LOC111248458 cDNA ORF clone, Varroa destructor(honeybee mite)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC111248458
Entrez Gene ID	111248458
Full Name	probable leucine--tRNA ligase, mitochondrial
Gene Type	protein-coding
Organism	Varroa destructor(honeybee mite)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022800879.1	XP_022656614.1	probable leucine--tRNA ligase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following LOC111248458 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC111248458 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OVa77035	XM_022800879.1 Latest version!	Varroa destructor probable leucine--tRNA ligase, mitochondrial (LOC111248458), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC111248458 ( XM_022800879.1 ) cDNA ORF clone, Varroa destructor(honeybee mite) -> XP_022656614.1 Varroa destructor probable leucine--tRNA ligase, mitochondrial (LOC111248458), mRNA.

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CloneID	OVa77035
Clone ID Related Accession (Same CDS sequence)	XM_022800879.1
Accession Version	XM_022800879.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Varroa destructor(honeybee mite)
Product	probable leucine--tRNA ligase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019211456.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Varroa destructor Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATTC TGCAGTTTAA CAAGCCTGCG CAATTCGTAC GAACACTGTT TAGTCGGAGC 
GGAGAGTGGG GCGCAGCGCT TACGCGCGAT GTTAAGAAGG ACATCGAATC ATTCTGGAAA 
CAAAAGGTGG CTGAAAGAAA GACGCATCGT TCGAATGAGA AGATGTATGT GCTTTCTATG 
TTTCCCTATC CATCCGGAAA CCTTCACATG GGGCACGTTC GGGTGTACAC AATTGGAGAC 
TTGTTGGCCC GTTATTACAG CATGCGAGGA AAGAACGTCA TTTACCCCAT TGGGTGGGAT 
AGTTTTGGAT TGCCCGCAGA AAATGCAGCT ATTGAACACG GTGAAGATCC CAAGGAATGG 
ACAGAAAAGA ATATTGCCAG TATGACCCGC CAGTTTCGGG AATTTAACTA CAGTTTTAAT 
TGGGAACGAG AAATCTCAAC CTGCGATCCA ACCTATTATC GCTGGACTCA GTTTCTCTTC 
ATAAAATTGT TTGAAAAAGG GCTTGTGTAC CGTAAAAAAG CCGTTGTCAA TTGGGATCCT 
ATAGATAATA CAACTCTAGC CGACGAACAA GTTGATTCCG ACGGACGCAG CTGGAGATCT 
GGAGCTAAAG TTGAAAGACG ACTACTTGAC CAATGGTTTG TACGCACGAC GCAATACTCA 
