dand5.S cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dand5.S
Entrez Gene ID	594864
Full Name	DAN domain family member 5, BMP antagonist S homeolog
Synonyms	51-B6,S10-51-B6,cer2,cerl2,cktsf1b3,coco,crl2,dand5,dante,grem3,xCoco
General protein information	Preferred Names DAN domain family member 5, BMP antagonist S homeolog Names DAN domain family member 5 DAN domain family, member 5 cerberus-like 2 protein cerl-2
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001098726.1	NP_001092196.1	DAN domain family member 5 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Coco is a dual activity modulator of TGF? signaling.
Deglincerti A, Haremaki T, Warmflash A, Sorre B, Brivanlou AH
Development (Cambridge, England)142(15)2678-85(2015 Aug)

Coco regulates dorsoventral specification of germ layers via inhibition of TGF? signalling.
Bates TJ, Vonica A, Heasman J, Brivanlou AH, Bell E
Development (Cambridge, England)140(20)4177-81(2013 Oct)

The nodal inhibitor Coco is a critical target of leftward flow in Xenopus.
Schweickert A, Vick P, Getwan M, Weber T, Schneider I, Eberhardt M, Beyer T, Pachur A, Blum M
Current biology : CB20(8)738-43(2010 Apr)

The left-right axis is regulated by the interplay of Coco, Xnr1 and derri?re in Xenopus embryos.
Vonica A, Brivanlou AH
Developmental biology303(1)281-94(2007 Mar)

The activity of the Nodal antagonist Cerl-2 in the mouse node is required for correct L/R body axis.
Marques S, Borges AC, Silva AC, Freitas S, Cordenonsi M, Belo JA
Genes & development18(19)2342-7(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Coco binds to TGFbeta1 and enhances TGFbeta1 binding to its receptor Alk5 thus acting as both an inhibitor and an enhancer of signaling, depending on the ligand it binds.
Title: Coco is a dual activity modulator of TGF? signaling.

Coco required to prevent Activin and Nodal signals in dorsal marginal side of embryo from invading the prospective ectoderm, thereby restricting endoderm- and mesoderm-inducing signals to the vegetal and marginal zones of the pre-gastrula Xenopus embryo
Title: Coco regulates dorsoventral specification of germ layers via inhibition of TGF? signalling.

Data suggest that Coco acts as a critical target of flow, suggesting that symmetry is broken by flow-mediated left-asymmetric release of Nodal repression at the midline.
Title: The nodal inhibitor Coco is a critical target of leftward flow in Xenopus.

Coco directly inhibits derriere and Xnr1, and with them define a posttranscriptionally regulated signaling center which is a necessary link in the signaling chain leading to an increased TGF-beta signal on the left side of the embryo.
Title: The left-right axis is regulated by the interplay of Coco, Xnr1 and derri?re in Xenopus embryos.

The following dand5.S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dand5.S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf03197	NM_001098726.1 Latest version!	Xenopus laevis DAN domain family member 5, BMP antagonist S homeolog (dand5.S), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dand5.S ( NM_001098726.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001092196.1 Xenopus laevis DAN domain family member 5, BMP antagonist S homeolog (dand5.S), mRNA.

