42sp50.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	42sp50.L
Entrez Gene ID	397889
Full Name	Elongation factor 1-alpha L homeolog
Synonyms	42sp50,Thesaurin-A
General protein information	Preferred Names Elongation factor 1-alpha L homeolog Names elongation factor 1-alpha EF-1-alpha thesaurin A
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001101761.1	NP_001095231.1	elongation factor 1-alpha
NM_001101761.2	NP_001095231.2	elongation factor 1-alpha

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes.
Loeber J, Claussen M, Jahn O, Pieler T
The FEBS journal277(22)4722-31(2010 Nov)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

Correction of the nucleotide and amino acid sequence of Xenopus laevis 42Sp50.
Cheng FM, Darby MK, Joho KE
Nucleic acids research21(9)2259(1993 May)

Structural and functional properties of thesaurin a (42Sp50), the major protein of the 42 S particles present in Xenopus laevis previtellogenic oocytes.
Viel A, le Maire M, Philippe H, Morales J, Mazabraud A, Denis H
The Journal of biological chemistry266(16)10392-9(1991 Jun)

Three genes under different developmental control encode elongation factor 1-alpha in Xenopus laevis.
Dj? MK, Mazabraud A, Viel A, le Maire M, Denis H, Crawford E, Brown DD
Nucleic acids research18(12)3489-93(1990 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data provide evidence for a novel function of 42Sp50 in the context of vegetal mRNA transport in Xenopus oocytes.
Title: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes.

The following 42sp50.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the 42sp50.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf00880	NM_001101761.1 Latest version!	Xenopus laevis Elongation factor 1-alpha L homeolog (42sp50.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OAf49233	NM_001101761.2 Latest version!	Xenopus laevis Elongation factor 1-alpha L homeolog (42sp50.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

42sp50.L ( NM_001101761.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001095231.1 Xenopus laevis Elongation factor 1-alpha L homeolog (42sp50.L), mRNA.

Price & Availability↑

CloneID	OAf00880
Clone ID Related Accession (Same CDS sequence)	NM_001101761.1
Accession Version	NM_001101761.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-28
Organism	Xenopus laevis(African clawed frog)
Product	elongation factor 1-alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X52975.1. On Aug 21, 2007 this sequence version replaced NM_001101762.1. ##Evidence-Data-START## Transcript exon combination :: X52975.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02777034, SAMN02851412 [ECO:0000348] ##Evidence-Data-END##

