S. pyogenes

Recombinant Cas9-based fusion with three NLS expressed by E.coli

PE6C focuses on enhancing the performance of prime editing in transgenic rice plants. This specific variant of the prime editing technology is tailored to address the challenges of genetic improvement in crops, contributing to enhanced editing precision and efficiency in agricultural applications. The effectiveness of PE6c stems from its design improvements that enable it to facilitate targeted insertions and modifications in plant genomes.

Human

E. coli

Expressed with an N-terminal Met.

Poly-His	IL-15 (Asn49-Ser162) Accession # P40933
N-term	C-term

IL15; IL-T; interleukin 15

S. pyogenes

Recombinant Cas9-based fusion with three NLS expressed by E.coli

PE7 is a fusion protein of the small RNA-binding protein La to the prime editor. Research indicates that PE7 has demonstrated substantial improvements in intended editing efficiencies across various cell lines and genomic loci compared to previous versions like PEmax. This development holds promise for a wide range of applications in both basic research and therapeutic settings.

S. pyogenes

Recombinant Cas9-based fusion with three NLS expressed by E.coli

PE6b is a variant of the prime editor protein specifically designed for genome editing in living cells. It enhances the efficiency of genome edits compared to earlier versions of prime editors. Notably, editors like PE6b are smaller in size, allowing for more efficient delivery when used in medical and therapeutic applications.

Acidaminococcus sp. (strain BV3L6)

4(±10%) mg/ml

The clustered regularly interspaced short palindromic repeats, known as CRISPR systems are adaptive immune mechanisms commonly present in archaea and bacteria. The CRISPR systems enable the host to specifically target and cleave foreign nucleic acids thus targeting infectiousviruses and plasmids. Recently, a type V CRISPR system has been identified in several bacteria, the Cpf1 CRISPR from Prevotella and Francisella 1. In contrast to Cas9 systems, CRISPR/Cpf1 systems are smaller in size, do not require an additional trans-activating crRNA (tracrRNA), and allow for targeting of additional genomic regions by cleaveing the target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM). On the other hand, the Cas9 system requires a G-rich PAM following the target DNA. Furthermore, Cas12a/Cpf1 introduces a staggered DNA double stranded break with a 4 or 5-nt 5’ overhang. Recombinant Acidaminococcus sp. BV3L6 Cas12a (cpf1) nuclease is expressed in E. coli and purified. The nuclease contains nuclear localization sequence (NLS) at the C-terminus and 6× His-tag at the C-terminus.

S. pyogenes

Recombinant Cas9-based fusion with three NLS expressed by E.coli

PEmax RNase deletion involves modifications to the PEmax enzyme aimed at eliminating unwanted RNase activity, thus enhancing its stability and specificity. This advancement allows for more efficient target editing by minimizing the degradation of RNA components involved in the editing process, further improving the overall performance of the prime editing protocol.

Streptococcus pyogenes

4 mg/ml

GenCRISPR™ Cas9 v1.2 is a tag free nuclease produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes with a biparticle nucleus localization signal (BPNLS) at N-terminal and a nucleoplasmin nucleus localization signal (nucleoplasmin NLS) at C-terminal. It has been reported that BPNLS and nucleoplasmin NLS are able to improve the gene editing efficiency.

ELISA; WB;

Rabbit IgG

Horseradish peroxidase (HRP)

Biotin Antibody [HRP]; anti-Biotin Antibody [HRP]; HRP conjugated Biotin monoclonal antibody; MonoRab™ anti-Biotin Antibody [HRP]

S. pyogenes

Recombinant Cas9-based fusion with three NLS expressed by E.coli

PE6d is designed to optimize the efficiency of prime editing by altering certain components of the prime editor framework. It incorporates elements that minimize the chances of errors and maximize successful genomic edits across various cell types. This improvement results in enhanced editing accuracy and reduced byproducts, such as unintended indels.

ELISA; WB; WB;

Rabbit IgG

Unconjugated

RFP epitope tag peptide VNGHEFEIEGEGEGR conjugated to KLH