prag1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	prag1
Entrez Gene ID	100486686
Full Name	PEAK1 related, kinase-activating pseudokinase 1
Synonyms	pragmin,sgk223
General protein information	Preferred Names PEAK1 related, kinase-activating pseudokinase 1 Names tyrosine-protein kinase SgK223 homolog of rat pragma of Rnd2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002936454.4	XP_002936500.1	tyrosine-protein kinase SgK223
XM_002936454.5	XP_002936500.1	inactive tyrosine-protein kinase PRAG1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC533821	XP_005226093.1
Canis lupus familiaris (dog)	LOC482885	XP_540000.3
Xenopus tropicalis (tropical clawed frog)	LOC100486686	XP_002936500.1
Mus musculus (house mouse)	D8Ertd82e	NP_766499.2
Rattus norvegicus (Norway rat)	Pragmin	NP_001100785.1
Homo sapiens (human)	SGK223	NP_001074295.1
	LOC706349	XP_001094718.1
Pan troglodytes (chimpanzee)	LOC472692	XP_001136236.1
Gallus gallus (chicken)	SGK223	XP_420588.4

Gene Ontology

The following prag1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prag1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa04461	XM_002936454.5 Latest version!	Xenopus tropicalis PEAK1 related, kinase-activating pseudokinase 1 (prag1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OXa04461	XM_002936454.4	Xenopus tropicalis homolog of rat pragma of Rnd2 (sgk223), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prag1 ( XM_002936454.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936500.1 Xenopus tropicalis PEAK1 related, kinase-activating pseudokinase 1 (prag1), mRNA.

Price & Availability↑

CloneID	OXa04461
Clone ID Related Accession (Same CDS sequence)	XM_002936454.4 , XM_002936454.5
Accession Version	XM_002936454.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive tyrosine-protein kinase PRAG1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936454.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACACAG CCATCACATT AAACAGCCAG GGTGTGAAAA TGTCTGTGTG CAGCGAATTT 
GTGGAACACA TTTGGAAACC TGGATCCTGC AAGAACTGCT TTCACCCACG AAGTGACCAC 
AGACAGTTAC AACCACTCTC TGATGGGAAA AACGGAAACC TACCAGCATT GCATAGCCTT 
AATGGGATTA AACTTAAACC TGACATCAGT CAGGATGAAG ATACTGTAAT AGCGTCTTTG 
TATACCAAAC CCACGATTGC TGTGAAACCA ACCATGCTTA CCTCAGATAA TGAGATGTGG 
ACAGAGATTA ACAGCACCGA GACCCTAACT CAGGTTAGTT GGAAGATGTC TCAGACAAGC 
AATTCTCTTC TGAAATCTGG GGATATTTCT AAGTCAATCC TTGATAATTT CAACAAGCCC 
TTCAATCAGA AGTTCAGCGC TTTCAATGAC AATACTCCCA GCTATGTTTT TAATGGACTA 
AAGAGCCCTG ACAGAAAGAT GGGGATAAAT GCTGTGAATA ACCTAACCTA TGTGAGCCAC 
TTTAGTGGTC AGTCTGATAA AGCAACCTTT ATTAAGGAGA AATTGCCCAT TACCTTTGAA 
CAAACTACTA ATGGAAACCT AGTTAATGGA GACATAAAGA AATTAAGCTG CATTCAGGAA 
GAATCTACGT TACTTTCTTT AAAAAGCAGT AGTGGTTGCT CCTCTGGAAC TCTATCCTCT 
ACAGACAGGA ACACTCCACC TGGAACTTTA CTTACAAGAG GCACTTCAGT ACCTCAGGGA 
ACCGGTAAAT CTGGAATGGG ACTGTGTAAT TTGGATAACC CAACACCTAT AAAGCCTGCT 
AGAAAATTTC AAGATAAAAA CTGCTTAAAA AGCTTAGAGC TGGGATCTAG TAAGAACCTG 
AATTGCAGCA GTCCTAAAGT TACACTTCAT TCTGAGTCAG ATGCCCTGAC AAACAATTTG 
TCATATACCT TTACAAAGGA CAACCCTCTT GGATCACCTC CATACAAAGA TTCCCAGATT 
TCTGAGTTTC ACTTATCTAA GGATTGCAGG AAGAATCTAG TAAAACACTC CCAGCGGCTC 
