LOC105208666 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105208666
Entrez Gene ID	105208666
Full Name	protein MMS22-like
Synonyms	CG14803_1
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011178646.1	XP_011176948.1	protein MMS22-like
XM_011178646.2	XP_011176948.1	protein MMS22-like

Pathways from BioSystems
Gene Ontology

The following LOC105208666 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105208666 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa194387	XM_011178646.2 Latest version!	Zeugodacus cucurbitae protein MMS22-like (LOC105208666), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OBa194387	XM_011178646.1	Bactrocera cucurbitae protein MMS22-like (LOC105208666), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105208666 ( XM_011178646.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176948.1 Bactrocera cucurbitae protein MMS22-like (LOC105208666), mRNA.

Price & Availability↑

CloneID	OBa194387
Clone ID Related Accession (Same CDS sequence)	XM_011178646.1 , XM_011178646.2
Accession Version	XM_011178646.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	protein MMS22-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863674.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTG AATTGTTTAA TTTGGATGAA GACGATGATT TATTAAGCAC AGCATGCATA 
GAAGCAACTC AAGATCATCT AACGGCACCG CAGCAAACAC AAGCATTTGT TGGTGTCTGT 
GATGAAACGG TCCTGCCTGA ACTGCAATGT AACGGTGAGG ATACGACACA TGCTCAATTA 
GCAAGTGATG GATTTGTGAA AAGTGGATTT ATGTTGACGC GACCAGAATA TTACTGGCGC 
GATGCAAGTG AAGCGCTAAC ACTTACAGAA CTTGAATTTG GTGTAAATCA GCTGTATGGA 
CACAATAATG TGCAATATCT CATTGGCACA GCTTGCATGC ATATCCAACA TCTGTGGGCT 
AGCGTACGAC AGCCACTTAA CACAAATGAC GCATATAACT ACCACAAACG CAGGAAAGAA 
GTGACGCATT TGTTTTATTG GATACTGCAG CACACAACCG ACGAACAGTG GCCATTGTTG 
CGCCCCAGCT TGGAGAGCAT ACGTCGTCTA ATTAATCGTA CAACTGAAGC AAACACCTGG 
AAGTTAATAT ATTTTGCAAA TAGTTGTAAA GGAAATGATT GCAATGCTGC CGCCTACCAC 
ATGTTGCATG GTGTGCTCGA GTGGAAGCTG TTGGATTTGT GTATACTTTA CAGATTCGAA 
ATTTGTGCGC CTACAACAAT GTTGGATAGC AATACAAAGC CCAACAGCGC CTTCTTGAGT 
CAATTGACAC TATACTTGGA GGATTTAGTG ACGTGCTCAT TGTATTTGTA TGTGAAAAAG 
CGACCAGTTG AGTTGCTATA TGCTTCGCCC TTTCCATGTA CATGCATAAA GGAGTGCTGG 
TTGTATATAC AACTCGCATT GCAGAAGTGG TCATTGACTG CACTTGCGGT GGGTGGTGAG 
CAACTTACCT TTTGGCAACT ATTTAATGAA ACAATGGCAA AATTAAAAAC CAAATTGGAT 
ACATTTGCTT TAACCACATT ATCATTGGCT GAATTCTCAA ATTGGCTGCT ACATGCGCTC 
GTGCGGCTGT TTGGTTATCG CTCTAATGGG CAGTTTGTAG GTCCAAGGCA TGAGCGCGCT 
GTGGCTGAAA CGGCGGAGAA TTTGGACGCG CTGGAGCAAT GTACACAACA GTTCCTCAAC 
GGTAATCCCA GCGAAGAGCA GACACGCGTT TATATTTGCC TAATAATGCC TACGCTGTTG 
CATTGGTGGC GTCCACGCGT CACAATACCA ATTCTTTTGT GGGAGCATTT CCACAAGCGT 
