adam33 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	adam33
Entrez Gene ID	677728
Full Name	ADAM metallopeptidase domain 33
Synonyms	adam13
General protein information	Preferred Names ADAM metallopeptidase domain 33 Names ADAM metallopeptidase domain 33 ADAM metallopeptidase domain 13 x-adam 13
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012963957.2	XP_012819411.1	ADAM metallopeptidase domain 33 isoform X1
XM_012963962.2	XP_012819416.1	ADAM metallopeptidase domain 33 isoform X2
XM_012963957.3	XP_012819411.2	ADAM metallopeptidase domain 33 isoform X1
NM_001040013.2	NP_001035102.1	ADAM metallopeptidase domain 33 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	ADAM33	NP_001269376.1
Bos taurus (cattle)	ADAM33	XP_005214954.1
Xenopus tropicalis (tropical clawed frog)	adam33	NP_001035102.1
Pan troglodytes (chimpanzee)	ADAM33	XP_514485.3
Rattus norvegicus (Norway rat)	Adam33	XP_003749622.1
Danio rerio (zebrafish)	adam19a	XP_005172418.1
Canis lupus familiaris (dog)	ADAM33	XP_005634894.1
Mus musculus (house mouse)	Adam33	NP_291093.2
Gallus gallus (chicken)	ADAM33	NP_001075887.1

Gene Ontology
Bibliography

Related articles in PubMed

Roles of ADAM13-regulated Wnt activity in early Xenopus eye development.
Wei S, Xu G, Bridges LC, Williams P, Nakayama T, Shah A, Grainger RM, White JM, DeSimone DW
Developmental biology363(1)147-54(2012 Mar)

ADAM13 induces cranial neural crest by cleaving class B Ephrins and regulating Wnt signaling.
Wei S, Xu G, Bridges LC, Williams P, White JM, DeSimone DW
Developmental cell19(2)345-52(2010 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest an important role of ADAM13-regulated Wnt activity in eye development.
Title: Roles of ADAM13-regulated Wnt activity in early Xenopus eye development.

Data suggest that by cleaving class B ephrins, ADAM13 promotes canonical Wnt signaling and early cranial neural crest induction.
Title: ADAM13 induces cranial neural crest by cleaving class B Ephrins and regulating Wnt signaling.

The following adam33 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the adam33 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa20920	NM_001040013.2 Latest version!	Xenopus tropicalis ADAM metallopeptidase domain 33 (adam33), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa28447	XM_012963957.2 Latest version!	Xenopus tropicalis ADAM metallopeptidase domain 33 (adam33), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa28448	XM_012963962.2 Latest version!	Xenopus tropicalis ADAM metallopeptidase domain 33 (adam33), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa47959	XM_012963957.3 Latest version!	Xenopus tropicalis ADAM metallopeptidase domain 33 (adam33), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

adam33 ( NM_001040013.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001035102.1 Xenopus tropicalis ADAM metallopeptidase domain 33 (adam33), mRNA.

Price & Availability↑

CloneID	OXa20920
Clone ID Related Accession (Same CDS sequence)	NM_001040013.2
Accession Version	NM_001040013.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ADAM metallopeptidase domain 33 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ393788.1. On Apr 13, 2016 this sequence version replaced NM_001040013.1. ##Evidence-Data-START## Transcript exon combination :: DQ393788.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGGGACGG AGGGTTGGCT GCACACATGG CTGGGACTGG GGGCTGTGTT TGTGGGGCTG 
CTCCTGGCTC CGGTGGCGGC ACTGGGGGCC CACCAAGGAG AGCGGGTGAC TGCCCTCTGG 
CTACAGAATG AAAGAGCTAA GAGATCAGTA GAGCTGCTGG ATGAGGGGAC TCCAGACCGG 
GGAGAAATAC TGGTGACATC AGAAGGAAGG CGGTTGATCC TGAAAGTGGA GAGAAATCAC 
CGCCTGTTTG CGCCCGGCTA CACTGAGACA CATTACACTG ATGGGCAGAT GGTCACATTG 
TCTCCCAATC ACACGGAGCA TTGCTATTAC CATGGACAAG TGCAAGGCTA TGATGACTCC 
TCTGTCGCTC TCACCACCTG CTCAGGAATC AGTGGTTTGA TTGTGCTGAG AACTAATGAC 
AGCTACTACC TGAGTCCCCT GGAGGTATCA GGTAAAGAAA CTCATTCACT GGTCCGCACT 
GAACACCTGC CGATCAAAGG TGGGAGCTGT GGGCATGAAG GACATTCAGG AAGCACCACC 
TCTTACTTCA AGGAGTTTAC AGCACCTCCT GGCCATCACA GGGTAAGGAG GAACGTCTGG 
AGGTCCCAGA AATACATGGA GCTATTTATT GTTGCAGATT ACAGCATGTT TATGAAACAG 
AATCGGAATC TAGGCAGCAC GAAGCAACGT GTCCTTGAAA TTGCCAATTA TGTAGACAAG 
TTCTACATGT CCATGAACAT CAAGGTGGCT CTGATAGGGC TGGAAGTCTG GACAGAGAGG 
GATCAATGTG ATGTGAATGA TGATGCCAAC GACAGCCTGA GATCGTTCCT ACAGTGGAAG 
CAAAAGTTGA GGTCCCGGAA GAAGCACGAT AACGCGCAGC TAATCACAGG GGTGACGTTT 
AAAGGAACAA CCATTGGGAT GGCACCACTG GAAGGCATGT GCACTGCTGA GAACTCAGGG 
GGAGTCAGTA TGGATCACTC AGAAAATGCA ATTGGAGCTG CTGCAACAAT GGCCCATGAA 
ATTGGACACA ATTTTGGAAT GAGCCACGAC AATGACGGCT GCTGTGTGGA GGCCACTCCG 
GAACAAGGAG GCTGTATCAT GGCGGCTTCC ACTGGGCATC CCTTCCCTCG CAAGTTCAGC 
CGGTGCAGTC AGCAGCAGCT TATGAGTTAT TTCAGGAAGG GGGGTGGAAT GTGCCTGTTC 
AACATGCCAA ACACCAATGA CCTCGTGATG GGGAAAAAGT GTGGAAATGG GTTCCTGGAG 
GAAGGAGAGC AGTGTGACTG TGGGGAGCCA GAGGAATGCA CTAATCCCTG CTGCAATGCA 
AATAACTGCA CTTTAAAGGC CGGCGCCAAG TGCGCTCATG GCGAATGTTG CCAGGATTGT 
AGGCTGAAAT CTGCAGGGAC TCAGTGCCGG GAAATGGCTG GATCCTGTGA CCTTCCAGAA 
TTCTGCACGG GGGATGCCCC TTTCTGTCCC TCCAATGTGT ATATGCTGGA TGGGTCCTCA 
TGTGCTTTTG GGGAGGCATA CTGTTATAAT GGCATGTGTC TCACTCACCA GCAGCAGTGT 
ATCCATCTTT GGGGTTCAGG AGCTGTGGTT GCCCCTGACT TCTGCTTCCA AGATGTGAAC 
AAAGCAGGAG ACCAGTATGG AAACTGCGGC AAGAATGGAA GAGGACAATT TGTGAAGTGT 
ACATCCCGAG ATGCTAAGTG TGGAAAAATC CAATGCCAGA CGAGCTCAGA AAAGCCCAGG 
GACCCCAGCA TGGTGAAGGT GGACAACACC ATTATCATTA ATGGCTACAA GATGAAGTGT 
CAGGGGGTAC ATGCCTACTC CATGCAAGAA GAGGAAGGTG ACCCGGGGCT CGTAATGACT 
GGGACCAAGT GTGGAGACGG CATGGTATGC AAGGATCGTC GCTGTCAGAA TGCCTCCTTC 
TTTGAGCTGG ACCAGTGCGT GTCTAAGTGT AATGGACATG GGGTCTGTAA CAGCAACAGA 
AACTGCCATT GTCATTCAGG CTGGGCCCCT CCTTACTGTG ACAAACCTGG GCCAGGTGGC 
AGTCAAGACA GTGGCCCTGC ACCTAATGAC ATTCCTGTGG GTGTCACTAT TTTCTTGGTT 
ATCCTGTTCC TGGTTCTGCT GCTGGGTTTG GCTATTGCGA TGGTCTACTG GTATCGAAAA 
CCAGGTTCTC TCCTGAACAA GTGGCTGAAG AAGAGCAAAG CAAAATGCAG CTTATGCAAA 
GCCACCCAAC CAAAGGCAAA CAGGGCGTAT TCAAGCAAGA TCTTTACTAT GCGCGATATA 
TCCTATCCAG TTAAATCCGA CAGCAAACAA ACTCACTCAC GGGACATATT CCAAGGAAAA 
ACAACTGCTG CTCAGAACAG TTCTCATCCT GTTAACGTTG TGCGTCCTCT TCGTCCGGCT 
CCCAGCCCAG TGATCCAACA TGGGAAGCCT CTTAGGCCTC CTCCTCCTCC AATGAAACCG 
TCGCCCATTC TGCCTGCAAA AGAAGAAACC GTTCATGTAA AACTGCTACC CCCCAAGAAA 
CCTCTACCCT CTTGCCCTAG GCCGCAGCAG CTGAACCCAC CTTCCAAGCC ACTGCCGGTG 
ACTCCTGCTC ACAAAGAGCC ACTGTTGGTT ATGACACCGG CCACACATAA CAAGGCTCCC 
ATCACCAACT CTGCTACCCA GCTTAAAGGG CCACGCAGGC CAATACAAGG GGGCAAGGTC 
CAAGCTGCTG CTGCTGCCTT TCTTCAGAGA AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035102.1
CDS	5..2740
Translation	MGTEGWLHTWLGLGAVFVGLLLAPVAALGAHQGERVTALWLQNERAKRSVELLDEGTPDRGEILVTSEGRRLILKVERNHRLFAPGYTETHYTDGQMVTLSPNHTEHCYYHGQVQGYDDSSVALTTCSGISGLIVLRTNDSYYLSPLEVSGKETHSLVRTEHLPIKGGSCGHEGHSGSTTSYFKEFTAPPGHHRVRRNVWRSQKYMELFIVADYSMFMKQNRNLGSTKQRVLEIANYVDKFYMSMNIKVALIGLEVWTERDQCDVNDDANDSLRSFLQWKQKLRSRKKHDNAQLITGVTFKGTTIGMAPLEGMCTAENSGGVSMDHSENAIGAAATMAHEIGHNFGMSHDNDGCCVEATPEQGGCIMAASTGHPFPRKFSRCSQQQLMSYFRKGGGMCLFNMPNTNDLVMGKKCGNGFLEEGEQCDCGEPEECTNPCCNANNCTLKAGAKCAHGECCQDCRLKSAGTQCREMAGSCDLPEFCTGDAPFCPSNVYMLDGSSCAFGEAYCYNGMCLTHQQQCIHLWGSGAVVAPDFCFQDVNKAGDQYGNCGKNGRGQFVKCTSRDAKCGKIQCQTSSEKPRDPSMVKVDNTIIINGYKMKCQGVHAYSMQEEEGDPGLVMTGTKCGDGMVCKDRRCQNASFFELDQCVSKCNGHGVCNSNRNCHCHSGWAPPYCDKPGPGGSQDSGPAPNDIPVGVTIFLVILFLVLLLGLAIAMVYWYRKPGSLLNKWLKKSKAKCSLCKATQPKANRAYSSKIFTMRDISYPVKSDSKQTHSRDIFQGKTTAAQNSSHPVNVVRPLRPAPSPVIQHGKPLRPPPPPMKPSPILPAKEETVHVKLLPPKKPLPSCPRPQQLNPPSKPLPVTPAHKEPLLVMTPATHNKAPITNSATQLKGPRRPIQGGKVQAAAAAFLQRK

