LOC105217199 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105217199
Entrez Gene ID	105217199
Full Name	proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011192096.2	XP_011190398.1	26S proteasome non-ATPase regulatory subunit 2
XM_011192096.1	XP_011190398.1	26S proteasome non-ATPase regulatory subunit 2

Pathways from BioSystems
Gene Ontology

The following LOC105217199 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105217199 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa192343	XM_011192096.2 Latest version!	Zeugodacus cucurbitae 26S proteasome non-ATPase regulatory subunit 2 (LOC105217199), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OBa192343	XM_011192096.1	Bactrocera cucurbitae 26S proteasome non-ATPase regulatory subunit 2 (LOC105217199), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105217199 ( XM_011192096.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011190398.1 Zeugodacus cucurbitae 26S proteasome non-ATPase regulatory subunit 2 (LOC105217199), mRNA.

Price & Availability↑

CloneID	OBa192343
Clone ID Related Accession (Same CDS sequence)	XM_011192096.2 , XM_011192096.1
Accession Version	XM_011192096.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	26S proteasome non-ATPase regulatory subunit 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863667.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011192096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGCTC CCAAGAAAGA TGAAAAAACT TCCGCTGAGG TGAAGAAAGA CCAAGTGAAG 
GAGACACCGG AAAAAGAAGC AAATAAAGAT GCTAAAGACG AAGGTAAGGA GCAGGAGCTT 
TCCGATGAGG ATCAACAGCT GCAAGATGAA CTTGAAATGC TGGTTAATCG CCTACAAGAG 
GCCGATAAGG CATTGTATTT ACCAGCATTG GAAATGTTGG CAAAACTTAT ACGTGCATCT 
ACTACATCTA TGACTTCTGT ACCGAAACCA TTGAAGTTTA TGCGTCCCCA TTATGAGACT 
ATGAAAACAA TATACAAACA AATGCCTGAT CAGGAAACTC GGCAATTGTG CGCTGACATC 
ATATCCGTGC TGTCAATGAC AATGGGAAGT GGTAAGGATT GTTTGGCTTA CCGTTTTCTT 
TGTGATCGTT CGCAGAAAAT CGGTGATTGG GGTCACGAAT ATGTGCGCCA TTTGTCTGGA 
GAAATAGCTT CGCACTATTT GGATACATCA GGAGAATTTC AGGCACAGCT AATCGAGTTG 
GTGAAACAAA TAATCCCATA CAATATGGAA CACAATGCCG AGGCTGATGC TTGCGATTTA 
TTAATTGAGA TCGATCATTT GCATTTGTTG CAAGATTATG TCGATGAATC CGCTTATCCA 
CGCGTCTGTT TATACTTACA ATCTTGCTAT CCATATGTAC CCGAACCGGA TAATACTATA 
