chtf18 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	chtf18
Entrez Gene ID	100380057
Full Name	chromosome transmission fidelity factor 18
Synonyms	ctf18
General protein information	Preferred Names chromosome transmission fidelity factor 18 Names chromosome transmission fidelity protein 18 homolog CTF18, chromosome transmission fidelity factor 18 homolog
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002935859.4	XP_002935905.2	chromosome transmission fidelity protein 18 homolog
XM_002935859.5	XP_002935905.3	chromosome transmission fidelity protein 18 homolog

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Chtf18	NP_001099243.1
Pan troglodytes (chimpanzee)	CHTF18	XP_003952800.1
	CHTF18	XP_001087813.1
Bos taurus (cattle)	CHTF18	NP_001179389.1
Mus musculus (house mouse)	Chtf18	NP_663384.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008869	XP_319609.4
Oryza sativa (rice)	Os03g0264800	NP_001049644.2
Canis lupus familiaris (dog)	CHTF18	XP_547205.4
Gallus gallus (chicken)	CHTF18	NP_001264989.1
Danio rerio (zebrafish)	chtf18	NP_001103572.2
Arabidopsis thaliana (thale cress)	CTF18	NP_171966.2
Homo sapiens (human)	CHTF18	NP_071375.1
Drosophila melanogaster (fruit fly)	cutlet	NP_787969.1
Xenopus tropicalis (tropical clawed frog)	chtf18	XP_002935905.2

Gene Ontology

The following chtf18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chtf18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa66261	XM_002935859.5 Latest version!	Xenopus tropicalis chromosome transmission fidelity factor 18 (chtf18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa16479	XM_002935859.4 Latest version!	Xenopus tropicalis chromosome transmission fidelity factor 18 (chtf18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chtf18 ( XM_002935859.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935905.3 Xenopus tropicalis chromosome transmission fidelity factor 18 (chtf18), mRNA.

