LOC101075949 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101075949
Entrez Gene ID	101075949
Full Name	heat shock protein family A (Hsp70) member 4 like
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003962662.2	XP_003962711.1	heat shock 70 kDa protein 4L
XM_003962662.3	XP_003962711.2	heat shock 70 kDa protein 4L

Pathways from BioSystems
Gene Ontology

The following LOC101075949 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101075949 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo01692	XM_003962662.2 Latest version!	Takifugu rubripes heat shock 70kDa protein 4-like (hspa4l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTo73418	XM_003962662.3 Latest version!	Takifugu rubripes heat shock 70 kDa protein 4L (LOC101075949), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101075949 ( XM_003962662.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003962711.1 Takifugu rubripes heat shock 70kDa protein 4-like (hspa4l), mRNA.

Price & Availability↑

CloneID	OTo01692
Clone ID Related Accession (Same CDS sequence)	XM_003962662.2
Accession Version	XM_003962662.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	heat shock 70 kDa protein 4L
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070921.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003962662.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGTGG TCGGGATTGA TGTGGGTTTC CAAAGCTGTT ACATCGCTGT GGCCAGGAGC 
GGCGGCATTG AAACCATTGC CAATGAATAC AGTGACAGAT GCACACCAGC TTGTGTGTCC 
TTGGCAACTA AAAACCGTAT AATTGGAAAC GCTGCCAAAA GTCAGATCAT AACAAACTTC 
AAAAATACAG TCCATGGCTT CAAAAAGTTC CACGGCAGGA CGTTCGATGA CCCATTCGTC 
CAAGCAGAAA AGCCCAAACT GCCTTACAGC TTGCATAAAC TGGCCAATGG GAGCACTGGA 
ATTCAGGTGC GATATTTGGA CGACGACAAA GTGTTCACAG TTGAGCAGAT CACGGGGATG 
CTGCTCAGCA AGCTGAAAGA GACGTCTGAG GGGGCTCTGA AGAAGCCCGT GGTGGACTGC 
GTCATCTCCG TCCCAAGTTT TTTCACAGAC GCTGAAAGAC GTTCGGTGTT TGACGCTTCT 
CAAATTGCAG GGCTGAACTG TCTTCGGCTA ATTAACGACA CTACTGCAGT TGCCTTGGCC 
TACGGCATCT ACAAACAGGA CCTTCCTGCT CCAGAAGAGA GGCCAAGAAA CGTTGTGTTT 
GTGGACATGG GACATTCATC ATTCCAGGTC TCCATCGCTG CCTTCAACAA AGGCAAACTC 
AAGGTCCTCG CCACTGCGTT CGACCCATAC CTCGGTGGGC GCAACCTCGA CGAGGTGTTG 
GTCGATTACT TCTGCGAGGA GTTCAAGGGC AAGTACAAAC TCAATGTAAG GGAGAACCCG 
AGGGCTCTGC TGCGGCTGCA CCAGGAGTGC GAGAAGCTGA AGAAGCTGAT GAGCGCCAAC 
TCCTCCAACC TGCCTCTGAA CATCGAGTGC TTCATGAATG ACATCGACGT GTCCAGCAGG 
ATGAACAGAG GCCAATTTGA AGAAATGTCT TCTCAGTATC TGATGAGAGT GGAGGCGCCT 
TTAAGAGCCG TCCTCGAACA GTCGAAGCTA AGCCGAGATG ACGTCTACGC CGTGGAGATA 