AGAGATCTTT TTCAGGGTTT GGATGAACTC CAAGGGTGGG GCGACATTAT AAGCATTCAA 
AAAGGCTGGA TTGGAGATGT AGATAGGGGA ATTCTCTTTA CATTTGGTCT TGATGAAGGA 
TTGCCTATAA ATAAGCTAAG CGTATGGACC GATCGGCCAG AGTTAATGGT TGGAGCGGCA 
TTTATTATAA TTAGCGAAGA CCACTTACTA GCACAGGCGC TGAGTACGAA ACAAATATAT 
GCAAGAAATC CATTTGACGA GAATCGGCGT ATACCTGTAT TTGTCAATGA AGACGATTTC 
GATGTTCCCA AAGCGACAGG TGCCTTCGTT GGGGTGCCAT CTTCTTCGGA TGATCATGCA 
AGGTTTGCCA GAATGAAATA TATTGACTTC CTAAATGTCA TCGATGGGGA TCAAATTGTC 
AACTCAGGAA AATTTAATGG ACCAATACTG GAAGCCCGTC GAAAAATATT AGATTTTGCA 
AAAACTGCTA AGATCGGAGG ATTTCCTGCA AGCAAGTGCT TAAGAGACTG GCTAATTTCC 
CGCCAGAGGT ATTGGGGCAC CCCAATACCT ATTGTCCATT GTGAGAAATG CGGACCTGTG 
CCAGTTAGAG AAAAGGATCT CCCAGTACAG TTACCAGCAC TGCCCAAGGG TAAAAAGCTA 
GCCGATATTC ATGACTGGAT TTATACATCG TGTCCTAAAT GTAGAAGAAC CAGCAAAAGG 
GAAACTGACA CATTGGACAC GTTCGTAGAT AGTTCATGGT ACTTCATGAG ATTTCTGGAT 
CCAACAAACG CAAAAAAACT CGTAGACCCA TTAATATCCA ACAGGATGAT GCCTGTGGAT 
ATGTATATTG GTGGATTAGA ACACGCGGTC ATGCATCTTT ACTATGCGAG GTTTATGGCA 
TACTTCATGC ATAGAGAGCT TGGTTTGGGG TGCTCTGATA GTCCTGAACC TTTTAAGAGA 
ATGATTGTAC AAGGAATGGT CCTCAACGAG ACTTTCCGAA TTGAAGCAAC AGGCGAACAT 
GTGCCCAAAG ATCGAGTTGA TTTCAGCGAT TCAAAGAACC TGAAAACAAT AGATACTGGT 
TTGCCTGTTT CTCTATACTG GGATAAAATG TCTAAATCAA AGCACAATGG TATTGCACCT 
CAGGATATCA CAGACATGTA TGGAATTGAC ACAACGCGCC TCTTCATCCT TGGTGACTGC 
GCACCCACTT CACGCTTCAA GTGGCCAGGT ATAGCGTTTA AAGGTATTGC TGGTTGGCAG 
GATAAACTGT GGATGACAGT CGGGAATTTC CGTCTGTCCA GGACAGTTGC AAAAGAGACT 
ATCAATACTG ATCATGAACG TCGCTGTCAG CAAGCTCGAA ACGAGTTTGT TAAGAAGTGC 
AACTTTAACT TTGAAGTGAC CCACCAGTTC AATCTCATTA TTAGAGATAT GCAAATCTTT 
ACAAATACTC TTCGCAGTGG ATTACGAAGC CCTCAGACTT TCCTCGGACC AAAATATGAA 
CGCAGCCTCG GAGACCTATT AATTTGCTTA GCACCATTTT GTCCACACTT CGCATCTGAG 
TTGTGGGCTG GATTCTTCGA TAAAGTAGAA TATAACAAGA ACACATTTCA GGACGGCGGA 
GTTTTTGCTC AGAAATGGCC TCAACTTGAC GACGATGCTT TGTTTGATCT TGTTGTTAAC 
GTTGGCAAGA GTCAAATGCT AACAGTAAAA TTAACCAAGA ATCTTTTTGT TGGTCTAACA 
AAGGAGCGTG CTATACAACT TGTAAACAAA AACTTGCCAA TAGAGTTTAG CGAGGCTAGC 
CCTAAATTTA GTTACTTCCC TTTATTCAGA GCTGAACTAA ACTATGTAAA TATTAGATAT 