ACACACCCAG AGGAACATCT ACAGTTCCAA GCTGAGGGAA CTAACAGAGA GCCCATTTAT 
GCAGAGAGCA CAAAAAGGAA AGGAGTTTTA GGTAGGTTGG AGATCAAGCC ACAATCTCAA 
AGGAATTCAG AAAAAAATGT TATACATCAA GACAGTCCTG TTGGTGAAGA GAAGCAAGAG 
ATTACTACAA CACAAGTTGC TGCCAAAATT ACAGTGATGG CAGCTCATAC AGAAGAAGAT 
AATAGAACAA TATTTTTGAG CAGTCCAGAT TCAGCAGTGG GGGTTCAGTG GACATGTAGT 
AGTCCATTAT CCTCTTCAGA AAATGCAAAT ATGTCACCAT TTGTCTGGGG AGAGAGAATC 
CAAGGAAGGC AAGAAATAAA AGCTGTTGAG GTAAATAAAA GGTATCTTGG TCCTTTATCT 
CCTAAAAGCC AAGGAAATAG CATTACTGCT GTGTCCTCCA TGCTCGTAAA ACCAGAGGAG 
TGCGCTAGTT TGAAACTCTC ATCACCAACC CACACTGCTG GAATTTTACA TTTGTCTTCT 
CAAAAAAAGG AATCACTTGA GGTAAATGAC AGGTGTCTGC AATCTACAGA GCGAAGGCAC 
AGGTACTATC AGGCACAATG GAGCAGGCAG TGTAAAATTG ATGAGGAAGA GGAGGAGGAA 
GTGTCAGTCT CCCAACCTTC AAGGATAAAT GTGGTCAGCA ATGGACAACT AACAAAGTCA 
AGTTTTAATT ATGAGGAAAA ATCCAGTGTG GAAGAGCAAA GAATAAGGAA AGGAATGAGT 
AAATCTGCTT CTTGTCCTGT AGAACTGTCT AAAGCAAACC ATGAACAAGA TGATGCCAGT 
CAGCCCCCCC CTCCTCCACC TAAGAAACAG ACACGGCATC CTCAACAGAT GAAGAAGAGT 
AGCACTGAAC TGGAAGTGGC AAGCCTTGGC TCAGTGGAGA GTCTGGGAGA GTCATTTAAG 
GCCACAAATG TTTCAAATGT GAACGTCAGT TTCAGCACTG GCTCCGTAGA CAGCCTTGAT 
TCCAGGACAT GCAGCGAAGA AGGTCACTCT TATGAAGCAG TGAATTCACC TTCTGTTTCT 
CCTGGAGGTG AAAAGAAACA TTTCACTAAG ATCTTATCTC TGGATTCTGG GCAAGATTCT 
CAACATGGAG CCATTCAACC ACCTCCTTTG CCTATGAAAA AATCAATGAA TAGAGCATCT 
TCAGTACCAG ACAATTCCGC TTTGGGAAGG ACAAATGTTT CAAGAACTTT ATTGAACTTA 
AACAACCAAC GATACAATCT TAGCCAATCA GAAAACAATG TATGTGGAGA GATAGCAACA 
CAATCCAGTT TACTTTTAAG TCCTGGAGAA AAACATGTAA TGTTTTCCTC CTCTGAATCC 
CTAGAGAAGT GTTGCAGAGG TAGCGGGCAC AGAAATGAAG TAAGAAGCAG GAACTCAGTT 
CAGGGGAAAG GTCCCCATGC TTTTTCTTCC TCTCAGTTGA GTGTTTCTAG TCATAGTACC 
TCAGGATCCA GCCTTCAGCT CCACCACCTG CTGAGCAACA TTGATAGCAA AGAAGGAATG 
TATGGCAAAC TTAGCGGACT TTATGCTCAG TCCATGCGCC GCTTAGTGAA CAAATGTGAA 
AACCATTTTA TGAGGGGACA AAAACAAGAA CACCACTTCA ATGAAAACAA TTGGTCACTC 
TTTAAGTTAA CAAGTAACAA GCCATGCTGT ATTGCTGGGG ATGCCATTTA CTATTGTGTC 
ACATGCTCAA GAGATCCAAA TAACAGCTAT TCTGTAAAGA TGTGCAAGGC AGAGGATACA 
AAATCTCAAT CAAACCTGTC ACTCCCAGTC CATTTTAATA TACAGCAAGA CTGTGGAAAT 
TTTGTGGCTA CTGTTCCATC AAGTATGTTA CATCCACCCA ACATACCCAA GGGTCCTTCT 
TCTAATGAGA GTTCACAGAC TGTGGGCCCA GCTAGTGAGC AGGAGTGTGT GGTGGTGATC 
ACTAGAGAGG TGCCCTATCT CACTGCTGCA GAGTTTGTAG AGGAGTCTGG GTCTACCCAC 
CAACTGCAGC CTGAGGTTTA TGAGCGCCAG GTTTGTCTTC TCTTACTCCA GCTCTGTAAT 
GGTTTGGAGC ATCTAAAGGA ACATGGCATT ATCCATAGAG ATCTGTGCTT GGAAAATCTC 
TTGCTTGTCC ATTGGCAGAA TTCTCCGGAA AAGATCAAAG ATGTAAAATA TGTTCCTAGG 
CTCATTGTTA GTAATTTCTC CAAAGCCAAA CAAAGATCTG GGTGTGAGGA GTCAAAGCCA 
AGGAGAGATA AAACTAGGCT GGCACCCGAA ATTATGGCAG CTTCCCAATA TAAAAAATTT 
GATGAGTTTC AAACAGGAAT CCTTATTTAT GAACTACTCC ATCAACCCAA TCCATTTGAG 
GTCCGCGCAA GCCTTCATGA ACATGATTAC AGCCAGAAAG ATTTGCCATC ACTTCCAGCT 
TTGTCTGTTT ATTCTCGTGG GCTTCAGCAC CTTGCCCACT TGCTGCTCGA GGCTGACCCA 
ATTAAACGTA TCCGTATTTC TGAAGCTAAG CGAGTGCTTC AATGTCTCTT ATGGGGCCCA 
AGAAAAGACT TGACTGATCA ACCCTTCAGC CATGAGGAAG CTTTCCACTG TGCTCTTCAG 
AACTGGATTG ACATGAAACG TGCCCTTCTG ATGATGAAGT TCGCTGAGCA GGCACTTGAG 
CCCCAGCAAA ATATTTCACT GGAGGACTGG CTCTGTTGCC AGTACTTAGC ATCAGCAGAT 