CTAAATACTA CATTCTATGC TGCCGGTTCG GCACCAAGTA ATTTGGCTGT CGCTTGTGCC 
AGCGGCAAGG CATATGTGGC GCGTTATCGC GCGCTATTGC AGCAAGAAGA ATTCACACCT 
GATGCCAATT TGAGTAGTTA CACGCTGTTC ACGTTATTGC TGGGCAAGAA TCTAATGCGC 
TTGCTGGAAA ACGCAGCGAC GCGTCATCAG GCGCAAAAAC TGCTCGGCCG CATTTATACC 
AAATTCAGCG CGCAAAAGTT TCTCGCGCTA AATGAAATGG GCATACACCA TCTGATTGAG 
TTATTCCTGG CGCTAGCGCT TTGTGGTGAT TTCGCAGAGC TTGCGCCGAA GCTGAGCGCG 
AAATTGCTAT CCATCGCCTT GGATAAAATA CCTGCTGGAC GTCAAATTGC AGTAACTAAA 
GGTCATATGG CGCTATTGAT ACTCTATGCG GAGCAGCGCT GTGAAATTGG CGAATATGTG 
ACGAAACTGT TGCAACAGCT CGCTGCTATT AATAACGATT TGGGCGTTTC GAAAGTGCTG 
GCAGATGGTT TGTTGGAGAT TTTCATGCAT GCCGACGATT TTCGACGCGG CGAACACTTG 
CTCATAGAGG CGTGGCTGCC GAATTATCTG CAAACCTGCC CGCCGGTGGA ACAAGAACGC 
AGCTTGGAGG CCTTACATCT GATATTCGGC AAATTGCAGC GCAACGAAGC GCTAATCGAG 
TCGAACGCGG CGCTCTTCAA AGCGCTCAAT GCGCATGTGT TGCCGTTTGT CAAGCAACAA 
TTTGCTTGCG GTTACAGCCA ATGGCTGCCG CAGCTCGCCG CAGACTTTTC CACGCACGCC 
TGCACAGTGC CGGACACGCA GACGTTTCAG AAACTATTCA CCTGCTTCAT CGACGCGCCG 
GTTGCATGCA GACAGGCGCT GCCGCAATTT CTCAATAAAG TGTTGCAAAC TGAGCGCGCC 
GCTTTGATTG AGCCGAATGT GGTAATACAT GTGTGGCTGA AGAGTTTGGT GCAGCTGACC 
GCTGTTAACG AGGATGTGGC GCAATTGACG CGCTATGTGT CGCAGTTGGC GGCATTTCGC 
ATGATGACCG ACAATTTGAA TCCCGAAGAG CTGCTGCAGG CAAAAGAACC GCTATGTGTG 
TTCATAGCCG CTGTTGGCAA ACATTACGAC GCATGTGCGT CGGATCATCA ACGGCGTTTT 
CGCATTGCGG ACAATTTGAA TGCATATTTA CGCAATTTCG ACAAATGGCT ACAGTCGGAC 
CTAAAGCAGG AGAAGACCGA AGTGGCCTTT CGCTTCTACA GTTTCCTGGC CATAGTTATA 
TACAATTGCG CGCACATCGT ATACACCAAA TCGAAGGTGA ACTGTTTCTT TCACGTGGTC 
ATGACGCGTT TCGTGCTGCC CACCAATGTG CAGATGGGCA AAGCGCCCGA TGGCAAATTG 
GCGCAAGTGG TGCATAAAAT TTGGCCTGTG CTCGTGCAGG GTATTGGCCG ACTCAATTTC 
AAAGCCGACC CGTATATTAG CAAAACGCTC ACCGACCTCA TACAAAAGTG GACGCCACAT 
TTCAAAATCT CACCGAACGC CAAAGTGGTC GCACGCCCCT TTGTCAGCTG TCTGCAAAGC 
GACAACGCTG AATTGTCGCT ATTTGTCTTC GAGAAGCTAA CCAGCTTGTT CTTGGCCACA 
CAGCGCCGTC AAGCCGATCC GAATGCCTGC CTAGTAGTGA CTATCTTCCA AGAAGTGATC 
GAGTCCATAG ACGCCACCGC CGCCACTAAC GCCAGTGACG ACGTGTTAAC GCAACGTTTG 
GAGACTTTCA TGAAAGCCAC ATCGCTGCTG ATGTTGGAGC ACATTATGAT GGTGGATGAG 
GTGGTGCCCA GTCGCGCGCT TTTACTGGAT CTGTTTAAAC GCATCGTCAG CAGCGCGACA 
TATAAGCGCA CAGCCGCTTT ACAACAACTA CTGGCCGAGC ACTTGCGTAT ACTTACCAAA 
AAGCATCTCG CCTACTACAC TTTCTTCTTC TTCGAGCTGT TGCAGCGTTT GGCGCAATTC 
ACACCGGACA CGATTTTGCA AATCATGGAT TTTCTGCTCG CCGAGTTGCG TGTTGTGGAG 
CAGAAGCGTG GCTCCGGTGA GGATAATCGC ATGAGAGGAT GCTTGCAAAA GTTACAACAG 
ACTTTGAAAT GTGACAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176948.1
CDS	140..3580
