thra cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	thra
Entrez Gene ID	734136
Full Name	thyroid hormone receptor, alpha
Synonyms	TRalpha,c-erba,erba
General protein information	Preferred Names thyroid hormone receptor, alpha Names thyroid hormone receptor alpha thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012952539.1	XP_012807993.1	thyroid hormone receptor alpha isoform X1
NM_001045796.1	NP_001039261.1	thyroid hormone receptor alpha
XM_012952539.2	XP_012807993.1	thyroid hormone receptor alpha isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	THRA	XP_548137.2
Bos taurus (cattle)	THRA	NP_001039794.1
Xenopus tropicalis (tropical clawed frog)	thra	NP_001039261.1
Mus musculus (house mouse)	Thra	NP_835161.1
Homo sapiens (human)	THRA	NP_955366.1
Danio rerio (zebrafish)	thrab	XP_001921013.4
Rattus norvegicus (Norway rat)	Thra	NP_001017960.1
Gallus gallus (chicken)	THRA	NP_990644.1
Danio rerio (zebrafish)	thraa	NP_571471.1

Gene Ontology
Bibliography

Related articles in PubMed

Thyroid Hormone Receptor ?- and ?-Knockout Xenopus tropicalis Tadpoles Reveal Subtype-Specific Roles During Development.
Nakajima K, Tazawa I, Yaoita Y
Endocrinology159(2)733-743(2018 Feb)

Thyroid Hormone Receptor ? Controls Developmental Timing and Regulates the Rate and Coordination of Tissue-Specific Metamorphosis in Xenopus tropicalis.
Wen L, Shibata Y, Su D, Fu L, Luu N, Shi YB
Endocrinology158(6)1985-1998(2017 Jun)

Growth, Development, and Intestinal Remodeling Occurs in the Absence of Thyroid Hormone Receptor ? in Tadpoles of Xenopus tropicalis.
Choi J, Ishizuya-Oka A, Buchholz DR
Endocrinology158(6)1623-1633(2017 Jun)

Unliganded thyroid hormone receptor ? regulates developmental timing via gene repression in Xenopus tropicalis.
Choi J, Suzuki KT, Sakuma T, Shewade L, Yamamoto T, Buchholz DR
Endocrinology156(2)735-44(2015 Feb)

Unliganded thyroid hormone receptor ? controls developmental timing in Xenopus tropicalis.
Wen L, Shi YB
Endocrinology156(2)721-34(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A critical role of endogenous TRalpha, not only in regulating both the timing and rate of metamorphosis, but also in coordinating temporal metamorphosis of different organs.
Title: Thyroid Hormone Receptor ? Controls Developmental Timing and Regulates the Rate and Coordination of Tissue-Specific Metamorphosis in Xenopus tropicalis.

As in the TRalpha null mouse model, TRalphaKO frogs had statistically significant but surprisingly mild growth and development phenotypes with normal survival and fertility.
Title: Growth, Development, and Intestinal Remodeling Occurs in the Absence of Thyroid Hormone Receptor ? in Tadpoles of Xenopus tropicalis.

unliganded TRalpha delays developmental progression by repressing TH-response genes
Title: Unliganded thyroid hormone receptor ? regulates developmental timing via gene repression in Xenopus tropicalis.

roles in tadpole growth rate and the timing of metamorphosis
Title: Unliganded thyroid hormone receptor ? controls developmental timing in Xenopus tropicalis.

The following thra gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the thra cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa25333	NM_001045796.1 Latest version!	Xenopus tropicalis thyroid hormone receptor alpha (thra), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OXa28865	XM_012952539.2 Latest version!	Xenopus tropicalis thyroid hormone receptor alpha (thra), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OXa28865	XM_012952539.1	Xenopus tropicalis thyroid hormone receptor, alpha (thra), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

thra ( NM_001045796.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001039261.1 Xenopus tropicalis thyroid hormone receptor alpha (thra), mRNA.

