chtf18 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	chtf18
Entrez Gene ID	101065919
Full Name	chromosome transmission fidelity factor 18
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011612786.2	XP_011611088.2	chromosome transmission fidelity protein 18 homolog
XM_011612786.1	XP_011611088.1	chromosome transmission fidelity protein 18 homolog

Pathways from BioSystems
Gene Ontology

The following chtf18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chtf18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo22174	XM_011612786.1 Latest version!	Takifugu rubripes CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) (chtf18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OTo53555	XM_011612786.2 Latest version!	Takifugu rubripes chromosome transmission fidelity factor 18 (chtf18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chtf18 ( XM_011612786.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011611088.1 Takifugu rubripes CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) (chtf18), mRNA.

Price & Availability↑

CloneID	OTo22174
Clone ID Related Accession (Same CDS sequence)	XM_011612786.1
Accession Version	XM_011612786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	chromosome transmission fidelity protein 18 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071115.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAAT ATGACGAGCT GTTCGAGATT GAAGATAAGT TCGAAGACCA GTTTGCCGAT 
GAGTTGGAAG TTTTGGCTGC GATGGAAGAA GATGAATCTT TCAAGCCGAG CAAACGTCAA 
AAGAGGGGTC AAGGAGATGA CATATCGGAC ATAGAACACT TGCTGGATGA GCCAACCACT 
CCAAAGACAA AGAGGCAAAG GCAGGAGGCA GGAGTGGCCA AACGACTTTT TGACCCTGGG 
CAAAGGAAAG CCCCACAACA GAGTTATGAC ATCACACCGC CATCATCACC AGAGCAGTAC 
GACCTCTTTG ACACTGCCAG GCCTGTCCGT TCTGTGATGG ACATCAGTGG TTTAGCTACA 
ATTCCAGAGA CTCCCAGCCG TCCTCCCACA GCGGCTCCAC CATCACAGCA CGTGCTAAAG 
CGTCCTCCTT TAGAGGGGGA CTACATCACT GTGACAGACT CGTCAGGGAA CCGTGTGTAT 
CTCAGACAAA AAGAAGATGC TGGCACGAAG GTGGCAAGTT CTAGGATAGT CCCAGACTCT 
TTTGGTGCAC TCGGGCTGTT GACAGTACCA ATTGGAGTGC TAAGGGAACA AGAAATAAAG 
AGGCGTCATG ATCAGCTGGT GGAAGAATCT GAGCGTCTTA CTGAGCTGCT GACCGGCAGT 
GTGAATGATG TGATTGTGGA GCCTGAAAGT ATAGAAGATA AAGAGAGTGA TGATCTTGAA 
GACATAGAAG GGCAAATGTC GCGGCTGTGG GTGGACAGAT TTTCTCCCCG TCACTACACT 
