usp53 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp53
Entrez Gene ID	100158512
Full Name	ubiquitin specific peptidase 53
General protein information	Preferred Names ubiquitin specific peptidase 53 Names inactive ubiquitin carboxyl-terminal hydrolase 53
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002934255.4	XP_002934301.1	inactive ubiquitin carboxyl-terminal hydrolase 53
XM_002934255.5	XP_002934301.2	inactive ubiquitin carboxyl-terminal hydrolase 53

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP53	NP_061923.2
Gallus gallus (chicken)	USP53	XP_420633.4
Canis lupus familiaris (dog)	USP53	XP_545046.3
Bos taurus (cattle)	USP53	XP_003586226.1
Mus musculus (house mouse)	Usp53	NP_598618.1
Xenopus tropicalis (tropical clawed frog)	usp53	XP_002934301.1
Pan troglodytes (chimpanzee)	USP53	XP_517410.2
	USP53	XP_001098826.2

Gene Ontology

The following usp53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa48189	XM_002934255.5 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 53 (usp53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa04535	XM_002934255.4 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 53 (usp53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp53 ( XM_002934255.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934301.2 Xenopus tropicalis ubiquitin specific peptidase 53 (usp53), mRNA.

Price & Availability↑

CloneID	OXa48189
Clone ID Related Accession (Same CDS sequence)	XM_002934255.5
Accession Version	XM_002934255.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934255.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGGG TGAAGTTTTT TCGGCGTCAG GCAGGAGGGA ACCTAGGGAA AGTGTACCAG 
CCTGGCAGCG TCCTCTCTCT GGCACCTACC AAGGGCCTGT TCAATGAGCC CGGGCAGAAC 
AGCTGCTTCC TGAACAGCGC CGTGCAGGTT CTGTGGCAAC TGGACATTTT TCGACGCAGT 
TTACGCGGAC TGACTGGACA TGTTTGCTTA GGAGATGCTT GTATATTTTG TGCATTAAAG 
TCCATATTTG CACAGTTTCA AACAAGCCGA GAGAAGGTTC TACCATCGGA TGCTATGAGA 
CATGCTCTTG CAGAAAGCTT CAAAGATGAG CATCGCTTTC AGCTTGGCCT TATGGATGAT 
GCTGCTGAAT GTTTTGAAAA CATATTGCAG CGGATTCATT TCCATATTGT GCCAAATAGT 
GAAGGAGATA TGTGCACTTC AAAATCTTGT ATCACCCACC AGAAGTTTGC AATGACTCTT 
TATGAACAGT GTGTTTGCCG CAGCTGTGGA GCTTCCTCTG ATCCATTGCC TTTTACTGAG 
TTTGTGAGAT ACATTTCTAC AACAGCACTT TGTAATGAAG TTGACCGAAT GCTAGAGAGG 
CATGAACGCT TGAAGCCAGA AATGTTTGCC GATTTACTTC AGGCTGCTAA CACCACAGAT 
GATTACAGGA AGTGTCCGAG TAACTGTGGC CAGAGAATCA AAATCCGGCG TGTACTTATG 
AACTGCCCAG AGATTGTTAC TATTGGCCTG GTTTGGGATT CTGATAACTC TGACTTAACT 
GAAGATGTTG TGCGAAACTT GGCTACTCAG CTTTATTTAC CCGGGCTGTT TTTCAGGGTT 
ACTGATGAAC AAGCCAAAGA AAGTAGACTT TACCTTGTCG GTATGATCTG CTACACGAGT 
AAACACTACT GTGCCTTTGC CTATCACACA AAGAGTGCCA AGTGGTTTTT GTTTGATGAT 
GCATCCGTAA AAGAGGTTGG TACAAAGTGG AAAGATGTGG TCACCAAGTG CATTCGGTGT 
CATTACCAGC CTTTGCTTCT GTTTTATGCA AATCCTGATG GCACGGCAGT AACTAATGAG 
GATGCACTAA GACAGGTTCA GCATTGGTCT CACTGTAAAA GAGAGTTACA TAATGAGGAG 
ACAGGTACTG AAAGATCATC CTCTGTACCA AAGAAGCCAG ATTACAGCAG AGATCATGAG 
TTTGGTGAAC ACATATATCA GAACAATGGG TACAAATTCC ATATGGAAGA ACCAGCTTTT 
AATAGAGGAG TTGTTCATTC GAGTGCTGGC AGAGGGCCTG TTAAACTAAA TCGTAATGAA 
CAACAAGAAA AACTGAGGGA TGTTTCTAGA GAATGTGCAC AGAAAGCAAA CATGAAAAAT 
TTATCATCCT CACAAAGAAA AGTTACTGAG AAAACACAGA AGAAAGAAAT CATCAGACCC 
AAGGAGACTT CAGGAGGAAT GGCATACAAT TCAAAGCCTA GCACAGTAGT GCATCTCAAG 
TCTGAATCCT CCCCCTCTTT TAATGGCTTT AGGAAGTATC CTGATGCTCA TTTCTTTAGT 
AGTCAAGGGA AAGGTCCGTA CAAGCAAGAA AGACCAACGA GCCAGCCTAG ACAATCTTCT 
TCTTTTAATG CAGGGCATGC CACAATTCCT GAATCCAAGC AAGTGGCCAG GGTGAAAAAC 
AATTTTTCAG CTGGTTATGA TACAGACAGT AGTCAGGAAT ACAGAGAAAA ACCAAACTCC 
AGAAATAAAA AAACTTGGAA GCCTATGCGG GAGACACTTA ATGTGGATAG CATTTTCAAT 
GACACTGATA AAAGCCAACA TAGCCCAAGA CATTTAGTAG GCTCAAATGG AAAACAATCT 
GCCAGGGATC ATAGTTTTAA CCCACCATCA AAAGAGAGCC CAAAACGTAA TACTCTGATG 
ACTATCTTTG AGGATGAGAT AAAACAGGAA TCTGGCAGCA GGAGTTCCTT GGAATCATTA 
GGAAAAAGTC AAGCAGATCG GAACAAAGTT ACTGCAGATA TGAAGATGTA CCACCTTGAC 
AACTGGCAGA GAACTGAATC TGGATATGAA AGTAGCGATC ACATCAGCAG CGGGTCAAGC 
AATCTGGATT CACCTATTAT TGAAGGGATT GGTTTTGCTG ATGGGAGGGG CTTGCAAGAA 
ACCAGCCTGT GCAGTGATCA GCAGCTGTCA AATGCACTGT TATCTACCTC ACCACAAACC 
AGAAGTAATC TGAATGGTGC AAACAATGAA CACCATGCTC TGGAGCACTG CAAATCTGAA 
GACAGGCTTT CTATTGTTAC CCGGGAACCT GCACACAGCC CGTCAGGTCT CAGGAAGCAA 
TTTGTTGAAT TGAGAAGAGA CCTACGCTCA CCAAACCTGT TGTCAGCATC AAACGATGAT 
GTGAAGGAAT CGGAGGATAG TCTGGAAAGT TCTAGCTGGT TGAATTCTTG TCCCTCGGAC 
AACTGTCGTT TGCACCATGA AAATATTCAC TCGTTACGAG AAATTTCAAC AGGCCCAGCA 
AAAATGCTGA ATAATGGATT GTGTTCCCAG CGACAGCATG CAGCTAAAAC CTCTTCACAG 
CTGGCCTCTC CGAGCAACAG GCTGCAGTAT ATGAATTCTC AGTTCTTCGT ATTCAATGAG 
GATCAGTCAT GCAGCAGAGA TGAGCGCTGC TCTGTAGATC CTCCTGAAGT ACCTCCCCCT 
CTACCCCCCA AAAAATATGT AAGAAATGCG GCACAAAGAA CTGAACAAAG CTTTGCATCC 
ACAATCAGCA GTAAACTTTC TGAAACATTA GCACGAGCTT ATCCTGGAAT CTCTGAGAGA 
TATTTATCTG GGGATTTCAT TAGATCTGAG CCTGATAGTG GCTATTATCC AAAGGAAGAA 
TCATTTCATG GTACCGAAAT TAATACTAAA CCCGTCAACC TGGCTGGTTC TTCCTCTTAT 
GGCTGTCAGT CAAAGATAAA CTCACACACT ATTGGTAGAG ATGTTGCCCC ACATATAAAT 
GATACTATTG CTCCCACTAC TTACTTCTCA GTTGATAACT GTATGACAGA CACATACAGG 
ATGAAATATC ACCACAGGCC CAGAATGAAT ATGGGAGACC GTTCTGGGTA CCAGAGAGAT 
CCTGGTCATC TGCAGCTAAC GGATAAATTA ATGGAACCAG CATACCATAG AACTTTAGAA 
AATGCTTCAC GTTCCAAACA AACTTTGGGA GGTATACCAT GTAATCGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934301.2
CDS	555..3785
Translation	MAWVKFFRRQAGGNLGKVYQPGSVLSLAPTKGLFNEPGQNSCFLNSAVQVLWQLDIFRRSLRGLTGHVCLGDACIFCALKSIFAQFQTSREKVLPSDAMRHALAESFKDEHRFQLGLMDDAAECFENILQRIHFHIVPNSEGDMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVDRMLERHERLKPEMFADLLQAANTTDDYRKCPSNCGQRIKIRRVLMNCPEIVTIGLVWDSDNSDLTEDVVRNLATQLYLPGLFFRVTDEQAKESRLYLVGMICYTSKHYCAFAYHTKSAKWFLFDDASVKEVGTKWKDVVTKCIRCHYQPLLLFYANPDGTAVTNEDALRQVQHWSHCKRELHNEETGTERSSSVPKKPDYSRDHEFGEHIYQNNGYKFHMEEPAFNRGVVHSSAGRGPVKLNRNEQQEKLRDVSRECAQKANMKNLSSSQRKVTEKTQKKEIIRPKETSGGMAYNSKPSTVVHLKSESSPSFNGFRKYPDAHFFSSQGKGPYKQERPTSQPRQSSSFNAGHATIPESKQVARVKNNFSAGYDTDSSQEYREKPNSRNKKTWKPMRETLNVDSIFNDTDKSQHSPRHLVGSNGKQSARDHSFNPPSKESPKRNTLMTIFEDEIKQESGSRSSLESLGKSQADRNKVTADMKMYHLDNWQRTESGYESSDHISSGSSNLDSPIIEGIGFADGRGLQETSLCSDQQLSNALLSTSPQTRSNLNGANNEHHALEHCKSEDRLSIVTREPAHSPSGLRKQFVELRRDLRSPNLLSASNDDVKESEDSLESSSWLNSCPSDNCRLHHENIHSLREISTGPAKMLNNGLCSQRQHAAKTSSQLASPSNRLQYMNSQFFVFNEDQSCSRDERCSVDPPEVPPPLPPKKYVRNAAQRTEQSFASTISSKLSETLARAYPGISERYLSGDFIRSEPDSGYYPKEESFHGTEINTKPVNLAGSSSYGCQSKINSHTIGRDVAPHINDTIAPTTYFSVDNCMTDTYRMKYHHRPRMNMGDRSGYQRDPGHLQLTDKLMEPAYHRTLENASRSKQTLGGIPCNR

Target ORF information:

RefSeq Version	XM_002934255.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 53 (usp53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934255.5

ORF Insert Sequence:

ATGGCCTGGG TGAAGTTTTT TCGGCGTCAG GCAGGAGGGA ACCTAGGGAA AGTGTACCAG 
CCTGGCAGCG TCCTCTCTCT GGCACCTACC AAGGGCCTGT TCAATGAGCC CGGGCAGAAC 
AGCTGCTTCC TGAACAGCGC CGTGCAGGTT CTGTGGCAAC TGGACATTTT TCGACGCAGT 
TTACGCGGAC TGACTGGACA TGTTTGCTTA GGAGATGCTT GTATATTTTG TGCATTAAAG 
TCCATATTTG CACAGTTTCA AACAAGCCGA GAGAAGGTTC TACCATCGGA TGCTATGAGA 
CATGCTCTTG CAGAAAGCTT CAAAGATGAG CATCGCTTTC AGCTTGGCCT TATGGATGAT 
GCTGCTGAAT GTTTTGAAAA CATATTGCAG CGGATTCATT TCCATATTGT GCCAAATAGT 
GAAGGAGATA TGTGCACTTC AAAATCTTGT ATCACCCACC AGAAGTTTGC AATGACTCTT 
TATGAACAGT GTGTTTGCCG CAGCTGTGGA GCTTCCTCTG ATCCATTGCC TTTTACTGAG 
TTTGTGAGAT ACATTTCTAC AACAGCACTT TGTAATGAAG TTGACCGAAT GCTAGAGAGG 
CATGAACGCT TGAAGCCAGA AATGTTTGCC GATTTACTTC AGGCTGCTAA CACCACAGAT 
GATTACAGGA AGTGTCCGAG TAACTGTGGC CAGAGAATCA AAATCCGGCG TGTACTTATG 
AACTGCCCAG AGATTGTTAC TATTGGCCTG GTTTGGGATT CTGATAACTC TGACTTAACT 
GAAGATGTTG TGCGAAACTT GGCTACTCAG CTTTATTTAC CCGGGCTGTT TTTCAGGGTT 
ACTGATGAAC AAGCCAAAGA AAGTAGACTT TACCTTGTCG GTATGATCTG CTACACGAGT 
AAACACTACT GTGCCTTTGC CTATCACACA AAGAGTGCCA AGTGGTTTTT GTTTGATGAT 
GCATCCGTAA AAGAGGTTGG TACAAAGTGG AAAGATGTGG TCACCAAGTG CATTCGGTGT 
CATTACCAGC CTTTGCTTCT GTTTTATGCA AATCCTGATG GCACGGCAGT AACTAATGAG 
GATGCACTAA GACAGGTTCA GCATTGGTCT CACTGTAAAA GAGAGTTACA TAATGAGGAG 
ACAGGTACTG AAAGATCATC CTCTGTACCA AAGAAGCCAG ATTACAGCAG AGATCATGAG 
TTTGGTGAAC ACATATATCA GAACAATGGG TACAAATTCC ATATGGAAGA ACCAGCTTTT 
AATAGAGGAG TTGTTCATTC GAGTGCTGGC AGAGGGCCTG TTAAACTAAA TCGTAATGAA 
CAACAAGAAA AACTGAGGGA TGTTTCTAGA GAATGTGCAC AGAAAGCAAA CATGAAAAAT 
TTATCATCCT CACAAAGAAA AGTTACTGAG AAAACACAGA AGAAAGAAAT CATCAGACCC 
AAGGAGACTT CAGGAGGAAT GGCATACAAT TCAAAGCCTA GCACAGTAGT GCATCTCAAG 
TCTGAATCCT CCCCCTCTTT TAATGGCTTT AGGAAGTATC CTGATGCTCA TTTCTTTAGT 
AGTCAAGGGA AAGGTCCGTA CAAGCAAGAA AGACCAACGA GCCAGCCTAG ACAATCTTCT 
TCTTTTAATG CAGGGCATGC CACAATTCCT GAATCCAAGC AAGTGGCCAG GGTGAAAAAC 
AATTTTTCAG CTGGTTATGA TACAGACAGT AGTCAGGAAT ACAGAGAAAA ACCAAACTCC 
AGAAATAAAA AAACTTGGAA GCCTATGCGG GAGACACTTA ATGTGGATAG CATTTTCAAT 
GACACTGATA AAAGCCAACA TAGCCCAAGA CATTTAGTAG GCTCAAATGG AAAACAATCT 
GCCAGGGATC ATAGTTTTAA CCCACCATCA AAAGAGAGCC CAAAACGTAA TACTCTGATG 
ACTATCTTTG AGGATGAGAT AAAACAGGAA TCTGGCAGCA GGAGTTCCTT GGAATCATTA 
GGAAAAAGTC AAGCAGATCG GAACAAAGTT ACTGCAGATA TGAAGATGTA CCACCTTGAC 
AACTGGCAGA GAACTGAATC TGGATATGAA AGTAGCGATC ACATCAGCAG CGGGTCAAGC 
AATCTGGATT CACCTATTAT TGAAGGGATT GGTTTTGCTG ATGGGAGGGG CTTGCAAGAA 
ACCAGCCTGT GCAGTGATCA GCAGCTGTCA AATGCACTGT TATCTACCTC ACCACAAACC 
AGAAGTAATC TGAATGGTGC AAACAATGAA CACCATGCTC TGGAGCACTG CAAATCTGAA 
GACAGGCTTT CTATTGTTAC CCGGGAACCT GCACACAGCC CGTCAGGTCT CAGGAAGCAA 
TTTGTTGAAT TGAGAAGAGA CCTACGCTCA CCAAACCTGT TGTCAGCATC AAACGATGAT 
GTGAAGGAAT CGGAGGATAG TCTGGAAAGT TCTAGCTGGT TGAATTCTTG TCCCTCGGAC 
AACTGTCGTT TGCACCATGA AAATATTCAC TCGTTACGAG AAATTTCAAC AGGCCCAGCA 
AAAATGCTGA ATAATGGATT GTGTTCCCAG CGACAGCATG CAGCTAAAAC CTCTTCACAG 
CTGGCCTCTC CGAGCAACAG GCTGCAGTAT ATGAATTCTC AGTTCTTCGT ATTCAATGAG 
GATCAGTCAT GCAGCAGAGA TGAGCGCTGC TCTGTAGATC CTCCTGAAGT ACCTCCCCCT 
CTACCCCCCA AAAAATATGT AAGAAATGCG GCACAAAGAA CTGAACAAAG CTTTGCATCC 
ACAATCAGCA GTAAACTTTC TGAAACATTA GCACGAGCTT ATCCTGGAAT CTCTGAGAGA 
TATTTATCTG GGGATTTCAT TAGATCTGAG CCTGATAGTG GCTATTATCC AAAGGAAGAA 
TCATTTCATG GTACCGAAAT TAATACTAAA CCCGTCAACC TGGCTGGTTC TTCCTCTTAT 
GGCTGTCAGT CAAAGATAAA CTCACACACT ATTGGTAGAG ATGTTGCCCC ACATATAAAT 
GATACTATTG CTCCCACTAC TTACTTCTCA GTTGATAACT GTATGACAGA CACATACAGG 
ATGAAATATC ACCACAGGCC CAGAATGAAT ATGGGAGACC GTTCTGGGTA CCAGAGAGAT 
CCTGGTCATC TGCAGCTAAC GGATAAATTA ATGGAACCAG CATACCATAG AACTTTAGAA 
AATGCTTCAC GTTCCAAACA AACTTTGGGA GGTATACCAT GTAATCGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp53 ( XM_002934255.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934301.1 Xenopus tropicalis ubiquitin specific peptidase 53 (usp53), mRNA.

Price & Availability↑

CloneID	OXa04535
Clone ID Related Accession (Same CDS sequence)	XM_002934255.4
Accession Version	XM_002934255.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934255.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGGG TGAAGCTTTT TCGGCGTCAG GCAGGAGGGA ACCTAGGGAA AGTGTACCAG 
CCTGGCAGCG TCCTCTCTCT GGCACCTACC AAGGGCCTGT TCAATGAGCC CGGGCAGAAC 
AGCTGCTTCC TGAACAGCGC CGTGCAGGTT CTGTGGCAAC TGGACATTTT TCGACGCAGT 
TTACGCGGAC TGACTGGACA TGTTTGCTTA GGAGATGCTT GTATATTTTG TGCATTAAAG 
TCCATATTTG CACAGTTTCA AACAAGCCGA GAGAAGGTTC TACCATCGGA TGCTATGAGA 
CATGCTCTTG CAGAAAGCTT CAAAGATGAG CATCGCTTTC AGCTTGGCCT TATGGATGAT 
GCTGCTGAAT GTTTTGAAAA CATATTGCAG CGGATTCATT TCCATATTGT GCCAAATAGT 
GAAGGAGATA TGTGCACTTC AAAATCTTGT ATCACCCACC AGAAGTTTGC AATGACTCTT 
TATGAACAGT GTGTTTGCCG CAGCTGTGGA GCTTCCTCTG ATCCATTGCC TTTTACTGAG 
TTTGTGAGAT ACATTTCTAC AACAGCACTT TGTAATGAAG TTGACCGAAT GCTAGAGAGG 
CATGAACGCT TGAAGCCAGA AATGTTTGCC GATTTACTTC AGGCTGCTAA CACCACAGAT 
GATTACAGGA AGTGTCCGAG TAACTGTGGC CAGAGAATCA AAATCCGGCG TGTACTTATG 
AACTGCCCAG AGATTGTTAC TATTGGCCTG GTTTGGGATT CTGATAACTC TGACTTAACT 
GAAGATGTTG TGCGAAACTT GGCTACTCAG CTTTATTTAC CCGGGCTGTT TTTCAGGGTT 
ACTGATGAAC AAGCCAAAGA AAGTAGACTT TACCTTGTCG GTATGATCTG CTACACGAGT 
AAACACTACT GTGCCTTTGC CTATCACACA AAGAGTGCCA AGTGGTTTTT GTTTGATGAT 
GCATCCGTAA AAGAGGTTGG TACAAAGTGG AAAGATGTGG TCACCAAGTG CATTCGGTGT 
CATTACCAGC CTTTGCTTCT GTTTTATGCA AATCCTGATG GCACGGCAGT AACTAATGAG 
GATGCACTAA GACAGGTTCA GCATTGGTCT CACTGTAAAA GAGAGTTACA TAATGAGGAG 
ACAGGTACTG AAAGATCATC CTCTGTACCA AAGAAGCCAG ATTACAGCAG AGATCATGAG 
TTTGGTGAAC ACATATATCA GAACAATGGG TACAAATTCC ATATGGAAGA ACCAGCTTTT 
AATAGAGGAG TTGTTCATTC GAGTGCTGGC AGAGGGCCTG TTAAACTAAA TCGTAATGAA 
CAACAAGAAA AACTGAGGGA TGTTTCTAGA GAATGTGCAC AGAAAGCAAA CATGAAAAAT 
TTATCATCCT CACAAAGAAA AGTTACTGAG AAAACACAGA AGAAAGAAAT CATCAGACCC 
AAGGAGACTT CAGGAGGAAT GGCATACAAT TCAAAGCCTA GCACAGTAGT GCATCTCAAG 
TCTGAATCCT CCCCCTCTTT TAATGGCTTT AGGAAGTATC CTGATGCTCA TTTCTTTAGT 
AGTCAAGGGA AAGGTCCGTA CAAGCAAGAA AGACCAACGA GCCAGCCTAG ACAATCTTCT 
TCTTTTAATG CAGGGCATGC CACAATTCCT GAATCCAAGC AAGTGGCCAG GGTGAAAAAC 
AATTTTTCAG CTGGTTATGA TACAGACAGT AGTCAGGAAT ACAGAGAAAA ACCAAACTCC 
AGAAATAAAA AAACTTGGAA GCCTATGCGG GAGACACTTA ATGTGGATAG CATTTTCAAT 
GACACTGATA AAAGCCAACA TAGCCCAAGA CATTTAGTAG GCTCAAATGG AAAACAATCT 
GCCAGGGATC ATAGTTTTAA CCCACCATCA AAAGAGAGCC CAAAACGTAA TACTCTGATG 
ACTATCTTTG AGGATGAGAT AAAACAGGAA TCTGGCAGCA GGAGTTCCTT GGAATCATTA 
GGAAAAAGTC AAGCAGATCG GAACAAAGTT ACTGCAGATA TGAAGATGTA CCACCTTGAC 
AACTGGCAGA GAACTGAATC TGGATATGAA AGTAGCGATC ACATCAGCAG CGGGTCAAGC 
AATCTGGATT CACCTATTAT TGAAGGGATT GGTTTTGCTG ATGGGAGGGG CTTGCAAGAA 
ACCAGCCTGT GCAGTGATCA GCAGCTGTCA AATGCACTGT TATCTACCTC ACCACAAACC 
AGAAGTAATC TGAATGGTGC AAACAATGAA CACCATGCTC TGGAGCACTG CAAATCTGAA 
GACAGGCTTT CTATTGTTAC CCGGGAACCT GCACACAGCC CGTCAGGTCT CAGGAAGCAA 
TTTGTTGAAT TGAGAAGAGA CCTACGCTCA CCAAACCTGT TGTCAGCATC AAACGATGAT 
GTGAAGGAAT CGGAGGATAG TCTGGAAAGT TCTAGCTGGT TGAATTCTTG TCCCTCGGAC 
AACTGTCGTT TGCACCATGA AAATATTCAC TCGTTACGAG AAATTTCAAC AGGCCCAGCA 
AAAATGCTGA ATAATGGATT GTGTTCCCAG CGACAGCATG CAGCTAAAAC CTCTTCACAG 
CTGGCCTCTC CGAGCAACAG GCTGCAGTAT ATGAATTCTC AGTTCTTCGT ATTCAATGAG 
GATCAGTCAT GCAGCAGAGA TGAGCGCTGC TCTGTAGATC CTCCTGAAGT ACCTCCCCCT 
CTACCCCCCA AAAAATATGT AAGAAATGCG GCACAAAGAA CTGAACAAAG CTTTGCATCC 
ACAATCAGCA GTAAACTTTC TGAAACATTA GCACGAGCTT ATCCTGGAAT CTCTGAGAGA 
TATTTATCTG GGGATTTCAT TAGATCTGAG CCTGATAGTG GCTATTATCC AAAGGAAGAA 
TCATTTCATG GTACCGAAAT TAATACTAAA CCCGTCAACC TGGCTGGTTC TTCCTCTTAT 
GGCTGTCAGT CAAAGATAAA CTCACACACT ATTGGTAGAG ATGTTGCCCC ACATATAAAT 
GATACTATTG CTCCCACTAC TTACTTCTCA GTTGATAACT GTATGACAGA CACATACAGG 
ATGAAATATC ACCACAGGCC CAGAATGAAT ATGGGAGACC GTTCTGGGTA CCAGAGAGAT 
CCTGGTCATC TGCAGCTAAC GGATAAATTA ATGGAACCAG CATACCATAG AACTTTAGAA 
AATGCTTCAC GTTCCAAACA AACTTTGGGA GGTATACCAT GTAATCGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934301.1
CDS	1105..4335
Translation	MAWVKLFRRQAGGNLGKVYQPGSVLSLAPTKGLFNEPGQNSCFLNSAVQVLWQLDIFRRSLRGLTGHVCLGDACIFCALKSIFAQFQTSREKVLPSDAMRHALAESFKDEHRFQLGLMDDAAECFENILQRIHFHIVPNSEGDMCTSKSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVDRMLERHERLKPEMFADLLQAANTTDDYRKCPSNCGQRIKIRRVLMNCPEIVTIGLVWDSDNSDLTEDVVRNLATQLYLPGLFFRVTDEQAKESRLYLVGMICYTSKHYCAFAYHTKSAKWFLFDDASVKEVGTKWKDVVTKCIRCHYQPLLLFYANPDGTAVTNEDALRQVQHWSHCKRELHNEETGTERSSSVPKKPDYSRDHEFGEHIYQNNGYKFHMEEPAFNRGVVHSSAGRGPVKLNRNEQQEKLRDVSRECAQKANMKNLSSSQRKVTEKTQKKEIIRPKETSGGMAYNSKPSTVVHLKSESSPSFNGFRKYPDAHFFSSQGKGPYKQERPTSQPRQSSSFNAGHATIPESKQVARVKNNFSAGYDTDSSQEYREKPNSRNKKTWKPMRETLNVDSIFNDTDKSQHSPRHLVGSNGKQSARDHSFNPPSKESPKRNTLMTIFEDEIKQESGSRSSLESLGKSQADRNKVTADMKMYHLDNWQRTESGYESSDHISSGSSNLDSPIIEGIGFADGRGLQETSLCSDQQLSNALLSTSPQTRSNLNGANNEHHALEHCKSEDRLSIVTREPAHSPSGLRKQFVELRRDLRSPNLLSASNDDVKESEDSLESSSWLNSCPSDNCRLHHENIHSLREISTGPAKMLNNGLCSQRQHAAKTSSQLASPSNRLQYMNSQFFVFNEDQSCSRDERCSVDPPEVPPPLPPKKYVRNAAQRTEQSFASTISSKLSETLARAYPGISERYLSGDFIRSEPDSGYYPKEESFHGTEINTKPVNLAGSSSYGCQSKINSHTIGRDVAPHINDTIAPTTYFSVDNCMTDTYRMKYHHRPRMNMGDRSGYQRDPGHLQLTDKLMEPAYHRTLENASRSKQTLGGIPCNR

Target ORF information:

RefSeq Version	XM_002934255.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 53 (usp53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934255.4

ORF Insert Sequence:

ATGGCCTGGG TGAAGCTTTT TCGGCGTCAG GCAGGAGGGA ACCTAGGGAA AGTGTACCAG 
CCTGGCAGCG TCCTCTCTCT GGCACCTACC AAGGGCCTGT TCAATGAGCC CGGGCAGAAC 
AGCTGCTTCC TGAACAGCGC CGTGCAGGTT CTGTGGCAAC TGGACATTTT TCGACGCAGT 
TTACGCGGAC TGACTGGACA TGTTTGCTTA GGAGATGCTT GTATATTTTG TGCATTAAAG 
TCCATATTTG CACAGTTTCA AACAAGCCGA GAGAAGGTTC TACCATCGGA TGCTATGAGA 
CATGCTCTTG CAGAAAGCTT CAAAGATGAG CATCGCTTTC AGCTTGGCCT TATGGATGAT 
GCTGCTGAAT GTTTTGAAAA CATATTGCAG CGGATTCATT TCCATATTGT GCCAAATAGT 
GAAGGAGATA TGTGCACTTC AAAATCTTGT ATCACCCACC AGAAGTTTGC AATGACTCTT 
TATGAACAGT GTGTTTGCCG CAGCTGTGGA GCTTCCTCTG ATCCATTGCC TTTTACTGAG 
TTTGTGAGAT ACATTTCTAC AACAGCACTT TGTAATGAAG TTGACCGAAT GCTAGAGAGG 
CATGAACGCT TGAAGCCAGA AATGTTTGCC GATTTACTTC AGGCTGCTAA CACCACAGAT 
GATTACAGGA AGTGTCCGAG TAACTGTGGC CAGAGAATCA AAATCCGGCG TGTACTTATG 
AACTGCCCAG AGATTGTTAC TATTGGCCTG GTTTGGGATT CTGATAACTC TGACTTAACT 
GAAGATGTTG TGCGAAACTT GGCTACTCAG CTTTATTTAC CCGGGCTGTT TTTCAGGGTT 
ACTGATGAAC AAGCCAAAGA AAGTAGACTT TACCTTGTCG GTATGATCTG CTACACGAGT 
AAACACTACT GTGCCTTTGC CTATCACACA AAGAGTGCCA AGTGGTTTTT GTTTGATGAT 
GCATCCGTAA AAGAGGTTGG TACAAAGTGG AAAGATGTGG TCACCAAGTG CATTCGGTGT 
CATTACCAGC CTTTGCTTCT GTTTTATGCA AATCCTGATG GCACGGCAGT AACTAATGAG 
GATGCACTAA GACAGGTTCA GCATTGGTCT CACTGTAAAA GAGAGTTACA TAATGAGGAG 
ACAGGTACTG AAAGATCATC CTCTGTACCA AAGAAGCCAG ATTACAGCAG AGATCATGAG 
TTTGGTGAAC ACATATATCA GAACAATGGG TACAAATTCC ATATGGAAGA ACCAGCTTTT 
AATAGAGGAG TTGTTCATTC GAGTGCTGGC AGAGGGCCTG TTAAACTAAA TCGTAATGAA 
CAACAAGAAA AACTGAGGGA TGTTTCTAGA GAATGTGCAC AGAAAGCAAA CATGAAAAAT 
TTATCATCCT CACAAAGAAA AGTTACTGAG AAAACACAGA AGAAAGAAAT CATCAGACCC 
AAGGAGACTT CAGGAGGAAT GGCATACAAT TCAAAGCCTA GCACAGTAGT GCATCTCAAG 
TCTGAATCCT CCCCCTCTTT TAATGGCTTT AGGAAGTATC CTGATGCTCA TTTCTTTAGT 
AGTCAAGGGA AAGGTCCGTA CAAGCAAGAA AGACCAACGA GCCAGCCTAG ACAATCTTCT 
TCTTTTAATG CAGGGCATGC CACAATTCCT GAATCCAAGC AAGTGGCCAG GGTGAAAAAC 
AATTTTTCAG CTGGTTATGA TACAGACAGT AGTCAGGAAT ACAGAGAAAA ACCAAACTCC 
AGAAATAAAA AAACTTGGAA GCCTATGCGG GAGACACTTA ATGTGGATAG CATTTTCAAT 
GACACTGATA AAAGCCAACA TAGCCCAAGA CATTTAGTAG GCTCAAATGG AAAACAATCT 
GCCAGGGATC ATAGTTTTAA CCCACCATCA AAAGAGAGCC CAAAACGTAA TACTCTGATG 
ACTATCTTTG AGGATGAGAT AAAACAGGAA TCTGGCAGCA GGAGTTCCTT GGAATCATTA 
GGAAAAAGTC AAGCAGATCG GAACAAAGTT ACTGCAGATA TGAAGATGTA CCACCTTGAC 
AACTGGCAGA GAACTGAATC TGGATATGAA AGTAGCGATC ACATCAGCAG CGGGTCAAGC 
AATCTGGATT CACCTATTAT TGAAGGGATT GGTTTTGCTG ATGGGAGGGG CTTGCAAGAA 
ACCAGCCTGT GCAGTGATCA GCAGCTGTCA AATGCACTGT TATCTACCTC ACCACAAACC 
AGAAGTAATC TGAATGGTGC AAACAATGAA CACCATGCTC TGGAGCACTG CAAATCTGAA 
GACAGGCTTT CTATTGTTAC CCGGGAACCT GCACACAGCC CGTCAGGTCT CAGGAAGCAA 
TTTGTTGAAT TGAGAAGAGA CCTACGCTCA CCAAACCTGT TGTCAGCATC AAACGATGAT 
GTGAAGGAAT CGGAGGATAG TCTGGAAAGT TCTAGCTGGT TGAATTCTTG TCCCTCGGAC 
AACTGTCGTT TGCACCATGA AAATATTCAC TCGTTACGAG AAATTTCAAC AGGCCCAGCA 
AAAATGCTGA ATAATGGATT GTGTTCCCAG CGACAGCATG CAGCTAAAAC CTCTTCACAG 
CTGGCCTCTC CGAGCAACAG GCTGCAGTAT ATGAATTCTC AGTTCTTCGT ATTCAATGAG 
GATCAGTCAT GCAGCAGAGA TGAGCGCTGC TCTGTAGATC CTCCTGAAGT ACCTCCCCCT 
CTACCCCCCA AAAAATATGT AAGAAATGCG GCACAAAGAA CTGAACAAAG CTTTGCATCC 
ACAATCAGCA GTAAACTTTC TGAAACATTA GCACGAGCTT ATCCTGGAAT CTCTGAGAGA 
TATTTATCTG GGGATTTCAT TAGATCTGAG CCTGATAGTG GCTATTATCC AAAGGAAGAA 
TCATTTCATG GTACCGAAAT TAATACTAAA CCCGTCAACC TGGCTGGTTC TTCCTCTTAT 
GGCTGTCAGT CAAAGATAAA CTCACACACT ATTGGTAGAG ATGTTGCCCC ACATATAAAT 
GATACTATTG CTCCCACTAC TTACTTCTCA GTTGATAACT GTATGACAGA CACATACAGG 
ATGAAATATC ACCACAGGCC CAGAATGAAT ATGGGAGACC GTTCTGGGTA CCAGAGAGAT 
CCTGGTCATC TGCAGCTAAC GGATAAATTA ATGGAACCAG CATACCATAG AACTTTAGAA 
AATGCTTCAC GTTCCAAACA AACTTTGGGA GGTATACCAT GTAATCGGTA G