Bovine

Enteropeptidase, ENTK, PRSS7

Reconstituted in sterile EK Storage Buffer (20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol) at 0.1 mg/ml

Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys(DDDDK↑). Enterokinase will not work if the recognition site is followed by a proline. rbEKhas the highest activity than EK of other species and is used wildly in biochemical applications. rbEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed to be removed from digestion system. Recombinant Bovine Enterokinase (His-tagged) (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids, 6 × His at C-terminus. A fully biologically active molecule, rbEK has a molecular mass of 40 kDa and is obtained by proprietary chromatographic techniques at GenScript.

S. pyogenes

E.coli

CBE4 is a custom-engineered fusion protein consisting of APOBEC3A, nCas9 (Cas9 nickase), and a nuclear localization signal (NLS). Designed as a cytidine base editor, it enables programmable, site-specific C-to-T conversions in genomic DNA, without inducing double-strand breaks (DSBs). APOBEC3A is a single-stranded DNA-specific cytidine deaminase that catalyzes C-to-U (cytosine to uracil) deamination. In the cellular context, the resulting U•G mismatch is resolved by DNA replication or repair machinery, ultimately leading to a C•G to T•A substitution. nCas9 guides the editor to the target site via an sgRNA while minimizing off-target double-strand cleavage. The NLS ensures efficient nuclear import, enabling effective editing in mammalian cells. Together, these components form a high-efficiency, low-indel base editing tool suitable for diverse genome engineering applications.

Human

E. coli

Expressed with an N-terminal Met.

Poly-His	IL-15 (Asn49-Ser162) Accession # P40933
N-term	C-term

IL15; IL-T; interleukin 15

Acidaminococcus sp. (strain BV3L6)

4(±10%) mg/ml

The clustered regularly interspaced short palindromic repeats, known as CRISPR systems are adaptive immune mechanisms commonly present in archaea and bacteria. The CRISPR systems enable the host to specifically target and cleave foreign nucleic acids thus targeting infectiousviruses and plasmids. Recently, a type V CRISPR system has been identified in several bacteria, the Cpf1 CRISPR from Prevotella and Francisella 1. In contrast to Cas9 systems, CRISPR/Cpf1 systems are smaller in size, do not require an additional trans-activating crRNA (tracrRNA), and allow for targeting of additional genomic regions by cleaveing the target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM). On the other hand, the Cas9 system requires a G-rich PAM following the target DNA. Furthermore, Cas12a/Cpf1 introduces a staggered DNA double stranded break with a 4 or 5-nt 5’ overhang. Recombinant Acidaminococcus sp. BV3L6 Cas12a (cpf1) nuclease is expressed in E. coli and purified. The nuclease contains nuclear localization sequence (NLS) at the C-terminus and 6× His-tag at the C-terminus.

Streptococcus pyogenes

4 mg/ml

GenCRISPR™ Cas9 v1.2 is a tag free nuclease produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes with a biparticle nucleus localization signal (BPNLS) at N-terminal and a nucleoplasmin nucleus localization signal (nucleoplasmin NLS) at C-terminal. It has been reported that BPNLS and nucleoplasmin NLS are able to improve the gene editing efficiency.

ELISA; WB;

Rabbit IgG

Horseradish peroxidase (HRP)

Biotin Antibody [HRP]; anti-Biotin Antibody [HRP]; HRP conjugated Biotin monoclonal antibody; MonoRab™ anti-Biotin Antibody [HRP]

ELISA; WB; WB;

Rabbit IgG

Unconjugated

RFP epitope tag peptide VNGHEFEIEGEGEGR conjugated to KLH

Staphylococcus aureus

Recombinant SaCas9 with an NLS at both N-terminal and C-terminal expressed by E.coli 

4 mg/ml

GenCRISPR™ SaCas9 2NLS Nuclease is a tag free nuclease produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Staphylococcus aureus with a nuclear localization signal at both N-terminal and C-terminal.The small size of the nuclease facilitates enhanced in vivo delivery for genome editing in various organisms.

ICC/IF; FCM;

Rabbit IgG

PE

Biotin Antibody [PE]; anti-Biotin Antibody [PE]; PE conjugated Biotin monoclonal antibody; MonoRab™ anti-Biotin Antibody [PE]

ICC/IF; FCM;

Rabbit IgG

iFluor 647

Biotin Antibody [iFluor 647]; anti-Biotin Antibody [iFluor 647]; iFluor 647 conjugated Biotin monoclonal antibody; MonoRab™ anti-Biotin Antibody [iFluor 647]