Translation	MDFELFNLDEDDDLLSTACIEATQDHLTAPQQTQAFVGVCDETVLPELQCNGEDTTHAQLASDGFVKSGFMLTRPEYYWRDASEALTLTELEFGVNQLYGHNNVQYLIGTACMHIQHLWASVRQPLNTNDAYNYHKRRKEVTHLFYWILQHTTDEQWPLLRPSLESIRRLINRTTEANTWKLIYFANSCKGNDCNAAAYHMLHGVLEWKLLDLCILYRFEICAPTTMLDSNTKPNSAFLSQLTLYLEDLVTCSLYLYVKKRPVELLYASPFPCTCIKECWLYIQLALQKWSLTALAVGGEQLTFWQLFNETMAKLKTKLDTFALTTLSLAEFSNWLLHALVRLFGYRSNGQFVGPRHERAVAETAENLDALEQCTQQFLNGNPSEEQTRVYICLIMPTLLHWWRPRVTIPILLWEHFHKRLNTTFYAAGSAPSNLAVACASGKAYVARYRALLQQEEFTPDANLSSYTLFTLLLGKNLMRLLENAATRHQAQKLLGRIYTKFSAQKFLALNEMGIHHLIELFLALALCGDFAELAPKLSAKLLSIALDKIPAGRQIAVTKGHMALLILYAEQRCEIGEYVTKLLQQLAAINNDLGVSKVLADGLLEIFMHADDFRRGEHLLIEAWLPNYLQTCPPVEQERSLEALHLIFGKLQRNEALIESNAALFKALNAHVLPFVKQQFACGYSQWLPQLAADFSTHACTVPDTQTFQKLFTCFIDAPVACRQALPQFLNKVLQTERAALIEPNVVIHVWLKSLVQLTAVNEDVAQLTRYVSQLAAFRMMTDNLNPEELLQAKEPLCVFIAAVGKHYDACASDHQRRFRIADNLNAYLRNFDKWLQSDLKQEKTEVAFRFYSFLAIVIYNCAHIVYTKSKVNCFFHVVMTRFVLPTNVQMGKAPDGKLAQVVHKIWPVLVQGIGRLNFKADPYISKTLTDLIQKWTPHFKISPNAKVVARPFVSCLQSDNAELSLFVFEKLTSLFLATQRRQADPNACLVVTIFQEVIESIDATAATNASDDVLTQRLETFMKATSLLMLEHIMMVDEVVPSRALLLDLFKRIVSSATYKRTAALQQLLAEHLRILTKKHLAYYTFFFFELLQRLAQFTPDTILQIMDFLLAELRVVEQKRGSGEDNRMRGCLQKLQQTLKCDR

Target ORF information:

RefSeq Version	XM_011178646.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae protein MMS22-like (LOC105208666), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178646.1

ORF Insert Sequence:

ATGGATTTTG AATTGTTTAA TTTGGATGAA GACGATGATT TATTAAGCAC AGCATGCATA 
GAAGCAACTC AAGATCATCT AACGGCACCG CAGCAAACAC AAGCATTTGT TGGTGTCTGT 
GATGAAACGG TCCTGCCTGA ACTGCAATGT AACGGTGAGG ATACGACACA TGCTCAATTA 
GCAAGTGATG GATTTGTGAA AAGTGGATTT ATGTTGACGC GACCAGAATA TTACTGGCGC 
GATGCAAGTG AAGCGCTAAC ACTTACAGAA CTTGAATTTG GTGTAAATCA GCTGTATGGA 
CACAATAATG TGCAATATCT CATTGGCACA GCTTGCATGC ATATCCAACA TCTGTGGGCT 
AGCGTACGAC AGCCACTTAA CACAAATGAC GCATATAACT ACCACAAACG CAGGAAAGAA 
GTGACGCATT TGTTTTATTG GATACTGCAG CACACAACCG ACGAACAGTG GCCATTGTTG 
CGCCCCAGCT TGGAGAGCAT ACGTCGTCTA ATTAATCGTA CAACTGAAGC AAACACCTGG 
AAGTTAATAT ATTTTGCAAA TAGTTGTAAA GGAAATGATT GCAATGCTGC CGCCTACCAC 
ATGTTGCATG GTGTGCTCGA GTGGAAGCTG TTGGATTTGT GTATACTTTA CAGATTCGAA 
ATTTGTGCGC CTACAACAAT GTTGGATAGC AATACAAAGC CCAACAGCGC CTTCTTGAGT 
CAATTGACAC TATACTTGGA GGATTTAGTG ACGTGCTCAT TGTATTTGTA TGTGAAAAAG 
CGACCAGTTG AGTTGCTATA TGCTTCGCCC TTTCCATGTA CATGCATAAA GGAGTGCTGG 
TTGTATATAC AACTCGCATT GCAGAAGTGG TCATTGACTG CACTTGCGGT GGGTGGTGAG 
CAACTTACCT TTTGGCAACT ATTTAATGAA ACAATGGCAA AATTAAAAAC CAAATTGGAT 
ACATTTGCTT TAACCACATT ATCATTGGCT GAATTCTCAA ATTGGCTGCT ACATGCGCTC 
GTGCGGCTGT TTGGTTATCG CTCTAATGGG CAGTTTGTAG GTCCAAGGCA TGAGCGCGCT 
GTGGCTGAAA CGGCGGAGAA TTTGGACGCG CTGGAGCAAT GTACACAACA GTTCCTCAAC 
GGTAATCCCA GCGAAGAGCA GACACGCGTT TATATTTGCC TAATAATGCC TACGCTGTTG 
CATTGGTGGC GTCCACGCGT CACAATACCA ATTCTTTTGT GGGAGCATTT CCACAAGCGT 