ATTCTGGAAA CAGCGCTACA GTTGGCACGT AAATTTAATC AACATACACA AGCGCTGCGT 
CTAGCATTGA TGTTAAATGA TATGGATAAA ATTTCCGAAA TATTCAAGGA ACCTAAAGAT 
GCAGCTGTGC AAAAACAGCT TGCTTTTATG TTAGCACGTC AGCAAGTATG CTTGGAATTA 
GATGAGTCGG TGCCGGATTA TGATGATTTA ATGGAAATCA TGTCTAACAC GAACTTGAAT 
AAACATTTTT TGAATTTGGC ACGTGAACTT GATATAATGG AAGCCAAGAC ACCAGAAGAC 
ATTTACAAAT CGCATTTGGA TAATGCGCGC ACTCGTTTCG CTTCAATACA GGTTGATTCG 
GCGAAACAGA ATTTGGCTGC CAGTTTTGTG AATGGTTTTG TTAACGCTGG CTTTGGTGTG 
GACAAACTAC TCTCTGAAGA TGGCAATAAG TGGTTGTATA AAAATAAAGA GCATGGCATG 
CTTTCAGCAA CTGCTTCCTT GGGACTCATT CTATTATGGG ATGTTGATGG TGGCTTAACT 
ATGATTGACA AATATCTGTA TTCTACCGAT GATTACATCA AATCCGGTGC TTTACTAGCC 
TGTGGTATTG TTAATTGTGG CATACGTAAT GAGGTCGACC CGGCGCATGC GCTACTCTCC 
GATTATATTG ACAACCAGAA TACTTCAATG CGTATCGGCG CCATTTTGGG TCTGGGTATA 
GCATATGCCG GCTCTAATCG TTCCATTGTC ATAGACACAC TAAAAACAGT ATTTGTGTCT 
AATGGTAAAA ATGGATCCAA TGTGGAAATC ATGGGTATAG CTGCACTATC GTTGGGCTTA 
ATATCAGTGG GTTCCTGCAA TGCAGAAATC ACGGAAATCT TACTACAAAC CATCATGGGT 
CTCTCTAAGT CTGATCTCAA AGACACCTAC TCACGTTTCT TGTGGTTAGG CTTGGGGCTA 
TTGTATTTGG GACGCCAGAA AGCCACCGAA GCCGTTATGC TCACCTTAGA GGTACTTGAT 
GAACCATATC GTTCAATGGC CACAACTATG GTGGACATTT GTGCCTACGC AGGCACTGGA 
AATGTTTTGA AAATACAGCA GTTGTTGCAT ATTTGCTCCG ATCATTACGA ATCTGCTAAC 
GGTGATGGTG CCGATGAAAA GGGCAAGAAG GATAAAAATA AGGAAAAGGA TAAGGCCGAA 
AAAGAGAAGG AAAAGGAGAA AGATCTATCC GCAACGCAAG CTATCGCTGT ATTAGGTATT 
GCATTAATTG CTATGGGTGA AGACATTGGT GCCGAGATGG CTTTCCGCTC ATTCGGTAAT 
TTACTACGCT ATTGCGAGCC CTGTGTTCGT CGTGCAGTGC CCCTAGCTCT GGGTCTAATC 
TCTGCGTCCA ATCCAAAATT GAATATTTTG GATACGTTAA GTAAATTCTC GCACGACAGT 
GATGCCGAAG TTGCACATAA TGCCATTTTT GCAATGGGTC TTATCGGTGC TGGCACGAAC 
AATGCACGTT TAGCATCTAT GTTGCGTCAA TTGGCCCAAT ATCATTCAAA GGATCCCAGC 
AATTTGTTTA TGGTACGAAT TGCACAAAGT TTAACACATT TGGGTAAAGG CACATTGACA 
CTCAGTCCTT ACCATAGTGA TCGGCAGTTG ATGAATCCAA TGGCTGTCGC CGGTCTGATG 
GCGACTTTAG TGTCTTTGCT AGATGTTAAA ACCTTAATAT TGGGTCGCTC ACACTACCTA 
CTGTACACGT TAGTGCCAGC CATGCAGGCA CGCATGCTAA TCACTTTTGA TGAAGAACTG 
AATCAACTGC AAGTGCCGGT GCGTGTCGGC ATTGCCATTG ATGTGGTGGG ACAAGCTGGT 
AAACCTAAAA CCATTACCGG TTTCCAAACT CATACAACAC CTGTACTGCT CGCGATGGGT 
GAACGCGCTG AATTGGCCAC AGATGAGTAC ATTTCGCTTA CACCCGTAAT GGAAGGATTT 
GTCATATTGA AGAAAAACCC AAACTTTGTG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011190398.1