CCATGTTATC TGTATAAAAC ACTTAAACTC ATTAAGTTAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936500.1
CDS	295..4179
Translation	MHTAITLNSQGVKMSVCSEFVEHIWKPGSCKNCFHPRSDHRQLQPLSDGKNGNLPALHSLNGIKLKPDISQDEDTVIASLYTKPTIAVKPTMLTSDNEMWTEINSTETLTQVSWKMSQTSNSLLKSGDISKSILDNFNKPFNQKFSAFNDNTPSYVFNGLKSPDRKMGINAVNNLTYVSHFSGQSDKATFIKEKLPITFEQTTNGNLVNGDIKKLSCIQEESTLLSLKSSSGCSSGTLSSTDRNTPPGTLLTRGTSVPQGTGKSGMGLCNLDNPTPIKPARKFQDKNCLKSLELGSSKNLNCSSPKVTLHSESDALTNNLSYTFTKDNPLGSPPYKDSQISEFHLSKDCRKNLVKHSQRLTHPEEHLQFQAEGTNREPIYAESTKRKGVLGRLEIKPQSQRNSEKNVIHQDSPVGEEKQEITTTQVAAKITVMAAHTEEDNRTIFLSSPDSAVGVQWTCSSPLSSSENANMSPFVWGERIQGRQEIKAVEVNKRYLGPLSPKSQGNSITAVSSMLVKPEECASLKLSSPTHTAGILHLSSQKKESLEVNDRCLQSTERRHRYYQAQWSRQCKIDEEEEEEVSVSQPSRINVVSNGQLTKSSFNYEEKSSVEEQRIRKGMSKSASCPVELSKANHEQDDASQPPPPPPKKQTRHPQQMKKSSTELEVASLGSVESLGESFKATNVSNVNVSFSTGSVDSLDSRTCSEEGHSYEAVNSPSVSPGGEKKHFTKILSLDSGQDSQHGAIQPPPLPMKKSMNRASSVPDNSALGRTNVSRTLLNLNNQRYNLSQSENNVCGEIATQSSLLLSPGEKHVMFSSSESLEKCCRGSGHRNEVRSRNSVQGKGPHAFSSSQLSVSSHSTSGSSLQLHHLLSNIDSKEGMYGKLSGLYAQSMRRLVNKCENHFMRGQKQEHHFNENNWSLFKLTSNKPCCIAGDAIYYCVTCSRDPNNSYSVKMCKAEDTKSQSNLSLPVHFNIQQDCGNFVATVPSSMLHPPNIPKGPSSNESSQTVGPASEQECVVVITREVPYLTAAEFVEESGSTHQLQPEVYERQVCLLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHWQNSPEKIKDVKYVPRLIVSNFSKAKQRSGCEESKPRRDKTRLAPEIMAASQYKKFDEFQTGILIYELLHQPNPFEVRASLHEHDYSQKDLPSLPALSVYSRGLQHLAHLLLEADPIKRIRISEAKRVLQCLLWGPRKDLTDQPFSHEEAFHCALQNWIDMKRALLMMKFAEQALEPQQNISLEDWLCCQYLASADPCYLYKTLKLIKLI

Target ORF information:

RefSeq Version	XM_002936454.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis PEAK1 related, kinase-activating pseudokinase 1 (prag1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936454.5

ORF Insert Sequence:

ATGCACACAG CCATCACATT AAACAGCCAG GGTGTGAAAA TGTCTGTGTG CAGCGAATTT 
GTGGAACACA TTTGGAAACC TGGATCCTGC AAGAACTGCT TTCACCCACG AAGTGACCAC 
AGACAGTTAC AACCACTCTC TGATGGGAAA AACGGAAACC TACCAGCATT GCATAGCCTT 
AATGGGATTA AACTTAAACC TGACATCAGT CAGGATGAAG ATACTGTAAT AGCGTCTTTG 
TATACCAAAC CCACGATTGC TGTGAAACCA ACCATGCTTA CCTCAGATAA TGAGATGTGG 
ACAGAGATTA ACAGCACCGA GACCCTAACT CAGGTTAGTT GGAAGATGTC TCAGACAAGC 
AATTCTCTTC TGAAATCTGG GGATATTTCT AAGTCAATCC TTGATAATTT CAACAAGCCC 
TTCAATCAGA AGTTCAGCGC TTTCAATGAC AATACTCCCA GCTATGTTTT TAATGGACTA 
AAGAGCCCTG ACAGAAAGAT GGGGATAAAT GCTGTGAATA ACCTAACCTA TGTGAGCCAC 
TTTAGTGGTC AGTCTGATAA AGCAACCTTT ATTAAGGAGA AATTGCCCAT TACCTTTGAA 
CAAACTACTA ATGGAAACCT AGTTAATGGA GACATAAAGA AATTAAGCTG CATTCAGGAA 
GAATCTACGT TACTTTCTTT AAAAAGCAGT AGTGGTTGCT CCTCTGGAAC TCTATCCTCT 
ACAGACAGGA ACACTCCACC TGGAACTTTA CTTACAAGAG GCACTTCAGT ACCTCAGGGA 
ACCGGTAAAT CTGGAATGGG ACTGTGTAAT TTGGATAACC CAACACCTAT AAAGCCTGCT 
AGAAAATTTC AAGATAAAAA CTGCTTAAAA AGCTTAGAGC TGGGATCTAG TAAGAACCTG 
AATTGCAGCA GTCCTAAAGT TACACTTCAT TCTGAGTCAG ATGCCCTGAC AAACAATTTG 
TCATATACCT TTACAAAGGA CAACCCTCTT GGATCACCTC CATACAAAGA TTCCCAGATT 
TCTGAGTTTC ACTTATCTAA GGATTGCAGG AAGAATCTAG TAAAACACTC CCAGCGGCTC 
ACACACCCAG AGGAACATCT ACAGTTCCAA GCTGAGGGAA CTAACAGAGA GCCCATTTAT 
GCAGAGAGCA CAAAAAGGAA AGGAGTTTTA GGTAGGTTGG AGATCAAGCC ACAATCTCAA 
AGGAATTCAG AAAAAAATGT TATACATCAA GACAGTCCTG TTGGTGAAGA GAAGCAAGAG 