Price & Availability↑

CloneID	OXa25333
Clone ID Related Accession (Same CDS sequence)	NM_001045796.1
Accession Version	NM_001045796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	thyroid hormone receptor alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB244213.1. ##Evidence-Data-START## Transcript exon combination :: AB244213.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028294, SAMD00028295 [ECO:0000350] ##Evidence-Data-END##

ATGGACCAGA ATCTCAGCGG GCTGGACTGC TTGTCAGAGC CAGATGAAAA AAGGTGGCCG 
GATGGGAAGC GGAAAAGAAA GAACAGCCAA TGTATGGGAA AAAGCGGCAT GTCCGGTGAC 
AGCTTGGTGT CTCTGCCCTC TGCAGGGTAC ATCCCCAGCT ATCTGGACAA AGACGAGCCG 
TGTGTGGTGT GCAGTGATAA GGCCACGGGG TACCACTACC GCTGTATCAC TTGCGAGGGG 
TGTAAGGGTT TCTTCCGTCG CACCATCCAG AAGAACCTGC ACCCCTCCTA TTCATGCAAG 
TACGATGGCT GCTGCATTAT CGACAAGATC ACCCGAAACC AGTGCCAGCT CCGCCGCTTC 
AAGAAATGCA TTGCCGTTGG CATGGCAATG GATCTTGTCC TGGATGATAC CAAGCGGGTA 
GCCAAGCGAA AACTGATTGA AGAGAATCGA GAGCGGCGGC GGAAGGAGGA GATGATCAAG 
ACTCTGCAAC AGCGTCCAGA GCCCAGCAAA GAGGAGTGGG AGTTGATTCG CATCGTAACA 
GAAGCTCACA GAAGCACCAA CGCTCAGGGC AGCCACTGGA AACAGCGTAG GAAGTTTCTG 
CCGGAAGATA TCGGGCAGTC TCCCATGGCT TCCATGCCAG ATGGGGATAA AGTTGACTTG 
GAGGCTTTCA GTGAGTTCAC CAAAATAATA ACCCCAGCAA TTACGAGAGT GGTGGACTTT 
GCTAAGAAGC TGCCCATGTT CTCTGAGCTG CCTTGTGAAG ATCAGATCAT CCTGTTGAAA 
GGATGCTGTA TGGAGATCAT GTCTCTCCGT GCTGCTGTGC GCTACGATCC GGACAGCGAG 
ACCCTAACGC TAAGCGGTGA GATGGCGGTA AAAAGGGAGC AGCTTAAGAA CGGAGGTCTG 
GGTGCTGTCT CGGATGCCAT CTTTGACCTT GGAAGATCGC TTGCTGCATT CAACCTTGAC 
GACACGGAAG TGGCGCTGCT GCAGGCTGTT TTGCTCATGT CATCAGACCG TACTGGTTTA 
ATGTGCACGG ACAAGATAGA GAAATGTCAA GAGACCTACC TTCTCGCCTT TGAACACTAC 
ATCAACCATC GCAAACACAA CATTCCCCAC TTCTGGCCCA AGCTCCTAAT GAAGGTGACG 
GACCTGCGCA TGATAGGGGC ATGCCATGCC AGCCGCTTTC TGCACATGAA GGTTGAGTGC 
CCCACTGAGC TCTTTCCACC GCTCTTCCTT GAGGTCTTTG AGGACCAGGA AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001039261.1
CDS	304..1560
Translation	MDQNLSGLDCLSEPDEKRWPDGKRKRKNSQCMGKSGMSGDSLVSLPSAGYIPSYLDKDEPCVVCSDKATGYHYRCITCEGCKGFFRRTIQKNLHPSYSCKYDGCCIIDKITRNQCQLRRFKKCIAVGMAMDLVLDDTKRVAKRKLIEENRERRRKEEMIKTLQQRPEPSKEEWELIRIVTEAHRSTNAQGSHWKQRRKFLPEDIGQSPMASMPDGDKVDLEAFSEFTKIITPAITRVVDFAKKLPMFSELPCEDQIILLKGCCMEIMSLRAAVRYDPDSETLTLSGEMAVKREQLKNGGLGAVSDAIFDLGRSLAAFNLDDTEVALLQAVLLMSSDRTGLMCTDKIEKCQETYLLAFEHYINHRKHNIPHFWPKLLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFEDQEV

Target ORF information:

RefSeq Version	NM_001045796.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis thyroid hormone receptor alpha (thra), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045796.1

ORF Insert Sequence:

ATGGACCAGA ATCTCAGCGG GCTGGACTGC TTGTCAGAGC CAGATGAAAA AAGGTGGCCG 
GATGGGAAGC GGAAAAGAAA GAACAGCCAA TGTATGGGAA AAAGCGGCAT GTCCGGTGAC 
AGCTTGGTGT CTCTGCCCTC TGCAGGGTAC ATCCCCAGCT ATCTGGACAA AGACGAGCCG 
TGTGTGGTGT GCAGTGATAA GGCCACGGGG TACCACTACC GCTGTATCAC TTGCGAGGGG 
TGTAAGGGTT TCTTCCGTCG CACCATCCAG AAGAACCTGC ACCCCTCCTA TTCATGCAAG 
TACGATGGCT GCTGCATTAT CGACAAGATC ACCCGAAACC AGTGCCAGCT CCGCCGCTTC 
AAGAAATGCA TTGCCGTTGG CATGGCAATG GATCTTGTCC TGGATGATAC CAAGCGGGTA 
GCCAAGCGAA AACTGATTGA AGAGAATCGA GAGCGGCGGC GGAAGGAGGA GATGATCAAG 
ACTCTGCAAC AGCGTCCAGA GCCCAGCAAA GAGGAGTGGG AGTTGATTCG CATCGTAACA 
GAAGCTCACA GAAGCACCAA CGCTCAGGGC AGCCACTGGA AACAGCGTAG GAAGTTTCTG 
CCGGAAGATA TCGGGCAGTC TCCCATGGCT TCCATGCCAG ATGGGGATAA AGTTGACTTG 
GAGGCTTTCA GTGAGTTCAC CAAAATAATA ACCCCAGCAA TTACGAGAGT GGTGGACTTT 
GCTAAGAAGC TGCCCATGTT CTCTGAGCTG CCTTGTGAAG ATCAGATCAT CCTGTTGAAA 
GGATGCTGTA TGGAGATCAT GTCTCTCCGT GCTGCTGTGC GCTACGATCC GGACAGCGAG 
ACCCTAACGC TAAGCGGTGA GATGGCGGTA AAAAGGGAGC AGCTTAAGAA CGGAGGTCTG 
GGTGCTGTCT CGGATGCCAT CTTTGACCTT GGAAGATCGC TTGCTGCATT CAACCTTGAC 
GACACGGAAG TGGCGCTGCT GCAGGCTGTT TTGCTCATGT CATCAGACCG TACTGGTTTA 
ATGTGCACGG ACAAGATAGA GAAATGTCAA GAGACCTACC TTCTCGCCTT TGAACACTAC 
ATCAACCATC GCAAACACAA CATTCCCCAC TTCTGGCCCA AGCTCCTAAT GAAGGTGACG 
GACCTGCGCA TGATAGGGGC ATGCCATGCC AGCCGCTTTC TGCACATGAA GGTTGAGTGC 
CCCACTGAGC TCTTTCCACC GCTCTTCCTT GAGGTCTTTG AGGACCAGGA AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