Target ORF information:

RefSeq Version	NM_001040013.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ADAM metallopeptidase domain 33 (adam33), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040013.2

ORF Insert Sequence:

ATGGGGACGG AGGGTTGGCT GCACACATGG CTGGGACTGG GGGCTGTGTT TGTGGGGCTG 
CTCCTGGCTC CGGTGGCGGC ACTGGGGGCC CACCAAGGAG AGCGGGTGAC TGCCCTCTGG 
CTACAGAATG AAAGAGCTAA GAGATCAGTA GAGCTGCTGG ATGAGGGGAC TCCAGACCGG 
GGAGAAATAC TGGTGACATC AGAAGGAAGG CGGTTGATCC TGAAAGTGGA GAGAAATCAC 
CGCCTGTTTG CGCCCGGCTA CACTGAGACA CATTACACTG ATGGGCAGAT GGTCACATTG 
TCTCCCAATC ACACGGAGCA TTGCTATTAC CATGGACAAG TGCAAGGCTA TGATGACTCC 
TCTGTCGCTC TCACCACCTG CTCAGGAATC AGTGGTTTGA TTGTGCTGAG AACTAATGAC 
AGCTACTACC TGAGTCCCCT GGAGGTATCA GGTAAAGAAA CTCATTCACT GGTCCGCACT 
GAACACCTGC CGATCAAAGG TGGGAGCTGT GGGCATGAAG GACATTCAGG AAGCACCACC 
TCTTACTTCA AGGAGTTTAC AGCACCTCCT GGCCATCACA GGGTAAGGAG GAACGTCTGG 
AGGTCCCAGA AATACATGGA GCTATTTATT GTTGCAGATT ACAGCATGTT TATGAAACAG 
AATCGGAATC TAGGCAGCAC GAAGCAACGT GTCCTTGAAA TTGCCAATTA TGTAGACAAG 
TTCTACATGT CCATGAACAT CAAGGTGGCT CTGATAGGGC TGGAAGTCTG GACAGAGAGG 
GATCAATGTG ATGTGAATGA TGATGCCAAC GACAGCCTGA GATCGTTCCT ACAGTGGAAG 
CAAAAGTTGA GGTCCCGGAA GAAGCACGAT AACGCGCAGC TAATCACAGG GGTGACGTTT 
AAAGGAACAA CCATTGGGAT GGCACCACTG GAAGGCATGT GCACTGCTGA GAACTCAGGG 
GGAGTCAGTA TGGATCACTC AGAAAATGCA ATTGGAGCTG CTGCAACAAT GGCCCATGAA 
ATTGGACACA ATTTTGGAAT GAGCCACGAC AATGACGGCT GCTGTGTGGA GGCCACTCCG 
GAACAAGGAG GCTGTATCAT GGCGGCTTCC ACTGGGCATC CCTTCCCTCG CAAGTTCAGC 
CGGTGCAGTC AGCAGCAGCT TATGAGTTAT TTCAGGAAGG GGGGTGGAAT GTGCCTGTTC 
AACATGCCAA ACACCAATGA CCTCGTGATG GGGAAAAAGT GTGGAAATGG GTTCCTGGAG 
GAAGGAGAGC AGTGTGACTG TGGGGAGCCA GAGGAATGCA CTAATCCCTG CTGCAATGCA 
AATAACTGCA CTTTAAAGGC CGGCGCCAAG TGCGCTCATG GCGAATGTTG CCAGGATTGT 
AGGCTGAAAT CTGCAGGGAC TCAGTGCCGG GAAATGGCTG GATCCTGTGA CCTTCCAGAA 
TTCTGCACGG GGGATGCCCC TTTCTGTCCC TCCAATGTGT ATATGCTGGA TGGGTCCTCA 
TGTGCTTTTG GGGAGGCATA CTGTTATAAT GGCATGTGTC TCACTCACCA GCAGCAGTGT 
ATCCATCTTT GGGGTTCAGG AGCTGTGGTT GCCCCTGACT TCTGCTTCCA AGATGTGAAC 
AAAGCAGGAG ACCAGTATGG AAACTGCGGC AAGAATGGAA GAGGACAATT TGTGAAGTGT 
ACATCCCGAG ATGCTAAGTG TGGAAAAATC CAATGCCAGA CGAGCTCAGA AAAGCCCAGG 
GACCCCAGCA TGGTGAAGGT GGACAACACC ATTATCATTA ATGGCTACAA GATGAAGTGT 
CAGGGGGTAC ATGCCTACTC CATGCAAGAA GAGGAAGGTG ACCCGGGGCT CGTAATGACT 
GGGACCAAGT GTGGAGACGG CATGGTATGC AAGGATCGTC GCTGTCAGAA TGCCTCCTTC 
TTTGAGCTGG ACCAGTGCGT GTCTAAGTGT AATGGACATG GGGTCTGTAA CAGCAACAGA 
AACTGCCATT GTCATTCAGG CTGGGCCCCT CCTTACTGTG ACAAACCTGG GCCAGGTGGC 
AGTCAAGACA GTGGCCCTGC ACCTAATGAC ATTCCTGTGG GTGTCACTAT TTTCTTGGTT 
ATCCTGTTCC TGGTTCTGCT GCTGGGTTTG GCTATTGCGA TGGTCTACTG GTATCGAAAA 
CCAGGTTCTC TCCTGAACAA GTGGCTGAAG AAGAGCAAAG CAAAATGCAG CTTATGCAAA 
GCCACCCAAC CAAAGGCAAA CAGGGCGTAT TCAAGCAAGA TCTTTACTAT GCGCGATATA 
TCCTATCCAG TTAAATCCGA CAGCAAACAA ACTCACTCAC GGGACATATT CCAAGGAAAA 
ACAACTGCTG CTCAGAACAG TTCTCATCCT GTTAACGTTG TGCGTCCTCT TCGTCCGGCT 
CCCAGCCCAG TGATCCAACA TGGGAAGCCT CTTAGGCCTC CTCCTCCTCC AATGAAACCG 
TCGCCCATTC TGCCTGCAAA AGAAGAAACC GTTCATGTAA AACTGCTACC CCCCAAGAAA 
CCTCTACCCT CTTGCCCTAG GCCGCAGCAG CTGAACCCAC CTTCCAAGCC ACTGCCGGTG 
ACTCCTGCTC ACAAAGAGCC ACTGTTGGTT ATGACACCGG CCACACATAA CAAGGCTCCC 
ATCACCAACT CTGCTACCCA GCTTAAAGGG CCACGCAGGC CAATACAAGG GGGCAAGGTC 
CAAGCTGCTG CTGCTGCCTT TCTTCAGAGA AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

