SPHK2 cDNA ORF clone, Ursus maritimus(polar bear)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPHK2
Entrez Gene ID	103657238
Full Name	sphingosine kinase 2
Gene Type	protein-coding
Organism	Ursus maritimus(polar bear)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008684664.1	XP_008682886.1	sphingosine kinase 2

Pathways from BioSystems
Gene Ontology

The following SPHK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPHK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OUr02768	XM_008684664.1 Latest version!	Ursus maritimus sphingosine kinase 2 (SPHK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPHK2 ( XM_008684664.1 ) cDNA ORF clone, Ursus maritimus(polar bear) -> XP_008682886.1 Ursus maritimus sphingosine kinase 2 (SPHK2), mRNA.

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CloneID	OUr02768
Clone ID Related Accession (Same CDS sequence)	XM_008684664.1
Accession Version	XM_008684664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-08-03
Organism	Ursus maritimus(polar bear)
Product	sphingosine kinase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007907048.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ursus maritimus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CGGGATCAGA GGCCAGACCA GGAGCTGACC 
TGGAGCTGGG GCCACAGGCC TAGAAGCGCC CTGGACAGGG CTGAGGCCAT GGCCCCCCCA 
CCACCGCCGG GCCGGCGCAG AGCCGCCCGC ACTTTTCGGG CCGACGGGGC AGCCACCTAC 
GAGGAGAACC GCGCTGAGGC CCAGCGCTGG GCCATTGCCC TCATGTGTCT GCTCCGTGGA 
CTGCCACTAC CTGGGGACGG GGAAATAACC CCCGAACTGC TGCCAAGGCC CCCCCGATTG 
CTCCTATTGG TCAACCCCTT CGGGGGGCGG GGTCTGGCCT GGCAATGGTG TAAGAACCAT 
GTGCTGCCCA TGATCTCTGA AGCTGGGCTG TCCTTCAACC TCATCCAGAC AGAGCGGCAG 
AACCATGCCC GGGAGCTGGT TCAGGGGCTG AGCCTGAGCG AGTGGGACGG CATCATCACA 
GTCTCGGGAG ACGGGCTGCT GTTCGAGGTG CTGAATGGAC TCCTAGACCG CCCTGACTGG 
GAAGAAGCTG TGAAGACACC CGTGGGCATC CTCCCCTGTG GCTCAGGCAA TGCCCTGGCT 
GGAGCCGTGA ACCAACATGG GGGGTTTGAA CCTGCCCTGG GTGTCGACCT GCTGCTCAAC 
TGCTCGCTGC TCCTTTGCCG GGGTGGCAGC CGCCCGCTGG ATCTGCTCTC TGTGACGCTG 
GCCTCGGGCT CCCGCTGTTT CTCCTTCCTG TCCGTGGCCT GGGGCTTCGT GTCAGACGTG 
GACATCCAGA GTGAGCGCTT CAGGGCCCTG GGCAGCGCCC GCTTCACACT GGGCACGGTG 
CTGGGCCTCG CCAACCTGCA CACCTACCGC GGACGCCTCT CCACTGTGGC ACACTCACCC 
CTGCATCGCT CGGTGTCTGA CCTGCCTCTG CCCTTGCCCC TGCCCAAGCC TGCCCTGACC 
TCCCCTGGCT CACCTGAGCC CCTGCCCATC CTGTCCCTCA ATGGTGGGGG CCCAGAGTTG 
GCCGGAGACT GGGGTGGGGC TGGGGATGCC CCACTGTCCC CAGACCCCCT GCTGCCCTCA 
CCACCTAGCT CCCCTAAGGC TTTACTGTCA CCCATCACCG AGGGGGTCCC AGAAATGCCA 
ACCACCTCTG GGCTCCCACC TCCCACCCCT GATGCCCCAG GAGCCATCTC TACCAGGGGC 
CCACCTGACC ACCTGCTCCC TGCTCTGGGC ACCCCACTCC CCCCAGGCTG GGTGACGCTG 
GAGGGGGACT TTGTGCTCAT GTTGGCCATC TCACCCAGCC ACCTGGGGGC TGACCTGGTG 
GCAGCTCCCC ATGCGCGCTT TGACGACGGC CTGGTGCACC TGTGCTGGGT GCGTGGGGGC 
ATCTCGCGGG CTGCGCTGCT GCGCCTTTTT CTGGCCATGG AGCGAGGAGG CCACTTCAGC 
CTGGGCTGTC CGCAGCTGGG CTACGCCGCG GCCCGTGCCT TCCGCCTGGA GCCTCTCACG 
CCTCGCGGCG TGCTCACTGT GGACGGAGAG CAGGTGGAGT ATGGGCCACT GCAGGCGCAG 
GTGCATCCAG GCCTCGGTAC ACTGCTCACT GGGCCTCCGG GCCGCCCGGG TCGGGAGCCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008682886.1
CDS	370..1992
Translation	MNGHLEAEEQRDQRPDQELTWSWGHRPRSALDRAEAMAPPPPPGRRRAARTFRADGAATYEENRAEAQRWAIALMCLLRGLPLPGDGEITPELLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLSFNLIQTERQNHARELVQGLSLSEWDGIITVSGDGLLFEVLNGLLDRPDWEEAVKTPVGILPCGSGNALAGAVNQHGGFEPALGVDLLLNCSLLLCRGGSRPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERFRALGSARFTLGTVLGLANLHTYRGRLSTVAHSPLHRSVSDLPLPLPLPKPALTSPGSPEPLPILSLNGGGPELAGDWGGAGDAPLSPDPLLPSPPSSPKALLSPITEGVPEMPTTSGLPPPTPDAPGAISTRGPPDHLLPALGTPLPPGWVTLEGDFVLMLAISPSHLGADLVAAPHARFDDGLVHLCWVRGGISRAALLRLFLAMERGGHFSLGCPQLGYAAARAFRLEPLTPRGVLTVDGEQVEYGPLQAQVHPGLGTLLTGPPGRPGREP

Target ORF information:

RefSeq Version	XM_008684664.1
Organism	Ursus maritimus(polar bear)
Definition	Ursus maritimus sphingosine kinase 2 (SPHK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008684664.1

ORF Insert Sequence:

ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CGGGATCAGA GGCCAGACCA GGAGCTGACC 
TGGAGCTGGG GCCACAGGCC TAGAAGCGCC CTGGACAGGG CTGAGGCCAT GGCCCCCCCA 
CCACCGCCGG GCCGGCGCAG AGCCGCCCGC ACTTTTCGGG CCGACGGGGC AGCCACCTAC 
GAGGAGAACC GCGCTGAGGC CCAGCGCTGG GCCATTGCCC TCATGTGTCT GCTCCGTGGA 
CTGCCACTAC CTGGGGACGG GGAAATAACC CCCGAACTGC TGCCAAGGCC CCCCCGATTG 
CTCCTATTGG TCAACCCCTT CGGGGGGCGG GGTCTGGCCT GGCAATGGTG TAAGAACCAT 
GTGCTGCCCA TGATCTCTGA AGCTGGGCTG TCCTTCAACC TCATCCAGAC AGAGCGGCAG 
AACCATGCCC GGGAGCTGGT TCAGGGGCTG AGCCTGAGCG AGTGGGACGG CATCATCACA 
GTCTCGGGAG ACGGGCTGCT GTTCGAGGTG CTGAATGGAC TCCTAGACCG CCCTGACTGG 
GAAGAAGCTG TGAAGACACC CGTGGGCATC CTCCCCTGTG GCTCAGGCAA TGCCCTGGCT 
GGAGCCGTGA ACCAACATGG GGGGTTTGAA CCTGCCCTGG GTGTCGACCT GCTGCTCAAC 
TGCTCGCTGC TCCTTTGCCG GGGTGGCAGC CGCCCGCTGG ATCTGCTCTC TGTGACGCTG 
GCCTCGGGCT CCCGCTGTTT CTCCTTCCTG TCCGTGGCCT GGGGCTTCGT GTCAGACGTG 
GACATCCAGA GTGAGCGCTT CAGGGCCCTG GGCAGCGCCC GCTTCACACT GGGCACGGTG 
CTGGGCCTCG CCAACCTGCA CACCTACCGC GGACGCCTCT CCACTGTGGC ACACTCACCC 
CTGCATCGCT CGGTGTCTGA CCTGCCTCTG CCCTTGCCCC TGCCCAAGCC TGCCCTGACC 
TCCCCTGGCT CACCTGAGCC CCTGCCCATC CTGTCCCTCA ATGGTGGGGG CCCAGAGTTG 
GCCGGAGACT GGGGTGGGGC TGGGGATGCC CCACTGTCCC CAGACCCCCT GCTGCCCTCA 
CCACCTAGCT CCCCTAAGGC TTTACTGTCA CCCATCACCG AGGGGGTCCC AGAAATGCCA 
ACCACCTCTG GGCTCCCACC TCCCACCCCT GATGCCCCAG GAGCCATCTC TACCAGGGGC 
CCACCTGACC ACCTGCTCCC TGCTCTGGGC ACCCCACTCC CCCCAGGCTG GGTGACGCTG 
GAGGGGGACT TTGTGCTCAT GTTGGCCATC TCACCCAGCC ACCTGGGGGC TGACCTGGTG 
GCAGCTCCCC ATGCGCGCTT TGACGACGGC CTGGTGCACC TGTGCTGGGT GCGTGGGGGC 
ATCTCGCGGG CTGCGCTGCT GCGCCTTTTT CTGGCCATGG AGCGAGGAGG CCACTTCAGC 
CTGGGCTGTC CGCAGCTGGG CTACGCCGCG GCCCGTGCCT TCCGCCTGGA GCCTCTCACG 
CCTCGCGGCG TGCTCACTGT GGACGGAGAG CAGGTGGAGT ATGGGCCACT GCAGGCGCAG 
GTGCATCCAG GCCTCGGTAC ACTGCTCACT GGGCCTCCGG GCCGCCCGGG TCGGGAGCCT 
TGA