGCGAGTGG GTATTCACAG TGGACGTGTG CACTGTGGCG TGCTGGGACT CAGGAAGTGG 
CAGTTTGATG TGTGGTCAAA CGACGTCACG CTGGCCAATC ACATGGAGGC AGGAGGCAAA 
GCAGGGCGGA TCCACATCAC AAAAGCCACT TTGGAGTATC TTAATGGGGA CTACGAGGTG 
GAACCGGGTT TTGGAGGCGA GAGGAACGCG TACCTGAAGG AGAACAGCAT CGAGACCTTC 
CTGGTCCTCG GCTGCAGTCA GAAACGGAAG GAGGAGAAGG CGATGATGGC GAAGATGCAG 
AGGACGAGAG CTAACTCTAA CGAGGGCTTG ATCCCACGCT GGGTCCCCGA CAGAAGCTTC 
TCCAGGACTA AAGATTCCAA AGTCTTTAGA CAAATGGGGA TCGATGAGAC CTCTAGTAAA 
GACAACCGTT GCGTTCAGGA GGCCATGAAC CCCGAGGACG AGGTGGATGA GTTTCTGGGG 
CGAGCCATCG ACGCCCGGAG CATCGACCAG CTGAGAAAAG ACCACGTTAA GAAGTTCCTG 
CTCACCTTCC AGACATCCAG CCTGGAGAAA AAGTATTCCA AGAAGGTGGA TGATCGTTTT 
GGAGGTTATG TTGCCTGCAC TCTCCTGGTA TTCTGCTTCA TATCATTCAT ACAGATTGTT 
ATCTTCCCAC ACACCCCGGT CATGCTGGGA ATCTACATCA GTATCTTCGT CATCCTTGCC 
AACATCCTCT TCATCTGTGC CATATATTCC TGCATCAAGC TGTTTCCGGC TGCCATGCAG 
ACGGTTTCAA AGAAGATCGT CCAGTCTCGC ACCAACAGCA CCCTGGTCGG AGTGTTTAGC 
ATCATCCTCG TTTTCATCTC TGCCTTTGTC AACATGTTTG CCTGCGACAC CACGAGCTTG 
GCGCAGTGCG TTGCTGAGAA GCTGAACACC TCTGCAGGCC TGGTGACGCC CTGTCTGATC 
CGCAGTTTGA ATGTGTCGGT CGAGGGGGGT CTAGAGATCT GTCCCGACCG AGGCCCCTCC 
TGCCCCTTCC CTGAGTATTT CAGCTACAGC GTGCTGCTGA CGCTGCTGGC CTGCTCAGTG 
TTCCTGCAGA TCAGCAGCAT CGGGAAGCTG GCCCTCATGG TGCTCATCCA GTTCAGCTTC 
CTGCTGCTGG TGGAGTGGCC ACAAGTAGCA CTGTTGGACA ATGCGGACCT CTTTGTCACC 
TCCAATGCCA TTGATTTAAA TGAAACTAGT GCTCAATGGT CCAGTTTCAA TGAAACTACA 
AACCAGTGCC CATTTGTCGT GACCAAAGTG TCCCTGAGGG TCATGACTCC AGTGATCCTC 
ACAGTTTTTG TCCTGGCACT ATACCTGCAC GCCCAGCAGG TCGAATCAAC CGCACGCCTC 
GACTTCCTGT GGAAATTACA GGCCACAGAA GAGAAGGAAG AGATGGAGGA ACTGCAGGCC 
TACAACCGTC GCCTCCTCCA TAATATTCTG CCTAAAGATG TAGCTGCTCA CTTCTTGGCG 
CGAGAGCGGC GCAATGACGA ACTATACTAC CAGTCGTGTG AGTGTGTGGC TGTAATGTTT 
GCCTCCATCA GCAATTTTTC TGAATTCTAT GTGGAGCTGG AAGCCAACAA CGAGGGAGTG 
GAGTGTCTCC GGCTGCTCAA CGAGATCATC GCCGACTTTG ACGAGATCAT CAGTGAGGAG 
AAGTTCAGGC AGCTAGAGAA GATCAAGACC ATTGGTTCCA CTTACATGGC AGCCTCTGGT 
CTCAATGACT CCACCTACGA TAAGGAGGGT CGTTCCCACA TTACTGCCCT GGCCGACTAC 
GCCATGAGAC TCAGAGAACA GATGAAATAC ATCAACGAGC ATTCCTTCAA CAACTTCCAG 
ATGAAAATAG GTCTGAACAT CGGGCCTGTG GTAGCAGGGG TCATCGGTGC ACGCAAGCCT 
CAATACGACA TCTGGGGAAA CACCGTTAAT GTGGCCAGTC GGATGGACAG CACAGGTGTA 
CCTGACCGCA TACAGGTGAC GACGGACCTC TACCAGGTGC TTGCAAACAA AGGCTACATC 
CTGGAGTGCC GCGGTGTGGT CAAGGTCAAA GGTAAAGGGG AAATGACAAC CTACTTCCTG 
AACGACGGGC CACCCAACAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

adcy6 ( XM_011602723.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011601025.1 Takifugu rubripes adenylate cyclase 6 (adcy6), mRNA.