CTAAATACTA CATTCTATGC TGCCGGTTCG GCACCAAGTA ATTTGGCTGT CGCTTGTGCC 
AGCGGCAAGG CATATGTGGC GCGTTATCGC GCGCTATTGC AGCAAGAAGA ATTCACACCT 
GATGCCAATT TGAGTAGTTA CACGCTGTTC ACGTTATTGC TGGGCAAGAA TCTAATGCGC 
TTGCTGGAAA ACGCAGCGAC GCGTCATCAG GCGCAAAAAC TGCTCGGCCG CATTTATACC 
AAATTCAGCG CGCAAAAGTT TCTCGCGCTA AATGAAATGG GCATACACCA TCTGATTGAG 
TTATTCCTGG CGCTAGCGCT TTGTGGTGAT TTCGCAGAGC TTGCGCCGAA GCTGAGCGCG 
AAATTGCTAT CCATCGCCTT GGATAAAATA CCTGCTGGAC GTCAAATTGC AGTAACTAAA 
GGTCATATGG CGCTATTGAT ACTCTATGCG GAGCAGCGCT GTGAAATTGG CGAATATGTG 
ACGAAACTGT TGCAACAGCT CGCTGCTATT AATAACGATT TGGGCGTTTC GAAAGTGCTG 
GCAGATGGTT TGTTGGAGAT TTTCATGCAT GCCGACGATT TTCGACGCGG CGAACACTTG 
CTCATAGAGG CGTGGCTGCC GAATTATCTG CAAACCTGCC CGCCGGTGGA ACAAGAACGC 
AGCTTGGAGG CCTTACATCT GATATTCGGC AAATTGCAGC GCAACGAAGC GCTAATCGAG 
TCGAACGCGG CGCTCTTCAA AGCGCTCAAT GCGCATGTGT TGCCGTTTGT CAAGCAACAA 
TTTGCTTGCG GTTACAGCCA ATGGCTGCCG CAGCTCGCCG CAGACTTTTC CACGCACGCC 
TGCACAGTGC CGGACACGCA GACGTTTCAG AAACTATTCA CCTGCTTCAT CGACGCGCCG 
GTTGCATGCA GACAGGCGCT GCCGCAATTT CTCAATAAAG TGTTGCAAAC TGAGCGCGCC 
GCTTTGATTG AGCCGAATGT GGTAATACAT GTGTGGCTGA AGAGTTTGGT GCAGCTGACC 
GCTGTTAACG AGGATGTGGC GCAATTGACG CGCTATGTGT CGCAGTTGGC GGCATTTCGC 
ATGATGACCG ACAATTTGAA TCCCGAAGAG CTGCTGCAGG CAAAAGAACC GCTATGTGTG 
TTCATAGCCG CTGTTGGCAA ACATTACGAC GCATGTGCGT CGGATCATCA ACGGCGTTTT 
CGCATTGCGG ACAATTTGAA TGCATATTTA CGCAATTTCG ACAAATGGCT ACAGTCGGAC 
CTAAAGCAGG AGAAGACCGA AGTGGCCTTT CGCTTCTACA GTTTCCTGGC CATAGTTATA 
TACAATTGCG CGCACATCGT ATACACCAAA TCGAAGGTGA ACTGTTTCTT TCACGTGGTC 
ATGACGCGTT TCGTGCTGCC CACCAATGTG CAGATGGGCA AAGCGCCCGA TGGCAAATTG 
GCGCAAGTGG TGCATAAAAT TTGGCCTGTG CTCGTGCAGG GTATTGGCCG ACTCAATTTC 
AAAGCCGACC CGTATATTAG CAAAACGCTC ACCGACCTCA TACAAAAGTG GACGCCACAT 
TTCAAAATCT CACCGAACGC CAAAGTGGTC GCACGCCCCT TTGTCAGCTG TCTGCAAAGC 
GACAACGCTG AATTGTCGCT ATTTGTCTTC GAGAAGCTAA CCAGCTTGTT CTTGGCCACA 
CAGCGCCGTC AAGCCGATCC GAATGCCTGC CTAGTAGTGA CTATCTTCCA AGAAGTGATC 
GAGTCCATAG ACGCCACCGC CGCCACTAAC GCCAGTGACG ACGTGTTAAC GCAACGTTTG 
GAGACTTTCA TGAAAGCCAC ATCGCTGCTG ATGTTGGAGC ACATTATGAT GGTGGATGAG 
GTGGTGCCCA GTCGCGCGCT TTTACTGGAT CTGTTTAAAC GCATCGTCAG CAGCGCGACA 
TATAAGCGCA CAGCCGCTTT ACAACAACTA CTGGCCGAGC ACTTGCGTAT ACTTACCAAA 
AAGCATCTCG CCTACTACAC TTTCTTCTTC TTCGAGCTGT TGCAGCGTTT GGCGCAATTC 
ACACCGGACA CGATTTTGCA AATCATGGAT TTTCTGCTCG CCGAGTTGCG TGTTGTGGAG 
CAGAAGCGTG GCTCCGGTGA GGATAATCGC ATGAGAGGAT GCTTGCAAAA GTTACAACAG 
ACTTTGAAAT GTGACAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208666 ( XM_011178646.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176948.1 Zeugodacus cucurbitae protein MMS22-like (LOC105208666), mRNA.