Price & Availability↑

CloneID	OAf03197
Clone ID Related Accession (Same CDS sequence)	NM_001098726.1
Accession Version	NM_001098726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Xenopus laevis(African clawed frog)
Product	DAN domain family member 5 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC099052.1. ##Evidence-Data-START## Transcript exon combination :: BC099052.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGTTGT TCCAGGCCAC CAGCCTATTG GCCCTTCTCT GTTTCACGGT CAGGGCATTT 
CCCTTTATGG AAGAAGAAGG GTCGGCTTCG TTTGCCCAGA ATGTGCTCCA CAGCAGATCC 
TTCCCAGTCT CCCACCATGG AGCTTTCATG GACCTGCCGC TATTCAGACA GAACAGGAGG 
AAAATATCCC AGAATTTCAT CCTGCACTCA GATCCCAGAG AGCACATGGA TGAGGAGGCG 
CTGAGGAGAA AACTGGTGTG GGAGAGCGCC ATCCGCAGGG ACAAGATGAG ATCCCAACCA 
GACCAGGTGC TGCCCATTGG GCAAGATGCT CTGAAACGCT CCAGATGCCA TGCCTTGCCT 
TTCATACAGA ATGTGTTCAG GAAGAACTGC TTCCCAGTGC GCCTCCCTAA CAAGTTCTGC 
TTTGGCCAGT GCAACTCCTT CTATGTGCCT GGTTGGCCTG CGGGACTCTC CCAGCCCTGT 
ACGTCCTGCG CCCCCAGTCG ATCCCGGCGC ATTTCTCTGC CGTTACGCTG TCGTTCCGGT 
CACCTTGCTT GGCAAGAGGT GGAGCTGGTG GAAGAGTGCG AGTGCGAAAC CCGCTACGAC 
AGGAATACGG TGGAGCCAGC TGGCAGCGGA GAGGACTACC TGCCCGTTTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092196.1
CDS	99..752
Translation	MLLFQATSLLALLCFTVRAFPFMEEEGSASFAQNVLHSRSFPVSHHGAFMDLPLFRQNRRKISQNFILHSDPREHMDEEALRRKLVWESAIRRDKMRSQPDQVLPIGQDALKRSRCHALPFIQNVFRKNCFPVRLPNKFCFGQCNSFYVPGWPAGLSQPCTSCAPSRSRRISLPLRCRSGHLAWQEVELVEECECETRYDRNTVEPAGSGEDYLPVS

Target ORF information:

RefSeq Version	NM_001098726.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis DAN domain family member 5, BMP antagonist S homeolog (dand5.S), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098726.1

ORF Insert Sequence:

ATGCTGTTGT TCCAGGCCAC CAGCCTATTG GCCCTTCTCT GTTTCACGGT CAGGGCATTT 
CCCTTTATGG AAGAAGAAGG GTCGGCTTCG TTTGCCCAGA ATGTGCTCCA CAGCAGATCC 
TTCCCAGTCT CCCACCATGG AGCTTTCATG GACCTGCCGC TATTCAGACA GAACAGGAGG 
AAAATATCCC AGAATTTCAT CCTGCACTCA GATCCCAGAG AGCACATGGA TGAGGAGGCG 
CTGAGGAGAA AACTGGTGTG GGAGAGCGCC ATCCGCAGGG ACAAGATGAG ATCCCAACCA 
GACCAGGTGC TGCCCATTGG GCAAGATGCT CTGAAACGCT CCAGATGCCA TGCCTTGCCT 
TTCATACAGA ATGTGTTCAG GAAGAACTGC TTCCCAGTGC GCCTCCCTAA CAAGTTCTGC 
TTTGGCCAGT GCAACTCCTT CTATGTGCCT GGTTGGCCTG CGGGACTCTC CCAGCCCTGT 
ACGTCCTGCG CCCCCAGTCG ATCCCGGCGC ATTTCTCTGC CGTTACGCTG TCGTTCCGGT 
CACCTTGCTT GGCAAGAGGT GGAGCTGGTG GAAGAGTGCG AGTGCGAAAC CCGCTACGAC 
AGGAATACGG TGGAGCCAGC TGGCAGCGGA GAGGACTACC TGCCCGTTTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Coco is a dual activity modulator of TGF? signaling.
Development (Cambridge, England)142(15)2678-85(2015 Aug)
Deglincerti A,Haremaki T,Warmflash A,Sorre B,Brivanlou AH

Coco regulates dorsoventral specification of germ layers via inhibition of TGF? signalling.
Development (Cambridge, England)140(20)4177-81(2013 Oct)
Bates TJ,Vonica A,Heasman J,Brivanlou AH,Bell E

The nodal inhibitor Coco is a critical target of leftward flow in Xenopus.
Current biology : CB20(8)738-43(2010 Apr)
Schweickert A,Vick P,Getwan M,Weber T,Schneider I,Eberhardt M,Beyer T,Pachur A,Blum M

The left-right axis is regulated by the interplay of Coco, Xnr1 and derri?re in Xenopus embryos.
Developmental biology303(1)281-94(2007 Mar)
Vonica A,Brivanlou AH

The activity of the Nodal antagonist Cerl-2 in the mouse node is required for correct L/R body axis.
Genes & development18(19)2342-7(2004 Oct)
Marques S,Borges AC,Silva AC,Freitas S,Cordenonsi M,Belo JA