Price & Availability↑

CloneID	OTo30464
Clone ID Related Accession (Same CDS sequence)	XM_011602723.1
Accession Version	XM_011602723.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	adenylate cyclase type 6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070935.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCTGATGG GGGTACTGGT GATAGTGTGC GGCATCATGT TGACCTTCCA CTGTGTCCAC 
GCTGCCCCTC ACGTGGCCTA TTCCTCAGCC CTGTCGGTAG CCATGGCGCT GTTCATGGCT 
CTCATGGTGG TGTGCAACCG CAATGGCTTC CACCAGGACT ACATGTGGAT GGTCAGTTAC 
CTGGTGATCG GAGTCTTGGT GTCGGTGCAG GTGTTTGGGG TGCTGATGGT GGCGCCCAGG 
AGCGCCTCAG AGGGAATCTG GTGGTCCGTG TTCTTCATCT ACATCATCTA CACTCTGCTG 
CCCGTGAGGA TGAGAGCGGC GGTGCTGAGC GGAGCCGTGC TGTCCGTCAT ACATGTGGTC 
ACCACCTGGC AGATCAACCA GGAAGATGAG TTCTTGTGGA AACAGGTGAG CGCTAATGTC 
CTGATCTTCC TGTGCACCAA CATCATTGGA ATCTGTACCC ACTACCCTGC TGAGGTGTCC 
CAGAGACAGG CCTTCCAGGA GACCCGAGGA TACATCCAGG CCAGATTACA CCTGCAGAGA 
GAGAACCAAC AGCAGGAGCG TCTGTTGCTG TCCGTGTTGC CAAGGCACGT CGCAATGGAG 
ATGAAGGCCG ACATCAACGC CAAGAAGGAA GACATGATGT TCCACAAGAT CTACATCCAG 
AAACACGACA ATGTCAGCAT TTTGTTTGCC GACATCGAGG GCTTCACCAG CCTGGCCTCC 
CAGTGCACGG CTCAGGAGCT GGTCATGACA CTCAATGAGC TCTTTGCACG TTTTGACAAG 
TTGGCGTCGG AAAACCACTG CCTGCGTATT AAGATTTTGG GTGATTGTTA TTACTGTGTG 
TCCGGACTGC CGGAGCCACG GGCCGACCAC GCCCACTGCT GCGTGGAGAT GGGCGTTGAC 
ATGATTGAGG CCATATCGCT GGTGCGGGAG GTCACCGGGG TCAATGTTAA CATGCGAGTG 
GGTATTCACA GTGGACGTGT GCACTGTGGC GTGCTGGGAC TCAGGAAGTG GCAGTTTGAT 
GTGTGGTCAA ACGACGTCAC ACTGGCCAAT CACATGGAGG CAGGAGGCAA AGCAGGGCGG 
ATCCACATCA CAAAAGCCAC TTTGGAGTAT CTTAATGGGG ACTACGAGGT GGAACCGGGT 
TTTGGAGGCG AGAGGAACGC GTACCTGAAG GAGAACAGCA TCGAGACCTT CCTGGTCCTC 
GGCTGCAGTC AGAAACGGAA GGAGGAGAAG GCGATGATGG CGAAGATGCA GAGGACGAGA 
GCTAACTCTA ACGAGGGCTT GATCCCACGC TGGGTCCCCG ACAGAAGCTT CTCCAGGACT 
AAAGATTCCA AAGTCTTTAG ACAAATGGGG ATCGATGAGA CCTCTAGTAA AGACAACCGT 
TGCGTTCAGG AGGCCATGAA CCCCGAGGAC GAGGTGGATG AGTTTCTGGG GCGAGCCATC 
GACGCCCGGA GCATCGACCA GCTGAGAAAA GACCACGTTA AGAAGTTCCT GCTCACCTTC 
CAGACATCCA GCCTGGAGAA AAAGTATTCC AAGAAGGTGG ATGATCGTTT TGGAGGTTAT 
GTTGCCTGCA CTCTCCTGGT ATTCTGCTTC ATATCATTCA TACAGATTGT TATCTTCCCA 
CACACCCCGG TCATGCTGGG AATCTACATC AGTATCTTCG TCATCCTTGC CAACATCCTC 