GAGCTCCTCA GTGACGATTT TACCAACCGA TGTTTGCTCA AGTGGTTAAA ACTTTGGGAC 
GGCGTTGTGT TTGGAAGAGA GAGGAAGTCC CACCCTGCTA GATTTGACAG ACAGGCTGCT 
GGACAGAACA CTTCTAAACC AAATCATACC AATCAGAATC CAAACCGCTT CAAGACTAAA 
GTCGAGATGA CCGAGGAGAT TCTGGAGGCT GAACTGGACC AGTACAAACG CCCTAAATTC 
AAAGTGGCGC TGTTATCAGG TCCACCAGGT TTGGGCAAGA CCACTTTGGC TCATATTATA 
GCAAAGCAAG CTGGATACAA TGTGGTGGAA ATTAATGCCA GTGATGATCG CAGCGCAGAG 
GTGTTTCAGA AACGCATCGA CACAGCAACA CAGATGAAGT CGGTTTTAGG TGTCAATGAA 
AAGCCGAACT GCCTGATTAT TGATGAAATC GATGGGGCAC CGACAGCTGC TATCAACATT 
CTGTTAGCTG CCCTGAACAG GAAAGATGGA CACAGCGGTG AAGCTACAGA GACAATGAAG 
AAGAAGAAAA AGAAAGAGTC CATCCTGCTT CGACCCATAA TCTGCATCTG TAATGACTTA 
TATGTTCCAG CTCTCAGACC GCTCAGGCAG CAGGCCTTTC TCCTGACCTT CCCCCAGACT 
CAGCCTTCCC GCCTCTCTCA GAGGCTGACA GAGATCTCCC TTCTGCATGG GATGAAGGCA 
GACACAGGCG CTCTGATGTC ACTGTGCGAG AAGACGGACA ATGACATTAG AGCCTGCATC 
AACACACTGC AGTTCCTTTA TGGTCGTGGC CTGAAGCAGG TGGACGTCAG AACCATTCAG 
AGTGTCTCTG TGGGGCAGAA GGACCAGAAC AAAGGCCTGT TTCATCTTTG GCAGGAGATC 
TTCCAGTTAC CACGAACAAA ACGGAAGCGG ATTGGGGAAG GGTTTGAGGA AGCTCCTGGT 
TCTGGAAGCG GCTCGCAGAG GTTCCATCAT GTGTTTCACT TGGCCTCATC AAGTGGAGAG 
TATGAAAAGG TTTCCCAGGG TCTGTACGAT AATTACCTGT CCATGCGTAT AAGAGACCCA 
AACCTTCAGA GTGTCTGTGA GGCCCTAGAG TGGCTCTCCT TCTCAGACCG GCTCAACCAA 
GAAATTCTGC ACTGCCAGAA CTTCTCCCTG ATGAGATACC TTCCCTTCCT GTCTGTCACA 
TTCCACTTCC TGTTTGCACA TACACACGTG CCCAGAATCA CCTATCCTCA CAGCCAGCAT 
GAGGCCTTCT CCCGGCTCAA CAGCAGCAGA AATGCTCTCT CCACAATGTT GAGTGACATC 
CCAGCATGCA TCAGGACAAG AACCAGCCAA CAAATCCTGG CCCTGGATAT GCTCCCACTG 
CTTCTGGACA TCCTCTGTCC CAAACTGCGA CCTGTGAATC CTCAGCTGTT CAGCAGCAGA 
GAGAAGGAAC AGATGCATGA GCTTGTGGAC ACCATGCTGG CCTACAACCT TTCCTACAGG 
CAGGACCGAA CACCTGAGGG GCAATACACA TACATACTGG AGCCGCGTGT TGAGGAAGTG 
GTGAGGTATC CCAGCCTGCC TCCCCGCCGT CAGCTGACCT ATCAGGCCAA ACAAACCATC 
AGCAGAGAGA TGGAGCAAGA GAAGATGAGG AGAGCCGAGC AGCTGATCCT GCAGAGAAAC 
CCCGCAGGGA CTTTGATACA GAAACAGGAG GAGAAGAAGA ACCGTGGATC CAAACTAACC 
AGCAACCATC AGCAAAGGCT GGAGAACATT GTTAAACAGA CCACAGTGGA GGCCAGGCCC 
GAGGTGGATT TCTTTGGTCG AGCCATCACC CCCAAACCAA AGAGAGAGCA GCTGTCCTCT 
GACAGAGGTG AAAAGTGTCC CAGTCTTCTG ATGGGAAAAG CTGTGGGCAA CAGTGATGTG 
TGGTTCCGCT TCAACGAAGG CATGTCGAAC GCAGTCCGGC GAAACGTGTA CATAAGAGAA 
CTGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011611088.1
CDS	150..2918
Translation	MDEYDELFEIEDKFEDQFADELEVLAAMEEDESFKPSKRQKRGQGDDISDIEHLLDEPTTPKTKRQRQEAGVAKRLFDPGQRKAPQQSYDITPPSSPEQYDLFDTARPVRSVMDISGLATIPETPSRPPTAAPPSQHVLKRPPLEGDYITVTDSSGNRVYLRQKEDAGTKVASSRIVPDSFGALGLLTVPIGVLREQEIKRRHDQLVEESERLTELLTGSVNDVIVEPESIEDKESDDLEDIEGQMSRLWVDRFSPRHYTELLSDDFTNRCLLKWLKLWDGVVFGRERKSHPARFDRQAAGQNTSKPNHTNQNPNRFKTKVEMTEEILEAELDQYKRPKFKVALLSGPPGLGKTTLAHIIAKQAGYNVVEINASDDRSAEVFQKRIDTATQMKSVLGVNEKPNCLIIDEIDGAPTAAINILLAALNRKDGHSGEATETMKKKKKKESILLRPIICICNDLYVPALRPLRQQAFLLTFPQTQPSRLSQRLTEISLLHGMKADTGALMSLCEKTDNDIRACINTLQFLYGRGLKQVDVRTIQSVSVGQKDQNKGLFHLWQEIFQLPRTKRKRIGEGFEEAPGSGSGSQRFHHVFHLASSSGEYEKVSQGLYDNYLSMRIRDPNLQSVCEALEWLSFSDRLNQEILHCQNFSLMRYLPFLSVTFHFLFAHTHVPRITYPHSQHEAFSRLNSSRNALSTMLSDIPACIRTRTSQQILALDMLPLLLDILCPKLRPVNPQLFSSREKEQMHELVDTMLAYNLSYRQDRTPEGQYTYILEPRVEEVVRYPSLPPRRQLTYQAKQTISREMEQEKMRRAEQLILQRNPAGTLIQKQEEKKNRGSKLTSNHQQRLENIVKQTTVEARPEVDFFGRAITPKPKREQLSSDRGEKCPSLLMGKAVGNSDVWFRFNEGMSNAVRRNVYIRELL

Target ORF information:

RefSeq Version	XM_011612786.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) (chtf18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011612786.1

ORF Insert Sequence:

ATGGACGAAT ATGACGAGCT GTTCGAGATT GAAGATAAGT TCGAAGACCA GTTTGCCGAT 
GAGTTGGAAG TTTTGGCTGC GATGGAAGAA GATGAATCTT TCAAGCCGAG CAAACGTCAA 
AAGAGGGGTC AAGGAGATGA CATATCGGAC ATAGAACACT TGCTGGATGA GCCAACCACT 
CCAAAGACAA AGAGGCAAAG GCAGGAGGCA GGAGTGGCCA AACGACTTTT TGACCCTGGG 
CAAAGGAAAG CCCCACAACA GAGTTATGAC ATCACACCGC CATCATCACC AGAGCAGTAC 
GACCTCTTTG ACACTGCCAG GCCTGTCCGT TCTGTGATGG ACATCAGTGG TTTAGCTACA 
ATTCCAGAGA CTCCCAGCCG TCCTCCCACA GCGGCTCCAC CATCACAGCA CGTGCTAAAG 
CGTCCTCCTT TAGAGGGGGA CTACATCACT GTGACAGACT CGTCAGGGAA CCGTGTGTAT 
CTCAGACAAA AAGAAGATGC TGGCACGAAG GTGGCAAGTT CTAGGATAGT CCCAGACTCT 
TTTGGTGCAC TCGGGCTGTT GACAGTACCA ATTGGAGTGC TAAGGGAACA AGAAATAAAG 
AGGCGTCATG ATCAGCTGGT GGAAGAATCT GAGCGTCTTA CTGAGCTGCT GACCGGCAGT 
GTGAATGATG TGATTGTGGA GCCTGAAAGT ATAGAAGATA AAGAGAGTGA TGATCTTGAA 
GACATAGAAG GGCAAATGTC GCGGCTGTGG GTGGACAGAT TTTCTCCCCG TCACTACACT 
GAGCTCCTCA GTGACGATTT TACCAACCGA TGTTTGCTCA AGTGGTTAAA ACTTTGGGAC 
GGCGTTGTGT TTGGAAGAGA GAGGAAGTCC CACCCTGCTA GATTTGACAG ACAGGCTGCT 
GGACAGAACA CTTCTAAACC AAATCATACC AATCAGAATC CAAACCGCTT CAAGACTAAA 