Price & Availability↑

CloneID	OBa194387
Clone ID Related Accession (Same CDS sequence)	XM_011178646.1 , XM_011178646.2
Accession Version	XM_011178646.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	protein MMS22-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863674.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011178646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTTG AATTGTTTAA TTTGGATGAA GACGATGATT TATTAAGCAC AGCATGCATA 
GAAGCAACTC AAGATCATCT AACGGCACCG CAGCAAACAC AAGCATTTGT TGGTGTCTGT 
GATGAAACGG TCCTGCCTGA ACTGCAATGT AACGGTGAGG ATACGACACA TGCTCAATTA 
GCAAGTGATG GATTTGTGAA AAGTGGATTT ATGTTGACGC GACCAGAATA TTACTGGCGC 
GATGCAAGTG AAGCGCTAAC ACTTACAGAA CTTGAATTTG GTGTAAATCA GCTGTATGGA 
CACAATAATG TGCAATATCT CATTGGCACA GCTTGCATGC ATATCCAACA TCTGTGGGCT 
AGCGTACGAC AGCCACTTAA CACAAATGAC GCATATAACT ACCACAAACG CAGGAAAGAA 
GTGACGCATT TGTTTTATTG GATACTGCAG CACACAACCG ACGAACAGTG GCCATTGTTG 
CGCCCCAGCT TGGAGAGCAT ACGTCGTCTA ATTAATCGTA CAACTGAAGC AAACACCTGG 
AAGTTAATAT ATTTTGCAAA TAGTTGTAAA GGAAATGATT GCAATGCTGC CGCCTACCAC 
ATGTTGCATG GTGTGCTCGA GTGGAAGCTG TTGGATTTGT GTATACTTTA CAGATTCGAA 
ATTTGTGCGC CTACAACAAT GTTGGATAGC AATACAAAGC CCAACAGCGC CTTCTTGAGT 
CAATTGACAC TATACTTGGA GGATTTAGTG ACGTGCTCAT TGTATTTGTA TGTGAAAAAG 
CGACCAGTTG AGTTGCTATA TGCTTCGCCC TTTCCATGTA CATGCATAAA GGAGTGCTGG 
TTGTATATAC AACTCGCATT GCAGAAGTGG TCATTGACTG CACTTGCGGT GGGTGGTGAG 
CAACTTACCT TTTGGCAACT ATTTAATGAA ACAATGGCAA AATTAAAAAC CAAATTGGAT 
ACATTTGCTT TAACCACATT ATCATTGGCT GAATTCTCAA ATTGGCTGCT ACATGCGCTC 
GTGCGGCTGT TTGGTTATCG CTCTAATGGG CAGTTTGTAG GTCCAAGGCA TGAGCGCGCT 
GTGGCTGAAA CGGCGGAGAA TTTGGACGCG CTGGAGCAAT GTACACAACA GTTCCTCAAC 
GGTAATCCCA GCGAAGAGCA GACACGCGTT TATATTTGCC TAATAATGCC TACGCTGTTG 
CATTGGTGGC GTCCACGCGT CACAATACCA ATTCTTTTGT GGGAGCATTT CCACAAGCGT 
CTAAATACTA CATTCTATGC TGCCGGTTCG GCACCAAGTA ATTTGGCTGT CGCTTGTGCC 
AGCGGCAAGG CATATGTGGC GCGTTATCGC GCGCTATTGC AGCAAGAAGA ATTCACACCT 
GATGCCAATT TGAGTAGTTA CACGCTGTTC ACGTTATTGC TGGGCAAGAA TCTAATGCGC 
TTGCTGGAAA ACGCAGCGAC GCGTCATCAG GCGCAAAAAC TGCTCGGCCG CATTTATACC 
AAATTCAGCG CGCAAAAGTT TCTCGCGCTA AATGAAATGG GCATACACCA TCTGATTGAG 
TTATTCCTGG CGCTAGCGCT TTGTGGTGAT TTCGCAGAGC TTGCGCCGAA GCTGAGCGCG 
AAATTGCTAT CCATCGCCTT GGATAAAATA CCTGCTGGAC GTCAAATTGC AGTAACTAAA 
GGTCATATGG CGCTATTGAT ACTCTATGCG GAGCAGCGCT GTGAAATTGG CGAATATGTG 
ACGAAACTGT TGCAACAGCT CGCTGCTATT AATAACGATT TGGGCGTTTC GAAAGTGCTG 
