ap4e1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	ap4e1
Entrez Gene ID	101074794
Full Name	adaptor related protein complex 4 epsilon 1 subunit
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003967747.2	XP_003967796.1	AP-4 complex subunit epsilon-1
XM_003967747.3	XP_003967796.2	AP-4 complex subunit epsilon-1

Pathways from BioSystems
Gene Ontology

The following ap4e1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ap4e1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo57401	XM_003967747.3 Latest version!	Takifugu rubripes adaptor related protein complex 4 subunit epsilon 1 (ap4e1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo06697	XM_003967747.2 Latest version!	Takifugu rubripes adaptor-related protein complex 4, epsilon 1 subunit (ap4e1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ap4e1 ( XM_003967747.3 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003967796.2 Takifugu rubripes adaptor related protein complex 4 subunit epsilon 1 (ap4e1), mRNA.

Price & Availability↑

CloneID	OTo57401
Clone ID Related Accession (Same CDS sequence)	XM_003967747.3
Accession Version	XM_003967747.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	AP-4 complex subunit epsilon-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003967747.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGATG TGGTGGAGAA GACCCTGACA GCTCTGCCGA GCCTGCTGTC TTTGGACTCT 
CAGCCCGGTT CGGCTAAACT GTCATCGTCC TCGAAACTTG GAAGCCTTAT TAGAGGAATC 
ACCGAGTTAA CTTCAAAACA TGAGGAGCAA AAGCTTATAG AGGCCGAGCT TACATCCATC 
AAAGAACAAG TGTCTTCCCC GCACAACTCA ATGAGGCAGA TGAAGGAGCT CATGGTTAGA 
GCAATCTACT GTGAAATGCT GGGCTACGAC GCCTCATTTG CCTATATTCA CGCTATCAAA 
CTGGCTCAAC AGGGCACTGC TTTGGAAAAA AGAGTGGGTT ATCTTGCTGT GTCTTTGTTT 
CTGAATGAAA GTCACGAGCT GCTGCTTCTC CTTGTGAACA CAGTATTAAA GGATCTTCAG 
AGCACCAACC TTATTGAAGT GTGCATGGCT CTCACTGTTG TGAGTCAGAT TTTCCCCAAA 
GATATGATTC CTGCCATCCT TCCTCTGGTC GAGGAGAAAC TTAATAACCC AAAAGAAATT 
ATTCGAAGAA AAGCAGTATT GGCACTGTAT AAATTCTATC TAATCGCTCC AACTCAAGTT 
CAGCACATCC CCAACAAGTT TCGCAAAGCT CTGTGTGACA AGGACCCCGG TGTGATGACG 
GCATCACTCC ACATCTACCT TCAAATGATC CAGGAGAATC CAGAAAGTTA CAAAGACCTG 
ACCTCAAGTA TTGTCACTAT ACTGAAACAA GTGGTGGGAG GAAAGTTACC AATTGATTTT 
AACTATCACA GTGTCCCTGC ACCGTGGCTG CAAATTCAGC TCCTTAGAAT ACTCGCTCTA 
CTTGGGAAAA ATGACCAGAG TACAAGTGAA ATAATGTATG AGGTCCTTGA CGAGTCTTTG 
CAAAGGGCAG AGATGAATCA TAACATCACC TATGCAATAT TATACGAGTG TGTAAAATGC 
ATTTACACAG TTTACCCCAA GTCGGAGCTT CTGGAAAAAG CTGCAAAGTG CATCGGCAAC 
TTTATTTTGT CACCAAAGAT AAACCTCAAA TATCTTGGCT TGAAGGCTCT CACCTACGTG 
GTTCAGCAGG ATCCTAAACT GGCCCTACAG CATCAGATGA CCATCATAGA ATGTCTCGAC 
CACACTGACC TCATCATCAA GCGTGAGACA CTGGAGCTGC TCTTTCGAAT CACAAACTCC 