adam33 ( XM_012963957.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819411.1 Xenopus tropicalis ADAM metallopeptidase domain 33 (adam33), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa28447
Clone ID Related Accession (Same CDS sequence)	XM_012963957.2
Accession Version	XM_012963957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ADAM metallopeptidase domain 33 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGACGG AGGGTTGGCT GCACACATGG CTGGGACTGG GGACTGTGTT TGTGGGGCTG 
CTCCTGGCTC CGGTGGCGGC ACTGGGGGCC CACCAAGGAG AGCGGGTGAC TGCCCTCTGG 
CTACAGAATG AAAGAGCTAA GAGATCAGTA GAGCTGCTGG ATAAGGTCAG TGGGACTCCA 
GACCGGGGAG AAATACTGGT GACATCAGAA GGAAGGCGGT TGATCCTGAA AGTGGAGAGA 
AATCACCGCC TGTTTGCGCC CGGCTACACT GAGACACATT ACACTGATGG GCAGATGGTC 
ACATTGTCTC CCAATCACAC GGAGCATTGC TATTACCATG GACAAGTGCA AGGCTATGAT 
GACTCCTCTG TCGCTCTCAC CACCTGCTCA GGAATCAGTG GTTTGATTGT GCTGAGAACT 
AATGACAGCT ACTACCTGAG TCCCCTGGAG GTATCAGGTA AAGAAACTCA TTCACTGGTC 
CGCACTGAAC ACCTGCCGAT CAAAGGTGGG AGCTGTGGGC ATGAAGGACA TTCAGGAAGC 
ACCACCTCTT ACTTCAAGGA GTTTACAGCA CCTCCTGGCC ATCACAGGGT AAGGAGGAAC 
GTCTGGAGGT CTCAGAAATA CATGGAGCTA TTTATTGTTG CAGATTACAG CATGTTTATG 
AAACAGAATC GGAATCTAGG CAGCACGAAG CAACGTGTCC TTGAAATTGC CAATTATGTA 
GACAAGTTCT ACATGTCCAT GAACATCAAG GTGGCTCTGA TAGGGCTGGA AGTCTGGACA 
GAGAGGGATC AATGTGATGT GAATGATGAT GCCAACGACA GCCTGAGATC GTTCCTACAG 
TGGAAGCAAA AGTTGAGGTC CCGGAAGAAG CACGATAACG CGCAGCTAAT CACAGGGGTG 
ACGTTTAAAG GAACAACCAT TGGGATGGCA CCACTGGAAG GCATGTGCAC TGCTGAGAAC 
TCAGGGGGAG TCAGTATGGA TCACTCAGAA AATGCAATTG GAGCTGCTGC AACAATGGCC 
CATGAAATTG GACACAATTT TGGAATGAGC CACGACAATG ACGGCTGCTG TGTGGAGGCC 
ACTCCGGAAC AAGGAGGCTG TATCATGGCG GCTTCCACTG GGCATCCCTT CCCTCGCAAG 
TTCAGCCGGT GCAGTCAGCA GCAGCTTATG AGTTATTTCA GGAAGGGGGG TGGAATGTGC 
CTGTTCAACA TGCCAAACAC CAATGACCTC GTGATGGGGA AAAAGTGTGG AAATGGGTTC 
CTGGAGGAAG GAGAGCAGTG TGACTGTGGG GAGCCAGAGG AATGCACTAA TCCCTGCTGC 
AATGCAAATA ACTGCACTTT AAAGGCCGGC GCCAAGTGCG CTCATGGCGA ATGTTGCCAG 
GATTGTAGGC TGAAATCTGC AGGGACTCAG TGCCGGGAAA TGGCTGGATC CTGTGACCTT 
CCAGAATTCT GCACGGGGGA TGCCCCTTTC TGTCCCTCCA ATGTGTATAT GCTGGATGGG 
TCCTCATGTG CTTTTGGGGA GGCATACTGT TATAATGGCA TGTGTCTCAC TCACCAGCAG 
CAGTGTATCC ATCTTTGGGG TTCAGGAGCT GTGGTTGCCC CTGACTTCTG CTTCCAAGAT 
GTGAACAAAG CAGGAGACCA GTATGGAAAC TGCGGCAAGA ATGGAAGAGG ACAATTTGTG 
AAGTGTACAT CCCGAGATGC TAAGTGTGGA AAAATCCAAT GCCAGACGAG CTCAGAAAAG 
CCCAGGGACC CCAGCATGGT GAAGGTGGAC AACACCATTA TCATTAATGG CTACAAGATG 
AAGTGTCAGG GGGTACATGC CTACTCCATG CAAGAAGAGG AAGGTGACCC GGGGCTCGTA 
ATGACTGGGA CCAAGTGTGG AGACGGCATG GTATGCAAGG ATCGTCGCTG TCAGAATGCC 
TCCTTCTTTG AGCTGGACCA GTGCGTGTCT AAGTGTAATG GACATGGGGT CTGTAACAGC 
AACAGAAACT GCCATTGTCA TTCAGGCTGG GCCCCTCCTT ACTGTGACAA ACCTGGGCCA 
GGTGGCAGTC AAGACAGTGG CCCTGCACCT AATGACATTC CTGTGGGTGT CACTATTTTC 
TTGGTTATCC TGTTCCTGGT TCTGCTGCTG GGTTTGGCTA TTGCGATGGT CTACTGGTAT 
CGAAAACCAG GTTCTCTCCT GAACAAGTGG CTGAAGAAGA GCAAAGCAAA ATGCAGCTTA 
TGCAAAGCCA CCCAACCAAA GGCAAACAGA GCGTATTCAA GCAAGATCTT TACTATGCGC 
GATATATCCT ATCCAGTTAA ATCCGACAGC AAACAAACTC ACTCACGGGA CATATTCCAA 
GGAAAAACAA CTGCTGCTCA GAACAGTTCT CATCCTGTTA ACGTTGTGCG TCCTCTTCGT 
CCGGCTCCCA GCCCAGTGAT CCAACATGGG AAGCCTCTTA GGCCTCCTCC TCCTCCAATG 
AAACCGTCGC CCATTCTGCC TGCAAAAGAA GAAACCGTTC ATGTAAAACT GCTACCCCCC 
AAGAAACCTC TACCCTCTTG CCCTAGGCCG CAGCAGCTGA ACCCACCTTC CAAGCCACTG 
CCGGTGACTC CTGCTCACAA AGAGCCACTG TTGGTTATGA CACCGGCCAC ACATAACAAG 
GCTCCCATCA CCAACTCTGC TACCCAGCTT AAAGGGCCAC GCAGGCCAAT ACAAGGGGGC 
AAGGTCCAAG CTGCTGCTGC TGCCTTTCTT CAGAGAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819411.1
CDS	33..2774
Translation	MGTEGWLHTWLGLGTVFVGLLLAPVAALGAHQGERVTALWLQNERAKRSVELLDKVSGTPDRGEILVTSEGRRLILKVERNHRLFAPGYTETHYTDGQMVTLSPNHTEHCYYHGQVQGYDDSSVALTTCSGISGLIVLRTNDSYYLSPLEVSGKETHSLVRTEHLPIKGGSCGHEGHSGSTTSYFKEFTAPPGHHRVRRNVWRSQKYMELFIVADYSMFMKQNRNLGSTKQRVLEIANYVDKFYMSMNIKVALIGLEVWTERDQCDVNDDANDSLRSFLQWKQKLRSRKKHDNAQLITGVTFKGTTIGMAPLEGMCTAENSGGVSMDHSENAIGAAATMAHEIGHNFGMSHDNDGCCVEATPEQGGCIMAASTGHPFPRKFSRCSQQQLMSYFRKGGGMCLFNMPNTNDLVMGKKCGNGFLEEGEQCDCGEPEECTNPCCNANNCTLKAGAKCAHGECCQDCRLKSAGTQCREMAGSCDLPEFCTGDAPFCPSNVYMLDGSSCAFGEAYCYNGMCLTHQQQCIHLWGSGAVVAPDFCFQDVNKAGDQYGNCGKNGRGQFVKCTSRDAKCGKIQCQTSSEKPRDPSMVKVDNTIIINGYKMKCQGVHAYSMQEEEGDPGLVMTGTKCGDGMVCKDRRCQNASFFELDQCVSKCNGHGVCNSNRNCHCHSGWAPPYCDKPGPGGSQDSGPAPNDIPVGVTIFLVILFLVLLLGLAIAMVYWYRKPGSLLNKWLKKSKAKCSLCKATQPKANRAYSSKIFTMRDISYPVKSDSKQTHSRDIFQGKTTAAQNSSHPVNVVRPLRPAPSPVIQHGKPLRPPPPPMKPSPILPAKEETVHVKLLPPKKPLPSCPRPQQLNPPSKPLPVTPAHKEPLLVMTPATHNKAPITNSATQLKGPRRPIQGGKVQAAAAAFLQRK