GTTGGAGGAG CGACAAGAAT CCCGGCGATC AAAGAGCGAA TCAGCCGTTT CTTCTGCAAA 
GACATCAGCA CAACCCTCAA CGCAGATGAA GCCGTCGCTC GAGGCTGCGC ACTGCAGTGC 
GCCATTTTGT CGCCAGCGTT CAAGGTCCGC GAGTTCTCCA TCACCGACGT CGTTCCCTTT 
CCTATTACTC TCCGCTGGAA ATCTACAACA GAGGATGGAG TGGGAGAGTG TGAGGTGTTC 
TCGAAGAATC ACGCTGCTCC ATTTTCCAAA GTCATCACCT TTCACAAGAA GGAACCATTT 
GACCTTGAAG CCTTTTACAG TAATTCTCAA GAGCTTCCAT ACCCAGACCA CACCATAGGA 
TGCTTCTCTG TCCAGAACGT TTCTCCCCAG CCAGACGGAG ACAGCTCCAA AGTGAAGGTC 
AAGGTGCGGG TGAACATCCA TGGCATCTTC AGCGTGTCCA GCGCCTCTCT GATAGAGAAG 
CAGAAAGGAG AAGTGGAGGA CATGCAGATT GACTCTGAGC CACTGGTGCA GAATGAGGGG 
AGGGTGGAGG AGCAGGCTAA AATGCAGGTG GACCAGGAAG GTCAAGGTCA GGGGGAGGAA 
CAGGCCGATG AAAACAGCCC CAGCAATAAA GAGGGGCCAT CTGTGGAAAA ACAGGACCCA 
GCAGCAGGGG GGAGCAAGCC CAAGGTCAAG GTGAAGAGCA TTGATCTGCC GGTTGTGATG 
AACAGCATTA GACAGCTGGA CACATCTGTC CTAAATGACT TTGTGGAGTA TGAGCGTCAG 
ATGATTATTC AGGACAAACT AGTGAAGGAG CAGAATGATG CTAAAAATGC TGTTGAGGAG 
TACGTGTACG ATCTGCGGGA CAAACTCGGA GGCGTCTACG AAAAGTACAT CACCGAGAAT 
GACAGTAAAC AGTTGACACT GATGCTGGAG GAAACCGAGA ACTGGCTGTA TGAGGATGGA 
GAGGACCAGC CCAAACGAGT GTATGAGGAG AAGTTGGAGG CCCTCAAGAG GTTGGGTCAG 
CCCATTCAGG ACCGGCACAG AGAGCACGAG GACAGGCCGA GGGCTTTTGA AGAGCTGGGA 
AAGAAGCTGC AAGTCTACAT GAAGTTTTTG GATTCCTTTA AGCAGAAGGA TGAGCGATTC 
ATGCATTTAA ATGTGGAAGA AATGGGCACA GTGGAGAAAT GTGTGAGTGA CAGCATGGGC 
TGGATGAACA GCAAAATGAA TGCACAGAGC AAGCTGAGCA TTACTCAAGA CCCCGCTGTT 
AAAGTAGCAG ACATCATCGC TAAAATACAG GAGCTAGAGG ACGCGTGTCA TCCAGTGATC 
AACAGACCCA AGCCCAGTGC GGAGGAGACC TCTGAGGTGA ATGAGCAAAG CAGTGGAGTG 
CACGCAGCCA CCCAGGCTAA AGAAGGAAAG GGAGAAACTA AGGGAAACCA GCAGACCAAA 
GAGATGCAGA TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003962711.1
CDS	237..2714
Translation	MSVVGIDVGFQSCYIAVARSGGIETIANEYSDRCTPACVSLATKNRIIGNAAKSQIITNFKNTVHGFKKFHGRTFDDPFVQAEKPKLPYSLHKLANGSTGIQVRYLDDDKVFTVEQITGMLLSKLKETSEGALKKPVVDCVISVPSFFTDAERRSVFDASQIAGLNCLRLINDTTAVALAYGIYKQDLPAPEERPRNVVFVDMGHSSFQVSIAAFNKGKLKVLATAFDPYLGGRNLDEVLVDYFCEEFKGKYKLNVRENPRALLRLHQECEKLKKLMSANSSNLPLNIECFMNDIDVSSRMNRGQFEEMSSQYLMRVEAPLRAVLEQSKLSRDDVYAVEIVGGATRIPAIKERISRFFCKDISTTLNADEAVARGCALQCAILSPAFKVREFSITDVVPFPITLRWKSTTEDGVGECEVFSKNHAAPFSKVITFHKKEPFDLEAFYSNSQELPYPDHTIGCFSVQNVSPQPDGDSSKVKVKVRVNIHGIFSVSSASLIEKQKGEVEDMQIDSEPLVQNEGRVEEQAKMQVDQEGQGQGEEQADENSPSNKEGPSVEKQDPAAGGSKPKVKVKSIDLPVVMNSIRQLDTSVLNDFVEYERQMIIQDKLVKEQNDAKNAVEEYVYDLRDKLGGVYEKYITENDSKQLTLMLEETENWLYEDGEDQPKRVYEEKLEALKRLGQPIQDRHREHEDRPRAFEELGKKLQVYMKFLDSFKQKDERFMHLNVEEMGTVEKCVSDSMGWMNSKMNAQSKLSITQDPAVKVADIIAKIQELEDACHPVINRPKPSAEETSEVNEQSSGVHAATQAKEGKGETKGNQQTKEMQMD

