BRAF cDNA ORF clone, Tupaia chinensis(Chinese tree shrew)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAF
Entrez Gene ID	102477389
Full Name	B-Raf proto-oncogene, serine/threonine kinase
Gene Type	protein-coding
Organism	Tupaia chinensis(Chinese tree shrew)

mRNA and Protein(s)

mRNA	Protein	Name
XM_027770524.1	XP_027626325.1	serine/threonine-protein kinase B-raf isoform X3
XM_027770525.1	XP_027626326.1	serine/threonine-protein kinase B-raf isoform X4
XM_014586455.1	XP_014441941.1	serine/threonine-protein kinase B-raf
XM_027770523.1	XP_027626324.1	serine/threonine-protein kinase B-raf isoform X2
XM_027770526.1	XP_027626327.1	serine/threonine-protein kinase B-raf isoform X5
XM_027770522.1	XP_027626323.1	serine/threonine-protein kinase B-raf isoform X1

Pathways from BioSystems
Gene Ontology

The following BRAF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTu04881	XM_014586455.1 Latest version!	Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OTu63108	XM_027770522.1 Latest version!	Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTu63109	XM_027770523.1 Latest version!	Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTu63110	XM_027770524.1 Latest version!	Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTu63111	XM_027770525.1 Latest version!	Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTu63112	XM_027770526.1 Latest version!	Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAF ( XM_014586455.1 ) cDNA ORF clone, Tupaia chinensis(Chinese tree shrew) -> XP_014441941.1 Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA.

Price & Availability↑

CloneID	OTu04881
Clone ID Related Accession (Same CDS sequence)	XM_014586455.1
Accession Version	XM_014586455.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-03
Organism	Tupaia chinensis(Chinese tree shrew)
Product	serine/threonine-protein kinase B-raf
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006159610.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tupaia chinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCTCTGG TGTGGAATAT CAAACAAATG ATAAAGTTGA CACAGGAACA TATAGAGGCC 
CTATTGGACA AATTTGGTGG GGAGCATAAT CCACCATCAA TATATCTGGA GGCCTATGAA 
GAATACACCA GCAAGCTAGA TGCCCTCCAA CAAAGAGAAC AACAGTTATT GGAATCCCTG 
GGGAATGGAA CTGATTTTTC TGTTTCTAGC TCTGCATCAA CAGACACCGT TACATCTTCT 
TCCTCTTCTA GCCTTTCAGT GCTACCTTCA TCTCTTTCAG TTTTTCAAAA TCCCACAGAT 
GCGTCACGGA GTAACCCCAA GTCACCACAA AAACCTATTG TTAGAGTCTT CCTGCCCAAC 
AAACAGAGGA CAGTGGTACC TGCAAGGTGT GGAGTTACAG TTCGAGACAG TCTAAAGAAA 
GCACTGATGA TGAGAGGTCT AATCCCAGAG TGCTGTGCTG TGTACAGAAT TCAGGATGGA 
GAGAAGAAAC CAATTGGCTG GGACACTGAT ATTTCTTGGC TTACTGGAGA AGAATTACAT 
GTGGAAGTGT TGGAAAATGT ACCACTTACA ACACATAACT TTGTACGGAA AACTTTTTTC 
ACCTTAGCGT TTTGTGACTT TTGTCGAAAG CTGCTTTTCC AGGGTTTCCG CTGTCAAACA 
TGTGGTTATA AATTTCACCA GCGTTGTAGT ACAGAGGTTC CACTGATGTG TGTTAATTAT 
GACCAACTTG ATTTGCTGTT TGTCTCCAAG TTCTTTGAAC ACCACCCAAT ACCACAGGAA 
GAGACCTCCT TGGCAGAGAC TGCCCTTACA TCTGGAGCAT CCCCTTCTGC ACCTCCCTCA 
GACTCTCTTG GGCCCCAAAT TCTTACCAGT CCGTCTCCTT CAAAATCCAT TCCAATCCCA 
CAGCCCTTCC GACCAGCAGA TGAAGATCAT CGAAATCAAT TTGGGCAACG AGACCGATCC 
TCATCAGCTC CCAACGTGCA TATAAATACA ATAGAACCTG TCAATATTGA AGAAAAATTC 
CCAGAAGTGG AATTACAGGA TCAAAGGGAC TTGATTAGAG ACCAAGGGTT TCGTGGTGAT 
GGAGCCCCTT TGAACCAGCT GATGCGCTGT CTTCGGAAAT ACCAATCCCG GACTCCCAGT 
CCCCTCCTAC ATTCTGTCCC CAGTGAAATA GTGTTTGATT TTGAGCCTGG CCCAGTGTTC 
AGAGGATCAA CCACAGGTTT GTCTGCTACG CCCCCTGCCT CATTACCTGG CTCACTCACT 
AATGTGAAAG CCTTACAGAA ATCTCCAGGA CCTCAGAGAG AAAGGAAGTC ATCTTCATCC 
TCCGAAGACA GGAATCGAAT GAAAACACTT GGTAGACGAG ACTCAAGTGA TGATTGGGAG 
ATTCCTGATG GGCAGATTAC AGTGGGACAA AGAATTGGAT CTGGGTCATT TGGAACAGTC 
TACAAGGGAA AGTGGCATGG TGATGTGGCA GTGAAAATGT TGAATGTGAC AGCACCCACA 
CCTCAGCAGT TACAGGCCTT CAAAAATGAA GTAGGAGTAC TCAGGAAAAC ACGACATGTA 
AATATCCTAC TTTTCATGGG CTATTCCACA AAGCCACAGC TGGCCATCGT TACCCAGTGG 
TGTGAAGGCT CCAGCTTATA TCACCATCTC CACATCATTG AGACCAAATT TGAGATGATA 
AAGCTTATAG ATATTGCACG GCAGACTGCA CAGGGCATGG ATTATTTACA CGCCAAGTCA 
ATCATCCACA GAGACCTCAA GAGTAATAAT ATATTTCTTC ATGAAGACCT CACAGTAAAA 
ATAGGTGACT TTGGTTTAGC CACAGTGAAA TCTCGATGGA GTGGGTCCCA TCAGTTTGAA 
CAGTTGTCTG GTTCCATTTT GTGGATGGCA CCAGAAGTGA TCAGAATGCA AGATAAAAAT 
CCATATAGCT TTCAGTCGGA TGTGTATGCA TTTGGGATTG TTTTGTATGA ATTGATGACT 
GGACAGTTAC CTTATTCAAA TATCAACAAC AGGGACCAGA TAATTTTTAT GGTGGGACGA 
GGATACCTAT CTCCAGATCT CAGTAAGGTA CGGAGTAACT GTCCAAAAGC CATGAAGAGA 
TTAATGGCAG AGTGCCTCAA AAAGAAAAGA GATGAGAGAA AACTCTTTCC TCAGATTCTC 
GCCTCTATTG AGCTGCTGGC CCGCTCATTG CCAAAAATTC ACCGCAGTGC ATCAGAACCC 
TCCTTGAATC GGGCTGGTTT CCAAACAGAG GATTTTAGTC TATATGCTTG TGCTTCTCCA 
AAAACACCCA TCCAGGCAGG GGGATATGGA GAATTTGCAG CCTTCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014441941.1
CDS	1..2331
Translation	MSLVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASTDTVTSSSSSSLSVLPSSLSVFQNPTDASRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEETSLAETALTSGASPSAPPSDSLGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIEEKFPEVELQDQRDLIRDQGFRGDGAPLNQLMRCLRKYQSRTPSPLLHSVPSEIVFDFEPGPVFRGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERKLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK

Target ORF information:

RefSeq Version	XM_014586455.1
Organism	Tupaia chinensis(Chinese tree shrew)
Definition	Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014586455.1

ORF Insert Sequence:

ATGTCTCTGG TGTGGAATAT CAAACAAATG ATAAAGTTGA CACAGGAACA TATAGAGGCC 
CTATTGGACA AATTTGGTGG GGAGCATAAT CCACCATCAA TATATCTGGA GGCCTATGAA 
GAATACACCA GCAAGCTAGA TGCCCTCCAA CAAAGAGAAC AACAGTTATT GGAATCCCTG 
GGGAATGGAA CTGATTTTTC TGTTTCTAGC TCTGCATCAA CAGACACCGT TACATCTTCT 
TCCTCTTCTA GCCTTTCAGT GCTACCTTCA TCTCTTTCAG TTTTTCAAAA TCCCACAGAT 
GCGTCACGGA GTAACCCCAA GTCACCACAA AAACCTATTG TTAGAGTCTT CCTGCCCAAC 
AAACAGAGGA CAGTGGTACC TGCAAGGTGT GGAGTTACAG TTCGAGACAG TCTAAAGAAA 
GCACTGATGA TGAGAGGTCT AATCCCAGAG TGCTGTGCTG TGTACAGAAT TCAGGATGGA 
GAGAAGAAAC CAATTGGCTG GGACACTGAT ATTTCTTGGC TTACTGGAGA AGAATTACAT 
GTGGAAGTGT TGGAAAATGT ACCACTTACA ACACATAACT TTGTACGGAA AACTTTTTTC 
ACCTTAGCGT TTTGTGACTT TTGTCGAAAG CTGCTTTTCC AGGGTTTCCG CTGTCAAACA 
TGTGGTTATA AATTTCACCA GCGTTGTAGT ACAGAGGTTC CACTGATGTG TGTTAATTAT 
GACCAACTTG ATTTGCTGTT TGTCTCCAAG TTCTTTGAAC ACCACCCAAT ACCACAGGAA 
GAGACCTCCT TGGCAGAGAC TGCCCTTACA TCTGGAGCAT CCCCTTCTGC ACCTCCCTCA 
GACTCTCTTG GGCCCCAAAT TCTTACCAGT CCGTCTCCTT CAAAATCCAT TCCAATCCCA 
CAGCCCTTCC GACCAGCAGA TGAAGATCAT CGAAATCAAT TTGGGCAACG AGACCGATCC 
TCATCAGCTC CCAACGTGCA TATAAATACA ATAGAACCTG TCAATATTGA AGAAAAATTC 
CCAGAAGTGG AATTACAGGA TCAAAGGGAC TTGATTAGAG ACCAAGGGTT TCGTGGTGAT 
GGAGCCCCTT TGAACCAGCT GATGCGCTGT CTTCGGAAAT ACCAATCCCG GACTCCCAGT 
CCCCTCCTAC ATTCTGTCCC CAGTGAAATA GTGTTTGATT TTGAGCCTGG CCCAGTGTTC 
AGAGGATCAA CCACAGGTTT GTCTGCTACG CCCCCTGCCT CATTACCTGG CTCACTCACT 
AATGTGAAAG CCTTACAGAA ATCTCCAGGA CCTCAGAGAG AAAGGAAGTC ATCTTCATCC 
TCCGAAGACA GGAATCGAAT GAAAACACTT GGTAGACGAG ACTCAAGTGA TGATTGGGAG 
ATTCCTGATG GGCAGATTAC AGTGGGACAA AGAATTGGAT CTGGGTCATT TGGAACAGTC 
TACAAGGGAA AGTGGCATGG TGATGTGGCA GTGAAAATGT TGAATGTGAC AGCACCCACA 
CCTCAGCAGT TACAGGCCTT CAAAAATGAA GTAGGAGTAC TCAGGAAAAC ACGACATGTA 
AATATCCTAC TTTTCATGGG CTATTCCACA AAGCCACAGC TGGCCATCGT TACCCAGTGG 
TGTGAAGGCT CCAGCTTATA TCACCATCTC CACATCATTG AGACCAAATT TGAGATGATA 
AAGCTTATAG ATATTGCACG GCAGACTGCA CAGGGCATGG ATTATTTACA CGCCAAGTCA 
ATCATCCACA GAGACCTCAA GAGTAATAAT ATATTTCTTC ATGAAGACCT CACAGTAAAA 
ATAGGTGACT TTGGTTTAGC CACAGTGAAA TCTCGATGGA GTGGGTCCCA TCAGTTTGAA 
CAGTTGTCTG GTTCCATTTT GTGGATGGCA CCAGAAGTGA TCAGAATGCA AGATAAAAAT 
CCATATAGCT TTCAGTCGGA TGTGTATGCA TTTGGGATTG TTTTGTATGA ATTGATGACT 
GGACAGTTAC CTTATTCAAA TATCAACAAC AGGGACCAGA TAATTTTTAT GGTGGGACGA 
GGATACCTAT CTCCAGATCT CAGTAAGGTA CGGAGTAACT GTCCAAAAGC CATGAAGAGA 
TTAATGGCAG AGTGCCTCAA AAAGAAAAGA GATGAGAGAA AACTCTTTCC TCAGATTCTC 
GCCTCTATTG AGCTGCTGGC CCGCTCATTG CCAAAAATTC ACCGCAGTGC ATCAGAACCC 
TCCTTGAATC GGGCTGGTTT CCAAACAGAG GATTTTAGTC TATATGCTTG TGCTTCTCCA 
AAAACACCCA TCCAGGCAGG GGGATATGGA GAATTTGCAG CCTTCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_027770522.1 ) cDNA ORF clone, Tupaia chinensis(Chinese tree shrew) -> XP_027626323.1 Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTu63108
Clone ID Related Accession (Same CDS sequence)	XM_027770522.1
