map3k20 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	map3k20
Entrez Gene ID	101064896
Full Name	mitogen-activated protein kinase kinase kinase 20
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011609656.1	XP_011607958.1	mitogen-activated protein kinase kinase kinase MLT
XM_011609656.2	XP_011607958.2	mitogen-activated protein kinase kinase kinase 20

Pathways from BioSystems
Gene Ontology

The following map3k20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the map3k20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo21468	XM_011609656.1 Latest version!	Takifugu rubripes mitogen-activated protein kinase kinase kinase MLT (LOC101064896), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OTo42306	XM_011609656.2 Latest version!	Takifugu rubripes mitogen-activated protein kinase kinase kinase 20 (map3k20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

map3k20 ( XM_011609656.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011607958.1 Takifugu rubripes mitogen-activated protein kinase kinase kinase MLT (LOC101064896), mRNA.

Price & Availability↑

CloneID	OTo21468
Clone ID Related Accession (Same CDS sequence)	XM_011609656.1
Accession Version	XM_011609656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	mitogen-activated protein kinase kinase kinase MLT
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070905.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCGC CAAGCGCCTC CTTCGTGCAA ATCAAGTTTG ATGACATCCT CTTCCACGAG 
AACTGCGGCG ACGGCAGCTT CGGAAGCGTG TACCGAGCCA GATGGATCTC CCAGGACAAA 
GAGGTGGCAG TGAAAAAACT GCTCAAGATT GAAAACGAGG CTGAAATCCT CAGCGTCCTC 
AGCCACCGTA ACATCATCCA GTTTTACGGC GCCGTTGTCG AGGCTCCCAA CTATGGAATC 
GTTACTGAGT ACGCCAGCGG TGGGTCGCTG TACGACTACC TGTCCAGTGC TGAGAGCGAA 
CGGATGGACA TGGGACAGAT CATGACGTGG GCTGCAGAGA TTGCCAGAGG AATGCATTAC 
TTACATTCAG AGGCCCCCGT TAAAGTCATC CACCGCGATT TAAAATCCAG GAACGTTGTG 
GTGACTGCAG ACAAAGTCCT GAAGATCTGT GATTTTGGGG CATCTAAGTT CCTGACACAC 
ACGACACACA TGTCGCTGGT GGGCACGTTT CCGTGGATGG CTCCGGAGGT GATCCAGAGC 
TTGCCTGTGT CTGAAACGTG TGACACCTTC TCCTTCGGTG TGGTTCTTTG GGAGATGCTC 
ACCAGTGAGA TACCCTTCAA AGGCCTGGAG GGTTTACAGG TGGCCTGGCT TGTGGTGGAG 
AAAAATGAGA GGTTAACCAT CCCGAGTGGC TGTCCTGCCA GTTTTGCTGA ACTCATGAGA 
AGCTGCTGGG CATCGGAGCC AAAAGAAAGG CCAATGTTCA AGCAGATCCT CGCCACTTTG 
GAGTCCATGT CAAACGACAG CCAACTTCCT CAACAGTGTA ACTCCTTCCT GCACAACAAG 
GCTGAATGGA GGTGTGAGAT TGAAGCCACA CTTGAGAGAC TGAAAAAGCT GGAGAGAGAC 
CTGAGTACCA AAGAGCAGGA GCTGAAGGAG AGAGAGCAGC GTCTAAAGAT GTGGGAGCGG 