thra ( XM_012952539.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012807993.1 Xenopus tropicalis thyroid hormone receptor, alpha (thra), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa28865
Clone ID Related Accession (Same CDS sequence)	XM_012952539.1 , XM_012952539.2
Accession Version	XM_012952539.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	thyroid hormone receptor alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683386.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGA ATCTCAGCGG GCTGGACTGC TTGTCAGAGC CAGATGAAAA AAGGTGGCCG 
GATGGGAAGC GAAAAAGAAA GAACAGCCAA TGTATGGGAA AAAGCGGCAT GTCCGGTGAC 
AGCTTGGTGT CTCTGCCCTC TGCAGGGTAC ATCCCCAGCT ATCTGGACAA AGACGAGCCG 
TGTGTGGTGT GCAGTGATAA GGCCACGGGG TACCACTACC GCTGTATCAC TTGCGAGGGG 
TGTAAGGGTT TCTTCCGTCG CACCATCCAG AAGAACCTGC ACCCCTCCTA TTCATGCAAG 
TACGATGGCT GCTGCATTAT CGACAAGATC ACCCGAAACC AGTGCCAGCT CTGCCGCTTC 
AAGAAATGCA TTGCCGTTGG CATGGCAATG GATCTTGTCC TGGATGATAC CAAGCGGGTA 
GCCAAGCGAA AACTGATTGA AGAGAATCGA GAGCGGCGGC GGAAGGAGGA GATGATCAAG 
ACTCTGCAAC AGCGTCCAGA GCCCAGCAAA GAGGAGTGGG AGTTGATTCG CATCGTAACA 
GAAGCTCACA GGAGCACCAA CGCTCAGGGC AGCCACTGGA AACAGCGTAG GAAGTTTCTG 
CCGGAAGATA TCGGGCAGTC TCCCATGGCT TCCATGCCAG ATGGGGATAA AGTTGACTTG 
GAGGCTTTCA GTGAGTTCAC CAAAATAATA ACCCCAGCAA TTACGAGAGT GGTGGACTTT 
GCTAAGAAGC TGCCCATGTT CTCTGAGCTG CCTTGTGAAG ATCAGATCAT CCTGTTGAAA 
GGATGCTGTA TGGAGATCAT GTCTCTCCGT GCTGCTGTGC GCTACGATCC GGACAGCGAG 
ACCCTAACGC TAAGCGGTGA GATGGCGGTA AAAAGGGAGC AGCTTAAGAA CGGAGGTCTG 
GGTGTTGTCT CGGATGCCAT CTTTGACCTT GGAAGATCGC TTGCTGCATT CAACCTTGAC 
GACACGGAAG TGGCGCTGCT GCAGGCTGTT TTGCTCATGT CATCAGACCG TACTGGTTTA 
ATGTGCACGG ACAAGATAGA GAAATGTCAA GAGACCTACC TTCTCGCCTT TGAACACTAC 
ATCAACCATC GCAAACACAA CATTCCCCAC TTCTGGCCCA AGCTCCTAAT GAAGGTGACG 
GACCTGCGCA TGATAGGGGC ATGCCATGCC AGCCGCTTTC TGCACATGAA GGTTGAGTGC 
CCCACTGAGC TCTTTCCACC GCTCTTCCTT GAGGTCTTTG AGGACCAGGA AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012807993.1
CDS	637..1893
Translation	MDQNLSGLDCLSEPDEKRWPDGKRKRKNSQCMGKSGMSGDSLVSLPSAGYIPSYLDKDEPCVVCSDKATGYHYRCITCEGCKGFFRRTIQKNLHPSYSCKYDGCCIIDKITRNQCQLCRFKKCIAVGMAMDLVLDDTKRVAKRKLIEENRERRRKEEMIKTLQQRPEPSKEEWELIRIVTEAHRSTNAQGSHWKQRRKFLPEDIGQSPMASMPDGDKVDLEAFSEFTKIITPAITRVVDFAKKLPMFSELPCEDQIILLKGCCMEIMSLRAAVRYDPDSETLTLSGEMAVKREQLKNGGLGVVSDAIFDLGRSLAAFNLDDTEVALLQAVLLMSSDRTGLMCTDKIEKCQETYLLAFEHYINHRKHNIPHFWPKLLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFEDQEV

Target ORF information:

RefSeq Version	XM_012952539.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis thyroid hormone receptor, alpha (thra), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952539.1

ORF Insert Sequence:

ATGGACCAGA ATCTCAGCGG GCTGGACTGC TTGTCAGAGC CAGATGAAAA AAGGTGGCCG 
GATGGGAAGC GAAAAAGAAA GAACAGCCAA TGTATGGGAA AAAGCGGCAT GTCCGGTGAC 
AGCTTGGTGT CTCTGCCCTC TGCAGGGTAC ATCCCCAGCT ATCTGGACAA AGACGAGCCG 
TGTGTGGTGT GCAGTGATAA GGCCACGGGG TACCACTACC GCTGTATCAC TTGCGAGGGG 
TGTAAGGGTT TCTTCCGTCG CACCATCCAG AAGAACCTGC ACCCCTCCTA TTCATGCAAG 
TACGATGGCT GCTGCATTAT CGACAAGATC ACCCGAAACC AGTGCCAGCT CTGCCGCTTC 
AAGAAATGCA TTGCCGTTGG CATGGCAATG GATCTTGTCC TGGATGATAC CAAGCGGGTA 
GCCAAGCGAA AACTGATTGA AGAGAATCGA GAGCGGCGGC GGAAGGAGGA GATGATCAAG 
ACTCTGCAAC AGCGTCCAGA GCCCAGCAAA GAGGAGTGGG AGTTGATTCG CATCGTAACA 
GAAGCTCACA GGAGCACCAA CGCTCAGGGC AGCCACTGGA AACAGCGTAG GAAGTTTCTG 
CCGGAAGATA TCGGGCAGTC TCCCATGGCT TCCATGCCAG ATGGGGATAA AGTTGACTTG 
GAGGCTTTCA GTGAGTTCAC CAAAATAATA ACCCCAGCAA TTACGAGAGT GGTGGACTTT 
GCTAAGAAGC TGCCCATGTT CTCTGAGCTG CCTTGTGAAG ATCAGATCAT CCTGTTGAAA 
GGATGCTGTA TGGAGATCAT GTCTCTCCGT GCTGCTGTGC GCTACGATCC GGACAGCGAG 
ACCCTAACGC TAAGCGGTGA GATGGCGGTA AAAAGGGAGC AGCTTAAGAA CGGAGGTCTG 
GGTGTTGTCT CGGATGCCAT CTTTGACCTT GGAAGATCGC TTGCTGCATT CAACCTTGAC 
GACACGGAAG TGGCGCTGCT GCAGGCTGTT TTGCTCATGT CATCAGACCG TACTGGTTTA 
ATGTGCACGG ACAAGATAGA GAAATGTCAA GAGACCTACC TTCTCGCCTT TGAACACTAC 
ATCAACCATC GCAAACACAA CATTCCCCAC TTCTGGCCCA AGCTCCTAAT GAAGGTGACG 
GACCTGCGCA TGATAGGGGC ATGCCATGCC AGCCGCTTTC TGCACATGAA GGTTGAGTGC 
CCCACTGAGC TCTTTCCACC GCTCTTCCTT GAGGTCTTTG AGGACCAGGA AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