Price & Availability↑

CloneID	OXa66261
Clone ID Related Accession (Same CDS sequence)	XM_002935859.5
Accession Version	XM_002935859.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chromosome transmission fidelity protein 18 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935859.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAAT ATGACTTGTA CGGAGTGGAG GATGATTTCG AGAGTCAGTT CGCCTCGGAG 
CTGGAAGTGC TGGCCGAGTT TGAGGATGAC GTACCTTCCC GTCCCGCTCC CCAAAGGTCC 
CAGCTACGCA GCAAACTTTC CTTCGAGGAG GCAATCTCTG CCGGGGATCG TGTAAAGAGC 
AACGCCGACA GCAAAACAAC TAGTGAGAGA AACGGCGATG CTCTCGCTTG CGTCAATACC 
AGCAAGACTA AAAAGCGAGA CGCAAGCTGC CTTGACTTTG AAGAGGATGA ATTCTCAGAC 
AGCTTCTTTG AGCCTCCTGT CACTCCCAAA CACAAACGGC CAAGAATTGA AGCTGTTAAG 
AAGCTAGACT TTGGAAGTGA TGAATATGCA GGCAACAGTA CTGCCCTCAG TGATGATATC 
ACACCTCCAC CCTCTCCTCA TTATACTCAG AAGAAAAGTG AAAGGGATTC CAAGTTCCCA 
AGCTGCGATG CCTTTGAGGT GAGCGACATG GCACCCCTGC AGGTAACGCC CACAGAATCG 
GAGCAGAAGC GCGTGCTTAA GCGTCCCCCT GTGCTGGAGG ATTACATTAA CGTGACATCC 
ACTGACGGGT CTAGGGTGTA TATGACATTA AAAGAGGATA ATGATGGCTC AGGGACTCAG 
CAAAAGCAAG GGAACGTGAA GTGGAACAGT GGTCAGCAGC TGCATCTCCT GGGGGTTCCA 
TTCTCCTACT TAAAGGAACA GGTTAACGAT GAGCGCCGAC GGCAGGTGTT AGAAGAATCC 
CAGAAACTTA CAGAAATGCT GAACAGTCAA ATTAATGATG AATTTGGAGA GGACGATAGT 
GAAACACTGG AAAATGGAGA TAATGCTGGA GAAGAGGATG GCGAAAATGA ATCTTCATCC 
CACTCGCTGT GGGTAGACTG CTTCACTCCC AGGCACTACA CTGAATTGCT CAGTGATGAT 
TACACAAACC GCTGCCTACT GAAATGGTTA AAGCTGTGGG ACACTGTGGT GTTTGGGAAA 
GAGAGAGTGG TGAAGAAACC CAAGGCAAAT GTGGACCCAC GCGCCAACTT CAAGAATCAG 
AAAGAGCAGC AGAGCAAGTT TAAGAGCAAA GCTCAGATCA CCGAGGAGAT CCTGGAAGCT 
GAACTGGATC AGCACAACAG ACCCAAAAAC AAGGTTGCAC TTCTTTGCGG CCCACCGGGC 
CTTGGAAAGA CCACACTGGC ACATGTAATA GCAAGGCACG CAGGATACAA TGTGGTGGAA 
ATGAATGCCA GTGATGATCG GAGCCCCGAG ATCTTTAAAA CCCGCATAGA AGCAGCGACT 
CAGATGAAAT CTGTTTTGGG TGTTGATGAG AGACCCAACT GCCTCATTAT AGATGAAATT 
GATGGTGCTC CAGCAGTCTC CATCAACATG CTGCTGAGTT TAATCAACCG AAAAGACGCC 
AAGGAAACTG AGGGTGGCAC AGATGTGACA ACAGGGAAGA AGAAAAAGAA AGAAGGTGGT 
TTACTGCTCA GGCCAATCAT CTGTATCTGC AATGATCAAT ATGTCCCTTC CTTACGGCTG 
CTGAGACAGC AAGCTTTCAT GCTGAATTTT CCTCAGACTC TGCCTTCCAG ACTGGTGCAA 
AGGCTGTATG AGATCACCAT TAAGCAAGGT ATGAAAGCAG ACACTGGGGC CCTCATGGCT 
TTGTGTGAAA AGACAGAGAA TGATATCAGA TCCTGTATTA ATACATTGCA GTTCCTACAC 
GGCCGAGGAA AGAAGGAGCT CAATATGCGC AGTGTGCAGA CCATGAAGAT CGGCCTGAAG 
GATCAAAACA AAGGCCTGTT CTCTGTTTGG CAGGAGATCT TCCAGCTTCC CAAGATTCAG 
AGGAAACGTA TCGGCCAAGA AATTGCAATT AAAGACCTCC ATCTTTTTTT GGGGTCAGGA 
AATGAATCTC TCGGTGGGGT GGGGAAGGCA CCATTAAGCG CCGTGGCTCA GAGATTTCAT 
CACATTCTGC ATCTCTCCAC TTCCACGGGA GAGTACGAGA AGCTCACCAT GGGTTTGTAC 
GACAATTTCC TAAACATGAA GGTGAAAGAG TCCAACTTCA GTACAGTGTG CCTGGCTTTG 
GATTGGCTGG AGTTCACGGA CATTGTGAAC ACGACTATAA TGCACGGGCA GAATTTCCAG 
CTCATGAGAT ACCTCCCCTT CCTGCCTGTA GCTTTCCATC TCCTGTTTGC AGCCTCAAAT 
GTGCCGCGCA TTGCATACCC CAACAGCCAC TATGAGGCAC AGTCCAAACT TAATCAAATG 
CAAAACCTAC TGAATGCAAT GGTGGCCGAA ATTTCTCCAG CTATCCGAAC GCGCGTGGGG 
CCTCAGTCCT TGGTGCTTGA TGCTCTGTGC CTTCTCCTGG ATGTGATCTC TCCTAAACTG 
CGCCCGGTAA ACACGCAGCT GTTCAGTACC AAAGAGAAGC AGCAGCTAGC CGAGCTTATT 
AATACTATGC TAGCCTATAA CCTGACCTAC CACCAGGAGC GGACCATGGA AGGGCAGTAT 
GTCTACAAGC TGGATCCGAA TGTTGAAGAT GTATGCAGGT TTCCAGATCT TCCAATCCGT 
AAACCATTGA CTTACCAAGC CAAACAGCTC ATAGCGCGCG AGATTGAACT GGAGAGAATG 
AGGAGGACAG AAGCATTCCA GCAAGCGAGA AATGCAGGAA GGGACAATGC CAGCGCCAAT 
GCCGCTAAAC CAACCACTGA AACCAAAGGT GCCAAAAGCA CGGCCAAACC CGCGGCTTTA 
AACCACGAAC AGCGGTTGGA GAATATCATG AAGAAGGCAG CAGCGTTTGA GGAGAAGCCT 
GAAAAAGATT TCTTTGGTCG CCAAATAGTC AAGAAGGTGG CAGCGCCAGT GGCAGCAAGT 
GCAAACCAGG AGGAATCGGT GGAGAGAAGA ATTGGGAAGG CGGTGGGAAA CAGCGATGTT 
TGGTTTCGCT TTAATGAGGG TGTTTCTAAT GCTGTGCGGC GGAATATATA CATTAAGGAC 
CTCCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935905.3
CDS	105..3113
Translation	MDEYDLYGVEDDFESQFASELEVLAEFEDDVPSRPAPQRSQLRSKLSFEEAISAGDRVKSNADSKTTSERNGDALACVNTSKTKKRDASCLDFEEDEFSDSFFEPPVTPKHKRPRIEAVKKLDFGSDEYAGNSTALSDDITPPPSPHYTQKKSERDSKFPSCDAFEVSDMAPLQVTPTESEQKRVLKRPPVLEDYINVTSTDGSRVYMTLKEDNDGSGTQQKQGNVKWNSGQQLHLLGVPFSYLKEQVNDERRRQVLEESQKLTEMLNSQINDEFGEDDSETLENGDNAGEEDGENESSSHSLWVDCFTPRHYTELLSDDYTNRCLLKWLKLWDTVVFGKERVVKKPKANVDPRANFKNQKEQQSKFKSKAQITEEILEAELDQHNRPKNKVALLCGPPGLGKTTLAHVIARHAGYNVVEMNASDDRSPEIFKTRIEAATQMKSVLGVDERPNCLIIDEIDGAPAVSINMLLSLINRKDAKETEGGTDVTTGKKKKKEGGLLLRPIICICNDQYVPSLRLLRQQAFMLNFPQTLPSRLVQRLYEITIKQGMKADTGALMALCEKTENDIRSCINTLQFLHGRGKKELNMRSVQTMKIGLKDQNKGLFSVWQEIFQLPKIQRKRIGQEIAIKDLHLFLGSGNESLGGVGKAPLSAVAQRFHHILHLSTSTGEYEKLTMGLYDNFLNMKVKESNFSTVCLALDWLEFTDIVNTTIMHGQNFQLMRYLPFLPVAFHLLFAASNVPRIAYPNSHYEAQSKLNQMQNLLNAMVAEISPAIRTRVGPQSLVLDALCLLLDVISPKLRPVNTQLFSTKEKQQLAELINTMLAYNLTYHQERTMEGQYVYKLDPNVEDVCRFPDLPIRKPLTYQAKQLIAREIELERMRRTEAFQQARNAGRDNASANAAKPTTETKGAKSTAKPAALNHEQRLENIMKKAAAFEEKPEKDFFGRQIVKKVAAPVAASANQEESVERRIGKAVGNSDVWFRFNEGVSNAVRRNIYIKDLL

Target ORF information:

RefSeq Version	XM_002935859.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chromosome transmission fidelity factor 18 (chtf18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935859.5

ORF Insert Sequence:

ATGGACGAAT ATGACTTGTA CGGAGTGGAG GATGATTTCG AGAGTCAGTT CGCCTCGGAG 
CTGGAAGTGC TGGCCGAGTT TGAGGATGAC GTACCTTCCC GTCCCGCTCC CCAAAGGTCC 
CAGCTACGCA GCAAACTTTC CTTCGAGGAG GCAATCTCTG CCGGGGATCG TGTAAAGAGC 
AACGCCGACA GCAAAACAAC TAGTGAGAGA AACGGCGATG CTCTCGCTTG CGTCAATACC 
AGCAAGACTA AAAAGCGAGA CGCAAGCTGC CTTGACTTTG AAGAGGATGA ATTCTCAGAC 
AGCTTCTTTG AGCCTCCTGT CACTCCCAAA CACAAACGGC CAAGAATTGA AGCTGTTAAG 
AAGCTAGACT TTGGAAGTGA TGAATATGCA GGCAACAGTA CTGCCCTCAG TGATGATATC 
ACACCTCCAC CCTCTCCTCA TTATACTCAG AAGAAAAGTG AAAGGGATTC CAAGTTCCCA 
AGCTGCGATG CCTTTGAGGT GAGCGACATG GCACCCCTGC AGGTAACGCC CACAGAATCG 
GAGCAGAAGC GCGTGCTTAA GCGTCCCCCT GTGCTGGAGG ATTACATTAA CGTGACATCC 
ACTGACGGGT CTAGGGTGTA TATGACATTA AAAGAGGATA ATGATGGCTC AGGGACTCAG 
CAAAAGCAAG GGAACGTGAA GTGGAACAGT GGTCAGCAGC TGCATCTCCT GGGGGTTCCA 
TTCTCCTACT TAAAGGAACA GGTTAACGAT GAGCGCCGAC GGCAGGTGTT AGAAGAATCC 
CAGAAACTTA CAGAAATGCT GAACAGTCAA ATTAATGATG AATTTGGAGA GGACGATAGT 
GAAACACTGG AAAATGGAGA TAATGCTGGA GAAGAGGATG GCGAAAATGA ATCTTCATCC 
CACTCGCTGT GGGTAGACTG CTTCACTCCC AGGCACTACA CTGAATTGCT CAGTGATGAT 
TACACAAACC GCTGCCTACT GAAATGGTTA AAGCTGTGGG ACACTGTGGT GTTTGGGAAA 
GAGAGAGTGG TGAAGAAACC CAAGGCAAAT GTGGACCCAC GCGCCAACTT CAAGAATCAG 
AAAGAGCAGC AGAGCAAGTT TAAGAGCAAA GCTCAGATCA CCGAGGAGAT CCTGGAAGCT 
GAACTGGATC AGCACAACAG ACCCAAAAAC AAGGTTGCAC TTCTTTGCGG CCCACCGGGC 
CTTGGAAAGA CCACACTGGC ACATGTAATA GCAAGGCACG CAGGATACAA TGTGGTGGAA 
ATGAATGCCA GTGATGATCG GAGCCCCGAG ATCTTTAAAA CCCGCATAGA AGCAGCGACT 
CAGATGAAAT CTGTTTTGGG TGTTGATGAG AGACCCAACT GCCTCATTAT AGATGAAATT 
GATGGTGCTC CAGCAGTCTC CATCAACATG CTGCTGAGTT TAATCAACCG AAAAGACGCC 
AAGGAAACTG AGGGTGGCAC AGATGTGACA ACAGGGAAGA AGAAAAAGAA AGAAGGTGGT 
TTACTGCTCA GGCCAATCAT CTGTATCTGC AATGATCAAT ATGTCCCTTC CTTACGGCTG 
CTGAGACAGC AAGCTTTCAT GCTGAATTTT CCTCAGACTC TGCCTTCCAG ACTGGTGCAA 
AGGCTGTATG AGATCACCAT TAAGCAAGGT ATGAAAGCAG ACACTGGGGC CCTCATGGCT 
TTGTGTGAAA AGACAGAGAA TGATATCAGA TCCTGTATTA ATACATTGCA GTTCCTACAC 
GGCCGAGGAA AGAAGGAGCT CAATATGCGC AGTGTGCAGA CCATGAAGAT CGGCCTGAAG 
GATCAAAACA AAGGCCTGTT CTCTGTTTGG CAGGAGATCT TCCAGCTTCC CAAGATTCAG 
AGGAAACGTA TCGGCCAAGA AATTGCAATT AAAGACCTCC ATCTTTTTTT GGGGTCAGGA 
AATGAATCTC TCGGTGGGGT GGGGAAGGCA CCATTAAGCG CCGTGGCTCA GAGATTTCAT 
CACATTCTGC ATCTCTCCAC TTCCACGGGA GAGTACGAGA AGCTCACCAT GGGTTTGTAC 
GACAATTTCC TAAACATGAA GGTGAAAGAG TCCAACTTCA GTACAGTGTG CCTGGCTTTG 
GATTGGCTGG AGTTCACGGA CATTGTGAAC ACGACTATAA TGCACGGGCA GAATTTCCAG 
CTCATGAGAT ACCTCCCCTT CCTGCCTGTA GCTTTCCATC TCCTGTTTGC AGCCTCAAAT 
GTGCCGCGCA TTGCATACCC CAACAGCCAC TATGAGGCAC AGTCCAAACT TAATCAAATG 
CAAAACCTAC TGAATGCAAT GGTGGCCGAA ATTTCTCCAG CTATCCGAAC GCGCGTGGGG 
CCTCAGTCCT TGGTGCTTGA TGCTCTGTGC CTTCTCCTGG ATGTGATCTC TCCTAAACTG 
CGCCCGGTAA ACACGCAGCT GTTCAGTACC AAAGAGAAGC AGCAGCTAGC CGAGCTTATT 
AATACTATGC TAGCCTATAA CCTGACCTAC CACCAGGAGC GGACCATGGA AGGGCAGTAT 
GTCTACAAGC TGGATCCGAA TGTTGAAGAT GTATGCAGGT TTCCAGATCT TCCAATCCGT 
AAACCATTGA CTTACCAAGC CAAACAGCTC ATAGCGCGCG AGATTGAACT GGAGAGAATG 
AGGAGGACAG AAGCATTCCA GCAAGCGAGA AATGCAGGAA GGGACAATGC CAGCGCCAAT 
GCCGCTAAAC CAACCACTGA AACCAAAGGT GCCAAAAGCA CGGCCAAACC CGCGGCTTTA 
AACCACGAAC AGCGGTTGGA GAATATCATG AAGAAGGCAG CAGCGTTTGA GGAGAAGCCT 
GAAAAAGATT TCTTTGGTCG CCAAATAGTC AAGAAGGTGG CAGCGCCAGT GGCAGCAAGT 
GCAAACCAGG AGGAATCGGT GGAGAGAAGA ATTGGGAAGG CGGTGGGAAA CAGCGATGTT 
TGGTTTCGCT TTAATGAGGG TGTTTCTAAT GCTGTGCGGC GGAATATATA CATTAAGGAC 
CTCCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chtf18 ( XM_002935859.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935905.2 Xenopus tropicalis chromosome transmission fidelity factor 18 (chtf18), mRNA.

Price & Availability↑

CloneID	OXa16479
Clone ID Related Accession (Same CDS sequence)	XM_002935859.4
Accession Version	XM_002935859.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	chromosome transmission fidelity protein 18 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935859.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAAT ATGACTTGTA CGGAGTGGAG GATGATTTCG AGAGTCAGTT CGCCTCGGAG 
CTGGAAGTGC TGGCCGAGTT TGAGGATGAC GTACCTTCCC GTCCCGCTCC CCAAAGGTCC 
CAGCTACGCA GCAAACTTTC CTTCGAGGAG GCAATCTCTG CCGGGGATCG TGTAAAGAGC 
AACGCCGACA GCAAAACAAC TAGTGAGAGA AACGGCGATG CTCTCGCTTG CGTCAATACC 
AGCAAGACTA AAAAGCGAGA CGCAAGCTGC CTTGACTTTG AAGAGGATGA ATTCTCAGAC 
AGCTTCTTTG AGCCTCCTGT CACTCCCAAA CACAAACGGC CAAGAATTGA AGCTGTTAAG 
AAGCTAGACT TTGGAAGTGA TGAATATGCA GGCAACAGTA CTGCCCTCAG TGATGATATC 
ACACCTCCAC CCTCTCCTCA TTATACTCAG AAGAAAAGTG AAAGGGATTC CAAGTTCCCA 
AGCTGCGATG CCTTTGAGGT GAGCGACATG GCACCCCTGC AGGTAACGCC CACAGAATCG 
GAGCAGAAGC GCGTGCTTAA GCGTCCCCCT GTGCTGGAGG ATTACATTAA CGTGACATCC 
ACTGACGGGT CTAGGGTGTA TATGACATTA AAAGAGGATA ATGATGGCTC AGGGACTCAG 
CAAAAGCAAG GGAACGTGAA GTGGAACAGT GGTCAGCAGC TGCATCTCCT GGGGGTTCCA 
TTCTCCTACT TAAAGGAACA GGTTAACGAT GAGCGCCGAC GGCAGGTGTT AGAAGAATCC 
CAGAAACTTA CAGAAATGCT GAACAGTCAA ATTAATGATG AATTTGGAGA GGACGATAGT 
GAAACACTGG AAAATGGAGA TAATGCTGGA GAAGAGGATG GCGAAAATGA ATCTTCATCC 
CACTCGCTGT GGGTAGACTG CTTCACTCCC AGGCACTACA CTGAATTGCT CAGTGATGAT 
TACACAAACC GCTGCCTACT GAAATGGTTA AAGCTGTGGG ACACTGTGGT GTTTGGGAAA 
GAGAGAGTGG TGAAGAAACC CAAGGCAAAT GTGGACCCAC GCGCCAACTT CAAGAATCAG 
AAAGAGCAGC AGAGCAAGTT TAAGAGCAAA GCTCAGATCA CCGAGGAGAT CCTGGAAGCT 
GAACTGGATC AGCACAACAG ACCCAAAAAC AAGGTTGCAC TTCTTTGCGG CCCACCGGGC 
CTTGGAAAGA CCACACTGGC ACATGTAATA GCAAGGCACG CAGGATACAA TGTGGTGGAA 
ATGAATGCCA GTGATGATCG GAGCCCCGAG ATCTTTAAAA CCCGCATAGA AGCAGCGACT 
CAGATGAAAT CTGTTTTGGG TGTTGATGAG AGACCCAACT GCCTCATTAT AGATGAAATT 
GATGGTGCTC CAACAGTCTC CATCAACATG CTGCTGAGTT TAATCAACCG AAAAGACGCC 
AAGGAAACTG AGGGTGGCAC AGATGTGACA ACAGGGAAGA AGAAAAAGAA AGAAGGTGGT 
TTACTGCTCA GGCCAATCAT CTGTATCTGC AATGATCAAT ATGTCCCTTC CTTACGGCTG 
CTGAGACAGC AAGCTTTCAT GCTGAATTTT CCTCAGACTC TGCCTTCCAG ACTGGTGCAA 
AGGCTGTATG AGATCACCAT TAAGCAAGGT ATGAAAGCAG ACACTGGGGC CCTCATGGCT 
TTGTGTGAAA AGACAGAGAA TGATATCAGA TCCTGTATTA ATACATTGCA GTTCCTACAC 
GGCCGAGGAA AGAAGGAGCT CAATATGCGC AGTGTGCAGA CCATGAAGAT CGGCCTGAAG 
GATCAAAACA AAGGCCTGTT CTCTGTTTGG CAGGAGATCT TCCAGCTTCC CAAGATTCAG 