Accession Version	XM_027770522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Tupaia chinensis(Chinese tree shrew)
Product	serine/threonine-protein kinase B-raf isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006159610.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Tupaia chinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAAGT TGACACAGGA ACATATAGAG GCCCTATTGG ACAAATTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAGCT AGATGCCCTC 
CAACAAAGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACAGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATGCGTCAC GGAGTAACCC CAAGTCACCA 
CAAAAACCTA TTGTTAGAGT CTTCCTGCCC AACAAACAGA GGACAGTGGT ACCTGCAAGG 
TGTGGAGTTA CAGTTCGAGA CAGTCTAAAG AAAGCACTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTGTACAG AATTCAGGAT GGAGAGAAGA AACCAATTGG CTGGGACACT 
GATATTTCTT GGCTTACTGG AGAAGAATTA CATGTGGAAG TGTTGGAAAA TGTACCACTT 
ACAACACATA ACTTTGTACG GAAAACTTTT TTCACCTTAG CGTTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT CCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAAGAGACCT CCTTGGCAGA GACTGCCCTT 
ACATCTGGAG CATCCCCTTC TGCACCTCCC TCAGACTCTC TTGGGCCCCA AATTCTTACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATC CCACAGCCCT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AATTTGGGCA ACGAGACCGA TCCTCATCAG CTCCCAACGT GCATATAAAT 
ACAATAGAAC CTGTCAATAT TGAAGAAAAA TTCCCAGAAG TGGAATTACA GGATCAAAGG 
GACTTGATTA GAGACCAAGG GTTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
ACGCCCCCTG CCTCATTACC TGGCTCACTC ACTAATGTGA AAGCCTTACA GAAATCTCCA 
GGACCTCAGA GAGAAAGGAA GTCATCTTCA TCCTCCGAAG ACAGGAATCG AATGAAAACA 
CTTGGTAGAC GAGACTCAAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
CAAAGAATTG GATCTGGGTC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
GCAGTGAAAA TGTTGAATGT GACAGCACCC ACACCTCAGC AGTTACAGGC CTTCAAAAAT 
GAAGTAGGAG TACTCAGGAA AACACGACAT GTAAATATCC TACTTTTCAT GGGCTATTCC 
ACAAAGCCAC AGCTGGCCAT CGTTACCCAG TGGTGTGAAG GCTCCAGCTT ATATCACCAT 
CTCCACATCA TTGAGACCAA ATTTGAGATG ATAAAGCTTA TAGATATTGC ACGGCAGACT 
GCACAGGGCA TGGATTATTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ACTTTGGTTT AGCCACAGTG 
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGTTCCAT TTTGTGGATG 
GCACCAGAAG TGATCAGAAT GCAAGATAAA AATCCATATA GCTTTCAGTC GGATGTGTAT 
GCATTTGGGA TTGTTTTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAATATCAAC 
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TATCTCCAGA TCTCAGTAAG 
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
AGAGATGAGA GAAAACTCTT TCCTCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
GGGAGCAATA CCAGCCTCTT TCTACCTCAT TTACTCCGTT TGGATTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027626323.1
CDS	36..2246
Translation	MIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASTDTVTSSSSSSLSVLPSSLSVFQNPTDASRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEETSLAETALTSGASPSAPPSDSLGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIEEKFPEVELQDQRDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERKLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGSNTSLFLPHLLRLDL

Target ORF information:

RefSeq Version	XM_027770522.1
Organism	Tupaia chinensis(Chinese tree shrew)
Definition	Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027770522.1

ORF Insert Sequence:

ATGATAAAGT TGACACAGGA ACATATAGAG GCCCTATTGG ACAAATTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAGCT AGATGCCCTC 
CAACAAAGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACAGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATGCGTCAC GGAGTAACCC CAAGTCACCA 
CAAAAACCTA TTGTTAGAGT CTTCCTGCCC AACAAACAGA GGACAGTGGT ACCTGCAAGG 
TGTGGAGTTA CAGTTCGAGA CAGTCTAAAG AAAGCACTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTGTACAG AATTCAGGAT GGAGAGAAGA AACCAATTGG CTGGGACACT 
GATATTTCTT GGCTTACTGG AGAAGAATTA CATGTGGAAG TGTTGGAAAA TGTACCACTT 
ACAACACATA ACTTTGTACG GAAAACTTTT TTCACCTTAG CGTTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT CCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAAGAGACCT CCTTGGCAGA GACTGCCCTT 
ACATCTGGAG CATCCCCTTC TGCACCTCCC TCAGACTCTC TTGGGCCCCA AATTCTTACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATC CCACAGCCCT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AATTTGGGCA ACGAGACCGA TCCTCATCAG CTCCCAACGT GCATATAAAT 
ACAATAGAAC CTGTCAATAT TGAAGAAAAA TTCCCAGAAG TGGAATTACA GGATCAAAGG 
GACTTGATTA GAGACCAAGG GTTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
ACGCCCCCTG CCTCATTACC TGGCTCACTC ACTAATGTGA AAGCCTTACA GAAATCTCCA 
GGACCTCAGA GAGAAAGGAA GTCATCTTCA TCCTCCGAAG ACAGGAATCG AATGAAAACA 
CTTGGTAGAC GAGACTCAAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
CAAAGAATTG GATCTGGGTC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
GCAGTGAAAA TGTTGAATGT GACAGCACCC ACACCTCAGC AGTTACAGGC CTTCAAAAAT 
GAAGTAGGAG TACTCAGGAA AACACGACAT GTAAATATCC TACTTTTCAT GGGCTATTCC 
ACAAAGCCAC AGCTGGCCAT CGTTACCCAG TGGTGTGAAG GCTCCAGCTT ATATCACCAT 
CTCCACATCA TTGAGACCAA ATTTGAGATG ATAAAGCTTA TAGATATTGC ACGGCAGACT 
GCACAGGGCA TGGATTATTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ACTTTGGTTT AGCCACAGTG 
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGTTCCAT TTTGTGGATG 
GCACCAGAAG TGATCAGAAT GCAAGATAAA AATCCATATA GCTTTCAGTC GGATGTGTAT 
GCATTTGGGA TTGTTTTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAATATCAAC 
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TATCTCCAGA TCTCAGTAAG 
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
AGAGATGAGA GAAAACTCTT TCCTCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
GGGAGCAATA CCAGCCTCTT TCTACCTCAT TTACTCCGTT TGGATTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_027770523.1 ) cDNA ORF clone, Tupaia chinensis(Chinese tree shrew) -> XP_027626324.1 Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTu63109
Clone ID Related Accession (Same CDS sequence)	XM_027770523.1
Accession Version	XM_027770523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Tupaia chinensis(Chinese tree shrew)
Product	serine/threonine-protein kinase B-raf isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006159610.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Tupaia chinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAAGT TGACACAGGA ACATATAGAG GCCCTATTGG ACAAATTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAGCT AGATGCCCTC 
CAACAAAGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACAGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATGCGTCAC GGAGTAACCC CAAGTCACCA 
CAAAAACCTA TTGTTAGAGT CTTCCTGCCC AACAAACAGA GGACAGTGGT ACCTGCAAGG 
TGTGGAGTTA CAGTTCGAGA CAGTCTAAAG AAAGCACTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTGTACAG AATTCAGGAT GGAGAGAAGA AACCAATTGG CTGGGACACT 
GATATTTCTT GGCTTACTGG AGAAGAATTA CATGTGGAAG TGTTGGAAAA TGTACCACTT 
ACAACACATA ACTTTGTACG GAAAACTTTT TTCACCTTAG CGTTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT CCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAAGAGACCT CCTTGGCAGA GACTGCCCTT 
ACATCTGGAG CATCCCCTTC TGCACCTCCC TCAGACTCTC TTGGGCCCCA AATTCTTACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATC CCACAGCCCT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AATTTGGGCA ACGAGACCGA TCCTCATCAG CTCCCAACGT GCATATAAAT 
ACAATAGAAC CTGTCAATAT TGAAGAAAAA TTCCCAGAAG TGGAATTACA GGATCAAAGG 
GACTTGATTA GAGACCAAGG GTTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
ACGCCCCCTG CCTCATTACC TGGCTCACTC ACTAATGTGA AAGCCTTACA GAAATCTCCA 
GGACCTCAGA GAGAAAGGAA GTCATCTTCA TCCTCCGAAG ACAGGAATCG AATGAAAACA 
CTTGGTAGAC GAGACTCAAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
CAAAGAATTG GATCTGGGTC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
GCAGTGAAAA TGTTGAATGT GACAGCACCC ACACCTCAGC AGTTACAGGC CTTCAAAAAT 
GAAGTAGGAG TACTCAGGAA AACACGACAT GTAAATATCC TACTTTTCAT GGGCTATTCC 
ACAAAGCCAC AGCTGGCCAT CGTTACCCAG TGGTGTGAAG GCTCCAGCTT ATATCACCAT 
CTCCACATCA TTGAGACCAA ATTTGAGATG ATAAAGCTTA TAGATATTGC ACGGCAGACT 
GCACAGGGCA TGGATTATTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ACTTTGGTTT AGCCACAGTG 
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGTTCCAT TTTGTGGATG 
GCACCAGAAG TGATCAGAAT GCAAGATAAA AATCCATATA GCTTTCAGTC GGATGTGTAT 
GCATTTGGGA TTGTTTTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAATATCAAC 
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TATCTCCAGA TCTCAGTAAG 
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
AGAGATGAGA GAAAACTCTT TCCTCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
GGAGAATTTG CAGCCTTCAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027626324.1
CDS	36..2219
Translation	MIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASTDTVTSSSSSSLSVLPSSLSVFQNPTDASRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEETSLAETALTSGASPSAPPSDSLGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIEEKFPEVELQDQRDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERKLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK

Target ORF information:

RefSeq Version	XM_027770523.1
Organism	Tupaia chinensis(Chinese tree shrew)
Definition	Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027770523.1

ORF Insert Sequence:

ATGATAAAGT TGACACAGGA ACATATAGAG GCCCTATTGG ACAAATTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAGCT AGATGCCCTC 
CAACAAAGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACAGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATGCGTCAC GGAGTAACCC CAAGTCACCA 
CAAAAACCTA TTGTTAGAGT CTTCCTGCCC AACAAACAGA GGACAGTGGT ACCTGCAAGG 
TGTGGAGTTA CAGTTCGAGA CAGTCTAAAG AAAGCACTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTGTACAG AATTCAGGAT GGAGAGAAGA AACCAATTGG CTGGGACACT 
GATATTTCTT GGCTTACTGG AGAAGAATTA CATGTGGAAG TGTTGGAAAA TGTACCACTT 
ACAACACATA ACTTTGTACG GAAAACTTTT TTCACCTTAG CGTTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT CCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAAGAGACCT CCTTGGCAGA GACTGCCCTT 
ACATCTGGAG CATCCCCTTC TGCACCTCCC TCAGACTCTC TTGGGCCCCA AATTCTTACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATC CCACAGCCCT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AATTTGGGCA ACGAGACCGA TCCTCATCAG CTCCCAACGT GCATATAAAT 
ACAATAGAAC CTGTCAATAT TGAAGAAAAA TTCCCAGAAG TGGAATTACA GGATCAAAGG 
GACTTGATTA GAGACCAAGG GTTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
ACGCCCCCTG CCTCATTACC TGGCTCACTC ACTAATGTGA AAGCCTTACA GAAATCTCCA 
GGACCTCAGA GAGAAAGGAA GTCATCTTCA TCCTCCGAAG ACAGGAATCG AATGAAAACA 
CTTGGTAGAC GAGACTCAAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
CAAAGAATTG GATCTGGGTC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
GCAGTGAAAA TGTTGAATGT GACAGCACCC ACACCTCAGC AGTTACAGGC CTTCAAAAAT 
GAAGTAGGAG TACTCAGGAA AACACGACAT GTAAATATCC TACTTTTCAT GGGCTATTCC 
ACAAAGCCAC AGCTGGCCAT CGTTACCCAG TGGTGTGAAG GCTCCAGCTT ATATCACCAT 
CTCCACATCA TTGAGACCAA ATTTGAGATG ATAAAGCTTA TAGATATTGC ACGGCAGACT 
GCACAGGGCA TGGATTATTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ACTTTGGTTT AGCCACAGTG 
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGTTCCAT TTTGTGGATG 
GCACCAGAAG TGATCAGAAT GCAAGATAAA AATCCATATA GCTTTCAGTC GGATGTGTAT 
GCATTTGGGA TTGTTTTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAATATCAAC 
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TATCTCCAGA TCTCAGTAAG 
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
AGAGATGAGA GAAAACTCTT TCCTCAAATT CTCGCCTCTA TTGAGCTGCT GGCCCGCTCA 
TTGCCAAAAA TTCACCGCAG TGCATCAGAA CCCTCCTTGA ATCGGGCTGG TTTCCAAACA 
GAGGATTTTA GTCTATATGC TTGTGCTTCT CCAAAAACAC CCATCCAGGC AGGGGGATAT 
GGAGAATTTG CAGCCTTCAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_027770524.1 ) cDNA ORF clone, Tupaia chinensis(Chinese tree shrew) -> XP_027626325.1 Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTu63110
Clone ID Related Accession (Same CDS sequence)	XM_027770524.1
Accession Version	XM_027770524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Tupaia chinensis(Chinese tree shrew)
Product	serine/threonine-protein kinase B-raf isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006159610.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Tupaia chinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAAGT TGACACAGGA ACATATAGAG GCCCTATTGG ACAAATTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAGCT AGATGCCCTC 
CAACAAAGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACAGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATGCGTCAC GGAGTAACCC CAAGTCACCA 
CAAAAACCTA TTGTTAGAGT CTTCCTGCCC AACAAACAGA GGACAGTGGT ACCTGCAAGG 
TGTGGAGTTA CAGTTCGAGA CAGTCTAAAG AAAGCACTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTGTACAG AATTCAGGAT GGAGAGAAGA AACCAATTGG CTGGGACACT 
GATATTTCTT GGCTTACTGG AGAAGAATTA CATGTGGAAG TGTTGGAAAA TGTACCACTT 
ACAACACATA ACTTTGTACG GAAAACTTTT TTCACCTTAG CGTTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT CCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAAGAGACCT CCTTGGCAGA GACTGCCCTT 
ACATCTGGAG CATCCCCTTC TGCACCTCCC TCAGACTCTC TTGGGCCCCA AATTCTTACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATC CCACAGCCCT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AATTTGGGCA ACGAGACCGA TCCTCATCAG CTCCCAACGT GCATATAAAT 
ACAATAGAAC CTGTCAATAT TGAAGACTTG ATTAGAGACC AAGGGTTTCG TGGTGATGGA 
GGATCAACCA CAGGTTTGTC TGCTACGCCC CCTGCCTCAT TACCTGGCTC ACTCACTAAT 
GTGAAAGCCT TACAGAAATC TCCAGGACCT CAGAGAGAAA GGAAGTCATC TTCATCCTCC 
GAAGACAGGA ATCGAATGAA AACACTTGGT AGACGAGACT CAAGTGATGA TTGGGAGATT 
CCTGATGGGC AGATTACAGT GGGACAAAGA ATTGGATCTG GGTCATTTGG AACAGTCTAC 
AAGGGAAAGT GGCATGGTGA TGTGGCAGTG AAAATGTTGA ATGTGACAGC ACCCACACCT 
CAGCAGTTAC AGGCCTTCAA AAATGAAGTA GGAGTACTCA GGAAAACACG ACATGTAAAT 
ATCCTACTTT TCATGGGCTA TTCCACAAAG CCACAGCTGG CCATCGTTAC CCAGTGGTGT 
GAAGGCTCCA GCTTATATCA CCATCTCCAC ATCATTGAGA CCAAATTTGA GATGATAAAG 
CTTATAGATA TTGCACGGCA GACTGCACAG GGCATGGATT ATTTACACGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATA TTTCTTCATG AAGACCTCAC AGTAAAAATA 
GGTGACTTTG GTTTAGCCAC AGTGAAATCT CGATGGAGTG GGTCCCATCA GTTTGAACAG 
TTGTCTGGTT CCATTTTGTG GATGGCACCA GAAGTGATCA GAATGCAAGA TAAAAATCCA 
TATAGCTTTC AGTCGGATGT GTATGCATTT GGGATTGTTT TGTATGAATT GATGACTGGA 
CAGTTACCTT ATTCAAATAT CAACAACAGG GACCAGATAA TTTTTATGGT GGGACGAGGA 
TACCTATCTC CAGATCTCAG TAAGGTACGG AGTAACTGTC CAAAAGCCAT GAAGAGATTA 
ATGGCAGAGT GCCTCAAAAA GAAAAGAGAT GAGAGAAAAC TCTTTCCTCA AATTCTCGCC 
TCTATTGAGC TGCTGGCCCG CTCATTGCCA AAAATTCACC GCAGTGCATC AGAACCCTCC 
TTGAATCGGG CTGGTTTCCA AACAGAGGAT TTTAGTCTAT ATGCTTGTGC TTCTCCAAAA 
ACACCCATCC AGGCAGGGGG ATATGGGAGC AATACCAGCC TCTTTCTACC TCATTTACTC 
CGTTTGGATT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027626325.1
CDS	19..2193
Translation	MIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASTDTVTSSSSSSLSVLPSSLSVFQNPTDASRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEETSLAETALTSGASPSAPPSDSLGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIEDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERKLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGSNTSLFLPHLLRLDL

Target ORF information:

RefSeq Version	XM_027770524.1
Organism	Tupaia chinensis(Chinese tree shrew)
Definition	Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027770524.1

ORF Insert Sequence:

ATGATAAAGT TGACACAGGA ACATATAGAG GCCCTATTGG ACAAATTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAGCT AGATGCCCTC 
CAACAAAGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACAGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATGCGTCAC GGAGTAACCC CAAGTCACCA 
CAAAAACCTA TTGTTAGAGT CTTCCTGCCC AACAAACAGA GGACAGTGGT ACCTGCAAGG 
TGTGGAGTTA CAGTTCGAGA CAGTCTAAAG AAAGCACTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTGTACAG AATTCAGGAT GGAGAGAAGA AACCAATTGG CTGGGACACT 
GATATTTCTT GGCTTACTGG AGAAGAATTA CATGTGGAAG TGTTGGAAAA TGTACCACTT 
ACAACACATA ACTTTGTACG GAAAACTTTT TTCACCTTAG CGTTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT CCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAAGAGACCT CCTTGGCAGA GACTGCCCTT 
ACATCTGGAG CATCCCCTTC TGCACCTCCC TCAGACTCTC TTGGGCCCCA AATTCTTACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATC CCACAGCCCT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AATTTGGGCA ACGAGACCGA TCCTCATCAG CTCCCAACGT GCATATAAAT 
ACAATAGAAC CTGTCAATAT TGAAGACTTG ATTAGAGACC AAGGGTTTCG TGGTGATGGA 
GGATCAACCA CAGGTTTGTC TGCTACGCCC CCTGCCTCAT TACCTGGCTC ACTCACTAAT 
GTGAAAGCCT TACAGAAATC TCCAGGACCT CAGAGAGAAA GGAAGTCATC TTCATCCTCC 
GAAGACAGGA ATCGAATGAA AACACTTGGT AGACGAGACT CAAGTGATGA TTGGGAGATT 
CCTGATGGGC AGATTACAGT GGGACAAAGA ATTGGATCTG GGTCATTTGG AACAGTCTAC 
AAGGGAAAGT GGCATGGTGA TGTGGCAGTG AAAATGTTGA ATGTGACAGC ACCCACACCT 
CAGCAGTTAC AGGCCTTCAA AAATGAAGTA GGAGTACTCA GGAAAACACG ACATGTAAAT 
ATCCTACTTT TCATGGGCTA TTCCACAAAG CCACAGCTGG CCATCGTTAC CCAGTGGTGT 
GAAGGCTCCA GCTTATATCA CCATCTCCAC ATCATTGAGA CCAAATTTGA GATGATAAAG 
CTTATAGATA TTGCACGGCA GACTGCACAG GGCATGGATT ATTTACACGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATA TTTCTTCATG AAGACCTCAC AGTAAAAATA 
GGTGACTTTG GTTTAGCCAC AGTGAAATCT CGATGGAGTG GGTCCCATCA GTTTGAACAG 
TTGTCTGGTT CCATTTTGTG GATGGCACCA GAAGTGATCA GAATGCAAGA TAAAAATCCA 
TATAGCTTTC AGTCGGATGT GTATGCATTT GGGATTGTTT TGTATGAATT GATGACTGGA 
CAGTTACCTT ATTCAAATAT CAACAACAGG GACCAGATAA TTTTTATGGT GGGACGAGGA 
TACCTATCTC CAGATCTCAG TAAGGTACGG AGTAACTGTC CAAAAGCCAT GAAGAGATTA 
ATGGCAGAGT GCCTCAAAAA GAAAAGAGAT GAGAGAAAAC TCTTTCCTCA AATTCTCGCC 
TCTATTGAGC TGCTGGCCCG CTCATTGCCA AAAATTCACC GCAGTGCATC AGAACCCTCC 
TTGAATCGGG CTGGTTTCCA AACAGAGGAT TTTAGTCTAT ATGCTTGTGC TTCTCCAAAA 
ACACCCATCC AGGCAGGGGG ATATGGGAGC AATACCAGCC TCTTTCTACC TCATTTACTC 
CGTTTGGATT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_027770525.1 ) cDNA ORF clone, Tupaia chinensis(Chinese tree shrew) -> XP_027626326.1 Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTu63111
Clone ID Related Accession (Same CDS sequence)	XM_027770525.1
Accession Version	XM_027770525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Tupaia chinensis(Chinese tree shrew)
Product	serine/threonine-protein kinase B-raf isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006159610.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Tupaia chinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAAGT TGACACAGGA ACATATAGAG GCCCTATTGG ACAAATTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAGCT AGATGCCCTC 
CAACAAAGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACAGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATGCGTCAC GGAGTAACCC CAAGTCACCA 
CAAAAACCTA TTGTTAGAGT CTTCCTGCCC AACAAACAGA GGACAGTGGT ACCTGCAAGG 
TGTGGAGTTA CAGTTCGAGA CAGTCTAAAG AAAGCACTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTGTACAG AATTCAGGAT GGAGAGAAGA AACCAATTGG CTGGGACACT 
GATATTTCTT GGCTTACTGG AGAAGAATTA CATGTGGAAG TGTTGGAAAA TGTACCACTT 
ACAACACATA ACTTTGTACG GAAAACTTTT TTCACCTTAG CGTTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT CCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAAGAGACCT CCTTGGCAGA GACTGCCCTT 
ACATCTGGAG CATCCCCTTC TGCACCTCCC TCAGACTCTC TTGGGCCCCA AATTCTTACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATC CCACAGCCCT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AATTTGGGCA ACGAGACCGA TCCTCATCAG CTCCCAACGT GCATATAAAT 
ACAATAGAAC CTGTCAATAT TGAAGAAAAA TTCCCAGAAG TGGAATTACA GGATCAAAGG 
GACTTGATTA GAGACCAAGG GTTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
ACGCCCCCTG CCTCATTACC TGGCTCACTC ACTAATGTGA AAGCCTTACA GAAATCTCCA 
GGACCTCAGA GAGAAAGGAA GTCATCTTCA TCCTCCGAAG ACAGGAATCG AATGAAAACA 
CTTGGTAGAC GAGACTCAAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
CAAAGAATTG GATCTGGGTC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
GCAGTGAAAA TGTTGAATGT GACAGCACCC ACACCTCAGC AGTTACAGGC CTTCAAAAAT 
GAAGTAGGAG TACTCAGGAA AACACGACAT GTAAATATCC TACTTTTCAT GGGCTATTCC 
ACAAAGCCAC AGCTGGCCAT CGTTACCCAG TGGTGTGAAG GCTCCAGCTT ATATCACCAT 
CTCCACATCA TTGAGACCAA ATTTGAGATG ATAAAGCTTA TAGATATTGC ACGGCAGACT 
GCACAGGGCA TGGATTATTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ACTTTGGTTT AGCCACAGTG 
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGTTCCAT TTTGTGGATG 
GCACCAGAAG TGATCAGAAT GCAAGATAAA AATCCATATA GCTTTCAGTC GGATGTGTAT 
GCATTTGGGA TTGTTTTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAATATCAAC 
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TATCTCCAGA TCTCAGTAAG 
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
AGAGATGAGA GAAAACTCTT TCCTCAAGAG AATTTGCAGC CTTCAAGTAG CCACACCATC 
ATGGCAGCAT CTACTCTTAT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027626326.1
CDS	36..2102
Translation	MIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASTDTVTSSSSSSLSVLPSSLSVFQNPTDASRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEETSLAETALTSGASPSAPPSDSLGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIEEKFPEVELQDQRDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERKLFPQENLQPSSSHTIMAASTLIS

Target ORF information:

RefSeq Version	XM_027770525.1
Organism	Tupaia chinensis(Chinese tree shrew)
Definition	Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027770525.1

ORF Insert Sequence:

ATGATAAAGT TGACACAGGA ACATATAGAG GCCCTATTGG ACAAATTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAGCT AGATGCCCTC 
CAACAAAGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACAGACAC CGTTACATCT TCTTCCTCTT CTAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATGCGTCAC GGAGTAACCC CAAGTCACCA 
CAAAAACCTA TTGTTAGAGT CTTCCTGCCC AACAAACAGA GGACAGTGGT ACCTGCAAGG 
TGTGGAGTTA CAGTTCGAGA CAGTCTAAAG AAAGCACTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTGTACAG AATTCAGGAT GGAGAGAAGA AACCAATTGG CTGGGACACT 
GATATTTCTT GGCTTACTGG AGAAGAATTA CATGTGGAAG TGTTGGAAAA TGTACCACTT 
ACAACACATA ACTTTGTACG GAAAACTTTT TTCACCTTAG CGTTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT CCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGTTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAAGAGACCT CCTTGGCAGA GACTGCCCTT 
ACATCTGGAG CATCCCCTTC TGCACCTCCC TCAGACTCTC TTGGGCCCCA AATTCTTACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATC CCACAGCCCT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AATTTGGGCA ACGAGACCGA TCCTCATCAG CTCCCAACGT GCATATAAAT 
ACAATAGAAC CTGTCAATAT TGAAGAAAAA TTCCCAGAAG TGGAATTACA GGATCAAAGG 
GACTTGATTA GAGACCAAGG GTTTCGTGGT GATGGAGGAT CAACCACAGG TTTGTCTGCT 
ACGCCCCCTG CCTCATTACC TGGCTCACTC ACTAATGTGA AAGCCTTACA GAAATCTCCA 
GGACCTCAGA GAGAAAGGAA GTCATCTTCA TCCTCCGAAG ACAGGAATCG AATGAAAACA 
CTTGGTAGAC GAGACTCAAG TGATGATTGG GAGATTCCTG ATGGGCAGAT TACAGTGGGA 
CAAAGAATTG GATCTGGGTC ATTTGGAACA GTCTACAAGG GAAAGTGGCA TGGTGATGTG 
GCAGTGAAAA TGTTGAATGT GACAGCACCC ACACCTCAGC AGTTACAGGC CTTCAAAAAT 
GAAGTAGGAG TACTCAGGAA AACACGACAT GTAAATATCC TACTTTTCAT GGGCTATTCC 
ACAAAGCCAC AGCTGGCCAT CGTTACCCAG TGGTGTGAAG GCTCCAGCTT ATATCACCAT 
CTCCACATCA TTGAGACCAA ATTTGAGATG ATAAAGCTTA TAGATATTGC ACGGCAGACT 
GCACAGGGCA TGGATTATTT ACACGCCAAG TCAATCATCC ACAGAGACCT CAAGAGTAAT 
AATATATTTC TTCATGAAGA CCTCACAGTA AAAATAGGTG ACTTTGGTTT AGCCACAGTG 
AAATCTCGAT GGAGTGGGTC CCATCAGTTT GAACAGTTGT CTGGTTCCAT TTTGTGGATG 
GCACCAGAAG TGATCAGAAT GCAAGATAAA AATCCATATA GCTTTCAGTC GGATGTGTAT 
GCATTTGGGA TTGTTTTGTA TGAATTGATG ACTGGACAGT TACCTTATTC AAATATCAAC 
AACAGGGACC AGATAATTTT TATGGTGGGA CGAGGATACC TATCTCCAGA TCTCAGTAAG 
GTACGGAGTA ACTGTCCAAA AGCCATGAAG AGATTAATGG CAGAGTGCCT CAAAAAGAAA 
AGAGATGAGA GAAAACTCTT TCCTCAAGAG AATTTGCAGC CTTCAAGTAG CCACACCATC 
ATGGCAGCAT CTACTCTTAT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRAF ( XM_027770526.1 ) cDNA ORF clone, Tupaia chinensis(Chinese tree shrew) -> XP_027626327.1 Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X6, mRNA.

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CloneID	OTu63112
Clone ID Related Accession (Same CDS sequence)	XM_027770526.1
Accession Version	XM_027770526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Tupaia chinensis(Chinese tree shrew)
Product	serine/threonine-protein kinase B-raf isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006159610.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Tupaia chinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGAG GTCTAATCCC AGAGTGCTGT GCTGTGTACA GAATTCAGGA TGGAGAGAAG 
AAACCAATTG GCTGGGACAC TGATATTTCT TGGCTTACTG GAGAAGAATT ACATGTGGAA 
GTGTTGGAAA ATGTACCACT TACAACACAT AACTTTGTAC GGAAAACTTT TTTCACCTTA 
GCGTTTTGTG ACTTTTGTCG AAAGCTGCTT TTCCAGGGTT TCCGCTGTCA AACATGTGGT 
TATAAATTTC ACCAGCGTTG TAGTACAGAG GTTCCACTGA TGTGTGTTAA TTATGACCAA 
CTTGATTTGC TGTTTGTCTC CAAGTTCTTT GAACACCACC CAATACCACA GGAAGAGACC 
TCCTTGGCAG AGACTGCCCT TACATCTGGA GCATCCCCTT CTGCACCTCC CTCAGACTCT 
CTTGGGCCCC AAATTCTTAC CAGTCCGTCT CCTTCAAAAT CCATTCCAAT CCCACAGCCC 
TTCCGACCAG CAGATGAAGA TCATCGAAAT CAATTTGGGC AACGAGACCG ATCCTCATCA 
GCTCCCAACG TGCATATAAA TACAATAGAA CCTGTCAATA TTGAAGAAAA ATTCCCAGAA 
GTGGAATTAC AGGATCAAAG GGACTTGATT AGAGACCAAG GGTTTCGTGG TGATGGAGGA 
TCAACCACAG GTTTGTCTGC TACGCCCCCT GCCTCATTAC CTGGCTCACT CACTAATGTG 
AAAGCCTTAC AGAAATCTCC AGGACCTCAG AGAGAAAGGA AGTCATCTTC ATCCTCCGAA 
GACAGGAATC GAATGAAAAC ACTTGGTAGA CGAGACTCAA GTGATGATTG GGAGATTCCT 
GATGGGCAGA TTACAGTGGG ACAAAGAATT GGATCTGGGT CATTTGGAAC AGTCTACAAG 
GGAAAGTGGC ATGGTGATGT GGCAGTGAAA ATGTTGAATG TGACAGCACC CACACCTCAG 
CAGTTACAGG CCTTCAAAAA TGAAGTAGGA GTACTCAGGA AAACACGACA TGTAAATATC 
CTACTTTTCA TGGGCTATTC CACAAAGCCA CAGCTGGCCA TCGTTACCCA GTGGTGTGAA 
GGCTCCAGCT TATATCACCA TCTCCACATC ATTGAGACCA AATTTGAGAT GATAAAGCTT 
ATAGATATTG CACGGCAGAC TGCACAGGGC ATGGATTATT TACACGCCAA GTCAATCATC 
CACAGAGACC TCAAGAGTAA TAATATATTT CTTCATGAAG ACCTCACAGT AAAAATAGGT 
GACTTTGGTT TAGCCACAGT GAAATCTCGA TGGAGTGGGT CCCATCAGTT TGAACAGTTG 
TCTGGTTCCA TTTTGTGGAT GGCACCAGAA GTGATCAGAA TGCAAGATAA AAATCCATAT 
AGCTTTCAGT CGGATGTGTA TGCATTTGGG ATTGTTTTGT ATGAATTGAT GACTGGACAG 
TTACCTTATT CAAATATCAA CAACAGGGAC CAGATAATTT TTATGGTGGG ACGAGGATAC 
CTATCTCCAG ATCTCAGTAA GGTACGGAGT AACTGTCCAA AAGCCATGAA GAGATTAATG 
GCAGAGTGCC TCAAAAAGAA AAGAGATGAG AGAAAACTCT TTCCTCAAAT TCTCGCCTCT 
ATTGAGCTGC TGGCCCGCTC ATTGCCAAAA ATTCACCGCA GTGCATCAGA ACCCTCCTTG 
AATCGGGCTG GTTTCCAAAC AGAGGATTTT AGTCTATATG CTTGTGCTTC TCCAAAAACA 
CCCATCCAGG CAGGGGGATA TGGGAGCAAT ACCAGCCTCT TTCTACCTCA TTTACTCCGT 
TTGGATTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027626327.1
CDS	71..1882
Translation	MMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEETSLAETALTSGASPSAPPSDSLGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIEEKFPEVELQDQRDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERKLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGSNTSLFLPHLLRLDL

Target ORF information:

RefSeq Version	XM_027770526.1
Organism	Tupaia chinensis(Chinese tree shrew)
Definition	Tupaia chinensis B-Raf proto-oncogene, serine/threonine kinase (BRAF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027770526.1

ORF Insert Sequence:

ATGATGAGAG GTCTAATCCC AGAGTGCTGT GCTGTGTACA GAATTCAGGA TGGAGAGAAG 
AAACCAATTG GCTGGGACAC TGATATTTCT TGGCTTACTG GAGAAGAATT ACATGTGGAA 
GTGTTGGAAA ATGTACCACT TACAACACAT AACTTTGTAC GGAAAACTTT TTTCACCTTA 
GCGTTTTGTG ACTTTTGTCG AAAGCTGCTT TTCCAGGGTT TCCGCTGTCA AACATGTGGT 
TATAAATTTC ACCAGCGTTG TAGTACAGAG GTTCCACTGA TGTGTGTTAA TTATGACCAA 
CTTGATTTGC TGTTTGTCTC CAAGTTCTTT GAACACCACC CAATACCACA GGAAGAGACC 
TCCTTGGCAG AGACTGCCCT TACATCTGGA GCATCCCCTT CTGCACCTCC CTCAGACTCT 
CTTGGGCCCC AAATTCTTAC CAGTCCGTCT CCTTCAAAAT CCATTCCAAT CCCACAGCCC 
TTCCGACCAG CAGATGAAGA TCATCGAAAT CAATTTGGGC AACGAGACCG ATCCTCATCA 
GCTCCCAACG TGCATATAAA TACAATAGAA CCTGTCAATA TTGAAGAAAA ATTCCCAGAA 
GTGGAATTAC AGGATCAAAG GGACTTGATT AGAGACCAAG GGTTTCGTGG TGATGGAGGA 
TCAACCACAG GTTTGTCTGC TACGCCCCCT GCCTCATTAC CTGGCTCACT CACTAATGTG 
AAAGCCTTAC AGAAATCTCC AGGACCTCAG AGAGAAAGGA AGTCATCTTC ATCCTCCGAA 
GACAGGAATC GAATGAAAAC ACTTGGTAGA CGAGACTCAA GTGATGATTG GGAGATTCCT 
GATGGGCAGA TTACAGTGGG ACAAAGAATT GGATCTGGGT CATTTGGAAC AGTCTACAAG 
GGAAAGTGGC ATGGTGATGT GGCAGTGAAA ATGTTGAATG TGACAGCACC CACACCTCAG 
CAGTTACAGG CCTTCAAAAA TGAAGTAGGA GTACTCAGGA AAACACGACA TGTAAATATC 
CTACTTTTCA TGGGCTATTC CACAAAGCCA CAGCTGGCCA TCGTTACCCA GTGGTGTGAA 
GGCTCCAGCT TATATCACCA TCTCCACATC ATTGAGACCA AATTTGAGAT GATAAAGCTT 
ATAGATATTG CACGGCAGAC TGCACAGGGC ATGGATTATT TACACGCCAA GTCAATCATC 
CACAGAGACC TCAAGAGTAA TAATATATTT CTTCATGAAG ACCTCACAGT AAAAATAGGT 
GACTTTGGTT TAGCCACAGT GAAATCTCGA TGGAGTGGGT CCCATCAGTT TGAACAGTTG 
TCTGGTTCCA TTTTGTGGAT GGCACCAGAA GTGATCAGAA TGCAAGATAA AAATCCATAT 
AGCTTTCAGT CGGATGTGTA TGCATTTGGG ATTGTTTTGT ATGAATTGAT GACTGGACAG 
TTACCTTATT CAAATATCAA CAACAGGGAC CAGATAATTT TTATGGTGGG ACGAGGATAC 
CTATCTCCAG ATCTCAGTAA GGTACGGAGT AACTGTCCAA AAGCCATGAA GAGATTAATG 
GCAGAGTGCC TCAAAAAGAA AAGAGATGAG AGAAAACTCT TTCCTCAAAT TCTCGCCTCT 
ATTGAGCTGC TGGCCCGCTC ATTGCCAAAA ATTCACCGCA GTGCATCAGA ACCCTCCTTG 
AATCGGGCTG GTTTCCAAAC AGAGGATTTT AGTCTATATG CTTGTGCTTC TCCAAAAACA 
CCCATCCAGG CAGGGGGATA TGGGAGCAAT ACCAGCCTCT TTCTACCTCA TTTACTCCGT 
TTGGATTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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BRAF cDNA ORF clone, Tupaia chinensis(Chinese tree shrew)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム