mios cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	mios
Entrez Gene ID	100145742
Full Name	missing oocyte, meiosis regulator, homolog
General protein information	Preferred Names missing oocyte, meiosis regulator, homolog Names WD repeat-containing protein mio
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004915419.4	XP_004915476.2	GATOR complex protein MIOS
XM_004915419.3	XP_004915476.1	WD repeat-containing protein mio

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MIOS	XP_001144783.1
Drosophila melanogaster (fruit fly)	mio	NP_608656.1
Xenopus tropicalis (tropical clawed frog)	mios	XP_004915476.1
	MIOS	XP_001087456.1
Canis lupus familiaris (dog)	MIOS	XP_005628601.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007984	XP_317482.4
Homo sapiens (human)	MIOS	NP_061878.3
Bos taurus (cattle)	MIOS	NP_001179247.1
Gallus gallus (chicken)	MIOS	XP_418678.2
Rattus norvegicus (Norway rat)	Mios	NP_001167025.1
Mus musculus (house mouse)	Mios	NP_663349.2
Danio rerio (zebrafish)	mios	NP_958490.1

Gene Ontology

The following mios gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mios cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa11995	XM_004915419.3 Latest version!	Xenopus tropicalis missing oocyte, meiosis regulator, homolog (mios), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa60116	XM_004915419.4 Latest version!	Xenopus tropicalis missing oocyte, meiosis regulator, homolog (mios), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mios ( XM_004915419.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915476.1 Xenopus tropicalis missing oocyte, meiosis regulator, homolog (mios), mRNA.

