ELISA; WB; IP; FCM; ICC/IF;

Mouse IgG2a,κ

Unconjugated

V5 tag peptide conjugated to KLH

WB; IP; ICC/IF; ELISA; FCM;

Mouse IgG1,κ

Unconjugated

NWSHPQFEK peptide conjugated to KLH

ELISA; WB;

Rabbit IgG

Horseradish peroxidase (HRP)

c-Myc epitope tag peptide EQKLISEEDL conjugated to KLH

GenScript’s MonoRabᵀᴹ Anti-DYKDDDDK Affinity Resin is designed for optimal purification of DYKDDDDK-tagged proteins. DYKDDDDK tag is an octapeptide tag that can be added to a protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification. Based on our new rabbit monoclonal antibody technology (MonoRabᵀᴹ), this resin offers high binding capacity, high purity, and resistance to high concentrations of salt or acid during protein purification. The binding capacity of MonoRabᵀᴹ Anti-DYKDDDDK Affinity Resin is 1.5 mg DYKDDDDK-tagged protein per mL of settled resin. The recombinant proteins expressed in bacterial or mammalian cells cultures specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin, resulting in highly pure eluates. The resin can withstand stringent washing steps, especially with buffers containing high concentration of salt. The resin is resistant to up to 2 M NaCl, and to buffers with pH as low as 2.5.

ICC/IF; FCM;

Mouse IgG2b,κ

iFluor 647

A synthetic peptide DYKDDDDK coupled to KLH

ICC/IF; FCM;

Mouse IgG1,κ

iFluor 488

A synthetic peptide YPYDVPDYA coupled to KLH

ICC/IF; FCM;

Mouse IgG1,κ

iFluor 647

A synthetic peptide YPYDVPDYA coupled to KLH

Human Rhinovirus

rPSP, PSP, HRV 3C Protease

Recombinant PreScission Protease (rPSP) from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. PSP is a highly purified recombinant 6xHis-fusion protein, which recognizes the same cleavage site as the native enzyme. It is used to cleave affinity tags from fusion proteins. The optimum recognition site for this enzyme is the sequence Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro(LEVLFQ/GP) and cleavage occurs between the Gln and Gly-Pro residues. The optimal temperature for cleavage is 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant PreScission Protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.Recombinant PreScission Protease (rPSP) contains 217 amino acids with N-terminal His tag. A fully biologically active molecule, rPSP has a molecular mass of 24 kDa and is obtained by proprietary chromatographic techniques at GenScript.

ELISA; WB; IP; ICC/IF; FCM;

Rabbit IgG

Unconjugated

6x His synthetic peptide coupled to KLH

ELISA; WB;

Mouse IgG2a,κ

Biotin

V5 tag peptide conjugated to KLH