GTCGAGATGA CCGAGGAGAT TCTGGAGGCT GAACTGGACC AGTACAAACG CCCTAAATTC 
AAAGTGGCGC TGTTATCAGG TCCACCAGGT TTGGGCAAGA CCACTTTGGC TCATATTATA 
GCAAAGCAAG CTGGATACAA TGTGGTGGAA ATTAATGCCA GTGATGATCG CAGCGCAGAG 
GTGTTTCAGA AACGCATCGA CACAGCAACA CAGATGAAGT CGGTTTTAGG TGTCAATGAA 
AAGCCGAACT GCCTGATTAT TGATGAAATC GATGGGGCAC CGACAGCTGC TATCAACATT 
CTGTTAGCTG CCCTGAACAG GAAAGATGGA CACAGCGGTG AAGCTACAGA GACAATGAAG 
AAGAAGAAAA AGAAAGAGTC CATCCTGCTT CGACCCATAA TCTGCATCTG TAATGACTTA 
TATGTTCCAG CTCTCAGACC GCTCAGGCAG CAGGCCTTTC TCCTGACCTT CCCCCAGACT 
CAGCCTTCCC GCCTCTCTCA GAGGCTGACA GAGATCTCCC TTCTGCATGG GATGAAGGCA 
GACACAGGCG CTCTGATGTC ACTGTGCGAG AAGACGGACA ATGACATTAG AGCCTGCATC 
AACACACTGC AGTTCCTTTA TGGTCGTGGC CTGAAGCAGG TGGACGTCAG AACCATTCAG 
AGTGTCTCTG TGGGGCAGAA GGACCAGAAC AAAGGCCTGT TTCATCTTTG GCAGGAGATC 
TTCCAGTTAC CACGAACAAA ACGGAAGCGG ATTGGGGAAG GGTTTGAGGA AGCTCCTGGT 
TCTGGAAGCG GCTCGCAGAG GTTCCATCAT GTGTTTCACT TGGCCTCATC AAGTGGAGAG 
TATGAAAAGG TTTCCCAGGG TCTGTACGAT AATTACCTGT CCATGCGTAT AAGAGACCCA 
AACCTTCAGA GTGTCTGTGA GGCCCTAGAG TGGCTCTCCT TCTCAGACCG GCTCAACCAA 
GAAATTCTGC ACTGCCAGAA CTTCTCCCTG ATGAGATACC TTCCCTTCCT GTCTGTCACA 
TTCCACTTCC TGTTTGCACA TACACACGTG CCCAGAATCA CCTATCCTCA CAGCCAGCAT 
GAGGCCTTCT CCCGGCTCAA CAGCAGCAGA AATGCTCTCT CCACAATGTT GAGTGACATC 
CCAGCATGCA TCAGGACAAG AACCAGCCAA CAAATCCTGG CCCTGGATAT GCTCCCACTG 
CTTCTGGACA TCCTCTGTCC CAAACTGCGA CCTGTGAATC CTCAGCTGTT CAGCAGCAGA 
GAGAAGGAAC AGATGCATGA GCTTGTGGAC ACCATGCTGG CCTACAACCT TTCCTACAGG 
CAGGACCGAA CACCTGAGGG GCAATACACA TACATACTGG AGCCGCGTGT TGAGGAAGTG 
GTGAGGTATC CCAGCCTGCC TCCCCGCCGT CAGCTGACCT ATCAGGCCAA ACAAACCATC 
AGCAGAGAGA TGGAGCAAGA GAAGATGAGG AGAGCCGAGC AGCTGATCCT GCAGAGAAAC 
CCCGCAGGGA CTTTGATACA GAAACAGGAG GAGAAGAAGA ACCGTGGATC CAAACTAACC 
AGCAACCATC AGCAAAGGCT GGAGAACATT GTTAAACAGA CCACAGTGGA GGCCAGGCCC 
GAGGTGGATT TCTTTGGTCG AGCCATCACC CCCAAACCAA AGAGAGAGCA GCTGTCCTCT 
GACAGAGGTG AAAAGTGTCC CAGTCTTCTG ATGGGAAAAG CTGTGGGCAA CAGTGATGTG 
TGGTTCCGCT TCAACGAAGG CATGTCGAAC GCAGTCCGGC GAAACGTGTA CATAAGAGAA 
CTGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chtf18 ( XM_011612786.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011611088.2 Takifugu rubripes chromosome transmission fidelity factor 18 (chtf18), mRNA.