AGGAAACGTA TCGGCCAAGA AATTGCAATT AAAGACCTCC ATCTTTTTTT GGGGTCAGGA 
AATGAATCTC TCGGTGGGGT GGGGAAGGCA CCATTAAGCG CCGTGGCTCA GAGATTTCAT 
CACATTCTGC ATCTCTCCAC TTCCACGGGA GAGTACGAGA AGCTCACCAT GGGTTTGTAC 
GACAATTTCC TAAACATGAA GGTGAAAGAG TCCAACTTTA GTACAGTGTG CCTGGCTTTG 
GATTGGCTGG AGTTCACGGA CATTGTGAAC ACGACTATAA TGCACGGGCA GAATTTCCAG 
CTCATGAGAT ACCTCCCCTT CCTGCCTGTA GCTTTCCATC TCCTGTTTGC AGCCTCAAAT 
GTGCCGCGCA TTGCATACCC CAACAGCCAC TATGAGGCAC AGTCCAAACT TAATCAAATG 
CAAAACCTAC TGAATGCAAT GGTGGCCGAA ATTTCTCCAG CTATCCGAAC GCGCGTGGGG 
CCTCAGTCCT TGGTGCTTGA TGCTCTGTGC CTTCTCCTGG ATGTGATCTC TCCTAAACTG 
CGCCCGGTAA ACACGCAGCT GTTCAGTACC AAAGAGAAGC AGCAGCTAGC CGAGCTTATT 
AATACTATGC TAGCCTATAA CCTGACCTAC CACCAGGAGC GGACCATGGA AGGGCAGTAT 
GTCTACAAGC TGGATCCGAA TGTTGAAGAT GTATGCAGGT TTCCAGATCT TCCAATCCGT 
AAGCCATTGA CTTACCAAGC CAAACAGCTC ATAGCGCGCG AGATTGAACT GGAGAGAATG 
AGGAGGACAG AAGCATTCCA GCAAGCGAGA AATGCAGGAA GGGACAATGC CAGCGCCAAT 
GCCGCTAAAC CAACCACTGA AACCAAAGGT GCCAAAAGCA CGGCCAAACC CGCGGCTTTA 
AACCACGAAC AGCGGTTGGA GAATATCATG AAGAAGGCAG CAGCGTTTGA GGAGAAGCCT 
GAAAAAGATT TCTTTGGTCG CCAAATAGTC AAGAAGGTGG CAGCGCCAGT GGCAGCAAGT 
GCAAACCAGG AGGAATCGGT GGAGAGAAGA ATTGGGAAGG CGGTGGGAAA CAGCGATGTT 
TGGTTTCGCT TTAATGAGGG TGTTTCTAAT GCTGTGCGGC GGAATATATA CATTAAGGAC 
CTCCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935905.2
CDS	174..3182
Translation	MDEYDLYGVEDDFESQFASELEVLAEFEDDVPSRPAPQRSQLRSKLSFEEAISAGDRVKSNADSKTTSERNGDALACVNTSKTKKRDASCLDFEEDEFSDSFFEPPVTPKHKRPRIEAVKKLDFGSDEYAGNSTALSDDITPPPSPHYTQKKSERDSKFPSCDAFEVSDMAPLQVTPTESEQKRVLKRPPVLEDYINVTSTDGSRVYMTLKEDNDGSGTQQKQGNVKWNSGQQLHLLGVPFSYLKEQVNDERRRQVLEESQKLTEMLNSQINDEFGEDDSETLENGDNAGEEDGENESSSHSLWVDCFTPRHYTELLSDDYTNRCLLKWLKLWDTVVFGKERVVKKPKANVDPRANFKNQKEQQSKFKSKAQITEEILEAELDQHNRPKNKVALLCGPPGLGKTTLAHVIARHAGYNVVEMNASDDRSPEIFKTRIEAATQMKSVLGVDERPNCLIIDEIDGAPTVSINMLLSLINRKDAKETEGGTDVTTGKKKKKEGGLLLRPIICICNDQYVPSLRLLRQQAFMLNFPQTLPSRLVQRLYEITIKQGMKADTGALMALCEKTENDIRSCINTLQFLHGRGKKELNMRSVQTMKIGLKDQNKGLFSVWQEIFQLPKIQRKRIGQEIAIKDLHLFLGSGNESLGGVGKAPLSAVAQRFHHILHLSTSTGEYEKLTMGLYDNFLNMKVKESNFSTVCLALDWLEFTDIVNTTIMHGQNFQLMRYLPFLPVAFHLLFAASNVPRIAYPNSHYEAQSKLNQMQNLLNAMVAEISPAIRTRVGPQSLVLDALCLLLDVISPKLRPVNTQLFSTKEKQQLAELINTMLAYNLTYHQERTMEGQYVYKLDPNVEDVCRFPDLPIRKPLTYQAKQLIAREIELERMRRTEAFQQARNAGRDNASANAAKPTTETKGAKSTAKPAALNHEQRLENIMKKAAAFEEKPEKDFFGRQIVKKVAAPVAASANQEESVERRIGKAVGNSDVWFRFNEGVSNAVRRNIYIKDLL