CDS	164..2899
Translation	MVAPKKDEKTSAEVKKDQVKETPEKEANKDAKDEGKEQELSDEDQQLQDELEMLVNRLQEADKALYLPALEMLAKLIRASTTSMTSVPKPLKFMRPHYETMKTIYKQMPDQETRQLCADIISVLSMTMGSGKDCLAYRFLCDRSQKIGDWGHEYVRHLSGEIASHYLDTSGEFQAQLIELVKQIIPYNMEHNAEADACDLLIEIDHLHLLQDYVDESAYPRVCLYLQSCYPYVPEPDNTIILETALQLARKFNQHTQALRLALMLNDMDKISEIFKEPKDAAVQKQLAFMLARQQVCLELDESVPDYDDLMEIMSNTNLNKHFLNLARELDIMEAKTPEDIYKSHLDNARTRFASIQVDSAKQNLAASFVNGFVNAGFGVDKLLSEDGNKWLYKNKEHGMLSATASLGLILLWDVDGGLTMIDKYLYSTDDYIKSGALLACGIVNCGIRNEVDPAHALLSDYIDNQNTSMRIGAILGLGIAYAGSNRSIVIDTLKTVFVSNGKNGSNVEIMGIAALSLGLISVGSCNAEITEILLQTIMGLSKSDLKDTYSRFLWLGLGLLYLGRQKATEAVMLTLEVLDEPYRSMATTMVDICAYAGTGNVLKIQQLLHICSDHYESANGDGADEKGKKDKNKEKDKAEKEKEKEKDLSATQAIAVLGIALIAMGEDIGAEMAFRSFGNLLRYCEPCVRRAVPLALGLISASNPKLNILDTLSKFSHDSDAEVAHNAIFAMGLIGAGTNNARLASMLRQLAQYHSKDPSNLFMVRIAQSLTHLGKGTLTLSPYHSDRQLMNPMAVAGLMATLVSLLDVKTLILGRSHYLLYTLVPAMQARMLITFDEELNQLQVPVRVGIAIDVVGQAGKPKTITGFQTHTTPVLLAMGERAELATDEYISLTPVMEGFVILKKNPNFVK

Target ORF information:

RefSeq Version	XM_011192096.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae 26S proteasome non-ATPase regulatory subunit 2 (LOC105217199), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011192096.2

ORF Insert Sequence:

ATGGTTGCTC CCAAGAAAGA TGAAAAAACT TCCGCTGAGG TGAAGAAAGA CCAAGTGAAG 
GAGACACCGG AAAAAGAAGC AAATAAAGAT GCTAAAGACG AAGGTAAGGA GCAGGAGCTT 
TCCGATGAGG ATCAACAGCT GCAAGATGAA CTTGAAATGC TGGTTAATCG CCTACAAGAG 
GCCGATAAGG CATTGTATTT ACCAGCATTG GAAATGTTGG CAAAACTTAT ACGTGCATCT 
ACTACATCTA TGACTTCTGT ACCGAAACCA TTGAAGTTTA TGCGTCCCCA TTATGAGACT 
ATGAAAACAA TATACAAACA AATGCCTGAT CAGGAAACTC GGCAATTGTG CGCTGACATC 
ATATCCGTGC TGTCAATGAC AATGGGAAGT GGTAAGGATT GTTTGGCTTA CCGTTTTCTT 
TGTGATCGTT CGCAGAAAAT CGGTGATTGG GGTCACGAAT ATGTGCGCCA TTTGTCTGGA 
GAAATAGCTT CGCACTATTT GGATACATCA GGAGAATTTC AGGCACAGCT AATCGAGTTG 
GTGAAACAAA TAATCCCATA CAATATGGAA CACAATGCCG AGGCTGATGC TTGCGATTTA 
TTAATTGAGA TCGATCATTT GCATTTGTTG CAAGATTATG TCGATGAATC CGCTTATCCA 
CGCGTCTGTT TATACTTACA ATCTTGCTAT CCATATGTAC CCGAACCGGA TAATACTATA 
ATTCTGGAAA CAGCGCTACA GTTGGCACGT AAATTTAATC AACATACACA AGCGCTGCGT 
CTAGCATTGA TGTTAAATGA TATGGATAAA ATTTCCGAAA TATTCAAGGA ACCTAAAGAT 
GCAGCTGTGC AAAAACAGCT TGCTTTTATG TTAGCACGTC AGCAAGTATG CTTGGAATTA 
GATGAGTCGG TGCCGGATTA TGATGATTTA ATGGAAATCA TGTCTAACAC GAACTTGAAT 
AAACATTTTT TGAATTTGGC ACGTGAACTT GATATAATGG AAGCCAAGAC ACCAGAAGAC 
ATTTACAAAT CGCATTTGGA TAATGCGCGC ACTCGTTTCG CTTCAATACA GGTTGATTCG 
GCGAAACAGA ATTTGGCTGC CAGTTTTGTG AATGGTTTTG TTAACGCTGG CTTTGGTGTG 
GACAAACTAC TCTCTGAAGA TGGCAATAAG TGGTTGTATA AAAATAAAGA GCATGGCATG 
CTTTCAGCAA CTGCTTCCTT GGGACTCATT CTATTATGGG ATGTTGATGG TGGCTTAACT 
ATGATTGACA AATATCTGTA TTCTACCGAT GATTACATCA AATCCGGTGC TTTACTAGCC 
TGTGGTATTG TTAATTGTGG CATACGTAAT GAGGTCGACC CGGCGCATGC GCTACTCTCC 
GATTATATTG ACAACCAGAA TACTTCAATG CGTATCGGCG CCATTTTGGG TCTGGGTATA 
GCATATGCCG GCTCTAATCG TTCCATTGTC ATAGACACAC TAAAAACAGT ATTTGTGTCT 
AATGGTAAAA ATGGATCCAA TGTGGAAATC ATGGGTATAG CTGCACTATC GTTGGGCTTA 
ATATCAGTGG GTTCCTGCAA TGCAGAAATC ACGGAAATCT TACTACAAAC CATCATGGGT 
CTCTCTAAGT CTGATCTCAA AGACACCTAC TCACGTTTCT TGTGGTTAGG CTTGGGGCTA 
TTGTATTTGG GACGCCAGAA AGCCACCGAA GCCGTTATGC TCACCTTAGA GGTACTTGAT 
GAACCATATC GTTCAATGGC CACAACTATG GTGGACATTT GTGCCTACGC AGGCACTGGA 
AATGTTTTGA AAATACAGCA GTTGTTGCAT ATTTGCTCCG ATCATTACGA ATCTGCTAAC 
GGTGATGGTG CCGATGAAAA GGGCAAGAAG GATAAAAATA AGGAAAAGGA TAAGGCCGAA 
AAAGAGAAGG AAAAGGAGAA AGATCTATCC GCAACGCAAG CTATCGCTGT ATTAGGTATT 
GCATTAATTG CTATGGGTGA AGACATTGGT GCCGAGATGG CTTTCCGCTC ATTCGGTAAT 
TTACTACGCT ATTGCGAGCC CTGTGTTCGT CGTGCAGTGC CCCTAGCTCT GGGTCTAATC 
TCTGCGTCCA ATCCAAAATT GAATATTTTG GATACGTTAA GTAAATTCTC GCACGACAGT 
GATGCCGAAG TTGCACATAA TGCCATTTTT GCAATGGGTC TTATCGGTGC TGGCACGAAC 
AATGCACGTT TAGCATCTAT GTTGCGTCAA TTGGCCCAAT ATCATTCAAA GGATCCCAGC 
AATTTGTTTA TGGTACGAAT TGCACAAAGT TTAACACATT TGGGTAAAGG CACATTGACA 
CTCAGTCCTT ACCATAGTGA TCGGCAGTTG ATGAATCCAA TGGCTGTCGC CGGTCTGATG 
GCGACTTTAG TGTCTTTGCT AGATGTTAAA ACCTTAATAT TGGGTCGCTC ACACTACCTA 
CTGTACACGT TAGTGCCAGC CATGCAGGCA CGCATGCTAA TCACTTTTGA TGAAGAACTG 
AATCAACTGC AAGTGCCGGT GCGTGTCGGC ATTGCCATTG ATGTGGTGGG ACAAGCTGGT 
AAACCTAAAA CCATTACCGG TTTCCAAACT CATACAACAC CTGTACTGCT CGCGATGGGT 
GAACGCGCTG AATTGGCCAC AGATGAGTAC ATTTCGCTTA CACCCGTAAT GGAAGGATTT 
GTCATATTGA AGAAAAACCC AAACTTTGTG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217199 ( XM_011192096.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011190398.1 Bactrocera cucurbitae 26S proteasome non-ATPase regulatory subunit 2 (LOC105217199), mRNA.

