Bovine

Enteropeptidase, ENTK, PRSS7

Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. rbEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.

S. pyogenes

Recombinant Cas9-based fusion with three NLS expressed by E.coli

PE6d is designed to optimize the efficiency of prime editing by altering certain components of the prime editor framework. It incorporates elements that minimize the chances of errors and maximize successful genomic edits across various cell types. This improvement results in enhanced editing accuracy and reduced byproducts, such as unintended indels.

S. pyogenes

Recombinant Cas9-based fusion with three NLS expressed by E.coli

PEmax is an improved version of PE2 with additional NLS and improved nCas9 domain, facilitating precise genome editing without the need for double-stranded DNA breaks. It significantly increases editing efficiency and accuracy compared to earlier systems and allows for a broader range of base conversions and edits within the genome design

S. pyogenes

Recombinant Cas9-based fusion with three NLS expressed by E.coli

PE6b is a variant of the prime editor protein specifically designed for genome editing in living cells. It enhances the efficiency of genome edits compared to earlier versions of prime editors. Notably, editors like PE6b are smaller in size, allowing for more efficient delivery when used in medical and therapeutic applications.

Acidaminococcus sp. (strain BV3L6)

4(±10%) mg/ml

The clustered regularly interspaced short palindromic repeats, known as CRISPR systems are adaptive immune mechanisms commonly present in archaea and bacteria. The CRISPR systems enable the host to specifically target and cleave foreign nucleic acids thus targeting infectiousviruses and plasmids. Recently, a type V CRISPR system has been identified in several bacteria, the Cpf1 CRISPR from Prevotella and Francisella 1. In contrast to Cas9 systems, CRISPR/Cpf1 systems are smaller in size, do not require an additional trans-activating crRNA (tracrRNA), and allow for targeting of additional genomic regions by cleaveing the target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM). On the other hand, the Cas9 system requires a G-rich PAM following the target DNA. Furthermore, Cas12a/Cpf1 introduces a staggered DNA double stranded break with a 4 or 5-nt 5’ overhang. Recombinant Acidaminococcus sp. BV3L6 Cas12a (cpf1) nuclease is expressed in E. coli and purified. The nuclease contains nuclear localization sequence (NLS) at the C-terminus and 6× His-tag at the C-terminus.

S. pyogenes

Recombinant Cas9-based fusion with double-ended NLS expressed by E.coli

ABE8e is an innovative adenine base editor designed to enable precise genetic modifications by converting A-T base pairs to G-C base pairs without introducing double-stranded breaks or requiring donor DNA templates.​ ABE8e contains eight additional mutations in the TadA domain, augments the effectiveness and applicability of adenine base editing.

Eubacterium rectale

4 mg/ml

GenCRISPR™ ErCas12a Nuclease is an RNA-guided DNA endonuclease from Eubacterium rectale. It recognizes a T-rich protospacer adjacent motif (PAM) and results in a staggered DNA double-strand break (DSB). After the specific cleavage, Cas12a can also activate collateral cleavage activity towards adjacent non-specific ssDNA sequences. Hence, Cas12a nuclease is a good alternative for Cas9 in certain target DNA editing, and provides a novel strategy for DNA detection.

Lachnospiraceae bacterium

4 mg/ml

GenCRISPR™ LbCas12a Nuclease is an RNA-guided DNA endonuclease from Lachnospiraceae bacterium. Cas12-family enzymes exhibit two DNase activity modes; besides crRNA-guided cis-cleavage of cDNA targets, these nucleases also catalyze trans-cleavage of non-target ssDNA substrates introduced by target DNA, which has been exploited to develop sensitive technologies for nucleic acid detection.

Leptotrichia wadei

4 mg/ml

GenCRISPR Cas13a (C2c2) Nuclease is an RNA-guided, RNA-targeting CRISPR enzyme, commonly referred to as C2c2. This enzyme is a member of Type VI CRISPR family containing a single protein effector with two HEPN domains. The Cas13a exhibits two RNase activities. The first activity, a cis- sequence-specific RNA guided cleavage, activates the secondary RNase activity of the enzyme, a non-specific trans- ribonuclease activity. This Cas13a protein can be applied to detect single RNA molecules with proven high specificity.

Canine

E.coli

TAG-72 (Met1-Gln478) Accession # XP_005615459.2	His
N-term	C-term

mRNA (guanine-N(7))-methyltransferase; mRNA-methyltransferase; TAG 72; TAG72