GCAGATGGTT TGTTGGAGAT TTTCATGCAT GCCGACGATT TTCGACGCGG CGAACACTTG 
CTCATAGAGG CGTGGCTGCC GAATTATCTG CAAACCTGCC CGCCGGTGGA ACAAGAACGC 
AGCTTGGAGG CCTTACATCT GATATTCGGC AAATTGCAGC GCAACGAAGC GCTAATCGAG 
TCGAACGCGG CGCTCTTCAA AGCGCTCAAT GCGCATGTGT TGCCGTTTGT CAAGCAACAA 
TTTGCTTGCG GTTACAGCCA ATGGCTGCCG CAGCTCGCCG CAGACTTTTC CACGCACGCC 
TGCACAGTGC CGGACACGCA GACGTTTCAG AAACTATTCA CCTGCTTCAT CGACGCGCCG 
GTTGCATGCA GACAGGCGCT GCCGCAATTT CTCAATAAAG TGTTGCAAAC TGAGCGCGCC 
GCTTTGATTG AGCCGAATGT GGTAATACAT GTGTGGCTGA AGAGTTTGGT GCAGCTGACC 
GCTGTTAACG AGGATGTGGC GCAATTGACG CGCTATGTGT CGCAGTTGGC GGCATTTCGC 
ATGATGACCG ACAATTTGAA TCCCGAAGAG CTGCTGCAGG CAAAAGAACC GCTATGTGTG 
TTCATAGCCG CTGTTGGCAA ACATTACGAC GCATGTGCGT CGGATCATCA ACGGCGTTTT 
CGCATTGCGG ACAATTTGAA TGCATATTTA CGCAATTTCG ACAAATGGCT ACAGTCGGAC 
CTAAAGCAGG AGAAGACCGA AGTGGCCTTT CGCTTCTACA GTTTCCTGGC CATAGTTATA 
TACAATTGCG CGCACATCGT ATACACCAAA TCGAAGGTGA ACTGTTTCTT TCACGTGGTC 
ATGACGCGTT TCGTGCTGCC CACCAATGTG CAGATGGGCA AAGCGCCCGA TGGCAAATTG 
GCGCAAGTGG TGCATAAAAT TTGGCCTGTG CTCGTGCAGG GTATTGGCCG ACTCAATTTC 
AAAGCCGACC CGTATATTAG CAAAACGCTC ACCGACCTCA TACAAAAGTG GACGCCACAT 
TTCAAAATCT CACCGAACGC CAAAGTGGTC GCACGCCCCT TTGTCAGCTG TCTGCAAAGC 
GACAACGCTG AATTGTCGCT ATTTGTCTTC GAGAAGCTAA CCAGCTTGTT CTTGGCCACA 
CAGCGCCGTC AAGCCGATCC GAATGCCTGC CTAGTAGTGA CTATCTTCCA AGAAGTGATC 
GAGTCCATAG ACGCCACCGC CGCCACTAAC GCCAGTGACG ACGTGTTAAC GCAACGTTTG 
GAGACTTTCA TGAAAGCCAC ATCGCTGCTG ATGTTGGAGC ACATTATGAT GGTGGATGAG 
GTGGTGCCCA GTCGCGCGCT TTTACTGGAT CTGTTTAAAC GCATCGTCAG CAGCGCGACA 
TATAAGCGCA CAGCCGCTTT ACAACAACTA CTGGCCGAGC ACTTGCGTAT ACTTACCAAA 
AAGCATCTCG CCTACTACAC TTTCTTCTTC TTCGAGCTGT TGCAGCGTTT GGCGCAATTC 
ACACCGGACA CGATTTTGCA AATCATGGAT TTTCTGCTCG CCGAGTTGCG TGTTGTGGAG 
CAGAAGCGTG GCTCCGGTGA GGATAATCGC ATGAGAGGAT GCTTGCAAAA GTTACAACAG 
ACTTTGAAAT GTGACAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176948.1
CDS	138..3578
Translation	MDFELFNLDEDDDLLSTACIEATQDHLTAPQQTQAFVGVCDETVLPELQCNGEDTTHAQLASDGFVKSGFMLTRPEYYWRDASEALTLTELEFGVNQLYGHNNVQYLIGTACMHIQHLWASVRQPLNTNDAYNYHKRRKEVTHLFYWILQHTTDEQWPLLRPSLESIRRLINRTTEANTWKLIYFANSCKGNDCNAAAYHMLHGVLEWKLLDLCILYRFEICAPTTMLDSNTKPNSAFLSQLTLYLEDLVTCSLYLYVKKRPVELLYASPFPCTCIKECWLYIQLALQKWSLTALAVGGEQLTFWQLFNETMAKLKTKLDTFALTTLSLAEFSNWLLHALVRLFGYRSNGQFVGPRHERAVAETAENLDALEQCTQQFLNGNPSEEQTRVYICLIMPTLLHWWRPRVTIPILLWEHFHKRLNTTFYAAGSAPSNLAVACASGKAYVARYRALLQQEEFTPDANLSSYTLFTLLLGKNLMRLLENAATRHQAQKLLGRIYTKFSAQKFLALNEMGIHHLIELFLALALCGDFAELAPKLSAKLLSIALDKIPAGRQIAVTKGHMALLILYAEQRCEIGEYVTKLLQQLAAINNDLGVSKVLADGLLEIFMHADDFRRGEHLLIEAWLPNYLQTCPPVEQERSLEALHLIFGKLQRNEALIESNAALFKALNAHVLPFVKQQFACGYSQWLPQLAADFSTHACTVPDTQTFQKLFTCFIDAPVACRQALPQFLNKVLQTERAALIEPNVVIHVWLKSLVQLTAVNEDVAQLTRYVSQLAAFRMMTDNLNPEELLQAKEPLCVFIAAVGKHYDACASDHQRRFRIADNLNAYLRNFDKWLQSDLKQEKTEVAFRFYSFLAIVIYNCAHIVYTKSKVNCFFHVVMTRFVLPTNVQMGKAPDGKLAQVVHKIWPVLVQGIGRLNFKADPYISKTLTDLIQKWTPHFKISPNAKVVARPFVSCLQSDNAELSLFVFEKLTSLFLATQRRQADPNACLVVTIFQEVIESIDATAATNASDDVLTQRLETFMKATSLLMLEHIMMVDEVVPSRALLLDLFKRIVSSATYKRTAALQQLLAEHLRILTKKHLAYYTFFFFELLQRLAQFTPDTILQIMDFLLAELRVVEQKRGSGEDNRMRGCLQKLQQTLKCDR

Target ORF information:

RefSeq Version	XM_011178646.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae protein MMS22-like (LOC105208666), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178646.2

ORF Insert Sequence:

ATGGATTTTG AATTGTTTAA TTTGGATGAA GACGATGATT TATTAAGCAC AGCATGCATA 
GAAGCAACTC AAGATCATCT AACGGCACCG CAGCAAACAC AAGCATTTGT TGGTGTCTGT 
GATGAAACGG TCCTGCCTGA ACTGCAATGT AACGGTGAGG ATACGACACA TGCTCAATTA 
GCAAGTGATG GATTTGTGAA AAGTGGATTT ATGTTGACGC GACCAGAATA TTACTGGCGC 
GATGCAAGTG AAGCGCTAAC ACTTACAGAA CTTGAATTTG GTGTAAATCA GCTGTATGGA 
CACAATAATG TGCAATATCT CATTGGCACA GCTTGCATGC ATATCCAACA TCTGTGGGCT 
AGCGTACGAC AGCCACTTAA CACAAATGAC GCATATAACT ACCACAAACG CAGGAAAGAA 
GTGACGCATT TGTTTTATTG GATACTGCAG CACACAACCG ACGAACAGTG GCCATTGTTG 
CGCCCCAGCT TGGAGAGCAT ACGTCGTCTA ATTAATCGTA CAACTGAAGC AAACACCTGG 
AAGTTAATAT ATTTTGCAAA TAGTTGTAAA GGAAATGATT GCAATGCTGC CGCCTACCAC 
ATGTTGCATG GTGTGCTCGA GTGGAAGCTG TTGGATTTGT GTATACTTTA CAGATTCGAA 
ATTTGTGCGC CTACAACAAT GTTGGATAGC AATACAAAGC CCAACAGCGC CTTCTTGAGT 
CAATTGACAC TATACTTGGA GGATTTAGTG ACGTGCTCAT TGTATTTGTA TGTGAAAAAG 
CGACCAGTTG AGTTGCTATA TGCTTCGCCC TTTCCATGTA CATGCATAAA GGAGTGCTGG 
TTGTATATAC AACTCGCATT GCAGAAGTGG TCATTGACTG CACTTGCGGT GGGTGGTGAG 
CAACTTACCT TTTGGCAACT ATTTAATGAA ACAATGGCAA AATTAAAAAC CAAATTGGAT 
ACATTTGCTT TAACCACATT ATCATTGGCT GAATTCTCAA ATTGGCTGCT ACATGCGCTC 
GTGCGGCTGT TTGGTTATCG CTCTAATGGG CAGTTTGTAG GTCCAAGGCA TGAGCGCGCT 
GTGGCTGAAA CGGCGGAGAA TTTGGACGCG CTGGAGCAAT GTACACAACA GTTCCTCAAC 
GGTAATCCCA GCGAAGAGCA GACACGCGTT TATATTTGCC TAATAATGCC TACGCTGTTG 
CATTGGTGGC GTCCACGCGT CACAATACCA ATTCTTTTGT GGGAGCATTT CCACAAGCGT 
CTAAATACTA CATTCTATGC TGCCGGTTCG GCACCAAGTA ATTTGGCTGT CGCTTGTGCC 