CAGAATGTCA CAGTCATTGT TGAGAAGATG CTGGAGTTCC TACGCACCAG TAAAGACGAC 
TACGCCACCA CTGACTTAGT GGGGAAGGTG TCAGAATTGG CAGAAAAATA TGCCCCTGAT 
AATGAATGGT TCATTGAGAC CATGAACACG GTGTTTTCTT TGGGTGGAGA CATGATGCAC 
CCCGATCTCC CAAACAGCTT CGTGAAACTC CTCTCTGAGG GATTTGAAAG TGTTGAGGAG 
GACAGGAAGA TGAAGCTGTT CGCAGTTGAT TCTTACATTT CTCTGCTACA AGGAGAGCCT 
GAAAAACTGC CACAGCGCTT CCTCCAGGTC ATTAGCTGGG TCCTGGGTGA ATACTCCTGG 
CTGAAAGAGG ACTTGGAACC AGGCACAGTC CTGAAGCTGA TGGCCAATCT GCTGGACTTG 
AAGAGCACCA GCAGTGAAAC CAAGACCTGG GTCCTGTTGG CAATGGCTAA GCAGTGTGAG 
GGCGGCAGCA CGGACGTCTC TGTGACCCGG AAGGTTTGTG AAACGTACAG CAGCTCCTTG 
GACACTGTGC TGAGGCAGAG AGCTCAGGAA CTGCAGTACC TCAGCCAGGA CTCTGAACTA 
CAGGCCCGGG TGCTGCCTCG GGATGCCTCC AGGGAACCCT TGGAGGTGGA TTCATCGCTG 
TCGTTCCTGG ATGCATTTGT CTCGGAAGCG TTGGCTGCCG GTGCTGCTCC GTACAAGCCC 
CCCCATCAGC GGCAGGAGGA ACTGGCTGAG GGCAAAGCGT TGAGTCTGGA GCCGTACGGC 
CTGTCTCTGC CCATCAGCAT GTCCTCCTGC AGCATCACTG ACAGACAGTC GCCCACGCTG 
TTGTCCATCA GCTCTGGTCT CTCAGGCAAC AGCGTAGACC GCTCACACAA AGGAGGTTCT 
ACATCTCTGA ACTTGGATGG TGTGAGGAGG GTGTGGGGGA AGGATGGCTA CCTGGTAAAG 
AGAGAACCTG TGGAGGAGGT GGGCCAGGTT GAGGTTCCTT CCCTTCAGCT CTCCCCCAGC 
CTGGAGGCAG AGGCAGCAGA CTCCCCCAGT CAGACCCCCA CCTCTGAACC CACCCCCGAT 
CTGGAGCCAG GGAAACAGCA ACTAGCCTCC TCGCTGTTTG TCGGCCTGGC TTCCCCCAGC 
TCCGTGTCTC TGATGGGAAA GACTGAGGAG ACCCCCCACC GTTTCAGAAG AAAAGCCAAA 
GTCTCAGACC CGTCCCAGGA GGGAACGTCA AGGTCTACGT TCGCTTCTTC CTGCTCGGTG 
GACAACTTCC TGTGTGAGAA CCTTCTGGAC CCCGAGTCGG CTGTTTCCTC CCCATCGCAG 
GCTTCCCAGC TCTCTCTGAG CCTCGGCACT ACAAACGGCA TCTGTGATGT GGAGTTCGCT 
GGCAATAATG TGGAGGCAGA AAAGCCCGCT CCCGCTGTAG ATAACCTCGC TAACGAAGGG 
ACTGAACTCG CTCGTCGTGA GCGGCAGCAA ACGCCCAAAG ACTTTTGTCT CGGTTCTCAT 
GTTCCTGCAG AGCTCAGGGG CCTCTCCCGC TCTGATATCG CCCCCCTGAG CTCCAACCAA 
AGCCTGGATC TCTCAGCCTG TCACGTGCAG AAAGAGGACG CCTTAGTTCT GCTTCTGTTC 
TTCTGTAACT GCTCTTCCTC GGACATTCAA CATATGCGCC TTGAACTCCA CTCGGATGAA 
CTGGAGGTGT CCTGGTGTGG GGGCGTGATG CAGGAGGTGA GGAGTCACGC CGTAGCGGTG 
TGTCAGTCTT CGCTGATCGT GAAGAGGCCC TCAGTCCACG TGGAAGTGCG CGGGGTCGTT 
TCTTACTCTC TGCCTGCCGG GACACCTCTG TCACTGCAGT TCTCGTACAA ACTTCCTCTC 
AGCAGCTTCA TCAGACCTCT GGCCATGTCC ACCGAGGAGT ACGGCAAAAT GTGGCTGGAC 
TTCTCACACG ACACCAGGCA GAACCTGAAG CTGATAGGCG ACGGCCTGGT GGACACTCTG 
AACGTGCTGA CGAACAAACT TCAGCTCCAC GTGGTAGAAA TCATAGGTGT GGAGGGGATT 
GTAGCCTGCC AGCTGCTCCA CAATCAGCCG TGTCTCCTGC ACTGTCGCCT GCACGCAGGA 
GCTCTGGCGG TGTGGCTGCG CTCTCCGGTG CCCGAGCTGC CCGACTCCCT GATCTATCAC 
TGTCAGAGAG TTTTAAACGA ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003967796.2
CDS	196..3582
Translation	MSDVVEKTLTALPSLLSLDSQPGSAKLSSSSKLGSLIRGITELTSKHEEQKLIEAELTSIKEQVSSPHNSMRQMKELMVRAIYCEMLGYDASFAYIHAIKLAQQGTALEKRVGYLAVSLFLNESHELLLLLVNTVLKDLQSTNLIEVCMALTVVSQIFPKDMIPAILPLVEEKLNNPKEIIRRKAVLALYKFYLIAPTQVQHIPNKFRKALCDKDPGVMTASLHIYLQMIQENPESYKDLTSSIVTILKQVVGGKLPIDFNYHSVPAPWLQIQLLRILALLGKNDQSTSEIMYEVLDESLQRAEMNHNITYAILYECVKCIYTVYPKSELLEKAAKCIGNFILSPKINLKYLGLKALTYVVQQDPKLALQHQMTIIECLDHTDLIIKRETLELLFRITNSQNVTVIVEKMLEFLRTSKDDYATTDLVGKVSELAEKYAPDNEWFIETMNTVFSLGGDMMHPDLPNSFVKLLSEGFESVEEDRKMKLFAVDSYISLLQGEPEKLPQRFLQVISWVLGEYSWLKEDLEPGTVLKLMANLLDLKSTSSETKTWVLLAMAKQCEGGSTDVSVTRKVCETYSSSLDTVLRQRAQELQYLSQDSELQARVLPRDASREPLEVDSSLSFLDAFVSEALAAGAAPYKPPHQRQEELAEGKALSLEPYGLSLPISMSSCSITDRQSPTLLSISSGLSGNSVDRSHKGGSTSLNLDGVRRVWGKDGYLVKREPVEEVGQVEVPSLQLSPSLEAEAADSPSQTPTSEPTPDLEPGKQQLASSLFVGLASPSSVSLMGKTEETPHRFRRKAKVSDPSQEGTSRSTFASSCSVDNFLCENLLDPESAVSSPSQASQLSLSLGTTNGICDVEFAGNNVEAEKPAPAVDNLANEGTELARRERQQTPKDFCLGSHVPAELRGLSRSDIAPLSSNQSLDLSACHVQKEDALVLLLFFCNCSSSDIQHMRLELHSDELEVSWCGGVMQEVRSHAVAVCQSSLIVKRPSVHVEVRGVVSYSLPAGTPLSLQFSYKLPLSSFIRPLAMSTEEYGKMWLDFSHDTRQNLKLIGDGLVDTLNVLTNKLQLHVVEIIGVEGIVACQLLHNQPCLLHCRLHAGALAVWLRSPVPELPDSLIYHCQRVLNEH

Target ORF information:

RefSeq Version	XM_003967747.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes adaptor related protein complex 4 subunit epsilon 1 (ap4e1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003967747.3

ORF Insert Sequence:

ATGAGCGATG TGGTGGAGAA GACCCTGACA GCTCTGCCGA GCCTGCTGTC TTTGGACTCT 
CAGCCCGGTT CGGCTAAACT GTCATCGTCC TCGAAACTTG GAAGCCTTAT TAGAGGAATC 
ACCGAGTTAA CTTCAAAACA TGAGGAGCAA AAGCTTATAG AGGCCGAGCT TACATCCATC 
AAAGAACAAG TGTCTTCCCC GCACAACTCA ATGAGGCAGA TGAAGGAGCT CATGGTTAGA 
GCAATCTACT GTGAAATGCT GGGCTACGAC GCCTCATTTG CCTATATTCA CGCTATCAAA 
CTGGCTCAAC AGGGCACTGC TTTGGAAAAA AGAGTGGGTT ATCTTGCTGT GTCTTTGTTT 
CTGAATGAAA GTCACGAGCT GCTGCTTCTC CTTGTGAACA CAGTATTAAA GGATCTTCAG 
AGCACCAACC TTATTGAAGT GTGCATGGCT CTCACTGTTG TGAGTCAGAT TTTCCCCAAA 
GATATGATTC CTGCCATCCT TCCTCTGGTC GAGGAGAAAC TTAATAACCC AAAAGAAATT 
ATTCGAAGAA AAGCAGTATT GGCACTGTAT AAATTCTATC TAATCGCTCC AACTCAAGTT 
CAGCACATCC CCAACAAGTT TCGCAAAGCT CTGTGTGACA AGGACCCCGG TGTGATGACG 
GCATCACTCC ACATCTACCT TCAAATGATC CAGGAGAATC CAGAAAGTTA CAAAGACCTG 
ACCTCAAGTA TTGTCACTAT ACTGAAACAA GTGGTGGGAG GAAAGTTACC AATTGATTTT 
AACTATCACA GTGTCCCTGC ACCGTGGCTG CAAATTCAGC TCCTTAGAAT ACTCGCTCTA 
CTTGGGAAAA ATGACCAGAG TACAAGTGAA ATAATGTATG AGGTCCTTGA CGAGTCTTTG 
CAAAGGGCAG AGATGAATCA TAACATCACC TATGCAATAT TATACGAGTG TGTAAAATGC 
ATTTACACAG TTTACCCCAA GTCGGAGCTT CTGGAAAAAG CTGCAAAGTG CATCGGCAAC 
TTTATTTTGT CACCAAAGAT AAACCTCAAA TATCTTGGCT TGAAGGCTCT CACCTACGTG 
GTTCAGCAGG ATCCTAAACT GGCCCTACAG CATCAGATGA CCATCATAGA ATGTCTCGAC 
CACACTGACC TCATCATCAA GCGTGAGACA CTGGAGCTGC TCTTTCGAAT CACAAACTCC 
CAGAATGTCA CAGTCATTGT TGAGAAGATG CTGGAGTTCC TACGCACCAG TAAAGACGAC 
TACGCCACCA CTGACTTAGT GGGGAAGGTG TCAGAATTGG CAGAAAAATA TGCCCCTGAT 
AATGAATGGT TCATTGAGAC CATGAACACG GTGTTTTCTT TGGGTGGAGA CATGATGCAC 
CCCGATCTCC CAAACAGCTT CGTGAAACTC CTCTCTGAGG GATTTGAAAG TGTTGAGGAG 
GACAGGAAGA TGAAGCTGTT CGCAGTTGAT TCTTACATTT CTCTGCTACA AGGAGAGCCT 
GAAAAACTGC CACAGCGCTT CCTCCAGGTC ATTAGCTGGG TCCTGGGTGA ATACTCCTGG 
CTGAAAGAGG ACTTGGAACC AGGCACAGTC CTGAAGCTGA TGGCCAATCT GCTGGACTTG 
AAGAGCACCA GCAGTGAAAC CAAGACCTGG GTCCTGTTGG CAATGGCTAA GCAGTGTGAG 
GGCGGCAGCA CGGACGTCTC TGTGACCCGG AAGGTTTGTG AAACGTACAG CAGCTCCTTG 
GACACTGTGC TGAGGCAGAG AGCTCAGGAA CTGCAGTACC TCAGCCAGGA CTCTGAACTA 
CAGGCCCGGG TGCTGCCTCG GGATGCCTCC AGGGAACCCT TGGAGGTGGA TTCATCGCTG 
TCGTTCCTGG ATGCATTTGT CTCGGAAGCG TTGGCTGCCG GTGCTGCTCC GTACAAGCCC 
CCCCATCAGC GGCAGGAGGA ACTGGCTGAG GGCAAAGCGT TGAGTCTGGA GCCGTACGGC 
CTGTCTCTGC CCATCAGCAT GTCCTCCTGC AGCATCACTG ACAGACAGTC GCCCACGCTG 
TTGTCCATCA GCTCTGGTCT CTCAGGCAAC AGCGTAGACC GCTCACACAA AGGAGGTTCT 
ACATCTCTGA ACTTGGATGG TGTGAGGAGG GTGTGGGGGA AGGATGGCTA CCTGGTAAAG 
AGAGAACCTG TGGAGGAGGT GGGCCAGGTT GAGGTTCCTT CCCTTCAGCT CTCCCCCAGC 
CTGGAGGCAG AGGCAGCAGA CTCCCCCAGT CAGACCCCCA CCTCTGAACC CACCCCCGAT 
CTGGAGCCAG GGAAACAGCA ACTAGCCTCC TCGCTGTTTG TCGGCCTGGC TTCCCCCAGC 
TCCGTGTCTC TGATGGGAAA GACTGAGGAG ACCCCCCACC GTTTCAGAAG AAAAGCCAAA 
GTCTCAGACC CGTCCCAGGA GGGAACGTCA AGGTCTACGT TCGCTTCTTC CTGCTCGGTG 
GACAACTTCC TGTGTGAGAA CCTTCTGGAC CCCGAGTCGG CTGTTTCCTC CCCATCGCAG 
GCTTCCCAGC TCTCTCTGAG CCTCGGCACT ACAAACGGCA TCTGTGATGT GGAGTTCGCT 
GGCAATAATG TGGAGGCAGA AAAGCCCGCT CCCGCTGTAG ATAACCTCGC TAACGAAGGG 
ACTGAACTCG CTCGTCGTGA GCGGCAGCAA ACGCCCAAAG ACTTTTGTCT CGGTTCTCAT 
GTTCCTGCAG AGCTCAGGGG CCTCTCCCGC TCTGATATCG CCCCCCTGAG CTCCAACCAA 
AGCCTGGATC TCTCAGCCTG TCACGTGCAG AAAGAGGACG CCTTAGTTCT GCTTCTGTTC 
TTCTGTAACT GCTCTTCCTC GGACATTCAA CATATGCGCC TTGAACTCCA CTCGGATGAA 
CTGGAGGTGT CCTGGTGTGG GGGCGTGATG CAGGAGGTGA GGAGTCACGC CGTAGCGGTG 
TGTCAGTCTT CGCTGATCGT GAAGAGGCCC TCAGTCCACG TGGAAGTGCG CGGGGTCGTT 
TCTTACTCTC TGCCTGCCGG GACACCTCTG TCACTGCAGT TCTCGTACAA ACTTCCTCTC 
AGCAGCTTCA TCAGACCTCT GGCCATGTCC ACCGAGGAGT ACGGCAAAAT GTGGCTGGAC 
TTCTCACACG ACACCAGGCA GAACCTGAAG CTGATAGGCG ACGGCCTGGT GGACACTCTG 
AACGTGCTGA CGAACAAACT TCAGCTCCAC GTGGTAGAAA TCATAGGTGT GGAGGGGATT 
GTAGCCTGCC AGCTGCTCCA CAATCAGCCG TGTCTCCTGC ACTGTCGCCT GCACGCAGGA 
GCTCTGGCGG TGTGGCTGCG CTCTCCGGTG CCCGAGCTGC CCGACTCCCT GATCTATCAC 
TGTCAGAGAG TTTTAAACGA ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ap4e1 ( XM_003967747.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003967796.1 Takifugu rubripes adaptor-related protein complex 4, epsilon 1 subunit (ap4e1), mRNA.

Price & Availability↑

CloneID	OTo06697
Clone ID Related Accession (Same CDS sequence)	XM_003967747.2
Accession Version	XM_003967747.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	AP-4 complex subunit epsilon-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071017.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003967747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGATG TGGTGGAGAA GACCCTGACA GCTCTGCCGA GCCTGCTGTC TTTGGACTCT 
CAGCCCGGTT CGGCTAAACT GTCATCGTCC TCGAAACTTG GAAGCCTTAT TAGAGGAATC 
ACCGAGTTAA CTTCAAAACA TGAGGAGCAA AAGCTTATAG AGGCCGAGCT TACATCCATC 
AAAGAACAAG TGTCTTCCCC GCACAACTCA ATGAGGCAGA TGAAGGAGCT CATGGTTAGA 
GCAATCTACT GTGAAATGCT GGGCTACGAC GCCTCATTTG CCTATATTCA CGCTATCAAA 
CTGGCTCAAC AGGGCACTGC TTTGGAAAAA AGAGTGGGTT ATCTTGCTGT GTCTTTGTTT 
CTGAATGAAA GTCACGAGCT GCTGCTTCTC CTTGTGAACA CAGTATTAAA GGATCTTCAG 
AGCACCAACC TTATTGAAGT GTGCATGGCT CTCACTGTTG TGAGTCAGAT TTTCCCCAAA 
GATATGATTC CTGCCATCCT TCCTCTGGTC GAGGAGAAAC TTAATAACCC AAAAGAAATT 
ATTCGAAGAA AAGCAGTATT GGCACTGTAT AAATTCTATC TAATCGCTCC AACTCAAGTT 
CAGCACATCC CCAACAAGTT TCGCAAAGCT CTGTGTGACA AGGACCCCGG TGTGATGACG 
GCATCACTCC ACATCTACCT TCAAATGATC CAGGAGAATC CAGAAAGTTA CAAAGACCTG 
ACCTCAAGTA TTGTCACTAT ACTGAAACAA GTGGTGGGAG GAAAGTTACC AATTGATTTT 
AACTATCACA GTGTCCCTGC ACCGTGGCTG CAAATTCAGC TCCTTAGAAT ACTCGCTCTA 
CTTGGGAAAA ATGACCAGAG TACAAGTGAA ATAATGTATG AGGTCCTTGA CGAGTCTTTG 
CAAAGGGCAG AGATGAATCA TAACATCACC TATGCAATAT TATACGAGTG TGTAAAATGC 
ATTTACACAG TTTACCCCAA GTCGGAGCTT CTGGAAAAAG CTGCAAAGTG CATCGGCAAC 
TTTATTTTGT CACCAAAGAT AAACCTCAAA TATCTTGGCT TGAAGGCTCT CACCTACGTG 
GTTCAGCAGG ATCCTAAACT GGCCCTACAG CATCAGATGA CCATCATAGA ATGTCTCGAC 
CACACTGACC TCATCATCAA GCGTGAGACA CTGGAGCTGC TCTTTCGAAT CACAAACTCC 
CAGAATGTCA CAGTCATTGT TGAGAAGATG CTGGAGTTCC TACGCACCAG TAAAGACGAC 
TACGCCACCA CTGACTTAGT GGGGAAGGTG TCAGAATTGG CAGAAAAATA TGCCCCTGAT 
AATGAATGGT TCATTGAGAC CATGAACACG GTGTTTTCTT TGGGTGGAGA CATGATGCAC 
CCTGATCTCC CAAACAGCTT CGTGAAACTC CTCTCTGAGG GATTTGAAAG TGTTGAGGAG 
GACAGGAAGA TGAAGCTGTT CGCAGTTGAT TCTTACATTT CTCTGCTACA AGGAGAGCCT 
GAAAAACTGC CACAGCGCTT CCTCCAGGTC ATTAGCTGGG TCCTGGGTGA ATACTCCTGG 
CTGAAAGAGG ACTTGGAACC AGACACAGTC CTGAAGCTGA TGGCCAGTCT GCTGGACTTG 
AAGAGCACCA GCAGTGAAAC CAAGACCTGG GTCCTGTTGG CAATGGCTAA GCAGTGTGAG 
GGCGGCAGCA CGGACGTCTC TGTGACCCGG AAGGTTTGTG AAACGTACAG CAGCTCCTTG 
GACACTGTGC TGAGGCAGAG AGCTCAGGAA CTGCAGTACC TCAGCCAGGA CTCTGAACTA 
CAGGCCCGGG TGCTGCCTCG GGATGCCTCC AGGGAACCCT TGGAGGTGGA TTCATCGCTG 
TCGTTCCTGG ATGCATTTGT CTCGGAAGCG TTGGCTGCCG GTGCTGCTCC GTACAAGCCC 
CCCCATCAGC GGCAGGAGGA ACTGGCTGAG GGCAAAGCGT TGAGTCTGGA GCCGTACGGC 
CTGTCTCTGC CCATCAGCAT GTCCTCCTGC AGCATCACTG ACAGACAGTC GCCCACGCTG 
TTGTCCATCA GCTCTGGTCT CTCAGGCAAC AGCGTAGACC GCTCACACAA AGGAGGTTCT 
ACATCTCTGA ACTTGGATGG TGTGAGGAGG GTGTGGGGGA AAGATGGCTA CCTGGTAAAG 
AGAGAACCTG TGGAGGAGGT GGGCCAGGTT GAGGTTCCTT CCCTTCAGCT CTCCCCCAGC 
CTGGAGGCAG AGGCAGCAGA CTCCCCCAGT CAGACCCCCA CCTCTGAACC CACCCCCGAT 
CTGGAGCCAG GGAAACAGCA ACTAGCCTCC TCGCTGTTTG TCGGCCTGGC TTCCCACAGC 
TCCGTGTCTC TGATGGGAAA GACTGAGGAG ACCCCCCACC GTTTCAGAAG AAAAGCCAAA 
GTCTCAGACC CGTCCCAGGA GGGAACGCCA AGGTCTACGT TCGCTTCTTC CTGCTCGGTG 
GACAACTTCC TGTGTGAGAA CCTTCTGGAC CCCGAGTCGG CTGTTTCCTC CCCATCGCAG 
GCTTCCCAGC TCTCTCTGAG CCTCGGCACT ACAAACGGCA TCTGTGATGT GGAGTTCGCT 
GGCAATAATG TGGAGGCAGA AAAGCCCGCT CCCGCTGTAG ATAACCTCGC TAACGAAGGG 
ACTGAACTCG CTCGTCGTGA GCGGCAGCAA ACGCCCAAAG ACTTTTGTCT CGGTTCTCAT 
GTTCCTGCAG AGCTCAGGGG CCTCTCCCGC TCTGATATCG CCCCCCTGAG CTCCAACCAA 
AGCCTGGATG TCTCAGCCTG TCACGTGCAG AAAGAGGACG CCTTAGTTCT GCTTCTTTTC 
TTCTGTAACT GCTCTTCCTC GGACATTCAA CATATGCGCC TTGAACTCCA CTCGGATGAA 
CTGGAGGTGT CCTGGTGTGG GGGCGTGATG CAGGAGGTGA GGAGTCACGC CGTAGCGGTG 
TGTCAGTCTT CGCTGATCGT GAAGAGGCCC TCAGTCCACG TGGAAGTGCG CGGGGTCGTT 
TCTTACTCTC TGCCTGCCGG GACACCTCTG TCACTGCAGT TCTCGTACAA ACTTCCTCTC 
AGCAGCTTCA TCAGACCTCT GGCCATGTCC ACCGAGGAGT ACGGCAAAAT GTGGCTGGAC 
TTCTCACACG ACACCAGGCA GAACCTGAAG CTGATAGGCG ACGGCCTGGT GGACACTCTG 
AACGTGCTGA CGAACAAACT TCAGCTCCAC GTGGTAGAAA TCATAGGTGT GGAGGGGATT 
GTAGCCTGCC AGCTGCTCCA CAATCAGCCG TGTCTCCTGC ACTGTCGCCT GCACGCAGGA 
GCTCTGGCGG TGTGGCTGCG CTCTCCGGTG CCCGAGCTGC CCGACTCCCT GATCTATCAC 
TGTCAGAGAG TTTTAAACGA ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003967796.1
CDS	196..3582
Translation	MSDVVEKTLTALPSLLSLDSQPGSAKLSSSSKLGSLIRGITELTSKHEEQKLIEAELTSIKEQVSSPHNSMRQMKELMVRAIYCEMLGYDASFAYIHAIKLAQQGTALEKRVGYLAVSLFLNESHELLLLLVNTVLKDLQSTNLIEVCMALTVVSQIFPKDMIPAILPLVEEKLNNPKEIIRRKAVLALYKFYLIAPTQVQHIPNKFRKALCDKDPGVMTASLHIYLQMIQENPESYKDLTSSIVTILKQVVGGKLPIDFNYHSVPAPWLQIQLLRILALLGKNDQSTSEIMYEVLDESLQRAEMNHNITYAILYECVKCIYTVYPKSELLEKAAKCIGNFILSPKINLKYLGLKALTYVVQQDPKLALQHQMTIIECLDHTDLIIKRETLELLFRITNSQNVTVIVEKMLEFLRTSKDDYATTDLVGKVSELAEKYAPDNEWFIETMNTVFSLGGDMMHPDLPNSFVKLLSEGFESVEEDRKMKLFAVDSYISLLQGEPEKLPQRFLQVISWVLGEYSWLKEDLEPDTVLKLMASLLDLKSTSSETKTWVLLAMAKQCEGGSTDVSVTRKVCETYSSSLDTVLRQRAQELQYLSQDSELQARVLPRDASREPLEVDSSLSFLDAFVSEALAAGAAPYKPPHQRQEELAEGKALSLEPYGLSLPISMSSCSITDRQSPTLLSISSGLSGNSVDRSHKGGSTSLNLDGVRRVWGKDGYLVKREPVEEVGQVEVPSLQLSPSLEAEAADSPSQTPTSEPTPDLEPGKQQLASSLFVGLASHSSVSLMGKTEETPHRFRRKAKVSDPSQEGTPRSTFASSCSVDNFLCENLLDPESAVSSPSQASQLSLSLGTTNGICDVEFAGNNVEAEKPAPAVDNLANEGTELARRERQQTPKDFCLGSHVPAELRGLSRSDIAPLSSNQSLDVSACHVQKEDALVLLLFFCNCSSSDIQHMRLELHSDELEVSWCGGVMQEVRSHAVAVCQSSLIVKRPSVHVEVRGVVSYSLPAGTPLSLQFSYKLPLSSFIRPLAMSTEEYGKMWLDFSHDTRQNLKLIGDGLVDTLNVLTNKLQLHVVEIIGVEGIVACQLLHNQPCLLHCRLHAGALAVWLRSPVPELPDSLIYHCQRVLNEH

Target ORF information:

RefSeq Version	XM_003967747.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes adaptor-related protein complex 4, epsilon 1 subunit (ap4e1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003967747.2

ORF Insert Sequence:

ATGAGCGATG TGGTGGAGAA GACCCTGACA GCTCTGCCGA GCCTGCTGTC TTTGGACTCT 
CAGCCCGGTT CGGCTAAACT GTCATCGTCC TCGAAACTTG GAAGCCTTAT TAGAGGAATC 
ACCGAGTTAA CTTCAAAACA TGAGGAGCAA AAGCTTATAG AGGCCGAGCT TACATCCATC 
AAAGAACAAG TGTCTTCCCC GCACAACTCA ATGAGGCAGA TGAAGGAGCT CATGGTTAGA 
GCAATCTACT GTGAAATGCT GGGCTACGAC GCCTCATTTG CCTATATTCA CGCTATCAAA 
CTGGCTCAAC AGGGCACTGC TTTGGAAAAA AGAGTGGGTT ATCTTGCTGT GTCTTTGTTT 
CTGAATGAAA GTCACGAGCT GCTGCTTCTC CTTGTGAACA CAGTATTAAA GGATCTTCAG 
AGCACCAACC TTATTGAAGT GTGCATGGCT CTCACTGTTG TGAGTCAGAT TTTCCCCAAA 
GATATGATTC CTGCCATCCT TCCTCTGGTC GAGGAGAAAC TTAATAACCC AAAAGAAATT 
ATTCGAAGAA AAGCAGTATT GGCACTGTAT AAATTCTATC TAATCGCTCC AACTCAAGTT 
CAGCACATCC CCAACAAGTT TCGCAAAGCT CTGTGTGACA AGGACCCCGG TGTGATGACG 
GCATCACTCC ACATCTACCT TCAAATGATC CAGGAGAATC CAGAAAGTTA CAAAGACCTG 
ACCTCAAGTA TTGTCACTAT ACTGAAACAA GTGGTGGGAG GAAAGTTACC AATTGATTTT 
AACTATCACA GTGTCCCTGC ACCGTGGCTG CAAATTCAGC TCCTTAGAAT ACTCGCTCTA 
CTTGGGAAAA ATGACCAGAG TACAAGTGAA ATAATGTATG AGGTCCTTGA CGAGTCTTTG 
CAAAGGGCAG AGATGAATCA TAACATCACC TATGCAATAT TATACGAGTG TGTAAAATGC 
ATTTACACAG TTTACCCCAA GTCGGAGCTT CTGGAAAAAG CTGCAAAGTG CATCGGCAAC 
TTTATTTTGT CACCAAAGAT AAACCTCAAA TATCTTGGCT TGAAGGCTCT CACCTACGTG 
GTTCAGCAGG ATCCTAAACT GGCCCTACAG CATCAGATGA CCATCATAGA ATGTCTCGAC 
CACACTGACC TCATCATCAA GCGTGAGACA CTGGAGCTGC TCTTTCGAAT CACAAACTCC 
CAGAATGTCA CAGTCATTGT TGAGAAGATG CTGGAGTTCC TACGCACCAG TAAAGACGAC 
TACGCCACCA CTGACTTAGT GGGGAAGGTG TCAGAATTGG CAGAAAAATA TGCCCCTGAT 
AATGAATGGT TCATTGAGAC CATGAACACG GTGTTTTCTT TGGGTGGAGA CATGATGCAC 
CCTGATCTCC CAAACAGCTT CGTGAAACTC CTCTCTGAGG GATTTGAAAG TGTTGAGGAG 
GACAGGAAGA TGAAGCTGTT CGCAGTTGAT TCTTACATTT CTCTGCTACA AGGAGAGCCT 
GAAAAACTGC CACAGCGCTT CCTCCAGGTC ATTAGCTGGG TCCTGGGTGA ATACTCCTGG 
CTGAAAGAGG ACTTGGAACC AGACACAGTC CTGAAGCTGA TGGCCAGTCT GCTGGACTTG 
AAGAGCACCA GCAGTGAAAC CAAGACCTGG GTCCTGTTGG CAATGGCTAA GCAGTGTGAG 
GGCGGCAGCA CGGACGTCTC TGTGACCCGG AAGGTTTGTG AAACGTACAG CAGCTCCTTG 
GACACTGTGC TGAGGCAGAG AGCTCAGGAA CTGCAGTACC TCAGCCAGGA CTCTGAACTA 
CAGGCCCGGG TGCTGCCTCG GGATGCCTCC AGGGAACCCT TGGAGGTGGA TTCATCGCTG 
TCGTTCCTGG ATGCATTTGT CTCGGAAGCG TTGGCTGCCG GTGCTGCTCC GTACAAGCCC 
CCCCATCAGC GGCAGGAGGA ACTGGCTGAG GGCAAAGCGT TGAGTCTGGA GCCGTACGGC 
CTGTCTCTGC CCATCAGCAT GTCCTCCTGC AGCATCACTG ACAGACAGTC GCCCACGCTG 
TTGTCCATCA GCTCTGGTCT CTCAGGCAAC AGCGTAGACC GCTCACACAA AGGAGGTTCT 
ACATCTCTGA ACTTGGATGG TGTGAGGAGG GTGTGGGGGA AAGATGGCTA CCTGGTAAAG 
AGAGAACCTG TGGAGGAGGT GGGCCAGGTT GAGGTTCCTT CCCTTCAGCT CTCCCCCAGC 
CTGGAGGCAG AGGCAGCAGA CTCCCCCAGT CAGACCCCCA CCTCTGAACC CACCCCCGAT 
CTGGAGCCAG GGAAACAGCA ACTAGCCTCC TCGCTGTTTG TCGGCCTGGC TTCCCACAGC 
TCCGTGTCTC TGATGGGAAA GACTGAGGAG ACCCCCCACC GTTTCAGAAG AAAAGCCAAA 
GTCTCAGACC CGTCCCAGGA GGGAACGCCA AGGTCTACGT TCGCTTCTTC CTGCTCGGTG 
GACAACTTCC TGTGTGAGAA CCTTCTGGAC CCCGAGTCGG CTGTTTCCTC CCCATCGCAG 
GCTTCCCAGC TCTCTCTGAG CCTCGGCACT ACAAACGGCA TCTGTGATGT GGAGTTCGCT 
GGCAATAATG TGGAGGCAGA AAAGCCCGCT CCCGCTGTAG ATAACCTCGC TAACGAAGGG 
ACTGAACTCG CTCGTCGTGA GCGGCAGCAA ACGCCCAAAG ACTTTTGTCT CGGTTCTCAT 
GTTCCTGCAG AGCTCAGGGG CCTCTCCCGC TCTGATATCG CCCCCCTGAG CTCCAACCAA 
AGCCTGGATG TCTCAGCCTG TCACGTGCAG AAAGAGGACG CCTTAGTTCT GCTTCTTTTC 
TTCTGTAACT GCTCTTCCTC GGACATTCAA CATATGCGCC TTGAACTCCA CTCGGATGAA 
CTGGAGGTGT CCTGGTGTGG GGGCGTGATG CAGGAGGTGA GGAGTCACGC CGTAGCGGTG 
TGTCAGTCTT CGCTGATCGT GAAGAGGCCC TCAGTCCACG TGGAAGTGCG CGGGGTCGTT 
TCTTACTCTC TGCCTGCCGG GACACCTCTG TCACTGCAGT TCTCGTACAA ACTTCCTCTC 
AGCAGCTTCA TCAGACCTCT GGCCATGTCC ACCGAGGAGT ACGGCAAAAT GTGGCTGGAC 
TTCTCACACG ACACCAGGCA GAACCTGAAG CTGATAGGCG ACGGCCTGGT GGACACTCTG 
AACGTGCTGA CGAACAAACT TCAGCTCCAC GTGGTAGAAA TCATAGGTGT GGAGGGGATT 
GTAGCCTGCC AGCTGCTCCA CAATCAGCCG TGTCTCCTGC ACTGTCGCCT GCACGCAGGA 
GCTCTGGCGG TGTGGCTGCG CTCTCCGGTG CCCGAGCTGC CCGACTCCCT GATCTATCAC 
TGTCAGAGAG TTTTAAACGA ACACTGA