Price & Availability↑

CloneID	OBa192343
Clone ID Related Accession (Same CDS sequence)	XM_011192096.2 , XM_011192096.1
Accession Version	XM_011192096.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	26S proteasome non-ATPase regulatory subunit 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863667.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTGCTC CCAAGAAAGA TGAAAAAACT TCCGCTGAGG TGAAGAAAGA CCAAGTGAAG 
GAGACACCGG AAAAAGAAGC AAATAAAGAT GCTAAAGACG AAGGTAAGGA GCAGGAGCTT 
TCCGATGAGG ATCAACAGCT GCAAGATGAA CTTGAAATGC TGGTTAATCG CCTACAAGAG 
GCCGATAAGG CATTGTATTT ACCAGCATTG GAAATGTTGG CAAAACTTAT ACGTGCATCT 
ACTACATCTA TGACTTCTGT ACCGAAACCA TTGAAGTTTA TGCGTCCCCA TTATGAGACT 
ATGAAAACAA TATACAAACA AATGCCTGAT CAGGAAACTC GGCAATTGTG CGCTGACATC 
ATATCCGTGC TGTCAATGAC AATGGGAAGT GGTAAGGATT GTTTGGCTTA CCGTTTTCTT 
TGTGATCGTT CGCAGAAAAT CGGTGATTGG GGTCACGAAT ATGTGCGCCA TTTGTCTGGA 
GAAATAGCTT CGCACTATTT GGATACATCA GGAGAATTTC AGGCACAGCT AATCGAGTTG 
GTGAAACAAA TAATCCCATA CAATATGGAA CACAATGCCG AGGCTGATGC TTGCGATTTA 
TTAATTGAGA TCGATCATTT GCATTTGTTG CAAGATTATG TCGATGAATC CGCTTATCCA 
CGCGTCTGTT TATACTTACA ATCTTGCTAT CCATATGTAC CCGAACCGGA TAATACTATA 
ATTCTGGAAA CAGCGCTACA GTTGGCACGT AAATTTAATC AACATACACA AGCGCTGCGT 
CTAGCATTGA TGTTAAATGA TATGGATAAA ATTTCCGAAA TATTCAAGGA ACCTAAAGAT 
GCAGCTGTGC AAAAACAGCT TGCTTTTATG TTAGCACGTC AGCAAGTATG CTTGGAATTA 
GATGAGTCGG TGCCGGATTA TGATGATTTA ATGGAAATCA TGTCTAACAC GAACTTGAAT 
AAACATTTTT TGAATTTGGC ACGTGAACTT GATATAATGG AAGCCAAGAC ACCAGAAGAC 
ATTTACAAAT CGCATTTGGA TAATGCGCGC ACTCGTTTCG CTTCAATACA GGTTGATTCG 
GCGAAACAGA ATTTGGCTGC CAGTTTTGTG AATGGTTTTG TTAACGCTGG CTTTGGTGTG 
GACAAACTAC TCTCTGAAGA TGGCAATAAG TGGTTGTATA AAAATAAAGA GCATGGCATG 
CTTTCAGCAA CTGCTTCCTT GGGACTCATT CTATTATGGG ATGTTGATGG TGGCTTAACT 
ATGATTGACA AATATCTGTA TTCTACCGAT GATTACATCA AATCCGGTGC TTTACTAGCC 
TGTGGTATTG TTAATTGTGG CATACGTAAT GAGGTCGACC CGGCGCATGC GCTACTCTCC 