Target ORF information:

RefSeq Version	XM_003962662.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes heat shock 70kDa protein 4-like (hspa4l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003962662.2

ORF Insert Sequence:

ATGTCAGTGG TCGGGATTGA TGTGGGTTTC CAAAGCTGTT ACATCGCTGT GGCCAGGAGC 
GGCGGCATTG AAACCATTGC CAATGAATAC AGTGACAGAT GCACACCAGC TTGTGTGTCC 
TTGGCAACTA AAAACCGTAT AATTGGAAAC GCTGCCAAAA GTCAGATCAT AACAAACTTC 
AAAAATACAG TCCATGGCTT CAAAAAGTTC CACGGCAGGA CGTTCGATGA CCCATTCGTC 
CAAGCAGAAA AGCCCAAACT GCCTTACAGC TTGCATAAAC TGGCCAATGG GAGCACTGGA 
ATTCAGGTGC GATATTTGGA CGACGACAAA GTGTTCACAG TTGAGCAGAT CACGGGGATG 
CTGCTCAGCA AGCTGAAAGA GACGTCTGAG GGGGCTCTGA AGAAGCCCGT GGTGGACTGC 
GTCATCTCCG TCCCAAGTTT TTTCACAGAC GCTGAAAGAC GTTCGGTGTT TGACGCTTCT 
CAAATTGCAG GGCTGAACTG TCTTCGGCTA ATTAACGACA CTACTGCAGT TGCCTTGGCC 
TACGGCATCT ACAAACAGGA CCTTCCTGCT CCAGAAGAGA GGCCAAGAAA CGTTGTGTTT 
GTGGACATGG GACATTCATC ATTCCAGGTC TCCATCGCTG CCTTCAACAA AGGCAAACTC 
AAGGTCCTCG CCACTGCGTT CGACCCATAC CTCGGTGGGC GCAACCTCGA CGAGGTGTTG 
GTCGATTACT TCTGCGAGGA GTTCAAGGGC AAGTACAAAC TCAATGTAAG GGAGAACCCG 
AGGGCTCTGC TGCGGCTGCA CCAGGAGTGC GAGAAGCTGA AGAAGCTGAT GAGCGCCAAC 
TCCTCCAACC TGCCTCTGAA CATCGAGTGC TTCATGAATG ACATCGACGT GTCCAGCAGG 
ATGAACAGAG GCCAATTTGA AGAAATGTCT TCTCAGTATC TGATGAGAGT GGAGGCGCCT 
TTAAGAGCCG TCCTCGAACA GTCGAAGCTA AGCCGAGATG ACGTCTACGC CGTGGAGATA 
GTTGGAGGAG CGACAAGAAT CCCGGCGATC AAAGAGCGAA TCAGCCGTTT CTTCTGCAAA 
GACATCAGCA CAACCCTCAA CGCAGATGAA GCCGTCGCTC GAGGCTGCGC ACTGCAGTGC 
GCCATTTTGT CGCCAGCGTT CAAGGTCCGC GAGTTCTCCA TCACCGACGT CGTTCCCTTT 