AGCGGCAAGG CATATGTGGC GCGTTATCGC GCGCTATTGC AGCAAGAAGA ATTCACACCT 
GATGCCAATT TGAGTAGTTA CACGCTGTTC ACGTTATTGC TGGGCAAGAA TCTAATGCGC 
TTGCTGGAAA ACGCAGCGAC GCGTCATCAG GCGCAAAAAC TGCTCGGCCG CATTTATACC 
AAATTCAGCG CGCAAAAGTT TCTCGCGCTA AATGAAATGG GCATACACCA TCTGATTGAG 
TTATTCCTGG CGCTAGCGCT TTGTGGTGAT TTCGCAGAGC TTGCGCCGAA GCTGAGCGCG 
AAATTGCTAT CCATCGCCTT GGATAAAATA CCTGCTGGAC GTCAAATTGC AGTAACTAAA 
GGTCATATGG CGCTATTGAT ACTCTATGCG GAGCAGCGCT GTGAAATTGG CGAATATGTG 
ACGAAACTGT TGCAACAGCT CGCTGCTATT AATAACGATT TGGGCGTTTC GAAAGTGCTG 
GCAGATGGTT TGTTGGAGAT TTTCATGCAT GCCGACGATT TTCGACGCGG CGAACACTTG 
CTCATAGAGG CGTGGCTGCC GAATTATCTG CAAACCTGCC CGCCGGTGGA ACAAGAACGC 
AGCTTGGAGG CCTTACATCT GATATTCGGC AAATTGCAGC GCAACGAAGC GCTAATCGAG 
TCGAACGCGG CGCTCTTCAA AGCGCTCAAT GCGCATGTGT TGCCGTTTGT CAAGCAACAA 
TTTGCTTGCG GTTACAGCCA ATGGCTGCCG CAGCTCGCCG CAGACTTTTC CACGCACGCC 
TGCACAGTGC CGGACACGCA GACGTTTCAG AAACTATTCA CCTGCTTCAT CGACGCGCCG 
GTTGCATGCA GACAGGCGCT GCCGCAATTT CTCAATAAAG TGTTGCAAAC TGAGCGCGCC 
GCTTTGATTG AGCCGAATGT GGTAATACAT GTGTGGCTGA AGAGTTTGGT GCAGCTGACC 
GCTGTTAACG AGGATGTGGC GCAATTGACG CGCTATGTGT CGCAGTTGGC GGCATTTCGC 
ATGATGACCG ACAATTTGAA TCCCGAAGAG CTGCTGCAGG CAAAAGAACC GCTATGTGTG 
TTCATAGCCG CTGTTGGCAA ACATTACGAC GCATGTGCGT CGGATCATCA ACGGCGTTTT 
CGCATTGCGG ACAATTTGAA TGCATATTTA CGCAATTTCG ACAAATGGCT ACAGTCGGAC 
CTAAAGCAGG AGAAGACCGA AGTGGCCTTT CGCTTCTACA GTTTCCTGGC CATAGTTATA 
TACAATTGCG CGCACATCGT ATACACCAAA TCGAAGGTGA ACTGTTTCTT TCACGTGGTC 
ATGACGCGTT TCGTGCTGCC CACCAATGTG CAGATGGGCA AAGCGCCCGA TGGCAAATTG 
GCGCAAGTGG TGCATAAAAT TTGGCCTGTG CTCGTGCAGG GTATTGGCCG ACTCAATTTC 
AAAGCCGACC CGTATATTAG CAAAACGCTC ACCGACCTCA TACAAAAGTG GACGCCACAT 
TTCAAAATCT CACCGAACGC CAAAGTGGTC GCACGCCCCT TTGTCAGCTG TCTGCAAAGC 
GACAACGCTG AATTGTCGCT ATTTGTCTTC GAGAAGCTAA CCAGCTTGTT CTTGGCCACA 
CAGCGCCGTC AAGCCGATCC GAATGCCTGC CTAGTAGTGA CTATCTTCCA AGAAGTGATC 
GAGTCCATAG ACGCCACCGC CGCCACTAAC GCCAGTGACG ACGTGTTAAC GCAACGTTTG 
GAGACTTTCA TGAAAGCCAC ATCGCTGCTG ATGTTGGAGC ACATTATGAT GGTGGATGAG 
GTGGTGCCCA GTCGCGCGCT TTTACTGGAT CTGTTTAAAC GCATCGTCAG CAGCGCGACA 
TATAAGCGCA CAGCCGCTTT ACAACAACTA CTGGCCGAGC ACTTGCGTAT ACTTACCAAA 
AAGCATCTCG CCTACTACAC TTTCTTCTTC TTCGAGCTGT TGCAGCGTTT GGCGCAATTC 
ACACCGGACA CGATTTTGCA AATCATGGAT TTTCTGCTCG CCGAGTTGCG TGTTGTGGAG 
CAGAAGCGTG GCTCCGGTGA GGATAATCGC ATGAGAGGAT GCTTGCAAAA GTTACAACAG 
ACTTTGAAAT GTGACAGATA G