GATTATATTG ACAACCAGAA TACTTCAATG CGTATCGGCG CCATTTTGGG TCTGGGTATA 
GCATATGCCG GCTCTAATCG TTCCATTGTC ATAGACACAC TAAAAACAGT ATTTGTGTCT 
AATGGTAAAA ATGGATCCAA TGTGGAAATC ATGGGTATAG CTGCACTATC GTTGGGCTTA 
ATATCAGTGG GTTCCTGCAA TGCAGAAATC ACGGAAATCT TACTACAAAC CATCATGGGT 
CTCTCTAAGT CTGATCTCAA AGACACCTAC TCACGTTTCT TGTGGTTAGG CTTGGGGCTA 
TTGTATTTGG GACGCCAGAA AGCCACCGAA GCCGTTATGC TCACCTTAGA GGTACTTGAT 
GAACCATATC GTTCAATGGC CACAACTATG GTGGACATTT GTGCCTACGC AGGCACTGGA 
AATGTTTTGA AAATACAGCA GTTGTTGCAT ATTTGCTCCG ATCATTACGA ATCTGCTAAC 
GGTGATGGTG CCGATGAAAA GGGCAAGAAG GATAAAAATA AGGAAAAGGA TAAGGCCGAA 
AAAGAGAAGG AAAAGGAGAA AGATCTATCC GCAACGCAAG CTATCGCTGT ATTAGGTATT 
GCATTAATTG CTATGGGTGA AGACATTGGT GCCGAGATGG CTTTCCGCTC ATTCGGTAAT 
TTACTACGCT ATTGCGAGCC CTGTGTTCGT CGTGCAGTGC CCCTAGCTCT GGGTCTAATC 
TCTGCGTCCA ATCCAAAATT GAATATTTTG GATACGTTAA GTAAATTCTC GCACGACAGT 
GATGCCGAAG TTGCACATAA TGCCATTTTT GCAATGGGTC TTATCGGTGC TGGCACGAAC 
AATGCACGTT TAGCATCTAT GTTGCGTCAA TTGGCCCAAT ATCATTCAAA GGATCCCAGC 
AATTTGTTTA TGGTACGAAT TGCACAAAGT TTAACACATT TGGGTAAAGG CACATTGACA 
CTCAGTCCTT ACCATAGTGA TCGGCAGTTG ATGAATCCAA TGGCTGTCGC CGGTCTGATG 
GCGACTTTAG TGTCTTTGCT AGATGTTAAA ACCTTAATAT TGGGTCGCTC ACACTACCTA 
CTGTACACGT TAGTGCCAGC CATGCAGGCA CGCATGCTAA TCACTTTTGA TGAAGAACTG 
AATCAACTGC AAGTGCCGGT GCGTGTCGGC ATTGCCATTG ATGTGGTGGG ACAAGCTGGT 
AAACCTAAAA CCATTACCGG TTTCCAAACT CATACAACAC CTGTACTGCT CGCGATGGGT 
GAACGCGCTG AATTGGCCAC AGATGAGTAC ATTTCGCTTA CACCCGTAAT GGAAGGATTT 
GTCATATTGA AGAAAAACCC AAACTTTGTG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011190398.1
CDS	157..2892
Translation	MVAPKKDEKTSAEVKKDQVKETPEKEANKDAKDEGKEQELSDEDQQLQDELEMLVNRLQEADKALYLPALEMLAKLIRASTTSMTSVPKPLKFMRPHYETMKTIYKQMPDQETRQLCADIISVLSMTMGSGKDCLAYRFLCDRSQKIGDWGHEYVRHLSGEIASHYLDTSGEFQAQLIELVKQIIPYNMEHNAEADACDLLIEIDHLHLLQDYVDESAYPRVCLYLQSCYPYVPEPDNTIILETALQLARKFNQHTQALRLALMLNDMDKISEIFKEPKDAAVQKQLAFMLARQQVCLELDESVPDYDDLMEIMSNTNLNKHFLNLARELDIMEAKTPEDIYKSHLDNARTRFASIQVDSAKQNLAASFVNGFVNAGFGVDKLLSEDGNKWLYKNKEHGMLSATASLGLILLWDVDGGLTMIDKYLYSTDDYIKSGALLACGIVNCGIRNEVDPAHALLSDYIDNQNTSMRIGAILGLGIAYAGSNRSIVIDTLKTVFVSNGKNGSNVEIMGIAALSLGLISVGSCNAEITEILLQTIMGLSKSDLKDTYSRFLWLGLGLLYLGRQKATEAVMLTLEVLDEPYRSMATTMVDICAYAGTGNVLKIQQLLHICSDHYESANGDGADEKGKKDKNKEKDKAEKEKEKEKDLSATQAIAVLGIALIAMGEDIGAEMAFRSFGNLLRYCEPCVRRAVPLALGLISASNPKLNILDTLSKFSHDSDAEVAHNAIFAMGLIGAGTNNARLASMLRQLAQYHSKDPSNLFMVRIAQSLTHLGKGTLTLSPYHSDRQLMNPMAVAGLMATLVSLLDVKTLILGRSHYLLYTLVPAMQARMLITFDEELNQLQVPVRVGIAIDVVGQAGKPKTITGFQTHTTPVLLAMGERAELATDEYISLTPVMEGFVILKKNPNFVK

Target ORF information:

RefSeq Version	XM_011192096.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae 26S proteasome non-ATPase regulatory subunit 2 (LOC105217199), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011192096.1

ORF Insert Sequence:

ATGGTTGCTC CCAAGAAAGA TGAAAAAACT TCCGCTGAGG TGAAGAAAGA CCAAGTGAAG 
GAGACACCGG AAAAAGAAGC AAATAAAGAT GCTAAAGACG AAGGTAAGGA GCAGGAGCTT 
TCCGATGAGG ATCAACAGCT GCAAGATGAA CTTGAAATGC TGGTTAATCG CCTACAAGAG 
GCCGATAAGG CATTGTATTT ACCAGCATTG GAAATGTTGG CAAAACTTAT ACGTGCATCT 
ACTACATCTA TGACTTCTGT ACCGAAACCA TTGAAGTTTA TGCGTCCCCA TTATGAGACT 
ATGAAAACAA TATACAAACA AATGCCTGAT CAGGAAACTC GGCAATTGTG CGCTGACATC 
ATATCCGTGC TGTCAATGAC AATGGGAAGT GGTAAGGATT GTTTGGCTTA CCGTTTTCTT 
TGTGATCGTT CGCAGAAAAT CGGTGATTGG GGTCACGAAT ATGTGCGCCA TTTGTCTGGA 
GAAATAGCTT CGCACTATTT GGATACATCA GGAGAATTTC AGGCACAGCT AATCGAGTTG 
GTGAAACAAA TAATCCCATA CAATATGGAA CACAATGCCG AGGCTGATGC TTGCGATTTA 
TTAATTGAGA TCGATCATTT GCATTTGTTG CAAGATTATG TCGATGAATC CGCTTATCCA 
CGCGTCTGTT TATACTTACA ATCTTGCTAT CCATATGTAC CCGAACCGGA TAATACTATA 
ATTCTGGAAA CAGCGCTACA GTTGGCACGT AAATTTAATC AACATACACA AGCGCTGCGT 
CTAGCATTGA TGTTAAATGA TATGGATAAA ATTTCCGAAA TATTCAAGGA ACCTAAAGAT 
GCAGCTGTGC AAAAACAGCT TGCTTTTATG TTAGCACGTC AGCAAGTATG CTTGGAATTA 
GATGAGTCGG TGCCGGATTA TGATGATTTA ATGGAAATCA TGTCTAACAC GAACTTGAAT 
AAACATTTTT TGAATTTGGC ACGTGAACTT GATATAATGG AAGCCAAGAC ACCAGAAGAC 