TTCATCTGTG CCATATATTC CTGCATCAAG CTGTTTCCGG CTGCCATGCA GACGGTTTCA 
AAGAAGATCG TCCAGTCTCG CACCAACAGC ACCCTGGTCG GAGTGTTTAG CATCATCCTC 
GTTTTCATCT CTGCCTTTGT CAACATGTTT GCCTGCGACA CCACGAGCTT GGCGCAGTGC 
GTTGCTGAGA AGCTGAACAC CTCTGCAGGC CTGGTGACGC CCTGTCTGAT CCGCAGTTTG 
AATGTGTCGG TCGAGGGGGG TCTAGAGATC TGTCCCGACC GAGGCCCCTC CTGCCCCTTC 
CCTGAGTATT TCAGCTACAG CGTGCTGCTG ACGCTGCTGG CCTGCTCAGT GTTCCTGCAG 
ATCAGCAGCA TCGGGAAGCT GGCCCTCATG GTGCTCATCC AGTTCAGCTT CCTGCTGCTG 
GTGGAGTGGC CACAAGTAGC ACTGTTGGAC AATGCGGACC TCTTTGTCAC CTCCAATGCC 
ATTGATTTAA ATGAAACTAG TGCTCAATGG TCCAGTTTCA ATGAAACTAC AAACCAGTGC 
CCATTTGTCG TGACCAAAGT GTCCCTGAGG GTCATGACTC CAGTGATCCT CACAGTTTTT 
GTCCTGGCAC TATACCTGCA CGCCCAGCAG GTCGAATCAA CCGCACGCCT CGACTTCCTG 
TGGAAATTAC AGGCCACAGA AGAGAAGGAA GAGATGGAGG AACTGCAGGC CTACAACCGT 
CGCCTCCTCC ATAATATTCT GCCTAAAGAT GTAGCTGCTC ACTTCTTGGC GCGAGAGCGG 
CGCAATGACG AACTATACTA CCAGTCGTGT GAGTGTGTGG CTGTAATGTT TGCCTCCATC 
AGCAATTTTT CTGAATTCTA TGTGGAGCTG GAAGCCAACA ACGAGGGAGT GGAGTGTCTC 
CGGCTGCTCA ACGAGATCAT CGCCGACTTT GACGAGATCA TCAGTGAGGA GAAGTTCAGG 
CAGCTAGAGA AGATCAAGAC CATTGGTTCC ACTTACATGG CAGCCTCTGG TCTCAATGAC 
TCCACCTACG ATAAGGAGGG TCGTTCCCAC ATTACTGCCC TGGCCGACTA CGCCATGAGA 
CTCAGAGAAC AGATGAAATA CATCAACGAG CATTCCTTCA ACAACTTCCA GATGAAAATA 
GGTCTGAACA TCGGGCCTGT GGTAGCAGGG GTCATCGGTG CACGCAAGCC TCAATACGAC 
ATCTGGGGAA ACACCGTTAA TGTGGCCAGT CGGATGGACA GCACAGGTGT ACCTGACCGC 
ATACAGGTGG GGTTCAAGCG ACTGTTGATG GTTAATGATA AAGCTTTAGA GATTAGAAGA 
GTGACGACGG ACCTCTACCA GGTGCTTGCA AACAAAGGCT ACATCCTGGA GTGCCGCGGT 
GTGGTCAAGG TCAAAGGTAA AGGGGAAATG ACAACCTACT TCCTGAACGA CGGGCCACCC 
AACAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601025.1
CDS	74..3202
Translation	MLMGVLVIVCGIMLTFHCVHAAPHVAYSSALSVAMALFMALMVVCNRNGFHQDYMWMVSYLVIGVLVSVQVFGVLMVAPRSASEGIWWSVFFIYIIYTLLPVRMRAAVLSGAVLSVIHVVTTWQINQEDEFLWKQVSANVLIFLCTNIIGICTHYPAEVSQRQAFQETRGYIQARLHLQRENQQQERLLLSVLPRHVAMEMKADINAKKEDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQELVMTLNELFARFDKLASENHCLRIKILGDCYYCVSGLPEPRADHAHCCVEMGVDMIEAISLVREVTGVNVNMRVGIHSGRVHCGVLGLRKWQFDVWSNDVTLANHMEAGGKAGRIHITKATLEYLNGDYEVEPGFGGERNAYLKENSIETFLVLGCSQKRKEEKAMMAKMQRTRANSNEGLIPRWVPDRSFSRTKDSKVFRQMGIDETSSKDNRCVQEAMNPEDEVDEFLGRAIDARSIDQLRKDHVKKFLLTFQTSSLEKKYSKKVDDRFGGYVACTLLVFCFISFIQIVIFPHTPVMLGIYISIFVILANILFICAIYSCIKLFPAAMQTVSKKIVQSRTNSTLVGVFSIILVFISAFVNMFACDTTSLAQCVAEKLNTSAGLVTPCLIRSLNVSVEGGLEICPDRGPSCPFPEYFSYSVLLTLLACSVFLQISSIGKLALMVLIQFSFLLLVEWPQVALLDNADLFVTSNAIDLNETSAQWSSFNETTNQCPFVVTKVSLRVMTPVILTVFVLALYLHAQQVESTARLDFLWKLQATEEKEEMEELQAYNRRLLHNILPKDVAAHFLARERRNDELYYQSCECVAVMFASISNFSEFYVELEANNEGVECLRLLNEIIADFDEIISEEKFRQLEKIKTIGSTYMAASGLNDSTYDKEGRSHITALADYAMRLREQMKYINEHSFNNFQMKIGLNIGPVVAGVIGARKPQYDIWGNTVNVASRMDSTGVPDRIQVGFKRLLMVNDKALEIRRVTTDLYQVLANKGYILECRGVVKVKGKGEMTTYFLNDGPPNS

Target ORF information:

RefSeq Version	XM_011602723.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes adenylate cyclase 6 (adcy6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602723.1

ORF Insert Sequence:

ATGCTGATGG GGGTACTGGT GATAGTGTGC GGCATCATGT TGACCTTCCA CTGTGTCCAC 
GCTGCCCCTC ACGTGGCCTA TTCCTCAGCC CTGTCGGTAG CCATGGCGCT GTTCATGGCT 
CTCATGGTGG TGTGCAACCG CAATGGCTTC CACCAGGACT ACATGTGGAT GGTCAGTTAC 
CTGGTGATCG GAGTCTTGGT GTCGGTGCAG GTGTTTGGGG TGCTGATGGT GGCGCCCAGG 
AGCGCCTCAG AGGGAATCTG GTGGTCCGTG TTCTTCATCT ACATCATCTA CACTCTGCTG 
CCCGTGAGGA TGAGAGCGGC GGTGCTGAGC GGAGCCGTGC TGTCCGTCAT ACATGTGGTC 
ACCACCTGGC AGATCAACCA GGAAGATGAG TTCTTGTGGA AACAGGTGAG CGCTAATGTC 
CTGATCTTCC TGTGCACCAA CATCATTGGA ATCTGTACCC ACTACCCTGC TGAGGTGTCC 
CAGAGACAGG CCTTCCAGGA GACCCGAGGA TACATCCAGG CCAGATTACA CCTGCAGAGA 
GAGAACCAAC AGCAGGAGCG TCTGTTGCTG TCCGTGTTGC CAAGGCACGT CGCAATGGAG 
ATGAAGGCCG ACATCAACGC CAAGAAGGAA GACATGATGT TCCACAAGAT CTACATCCAG 
AAACACGACA ATGTCAGCAT TTTGTTTGCC GACATCGAGG GCTTCACCAG CCTGGCCTCC 
CAGTGCACGG CTCAGGAGCT GGTCATGACA CTCAATGAGC TCTTTGCACG TTTTGACAAG 
TTGGCGTCGG AAAACCACTG CCTGCGTATT AAGATTTTGG GTGATTGTTA TTACTGTGTG 
TCCGGACTGC CGGAGCCACG GGCCGACCAC GCCCACTGCT GCGTGGAGAT GGGCGTTGAC 
ATGATTGAGG CCATATCGCT GGTGCGGGAG GTCACCGGGG TCAATGTTAA CATGCGAGTG 
GGTATTCACA GTGGACGTGT GCACTGTGGC GTGCTGGGAC TCAGGAAGTG GCAGTTTGAT 
GTGTGGTCAA ACGACGTCAC ACTGGCCAAT CACATGGAGG CAGGAGGCAA AGCAGGGCGG 
ATCCACATCA CAAAAGCCAC TTTGGAGTAT CTTAATGGGG ACTACGAGGT GGAACCGGGT 
TTTGGAGGCG AGAGGAACGC GTACCTGAAG GAGAACAGCA TCGAGACCTT CCTGGTCCTC 