thra ( XM_012952539.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012807993.1 Xenopus tropicalis thyroid hormone receptor alpha (thra), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa28865
Clone ID Related Accession (Same CDS sequence)	XM_012952539.1 , XM_012952539.2
Accession Version	XM_012952539.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	thyroid hormone receptor alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952539.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGA ATCTCAGCGG GCTGGACTGC TTGTCAGAGC CAGATGAAAA AAGGTGGCCG 
GATGGGAAGC GAAAAAGAAA GAACAGCCAA TGTATGGGAA AAAGCGGCAT GTCCGGTGAC 
AGCTTGGTGT CTCTGCCCTC TGCAGGGTAC ATCCCCAGCT ATCTGGACAA AGACGAGCCG 
TGTGTGGTGT GCAGTGATAA GGCCACGGGG TACCACTACC GCTGTATCAC TTGCGAGGGG 
TGTAAGGGTT TCTTCCGTCG CACCATCCAG AAGAACCTGC ACCCCTCCTA TTCATGCAAG 
TACGATGGCT GCTGCATTAT CGACAAGATC ACCCGAAACC AGTGCCAGCT CTGCCGCTTC 
AAGAAATGCA TTGCCGTTGG CATGGCAATG GATCTTGTCC TGGATGATAC CAAGCGGGTA 
GCCAAGCGAA AACTGATTGA AGAGAATCGA GAGCGGCGGC GGAAGGAGGA GATGATCAAG 
ACTCTGCAAC AGCGTCCAGA GCCCAGCAAA GAGGAGTGGG AGTTGATTCG CATCGTAACA 
GAAGCTCACA GGAGCACCAA CGCTCAGGGC AGCCACTGGA AACAGCGTAG GAAGTTTCTG 
CCGGAAGATA TCGGGCAGTC TCCCATGGCT TCCATGCCAG ATGGGGATAA AGTTGACTTG 
GAGGCTTTCA GTGAGTTCAC CAAAATAATA ACCCCAGCAA TTACGAGAGT GGTGGACTTT 
GCTAAGAAGC TGCCCATGTT CTCTGAGCTG CCTTGTGAAG ATCAGATCAT CCTGTTGAAA 
GGATGCTGTA TGGAGATCAT GTCTCTCCGT GCTGCTGTGC GCTACGATCC GGACAGCGAG 
ACCCTAACGC TAAGCGGTGA GATGGCGGTA AAAAGGGAGC AGCTTAAGAA CGGAGGTCTG 
GGTGTTGTCT CGGATGCCAT CTTTGACCTT GGAAGATCGC TTGCTGCATT CAACCTTGAC 
GACACGGAAG TGGCGCTGCT GCAGGCTGTT TTGCTCATGT CATCAGACCG TACTGGTTTA 
ATGTGCACGG ACAAGATAGA GAAATGTCAA GAGACCTACC TTCTCGCCTT TGAACACTAC 
ATCAACCATC GCAAACACAA CATTCCCCAC TTCTGGCCCA AGCTCCTAAT GAAGGTGACG 
GACCTGCGCA TGATAGGGGC ATGCCATGCC AGCCGCTTTC TGCACATGAA GGTTGAGTGC 
CCCACTGAGC TCTTTCCACC GCTCTTCCTT GAGGTCTTTG AGGACCAGGA AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012807993.1
CDS	640..1896
Translation	MDQNLSGLDCLSEPDEKRWPDGKRKRKNSQCMGKSGMSGDSLVSLPSAGYIPSYLDKDEPCVVCSDKATGYHYRCITCEGCKGFFRRTIQKNLHPSYSCKYDGCCIIDKITRNQCQLCRFKKCIAVGMAMDLVLDDTKRVAKRKLIEENRERRRKEEMIKTLQQRPEPSKEEWELIRIVTEAHRSTNAQGSHWKQRRKFLPEDIGQSPMASMPDGDKVDLEAFSEFTKIITPAITRVVDFAKKLPMFSELPCEDQIILLKGCCMEIMSLRAAVRYDPDSETLTLSGEMAVKREQLKNGGLGVVSDAIFDLGRSLAAFNLDDTEVALLQAVLLMSSDRTGLMCTDKIEKCQETYLLAFEHYINHRKHNIPHFWPKLLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFEDQEV

Target ORF information:

RefSeq Version	XM_012952539.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis thyroid hormone receptor alpha (thra), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952539.2

ORF Insert Sequence:

ATGGACCAGA ATCTCAGCGG GCTGGACTGC TTGTCAGAGC CAGATGAAAA AAGGTGGCCG 
GATGGGAAGC GAAAAAGAAA GAACAGCCAA TGTATGGGAA AAAGCGGCAT GTCCGGTGAC 
AGCTTGGTGT CTCTGCCCTC TGCAGGGTAC ATCCCCAGCT ATCTGGACAA AGACGAGCCG 
TGTGTGGTGT GCAGTGATAA GGCCACGGGG TACCACTACC GCTGTATCAC TTGCGAGGGG 
TGTAAGGGTT TCTTCCGTCG CACCATCCAG AAGAACCTGC ACCCCTCCTA TTCATGCAAG 
TACGATGGCT GCTGCATTAT CGACAAGATC ACCCGAAACC AGTGCCAGCT CTGCCGCTTC 
AAGAAATGCA TTGCCGTTGG CATGGCAATG GATCTTGTCC TGGATGATAC CAAGCGGGTA 
GCCAAGCGAA AACTGATTGA AGAGAATCGA GAGCGGCGGC GGAAGGAGGA GATGATCAAG 
ACTCTGCAAC AGCGTCCAGA GCCCAGCAAA GAGGAGTGGG AGTTGATTCG CATCGTAACA 
GAAGCTCACA GGAGCACCAA CGCTCAGGGC AGCCACTGGA AACAGCGTAG GAAGTTTCTG 
CCGGAAGATA TCGGGCAGTC TCCCATGGCT TCCATGCCAG ATGGGGATAA AGTTGACTTG 
GAGGCTTTCA GTGAGTTCAC CAAAATAATA ACCCCAGCAA TTACGAGAGT GGTGGACTTT 
GCTAAGAAGC TGCCCATGTT CTCTGAGCTG CCTTGTGAAG ATCAGATCAT CCTGTTGAAA 
GGATGCTGTA TGGAGATCAT GTCTCTCCGT GCTGCTGTGC GCTACGATCC GGACAGCGAG 
ACCCTAACGC TAAGCGGTGA GATGGCGGTA AAAAGGGAGC AGCTTAAGAA CGGAGGTCTG 
GGTGTTGTCT CGGATGCCAT CTTTGACCTT GGAAGATCGC TTGCTGCATT CAACCTTGAC 
GACACGGAAG TGGCGCTGCT GCAGGCTGTT TTGCTCATGT CATCAGACCG TACTGGTTTA 
ATGTGCACGG ACAAGATAGA GAAATGTCAA GAGACCTACC TTCTCGCCTT TGAACACTAC 
ATCAACCATC GCAAACACAA CATTCCCCAC TTCTGGCCCA AGCTCCTAAT GAAGGTGACG 
GACCTGCGCA TGATAGGGGC ATGCCATGCC AGCCGCTTTC TGCACATGAA GGTTGAGTGC 
CCCACTGAGC TCTTTCCACC GCTCTTCCTT GAGGTCTTTG AGGACCAGGA AGTCTGA