LOC101360377 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101360377
Entrez Gene ID	101360377
Full Name	DEAH-box helicase 35
Synonyms	DHX35
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004370346.3	XP_004370403.1	probable ATP-dependent RNA helicase DHX35 isoform X2
XM_023724686.1	XP_023580454.1	probable ATP-dependent RNA helicase DHX35 isoform X1
XM_004370346.2	XP_004370403.1	probable ATP-dependent RNA helicase DHX35

Pathways from BioSystems
Gene Ontology

The following LOC101360377 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101360377 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTr166489	XM_004370346.3 Latest version!	Trichechus manatus latirostris DEAH-box helicase 35 (LOC101360377), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OTr215300	XM_023724686.1 Latest version!	Trichechus manatus latirostris DEAH-box helicase 35 (LOC101360377), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OTr166489	XM_004370346.2	Trichechus manatus latirostris DEAH (Asp-Glu-Ala-His) box polypeptide 35 (LOC101360377), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101360377 ( XM_004370346.3 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004370403.1 Trichechus manatus latirostris DEAH-box helicase 35 (LOC101360377), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTr166489
Clone ID Related Accession (Same CDS sequence)	XM_004370346.3 , XM_004370346.2
Accession Version	XM_004370346.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	probable ATP-dependent RNA helicase DHX35 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443944.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 25, 2018 this sequence version replaced XM_004370346.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGC CCGTGGGACC AGTGAAGTTC TGGCGACCCG GTACGGAGGG GCCAGGTGTC 
AGCATCTCTG AAGAGAGACA AAGTCTAGCG GAAAACTCTG TGACAACTGT TGTTTACAAT 
GCTTACGCTG CCCTTTCTAT AGAGCAACAG AGGCAGAAGC TGCCGGTTTT CAAGCTTAGG 
AATCATATAT TATACTTGAT AGAAAACTGT CAGACGGTGG TGATTGTTGG AGAAACGGGA 
TGTGGAAAAA GCACACAGAT CCCACAATAC CTTGCAGAAG CTGGCTGGAC AGCCGAAGGA 
AGAGTTGTGG GCGTGACCCA GCCTCGAAGA GTGGCTGCCG TGACAGTTGC AGGGAGAGTA 
GCTGAGGAAA GAGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGACGAC 
TGCACTGACC CGCTGGCTAC AAGAATTAAG TTTCTGACCG ATGGAATGCT GGTCAGGGAA 
ATGATGGTTG ACCCTTTGTT AACAAAATAT AGTGCCATCA TGCTGGATGA AGCTCATGAG 
AGGACCTTGT ACACCGACAT TGCCATTGGC TTGTTAAAAA AGATTCAGAA AAAGCGAGGG 
GATCTCCGCT TGATTGTGGC CTCAGCCACT CTGGATGCAG AGAAATTTCG AGATTTCTTT 
AATCAAAATG AAACCAGTGA CCCGACCAGA GATACGTGTG TGATCCTTAC AGTGGAAGGG 
CGAACGTTTC CAGTGGATAT CTTTTATCTG CAAAGTCCTG TTCCAGATTA TATCAAATCA 
ACTGTGGAAA CTGTGATGAA AATTCACCAG ACAGAGGGCG ATGGAGACAT ATTAGCATTT 
CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCTATGC TAATCGAGCA CGCTCGAGAG 
CTGGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATTC AGGACTGCCT 
TCCTTTGAGC AAATGAAAGT GTTTGAACGA GTGCCACGCA GTGTCAGGAA GGTGATAGTG 
GCCACCAATG TGGCAGAAAC CTCCATCACC ATCAGCGGGA TTGTATATGT GATTGACTGC 
GGCTTCATGA AACTCCGTGC CTACAATCCC AGAACAGCTA TTGAATGCTT GGTGGTGGTC 
CCGGTGTCCC AGGCCTCAGC CAACCAGCGA GCAGGGCGTG GTGGTCGTAA CCGCTCAGGG 
AAATGTTATC GACTTTATAC AGAGGAAGCC TTTGATAAGT TGCCTCAATC CACCGTTCCT 
GAGATGCAGC GCAGCAATTT GGCGCCTGTC ATCCTGCAGC TGAAGGCGCT GGGAATTGAC 
AATGTCCTCA GGTTCCACTT CATGTCGCCT CCTCCAGCAC AGTCAATGGT TCAAGCTTTG 
GAGTTACTCT ATGCTCTGGG AGGTCTGGAC AAAGACTGTC GGCTAACTGA ACCGCTTGGC 
ATGAGGATAG CGGAGTTTCC TTTGAATCCC ATGTTTGCAA AAATGCTGCT TGAATCAGGA 
AATTTTGGCT GTTCTCAGGA AATCCTAAGC ATTGCGGCCA TGATGCAGAT CCAGAATATC 
TTTGTGATCC CCCCAAACCA GAAGTCCCAG GCAATTCGAG TGCACCGAAG ATTTGCTGTG 
GAGGAGGGTG ATCATCTCAC TATGCTCAAC GTGTATGAAG CATTCATCAA ACACAATAAG 
AACTCGCAGT GGTGTCAGGA ACATTTCCTG AATTACAAGG GCCTTGTCAG AGCTGCAACG 
GTGCGAGAAC AATTGAAAAA GCTTCTTGTC AAGTTCCAAG TGCCCAAGAA ATCTAGTGAA 
GGTGATCCGG ATCCGGTTCT GAGGTGCATT GTCTCGGGAT TCTTTGCAAA TGCTGCGAGG 
TTTCATTCTT CTGGAGTTTA TAGGACTACC CGTGATGACC ACGAATTGCA CATCCACCCT 
GCATCAGTCC TCTATGCAGA GAAGCCGCCT CGCTGGGTTG TCTACAATGA AGTTATACAA 
ACCTCCAAGT ATTATATGAG AGATGTGACT GCTATTGAAT CTGCTTGGCT GTTGGAGCTG 
GCTCCACACT TTTATCAACA AGGAACGCAC CTGTCCCTGA AAGCCAAAAG GACCAAGGTC 
CAGGACTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004370403.1
CDS	140..2251
Translation	MAAPVGPVKFWRPGTEGPGVSISEERQSLAENSVTTVVYNAYAALSIEQQRQKLPVFKLRNHILYLIENCQTVVIVGETGCGKSTQIPQYLAEAGWTAEGRVVGVTQPRRVAAVTVAGRVAEERGAVLGHEVGYCIRFDDCTDPLATRIKFLTDGMLVREMMVDPLLTKYSAIMLDEAHERTLYTDIAIGLLKKIQKKRGDLRLIVASATLDAEKFRDFFNQNETSDPTRDTCVILTVEGRTFPVDIFYLQSPVPDYIKSTVETVMKIHQTEGDGDILAFLTGQEEVETVVSMLIEHARELARTGMKRHLRVLPMYSGLPSFEQMKVFERVPRSVRKVIVATNVAETSITISGIVYVIDCGFMKLRAYNPRTAIECLVVVPVSQASANQRAGRGGRNRSGKCYRLYTEEAFDKLPQSTVPEMQRSNLAPVILQLKALGIDNVLRFHFMSPPPAQSMVQALELLYALGGLDKDCRLTEPLGMRIAEFPLNPMFAKMLLESGNFGCSQEILSIAAMMQIQNIFVIPPNQKSQAIRVHRRFAVEEGDHLTMLNVYEAFIKHNKNSQWCQEHFLNYKGLVRAATVREQLKKLLVKFQVPKKSSEGDPDPVLRCIVSGFFANAARFHSSGVYRTTRDDHELHIHPASVLYAEKPPRWVVYNEVIQTSKYYMRDVTAIESAWLLELAPHFYQQGTHLSLKAKRTKVQDL