Price & Availability↑

CloneID	OTo53555
Clone ID Related Accession (Same CDS sequence)	XM_011612786.2
Accession Version	XM_011612786.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	chromosome transmission fidelity protein 18 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042301.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011612786.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAAT ATGACGAGCT GTTCGAGATT GAAGATAAGT TCGAAGACCA GTTTGCCGAT 
GAGTTGGAAG TTTTGGCTGC GATGGAAGAA GATGAATCTT TCAAGCCGAG CAAACGTCAA 
AAGAGGGGTC AAGGAGATGA CATATCGGAC ATAGAACACT TGCTGGATGA GCCAACCACT 
CCAAAGACAA AGAGGCAAAG GCAGGAGGCA GGAGTGGCCA AACGACTTTT TGACCCTGGG 
CAAAGGAAAG CCCCACAACA GAGTTATGAC ATCACACCGC CATCATCACC AGAGCAGTAC 
GACCTCTTTG ACACTGCCAG GCCTGTCCGT TCTGTGATGG ACATCAGTGG TTTAGCTACA 
ATTCCAGAGA CTCCCAGCCG TGCTCCCACA GCGGCTCCAC CATCACAGCA CGTGCTAAAG 
CGTCCTCCTT TAGAGGGGGA CTACATCACT GTGACAGACT CGTCAGGAAA CCGTGTGTAT 
CTCAGACAAA AAGAAGATGC TGGCACGAAG GTGGCAAGTT CTAGGATAGT CCCAGACTCT 
TTTGGTGCAC TCGGGCTGTT GACAGTACCA ATTGGAGTGC TAAGGGAACA AGAAATAAAG 
AGGCGTCATG ATCAGCTGGT GGAAGAATCT GAGCGTCTTA CTGAGCTGCT GACCGGCAGT 
GTGAATGATG TGATTGTGGA GCCTGAAAGT ATAGAAGATC TTGAAGACAT AGAAGGGCAA 
ATGTCGCGGC TGTGGGTGGA CAGATTTTCT CCCCGTCACT ACACTGAGCT CCTCAGTGAC 
GATTTTACCA ACCGATGTTT GCTCAAGTGG TTAAAACTTT GGGACGGCGT TGTGTTTGGA 
AGAGAGAGGA AGTCCCACCC TGCTAGATTT GACAGACAGG CTGCTGGACA GAACACTTCT 
AAACCAAATC ATACCAATCA GAATCCAAAC CGCTTCAAGA CTAAAGTCGA GATGACCGAG 
GAGATTCTGG AGGCTGAACT GGACCAGTAC AAACGCCCTA AATTCAAAGT GGCGCTGTTA 
TCAGGTCCAC CAGGTTTGGG CAAGACCACT TTGGCTCATA TTATAGCAAA GCAAGCTGGA 
TACAATGTGG TGGAAATTAA TGCCAGTGAT GATCGCAGCG CAGAGGTGTT TCAGAAACGC 
ATCGACACAG CAACACAGAT GAAGTCGGTT TTAGGTGTCA ATGAAAAGCC GAACTGCCTG 
ATTATTGATG AAATCGATGG GGCACCGACA GCTGCTATCA ACATTCTGTT AGCTGCCCTG 
AACAGGAAAG ATGGACACAG CGGTGAAGCT ACAGAGACAA TGAAGAAGAA GAAAAAGAAA 
GAGTCCATCC TGCTTCGACC CATAATCTGC ATCTGTAATG ACTTATATGT TCCAGCTCTC 