ATGACTGACA AGGCTCCTCA AAAGACTCAT TTGAACATTG TGATTATTGG ACATGTTGAT 
TCTGGGAAAT CCACCACCAC CGGACACCTC ATCTACAAGT GCGGGGGCTT TGACCCCAGG 
GCCCTGGAGA AGGTGGAGGC GGCTGCTGCT CAGCTTGGCA AGAGCTCCTT CAAGTTTGCC 
TGGATCTTGG ATAAGCTGAA GGCTGAGAGG GAGCGAGGAA TCACCATCGA CATCTCCCTA 
TGGAAGTTCC AGACCAACAG GTTCACAATC ACCATAATCG ATGCCCCGGG GCACAGGGAC 
TTCATTAAGA ACATGATCAC GGGCACCTCT CAGGCAGATG TTGCTCTCCT GGTGGTCTCT 
GCGGCTACAG GGGAATTTGA GGCCGGTGTG TCCAGAAATG GCCAAACAAG GGAACACGCT 
CTTCTGGCCT ACACCATGGG GGTCAAGCAA CTGATCGTCT GCGTGAACAA AATGGATCTG 
ACGGACCCTC CCTACAGCCA CAAGCGGTTT GATGAAGTTG TCAGGAATGT GATGGTCTAT 
CTGAAAAAGA TTGGGTACAA CCCGGCTACC ATCCCCTTCG TGCCTGTGTC TGGCTGGACG 
GGAGAGAATA TATCTTCGCC CAGTCAAAAG ATGGGTTGGT TTAAAGGTTG GAAGGTGAAA 
CGAAAAGATG GCTTTACAAA GGGCCAATCC CTCTTGGAGG TTCTGGATGC GCTTGTACCT 
CCAGTGAGGC CGGCAAATAA GCCTTTGCGG CTCCCCCCTG CATATGTATA CAAGATAGGG 
GGCATTGGTA CAGTCCCTGT GGGCAAGATA GAAACTGGGA TTCTGAAGCC AGGCATGACC 
ATCTCCTTTG CACCGTCTGG TTTCTCAGCT GAAGTTAAAT CCATAGAAAT GCACCACGAG 
CCGCTTCAGA TGGCCTTCCC AGGGTTCAAC ATCGGATTCA ATGTCAAGAA CATTGCTGCG 
AAAAGTCTAA AGCGTGGCAA TGTGGCGGGC AATTCAAAGA GTGACCCACC GACTGAGGCC 
TCCAGCTTCA CTGCCCAGGT GATCATTCTG AACCACCCGG GCTTTATCAA AGCCGGATAT 
TCACCGGTTA TCGACTGTCA CACTGCACAC ATCACATGCC AGTTTGCAGA ACTGCAGGAA 
AAGATTGACA GGCGGACTGG CAAAAAGCTA GAGGACAACC CGGGGCTACT GAAATCTGGA 
GATGCCGCCA TCATAACCCT GAAGCCCATC AAGCCCTTCT GTGTGGAGAG GTTCTTTGAT 
TATCCACCTC TAGGGAGGTT TGCAGCCCGA GACCTAAAAC AGACTGTTGC CGTCGGGGTT 
GTGAAGTCGG TGGAGCACAA AGCTGGAGCT GCTGCCAGGA GACAAGTCCA AAAACCAGTG 
TTGGTGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095231.1
CDS	294..1685
Translation	MTDKAPQKTHLNIVIIGHVDSGKSTTTGHLIYKCGGFDPRALEKVEAAAAQLGKSSFKFAWILDKLKAERERGITIDISLWKFQTNRFTITIIDAPGHRDFIKNMITGTSQADVALLVVSAATGEFEAGVSRNGQTREHALLAYTMGVKQLIVCVNKMDLTDPPYSHKRFDEVVRNVMVYLKKIGYNPATIPFVPVSGWTGENISSPSQKMGWFKGWKVKRKDGFTKGQSLLEVLDALVPPVRPANKPLRLPPAYVYKIGGIGTVPVGKIETGILKPGMTISFAPSGFSAEVKSIEMHHEPLQMAFPGFNIGFNVKNIAAKSLKRGNVAGNSKSDPPTEASSFTAQVIILNHPGFIKAGYSPVIDCHTAHITCQFAELQEKIDRRTGKKLEDNPGLLKSGDAAIITLKPIKPFCVERFFDYPPLGRFAARDLKQTVAVGVVKSVEHKAGAAARRQVQKPVLVK

Target ORF information:

RefSeq Version	NM_001101761.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis Elongation factor 1-alpha L homeolog (42sp50.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101761.1

ORF Insert Sequence:

ATGACTGACA AGGCTCCTCA AAAGACTCAT TTGAACATTG TGATTATTGG ACATGTTGAT 
TCTGGGAAAT CCACCACCAC CGGACACCTC ATCTACAAGT GCGGGGGCTT TGACCCCAGG 
GCCCTGGAGA AGGTGGAGGC GGCTGCTGCT CAGCTTGGCA AGAGCTCCTT CAAGTTTGCC 
TGGATCTTGG ATAAGCTGAA GGCTGAGAGG GAGCGAGGAA TCACCATCGA CATCTCCCTA 
TGGAAGTTCC AGACCAACAG GTTCACAATC ACCATAATCG ATGCCCCGGG GCACAGGGAC 
TTCATTAAGA ACATGATCAC GGGCACCTCT CAGGCAGATG TTGCTCTCCT GGTGGTCTCT 
GCGGCTACAG GGGAATTTGA GGCCGGTGTG TCCAGAAATG GCCAAACAAG GGAACACGCT 
CTTCTGGCCT ACACCATGGG GGTCAAGCAA CTGATCGTCT GCGTGAACAA AATGGATCTG 
ACGGACCCTC CCTACAGCCA CAAGCGGTTT GATGAAGTTG TCAGGAATGT GATGGTCTAT 
CTGAAAAAGA TTGGGTACAA CCCGGCTACC ATCCCCTTCG TGCCTGTGTC TGGCTGGACG 
GGAGAGAATA TATCTTCGCC CAGTCAAAAG ATGGGTTGGT TTAAAGGTTG GAAGGTGAAA 
CGAAAAGATG GCTTTACAAA GGGCCAATCC CTCTTGGAGG TTCTGGATGC GCTTGTACCT 
CCAGTGAGGC CGGCAAATAA GCCTTTGCGG CTCCCCCCTG CATATGTATA CAAGATAGGG 
GGCATTGGTA CAGTCCCTGT GGGCAAGATA GAAACTGGGA TTCTGAAGCC AGGCATGACC 
ATCTCCTTTG CACCGTCTGG TTTCTCAGCT GAAGTTAAAT CCATAGAAAT GCACCACGAG 
CCGCTTCAGA TGGCCTTCCC AGGGTTCAAC ATCGGATTCA ATGTCAAGAA CATTGCTGCG 
AAAAGTCTAA AGCGTGGCAA TGTGGCGGGC AATTCAAAGA GTGACCCACC GACTGAGGCC 
TCCAGCTTCA CTGCCCAGGT GATCATTCTG AACCACCCGG GCTTTATCAA AGCCGGATAT 
TCACCGGTTA TCGACTGTCA CACTGCACAC ATCACATGCC AGTTTGCAGA ACTGCAGGAA 
AAGATTGACA GGCGGACTGG CAAAAAGCTA GAGGACAACC CGGGGCTACT GAAATCTGGA 
GATGCCGCCA TCATAACCCT GAAGCCCATC AAGCCCTTCT GTGTGGAGAG GTTCTTTGAT 
TATCCACCTC TAGGGAGGTT TGCAGCCCGA GACCTAAAAC AGACTGTTGC CGTCGGGGTT 
GTGAAGTCGG TGGAGCACAA AGCTGGAGCT GCTGCCAGGA GACAAGTCCA AAAACCAGTG 
TTGGTGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

42sp50.L ( NM_001101761.2 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001095231.2 Xenopus laevis Elongation factor 1-alpha L homeolog (42sp50.L), mRNA.

Price & Availability↑

CloneID	OAf49233
Clone ID Related Accession (Same CDS sequence)	NM_001101761.2
Accession Version	NM_001101761.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Xenopus laevis(African clawed frog)
Product	elongation factor 1-alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC133768.1. On Jan 10, 2019 this sequence version replaced NM_001101761.1. ##Evidence-Data-START## Transcript exon combination :: BC133768.1, Z19545.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02777034, SAMN02851412 [ECO:0000348] ##Evidence-Data-END##