AAGCTCATCG AACAGTCCAG CAGCCCACTG CTGCCCACCC ATGACATCTA CACATGGACA 
GAGGAGCACG TGTATTTCTG GATGCAACAA ATATTTGGTG CAGAGGAGGG CCCCTGCGGC 
ATGCAGGTTT ACGCCAATCT GTTCAAAGAG AATCACATCA CCGGAAAGAG GTTACTGTTG 
CTCACAGAGA GCGACATGCG CGACATGGGG GTCAAGTCCA AAGGCCACGT CATGCATCTT 
AAGGGTGAAA TTGAGAAACT GACTCATGAC TATCTCAGCC TGGTCCATTT CCCTCCCCTG 
CTGAAGGAGG AACTGGAAAA GCAAGTGGAG ATGAGTAAAA CCATTAAATT GGAACTGGTG 
TTTGGGTACC ACTGGAAACC AGGAACAGGG AAAGATGACT GCAAGTGGAA AATGTACATC 
GAACTTGACG GGGACGATGT CGCTGTCACC TACATCAAAG ATGTCGTCTT TAACACCAAC 
CGTGCTGACA TGGAAACGCT GCGCATGGCA AAGCCGCCCT TTGTGATGGA GAAATGGATC 
ACTGGCATAC AGCCGAACCA GCGGGTGGAC TACACTGTCA ATTATGAGAA TGATGTCAAA 
TTGCCAAAGT CTACCAGACA CAGCTGTCCA ATGACGTGGA GTCTCATTGG TGGACAGAAC 
GAGATCAAGA CAGTGGAGCT GGTCATTGAA ACCACACCAG CAAAAATCGA CTGTAACCCA 
AAAGACAAAG ATAATTTCAA TATCGACCCC ATATGGATGT GCAAGGTTAG ACAAAGACAG 
ACGATCTCCC AGAAATCCCA AGAATCCCCC TCCGCTCTGA CGGCCTTCAC GAGCTCTGAT 
GCCCGCACCT TGCCACAGTT CCTGTCTGCC CATGAAAGAC AGGAATTTTC CTACGCTGCT 
GCTGTTCGTC GCTCACCGAA CCGCAGCCAT TTGACCCCCT GGGGTGACTC GCGCAGCTCC 
TCGCCAACCG CCAGCCTGTC AGCCAAACTT TCCCCACTTC GTCTGGGGTC AAAGGGCAGC 
AGCCCTTCCA GCACTACATC AGAGAGCACC TTAGAAAGGG ACAGAGAGCG CCCCCAGAGT 
GCCGGAGTGG TACAGGACTA TGGTAGAAAT CGCTACAATT TCTCAAATTT TGGTAGGGGG 
CAGGGCAGGG GCCAGGCATC TGCCACTTCT AGGGGCAACT ATTTTAATAA TAAAACCAGC 
AAAGCTGTAC GACAGCCAGG AAGGCCACGG TCCAACAGTT ACAGTGTCAC GCCACAGAAC 
TGCCCCACTA TGATACCTGG TATATTGTCT GTGACAGAAA ACCCCAGTGG AGAAAAAAAT 
GGGCCCAAAG CCAGTGATGG GGGGTGGATC AAAGTGGAGC GACAGAAGAG ATTAGCGCGT 
CAGGACAATA AACAAGTCAG AGGTCGGCAG AGGAGAGGGA GCAGGGGGGG GCGTGGAGCT 
GGTGGAAGAA CGGGGGCCCA CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011607958.1
CDS	182..2608
Translation	MLSPSASFVQIKFDDILFHENCGDGSFGSVYRARWISQDKEVAVKKLLKIENEAEILSVLSHRNIIQFYGAVVEAPNYGIVTEYASGGSLYDYLSSAESERMDMGQIMTWAAEIARGMHYLHSEAPVKVIHRDLKSRNVVVTADKVLKICDFGASKFLTHTTHMSLVGTFPWMAPEVIQSLPVSETCDTFSFGVVLWEMLTSEIPFKGLEGLQVAWLVVEKNERLTIPSGCPASFAELMRSCWASEPKERPMFKQILATLESMSNDSQLPQQCNSFLHNKAEWRCEIEATLERLKKLERDLSTKEQELKEREQRLKMWERKLIEQSSSPLLPTHDIYTWTEEHVYFWMQQIFGAEEGPCGMQVYANLFKENHITGKRLLLLTESDMRDMGVKSKGHVMHLKGEIEKLTHDYLSLVHFPPLLKEELEKQVEMSKTIKLELVFGYHWKPGTGKDDCKWKMYIELDGDDVAVTYIKDVVFNTNRADMETLRMAKPPFVMEKWITGIQPNQRVDYTVNYENDVKLPKSTRHSCPMTWSLIGGQNEIKTVELVIETTPAKIDCNPKDKDNFNIDPIWMCKVRQRQTISQKSQESPSALTAFTSSDARTLPQFLSAHERQEFSYAAAVRRSPNRSHLTPWGDSRSSSPTASLSAKLSPLRLGSKGSSPSSTTSESTLERDRERPQSAGVVQDYGRNRYNFSNFGRGQGRGQASATSRGNYFNNKTSKAVRQPGRPRSNSYSVTPQNCPTMIPGILSVTENPSGEKNGPKASDGGWIKVERQKRLARQDNKQVRGRQRRGSRGGRGAGGRTGAHT

Target ORF information:

RefSeq Version	XM_011609656.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes mitogen-activated protein kinase kinase kinase MLT (LOC101064896), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609656.1

ORF Insert Sequence:

ATGTTGTCGC CAAGCGCCTC CTTCGTGCAA ATCAAGTTTG ATGACATCCT CTTCCACGAG 
AACTGCGGCG ACGGCAGCTT CGGAAGCGTG TACCGAGCCA GATGGATCTC CCAGGACAAA 
GAGGTGGCAG TGAAAAAACT GCTCAAGATT GAAAACGAGG CTGAAATCCT CAGCGTCCTC 
AGCCACCGTA ACATCATCCA GTTTTACGGC GCCGTTGTCG AGGCTCCCAA CTATGGAATC 
GTTACTGAGT ACGCCAGCGG TGGGTCGCTG TACGACTACC TGTCCAGTGC TGAGAGCGAA 
CGGATGGACA TGGGACAGAT CATGACGTGG GCTGCAGAGA TTGCCAGAGG AATGCATTAC 
TTACATTCAG AGGCCCCCGT TAAAGTCATC CACCGCGATT TAAAATCCAG GAACGTTGTG 
GTGACTGCAG ACAAAGTCCT GAAGATCTGT GATTTTGGGG CATCTAAGTT CCTGACACAC 
ACGACACACA TGTCGCTGGT GGGCACGTTT CCGTGGATGG CTCCGGAGGT GATCCAGAGC 
TTGCCTGTGT CTGAAACGTG TGACACCTTC TCCTTCGGTG TGGTTCTTTG GGAGATGCTC 
ACCAGTGAGA TACCCTTCAA AGGCCTGGAG GGTTTACAGG TGGCCTGGCT TGTGGTGGAG 
AAAAATGAGA GGTTAACCAT CCCGAGTGGC TGTCCTGCCA GTTTTGCTGA ACTCATGAGA 
AGCTGCTGGG CATCGGAGCC AAAAGAAAGG CCAATGTTCA AGCAGATCCT CGCCACTTTG 
GAGTCCATGT CAAACGACAG CCAACTTCCT CAACAGTGTA ACTCCTTCCT GCACAACAAG 
GCTGAATGGA GGTGTGAGAT TGAAGCCACA CTTGAGAGAC TGAAAAAGCT GGAGAGAGAC 
CTGAGTACCA AAGAGCAGGA GCTGAAGGAG AGAGAGCAGC GTCTAAAGAT GTGGGAGCGG 
AAGCTCATCG AACAGTCCAG CAGCCCACTG CTGCCCACCC ATGACATCTA CACATGGACA 
GAGGAGCACG TGTATTTCTG GATGCAACAA ATATTTGGTG CAGAGGAGGG CCCCTGCGGC 
ATGCAGGTTT ACGCCAATCT GTTCAAAGAG AATCACATCA CCGGAAAGAG GTTACTGTTG 
CTCACAGAGA GCGACATGCG CGACATGGGG GTCAAGTCCA AAGGCCACGT CATGCATCTT 
AAGGGTGAAA TTGAGAAACT GACTCATGAC TATCTCAGCC TGGTCCATTT CCCTCCCCTG 
CTGAAGGAGG AACTGGAAAA GCAAGTGGAG ATGAGTAAAA CCATTAAATT GGAACTGGTG 
TTTGGGTACC ACTGGAAACC AGGAACAGGG AAAGATGACT GCAAGTGGAA AATGTACATC 
GAACTTGACG GGGACGATGT CGCTGTCACC TACATCAAAG ATGTCGTCTT TAACACCAAC 
CGTGCTGACA TGGAAACGCT GCGCATGGCA AAGCCGCCCT TTGTGATGGA GAAATGGATC 
ACTGGCATAC AGCCGAACCA GCGGGTGGAC TACACTGTCA ATTATGAGAA TGATGTCAAA 
TTGCCAAAGT CTACCAGACA CAGCTGTCCA ATGACGTGGA GTCTCATTGG TGGACAGAAC 
GAGATCAAGA CAGTGGAGCT GGTCATTGAA ACCACACCAG CAAAAATCGA CTGTAACCCA 
AAAGACAAAG ATAATTTCAA TATCGACCCC ATATGGATGT GCAAGGTTAG ACAAAGACAG 
ACGATCTCCC AGAAATCCCA AGAATCCCCC TCCGCTCTGA CGGCCTTCAC GAGCTCTGAT 
GCCCGCACCT TGCCACAGTT CCTGTCTGCC CATGAAAGAC AGGAATTTTC CTACGCTGCT 
GCTGTTCGTC GCTCACCGAA CCGCAGCCAT TTGACCCCCT GGGGTGACTC GCGCAGCTCC 
TCGCCAACCG CCAGCCTGTC AGCCAAACTT TCCCCACTTC GTCTGGGGTC AAAGGGCAGC 
AGCCCTTCCA GCACTACATC AGAGAGCACC TTAGAAAGGG ACAGAGAGCG CCCCCAGAGT 
GCCGGAGTGG TACAGGACTA TGGTAGAAAT CGCTACAATT TCTCAAATTT TGGTAGGGGG 
CAGGGCAGGG GCCAGGCATC TGCCACTTCT AGGGGCAACT ATTTTAATAA TAAAACCAGC 
AAAGCTGTAC GACAGCCAGG AAGGCCACGG TCCAACAGTT ACAGTGTCAC GCCACAGAAC 
TGCCCCACTA TGATACCTGG TATATTGTCT GTGACAGAAA ACCCCAGTGG AGAAAAAAAT 
GGGCCCAAAG CCAGTGATGG GGGGTGGATC AAAGTGGAGC GACAGAAGAG ATTAGCGCGT 
CAGGACAATA AACAAGTCAG AGGTCGGCAG AGGAGAGGGA GCAGGGGGGG GCGTGGAGCT 
GGTGGAAGAA CGGGGGCCCA CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k20 ( XM_011609656.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011607958.2 Takifugu rubripes mitogen-activated protein kinase kinase kinase 20 (map3k20), mRNA.

Price & Availability↑

CloneID	OTo42306
Clone ID Related Accession (Same CDS sequence)	XM_011609656.2
Accession Version	XM_011609656.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	mitogen-activated protein kinase kinase kinase 20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011609656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTCGC CAAGCGCCTC CTTCGTGCAA ATCAAGTTTG ATGACATCCT CTTCCACGAG 
AACTGCGGCG ACGGCAGCTT CGGAAGCGTG TACCGAGCCA GATGGATCTC CCAGGACAAA 
GAGGTGGCAG TGAAAAAACT GCTCAAGATT GAAAACGAGG CTGAAATCCT CAGCGTCCTC 
AGCCACCGTA ACATCATCCA GTTTTACGGC GCCGTTGTCG AGGCTCCCAA CTATGGAATC 
GTTACTGAGT ACGCCAGCGG TGGGTCGCTG TACGACTACC TGTCCAGTGC TGAGAGCGAA 
CGGATGGACA TGGGACAGAT CATGACGTGG GCTGCAGAGA TTGCCAGAGG AATGCATTAC 
TTACATTCAG AGGCCCCCGT TAAAGTCATC CACCGCGATT TAAAATCCAG GAACGTTGTG 
GTGACTGCAG ACAAAGTCCT GAAGATCTGT GATTTTGGGG CATCTAAGTT CCTGACACAC 
ACGACACACA TGTCGCTGGT GGGCACGTTT CCGTGGATGG CTCCGGAGGT GATCCAGAGC 
TTGCCTGTGT CTGAAACGTG TGACACCTTC TCCTTCGGTG TGGTTCTTTG GGAGATGCTC 
ACCAGTGAGA TACCCTTCAA AGGCCTGGAG GGTTTACAGG TGGCCTGGCT TGTGGTGGAG 
AAAAATGAGA GGTTAACCAT CCCGAGTGGC TGTCCTGCCA GTTTTGCTGA ACTCATGAGA 
AGCTGCTGGG CATCGGAGCC AAAAGACAGG CCAATGTTCA AGCAGATCCT CGCCACTTTG 
GAGTCCATGT CAAACGACAG CCAACTTCCT CAACAGTGTA ACTCCTTCCT GCACAACAAG 
GCTGAATGGA GGTGTGAGAT TGAAGCCACA CTTGAGAGAC TGAAAAAGCT GGAGAGAGAC 
CTGAGTACCA AAGAGCAGGA GCTGAAGGAG AGAGAGCAGC GTCTAAAGAT GTGGGAGCGG 
AAGCTCATCG AACAGTCCAG CAGCCCACTG CTGCCCACCC ATGACATCTA CACATGGACA 
GAGGAGCACG TGTATTTCTG GATGCAACAA ATATTTGGTG CAGAGGAGGG CCCCTGCGGC 
ATGCAGGTTT ACGCCAATCT GTTCAAAGAG AATCACATCA CCGGAAAGAG GTTACTGTTG 
CTCACAGAGA GCGACATGCG CGACATGGGG GTCAAGTCCA AAGGCCACGT CATGCATCTT 
AAGGGTGAAA TTGAGAAACT GACTCATGAC TATCTCAGCC TGGTCCATTT CCCTCCCCTG 
CTGAAGGAGG AACTGGAAAA GCAAGTGGAG ATGAGTAAAA CCATTAAATT GGAACTGGTG 
TTTGGGTACC ACTGGAAACC AGGAACAGGG AAAGATGACT GCAAGTGGAA AATGTACATC 
GAACTTGACG GGGACGATGT CGCCATCACC TACATCAAAG ATGTCGTCTT TAACACCAAC 
CGTGCTGACA TGGAAACGCT GCGCATGGCA AAGCCGCCCT TTGTGATGGA GAAATGGATC 
ACTGGCATAC AGCCGAACCA GCGGGTGGAC TACACTGTCA ATTATGAGAA TGATGTCAAA 
TTGCCAAAGT CTACCAGACA CAGCTGTCCA ATGACGTGGA GTCTCATTGG TGGACAGAAC 
CAGATCAAGA CAGTGGAGCT GGTCATTGAA ACCACACCAG CAAAAATCGA CTGTAACCCA 
AAAGACAAAG ATAATTTCAA TATCGACCCC ATATGGATGT GCAAGGTTAG ACAAAGACAG 
ACGATCTCCC AGAAATCCCA AGAATCCCCC TCCGCTCTGA CGGCCTTCAC GAGCTCTGAT 
GCCCGCACCT TGCCACAGTT CCTGTCTGCC CATGAAAGAC AGGAATTTTC CTACGCTGCT 
GCTGTTCGTC GCTCACCGAA CCGCAGCCAT TTGACCCCCT GGGGTGACTC GCGCAGCTCC 
TCGCCAACCG CCAGCCTGTC AGCCAAACTT TCCCCACTTC ATCTGGGGTC AAAGGGCAGC 
AGCCCTTCCA GCACTACATC AGAGAGCACC TTAGAAAGGG ACAGAGAGCG CCCCCAGAGT 
GCCGGAGTGG TACAGGACTA TGGTAGAAAT CGCTACAATT TCTCAAATTT TGGTAGGGGG 
CAGGGCAGGG GCCAGGCATC TGCCACTTCT AGGGGCAACT ATTTTAATAA TAAAACCAGC 
AAAGCTGTAC GACAGCCAGG AAGGCCACGG TCCAACAGTT ACAGTGTCAC GCCACAGAAC 
TGCCCCACTA TGATACCTGG TATATTGTCT GTGACAGAAA ACCCCAGTGG AGAAAAAAAT 
GGGCCCAAAG CCAGTGATGG GGGGTGGATC AAAGTGGAGC GACAGAAGAG ATTAGCGCGT 
CAGGACAATA AACAAGTCAG AGGTCGGCAG AGGAGAGGGA GCAGGGGGGG GCGTGGAGCT 
GGTGGAAGAA CGGGGGCCCA CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011607958.2
CDS	215..2641
Translation	MLSPSASFVQIKFDDILFHENCGDGSFGSVYRARWISQDKEVAVKKLLKIENEAEILSVLSHRNIIQFYGAVVEAPNYGIVTEYASGGSLYDYLSSAESERMDMGQIMTWAAEIARGMHYLHSEAPVKVIHRDLKSRNVVVTADKVLKICDFGASKFLTHTTHMSLVGTFPWMAPEVIQSLPVSETCDTFSFGVVLWEMLTSEIPFKGLEGLQVAWLVVEKNERLTIPSGCPASFAELMRSCWASEPKDRPMFKQILATLESMSNDSQLPQQCNSFLHNKAEWRCEIEATLERLKKLERDLSTKEQELKEREQRLKMWERKLIEQSSSPLLPTHDIYTWTEEHVYFWMQQIFGAEEGPCGMQVYANLFKENHITGKRLLLLTESDMRDMGVKSKGHVMHLKGEIEKLTHDYLSLVHFPPLLKEELEKQVEMSKTIKLELVFGYHWKPGTGKDDCKWKMYIELDGDDVAITYIKDVVFNTNRADMETLRMAKPPFVMEKWITGIQPNQRVDYTVNYENDVKLPKSTRHSCPMTWSLIGGQNQIKTVELVIETTPAKIDCNPKDKDNFNIDPIWMCKVRQRQTISQKSQESPSALTAFTSSDARTLPQFLSAHERQEFSYAAAVRRSPNRSHLTPWGDSRSSSPTASLSAKLSPLHLGSKGSSPSSTTSESTLERDRERPQSAGVVQDYGRNRYNFSNFGRGQGRGQASATSRGNYFNNKTSKAVRQPGRPRSNSYSVTPQNCPTMIPGILSVTENPSGEKNGPKASDGGWIKVERQKRLARQDNKQVRGRQRRGSRGGRGAGGRTGAHT

Target ORF information:

RefSeq Version	XM_011609656.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes mitogen-activated protein kinase kinase kinase 20 (map3k20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609656.2

ORF Insert Sequence:

ATGTTGTCGC CAAGCGCCTC CTTCGTGCAA ATCAAGTTTG ATGACATCCT CTTCCACGAG 
AACTGCGGCG ACGGCAGCTT CGGAAGCGTG TACCGAGCCA GATGGATCTC CCAGGACAAA 
GAGGTGGCAG TGAAAAAACT GCTCAAGATT GAAAACGAGG CTGAAATCCT CAGCGTCCTC 
AGCCACCGTA ACATCATCCA GTTTTACGGC GCCGTTGTCG AGGCTCCCAA CTATGGAATC 
GTTACTGAGT ACGCCAGCGG TGGGTCGCTG TACGACTACC TGTCCAGTGC TGAGAGCGAA 
CGGATGGACA TGGGACAGAT CATGACGTGG GCTGCAGAGA TTGCCAGAGG AATGCATTAC 
TTACATTCAG AGGCCCCCGT TAAAGTCATC CACCGCGATT TAAAATCCAG GAACGTTGTG 
GTGACTGCAG ACAAAGTCCT GAAGATCTGT GATTTTGGGG CATCTAAGTT CCTGACACAC 
ACGACACACA TGTCGCTGGT GGGCACGTTT CCGTGGATGG CTCCGGAGGT GATCCAGAGC 
TTGCCTGTGT CTGAAACGTG TGACACCTTC TCCTTCGGTG TGGTTCTTTG GGAGATGCTC 
ACCAGTGAGA TACCCTTCAA AGGCCTGGAG GGTTTACAGG TGGCCTGGCT TGTGGTGGAG 
AAAAATGAGA GGTTAACCAT CCCGAGTGGC TGTCCTGCCA GTTTTGCTGA ACTCATGAGA 
AGCTGCTGGG CATCGGAGCC AAAAGACAGG CCAATGTTCA AGCAGATCCT CGCCACTTTG 
GAGTCCATGT CAAACGACAG CCAACTTCCT CAACAGTGTA ACTCCTTCCT GCACAACAAG 
GCTGAATGGA GGTGTGAGAT TGAAGCCACA CTTGAGAGAC TGAAAAAGCT GGAGAGAGAC 
CTGAGTACCA AAGAGCAGGA GCTGAAGGAG AGAGAGCAGC GTCTAAAGAT GTGGGAGCGG 
AAGCTCATCG AACAGTCCAG CAGCCCACTG CTGCCCACCC ATGACATCTA CACATGGACA 
GAGGAGCACG TGTATTTCTG GATGCAACAA ATATTTGGTG CAGAGGAGGG CCCCTGCGGC 
ATGCAGGTTT ACGCCAATCT GTTCAAAGAG AATCACATCA CCGGAAAGAG GTTACTGTTG 
CTCACAGAGA GCGACATGCG CGACATGGGG GTCAAGTCCA AAGGCCACGT CATGCATCTT 
AAGGGTGAAA TTGAGAAACT GACTCATGAC TATCTCAGCC TGGTCCATTT CCCTCCCCTG 
CTGAAGGAGG AACTGGAAAA GCAAGTGGAG ATGAGTAAAA CCATTAAATT GGAACTGGTG 
TTTGGGTACC ACTGGAAACC AGGAACAGGG AAAGATGACT GCAAGTGGAA AATGTACATC 
GAACTTGACG GGGACGATGT CGCCATCACC TACATCAAAG ATGTCGTCTT TAACACCAAC 
CGTGCTGACA TGGAAACGCT GCGCATGGCA AAGCCGCCCT TTGTGATGGA GAAATGGATC 
ACTGGCATAC AGCCGAACCA GCGGGTGGAC TACACTGTCA ATTATGAGAA TGATGTCAAA 
TTGCCAAAGT CTACCAGACA CAGCTGTCCA ATGACGTGGA GTCTCATTGG TGGACAGAAC 
CAGATCAAGA CAGTGGAGCT GGTCATTGAA ACCACACCAG CAAAAATCGA CTGTAACCCA 
AAAGACAAAG ATAATTTCAA TATCGACCCC ATATGGATGT GCAAGGTTAG ACAAAGACAG 
ACGATCTCCC AGAAATCCCA AGAATCCCCC TCCGCTCTGA CGGCCTTCAC GAGCTCTGAT 
GCCCGCACCT TGCCACAGTT CCTGTCTGCC CATGAAAGAC AGGAATTTTC CTACGCTGCT 
GCTGTTCGTC GCTCACCGAA CCGCAGCCAT TTGACCCCCT GGGGTGACTC GCGCAGCTCC 
TCGCCAACCG CCAGCCTGTC AGCCAAACTT TCCCCACTTC ATCTGGGGTC AAAGGGCAGC 
AGCCCTTCCA GCACTACATC AGAGAGCACC TTAGAAAGGG ACAGAGAGCG CCCCCAGAGT 
GCCGGAGTGG TACAGGACTA TGGTAGAAAT CGCTACAATT TCTCAAATTT TGGTAGGGGG 
CAGGGCAGGG GCCAGGCATC TGCCACTTCT AGGGGCAACT ATTTTAATAA TAAAACCAGC 
AAAGCTGTAC GACAGCCAGG AAGGCCACGG TCCAACAGTT ACAGTGTCAC GCCACAGAAC 
TGCCCCACTA TGATACCTGG TATATTGTCT GTGACAGAAA ACCCCAGTGG AGAAAAAAAT 
GGGCCCAAAG CCAGTGATGG GGGGTGGATC AAAGTGGAGC GACAGAAGAG ATTAGCGCGT 
CAGGACAATA AACAAGTCAG AGGTCGGCAG AGGAGAGGGA GCAGGGGGGG GCGTGGAGCT 
GGTGGAAGAA CGGGGGCCCA CACATGA