LOC105208627 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105208627
Entrez Gene ID	105208627
Full Name	ribosomal RNA small subunit methyltransferase NEP1
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011178575.1	XP_011176877.1	ribosomal RNA small subunit methyltransferase NEP1
XM_011178575.2	XP_011176877.1	ribosomal RNA small subunit methyltransferase NEP1

Pathways from BioSystems
Gene Ontology

The following LOC105208627 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105208627 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa194342	XM_011178575.2 Latest version!	Zeugodacus cucurbitae ribosomal RNA small subunit methyltransferase NEP1 (LOC105208627), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OBa194342	XM_011178575.1	Bactrocera cucurbitae ribosomal RNA small subunit methyltransferase NEP1 (LOC105208627), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105208627 ( XM_011178575.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176877.1 Bactrocera cucurbitae ribosomal RNA small subunit methyltransferase NEP1 (LOC105208627), mRNA.

Price & Availability↑

CloneID	OBa194342
Clone ID Related Accession (Same CDS sequence)	XM_011178575.1 , XM_011178575.2
Accession Version	XM_011178575.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	ribosomal RNA small subunit methyltransferase NEP1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863674.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGCA ATAAGTTTGA TAACCATACT GGTGTTAAAA GGAAACGCAA ATTTCAAGGA 
CCGGCTGGAC AGGATGATCC CGAATTTGAT TTGAAGAAAA AGCATTTGGA GACAACACAC 
ATACGCGCAT TGGAGAAACG TTTAATAATT ATTTTGGAAG GTGCACAATT GGAGACCGTA 
AAAGTCGGTA AAGTGTTCGA GCTACTTAAC TGTGACGATC ATCAGAATAT TATGCGTAAA 
AACAATCGTG ATCCTGGTAA ATGTCGTCCC GATATAACGC ATCAATGCCT GTTAATGCTT 
TTCGATTCGC CTTTAAATCG CGCTGGTTTA TTACAAGTGT ATGTGCGTAC AGAGAAAAAT 
GTGCTAATTG AAATTAATCC GCAAACACGT ATACCACGCA CATTTAAACG TTTTGCGGGC 
TTGATGGTGC AACTGTTGCA TAAATTCTCC GTGCGCGCTA GTGACACCTC GGCCATGTTG 
ATGAAGGTCA TAAAGAATCC GATAACAGAT CATTTGCCTG TTGGTTGCAA GAAGTACGCA 
ATGTCTTTTT CGGGCAAGCT CATGTCCAAT TGTCGCGATT TGGTGCCAAG AGTACAAGTT 
AATGGTGAAA ACACAGAGGA AATTTCCGAA GAACCCATCG CGTTAATTGT GGGTGCTTTC 
GCGCACGGTG TGCTCAATCC AGATTATTCG GAAGGCTTAT TTTCCATTAG CAATTATCCA 
TTATCAGCAG CGATCGCATG TAGCAAGTTG TGCAGTGCCT TCGAAGAAGT GTGGGGTGTT 
GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176877.1
CDS	145..930
Translation	MGGNKFDNHTGVKRKRKFQGPAGQDDPEFDLKKKHLETTHIRALEKRLIIILEGAQLETVKVGKVFELLNCDDHQNIMRKNNRDPGKCRPDITHQCLLMLFDSPLNRAGLLQVYVRTEKNVLIEINPQTRIPRTFKRFAGLMVQLLHKFSVRASDTSAMLMKVIKNPITDHLPVGCKKYAMSFSGKLMSNCRDLVPRVQVNGENTEEISEEPIALIVGAFAHGVLNPDYSEGLFSISNYPLSAAIACSKLCSAFEEVWGVV

Target ORF information:

RefSeq Version	XM_011178575.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae ribosomal RNA small subunit methyltransferase NEP1 (LOC105208627), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178575.1

ORF Insert Sequence:

ATGGGTGGCA ATAAGTTTGA TAACCATACT GGTGTTAAAA GGAAACGCAA ATTTCAAGGA 
CCGGCTGGAC AGGATGATCC CGAATTTGAT TTGAAGAAAA AGCATTTGGA GACAACACAC 
ATACGCGCAT TGGAGAAACG TTTAATAATT ATTTTGGAAG GTGCACAATT GGAGACCGTA 
AAAGTCGGTA AAGTGTTCGA GCTACTTAAC TGTGACGATC ATCAGAATAT TATGCGTAAA 
AACAATCGTG ATCCTGGTAA ATGTCGTCCC GATATAACGC ATCAATGCCT GTTAATGCTT 
TTCGATTCGC CTTTAAATCG CGCTGGTTTA TTACAAGTGT ATGTGCGTAC AGAGAAAAAT 
GTGCTAATTG AAATTAATCC GCAAACACGT ATACCACGCA CATTTAAACG TTTTGCGGGC 
TTGATGGTGC AACTGTTGCA TAAATTCTCC GTGCGCGCTA GTGACACCTC GGCCATGTTG 
ATGAAGGTCA TAAAGAATCC GATAACAGAT CATTTGCCTG TTGGTTGCAA GAAGTACGCA 
ATGTCTTTTT CGGGCAAGCT CATGTCCAAT TGTCGCGATT TGGTGCCAAG AGTACAAGTT 
AATGGTGAAA ACACAGAGGA AATTTCCGAA GAACCCATCG CGTTAATTGT GGGTGCTTTC 
GCGCACGGTG TGCTCAATCC AGATTATTCG GAAGGCTTAT TTTCCATTAG CAATTATCCA 
TTATCAGCAG CGATCGCATG TAGCAAGTTG TGCAGTGCCT TCGAAGAAGT GTGGGGTGTT 
GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105208627 ( XM_011178575.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011176877.1 Zeugodacus cucurbitae ribosomal RNA small subunit methyltransferase NEP1 (LOC105208627), mRNA.