ATTACTACAA CACAAGTTGC TGCCAAAATT ACAGTGATGG CAGCTCATAC AGAAGAAGAT 
AATAGAACAA TATTTTTGAG CAGTCCAGAT TCAGCAGTGG GGGTTCAGTG GACATGTAGT 
AGTCCATTAT CCTCTTCAGA AAATGCAAAT ATGTCACCAT TTGTCTGGGG AGAGAGAATC 
CAAGGAAGGC AAGAAATAAA AGCTGTTGAG GTAAATAAAA GGTATCTTGG TCCTTTATCT 
CCTAAAAGCC AAGGAAATAG CATTACTGCT GTGTCCTCCA TGCTCGTAAA ACCAGAGGAG 
TGCGCTAGTT TGAAACTCTC ATCACCAACC CACACTGCTG GAATTTTACA TTTGTCTTCT 
CAAAAAAAGG AATCACTTGA GGTAAATGAC AGGTGTCTGC AATCTACAGA GCGAAGGCAC 
AGGTACTATC AGGCACAATG GAGCAGGCAG TGTAAAATTG ATGAGGAAGA GGAGGAGGAA 
GTGTCAGTCT CCCAACCTTC AAGGATAAAT GTGGTCAGCA ATGGACAACT AACAAAGTCA 
AGTTTTAATT ATGAGGAAAA ATCCAGTGTG GAAGAGCAAA GAATAAGGAA AGGAATGAGT 
AAATCTGCTT CTTGTCCTGT AGAACTGTCT AAAGCAAACC ATGAACAAGA TGATGCCAGT 
CAGCCCCCCC CTCCTCCACC TAAGAAACAG ACACGGCATC CTCAACAGAT GAAGAAGAGT 
AGCACTGAAC TGGAAGTGGC AAGCCTTGGC TCAGTGGAGA GTCTGGGAGA GTCATTTAAG 
GCCACAAATG TTTCAAATGT GAACGTCAGT TTCAGCACTG GCTCCGTAGA CAGCCTTGAT 
TCCAGGACAT GCAGCGAAGA AGGTCACTCT TATGAAGCAG TGAATTCACC TTCTGTTTCT 
CCTGGAGGTG AAAAGAAACA TTTCACTAAG ATCTTATCTC TGGATTCTGG GCAAGATTCT 
CAACATGGAG CCATTCAACC ACCTCCTTTG CCTATGAAAA AATCAATGAA TAGAGCATCT 
TCAGTACCAG ACAATTCCGC TTTGGGAAGG ACAAATGTTT CAAGAACTTT ATTGAACTTA 
AACAACCAAC GATACAATCT TAGCCAATCA GAAAACAATG TATGTGGAGA GATAGCAACA 
CAATCCAGTT TACTTTTAAG TCCTGGAGAA AAACATGTAA TGTTTTCCTC CTCTGAATCC 
CTAGAGAAGT GTTGCAGAGG TAGCGGGCAC AGAAATGAAG TAAGAAGCAG GAACTCAGTT 
CAGGGGAAAG GTCCCCATGC TTTTTCTTCC TCTCAGTTGA GTGTTTCTAG TCATAGTACC 
TCAGGATCCA GCCTTCAGCT CCACCACCTG CTGAGCAACA TTGATAGCAA AGAAGGAATG 
TATGGCAAAC TTAGCGGACT TTATGCTCAG TCCATGCGCC GCTTAGTGAA CAAATGTGAA 
AACCATTTTA TGAGGGGACA AAAACAAGAA CACCACTTCA ATGAAAACAA TTGGTCACTC 
TTTAAGTTAA CAAGTAACAA GCCATGCTGT ATTGCTGGGG ATGCCATTTA CTATTGTGTC 
ACATGCTCAA GAGATCCAAA TAACAGCTAT TCTGTAAAGA TGTGCAAGGC AGAGGATACA 
AAATCTCAAT CAAACCTGTC ACTCCCAGTC CATTTTAATA TACAGCAAGA CTGTGGAAAT 
TTTGTGGCTA CTGTTCCATC AAGTATGTTA CATCCACCCA ACATACCCAA GGGTCCTTCT 
TCTAATGAGA GTTCACAGAC TGTGGGCCCA GCTAGTGAGC AGGAGTGTGT GGTGGTGATC 
ACTAGAGAGG TGCCCTATCT CACTGCTGCA GAGTTTGTAG AGGAGTCTGG GTCTACCCAC 
CAACTGCAGC CTGAGGTTTA TGAGCGCCAG GTTTGTCTTC TCTTACTCCA GCTCTGTAAT 
GGTTTGGAGC ATCTAAAGGA ACATGGCATT ATCCATAGAG ATCTGTGCTT GGAAAATCTC 
TTGCTTGTCC ATTGGCAGAA TTCTCCGGAA AAGATCAAAG ATGTAAAATA TGTTCCTAGG 
CTCATTGTTA GTAATTTCTC CAAAGCCAAA CAAAGATCTG GGTGTGAGGA GTCAAAGCCA 
AGGAGAGATA AAACTAGGCT GGCACCCGAA ATTATGGCAG CTTCCCAATA TAAAAAATTT 
GATGAGTTTC AAACAGGAAT CCTTATTTAT GAACTACTCC ATCAACCCAA TCCATTTGAG 
GTCCGCGCAA GCCTTCATGA ACATGATTAC AGCCAGAAAG ATTTGCCATC ACTTCCAGCT 
TTGTCTGTTT ATTCTCGTGG GCTTCAGCAC CTTGCCCACT TGCTGCTCGA GGCTGACCCA 
ATTAAACGTA TCCGTATTTC TGAAGCTAAG CGAGTGCTTC AATGTCTCTT ATGGGGCCCA 
AGAAAAGACT TGACTGATCA ACCCTTCAGC CATGAGGAAG CTTTCCACTG TGCTCTTCAG 
AACTGGATTG ACATGAAACG TGCCCTTCTG ATGATGAAGT TCGCTGAGCA GGCACTTGAG 
CCCCAGCAAA ATATTTCACT GGAGGACTGG CTCTGTTGCC AGTACTTAGC ATCAGCAGAT 
CCATGTTATC TGTATAAAAC ACTTAAACTC ATTAAGTTAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prag1 ( XM_002936454.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936500.1 Xenopus tropicalis homolog of rat pragma of Rnd2 (sgk223), mRNA.