ATGACTGGCA AAGCTCCTCA GAAGACTCAC TTGAACATTG TGATTATTGG ACATGTTGAT 
TCTGGGAAAT CCACCACCAC CGGACACCTC ATCTACAAGT GCGGGGGCTT TGACCCCAGG 
GCCCTGGAGA AGGTGGAGGC GGCTGCTGCT CAGCTTGGCA AGAGCTCCTT CAAGTTTGCC 
TGGATCCTGG ATAAGCTGAA GGCTGAGAGG GAGCGAGGAA TCACCATCGA CATCTCCCTA 
TGGAAGTTCC AGACCAACAG GTTCACAATC ACCATAATCG ATGCCCCGGG GCACAGGGAC 
TTCATTAAGA ACATGATCAC AGGCACCTCT CAGGCAGATG TTGCTCTCCT GGTGGTCTCT 
GCGGCTACAG GGGAATTTGA GGCCGGTGTG TCCAGAAATG GCCAAACAAG GGAACACGCT 
CTTCTGGCCT ACACCATGGG GGTCAAGCAA CTGATCGTCT GCGTGAACAA AATGGATCTG 
ACGGACCCTC CCTACAGCCA CAAGAGATTT GATGAAGTTG TCAGGAATGT GATGGTCTAT 
CTGAAAAAGA TTGGGTACAA CCCGGCTACC ATCCCCTTCG TGCCCGTGTC TGGCTGGACG 
GGAGAGAATA TATCTTCGCC CAGTCAAAAG ATGGGTTGGT TTAAAGGTTG GAAGGTGAAA 
CGAAAAGATG GCTTTACAAA GGGCCAATCC CTCTTGGAGG TTCTGGATGC GCTTGTACCT 
CCAGTGAGGC CGGCAAATAA GCCTTTGCGG CTCCCCCTGC AGGATGTATA CAAGATAGGG 
GGCATTGGTA CAGTCCCTGT GGGCAAGATA GAAACTGGGA TTCTGAAGCC AGGCATGACC 
ATCTCCTTTG CACCGTCTGG TTTCTCAGCT GAAGTTAAAT CCATAGAAAT GCACCACGAG 
CCGCTTCAGA TGGCCTTCCC AGGGTTCAAC ATCGGATTCA ATGTCAAGAA CATTGCTGTG 
AAAAGTCTAA AGCGTGGCAA TGTGGCGGGC AATTCAAAGA GTGACCCACC GACTGAGGCC 
TCCAGCTTCA CTGCCCAGGT GATCATTCTG AACCACCCGG GCTTTATCAA AGCCGGATAT 
TCACCGGTTA TCGACTGTCA CACTGCACAC ATCACATGCC AGTTTGCAGA ACTGCAGGAA 
AAGATTGACA GGCGGACTGG CAAAAAGCTA GAGGACAACC CGGGGCTACT GAAATCTGGA 
GATGCCGCCA TCATAACCCT GAAGCCCATC AAGCCCTTCT GTGTGGAGAG GTTCTTTGAT 
TATCCACCTC TAGGGAGGTT TGCAGCCCGA GACCTAAAAC AGACTGTTGC CGTCGGGGTT 
GTGAAGTCGG TGGAGCACAA AGCTGGAGCT GCTGCCAGGA GACAAGTCCA AAAACCAGTG 
TTGGTGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095231.2
CDS	43..1434
Translation	MTGKAPQKTHLNIVIIGHVDSGKSTTTGHLIYKCGGFDPRALEKVEAAAAQLGKSSFKFAWILDKLKAERERGITIDISLWKFQTNRFTITIIDAPGHRDFIKNMITGTSQADVALLVVSAATGEFEAGVSRNGQTREHALLAYTMGVKQLIVCVNKMDLTDPPYSHKRFDEVVRNVMVYLKKIGYNPATIPFVPVSGWTGENISSPSQKMGWFKGWKVKRKDGFTKGQSLLEVLDALVPPVRPANKPLRLPLQDVYKIGGIGTVPVGKIETGILKPGMTISFAPSGFSAEVKSIEMHHEPLQMAFPGFNIGFNVKNIAVKSLKRGNVAGNSKSDPPTEASSFTAQVIILNHPGFIKAGYSPVIDCHTAHITCQFAELQEKIDRRTGKKLEDNPGLLKSGDAAIITLKPIKPFCVERFFDYPPLGRFAARDLKQTVAVGVVKSVEHKAGAAARRQVQKPVLVK

Target ORF information:

RefSeq Version	NM_001101761.2
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis Elongation factor 1-alpha L homeolog (42sp50.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101761.2

ORF Insert Sequence:

ATGACTGGCA AAGCTCCTCA GAAGACTCAC TTGAACATTG TGATTATTGG ACATGTTGAT 
TCTGGGAAAT CCACCACCAC CGGACACCTC ATCTACAAGT GCGGGGGCTT TGACCCCAGG 
GCCCTGGAGA AGGTGGAGGC GGCTGCTGCT CAGCTTGGCA AGAGCTCCTT CAAGTTTGCC 
TGGATCCTGG ATAAGCTGAA GGCTGAGAGG GAGCGAGGAA TCACCATCGA CATCTCCCTA 
TGGAAGTTCC AGACCAACAG GTTCACAATC ACCATAATCG ATGCCCCGGG GCACAGGGAC 
TTCATTAAGA ACATGATCAC AGGCACCTCT CAGGCAGATG TTGCTCTCCT GGTGGTCTCT 
GCGGCTACAG GGGAATTTGA GGCCGGTGTG TCCAGAAATG GCCAAACAAG GGAACACGCT 
CTTCTGGCCT ACACCATGGG GGTCAAGCAA CTGATCGTCT GCGTGAACAA AATGGATCTG 
ACGGACCCTC CCTACAGCCA CAAGAGATTT GATGAAGTTG TCAGGAATGT GATGGTCTAT 
CTGAAAAAGA TTGGGTACAA CCCGGCTACC ATCCCCTTCG TGCCCGTGTC TGGCTGGACG 
GGAGAGAATA TATCTTCGCC CAGTCAAAAG ATGGGTTGGT TTAAAGGTTG GAAGGTGAAA 
CGAAAAGATG GCTTTACAAA GGGCCAATCC CTCTTGGAGG TTCTGGATGC GCTTGTACCT 
CCAGTGAGGC CGGCAAATAA GCCTTTGCGG CTCCCCCTGC AGGATGTATA CAAGATAGGG 
GGCATTGGTA CAGTCCCTGT GGGCAAGATA GAAACTGGGA TTCTGAAGCC AGGCATGACC 
ATCTCCTTTG CACCGTCTGG TTTCTCAGCT GAAGTTAAAT CCATAGAAAT GCACCACGAG 
CCGCTTCAGA TGGCCTTCCC AGGGTTCAAC ATCGGATTCA ATGTCAAGAA CATTGCTGTG 
AAAAGTCTAA AGCGTGGCAA TGTGGCGGGC AATTCAAAGA GTGACCCACC GACTGAGGCC 
TCCAGCTTCA CTGCCCAGGT GATCATTCTG AACCACCCGG GCTTTATCAA AGCCGGATAT 
TCACCGGTTA TCGACTGTCA CACTGCACAC ATCACATGCC AGTTTGCAGA ACTGCAGGAA 
AAGATTGACA GGCGGACTGG CAAAAAGCTA GAGGACAACC CGGGGCTACT GAAATCTGGA 
GATGCCGCCA TCATAACCCT GAAGCCCATC AAGCCCTTCT GTGTGGAGAG GTTCTTTGAT 
TATCCACCTC TAGGGAGGTT TGCAGCCCGA GACCTAAAAC AGACTGTTGC CGTCGGGGTT 
GTGAAGTCGG TGGAGCACAA AGCTGGAGCT GCTGCCAGGA GACAAGTCCA AAAACCAGTG 
TTGGTGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes.
The FEBS journal277(22)4722-31(2010 Nov)
Loeber J,Claussen M,Jahn O,Pieler T

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

Correction of the nucleotide and amino acid sequence of Xenopus laevis 42Sp50.
Nucleic acids research21(9)2259(1993 May)
Cheng FM,Darby MK,Joho KE

Structural and functional properties of thesaurin a (42Sp50), the major protein of the 42 S particles present in Xenopus laevis previtellogenic oocytes.
The Journal of biological chemistry266(16)10392-9(1991 Jun)
Viel A,le Maire M,Philippe H,Morales J,Mazabraud A,Denis H

Three genes under different developmental control encode elongation factor 1-alpha in Xenopus laevis.
Nucleic acids research18(12)3489-93(1990 Jun)
Dj? MK,Mazabraud A,Viel A,le Maire M,Denis H,Crawford E,Brown DD