ICC/IF; FCM;

Rabbit IgG

Fluorescein (FITC)

DYKDDDDK synthetic peptide coupled to KLH

ELISA; WB; ICC/IF;

Rabbit IgG

Unconjugated

a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA). conjugated to KLH

ELISA; WB;

Rabbit IgG

Biotin

DYKDDDDK synthetic peptide coupled to KLH

ELISA; WB;

Rabbit IgG

Horseradish peroxidase (HRP)

DYKDDDDK synthetic peptide coupled to KLH

WB; FCM; ELISA;

Rabbit IgG, κ

Unconjugated

A synthetic peptide HHHHHH coupled to KLH

ELISA; WB; IP;

Rabbit IgG

Unconjugated

DYKDDDDK synthetic peptide coupled to KLH

SARS-CoV-2

CHO

Expressed with the mutations of E484K, K417N, N501Y.

Spike RBD (Arg319-Ser591) Accession # P0DTC2	Avi	Poly-His
N-term		C-term

Lineage P.1; 20J/501Y.V3; VOC-202101/02; Brazil(ian) variant; Gamma variant

GenCrispr NLS-Cas9-EGFP is a fusion protein developed by GenScript. It contains a nuclear localization signal (NLS) on its N terminal end, and an EGFP and a 6x(His) sequence on the C terminal end. Cas9 nuclease is an RNA-guided endonuclease that can catalyze cleavage of double stranded DNA. This kind of targeted nuclease is a powerful tool for genome editing with high precision. Cas9 protein forms a highly stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. When Cas9 is expressed with an NLS sequence, the Cas9 RNP complex can localize to the nucleus immediately upon entering the cell. There is no requirement for in vivo transcription or translation, which improves the efficiency of this method over plasmid-based systems. Additionally, the Cas9 RNP complex is rapidly cleared from the cells minimizing the chance of off-target cleavage when compared to other systems (Kim, et al. 2014). The EGFP tag can be used as a reporter for tracking or sorting transfected cells, which enables enrichment of cell populations for desired genome edits via fluorescence activated cell sorting (FACS). It significantly reduces the labor and cost associated with single cell cloning and genotyping in genome editing applications. 

Human

E. coli

Expressed with additional Leu-Glu at C-terminal.

HMGB1 (Met1-Glu215) Accession # P09429	Poly-His
N-term	C-term

HMGB1; HMG-1; High mobility group protein 1; HMG1; High mobility group protein B1

Bovine

Enteropeptidase, ENTK, PRSS7

Reconstituted in sterile EK Storage Buffer (20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol) at 0.1 mg/ml

Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys(DDDDK↑). Enterokinase will not work if the recognition site is followed by a proline. rbEKhas the highest activity than EK of other species and is used wildly in biochemical applications. rbEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed to be removed from digestion system. Recombinant Bovine Enterokinase (His-tagged) (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids, 6 × His at C-terminus. A fully biologically active molecule, rbEK has a molecular mass of 40 kDa and is obtained by proprietary chromatographic techniques at GenScript.