Price & Availability↑

CloneID	OXa04461
Clone ID Related Accession (Same CDS sequence)	XM_002936454.4 , XM_002936454.5
Accession Version	XM_002936454.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tyrosine-protein kinase SgK223
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002936454.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACACAG CCATCACATT AAACAGCCAG GGTGTGAAAA TGTCTGTGTG CAGCGAATTT 
GTGGAACACA TTTGGAAACC TGGATCCTGC AAGAACTGCT TTCACCCACG AAGTGACCAC 
AGACAGTTAC AACCACTCTC TGATGGGAAA AACGGAAACC TACCAGCATT GCATAGCCTT 
AATGGGATTA AACTTAAACC TGACATCAGT CAGGATGAAG ATACTGTAAT AGCGTCTTTG 
TATACCAAAC CCACGATTGC TGTGAAACCA ACCATGCTTA CCTCAGATAA TGAGATGTGG 
ACAGAGATTA ACAGCACCGA GACCCTAACT CAGGTTAGTT GGAAGATGTC TCAGACAAGC 
AATTCTCTTC TGAAATCTGG GGATATTTCT AAGTCAATCC TTGATAATTT CAACAAGCCC 
TTCAATCAGA AGTTCAGCGC TTTCAATGAC AATACTCCCA GCTATGTTTT TAATGGACTA 
AAGAGCCCTG ACAGAAAGAT GGGGATAAAT GCTGTGAATA ACCTAACCTA TGTGAGCCAC 
TTTAGTGGTC AGTCTGATAA AGCAACCTTT ATTAAGGAGA AATTGCCCAT TACCTTTGAA 
CAAACTACTA ATGGAAACCT AGTTAATGGA GACATAAAGA AATTAAGCTG CATTCAGGAA 
GAATCTACGT TACTTTCTTT AAAAAGCAGT AGTGGTTGCT CCTCTGGAAC TCTATCCTCT 
ACAGACAGGA ACACTCCACC TGGAACTTTA CTTACAAGAG GCACTTCAGT ACCTCAGGGA 
ACCGGTAAAT CTGGAATGGG ACTGTGTAAT TTGGATAACC CAACACCTAT AAAGCCTGCT 
AGAAAATTTC AAGATAAAAA CTGCTTAAAA AGCTTAGAGC TGGGATCTAG TAAGAACCTG 
AATTGCAGCA GTCCTAAAGT TACACTTCAT TCTGAGTCAG ATGCCCTGAC AAACAATTTG 
TCATATACCT TTACAAAGGA CAACCCTCTT GGATCACCTC CATACAAAGA TTCCCAGATT 
TCTGAGTTTC ACTTATCTAA GGATTGCAGG AAGAATCTAG TAAAACACTC CCAGCGGCTC 
ACACACCCAG AGGAACATCT ACAGTTCCAA GCTGAGGGAA CTAACAGAGA GCCCATTTAT 
GCAGAGAGCA CAAAAAGGAA AGGAGTTTTA GGTAGGTTGG AGATCAAGCC ACAATCTCAA 
AGGAATTCAG AAAAAAATGT TATACATCAA GACAGTCCTG TTGGTGAAGA GAAGCAAGAG 
ATTACTACAA CACAAGTTGC TGCCAAAATT ACAGTGATGG CAGCTCATAC AGAAGAAGAT 
AATAGAACAA TATTTTTGAG CAGTCCAGAT TCAGCAGTGG GGGTTCAGTG GACATGTAGT 
AGTCCATTAT CCTCTTCAGA AAATGCAAAT ATGTCACCAT TTGTCTGGGG AGAGAGAATC 
CAAGGAAGGC AAGAAATAAA AGCTGTTGAG GTAAATAAAA GGTATCTTGG TCCTTTATCT 
CCTAAAAGCC AAGGAAATAG CATTACTGCT GTGTCCTCCA TGCTCGTAAA ACCAGAGGAG 
TGCGCTAGTT TGAAACTCTC ATCACCAACC CACACTGCTG GAATTTTACA TTTGTCTTCT 
CAAAAAAAGG AATCACTTGA GGTAAATGAC AGGTGTCTGC AATCTACAGA GCGAAGGCAC 
AGGTACTATC AGGCACAATG GAGCAGGCAG TGTAAAATTG ATGAGGAAGA GGAGGAGGAA 
GTGTCAGTCT CCCAACCTTC AAGGATAAAT GTGGTCAGCA ATGGACAACT AACAAAGTCA 
AGTTTTAATT ATGAGGAAAA ATCCAGTGTG GAAGAGCAAA GAATAAGGAA AGGAATGAGT 
AAATCTGCTT CTTGTCCTGT AGAACTGTCT AAAGCAAACC ATGAACAAGA TGATGCCAGT 
CAGCCCCCCC CTCCTCCACC TAAGAAACAG ACACGGCATC CTCAACAGAT GAAGAAGAGT 
AGCACTGAAC TGGAAGTGGC AAGCCTTGGC TCAGTGGAGA GTCTGGGAGA GTCATTTAAG 