Price & Availability↑

CloneID	OXa11995
Clone ID Related Accession (Same CDS sequence)	XM_004915419.3
Accession Version	XM_004915419.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	WD repeat-containing protein mio
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683410.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915419.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCT CCAAGCCTGA CATCCTCTGG GCACCTCATC ATGTGGACAG ATTTGTTGTA 
TGTGATTCTG AGCTCAACCT TTACCACATA GAATCTGCCG CAAGTCCCAG CTCTGAATTA 
AAAGCAGGAT CCCTGCGGCT GTCTGAAGAG ACCACAGCCA CTTTACTGGC TATAAATTCT 
GACACTCCTT ACATGAAATG TGTAGCCTGG TATCCCAAGT ATGACCCAGA ATGTCTTTTG 
GCTGTCGGTC AGGCCAATGG AAGAGTTGTG CTAACAAGTC TGGGCCAGGA CCACAATTCT 
AAATGTAAAG ACTTGATTGG AAAGGAGTTT GTCCCCAAAC ATGCACGTCA GTGTAACACG 
TTGGCTTGGA ACCCCCTTGA CAGTAATTGG CTTGCAGCTG GGTTAGACAA ACACCGGGCT 
GACTTCTCAG TATTGATCTG GGACATAAGC AGCAAGTATA CTCCAGAGGT AGCAGTTCCT 
ACGGAGAAAG TCCGTCTTGC ACCCGGTGAT AGTGAAACAT GTTCAGTGGT TACCAAACCA 
CTTTATGAAT TAGGACAGAA TGATGCTTGC CTCTCTCTTT GTTGGCTTCC GAGAGACCAG 
AAACTGCTTT TGGCTGGGAT GCATCGTAAC TTGGCAATAT TTGATTTGAG GAATACAAAT 
CAGAAAATTT TTGTAAATAC TAAAGCTGTC CAAGGGGTGA CAGTGGACCC GCATTTCCAT 
GACAGAGTAG CTTCATTTTT TGAAGGTCAA GTCGCCATTT GGGATCTTCG AAAATTTGAA 
AAACCAGTGT TAACGTTAAC AGAGCAACCA AAACCCCTAA CCAAAGTAGC CTGGTGCCCA 
ACTAGAACTG GACTGCTGGC TACTCTGACT AGAGACAGTA ATATTATCAG ACTGTATGAC 
ATGCAGCACA CTCCCACACC CATTGGGGAC GAAACAGAGC CTACCATCAT TGAAAGGAGT 
GTCCAGCCTT GTGATAACTA TATTGCATCT TTTGCTTGGC ACCCTACAAG CCAAAACCGA 
ATGGTGGTCG TTACTCCTAA CAGGTCAATG TCAGATTTTA CTGTGTTTGA GAGGATCTCT 
TTGGCTTGGA GCCCCACAAC TTCACTGATG TGGGCTTGCA GCAGGCAGCT GTATGAATGT 
ACAGAGGAAG GGAAATGCAG CTCTTCATTA GATCGAGATA TAGCCACTAA AATGAGACTC 
AGAGCTTTAT CACGATATGG ACTCGACACA GAGCAGGTCT GGAGAAATCA TCTGTTGGCA 
GGAAATGATG ATCCTCAGTT GAAATCCTTG TGGTACACTT TACACTTTAT GAAGCAGTAT 
GCAGAAGATA TTGACCAAAA AACTTCAGGA AACAAAGGGT TATTGGTGTA TGCTGGAATC 
AAAGCAATCA CAAAGTCATC AATGGGGACA ACTGAAAGTT TCAGGCACAG CTGGACCGGC 
TCTGATAGAC AAGCAGATAT TGTGCACTTT TCCAGTGAGG AAAGATGTCT TGCCCTACAA 
CTTTGTGGCT GGAGGACAAA GGGTGCTGAT GCCGACATGG GGCATTTTCT GAACTCCTTG 
GAGCAGGAAG GAGAGTGGGA GAGAGCAGCT GCTATAGCCC TGTTTAATAG AGACATTCGG 
CGTGCCATAC AGATCTTAAA TAAGGGAGCA TCCTCTGGGA AAGGTAATCT AAACCTAAAC 
GTTGTAGCAA TGGCACTGTC AGGCTATACT GACGAGAAGA ATTCCTTGTG GAGAGAAATG 
TGCAGCACTT TAAGGCTACA GCTTAACAAC CCATATCTTT GTGTTATGTT TGCTTTTCTC 
ACAAGTGAAC CTGGTGTATA TGACGGTGTT CTGTATGAGA ACAGCATGGC AGTGAGGGAT 
CGTGTGGCGT TTGCATGTTT GTTCCTTAAT GACGTTCAGT TAAGCAGATA CATTGACAAG 
CTGACTAATG AAATGAAGGA AGCTGGAAAC CTAGAAGGAA TTTTACTAAC TGGACTGACA 
AAAGATGGAG TTGACTTAAT AGAAAGTTAC GTTGATCGAA CTGGAGATGT TCAGACAGCA 
AGCTACTGTA TGCTGCAGGG ATCTCCATCT GACGTCCTTA AAGATGAAAG AGTTCAGTAC 
TGGATAGAGA GCTATAGGAA TCTGCTTGAT GCATGGAGAT TTTGGCACAA GAGAGCCGAG 
TTTGATATTC ATCGAAGTAA GCTTGACCCA AGCTCCAAGC CATTAGCACA GGTGTTTGTG 
AGCTGCAATT TTTGTGGAAA GTCCATCTCC TACAGTTGTT CATCTGTACC TCACCAAGGC 
CGTGGATTCA GTCAGTATGG TGTCAGTGGG TCGCCAACTA AGTCTAAATT CACCAGTTGT 
CCTGGATGTC GCAAACCTCT TCCCCGCTGT GCACTTTGTC TCATAAACAT GGGAAACCCT 
GTCTCTAGCA GCCCAGGAGC ATCAAAGTCT GATGAAAAGG TGGATCTCAG CAAGGAAAAG 
AAGGTGGCAC AGTTTAACAA CTGGTTCACG TGGTGTCACA ACTGCCGACA TGGTGGCCAT 
GCAGGACATA TGCTTAGCTG GTTTAAATGT CACAGCGAGT GCCCAGTATC TGCGTGCTCG 
TGTAAGTGTA TGCAGTTGGA CACAACTGGA AATCTTGTCC CAGCAGAGAA TGTTCAGGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915476.1
CDS	288..2930
Translation	MSGSKPDILWAPHHVDRFVVCDSELNLYHIESAASPSSELKAGSLRLSEETTATLLAINSDTPYMKCVAWYPKYDPECLLAVGQANGRVVLTSLGQDHNSKCKDLIGKEFVPKHARQCNTLAWNPLDSNWLAAGLDKHRADFSVLIWDISSKYTPEVAVPTEKVRLAPGDSETCSVVTKPLYELGQNDACLSLCWLPRDQKLLLAGMHRNLAIFDLRNTNQKIFVNTKAVQGVTVDPHFHDRVASFFEGQVAIWDLRKFEKPVLTLTEQPKPLTKVAWCPTRTGLLATLTRDSNIIRLYDMQHTPTPIGDETEPTIIERSVQPCDNYIASFAWHPTSQNRMVVVTPNRSMSDFTVFERISLAWSPTTSLMWACSRQLYECTEEGKCSSSLDRDIATKMRLRALSRYGLDTEQVWRNHLLAGNDDPQLKSLWYTLHFMKQYAEDIDQKTSGNKGLLVYAGIKAITKSSMGTTESFRHSWTGSDRQADIVHFSSEERCLALQLCGWRTKGADADMGHFLNSLEQEGEWERAAAIALFNRDIRRAIQILNKGASSGKGNLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLNNPYLCVMFAFLTSEPGVYDGVLYENSMAVRDRVAFACLFLNDVQLSRYIDKLTNEMKEAGNLEGILLTGLTKDGVDLIESYVDRTGDVQTASYCMLQGSPSDVLKDERVQYWIESYRNLLDAWRFWHKRAEFDIHRSKLDPSSKPLAQVFVSCNFCGKSISYSCSSVPHQGRGFSQYGVSGSPTKSKFTSCPGCRKPLPRCALCLINMGNPVSSSPGASKSDEKVDLSKEKKVAQFNNWFTWCHNCRHGGHAGHMLSWFKCHSECPVSACSCKCMQLDTTGNLVPAENVQA

Target ORF information:

RefSeq Version	XM_004915419.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis missing oocyte, meiosis regulator, homolog (mios), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915419.3

ORF Insert Sequence:

ATGAGTGGCT CCAAGCCTGA CATCCTCTGG GCACCTCATC ATGTGGACAG ATTTGTTGTA 
TGTGATTCTG AGCTCAACCT TTACCACATA GAATCTGCCG CAAGTCCCAG CTCTGAATTA 
AAAGCAGGAT CCCTGCGGCT GTCTGAAGAG ACCACAGCCA CTTTACTGGC TATAAATTCT 
GACACTCCTT ACATGAAATG TGTAGCCTGG TATCCCAAGT ATGACCCAGA ATGTCTTTTG 
GCTGTCGGTC AGGCCAATGG AAGAGTTGTG CTAACAAGTC TGGGCCAGGA CCACAATTCT 
AAATGTAAAG ACTTGATTGG AAAGGAGTTT GTCCCCAAAC ATGCACGTCA GTGTAACACG 
TTGGCTTGGA ACCCCCTTGA CAGTAATTGG CTTGCAGCTG GGTTAGACAA ACACCGGGCT 
GACTTCTCAG TATTGATCTG GGACATAAGC AGCAAGTATA CTCCAGAGGT AGCAGTTCCT 
ACGGAGAAAG TCCGTCTTGC ACCCGGTGAT AGTGAAACAT GTTCAGTGGT TACCAAACCA 
CTTTATGAAT TAGGACAGAA TGATGCTTGC CTCTCTCTTT GTTGGCTTCC GAGAGACCAG 
AAACTGCTTT TGGCTGGGAT GCATCGTAAC TTGGCAATAT TTGATTTGAG GAATACAAAT 
CAGAAAATTT TTGTAAATAC TAAAGCTGTC CAAGGGGTGA CAGTGGACCC GCATTTCCAT 
GACAGAGTAG CTTCATTTTT TGAAGGTCAA GTCGCCATTT GGGATCTTCG AAAATTTGAA 
AAACCAGTGT TAACGTTAAC AGAGCAACCA AAACCCCTAA CCAAAGTAGC CTGGTGCCCA 
ACTAGAACTG GACTGCTGGC TACTCTGACT AGAGACAGTA ATATTATCAG ACTGTATGAC 
ATGCAGCACA CTCCCACACC CATTGGGGAC GAAACAGAGC CTACCATCAT TGAAAGGAGT 
GTCCAGCCTT GTGATAACTA TATTGCATCT TTTGCTTGGC ACCCTACAAG CCAAAACCGA 
ATGGTGGTCG TTACTCCTAA CAGGTCAATG TCAGATTTTA CTGTGTTTGA GAGGATCTCT 
TTGGCTTGGA GCCCCACAAC TTCACTGATG TGGGCTTGCA GCAGGCAGCT GTATGAATGT 
ACAGAGGAAG GGAAATGCAG CTCTTCATTA GATCGAGATA TAGCCACTAA AATGAGACTC 
AGAGCTTTAT CACGATATGG ACTCGACACA GAGCAGGTCT GGAGAAATCA TCTGTTGGCA 
GGAAATGATG ATCCTCAGTT GAAATCCTTG TGGTACACTT TACACTTTAT GAAGCAGTAT 
GCAGAAGATA TTGACCAAAA AACTTCAGGA AACAAAGGGT TATTGGTGTA TGCTGGAATC 
AAAGCAATCA CAAAGTCATC AATGGGGACA ACTGAAAGTT TCAGGCACAG CTGGACCGGC 
TCTGATAGAC AAGCAGATAT TGTGCACTTT TCCAGTGAGG AAAGATGTCT TGCCCTACAA 
CTTTGTGGCT GGAGGACAAA GGGTGCTGAT GCCGACATGG GGCATTTTCT GAACTCCTTG 
GAGCAGGAAG GAGAGTGGGA GAGAGCAGCT GCTATAGCCC TGTTTAATAG AGACATTCGG 
CGTGCCATAC AGATCTTAAA TAAGGGAGCA TCCTCTGGGA AAGGTAATCT AAACCTAAAC 
GTTGTAGCAA TGGCACTGTC AGGCTATACT GACGAGAAGA ATTCCTTGTG GAGAGAAATG 
TGCAGCACTT TAAGGCTACA GCTTAACAAC CCATATCTTT GTGTTATGTT TGCTTTTCTC 
ACAAGTGAAC CTGGTGTATA TGACGGTGTT CTGTATGAGA ACAGCATGGC AGTGAGGGAT 
CGTGTGGCGT TTGCATGTTT GTTCCTTAAT GACGTTCAGT TAAGCAGATA CATTGACAAG 
CTGACTAATG AAATGAAGGA AGCTGGAAAC CTAGAAGGAA TTTTACTAAC TGGACTGACA 
AAAGATGGAG TTGACTTAAT AGAAAGTTAC GTTGATCGAA CTGGAGATGT TCAGACAGCA 
AGCTACTGTA TGCTGCAGGG ATCTCCATCT GACGTCCTTA AAGATGAAAG AGTTCAGTAC 
TGGATAGAGA GCTATAGGAA TCTGCTTGAT GCATGGAGAT TTTGGCACAA GAGAGCCGAG 
TTTGATATTC ATCGAAGTAA GCTTGACCCA AGCTCCAAGC CATTAGCACA GGTGTTTGTG 
AGCTGCAATT TTTGTGGAAA GTCCATCTCC TACAGTTGTT CATCTGTACC TCACCAAGGC 
CGTGGATTCA GTCAGTATGG TGTCAGTGGG TCGCCAACTA AGTCTAAATT CACCAGTTGT 
CCTGGATGTC GCAAACCTCT TCCCCGCTGT GCACTTTGTC TCATAAACAT GGGAAACCCT 
GTCTCTAGCA GCCCAGGAGC ATCAAAGTCT GATGAAAAGG TGGATCTCAG CAAGGAAAAG 
AAGGTGGCAC AGTTTAACAA CTGGTTCACG TGGTGTCACA ACTGCCGACA TGGTGGCCAT 
GCAGGACATA TGCTTAGCTG GTTTAAATGT CACAGCGAGT GCCCAGTATC TGCGTGCTCG 
TGTAAGTGTA TGCAGTTGGA CACAACTGGA AATCTTGTCC CAGCAGAGAA TGTTCAGGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mios ( XM_004915419.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915476.2 Xenopus tropicalis missing oocyte, meiosis regulator, homolog (mios), mRNA.

Price & Availability↑

CloneID	OXa60116
Clone ID Related Accession (Same CDS sequence)	XM_004915419.4
Accession Version	XM_004915419.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	GATOR complex protein MIOS
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915419.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCT CCAAGCCTGA CATCCTCTGG GCACCTCATC ATGTGGACAG ATTTGTTGTA 
TGTGATTCTG AGCTCAACCT TTACCACATA GAATCTGCCG CAAGTCCCAG CTCTGAATTA 
AAAGCAGGAT CCCTGCGGCT GTCTGAAGAG ACCACAGCCA CTTTACTGGC TATAAATTCT 
GACACTCCTT ACATGAAATG TGTAGCCTGG TATCCCAAGT ATGACCCAGA ATGTCTTTTG 
GCTGTCGGTC AGGCCAATGG AAGAGTTGTG CTAACAAGTC TGGGCCAGGA CCACAATTCT 
AAATGTAAAG ACTTGATTGG AAAGGAGTTT GTCCCCAAAC ATGCACGTCA GTGTAACACG 
TTGGCTTGGA ACCCCCTTGA CAGTAATTGG CTTGCAGCTG GGTTAGACAA ACACCGGGCT 
GACTTCTCAG TATTGATCTG GGACATAAGC AGCAAGTATA CTCCAGAGGT AGCAGTTCCT 
ACGGAGAAAG TCCGTCTTGC ACCCGGTGAT AGTGAAACAT GTTCAGTGGT TACCAAACCA 
CTTTATGAAT TAGGACAGAA TGATGCTTCC CTCTCTCTTT GTTGGCTTCC GAGAGACCAG 
AAACTGCTTT TGGCTGGGAT GCATCGTAAC TTGGCAATAT TTGATTTGAG GAATACAAAT 
CAGAAAATTT TTGTAAATAC TAAAGCTGTC CAAGGGGTGA CAGTGGACCC GCATTTCCAT 
GACAGAGTAG CTTCATTTTT TGAAGGTCAA GTCGCCATTT GGGATCTTCG AAAATTTGAA 
AAACCAGTGT TAACGTTAAC AGAGCAACCA AAACCCCTAA CCAAAGTAGC CTGGTGCCCA 
ACTAGAACTG GACTGCTGGC TACTCTGACT AGAGACAGTA ATATTATCAG ACTGTATGAC 
ATGCAGCACA CTCCCACACC CATTGGGGAC GAAACAGAGC CTACCATCAT TGAAAGGAGT 
GTCCAGCCTT GTGATAACTA TATTGCATCT TTTGCTTGGC ACCCTACAAG CCAAAACCGA 
ATGGTGGTCG TTACTCCTAA CAGGTCAATG TCAGATTTTA CTGTGTTTGA GAGGATCTCT 
TTGGCTTGGA GCCCCACAAC TTCACTGATG TGGGCTTGCA GCAGGCAGCT GTATGAATGT 
ACAGAGGAAG GGAAATGCAG CTCTTCATTA GATCGAGATA TAGCCACTAA AATGAGACTC 
AGAGCTTTAT CACGATATGG ACTCGACACA GAGCAGGTCT GGAGAAATCA TCTGTTGGCA 
GGAAATGATG ATCCTCAGTT GAAATCCTTG TGGTACACTT TACACTTTAT GAAGCAGTAT 
GCAGAAGATA TTGACCAAAA AACTTCAGGA AACAAAGGGT TATTGGTGTA TGCTGGAATC 
AAAGCAATCA CAAAGTCATC AATGGGGACA ACTGAAAGTT TCAGGCACAG CTGGACCGGC 
TCTGATAGAC AAGCAGATAT TGTGCACTTT TCCAGTGAGG AAAGATGTCT TGCCCTACAA 
CTTTGTGGCT GGAGGACAAA GGGTGCTGAT GCCGACATGG GGCATTTTCT GAACTCCTTG 
GAGCAGGAAG GAGAGTGGGA GAGAGCAGCT GCTATAGCCC TGTTTAATAG AGACATTCGG 
CGTGCCATAC AGATCTTAAA TAAGGGAGCA TCCACTGGGA AAGGTAATCT AAACCTAAAC 
GTTGTAGCAA TGGCACTGTC AGGCTATACT GACGAGAAGA ATTCCTTGTG GAGAGAAATG 
TGCAGCACTT TAAGGCTACA GCTTAACAAC CCATATCTTT GTGTTATGTT TGCTTTTCTC 
ACAAGTGAAC CTGGTGTATA TGACGGTGTT CTGTATGAGA ACAGCATGGC AGTGAGGGAT 
CGTGTGGCGT TTGCATGTTT GTTCCTTAAT GACGTTCAGT TAAGCAGATA CATTGACAAG 
CTAACTAATG AAATGAAGGA AGCTGGAAAC CTAGAAGGAA TTTTACTAAC TGGACTGACA 
AAAGATGGAG TTGACTTAAT AGAAAGTTAC GTTGATCGAA CTGGAGATGT TCAGACAGCA 
AGCTACTGTA TGCTGCAGGG ATCTCCATCT GACGTCCTTA AAGATGAAAG AGTTCAGTAC 
TGGATAGAGA GCTATAGGAA TCTGCTTGAT GCATGGAGAT TTTGGCACAA GAGAGCCGAG 
TTTGATATTC ATCGAAGTAA GCTTGACCCA AGCTCCAAGC CATTAGCACA GGTGTTTGTG 
AGCTGCAATT TTTGTGGAAA GTCCATCTCC TACAGTTGTT CATCTGTACC TCACCAAGGC 
CGTGGATTCA GTCAGTATGG TGTCAGTGGG TCGCCAACTA AGTCTAAATT CACCAGCTGT 
CCTGGATGTC GCAAACCTCT TCCCCGCTGT GCACTTTGTC TCATAAACAT GGGAAACCCT 
GTCTCTAGCA GCCCAGGAGC ATCAAAGTCT GATGAAAAGG TGGATCTCAG CAAGGAAAAG 
AAGGTGGCAC AGTTTAACAA CTGGTTCACG TGGTGTCACA ACTGCCGACA TGGTGGCCAT 
GCAGGACATA TGCTTAGCTG GTTTAAATGT CACAGCGAGT GCCCAGTATC TGCGTGCTCG 
TGTAAGTGTA TGCAGTTGGA CACAACTGGA AATCTTGTCC CAGCAGAGAA TGTTCAGGCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915476.2
CDS	285..2927
Translation	MSGSKPDILWAPHHVDRFVVCDSELNLYHIESAASPSSELKAGSLRLSEETTATLLAINSDTPYMKCVAWYPKYDPECLLAVGQANGRVVLTSLGQDHNSKCKDLIGKEFVPKHARQCNTLAWNPLDSNWLAAGLDKHRADFSVLIWDISSKYTPEVAVPTEKVRLAPGDSETCSVVTKPLYELGQNDASLSLCWLPRDQKLLLAGMHRNLAIFDLRNTNQKIFVNTKAVQGVTVDPHFHDRVASFFEGQVAIWDLRKFEKPVLTLTEQPKPLTKVAWCPTRTGLLATLTRDSNIIRLYDMQHTPTPIGDETEPTIIERSVQPCDNYIASFAWHPTSQNRMVVVTPNRSMSDFTVFERISLAWSPTTSLMWACSRQLYECTEEGKCSSSLDRDIATKMRLRALSRYGLDTEQVWRNHLLAGNDDPQLKSLWYTLHFMKQYAEDIDQKTSGNKGLLVYAGIKAITKSSMGTTESFRHSWTGSDRQADIVHFSSEERCLALQLCGWRTKGADADMGHFLNSLEQEGEWERAAAIALFNRDIRRAIQILNKGASTGKGNLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLNNPYLCVMFAFLTSEPGVYDGVLYENSMAVRDRVAFACLFLNDVQLSRYIDKLTNEMKEAGNLEGILLTGLTKDGVDLIESYVDRTGDVQTASYCMLQGSPSDVLKDERVQYWIESYRNLLDAWRFWHKRAEFDIHRSKLDPSSKPLAQVFVSCNFCGKSISYSCSSVPHQGRGFSQYGVSGSPTKSKFTSCPGCRKPLPRCALCLINMGNPVSSSPGASKSDEKVDLSKEKKVAQFNNWFTWCHNCRHGGHAGHMLSWFKCHSECPVSACSCKCMQLDTTGNLVPAENVQA

Target ORF information:

RefSeq Version	XM_004915419.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis missing oocyte, meiosis regulator, homolog (mios), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915419.4

ORF Insert Sequence:

ATGAGTGGCT CCAAGCCTGA CATCCTCTGG GCACCTCATC ATGTGGACAG ATTTGTTGTA 
TGTGATTCTG AGCTCAACCT TTACCACATA GAATCTGCCG CAAGTCCCAG CTCTGAATTA 
AAAGCAGGAT CCCTGCGGCT GTCTGAAGAG ACCACAGCCA CTTTACTGGC TATAAATTCT 
GACACTCCTT ACATGAAATG TGTAGCCTGG TATCCCAAGT ATGACCCAGA ATGTCTTTTG 
GCTGTCGGTC AGGCCAATGG AAGAGTTGTG CTAACAAGTC TGGGCCAGGA CCACAATTCT 
AAATGTAAAG ACTTGATTGG AAAGGAGTTT GTCCCCAAAC ATGCACGTCA GTGTAACACG 
TTGGCTTGGA ACCCCCTTGA CAGTAATTGG CTTGCAGCTG GGTTAGACAA ACACCGGGCT 
GACTTCTCAG TATTGATCTG GGACATAAGC AGCAAGTATA CTCCAGAGGT AGCAGTTCCT 
ACGGAGAAAG TCCGTCTTGC ACCCGGTGAT AGTGAAACAT GTTCAGTGGT TACCAAACCA 
CTTTATGAAT TAGGACAGAA TGATGCTTCC CTCTCTCTTT GTTGGCTTCC GAGAGACCAG 
AAACTGCTTT TGGCTGGGAT GCATCGTAAC TTGGCAATAT TTGATTTGAG GAATACAAAT 
CAGAAAATTT TTGTAAATAC TAAAGCTGTC CAAGGGGTGA CAGTGGACCC GCATTTCCAT 
GACAGAGTAG CTTCATTTTT TGAAGGTCAA GTCGCCATTT GGGATCTTCG AAAATTTGAA 
AAACCAGTGT TAACGTTAAC AGAGCAACCA AAACCCCTAA CCAAAGTAGC CTGGTGCCCA 
ACTAGAACTG GACTGCTGGC TACTCTGACT AGAGACAGTA ATATTATCAG ACTGTATGAC 
ATGCAGCACA CTCCCACACC CATTGGGGAC GAAACAGAGC CTACCATCAT TGAAAGGAGT 
GTCCAGCCTT GTGATAACTA TATTGCATCT TTTGCTTGGC ACCCTACAAG CCAAAACCGA 
ATGGTGGTCG TTACTCCTAA CAGGTCAATG TCAGATTTTA CTGTGTTTGA GAGGATCTCT 
TTGGCTTGGA GCCCCACAAC TTCACTGATG TGGGCTTGCA GCAGGCAGCT GTATGAATGT 
ACAGAGGAAG GGAAATGCAG CTCTTCATTA GATCGAGATA TAGCCACTAA AATGAGACTC 
AGAGCTTTAT CACGATATGG ACTCGACACA GAGCAGGTCT GGAGAAATCA TCTGTTGGCA 
GGAAATGATG ATCCTCAGTT GAAATCCTTG TGGTACACTT TACACTTTAT GAAGCAGTAT 
GCAGAAGATA TTGACCAAAA AACTTCAGGA AACAAAGGGT TATTGGTGTA TGCTGGAATC 
AAAGCAATCA CAAAGTCATC AATGGGGACA ACTGAAAGTT TCAGGCACAG CTGGACCGGC 
TCTGATAGAC AAGCAGATAT TGTGCACTTT TCCAGTGAGG AAAGATGTCT TGCCCTACAA 
CTTTGTGGCT GGAGGACAAA GGGTGCTGAT GCCGACATGG GGCATTTTCT GAACTCCTTG 
GAGCAGGAAG GAGAGTGGGA GAGAGCAGCT GCTATAGCCC TGTTTAATAG AGACATTCGG 
CGTGCCATAC AGATCTTAAA TAAGGGAGCA TCCACTGGGA AAGGTAATCT AAACCTAAAC 
GTTGTAGCAA TGGCACTGTC AGGCTATACT GACGAGAAGA ATTCCTTGTG GAGAGAAATG 
TGCAGCACTT TAAGGCTACA GCTTAACAAC CCATATCTTT GTGTTATGTT TGCTTTTCTC 
ACAAGTGAAC CTGGTGTATA TGACGGTGTT CTGTATGAGA ACAGCATGGC AGTGAGGGAT 
CGTGTGGCGT TTGCATGTTT GTTCCTTAAT GACGTTCAGT TAAGCAGATA CATTGACAAG 
CTAACTAATG AAATGAAGGA AGCTGGAAAC CTAGAAGGAA TTTTACTAAC TGGACTGACA 
AAAGATGGAG TTGACTTAAT AGAAAGTTAC GTTGATCGAA CTGGAGATGT TCAGACAGCA 
AGCTACTGTA TGCTGCAGGG ATCTCCATCT GACGTCCTTA AAGATGAAAG AGTTCAGTAC 
TGGATAGAGA GCTATAGGAA TCTGCTTGAT GCATGGAGAT TTTGGCACAA GAGAGCCGAG 
TTTGATATTC ATCGAAGTAA GCTTGACCCA AGCTCCAAGC CATTAGCACA GGTGTTTGTG 
AGCTGCAATT TTTGTGGAAA GTCCATCTCC TACAGTTGTT CATCTGTACC TCACCAAGGC 
CGTGGATTCA GTCAGTATGG TGTCAGTGGG TCGCCAACTA AGTCTAAATT CACCAGCTGT 
CCTGGATGTC GCAAACCTCT TCCCCGCTGT GCACTTTGTC TCATAAACAT GGGAAACCCT 
GTCTCTAGCA GCCCAGGAGC ATCAAAGTCT GATGAAAAGG TGGATCTCAG CAAGGAAAAG 
AAGGTGGCAC AGTTTAACAA CTGGTTCACG TGGTGTCACA ACTGCCGACA TGGTGGCCAT 
GCAGGACATA TGCTTAGCTG GTTTAAATGT CACAGCGAGT GCCCAGTATC TGCGTGCTCG 
TGTAAGTGTA TGCAGTTGGA CACAACTGGA AATCTTGTCC CAGCAGAGAA TGTTCAGGCG 
TAA