CCTATTACTC TCCGCTGGAA ATCTACAACA GAGGATGGAG TGGGAGAGTG TGAGGTGTTC 
TCGAAGAATC ACGCTGCTCC ATTTTCCAAA GTCATCACCT TTCACAAGAA GGAACCATTT 
GACCTTGAAG CCTTTTACAG TAATTCTCAA GAGCTTCCAT ACCCAGACCA CACCATAGGA 
TGCTTCTCTG TCCAGAACGT TTCTCCCCAG CCAGACGGAG ACAGCTCCAA AGTGAAGGTC 
AAGGTGCGGG TGAACATCCA TGGCATCTTC AGCGTGTCCA GCGCCTCTCT GATAGAGAAG 
CAGAAAGGAG AAGTGGAGGA CATGCAGATT GACTCTGAGC CACTGGTGCA GAATGAGGGG 
AGGGTGGAGG AGCAGGCTAA AATGCAGGTG GACCAGGAAG GTCAAGGTCA GGGGGAGGAA 
CAGGCCGATG AAAACAGCCC CAGCAATAAA GAGGGGCCAT CTGTGGAAAA ACAGGACCCA 
GCAGCAGGGG GGAGCAAGCC CAAGGTCAAG GTGAAGAGCA TTGATCTGCC GGTTGTGATG 
AACAGCATTA GACAGCTGGA CACATCTGTC CTAAATGACT TTGTGGAGTA TGAGCGTCAG 
ATGATTATTC AGGACAAACT AGTGAAGGAG CAGAATGATG CTAAAAATGC TGTTGAGGAG 
TACGTGTACG ATCTGCGGGA CAAACTCGGA GGCGTCTACG AAAAGTACAT CACCGAGAAT 
GACAGTAAAC AGTTGACACT GATGCTGGAG GAAACCGAGA ACTGGCTGTA TGAGGATGGA 
GAGGACCAGC CCAAACGAGT GTATGAGGAG AAGTTGGAGG CCCTCAAGAG GTTGGGTCAG 
CCCATTCAGG ACCGGCACAG AGAGCACGAG GACAGGCCGA GGGCTTTTGA AGAGCTGGGA 
AAGAAGCTGC AAGTCTACAT GAAGTTTTTG GATTCCTTTA AGCAGAAGGA TGAGCGATTC 
ATGCATTTAA ATGTGGAAGA AATGGGCACA GTGGAGAAAT GTGTGAGTGA CAGCATGGGC 
TGGATGAACA GCAAAATGAA TGCACAGAGC AAGCTGAGCA TTACTCAAGA CCCCGCTGTT 
AAAGTAGCAG ACATCATCGC TAAAATACAG GAGCTAGAGG ACGCGTGTCA TCCAGTGATC 
AACAGACCCA AGCCCAGTGC GGAGGAGACC TCTGAGGTGA ATGAGCAAAG CAGTGGAGTG 
CACGCAGCCA CCCAGGCTAA AGAAGGAAAG GGAGAAACTA AGGGAAACCA GCAGACCAAA 
GAGATGCAGA TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101075949 ( XM_003962662.3 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003962711.2 Takifugu rubripes heat shock 70 kDa protein 4L (LOC101075949), mRNA.