Target ORF information:

RefSeq Version	XM_012963957.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ADAM metallopeptidase domain 33 (adam33), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963957.2

ORF Insert Sequence:

ATGGGGACGG AGGGTTGGCT GCACACATGG CTGGGACTGG GGACTGTGTT TGTGGGGCTG 
CTCCTGGCTC CGGTGGCGGC ACTGGGGGCC CACCAAGGAG AGCGGGTGAC TGCCCTCTGG 
CTACAGAATG AAAGAGCTAA GAGATCAGTA GAGCTGCTGG ATAAGGTCAG TGGGACTCCA 
GACCGGGGAG AAATACTGGT GACATCAGAA GGAAGGCGGT TGATCCTGAA AGTGGAGAGA 
AATCACCGCC TGTTTGCGCC CGGCTACACT GAGACACATT ACACTGATGG GCAGATGGTC 
ACATTGTCTC CCAATCACAC GGAGCATTGC TATTACCATG GACAAGTGCA AGGCTATGAT 
GACTCCTCTG TCGCTCTCAC CACCTGCTCA GGAATCAGTG GTTTGATTGT GCTGAGAACT 
AATGACAGCT ACTACCTGAG TCCCCTGGAG GTATCAGGTA AAGAAACTCA TTCACTGGTC 
CGCACTGAAC ACCTGCCGAT CAAAGGTGGG AGCTGTGGGC ATGAAGGACA TTCAGGAAGC 
ACCACCTCTT ACTTCAAGGA GTTTACAGCA CCTCCTGGCC ATCACAGGGT AAGGAGGAAC 
GTCTGGAGGT CTCAGAAATA CATGGAGCTA TTTATTGTTG CAGATTACAG CATGTTTATG 
AAACAGAATC GGAATCTAGG CAGCACGAAG CAACGTGTCC TTGAAATTGC CAATTATGTA 
GACAAGTTCT ACATGTCCAT GAACATCAAG GTGGCTCTGA TAGGGCTGGA AGTCTGGACA 
GAGAGGGATC AATGTGATGT GAATGATGAT GCCAACGACA GCCTGAGATC GTTCCTACAG 
TGGAAGCAAA AGTTGAGGTC CCGGAAGAAG CACGATAACG CGCAGCTAAT CACAGGGGTG 
ACGTTTAAAG GAACAACCAT TGGGATGGCA CCACTGGAAG GCATGTGCAC TGCTGAGAAC 
TCAGGGGGAG TCAGTATGGA TCACTCAGAA AATGCAATTG GAGCTGCTGC AACAATGGCC 
CATGAAATTG GACACAATTT TGGAATGAGC CACGACAATG ACGGCTGCTG TGTGGAGGCC 
ACTCCGGAAC AAGGAGGCTG TATCATGGCG GCTTCCACTG GGCATCCCTT CCCTCGCAAG 
TTCAGCCGGT GCAGTCAGCA GCAGCTTATG AGTTATTTCA GGAAGGGGGG TGGAATGTGC 
CTGTTCAACA TGCCAAACAC CAATGACCTC GTGATGGGGA AAAAGTGTGG AAATGGGTTC 
CTGGAGGAAG GAGAGCAGTG TGACTGTGGG GAGCCAGAGG AATGCACTAA TCCCTGCTGC 
AATGCAAATA ACTGCACTTT AAAGGCCGGC GCCAAGTGCG CTCATGGCGA ATGTTGCCAG 
GATTGTAGGC TGAAATCTGC AGGGACTCAG TGCCGGGAAA TGGCTGGATC CTGTGACCTT 
CCAGAATTCT GCACGGGGGA TGCCCCTTTC TGTCCCTCCA ATGTGTATAT GCTGGATGGG 
TCCTCATGTG CTTTTGGGGA GGCATACTGT TATAATGGCA TGTGTCTCAC TCACCAGCAG 
CAGTGTATCC ATCTTTGGGG TTCAGGAGCT GTGGTTGCCC CTGACTTCTG CTTCCAAGAT 
GTGAACAAAG CAGGAGACCA GTATGGAAAC TGCGGCAAGA ATGGAAGAGG ACAATTTGTG 
AAGTGTACAT CCCGAGATGC TAAGTGTGGA AAAATCCAAT GCCAGACGAG CTCAGAAAAG 
CCCAGGGACC CCAGCATGGT GAAGGTGGAC AACACCATTA TCATTAATGG CTACAAGATG 
AAGTGTCAGG GGGTACATGC CTACTCCATG CAAGAAGAGG AAGGTGACCC GGGGCTCGTA 
ATGACTGGGA CCAAGTGTGG AGACGGCATG GTATGCAAGG ATCGTCGCTG TCAGAATGCC 
TCCTTCTTTG AGCTGGACCA GTGCGTGTCT AAGTGTAATG GACATGGGGT CTGTAACAGC 
AACAGAAACT GCCATTGTCA TTCAGGCTGG GCCCCTCCTT ACTGTGACAA ACCTGGGCCA 
GGTGGCAGTC AAGACAGTGG CCCTGCACCT AATGACATTC CTGTGGGTGT CACTATTTTC 
TTGGTTATCC TGTTCCTGGT TCTGCTGCTG GGTTTGGCTA TTGCGATGGT CTACTGGTAT 
CGAAAACCAG GTTCTCTCCT GAACAAGTGG CTGAAGAAGA GCAAAGCAAA ATGCAGCTTA 
TGCAAAGCCA CCCAACCAAA GGCAAACAGA GCGTATTCAA GCAAGATCTT TACTATGCGC 
GATATATCCT ATCCAGTTAA ATCCGACAGC AAACAAACTC ACTCACGGGA CATATTCCAA 
GGAAAAACAA CTGCTGCTCA GAACAGTTCT CATCCTGTTA ACGTTGTGCG TCCTCTTCGT 
CCGGCTCCCA GCCCAGTGAT CCAACATGGG AAGCCTCTTA GGCCTCCTCC TCCTCCAATG 
AAACCGTCGC CCATTCTGCC TGCAAAAGAA GAAACCGTTC ATGTAAAACT GCTACCCCCC 
AAGAAACCTC TACCCTCTTG CCCTAGGCCG CAGCAGCTGA ACCCACCTTC CAAGCCACTG 
CCGGTGACTC CTGCTCACAA AGAGCCACTG TTGGTTATGA CACCGGCCAC ACATAACAAG 
GCTCCCATCA CCAACTCTGC TACCCAGCTT AAAGGGCCAC GCAGGCCAAT ACAAGGGGGC 
AAGGTCCAAG CTGCTGCTGC TGCCTTTCTT CAGAGAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