AATAATGTGA AAAAGGAAAA AAACAAAATA AGAAAGCACT TGCACGATAA TAGCTCAAAT 
ATTAGTAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022656614.1
CDS	347..2938
Translation	MSILQFNKPAQFVRTLFSRSGEWGAALTRDVKKDIESFWKQKVAERKTHRSNEKMYVLSMFPYPSGNLHMGHVRVYTIGDLLARYYSMRGKNVIYPIGWDSFGLPAENAAIEHGEDPKEWTEKNIASMTRQFREFNYSFNWEREISTCDPTYYRWTQFLFIKLFEKGLVYRKKAVVNWDPIDNTTLADEQVDSDGRSWRSGAKVERRLLDQWFVRTTQYSRDLFQGLDELQGWGDIISIQKGWIGDVDRGILFTFGLDEGLPINKLSVWTDRPELMVGAAFIIISEDHLLAQALSTKQIYARNPFDENRRIPVFVNEDDFDVPKATGAFVGVPSSSDDHARFARMKYIDFLNVIDGDQIVNSGKFNGPILEARRKILDFAKTAKIGGFPASKCLRDWLISRQRYWGTPIPIVHCEKCGPVPVREKDLPVQLPALPKGKKLADIHDWIYTSCPKCRRTSKRETDTLDTFVDSSWYFMRFLDPTNAKKLVDPLISNRMMPVDMYIGGLEHAVMHLYYARFMAYFMHRELGLGCSDSPEPFKRMIVQGMVLNETFRIEATGEHVPKDRVDFSDSKNLKTIDTGLPVSLYWDKMSKSKHNGIAPQDITDMYGIDTTRLFILGDCAPTSRFKWPGIAFKGIAGWQDKLWMTVGNFRLSRTVAKETINTDHERRCQQARNEFVKKCNFNFEVTHQFNLIIRDMQIFTNTLRSGLRSPQTFLGPKYERSLGDLLICLAPFCPHFASELWAGFFDKVEYNKNTFQDGGVFAQKWPQLDDDALFDLVVNVGKSQMLTVKLTKNLFVGLTKERAIQLVNKNLPIEFSEASPKFSYFPLFRAELNYVNIRYNNVKKEKNKIRKHLHDNSSNISS

Target ORF information:

RefSeq Version	XM_022800879.1
Organism	Varroa destructor(honeybee mite)
Definition	Varroa destructor probable leucine--tRNA ligase, mitochondrial (LOC111248458), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022800879.1

ORF Insert Sequence:

ATGAGCATTC TGCAGTTTAA CAAGCCTGCG CAATTCGTAC GAACACTGTT TAGTCGGAGC 
GGAGAGTGGG GCGCAGCGCT TACGCGCGAT GTTAAGAAGG ACATCGAATC ATTCTGGAAA 
CAAAAGGTGG CTGAAAGAAA GACGCATCGT TCGAATGAGA AGATGTATGT GCTTTCTATG 
TTTCCCTATC CATCCGGAAA CCTTCACATG GGGCACGTTC GGGTGTACAC AATTGGAGAC 
TTGTTGGCCC GTTATTACAG CATGCGAGGA AAGAACGTCA TTTACCCCAT TGGGTGGGAT 
AGTTTTGGAT TGCCCGCAGA AAATGCAGCT ATTGAACACG GTGAAGATCC CAAGGAATGG 
ACAGAAAAGA ATATTGCCAG TATGACCCGC CAGTTTCGGG AATTTAACTA CAGTTTTAAT 
TGGGAACGAG AAATCTCAAC CTGCGATCCA ACCTATTATC GCTGGACTCA GTTTCTCTTC 
ATAAAATTGT TTGAAAAAGG GCTTGTGTAC CGTAAAAAAG CCGTTGTCAA TTGGGATCCT 
ATAGATAATA CAACTCTAGC CGACGAACAA GTTGATTCCG ACGGACGCAG CTGGAGATCT 
GGAGCTAAAG TTGAAAGACG ACTACTTGAC CAATGGTTTG TACGCACGAC GCAATACTCA 
AGAGATCTTT TTCAGGGTTT GGATGAACTC CAAGGGTGGG GCGACATTAT AAGCATTCAA 