Price & Availability↑

CloneID	OTo73418
Clone ID Related Accession (Same CDS sequence)	XM_003962662.3
Accession Version	XM_003962662.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	heat shock 70 kDa protein 4L
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003962662.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGTGG TCGGGATTGA TGTGGGTTTC CAAAGCTGTT ACATCGCTGT GGCCAGGAGC 
GGCGGCATTG AAACCATTGC CAATGAATAC AGTGACAGAT GCACACCAGC TTGTGTGTCC 
TTGGCAACTA AAAACCGTAT AATTGGAAAC GCTGCCAAAA GTCAGATCAT AACAAACTTC 
AAAAATACAG TCCATGGCTT TAAAAAGTTC CACGGCAGGA CGTTCGATGA CCCATTCGTC 
CAAGCAGAAA AGCCCAAACT GCCTTACAGC TTGCATAAAC TGGCCAATGG GAGCACTGGA 
ATTCAGGTGC GATATTTGGA CGACGACAAA GTGTTCACAG TTGAGCAGAT CACGGGGATG 
CTGCTCAGCA AGCTGAAAGA GACGTCTGAG GGGGCTCTGA AGAAGCCCGT GGTGGACTGC 
GTCATCTCCG TCCCAAGTTT TTTCACAGAC GCTGAAAGAC GTTCGGTGTT TGACGCTTCT 
CAAATTGCAG GGCTGAACTG TCTTCGGCTA ATTAACGACA CTACTGCAGT TGCCTTGGCC 
TACGGCATCT ACAAACAGGA CCTTCCTGCT CCAGAAGAGA GGCCAAGAAA CGTTGTGTTT 
GTGGACATGG GACATTCATC ATTCCAGGTC TCCATCGCTG CCTTCAACAA AGGCAAACTC 
AAGGTCCTCG CCACTGCGTT CGACCCATAC CTCGGTGGGC GCAACCTCGA CGAGGTGTTG 
GTCGATTACT TCTGCGAGGA GTTCAAGGGC AAGTACAAAC TCAATGTAAG GGAGAACCCG 
AGGGCTCTGC TGCGGCTGCA CCAGGAGTGC GAGAAGCTGA AGAAGCTGAT GAGCGCCAAC 
TCCTCCAACC TGCCTCTGAA CATCGAGTGC TTCATGAATG ACATCGACGT GTCCAGCAGG 
ATGAACAGAG GCCAATTTGA AGAAATGTCT TCTCAGTATC TGATGAGAGT GGAGGCGCCT 
TTAAGAGCCG TCCTCGAACA GTCGAAGCTA AGCCGAGATG ACGTCTACGC CGTGGAGATA 
GTTGGAGGAG CGACAAGAAT CCCGGCGATC AAAGAGCGAA TCAGCCGTTT CTTCTGCAAA 
GACATCAGCA CAACCCTCAA CGCAGATGAA GCCGTCGCTC GAGGCTGCGC ACTGCAGTGC 
GCCATTTTGT CGCCAGCGTT CAAGGTCCGC GAGTTCTCCA TCACCGACGT CGTTCCCTTT 
CCTATTACTC TCCGCTGGAA ATCTACAACA GAGGATGGAG TGGGAGAGTG TGAGGTGTTC 
TCGAAGAATC ACGCTGCTCC ATTTTCCAAA GTCATCACCT TTCACAAGAA GGAACCATTT 
GACCTTGAAG CCTTTTACAG TAATTCTCAA GAGCTTCCAT ACCCAGACCA CACCATAGGA 
TGCTTCTCTG TCCAGAACGT TTCTCCCCAG CCAGACGGAG ACAGCTCCAA AGTGAAGGTC 
AAGGTGCGGG TGAACATCCA TGGCATCTTC AGCGTGTCCA GCGCCTCTCT GATAGAGAAG 