Price & Availability↑

CloneID	OBa194342
Clone ID Related Accession (Same CDS sequence)	XM_011178575.1 , XM_011178575.2
Accession Version	XM_011178575.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	ribosomal RNA small subunit methyltransferase NEP1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863674.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011178575.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGCA ATAAGTTTGA TAACCATACT GGTGTTAAAA GGAAACGCAA ATTTCAAGGA 
CCGGCTGGAC AGGATGATCC CGAATTTGAT TTGAAGAAAA AGCATTTGGA GACAACACAC 
ATACGCGCAT TGGAGAAACG TTTAATAATT ATTTTGGAAG GTGCACAATT GGAGACCGTA 
AAAGTCGGTA AAGTGTTCGA GCTACTTAAC TGTGACGATC ATCAGAATAT TATGCGTAAA 
AACAATCGTG ATCCTGGTAA ATGTCGTCCC GATATAACGC ATCAATGCCT GTTAATGCTT 
TTCGATTCGC CTTTAAATCG CGCTGGTTTA TTACAAGTGT ATGTGCGTAC AGAGAAAAAT 
GTGCTAATTG AAATTAATCC GCAAACACGT ATACCACGCA CATTTAAACG TTTTGCGGGC 
TTGATGGTGC AACTGTTGCA TAAATTCTCC GTGCGCGCTA GTGACACCTC GGCCATGTTG 
ATGAAGGTCA TAAAGAATCC GATAACAGAT CATTTGCCTG TTGGTTGCAA GAAGTACGCA 
ATGTCTTTTT CGGGCAAGCT CATGTCCAAT TGTCGCGATT TGGTGCCAAG AGTACAAGTT 
AATGGTGAAA ACACAGAGGA AATTTCCGAA GAACCCATCG CGTTAATTGT GGGTGCTTTC 
GCGCACGGTG TGCTCAATCC AGATTATTCG GAAGGCTTAT TTTCCATTAG CAATTATCCA 
TTATCAGCAG CGATCGCATG TAGCAAGTTG TGCAGTGCCT TCGAAGAAGT GTGGGGTGTT 
GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011176877.1
CDS	180..965
Translation	MGGNKFDNHTGVKRKRKFQGPAGQDDPEFDLKKKHLETTHIRALEKRLIIILEGAQLETVKVGKVFELLNCDDHQNIMRKNNRDPGKCRPDITHQCLLMLFDSPLNRAGLLQVYVRTEKNVLIEINPQTRIPRTFKRFAGLMVQLLHKFSVRASDTSAMLMKVIKNPITDHLPVGCKKYAMSFSGKLMSNCRDLVPRVQVNGENTEEISEEPIALIVGAFAHGVLNPDYSEGLFSISNYPLSAAIACSKLCSAFEEVWGVV

Target ORF information:

RefSeq Version	XM_011178575.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae ribosomal RNA small subunit methyltransferase NEP1 (LOC105208627), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011178575.2

ORF Insert Sequence:

ATGGGTGGCA ATAAGTTTGA TAACCATACT GGTGTTAAAA GGAAACGCAA ATTTCAAGGA 
CCGGCTGGAC AGGATGATCC CGAATTTGAT TTGAAGAAAA AGCATTTGGA GACAACACAC 
ATACGCGCAT TGGAGAAACG TTTAATAATT ATTTTGGAAG GTGCACAATT GGAGACCGTA 
AAAGTCGGTA AAGTGTTCGA GCTACTTAAC TGTGACGATC ATCAGAATAT TATGCGTAAA 
AACAATCGTG ATCCTGGTAA ATGTCGTCCC GATATAACGC ATCAATGCCT GTTAATGCTT 
TTCGATTCGC CTTTAAATCG CGCTGGTTTA TTACAAGTGT ATGTGCGTAC AGAGAAAAAT 
GTGCTAATTG AAATTAATCC GCAAACACGT ATACCACGCA CATTTAAACG TTTTGCGGGC 
TTGATGGTGC AACTGTTGCA TAAATTCTCC GTGCGCGCTA GTGACACCTC GGCCATGTTG 
ATGAAGGTCA TAAAGAATCC GATAACAGAT CATTTGCCTG TTGGTTGCAA GAAGTACGCA 
ATGTCTTTTT CGGGCAAGCT CATGTCCAAT TGTCGCGATT TGGTGCCAAG AGTACAAGTT 
AATGGTGAAA ACACAGAGGA AATTTCCGAA GAACCCATCG CGTTAATTGT GGGTGCTTTC 
GCGCACGGTG TGCTCAATCC AGATTATTCG GAAGGCTTAT TTTCCATTAG CAATTATCCA 
TTATCAGCAG CGATCGCATG TAGCAAGTTG TGCAGTGCCT TCGAAGAAGT GTGGGGTGTT 
GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.