GCCACAAATG TTTCAAATGT GAACGTCAGT TTCAGCACTG GCTCCGTAGA CAGCCTTGAT 
TCCAGGACAT GCAGCGAAGA AGGTCACTCT TATGAAGCAG TGAATTCACC TTCTGTTTCT 
CCTGGAGGTG AAAAGAAACA TTTCACTAAG ATCTTATCTC TGGATTCTGG GCAAGATTCT 
CAACATGGAG CCATTCAACC ACCTCCTTTG CCTATGAAAA AATCAATGAA TAGAGCATCT 
TCAGTACCAG ACAATTCCGC TTTGGGAAGG ACAAATGTTT CAAGAACTTT ATTGAACTTA 
AACAACCAAC GATACAATCT TAGCCAATCA GAAAACAATG TATGTGGAGA GATAGCAACA 
CAATCCAGTT TACTTTTAAG TCCTGGAGAA AAACATGTAA TGTTTTCCTC CTCTGAATCC 
CTAGAGAAGT GTTGCAGAGG TAGCGGGCAC AGAAATGAAG TAAGAAGCAG GAACTCAGTT 
CAGGGGAAAG GTCCCCATGC TTTTTCTTCC TCTCAGTTGA GTGTTTCTAG TCATAGTACC 
TCAGGATCCA GCCTTCAGCT CCACCACCTG CTGAGCAACA TTGATAGCAA AGAAGGAATG 
TATGGCAAAC TTAGCGGACT TTATGCTCAG TCCATGCGCC GCTTAGTGAA CAAATGTGAA 
AACCATTTTA TGAGGGGACA AAAACAAGAA CACCACTTCA ATGAAAACAA TTGGTCACTC 
TTTAAGTTAA CAAGTAACAA GCCATGCTGT ATTGCTGGGG ATGCCATTTA CTATTGTGTC 
ACATGCTCAA GAGATCCAAA TAACAGCTAT TCTGTAAAGA TGTGCAAGGC AGAGGATACA 
AAATCTCAAT CAAACCTGTC ACTCCCAGTC CATTTTAATA TACAGCAAGA CTGTGGAAAT 
TTTGTGGCTA CTGTTCCATC AAGTATGTTA CATCCACCCA ACATACCCAA GGGTCCTTCT 
TCTAATGAGA GTTCACAGAC TGTGGGCCCA GCTAGTGAGC AGGAGTGTGT GGTGGTGATC 
ACTAGAGAGG TGCCCTATCT CACTGCTGCA GAGTTTGTAG AGGAGTCTGG GTCTACCCAC 
CAACTGCAGC CTGAGGTTTA TGAGCGCCAG GTTTGTCTTC TCTTACTCCA GCTCTGTAAT 
GGTTTGGAGC ATCTAAAGGA ACATGGCATT ATCCATAGAG ATCTGTGCTT GGAAAATCTC 
TTGCTTGTCC ATTGGCAGAA TTCTCCGGAA AAGATCAAAG ATGTAAAATA TGTTCCTAGG 
CTCATTGTTA GTAATTTCTC CAAAGCCAAA CAAAGATCTG GGTGTGAGGA GTCAAAGCCA 
AGGAGAGATA AAACTAGGCT GGCACCCGAA ATTATGGCAG CTTCCCAATA TAAAAAATTT 
GATGAGTTTC AAACAGGAAT CCTTATTTAT GAACTACTCC ATCAACCCAA TCCATTTGAG 
GTCCGCGCAA GCCTTCATGA ACATGATTAC AGCCAGAAAG ATTTGCCATC ACTTCCAGCT 
TTGTCTGTTT ATTCTCGTGG GCTTCAGCAC CTTGCCCACT TGCTGCTCGA GGCTGACCCA 
ATTAAACGTA TCCGTATTTC TGAAGCTAAG CGAGTGCTTC AATGTCTCTT ATGGGGCCCA 
AGAAAAGACT TGACTGATCA ACCCTTCAGC CATGAGGAAG CTTTCCACTG TGCTCTTCAG 
AACTGGATTG ACATGAAACG TGCCCTTCTG ATGATGAAGT TCGCTGAGCA GGCACTTGAG 
CCCCAGCAAA ATATTTCACT GGAGGACTGG CTCTGTTGCC AGTACTTAGC ATCAGCAGAT 
CCATGTTATC TGTATAAAAC ACTTAAACTC ATTAAGTTAA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936500.1
CDS	297..4181
Translation	MHTAITLNSQGVKMSVCSEFVEHIWKPGSCKNCFHPRSDHRQLQPLSDGKNGNLPALHSLNGIKLKPDISQDEDTVIASLYTKPTIAVKPTMLTSDNEMWTEINSTETLTQVSWKMSQTSNSLLKSGDISKSILDNFNKPFNQKFSAFNDNTPSYVFNGLKSPDRKMGINAVNNLTYVSHFSGQSDKATFIKEKLPITFEQTTNGNLVNGDIKKLSCIQEESTLLSLKSSSGCSSGTLSSTDRNTPPGTLLTRGTSVPQGTGKSGMGLCNLDNPTPIKPARKFQDKNCLKSLELGSSKNLNCSSPKVTLHSESDALTNNLSYTFTKDNPLGSPPYKDSQISEFHLSKDCRKNLVKHSQRLTHPEEHLQFQAEGTNREPIYAESTKRKGVLGRLEIKPQSQRNSEKNVIHQDSPVGEEKQEITTTQVAAKITVMAAHTEEDNRTIFLSSPDSAVGVQWTCSSPLSSSENANMSPFVWGERIQGRQEIKAVEVNKRYLGPLSPKSQGNSITAVSSMLVKPEECASLKLSSPTHTAGILHLSSQKKESLEVNDRCLQSTERRHRYYQAQWSRQCKIDEEEEEEVSVSQPSRINVVSNGQLTKSSFNYEEKSSVEEQRIRKGMSKSASCPVELSKANHEQDDASQPPPPPPKKQTRHPQQMKKSSTELEVASLGSVESLGESFKATNVSNVNVSFSTGSVDSLDSRTCSEEGHSYEAVNSPSVSPGGEKKHFTKILSLDSGQDSQHGAIQPPPLPMKKSMNRASSVPDNSALGRTNVSRTLLNLNNQRYNLSQSENNVCGEIATQSSLLLSPGEKHVMFSSSESLEKCCRGSGHRNEVRSRNSVQGKGPHAFSSSQLSVSSHSTSGSSLQLHHLLSNIDSKEGMYGKLSGLYAQSMRRLVNKCENHFMRGQKQEHHFNENNWSLFKLTSNKPCCIAGDAIYYCVTCSRDPNNSYSVKMCKAEDTKSQSNLSLPVHFNIQQDCGNFVATVPSSMLHPPNIPKGPSSNESSQTVGPASEQECVVVITREVPYLTAAEFVEESGSTHQLQPEVYERQVCLLLLQLCNGLEHLKEHGIIHRDLCLENLLLVHWQNSPEKIKDVKYVPRLIVSNFSKAKQRSGCEESKPRRDKTRLAPEIMAASQYKKFDEFQTGILIYELLHQPNPFEVRASLHEHDYSQKDLPSLPALSVYSRGLQHLAHLLLEADPIKRIRISEAKRVLQCLLWGPRKDLTDQPFSHEEAFHCALQNWIDMKRALLMMKFAEQALEPQQNISLEDWLCCQYLASADPCYLYKTLKLIKLI

Target ORF information:

RefSeq Version	XM_002936454.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis homolog of rat pragma of Rnd2 (sgk223), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936454.4

ORF Insert Sequence:

ATGCACACAG CCATCACATT AAACAGCCAG GGTGTGAAAA TGTCTGTGTG CAGCGAATTT 
GTGGAACACA TTTGGAAACC TGGATCCTGC AAGAACTGCT TTCACCCACG AAGTGACCAC 
AGACAGTTAC AACCACTCTC TGATGGGAAA AACGGAAACC TACCAGCATT GCATAGCCTT 
AATGGGATTA AACTTAAACC TGACATCAGT CAGGATGAAG ATACTGTAAT AGCGTCTTTG 
TATACCAAAC CCACGATTGC TGTGAAACCA ACCATGCTTA CCTCAGATAA TGAGATGTGG 
ACAGAGATTA ACAGCACCGA GACCCTAACT CAGGTTAGTT GGAAGATGTC TCAGACAAGC 
AATTCTCTTC TGAAATCTGG GGATATTTCT AAGTCAATCC TTGATAATTT CAACAAGCCC 
TTCAATCAGA AGTTCAGCGC TTTCAATGAC AATACTCCCA GCTATGTTTT TAATGGACTA 
AAGAGCCCTG ACAGAAAGAT GGGGATAAAT GCTGTGAATA ACCTAACCTA TGTGAGCCAC 
TTTAGTGGTC AGTCTGATAA AGCAACCTTT ATTAAGGAGA AATTGCCCAT TACCTTTGAA 
CAAACTACTA ATGGAAACCT AGTTAATGGA GACATAAAGA AATTAAGCTG CATTCAGGAA 
GAATCTACGT TACTTTCTTT AAAAAGCAGT AGTGGTTGCT CCTCTGGAAC TCTATCCTCT 
ACAGACAGGA ACACTCCACC TGGAACTTTA CTTACAAGAG GCACTTCAGT ACCTCAGGGA 
ACCGGTAAAT CTGGAATGGG ACTGTGTAAT TTGGATAACC CAACACCTAT AAAGCCTGCT 
AGAAAATTTC AAGATAAAAA CTGCTTAAAA AGCTTAGAGC TGGGATCTAG TAAGAACCTG 
AATTGCAGCA GTCCTAAAGT TACACTTCAT TCTGAGTCAG ATGCCCTGAC AAACAATTTG 
TCATATACCT TTACAAAGGA CAACCCTCTT GGATCACCTC CATACAAAGA TTCCCAGATT 
TCTGAGTTTC ACTTATCTAA GGATTGCAGG AAGAATCTAG TAAAACACTC CCAGCGGCTC 
ACACACCCAG AGGAACATCT ACAGTTCCAA GCTGAGGGAA CTAACAGAGA GCCCATTTAT 
GCAGAGAGCA CAAAAAGGAA AGGAGTTTTA GGTAGGTTGG AGATCAAGCC ACAATCTCAA 
AGGAATTCAG AAAAAAATGT TATACATCAA GACAGTCCTG TTGGTGAAGA GAAGCAAGAG 
ATTACTACAA CACAAGTTGC TGCCAAAATT ACAGTGATGG CAGCTCATAC AGAAGAAGAT 
AATAGAACAA TATTTTTGAG CAGTCCAGAT TCAGCAGTGG GGGTTCAGTG GACATGTAGT 
AGTCCATTAT CCTCTTCAGA AAATGCAAAT ATGTCACCAT TTGTCTGGGG AGAGAGAATC 
CAAGGAAGGC AAGAAATAAA AGCTGTTGAG GTAAATAAAA GGTATCTTGG TCCTTTATCT 