CAGAAAGGAG AAGTGGAGGA CATGCAGATT GACTCTGAGC CACTGGTGCA GAATGAGGGG 
AGGGTGGAGG AGCAGGCTAA AATGCAGGTG GACCAGCAAG GTCAAGGTCA GGGGGAGGAA 
CAGGCCGATG AAAACAGCCC CAGCAATAAA GAGGGGCCAT CTGTGGAAAA ACAGGACCCA 
GCAGCAGGGG GGAGCAAGCC CAAGGTCAAG GTGAAGAGCA TTGATCTGCC GGTTGTGATG 
AACAGCATTA GACAGCTGGA CACATCTGTC CTAAATGACT TTGTGGAGTA TGAGCGTCAG 
ATGATTATTC AGGACAAACT AGTGAAGGAG CAGAATGATG CTAAAAATGC TGTTGAGGAG 
TACGTGTACG ATCTGCGGGA CAAACTCGGA GGCGTCTACG AAAAGTACAT CACCGAGAAT 
GACAGTAAAC AGTTGACACT GATGCTGGAG GAAACCGAGA ACTGGCTGTA TGAGGATGGA 
GAGGACCAGC CCAAACGAGT GTATGAGGAG AAGTTGGAGG CCCTCAAGAG GTTGGGTCAG 
CCCATTCAGG ACCGGCACAG AGAGCACGAG GACAGGCCGA GGGCTTTTGA AGAGCTGGGA 
AAGAAGCTGC AAGTCTACAT GAAGTTTTTG GATTCCTTTA AGCAGAAGGA TGAGCGATTC 
ATGCATTTAA ATGTGGAAGA AATGGGCACA GTGGAGAAAT GTGTGAGTGA CAGCATGGGC 
TGGATGAACA GCAAAATGAA TGCACAGAGC AAGCTGAGCA TTACTCAAGA CCCCGCTGTT 
AAAGTAGCAG ACATCATCGC TAAAATACAG GAGCTAGAGG ACGCGTGTCA TCCAGTGATC 
AACAGACCCA AGCCCAGTGC GGAGGAGACC TCTGAGGTGA ATGAGCAAAG CAGTGGAGTG 
CACGCAGCCA CCCAGGCTAA AGAAGGAAAG GGAGAAACTA AGGGAAACCA GCAGACCAAA 
GAGATGCAGA TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003962711.2
CDS	301..2778
Translation	MSVVGIDVGFQSCYIAVARSGGIETIANEYSDRCTPACVSLATKNRIIGNAAKSQIITNFKNTVHGFKKFHGRTFDDPFVQAEKPKLPYSLHKLANGSTGIQVRYLDDDKVFTVEQITGMLLSKLKETSEGALKKPVVDCVISVPSFFTDAERRSVFDASQIAGLNCLRLINDTTAVALAYGIYKQDLPAPEERPRNVVFVDMGHSSFQVSIAAFNKGKLKVLATAFDPYLGGRNLDEVLVDYFCEEFKGKYKLNVRENPRALLRLHQECEKLKKLMSANSSNLPLNIECFMNDIDVSSRMNRGQFEEMSSQYLMRVEAPLRAVLEQSKLSRDDVYAVEIVGGATRIPAIKERISRFFCKDISTTLNADEAVARGCALQCAILSPAFKVREFSITDVVPFPITLRWKSTTEDGVGECEVFSKNHAAPFSKVITFHKKEPFDLEAFYSNSQELPYPDHTIGCFSVQNVSPQPDGDSSKVKVKVRVNIHGIFSVSSASLIEKQKGEVEDMQIDSEPLVQNEGRVEEQAKMQVDQQGQGQGEEQADENSPSNKEGPSVEKQDPAAGGSKPKVKVKSIDLPVVMNSIRQLDTSVLNDFVEYERQMIIQDKLVKEQNDAKNAVEEYVYDLRDKLGGVYEKYITENDSKQLTLMLEETENWLYEDGEDQPKRVYEEKLEALKRLGQPIQDRHREHEDRPRAFEELGKKLQVYMKFLDSFKQKDERFMHLNVEEMGTVEKCVSDSMGWMNSKMNAQSKLSITQDPAVKVADIIAKIQELEDACHPVINRPKPSAEETSEVNEQSSGVHAATQAKEGKGETKGNQQTKEMQMD