adam33 ( XM_012963962.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819416.1 Xenopus tropicalis ADAM metallopeptidase domain 33 (adam33), transcript variant X2, mRNA.

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CloneID	OXa28448
Clone ID Related Accession (Same CDS sequence)	XM_012963962.2
Accession Version	XM_012963962.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ADAM metallopeptidase domain 33 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963962.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGGGG CCCACCAAGG AGAGCGGGTG ACTGCCCTCT GGCTACAGAA TGAAAGAGCT 
AAGAGATCAG TAGAGCTGCT GGATAAGGTC AGTGGGACTC CAGACCGGGG AGAAATACTG 
GTGACATCAG AAGGAAGGCG GTTGATCCTG AAAGTGGAGA GAAATCACCG CCTGTTTGCG 
CCCGGCTACA CTGAGACACA TTACACTGAT GGGCAGATGG TCACATTGTC TCCCAATCAC 
ACGGAGCATT GCTATTACCA TGGACAAGTG CAAGGCTATG ATGACTCCTC TGTCGCTCTC 
ACCACCTGCT CAGGAATCAG TGGTTTGATT GTGCTGAGAA CTAATGACAG CTACTACCTG 
AGTCCCCTGG AGGTATCAGG TAAAGAAACT CATTCACTGG TCCGCACTGA ACACCTGCCG 
ATCAAAGGTG GGAGCTGTGG GCATGAAGGA CATTCAGGAA GCACCACCTC TTACTTCAAG 
GAGTTTACAG CACCTCCTGG CCATCACAGG GTAAGGAGGA ACGTCTGGAG GTCTCAGAAA 
TACATGGAGC TATTTATTGT TGCAGATTAC AGCATGTTTA TGAAACAGAA TCGGAATCTA 
GGCAGCACGA AGCAACGTGT CCTTGAAATT GCCAATTATG TAGACAAGTT CTACATGTCC 
ATGAACATCA AGGTGGCTCT GATAGGGCTG GAAGTCTGGA CAGAGAGGGA TCAATGTGAT 
GTGAATGATG ATGCCAACGA CAGCCTGAGA TCGTTCCTAC AGTGGAAGCA AAAGTTGAGG 
TCCCGGAAGA AGCACGATAA CGCGCAGCTA ATCACAGGGG TGACGTTTAA AGGAACAACC 
ATTGGGATGG CACCACTGGA AGGCATGTGC ACTGCTGAGA ACTCAGGGGG AGTCAGTATG 
GATCACTCAG AAAATGCAAT TGGAGCTGCT GCAACAATGG CCCATGAAAT TGGACACAAT 
TTTGGAATGA GCCACGACAA TGACGGCTGC TGTGTGGAGG CCACTCCGGA ACAAGGAGGC 
TGTATCATGG CGGCTTCCAC TGGGCATCCC TTCCCTCGCA AGTTCAGCCG GTGCAGTCAG 
CAGCAGCTTA TGAGTTATTT CAGGAAGGGG GGTGGAATGT GCCTGTTCAA CATGCCAAAC 
ACCAATGACC TCGTGATGGG GAAAAAGTGT GGAAATGGGT TCCTGGAGGA AGGAGAGCAG 
TGTGACTGTG GGGAGCCAGA GGAATGCACT AATCCCTGCT GCAATGCAAA TAACTGCACT 
TTAAAGGCCG GCGCCAAGTG CGCTCATGGC GAATGTTGCC AGGATTGTAG GCTGAAATCT 
GCAGGGACTC AGTGCCGGGA AATGGCTGGA TCCTGTGACC TTCCAGAATT CTGCACGGGG 
GATGCCCCTT TCTGTCCCTC CAATGTGTAT ATGCTGGATG GGTCCTCATG TGCTTTTGGG 
GAGGCATACT GTTATAATGG CATGTGTCTC ACTCACCAGC AGCAGTGTAT CCATCTTTGG 
GGTTCAGGAG CTGTGGTTGC CCCTGACTTC TGCTTCCAAG ATGTGAACAA AGCAGGAGAC 
CAGTATGGAA ACTGCGGCAA GAATGGAAGA GGACAATTTG TGAAGTGTAC ATCCCGAGAT 
GCTAAGTGTG GAAAAATCCA ATGCCAGACG AGCTCAGAAA AGCCCAGGGA CCCCAGCATG 
GTGAAGGTGG ACAACACCAT TATCATTAAT GGCTACAAGA TGAAGTGTCA GGGGGTACAT 
GCCTACTCCA TGCAAGAAGA GGAAGGTGAC CCGGGGCTCG TAATGACTGG GACCAAGTGT 
GGAGACGGCA TGGTATGCAA GGATCGTCGC TGTCAGAATG CCTCCTTCTT TGAGCTGGAC 
CAGTGCGTGT CTAAGTGTAA TGGACATGGG GTCTGTAACA GCAACAGAAA CTGCCATTGT 
CATTCAGGCT GGGCCCCTCC TTACTGTGAC AAACCTGGGC CAGGTGGCAG TCAAGACAGT 
GGCCCTGCAC CTAATGACAT TCCTGTGGGT GTCACTATTT TCTTGGTTAT CCTGTTCCTG 
GTTCTGCTGC TGGGTTTGGC TATTGCGATG GTCTACTGGT ATCGAAAACC AGGTTCTCTC 
CTGAACAAGT GGCTGAAGAA GAGCAAAGCA AAATGCAGCT TATGCAAAGC CACCCAACCA 
AAGGCAAACA GAGCGTATTC AAGCAAGATC TTTACTATGC GCGATATATC CTATCCAGTT 
AAATCCGACA GCAAACAAAC TCACTCACGG GACATATTCC AAGGAAAAAC AACTGCTGCT 
CAGAACAGTT CTCATCCTGT TAACGTTGTG CGTCCTCTTC GTCCGGCTCC CAGCCCAGTG 
ATCCAACATG GGAAGCCTCT TAGGCCTCCT CCTCCTCCAA TGAAACCGTC GCCCATTCTG 
CCTGCAAAAG AAGAAACCGT TCATGTAAAA CTGCTACCCC CCAAGAAACC TCTACCCTCT 
TGCCCTAGGC CGCAGCAGCT GAACCCACCT TCCAAGCCAC TGCCGGTGAC TCCTGCTCAC 
AAAGAGCCAC TGTTGGTTAT GACACCGGCC ACACATAACA AGGCTCCCAT CACCAACTCT 
GCTACCCAGC TTAAAGGGCC ACGCAGGCCA ATACAAGGGG GCAAGGTCCA AGCTGCTGCT 
GCTGCCTTTC TTCAGAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819416.1
CDS	135..2798
Translation	MTGAHQGERVTALWLQNERAKRSVELLDKVSGTPDRGEILVTSEGRRLILKVERNHRLFAPGYTETHYTDGQMVTLSPNHTEHCYYHGQVQGYDDSSVALTTCSGISGLIVLRTNDSYYLSPLEVSGKETHSLVRTEHLPIKGGSCGHEGHSGSTTSYFKEFTAPPGHHRVRRNVWRSQKYMELFIVADYSMFMKQNRNLGSTKQRVLEIANYVDKFYMSMNIKVALIGLEVWTERDQCDVNDDANDSLRSFLQWKQKLRSRKKHDNAQLITGVTFKGTTIGMAPLEGMCTAENSGGVSMDHSENAIGAAATMAHEIGHNFGMSHDNDGCCVEATPEQGGCIMAASTGHPFPRKFSRCSQQQLMSYFRKGGGMCLFNMPNTNDLVMGKKCGNGFLEEGEQCDCGEPEECTNPCCNANNCTLKAGAKCAHGECCQDCRLKSAGTQCREMAGSCDLPEFCTGDAPFCPSNVYMLDGSSCAFGEAYCYNGMCLTHQQQCIHLWGSGAVVAPDFCFQDVNKAGDQYGNCGKNGRGQFVKCTSRDAKCGKIQCQTSSEKPRDPSMVKVDNTIIINGYKMKCQGVHAYSMQEEEGDPGLVMTGTKCGDGMVCKDRRCQNASFFELDQCVSKCNGHGVCNSNRNCHCHSGWAPPYCDKPGPGGSQDSGPAPNDIPVGVTIFLVILFLVLLLGLAIAMVYWYRKPGSLLNKWLKKSKAKCSLCKATQPKANRAYSSKIFTMRDISYPVKSDSKQTHSRDIFQGKTTAAQNSSHPVNVVRPLRPAPSPVIQHGKPLRPPPPPMKPSPILPAKEETVHVKLLPPKKPLPSCPRPQQLNPPSKPLPVTPAHKEPLLVMTPATHNKAPITNSATQLKGPRRPIQGGKVQAAAAAFLQRK