CCTAAAAGCC AAGGAAATAG CATTACTGCT GTGTCCTCCA TGCTCGTAAA ACCAGAGGAG 
TGCGCTAGTT TGAAACTCTC ATCACCAACC CACACTGCTG GAATTTTACA TTTGTCTTCT 
CAAAAAAAGG AATCACTTGA GGTAAATGAC AGGTGTCTGC AATCTACAGA GCGAAGGCAC 
AGGTACTATC AGGCACAATG GAGCAGGCAG TGTAAAATTG ATGAGGAAGA GGAGGAGGAA 
GTGTCAGTCT CCCAACCTTC AAGGATAAAT GTGGTCAGCA ATGGACAACT AACAAAGTCA 
AGTTTTAATT ATGAGGAAAA ATCCAGTGTG GAAGAGCAAA GAATAAGGAA AGGAATGAGT 
AAATCTGCTT CTTGTCCTGT AGAACTGTCT AAAGCAAACC ATGAACAAGA TGATGCCAGT 
CAGCCCCCCC CTCCTCCACC TAAGAAACAG ACACGGCATC CTCAACAGAT GAAGAAGAGT 
AGCACTGAAC TGGAAGTGGC AAGCCTTGGC TCAGTGGAGA GTCTGGGAGA GTCATTTAAG 
GCCACAAATG TTTCAAATGT GAACGTCAGT TTCAGCACTG GCTCCGTAGA CAGCCTTGAT 
TCCAGGACAT GCAGCGAAGA AGGTCACTCT TATGAAGCAG TGAATTCACC TTCTGTTTCT 
CCTGGAGGTG AAAAGAAACA TTTCACTAAG ATCTTATCTC TGGATTCTGG GCAAGATTCT 
CAACATGGAG CCATTCAACC ACCTCCTTTG CCTATGAAAA AATCAATGAA TAGAGCATCT 
TCAGTACCAG ACAATTCCGC TTTGGGAAGG ACAAATGTTT CAAGAACTTT ATTGAACTTA 
AACAACCAAC GATACAATCT TAGCCAATCA GAAAACAATG TATGTGGAGA GATAGCAACA 
CAATCCAGTT TACTTTTAAG TCCTGGAGAA AAACATGTAA TGTTTTCCTC CTCTGAATCC 
CTAGAGAAGT GTTGCAGAGG TAGCGGGCAC AGAAATGAAG TAAGAAGCAG GAACTCAGTT 
CAGGGGAAAG GTCCCCATGC TTTTTCTTCC TCTCAGTTGA GTGTTTCTAG TCATAGTACC 
TCAGGATCCA GCCTTCAGCT CCACCACCTG CTGAGCAACA TTGATAGCAA AGAAGGAATG 
TATGGCAAAC TTAGCGGACT TTATGCTCAG TCCATGCGCC GCTTAGTGAA CAAATGTGAA 
AACCATTTTA TGAGGGGACA AAAACAAGAA CACCACTTCA ATGAAAACAA TTGGTCACTC 
TTTAAGTTAA CAAGTAACAA GCCATGCTGT ATTGCTGGGG ATGCCATTTA CTATTGTGTC 
ACATGCTCAA GAGATCCAAA TAACAGCTAT TCTGTAAAGA TGTGCAAGGC AGAGGATACA 
AAATCTCAAT CAAACCTGTC ACTCCCAGTC CATTTTAATA TACAGCAAGA CTGTGGAAAT 
TTTGTGGCTA CTGTTCCATC AAGTATGTTA CATCCACCCA ACATACCCAA GGGTCCTTCT 
TCTAATGAGA GTTCACAGAC TGTGGGCCCA GCTAGTGAGC AGGAGTGTGT GGTGGTGATC 
ACTAGAGAGG TGCCCTATCT CACTGCTGCA GAGTTTGTAG AGGAGTCTGG GTCTACCCAC 
CAACTGCAGC CTGAGGTTTA TGAGCGCCAG GTTTGTCTTC TCTTACTCCA GCTCTGTAAT 
GGTTTGGAGC ATCTAAAGGA ACATGGCATT ATCCATAGAG ATCTGTGCTT GGAAAATCTC 
TTGCTTGTCC ATTGGCAGAA TTCTCCGGAA AAGATCAAAG ATGTAAAATA TGTTCCTAGG 
CTCATTGTTA GTAATTTCTC CAAAGCCAAA CAAAGATCTG GGTGTGAGGA GTCAAAGCCA 
AGGAGAGATA AAACTAGGCT GGCACCCGAA ATTATGGCAG CTTCCCAATA TAAAAAATTT 
GATGAGTTTC AAACAGGAAT CCTTATTTAT GAACTACTCC ATCAACCCAA TCCATTTGAG 
GTCCGCGCAA GCCTTCATGA ACATGATTAC AGCCAGAAAG ATTTGCCATC ACTTCCAGCT 
TTGTCTGTTT ATTCTCGTGG GCTTCAGCAC CTTGCCCACT TGCTGCTCGA GGCTGACCCA 
ATTAAACGTA TCCGTATTTC TGAAGCTAAG CGAGTGCTTC AATGTCTCTT ATGGGGCCCA 
AGAAAAGACT TGACTGATCA ACCCTTCAGC CATGAGGAAG CTTTCCACTG TGCTCTTCAG 
AACTGGATTG ACATGAAACG TGCCCTTCTG ATGATGAAGT TCGCTGAGCA GGCACTTGAG 
CCCCAGCAAA ATATTTCACT GGAGGACTGG CTCTGTTGCC AGTACTTAGC ATCAGCAGAT 
CCATGTTATC TGTATAAAAC ACTTAAACTC ATTAAGTTAA TTTAA