His-taghis tagged; epitope tag

The amino acid sequence consisting of 6 His residues in a row, called His-Tag, would act as a metal binding site for a recombinant protein. His-Tag sequence can be placed on the N- or -terminal of a target protein by using vectors from various commercial molecular biology companies. And this Tag is often followed by a cleavage site for a specific protease - for example Leu-Val-Arg-Gly-Ser peptide sequence is recognized and cleaved by the protease known as thrombin.

SARS-CoV-2

CHO

Expressed with the mutations of T19R, G142D, del 156-157, R158G, L452R, T478K, D614G, P681R.

S1 protein (Gln14-Arg685) Accession # P0DTC2	Poly-His
N-term	C-term

Lineage B.1.617.2; VUI-21APR-02; variant Delta

Human Rhinovirus

rPSP, PSP, HRV 3C Protease

Recombinant PreScission Protease (rPSP) from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. PSP is a highly purified recombinant 6xHis-fusion protein, which recognizes the same cleavage site as the native enzyme. It is used to cleave affinity tags from fusion proteins. The optimum recognition site for this enzyme is the sequence Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro(LEVLFQ/GP) and cleavage occurs between the Gln and Gly-Pro residues. The optimal temperature for cleavage is 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant PreScission Protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.Recombinant PreScission Protease (rPSP) contains 217 amino acids with N-terminal His tag. A fully biologically active molecule, rPSP has a molecular mass of 24 kDa and is obtained by proprietary chromatographic techniques at GenScript.

Human

E. coli

Expressed with an N-terminal Met.

Poly-His	TNF-α (Val77-Leu233) Accession # P01375
N-term	C-term

TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; DIF; TNFA; TNFSF2

Bovine

GenScript Enterokinase Animal-Free, His, Bovine is the light chain of recombinant bovine enterokinase that is a single glycosylated polypeptide chain containing 200 amino acids and is produced under animal free process, which is more friendly and safer for drug or vaccine development, manufacture and other applications. This product is designed with a 6 × His tag for further removing from the digestion system by being captured by Ni2+ affinity chromatography.

SARS-CoV-2

CHO

S1 protein (Val16-Arg685) Accession # P0DTC2	Poly-His
N-term	C-term

SARS-CoV-2 S1 protein; 2019-nCoV S1 protein

SARS-CoV-2

Human Cells

Spike RBD (Arg319-Ser591) Accession # P0DTC2	Avi	Poly-His
N-term		C-term

HRP

Human

E. coli

Expressed with additional Leu-Glu at C-terminal.

HMGB1 (Met1-Glu215) Accession # P09429	Poly-His
N-term	C-term

HMGB1; HMG-1; High mobility group protein 1; HMG1; High mobility group protein B1

GenScript High Affinity Ni-Charged Resin is an 4% cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins.

Bovine

Enteropeptidase, ENTK, PRSS7

Reconstituted in sterile EK Storage Buffer (20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol) at 0.1 mg/ml

Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys(DDDDK↑). Enterokinase will not work if the recognition site is followed by a proline. rbEKhas the highest activity than EK of other species and is used wildly in biochemical applications. rbEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed to be removed from digestion system. Recombinant Bovine Enterokinase (His-tagged) (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids, 6 × His at C-terminus. A fully biologically active molecule, rbEK has a molecular mass of 40 kDa and is obtained by proprietary chromatographic techniques at GenScript.