GGCTGCAGTC AGAAACGGAA GGAGGAGAAG GCGATGATGG CGAAGATGCA GAGGACGAGA 
GCTAACTCTA ACGAGGGCTT GATCCCACGC TGGGTCCCCG ACAGAAGCTT CTCCAGGACT 
AAAGATTCCA AAGTCTTTAG ACAAATGGGG ATCGATGAGA CCTCTAGTAA AGACAACCGT 
TGCGTTCAGG AGGCCATGAA CCCCGAGGAC GAGGTGGATG AGTTTCTGGG GCGAGCCATC 
GACGCCCGGA GCATCGACCA GCTGAGAAAA GACCACGTTA AGAAGTTCCT GCTCACCTTC 
CAGACATCCA GCCTGGAGAA AAAGTATTCC AAGAAGGTGG ATGATCGTTT TGGAGGTTAT 
GTTGCCTGCA CTCTCCTGGT ATTCTGCTTC ATATCATTCA TACAGATTGT TATCTTCCCA 
CACACCCCGG TCATGCTGGG AATCTACATC AGTATCTTCG TCATCCTTGC CAACATCCTC 
TTCATCTGTG CCATATATTC CTGCATCAAG CTGTTTCCGG CTGCCATGCA GACGGTTTCA 
AAGAAGATCG TCCAGTCTCG CACCAACAGC ACCCTGGTCG GAGTGTTTAG CATCATCCTC 
GTTTTCATCT CTGCCTTTGT CAACATGTTT GCCTGCGACA CCACGAGCTT GGCGCAGTGC 
GTTGCTGAGA AGCTGAACAC CTCTGCAGGC CTGGTGACGC CCTGTCTGAT CCGCAGTTTG 
AATGTGTCGG TCGAGGGGGG TCTAGAGATC TGTCCCGACC GAGGCCCCTC CTGCCCCTTC 
CCTGAGTATT TCAGCTACAG CGTGCTGCTG ACGCTGCTGG CCTGCTCAGT GTTCCTGCAG 
ATCAGCAGCA TCGGGAAGCT GGCCCTCATG GTGCTCATCC AGTTCAGCTT CCTGCTGCTG 
GTGGAGTGGC CACAAGTAGC ACTGTTGGAC AATGCGGACC TCTTTGTCAC CTCCAATGCC 
ATTGATTTAA ATGAAACTAG TGCTCAATGG TCCAGTTTCA ATGAAACTAC AAACCAGTGC 
CCATTTGTCG TGACCAAAGT GTCCCTGAGG GTCATGACTC CAGTGATCCT CACAGTTTTT 
GTCCTGGCAC TATACCTGCA CGCCCAGCAG GTCGAATCAA CCGCACGCCT CGACTTCCTG 
TGGAAATTAC AGGCCACAGA AGAGAAGGAA GAGATGGAGG AACTGCAGGC CTACAACCGT 
CGCCTCCTCC ATAATATTCT GCCTAAAGAT GTAGCTGCTC ACTTCTTGGC GCGAGAGCGG 
CGCAATGACG AACTATACTA CCAGTCGTGT GAGTGTGTGG CTGTAATGTT TGCCTCCATC 
AGCAATTTTT CTGAATTCTA TGTGGAGCTG GAAGCCAACA ACGAGGGAGT GGAGTGTCTC 
CGGCTGCTCA ACGAGATCAT CGCCGACTTT GACGAGATCA TCAGTGAGGA GAAGTTCAGG 
CAGCTAGAGA AGATCAAGAC CATTGGTTCC ACTTACATGG CAGCCTCTGG TCTCAATGAC 
TCCACCTACG ATAAGGAGGG TCGTTCCCAC ATTACTGCCC TGGCCGACTA CGCCATGAGA 
CTCAGAGAAC AGATGAAATA CATCAACGAG CATTCCTTCA ACAACTTCCA GATGAAAATA 
GGTCTGAACA TCGGGCCTGT GGTAGCAGGG GTCATCGGTG CACGCAAGCC TCAATACGAC 
ATCTGGGGAA ACACCGTTAA TGTGGCCAGT CGGATGGACA GCACAGGTGT ACCTGACCGC 
ATACAGGTGG GGTTCAAGCG ACTGTTGATG GTTAATGATA AAGCTTTAGA GATTAGAAGA 
GTGACGACGG ACCTCTACCA GGTGCTTGCA AACAAAGGCT ACATCCTGGA GTGCCGCGGT 
GTGGTCAAGG TCAAAGGTAA AGGGGAAATG ACAACCTACT TCCTGAACGA CGGGCCACCC 
AACAGTTAG