ATTTACAAAT CGCATTTGGA TAATGCGCGC ACTCGTTTCG CTTCAATACA GGTTGATTCG 
GCGAAACAGA ATTTGGCTGC CAGTTTTGTG AATGGTTTTG TTAACGCTGG CTTTGGTGTG 
GACAAACTAC TCTCTGAAGA TGGCAATAAG TGGTTGTATA AAAATAAAGA GCATGGCATG 
CTTTCAGCAA CTGCTTCCTT GGGACTCATT CTATTATGGG ATGTTGATGG TGGCTTAACT 
ATGATTGACA AATATCTGTA TTCTACCGAT GATTACATCA AATCCGGTGC TTTACTAGCC 
TGTGGTATTG TTAATTGTGG CATACGTAAT GAGGTCGACC CGGCGCATGC GCTACTCTCC 
GATTATATTG ACAACCAGAA TACTTCAATG CGTATCGGCG CCATTTTGGG TCTGGGTATA 
GCATATGCCG GCTCTAATCG TTCCATTGTC ATAGACACAC TAAAAACAGT ATTTGTGTCT 
AATGGTAAAA ATGGATCCAA TGTGGAAATC ATGGGTATAG CTGCACTATC GTTGGGCTTA 
ATATCAGTGG GTTCCTGCAA TGCAGAAATC ACGGAAATCT TACTACAAAC CATCATGGGT 
CTCTCTAAGT CTGATCTCAA AGACACCTAC TCACGTTTCT TGTGGTTAGG CTTGGGGCTA 
TTGTATTTGG GACGCCAGAA AGCCACCGAA GCCGTTATGC TCACCTTAGA GGTACTTGAT 
GAACCATATC GTTCAATGGC CACAACTATG GTGGACATTT GTGCCTACGC AGGCACTGGA 
AATGTTTTGA AAATACAGCA GTTGTTGCAT ATTTGCTCCG ATCATTACGA ATCTGCTAAC 
GGTGATGGTG CCGATGAAAA GGGCAAGAAG GATAAAAATA AGGAAAAGGA TAAGGCCGAA 
AAAGAGAAGG AAAAGGAGAA AGATCTATCC GCAACGCAAG CTATCGCTGT ATTAGGTATT 
GCATTAATTG CTATGGGTGA AGACATTGGT GCCGAGATGG CTTTCCGCTC ATTCGGTAAT 
TTACTACGCT ATTGCGAGCC CTGTGTTCGT CGTGCAGTGC CCCTAGCTCT GGGTCTAATC 
TCTGCGTCCA ATCCAAAATT GAATATTTTG GATACGTTAA GTAAATTCTC GCACGACAGT 
GATGCCGAAG TTGCACATAA TGCCATTTTT GCAATGGGTC TTATCGGTGC TGGCACGAAC 
AATGCACGTT TAGCATCTAT GTTGCGTCAA TTGGCCCAAT ATCATTCAAA GGATCCCAGC 
AATTTGTTTA TGGTACGAAT TGCACAAAGT TTAACACATT TGGGTAAAGG CACATTGACA 
CTCAGTCCTT ACCATAGTGA TCGGCAGTTG ATGAATCCAA TGGCTGTCGC CGGTCTGATG 
GCGACTTTAG TGTCTTTGCT AGATGTTAAA ACCTTAATAT TGGGTCGCTC ACACTACCTA 
CTGTACACGT TAGTGCCAGC CATGCAGGCA CGCATGCTAA TCACTTTTGA TGAAGAACTG 
AATCAACTGC AAGTGCCGGT GCGTGTCGGC ATTGCCATTG ATGTGGTGGG ACAAGCTGGT 
AAACCTAAAA CCATTACCGG TTTCCAAACT CATACAACAC CTGTACTGCT CGCGATGGGT 
GAACGCGCTG AATTGGCCAC AGATGAGTAC ATTTCGCTTA CACCCGTAAT GGAAGGATTT 
GTCATATTGA AGAAAAACCC AAACTTTGTG AAGTAA