Target ORF information:

RefSeq Version	XM_012963962.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ADAM metallopeptidase domain 33 (adam33), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963962.2

ORF Insert Sequence:

ATGACAGGGG CCCACCAAGG AGAGCGGGTG ACTGCCCTCT GGCTACAGAA TGAAAGAGCT 
AAGAGATCAG TAGAGCTGCT GGATAAGGTC AGTGGGACTC CAGACCGGGG AGAAATACTG 
GTGACATCAG AAGGAAGGCG GTTGATCCTG AAAGTGGAGA GAAATCACCG CCTGTTTGCG 
CCCGGCTACA CTGAGACACA TTACACTGAT GGGCAGATGG TCACATTGTC TCCCAATCAC 
ACGGAGCATT GCTATTACCA TGGACAAGTG CAAGGCTATG ATGACTCCTC TGTCGCTCTC 
ACCACCTGCT CAGGAATCAG TGGTTTGATT GTGCTGAGAA CTAATGACAG CTACTACCTG 
AGTCCCCTGG AGGTATCAGG TAAAGAAACT CATTCACTGG TCCGCACTGA ACACCTGCCG 
ATCAAAGGTG GGAGCTGTGG GCATGAAGGA CATTCAGGAA GCACCACCTC TTACTTCAAG 
GAGTTTACAG CACCTCCTGG CCATCACAGG GTAAGGAGGA ACGTCTGGAG GTCTCAGAAA 
TACATGGAGC TATTTATTGT TGCAGATTAC AGCATGTTTA TGAAACAGAA TCGGAATCTA 
GGCAGCACGA AGCAACGTGT CCTTGAAATT GCCAATTATG TAGACAAGTT CTACATGTCC 
ATGAACATCA AGGTGGCTCT GATAGGGCTG GAAGTCTGGA CAGAGAGGGA TCAATGTGAT 
GTGAATGATG ATGCCAACGA CAGCCTGAGA TCGTTCCTAC AGTGGAAGCA AAAGTTGAGG 
TCCCGGAAGA AGCACGATAA CGCGCAGCTA ATCACAGGGG TGACGTTTAA AGGAACAACC 
ATTGGGATGG CACCACTGGA AGGCATGTGC ACTGCTGAGA ACTCAGGGGG AGTCAGTATG 
GATCACTCAG AAAATGCAAT TGGAGCTGCT GCAACAATGG CCCATGAAAT TGGACACAAT 
TTTGGAATGA GCCACGACAA TGACGGCTGC TGTGTGGAGG CCACTCCGGA ACAAGGAGGC 
TGTATCATGG CGGCTTCCAC TGGGCATCCC TTCCCTCGCA AGTTCAGCCG GTGCAGTCAG 
CAGCAGCTTA TGAGTTATTT CAGGAAGGGG GGTGGAATGT GCCTGTTCAA CATGCCAAAC 
ACCAATGACC TCGTGATGGG GAAAAAGTGT GGAAATGGGT TCCTGGAGGA AGGAGAGCAG 
TGTGACTGTG GGGAGCCAGA GGAATGCACT AATCCCTGCT GCAATGCAAA TAACTGCACT 
TTAAAGGCCG GCGCCAAGTG CGCTCATGGC GAATGTTGCC AGGATTGTAG GCTGAAATCT 
GCAGGGACTC AGTGCCGGGA AATGGCTGGA TCCTGTGACC TTCCAGAATT CTGCACGGGG 
GATGCCCCTT TCTGTCCCTC CAATGTGTAT ATGCTGGATG GGTCCTCATG TGCTTTTGGG 
GAGGCATACT GTTATAATGG CATGTGTCTC ACTCACCAGC AGCAGTGTAT CCATCTTTGG 
GGTTCAGGAG CTGTGGTTGC CCCTGACTTC TGCTTCCAAG ATGTGAACAA AGCAGGAGAC 
CAGTATGGAA ACTGCGGCAA GAATGGAAGA GGACAATTTG TGAAGTGTAC ATCCCGAGAT 
GCTAAGTGTG GAAAAATCCA ATGCCAGACG AGCTCAGAAA AGCCCAGGGA CCCCAGCATG 
GTGAAGGTGG ACAACACCAT TATCATTAAT GGCTACAAGA TGAAGTGTCA GGGGGTACAT 
GCCTACTCCA TGCAAGAAGA GGAAGGTGAC CCGGGGCTCG TAATGACTGG GACCAAGTGT 
GGAGACGGCA TGGTATGCAA GGATCGTCGC TGTCAGAATG CCTCCTTCTT TGAGCTGGAC 
CAGTGCGTGT CTAAGTGTAA TGGACATGGG GTCTGTAACA GCAACAGAAA CTGCCATTGT 
CATTCAGGCT GGGCCCCTCC TTACTGTGAC AAACCTGGGC CAGGTGGCAG TCAAGACAGT 
GGCCCTGCAC CTAATGACAT TCCTGTGGGT GTCACTATTT TCTTGGTTAT CCTGTTCCTG 
GTTCTGCTGC TGGGTTTGGC TATTGCGATG GTCTACTGGT ATCGAAAACC AGGTTCTCTC 
CTGAACAAGT GGCTGAAGAA GAGCAAAGCA AAATGCAGCT TATGCAAAGC CACCCAACCA 
AAGGCAAACA GAGCGTATTC AAGCAAGATC TTTACTATGC GCGATATATC CTATCCAGTT 
AAATCCGACA GCAAACAAAC TCACTCACGG GACATATTCC AAGGAAAAAC AACTGCTGCT 
CAGAACAGTT CTCATCCTGT TAACGTTGTG CGTCCTCTTC GTCCGGCTCC CAGCCCAGTG 
ATCCAACATG GGAAGCCTCT TAGGCCTCCT CCTCCTCCAA TGAAACCGTC GCCCATTCTG 
CCTGCAAAAG AAGAAACCGT TCATGTAAAA CTGCTACCCC CCAAGAAACC TCTACCCTCT 
TGCCCTAGGC CGCAGCAGCT GAACCCACCT TCCAAGCCAC TGCCGGTGAC TCCTGCTCAC 
AAAGAGCCAC TGTTGGTTAT GACACCGGCC ACACATAACA AGGCTCCCAT CACCAACTCT 
GCTACCCAGC TTAAAGGGCC ACGCAGGCCA ATACAAGGGG GCAAGGTCCA AGCTGCTGCT 
GCTGCCTTTC TTCAGAGAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