Target ORF information:

RefSeq Version	XM_003962662.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes heat shock 70 kDa protein 4L (LOC101075949), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003962662.3

ORF Insert Sequence:

ATGTCAGTGG TCGGGATTGA TGTGGGTTTC CAAAGCTGTT ACATCGCTGT GGCCAGGAGC 
GGCGGCATTG AAACCATTGC CAATGAATAC AGTGACAGAT GCACACCAGC TTGTGTGTCC 
TTGGCAACTA AAAACCGTAT AATTGGAAAC GCTGCCAAAA GTCAGATCAT AACAAACTTC 
AAAAATACAG TCCATGGCTT TAAAAAGTTC CACGGCAGGA CGTTCGATGA CCCATTCGTC 
CAAGCAGAAA AGCCCAAACT GCCTTACAGC TTGCATAAAC TGGCCAATGG GAGCACTGGA 
ATTCAGGTGC GATATTTGGA CGACGACAAA GTGTTCACAG TTGAGCAGAT CACGGGGATG 
CTGCTCAGCA AGCTGAAAGA GACGTCTGAG GGGGCTCTGA AGAAGCCCGT GGTGGACTGC 
GTCATCTCCG TCCCAAGTTT TTTCACAGAC GCTGAAAGAC GTTCGGTGTT TGACGCTTCT 
CAAATTGCAG GGCTGAACTG TCTTCGGCTA ATTAACGACA CTACTGCAGT TGCCTTGGCC 
TACGGCATCT ACAAACAGGA CCTTCCTGCT CCAGAAGAGA GGCCAAGAAA CGTTGTGTTT 
GTGGACATGG GACATTCATC ATTCCAGGTC TCCATCGCTG CCTTCAACAA AGGCAAACTC 
AAGGTCCTCG CCACTGCGTT CGACCCATAC CTCGGTGGGC GCAACCTCGA CGAGGTGTTG 
GTCGATTACT TCTGCGAGGA GTTCAAGGGC AAGTACAAAC TCAATGTAAG GGAGAACCCG 
AGGGCTCTGC TGCGGCTGCA CCAGGAGTGC GAGAAGCTGA AGAAGCTGAT GAGCGCCAAC 
TCCTCCAACC TGCCTCTGAA CATCGAGTGC TTCATGAATG ACATCGACGT GTCCAGCAGG 
ATGAACAGAG GCCAATTTGA AGAAATGTCT TCTCAGTATC TGATGAGAGT GGAGGCGCCT 
TTAAGAGCCG TCCTCGAACA GTCGAAGCTA AGCCGAGATG ACGTCTACGC CGTGGAGATA 
GTTGGAGGAG CGACAAGAAT CCCGGCGATC AAAGAGCGAA TCAGCCGTTT CTTCTGCAAA 
GACATCAGCA CAACCCTCAA CGCAGATGAA GCCGTCGCTC GAGGCTGCGC ACTGCAGTGC 
GCCATTTTGT CGCCAGCGTT CAAGGTCCGC GAGTTCTCCA TCACCGACGT CGTTCCCTTT 
CCTATTACTC TCCGCTGGAA ATCTACAACA GAGGATGGAG TGGGAGAGTG TGAGGTGTTC 
TCGAAGAATC ACGCTGCTCC ATTTTCCAAA GTCATCACCT TTCACAAGAA GGAACCATTT 
GACCTTGAAG CCTTTTACAG TAATTCTCAA GAGCTTCCAT ACCCAGACCA CACCATAGGA 
TGCTTCTCTG TCCAGAACGT TTCTCCCCAG CCAGACGGAG ACAGCTCCAA AGTGAAGGTC 
AAGGTGCGGG TGAACATCCA TGGCATCTTC AGCGTGTCCA GCGCCTCTCT GATAGAGAAG 
CAGAAAGGAG AAGTGGAGGA CATGCAGATT GACTCTGAGC CACTGGTGCA GAATGAGGGG 
AGGGTGGAGG AGCAGGCTAA AATGCAGGTG GACCAGCAAG GTCAAGGTCA GGGGGAGGAA 
CAGGCCGATG AAAACAGCCC CAGCAATAAA GAGGGGCCAT CTGTGGAAAA ACAGGACCCA 
GCAGCAGGGG GGAGCAAGCC CAAGGTCAAG GTGAAGAGCA TTGATCTGCC GGTTGTGATG 
AACAGCATTA GACAGCTGGA CACATCTGTC CTAAATGACT TTGTGGAGTA TGAGCGTCAG 
ATGATTATTC AGGACAAACT AGTGAAGGAG CAGAATGATG CTAAAAATGC TGTTGAGGAG 
TACGTGTACG ATCTGCGGGA CAAACTCGGA GGCGTCTACG AAAAGTACAT CACCGAGAAT 
GACAGTAAAC AGTTGACACT GATGCTGGAG GAAACCGAGA ACTGGCTGTA TGAGGATGGA 
GAGGACCAGC CCAAACGAGT GTATGAGGAG AAGTTGGAGG CCCTCAAGAG GTTGGGTCAG 
CCCATTCAGG ACCGGCACAG AGAGCACGAG GACAGGCCGA GGGCTTTTGA AGAGCTGGGA 
AAGAAGCTGC AAGTCTACAT GAAGTTTTTG GATTCCTTTA AGCAGAAGGA TGAGCGATTC 
ATGCATTTAA ATGTGGAAGA AATGGGCACA GTGGAGAAAT GTGTGAGTGA CAGCATGGGC 
TGGATGAACA GCAAAATGAA TGCACAGAGC AAGCTGAGCA TTACTCAAGA CCCCGCTGTT 
AAAGTAGCAG ACATCATCGC TAAAATACAG GAGCTAGAGG ACGCGTGTCA TCCAGTGATC 
AACAGACCCA AGCCCAGTGC GGAGGAGACC TCTGAGGTGA ATGAGCAAAG CAGTGGAGTG 
CACGCAGCCA CCCAGGCTAA AGAAGGAAAG GGAGAAACTA AGGGAAACCA GCAGACCAAA 
GAGATGCAGA TGGACTGA