Target ORF information:

RefSeq Version	XM_002935859.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis chromosome transmission fidelity factor 18 (chtf18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935859.4

ORF Insert Sequence:

ATGGACGAAT ATGACTTGTA CGGAGTGGAG GATGATTTCG AGAGTCAGTT CGCCTCGGAG 
CTGGAAGTGC TGGCCGAGTT TGAGGATGAC GTACCTTCCC GTCCCGCTCC CCAAAGGTCC 
CAGCTACGCA GCAAACTTTC CTTCGAGGAG GCAATCTCTG CCGGGGATCG TGTAAAGAGC 
AACGCCGACA GCAAAACAAC TAGTGAGAGA AACGGCGATG CTCTCGCTTG CGTCAATACC 
AGCAAGACTA AAAAGCGAGA CGCAAGCTGC CTTGACTTTG AAGAGGATGA ATTCTCAGAC 
AGCTTCTTTG AGCCTCCTGT CACTCCCAAA CACAAACGGC CAAGAATTGA AGCTGTTAAG 
AAGCTAGACT TTGGAAGTGA TGAATATGCA GGCAACAGTA CTGCCCTCAG TGATGATATC 
ACACCTCCAC CCTCTCCTCA TTATACTCAG AAGAAAAGTG AAAGGGATTC CAAGTTCCCA 
AGCTGCGATG CCTTTGAGGT GAGCGACATG GCACCCCTGC AGGTAACGCC CACAGAATCG 
GAGCAGAAGC GCGTGCTTAA GCGTCCCCCT GTGCTGGAGG ATTACATTAA CGTGACATCC 
ACTGACGGGT CTAGGGTGTA TATGACATTA AAAGAGGATA ATGATGGCTC AGGGACTCAG 
CAAAAGCAAG GGAACGTGAA GTGGAACAGT GGTCAGCAGC TGCATCTCCT GGGGGTTCCA 
TTCTCCTACT TAAAGGAACA GGTTAACGAT GAGCGCCGAC GGCAGGTGTT AGAAGAATCC 
CAGAAACTTA CAGAAATGCT GAACAGTCAA ATTAATGATG AATTTGGAGA GGACGATAGT 
GAAACACTGG AAAATGGAGA TAATGCTGGA GAAGAGGATG GCGAAAATGA ATCTTCATCC 
CACTCGCTGT GGGTAGACTG CTTCACTCCC AGGCACTACA CTGAATTGCT CAGTGATGAT 
TACACAAACC GCTGCCTACT GAAATGGTTA AAGCTGTGGG ACACTGTGGT GTTTGGGAAA 
GAGAGAGTGG TGAAGAAACC CAAGGCAAAT GTGGACCCAC GCGCCAACTT CAAGAATCAG 
AAAGAGCAGC AGAGCAAGTT TAAGAGCAAA GCTCAGATCA CCGAGGAGAT CCTGGAAGCT 
GAACTGGATC AGCACAACAG ACCCAAAAAC AAGGTTGCAC TTCTTTGCGG CCCACCGGGC 
CTTGGAAAGA CCACACTGGC ACATGTAATA GCAAGGCACG CAGGATACAA TGTGGTGGAA 
ATGAATGCCA GTGATGATCG GAGCCCCGAG ATCTTTAAAA CCCGCATAGA AGCAGCGACT 
CAGATGAAAT CTGTTTTGGG TGTTGATGAG AGACCCAACT GCCTCATTAT AGATGAAATT 
GATGGTGCTC CAACAGTCTC CATCAACATG CTGCTGAGTT TAATCAACCG AAAAGACGCC 
AAGGAAACTG AGGGTGGCAC AGATGTGACA ACAGGGAAGA AGAAAAAGAA AGAAGGTGGT 
TTACTGCTCA GGCCAATCAT CTGTATCTGC AATGATCAAT ATGTCCCTTC CTTACGGCTG 
CTGAGACAGC AAGCTTTCAT GCTGAATTTT CCTCAGACTC TGCCTTCCAG ACTGGTGCAA 
AGGCTGTATG AGATCACCAT TAAGCAAGGT ATGAAAGCAG ACACTGGGGC CCTCATGGCT 
TTGTGTGAAA AGACAGAGAA TGATATCAGA TCCTGTATTA ATACATTGCA GTTCCTACAC 
GGCCGAGGAA AGAAGGAGCT CAATATGCGC AGTGTGCAGA CCATGAAGAT CGGCCTGAAG 
GATCAAAACA AAGGCCTGTT CTCTGTTTGG CAGGAGATCT TCCAGCTTCC CAAGATTCAG 
AGGAAACGTA TCGGCCAAGA AATTGCAATT AAAGACCTCC ATCTTTTTTT GGGGTCAGGA 
AATGAATCTC TCGGTGGGGT GGGGAAGGCA CCATTAAGCG CCGTGGCTCA GAGATTTCAT 
CACATTCTGC ATCTCTCCAC TTCCACGGGA GAGTACGAGA AGCTCACCAT GGGTTTGTAC 
GACAATTTCC TAAACATGAA GGTGAAAGAG TCCAACTTTA GTACAGTGTG CCTGGCTTTG 
GATTGGCTGG AGTTCACGGA CATTGTGAAC ACGACTATAA TGCACGGGCA GAATTTCCAG 
CTCATGAGAT ACCTCCCCTT CCTGCCTGTA GCTTTCCATC TCCTGTTTGC AGCCTCAAAT 
GTGCCGCGCA TTGCATACCC CAACAGCCAC TATGAGGCAC AGTCCAAACT TAATCAAATG 
CAAAACCTAC TGAATGCAAT GGTGGCCGAA ATTTCTCCAG CTATCCGAAC GCGCGTGGGG 
CCTCAGTCCT TGGTGCTTGA TGCTCTGTGC CTTCTCCTGG ATGTGATCTC TCCTAAACTG 
CGCCCGGTAA ACACGCAGCT GTTCAGTACC AAAGAGAAGC AGCAGCTAGC CGAGCTTATT 
AATACTATGC TAGCCTATAA CCTGACCTAC CACCAGGAGC GGACCATGGA AGGGCAGTAT 
GTCTACAAGC TGGATCCGAA TGTTGAAGAT GTATGCAGGT TTCCAGATCT TCCAATCCGT 
AAGCCATTGA CTTACCAAGC CAAACAGCTC ATAGCGCGCG AGATTGAACT GGAGAGAATG 
AGGAGGACAG AAGCATTCCA GCAAGCGAGA AATGCAGGAA GGGACAATGC CAGCGCCAAT 
GCCGCTAAAC CAACCACTGA AACCAAAGGT GCCAAAAGCA CGGCCAAACC CGCGGCTTTA 
AACCACGAAC AGCGGTTGGA GAATATCATG AAGAAGGCAG CAGCGTTTGA GGAGAAGCCT 
GAAAAAGATT TCTTTGGTCG CCAAATAGTC AAGAAGGTGG CAGCGCCAGT GGCAGCAAGT 
GCAAACCAGG AGGAATCGGT GGAGAGAAGA ATTGGGAAGG CGGTGGGAAA CAGCGATGTT 
TGGTTTCGCT TTAATGAGGG TGTTTCTAAT GCTGTGCGGC GGAATATATA CATTAAGGAC 
CTCCTATAG