adam33 ( XM_012963957.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819411.2 Xenopus tropicalis ADAM metallopeptidase domain 33 (adam33), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa47959
Clone ID Related Accession (Same CDS sequence)	XM_012963957.3
Accession Version	XM_012963957.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ADAM metallopeptidase domain 33 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963957.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGACGG AGGGTTGGCT GCACACATGG CTGGGACTGG GGGCTGTGTT TGTGGGGCTG 
CTCCTGGCTC CGGTGGCGGC ACTGGGGGCC CACCAAGGAG AGCGGGTGAC TGCCCTCTGG 
CTACAGAATG AAAGAGCTAA GAGATCAGTA GAGCTGCTGG ATAAGGTCAG TGGGACTCCA 
GACCGGGGAG AAATACTGGT GACATCAGAA GGAAGGCGGT TGATCCTGAA AGTGGAGAGA 
AATCACCGCC TGTTTGCGCC CGGCTACACT GAGACACATT ACACTGATGG GCAGATGGTC 
ACATTGTCTC CCAATCACAC GGAGCATTGC TATTACCATG GACAAGTGCA AGGCTATGAT 
GACTCCTCTG TCGCTCTCAC CACCTGCTCA GGAATCAGTG GTTTGATTGT GCTGAGAACT 
AATGACAGCT ACTACCTGAG TCCCCTGGAG GTATCAGGTA AAGAAACTCA TTCACTGGTC 
CGCACTGAAC ACCTGCCGAT CAAAGGTGGG AGCTGTGGGC ATGAAGGACA TTCAGGAAGC 
ACCACCTCTT ACTTCAAGGA GTTTACAGCA CCTCCTGGCC ATCACAGGGT AAGGAGGAAC 
GTCTGGAGGT CTCAGAAATA CATGGAGCTA TTTATTGTTG CAGATTACAG CATGTTTATG 
AAACAGAATC GGAATCTAGG CAGCACGAAG CAACGTGTCC TTGAAATTGC CAATTATGTA 
GACAAGTTCT ACATGTCCAT GAACATCAAG GTGGCTCTGA TAGGGCTGGA AGTCTGGACA 
GAGAGGGATC AATGTGATGT GAATGATGAT GCCAACGACA GCCTGAGATC GTTCCTACAG 
TGGAAGCAAA AGTTGAGGTC CCGGAAGAAG CACGATAACG CGCAGCTAAT CACAGGGGTG 
ACGTTTAAAG GAACAACCAT TGGGATGGCA CCACTGGAAG GCATGTGCAC TGCTGAGAAC 
TCAGGGGGAG TCAGTATGGA TCACTCAGAA AATGCAATTG GAGCTGCTGC AACAATGGCC 
CATGAAATTG GACACAATTT TGGAATGAGC CACGACAATG ACGGCTGCTG TGTGGAGGCC 
ACTCCGGAAC AAGGAGGCTG TATCATGGCG GCTTCCACTG GGCATCCCTT CCCTCGCAAG 
TTCAGCCGGT GCAGTCAGCA GCAGCTTATG AGTTATTTCA GGAAGGGGGG TGGAATGTGC 
CTGTTCAACA TGCCAAACAC CAATGACCTC GTGATGGGGA AAAAGTGTGG AAATGGGTTC 
CTGGAGGAAG GAGAGCAGTG TGACTGTGGG GAGCCAGAGG AATGCACTAA TCCCTGCTGC 
AATGCAAATA ACTGCACTTT AAAGGCCGGC GCCAAGTGCG CTCATGGCGA ATGTTGCCAG 
GATTGTAGGC TGAAATCTGC AGGGACTCAG TGCCGGGAAA TGGCTGGATC CTGTGACCTT 
CCAGAATTCT GCACGGGGGA TGCCCCTTTC TGTCCCTCCA ATGTGTATAT GCTGGATGGG 
TCCTCATGTG CTTTTGGGGA GGCATACTGT TATAATGGCA TGTGTCTCAC TCACCAGCAG 
CAGTGTATCC ATCTTTGGGG TTCAGGAGCT GTGGTTGCCC CTGACTTCTG CTTCCAAGAT 
GTGAACAAAG CAGGAGACCA GTATGGAAAC TGCGGCAAGA ATGGAAGAGG ACAATTTGTG 
AAGTGTACAT CCCGAGATGC TAAGTGTGGA AAAATCCAAT GCCAGACGAG CTCAGAAAAG 
CCCAGGGACC CCAGCATGGT GAAGGTGGAC AACACCATTA TCATTAATGG CTACAAGATG 
AAGTGTCAGG GGGTACATGC CTACTCCATG CAAGAAGAGG AAGGTGACCC GGGGCTCGTA 
ATGACTGGGA CCAAGTGTGG AGACGGCATG GTATGCAAGG ATCGTCGCTG TCAGAATGCC 
TCCTTCTTTG AGCTGGACCA GTGCGTGTCT AAGTGTAATG GACATGGGGT CTGTAACAGC 
AACAGAAACT GCCATTGTCA TTCAGGCTGG GCCCCTCCTT ACTGTGACAA ACCTGGGCCA 
GGTGGCAGTC AAGACAGTGG CCCTGCACCT AATGACATTC CTGTGGGTGT CACTATTTTC 
TTGGTTATCC TGTTCCTGGT TCTGCTGCTG GGTTTGGCTA TTGCGATGGT CTACTGGTAT 
CGAAAACCAG GTTCTCTCCT GAACAAGTGG CTGAAGAAGA GCAAAGCAAA ATGCAGCTTA 
TGCAAAGCCA CCCAACCAAA GGCAAACAGA GCGTATTCAA GCAAGATCTT TACTATGCGC 
GATATATCCT ATCCAGTTAA ATCCGACAGC AAACAAACTC ACTCACGGGA CATATTCCAA 
GGAAAAACAA CTGCTGCTCA GAACAGTTCT CATCCTGTTA ACGTTGTGCG TCCTCTTCGT 
CCGGCTCCCA GCCCAGTGAT CCAACATGGG AAGCCTCTTA GGCCTCCTCC TCCTCCAATG 
AAACCGTCGC CCATTCTGCC TGCAAAAGAA GAAACCGTTC ATGTAAAACT GCTACCCCCC 
AAGAAACCTC TACCCTCTTG CCCTAGGCCG CAGCAGCTGA ACCCACCTTC CAAGCCACTG 
CCGGTGACTC CTGCTCACAA AGAGCCACTG TTGGTTATGA CACCGGCCAC ACATAACAAG 
GCTCCCATCA CCAACTCTGC TACCCAGCTT AAAGGGCCAC GCAGGCCAAT ACAAGGGGGC 
AAGGTCCAAG CTGCTGCTGC TGCCTTTCTT CAGAGAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819411.2
CDS	12..2753
Translation	MGTEGWLHTWLGLGAVFVGLLLAPVAALGAHQGERVTALWLQNERAKRSVELLDKVSGTPDRGEILVTSEGRRLILKVERNHRLFAPGYTETHYTDGQMVTLSPNHTEHCYYHGQVQGYDDSSVALTTCSGISGLIVLRTNDSYYLSPLEVSGKETHSLVRTEHLPIKGGSCGHEGHSGSTTSYFKEFTAPPGHHRVRRNVWRSQKYMELFIVADYSMFMKQNRNLGSTKQRVLEIANYVDKFYMSMNIKVALIGLEVWTERDQCDVNDDANDSLRSFLQWKQKLRSRKKHDNAQLITGVTFKGTTIGMAPLEGMCTAENSGGVSMDHSENAIGAAATMAHEIGHNFGMSHDNDGCCVEATPEQGGCIMAASTGHPFPRKFSRCSQQQLMSYFRKGGGMCLFNMPNTNDLVMGKKCGNGFLEEGEQCDCGEPEECTNPCCNANNCTLKAGAKCAHGECCQDCRLKSAGTQCREMAGSCDLPEFCTGDAPFCPSNVYMLDGSSCAFGEAYCYNGMCLTHQQQCIHLWGSGAVVAPDFCFQDVNKAGDQYGNCGKNGRGQFVKCTSRDAKCGKIQCQTSSEKPRDPSMVKVDNTIIINGYKMKCQGVHAYSMQEEEGDPGLVMTGTKCGDGMVCKDRRCQNASFFELDQCVSKCNGHGVCNSNRNCHCHSGWAPPYCDKPGPGGSQDSGPAPNDIPVGVTIFLVILFLVLLLGLAIAMVYWYRKPGSLLNKWLKKSKAKCSLCKATQPKANRAYSSKIFTMRDISYPVKSDSKQTHSRDIFQGKTTAAQNSSHPVNVVRPLRPAPSPVIQHGKPLRPPPPPMKPSPILPAKEETVHVKLLPPKKPLPSCPRPQQLNPPSKPLPVTPAHKEPLLVMTPATHNKAPITNSATQLKGPRRPIQGGKVQAAAAAFLQRK

Target ORF information:

RefSeq Version	XM_012963957.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ADAM metallopeptidase domain 33 (adam33), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963957.3

ORF Insert Sequence:

ATGGGGACGG AGGGTTGGCT GCACACATGG CTGGGACTGG GGGCTGTGTT TGTGGGGCTG 
CTCCTGGCTC CGGTGGCGGC ACTGGGGGCC CACCAAGGAG AGCGGGTGAC TGCCCTCTGG 
CTACAGAATG AAAGAGCTAA GAGATCAGTA GAGCTGCTGG ATAAGGTCAG TGGGACTCCA 
GACCGGGGAG AAATACTGGT GACATCAGAA GGAAGGCGGT TGATCCTGAA AGTGGAGAGA 
AATCACCGCC TGTTTGCGCC CGGCTACACT GAGACACATT ACACTGATGG GCAGATGGTC 
ACATTGTCTC CCAATCACAC GGAGCATTGC TATTACCATG GACAAGTGCA AGGCTATGAT 
GACTCCTCTG TCGCTCTCAC CACCTGCTCA GGAATCAGTG GTTTGATTGT GCTGAGAACT 
AATGACAGCT ACTACCTGAG TCCCCTGGAG GTATCAGGTA AAGAAACTCA TTCACTGGTC 
CGCACTGAAC ACCTGCCGAT CAAAGGTGGG AGCTGTGGGC ATGAAGGACA TTCAGGAAGC 
ACCACCTCTT ACTTCAAGGA GTTTACAGCA CCTCCTGGCC ATCACAGGGT AAGGAGGAAC 
GTCTGGAGGT CTCAGAAATA CATGGAGCTA TTTATTGTTG CAGATTACAG CATGTTTATG 
AAACAGAATC GGAATCTAGG CAGCACGAAG CAACGTGTCC TTGAAATTGC CAATTATGTA 
GACAAGTTCT ACATGTCCAT GAACATCAAG GTGGCTCTGA TAGGGCTGGA AGTCTGGACA 
GAGAGGGATC AATGTGATGT GAATGATGAT GCCAACGACA GCCTGAGATC GTTCCTACAG 
TGGAAGCAAA AGTTGAGGTC CCGGAAGAAG CACGATAACG CGCAGCTAAT CACAGGGGTG 
ACGTTTAAAG GAACAACCAT TGGGATGGCA CCACTGGAAG GCATGTGCAC TGCTGAGAAC 
TCAGGGGGAG TCAGTATGGA TCACTCAGAA AATGCAATTG GAGCTGCTGC AACAATGGCC 
CATGAAATTG GACACAATTT TGGAATGAGC CACGACAATG ACGGCTGCTG TGTGGAGGCC 
ACTCCGGAAC AAGGAGGCTG TATCATGGCG GCTTCCACTG GGCATCCCTT CCCTCGCAAG 
TTCAGCCGGT GCAGTCAGCA GCAGCTTATG AGTTATTTCA GGAAGGGGGG TGGAATGTGC 
CTGTTCAACA TGCCAAACAC CAATGACCTC GTGATGGGGA AAAAGTGTGG AAATGGGTTC 
CTGGAGGAAG GAGAGCAGTG TGACTGTGGG GAGCCAGAGG AATGCACTAA TCCCTGCTGC 
AATGCAAATA ACTGCACTTT AAAGGCCGGC GCCAAGTGCG CTCATGGCGA ATGTTGCCAG 
GATTGTAGGC TGAAATCTGC AGGGACTCAG TGCCGGGAAA TGGCTGGATC CTGTGACCTT 
CCAGAATTCT GCACGGGGGA TGCCCCTTTC TGTCCCTCCA ATGTGTATAT GCTGGATGGG 
TCCTCATGTG CTTTTGGGGA GGCATACTGT TATAATGGCA TGTGTCTCAC TCACCAGCAG 
CAGTGTATCC ATCTTTGGGG TTCAGGAGCT GTGGTTGCCC CTGACTTCTG CTTCCAAGAT 
GTGAACAAAG CAGGAGACCA GTATGGAAAC TGCGGCAAGA ATGGAAGAGG ACAATTTGTG 
AAGTGTACAT CCCGAGATGC TAAGTGTGGA AAAATCCAAT GCCAGACGAG CTCAGAAAAG 
CCCAGGGACC CCAGCATGGT GAAGGTGGAC AACACCATTA TCATTAATGG CTACAAGATG 
AAGTGTCAGG GGGTACATGC CTACTCCATG CAAGAAGAGG AAGGTGACCC GGGGCTCGTA 
ATGACTGGGA CCAAGTGTGG AGACGGCATG GTATGCAAGG ATCGTCGCTG TCAGAATGCC 
TCCTTCTTTG AGCTGGACCA GTGCGTGTCT AAGTGTAATG GACATGGGGT CTGTAACAGC 
AACAGAAACT GCCATTGTCA TTCAGGCTGG GCCCCTCCTT ACTGTGACAA ACCTGGGCCA 
GGTGGCAGTC AAGACAGTGG CCCTGCACCT AATGACATTC CTGTGGGTGT CACTATTTTC 
TTGGTTATCC TGTTCCTGGT TCTGCTGCTG GGTTTGGCTA TTGCGATGGT CTACTGGTAT 
CGAAAACCAG GTTCTCTCCT GAACAAGTGG CTGAAGAAGA GCAAAGCAAA ATGCAGCTTA 
TGCAAAGCCA CCCAACCAAA GGCAAACAGA GCGTATTCAA GCAAGATCTT TACTATGCGC 
GATATATCCT ATCCAGTTAA ATCCGACAGC AAACAAACTC ACTCACGGGA CATATTCCAA 
GGAAAAACAA CTGCTGCTCA GAACAGTTCT CATCCTGTTA ACGTTGTGCG TCCTCTTCGT 
CCGGCTCCCA GCCCAGTGAT CCAACATGGG AAGCCTCTTA GGCCTCCTCC TCCTCCAATG 
AAACCGTCGC CCATTCTGCC TGCAAAAGAA GAAACCGTTC ATGTAAAACT GCTACCCCCC 
AAGAAACCTC TACCCTCTTG CCCTAGGCCG CAGCAGCTGA ACCCACCTTC CAAGCCACTG 
CCGGTGACTC CTGCTCACAA AGAGCCACTG TTGGTTATGA CACCGGCCAC ACATAACAAG 
GCTCCCATCA CCAACTCTGC TACCCAGCTT AAAGGGCCAC GCAGGCCAAT ACAAGGGGGC 
AAGGTCCAAG CTGCTGCTGC TGCCTTTCTT CAGAGAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Roles of ADAM13-regulated Wnt activity in early Xenopus eye development.
Developmental biology363(1)147-54(2012 Mar)
Wei S,Xu G,Bridges LC,Williams P,Nakayama T,Shah A,Grainger RM,White JM,DeSimone DW

ADAM13 induces cranial neural crest by cleaving class B Ephrins and regulating Wnt signaling.
Developmental cell19(2)345-52(2010 Aug)
Wei S,Xu G,Bridges LC,Williams P,White JM,DeSimone DW

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adam33 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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