AAAGGCTGGA TTGGAGATGT AGATAGGGGA ATTCTCTTTA CATTTGGTCT TGATGAAGGA 
TTGCCTATAA ATAAGCTAAG CGTATGGACC GATCGGCCAG AGTTAATGGT TGGAGCGGCA 
TTTATTATAA TTAGCGAAGA CCACTTACTA GCACAGGCGC TGAGTACGAA ACAAATATAT 
GCAAGAAATC CATTTGACGA GAATCGGCGT ATACCTGTAT TTGTCAATGA AGACGATTTC 
GATGTTCCCA AAGCGACAGG TGCCTTCGTT GGGGTGCCAT CTTCTTCGGA TGATCATGCA 
AGGTTTGCCA GAATGAAATA TATTGACTTC CTAAATGTCA TCGATGGGGA TCAAATTGTC 
AACTCAGGAA AATTTAATGG ACCAATACTG GAAGCCCGTC GAAAAATATT AGATTTTGCA 
AAAACTGCTA AGATCGGAGG ATTTCCTGCA AGCAAGTGCT TAAGAGACTG GCTAATTTCC 
CGCCAGAGGT ATTGGGGCAC CCCAATACCT ATTGTCCATT GTGAGAAATG CGGACCTGTG 
CCAGTTAGAG AAAAGGATCT CCCAGTACAG TTACCAGCAC TGCCCAAGGG TAAAAAGCTA 
GCCGATATTC ATGACTGGAT TTATACATCG TGTCCTAAAT GTAGAAGAAC CAGCAAAAGG 
GAAACTGACA CATTGGACAC GTTCGTAGAT AGTTCATGGT ACTTCATGAG ATTTCTGGAT 
CCAACAAACG CAAAAAAACT CGTAGACCCA TTAATATCCA ACAGGATGAT GCCTGTGGAT 
ATGTATATTG GTGGATTAGA ACACGCGGTC ATGCATCTTT ACTATGCGAG GTTTATGGCA 
TACTTCATGC ATAGAGAGCT TGGTTTGGGG TGCTCTGATA GTCCTGAACC TTTTAAGAGA 
ATGATTGTAC AAGGAATGGT CCTCAACGAG ACTTTCCGAA TTGAAGCAAC AGGCGAACAT 
GTGCCCAAAG ATCGAGTTGA TTTCAGCGAT TCAAAGAACC TGAAAACAAT AGATACTGGT 
TTGCCTGTTT CTCTATACTG GGATAAAATG TCTAAATCAA AGCACAATGG TATTGCACCT 
CAGGATATCA CAGACATGTA TGGAATTGAC ACAACGCGCC TCTTCATCCT TGGTGACTGC 
GCACCCACTT CACGCTTCAA GTGGCCAGGT ATAGCGTTTA AAGGTATTGC TGGTTGGCAG 
GATAAACTGT GGATGACAGT CGGGAATTTC CGTCTGTCCA GGACAGTTGC AAAAGAGACT 
ATCAATACTG ATCATGAACG TCGCTGTCAG CAAGCTCGAA ACGAGTTTGT TAAGAAGTGC 
AACTTTAACT TTGAAGTGAC CCACCAGTTC AATCTCATTA TTAGAGATAT GCAAATCTTT 
ACAAATACTC TTCGCAGTGG ATTACGAAGC CCTCAGACTT TCCTCGGACC AAAATATGAA 
CGCAGCCTCG GAGACCTATT AATTTGCTTA GCACCATTTT GTCCACACTT CGCATCTGAG 
TTGTGGGCTG GATTCTTCGA TAAAGTAGAA TATAACAAGA ACACATTTCA GGACGGCGGA 
GTTTTTGCTC AGAAATGGCC TCAACTTGAC GACGATGCTT TGTTTGATCT TGTTGTTAAC 
GTTGGCAAGA GTCAAATGCT AACAGTAAAA TTAACCAAGA ATCTTTTTGT TGGTCTAACA 
AAGGAGCGTG CTATACAACT TGTAAACAAA AACTTGCCAA TAGAGTTTAG CGAGGCTAGC 
CCTAAATTTA GTTACTTCCC TTTATTCAGA GCTGAACTAA ACTATGTAAA TATTAGATAT 
AATAATGTGA AAAAGGAAAA AAACAAAATA AGAAAGCACT TGCACGATAA TAGCTCAAAT 
ATTAGTAGTT AG