AGACCGCTCA GGCAGCAGGC CTTTCTCCTG ACCTTCCCCC AGACTCAGCC TTCCCGCCTC 
TCTCAGAGGC TGACAGAGAT CTCCCTTCTG CATGGGATGA AGGCAGACAC AGGCGCTCTG 
ATGTCACTGT GCGAGAAGAC GGACAATGAC ATTAGAGCCT GCATCAACAC ACTGCAGTTC 
CTTTATGGTC GTGGCCTGAA GCAGGTGGAC GTCAGAACCA TTCAGAGTGT CTCTGTGGGG 
CAGAAGGACC AGAACAAAGG CCTGTTTCAT CTTTGGCAGG AGATCTTCCA GTTACCACGA 
ACAAAACGGA AGCGGATTGG GGAAGGGTTT GAGGAAGCTC CTGGTTCTGG AAGCGGCTCG 
CAGAGGTTCC ATCATGTGTT TCACTTGGCC TCATCAAGTG GAGAGTATGA AAAGGTTTCC 
CAGGGTCTGT ACGATAATTA CCTGTCCATG CGTATAAGAG ACCCAAACCT TCAGAGTGTC 
TGTGAGGCCC TAGAGTGGCT CTCCTTCTCA GACCGGCTCA ACCAAGAAAT TCTGCACTGC 
CAGAACTTCT CCCTGATGAG ATACCTTCCC TTCCTGTCTG TCACATTCCA CTTCCTGTTT 
GCACATACAC ACGTGCCCAG AATCACCTAT CCTCACAGCC AGCATGAGGC CTTCTCCCGG 
CTCAACAGCA GCAGAAATGC TCTCTCCACA ATGTTGAGTG ACATCCCAGC ATGCATCAGG 
ACAAGAACCA GCCAACAAAT CCTGGCCCTG GATATGCTCC CACTGCTTCT GGACATCCTC 
TGTCCCAAAC TGCGACCTGT GAATCCTCAG CTGTTCAGCA GCAGAGAGAA GGAACAGATG 
CATGAGCTTG TGGACACCAT GCTGGCCTAC AACCTTTCCT ACAGGCAGGA CCGAACACCT 
GAGGGGCAAT ACACATACAT ACTGGAGCCG CGTGTTGAGG AAGTGGTGAG GTATCCCAGC 
CTGCCTCCCC GCCGTCAGCT GACCTATCAG GCCAAACAAA CCATCAGCAG AGAGATGGAG 
CAAGAGAAGA TGAGGAGAGC CGAGCAGCTG ATCCTGCAGA GAAACCCCGC AGGGACTTTG 
ATACAGAAAC AGGAGGAGAA GAAGAACCGT GGATCCAAAC TAACCAGCAA CCATCAGCAA 
AGGCTGGAGA ACATTGTTAA ACAGACCACA GTGGAGGCCA GGCCCGAGGT GGATTTCTTT 
GGTCGAGCCA TCACCCCCAA ACCACGGAGA GAGCAGCTGT CCTCTGACAG AGGTGAAAAG 
TGTCCCAGTC TTCTGATGGG AAAAGCTGTG GGCAACAGTG ATGTGTGGTT CCGCTTCAAC 
GAAGGCATGT CGAACGCAGT CCGGCGAAAC GTGTACATAA GAGAACTGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011611088.2
CDS	148..2901
Translation	MDEYDELFEIEDKFEDQFADELEVLAAMEEDESFKPSKRQKRGQGDDISDIEHLLDEPTTPKTKRQRQEAGVAKRLFDPGQRKAPQQSYDITPPSSPEQYDLFDTARPVRSVMDISGLATIPETPSRAPTAAPPSQHVLKRPPLEGDYITVTDSSGNRVYLRQKEDAGTKVASSRIVPDSFGALGLLTVPIGVLREQEIKRRHDQLVEESERLTELLTGSVNDVIVEPESIEDLEDIEGQMSRLWVDRFSPRHYTELLSDDFTNRCLLKWLKLWDGVVFGRERKSHPARFDRQAAGQNTSKPNHTNQNPNRFKTKVEMTEEILEAELDQYKRPKFKVALLSGPPGLGKTTLAHIIAKQAGYNVVEINASDDRSAEVFQKRIDTATQMKSVLGVNEKPNCLIIDEIDGAPTAAINILLAALNRKDGHSGEATETMKKKKKKESILLRPIICICNDLYVPALRPLRQQAFLLTFPQTQPSRLSQRLTEISLLHGMKADTGALMSLCEKTDNDIRACINTLQFLYGRGLKQVDVRTIQSVSVGQKDQNKGLFHLWQEIFQLPRTKRKRIGEGFEEAPGSGSGSQRFHHVFHLASSSGEYEKVSQGLYDNYLSMRIRDPNLQSVCEALEWLSFSDRLNQEILHCQNFSLMRYLPFLSVTFHFLFAHTHVPRITYPHSQHEAFSRLNSSRNALSTMLSDIPACIRTRTSQQILALDMLPLLLDILCPKLRPVNPQLFSSREKEQMHELVDTMLAYNLSYRQDRTPEGQYTYILEPRVEEVVRYPSLPPRRQLTYQAKQTISREMEQEKMRRAEQLILQRNPAGTLIQKQEEKKNRGSKLTSNHQQRLENIVKQTTVEARPEVDFFGRAITPKPRREQLSSDRGEKCPSLLMGKAVGNSDVWFRFNEGMSNAVRRNVYIRELL

Target ORF information:

RefSeq Version	XM_011612786.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes chromosome transmission fidelity factor 18 (chtf18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011612786.2

ORF Insert Sequence:

ATGGACGAAT ATGACGAGCT GTTCGAGATT GAAGATAAGT TCGAAGACCA GTTTGCCGAT 
GAGTTGGAAG TTTTGGCTGC GATGGAAGAA GATGAATCTT TCAAGCCGAG CAAACGTCAA 
AAGAGGGGTC AAGGAGATGA CATATCGGAC ATAGAACACT TGCTGGATGA GCCAACCACT 
CCAAAGACAA AGAGGCAAAG GCAGGAGGCA GGAGTGGCCA AACGACTTTT TGACCCTGGG 
CAAAGGAAAG CCCCACAACA GAGTTATGAC ATCACACCGC CATCATCACC AGAGCAGTAC 
GACCTCTTTG ACACTGCCAG GCCTGTCCGT TCTGTGATGG ACATCAGTGG TTTAGCTACA 
ATTCCAGAGA CTCCCAGCCG TGCTCCCACA GCGGCTCCAC CATCACAGCA CGTGCTAAAG 
CGTCCTCCTT TAGAGGGGGA CTACATCACT GTGACAGACT CGTCAGGAAA CCGTGTGTAT 
CTCAGACAAA AAGAAGATGC TGGCACGAAG GTGGCAAGTT CTAGGATAGT CCCAGACTCT 
TTTGGTGCAC TCGGGCTGTT GACAGTACCA ATTGGAGTGC TAAGGGAACA AGAAATAAAG 
AGGCGTCATG ATCAGCTGGT GGAAGAATCT GAGCGTCTTA CTGAGCTGCT GACCGGCAGT 
GTGAATGATG TGATTGTGGA GCCTGAAAGT ATAGAAGATC TTGAAGACAT AGAAGGGCAA 
ATGTCGCGGC TGTGGGTGGA CAGATTTTCT CCCCGTCACT ACACTGAGCT CCTCAGTGAC 
GATTTTACCA ACCGATGTTT GCTCAAGTGG TTAAAACTTT GGGACGGCGT TGTGTTTGGA 
AGAGAGAGGA AGTCCCACCC TGCTAGATTT GACAGACAGG CTGCTGGACA GAACACTTCT 
AAACCAAATC ATACCAATCA GAATCCAAAC CGCTTCAAGA CTAAAGTCGA GATGACCGAG 
GAGATTCTGG AGGCTGAACT GGACCAGTAC AAACGCCCTA AATTCAAAGT GGCGCTGTTA 
TCAGGTCCAC CAGGTTTGGG CAAGACCACT TTGGCTCATA TTATAGCAAA GCAAGCTGGA 
TACAATGTGG TGGAAATTAA TGCCAGTGAT GATCGCAGCG CAGAGGTGTT TCAGAAACGC 
ATCGACACAG CAACACAGAT GAAGTCGGTT TTAGGTGTCA ATGAAAAGCC GAACTGCCTG 
ATTATTGATG AAATCGATGG GGCACCGACA GCTGCTATCA ACATTCTGTT AGCTGCCCTG 
AACAGGAAAG ATGGACACAG CGGTGAAGCT ACAGAGACAA TGAAGAAGAA GAAAAAGAAA 
GAGTCCATCC TGCTTCGACC CATAATCTGC ATCTGTAATG ACTTATATGT TCCAGCTCTC 
AGACCGCTCA GGCAGCAGGC CTTTCTCCTG ACCTTCCCCC AGACTCAGCC TTCCCGCCTC 
TCTCAGAGGC TGACAGAGAT CTCCCTTCTG CATGGGATGA AGGCAGACAC AGGCGCTCTG 
ATGTCACTGT GCGAGAAGAC GGACAATGAC ATTAGAGCCT GCATCAACAC ACTGCAGTTC 
CTTTATGGTC GTGGCCTGAA GCAGGTGGAC GTCAGAACCA TTCAGAGTGT CTCTGTGGGG 
CAGAAGGACC AGAACAAAGG CCTGTTTCAT CTTTGGCAGG AGATCTTCCA GTTACCACGA 
ACAAAACGGA AGCGGATTGG GGAAGGGTTT GAGGAAGCTC CTGGTTCTGG AAGCGGCTCG 
CAGAGGTTCC ATCATGTGTT TCACTTGGCC TCATCAAGTG GAGAGTATGA AAAGGTTTCC 
CAGGGTCTGT ACGATAATTA CCTGTCCATG CGTATAAGAG ACCCAAACCT TCAGAGTGTC 
TGTGAGGCCC TAGAGTGGCT CTCCTTCTCA GACCGGCTCA ACCAAGAAAT TCTGCACTGC 
CAGAACTTCT CCCTGATGAG ATACCTTCCC TTCCTGTCTG TCACATTCCA CTTCCTGTTT 
GCACATACAC ACGTGCCCAG AATCACCTAT CCTCACAGCC AGCATGAGGC CTTCTCCCGG 
CTCAACAGCA GCAGAAATGC TCTCTCCACA ATGTTGAGTG ACATCCCAGC ATGCATCAGG 
ACAAGAACCA GCCAACAAAT CCTGGCCCTG GATATGCTCC CACTGCTTCT GGACATCCTC 
TGTCCCAAAC TGCGACCTGT GAATCCTCAG CTGTTCAGCA GCAGAGAGAA GGAACAGATG 
CATGAGCTTG TGGACACCAT GCTGGCCTAC AACCTTTCCT ACAGGCAGGA CCGAACACCT 
GAGGGGCAAT ACACATACAT ACTGGAGCCG CGTGTTGAGG AAGTGGTGAG GTATCCCAGC 
CTGCCTCCCC GCCGTCAGCT GACCTATCAG GCCAAACAAA CCATCAGCAG AGAGATGGAG 
CAAGAGAAGA TGAGGAGAGC CGAGCAGCTG ATCCTGCAGA GAAACCCCGC AGGGACTTTG 
ATACAGAAAC AGGAGGAGAA GAAGAACCGT GGATCCAAAC TAACCAGCAA CCATCAGCAA 
AGGCTGGAGA ACATTGTTAA ACAGACCACA GTGGAGGCCA GGCCCGAGGT GGATTTCTTT 
GGTCGAGCCA TCACCCCCAA ACCACGGAGA GAGCAGCTGT CCTCTGACAG AGGTGAAAAG 
TGTCCCAGTC TTCTGATGGG AAAAGCTGTG GGCAACAGTG ATGTGTGGTT CCGCTTCAAC 
GAAGGCATGT CGAACGCAGT CCGGCGAAAC GTGTACATAA GAGAACTGCT GTGA