Target ORF information:

RefSeq Version	XM_004370346.3
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris DEAH-box helicase 35 (LOC101360377), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004370346.3

ORF Insert Sequence:

ATGGCTGCGC CCGTGGGACC AGTGAAGTTC TGGCGACCCG GTACGGAGGG GCCAGGTGTC 
AGCATCTCTG AAGAGAGACA AAGTCTAGCG GAAAACTCTG TGACAACTGT TGTTTACAAT 
GCTTACGCTG CCCTTTCTAT AGAGCAACAG AGGCAGAAGC TGCCGGTTTT CAAGCTTAGG 
AATCATATAT TATACTTGAT AGAAAACTGT CAGACGGTGG TGATTGTTGG AGAAACGGGA 
TGTGGAAAAA GCACACAGAT CCCACAATAC CTTGCAGAAG CTGGCTGGAC AGCCGAAGGA 
AGAGTTGTGG GCGTGACCCA GCCTCGAAGA GTGGCTGCCG TGACAGTTGC AGGGAGAGTA 
GCTGAGGAAA GAGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGACGAC 
TGCACTGACC CGCTGGCTAC AAGAATTAAG TTTCTGACCG ATGGAATGCT GGTCAGGGAA 
ATGATGGTTG ACCCTTTGTT AACAAAATAT AGTGCCATCA TGCTGGATGA AGCTCATGAG 
AGGACCTTGT ACACCGACAT TGCCATTGGC TTGTTAAAAA AGATTCAGAA AAAGCGAGGG 
GATCTCCGCT TGATTGTGGC CTCAGCCACT CTGGATGCAG AGAAATTTCG AGATTTCTTT 
AATCAAAATG AAACCAGTGA CCCGACCAGA GATACGTGTG TGATCCTTAC AGTGGAAGGG 
CGAACGTTTC CAGTGGATAT CTTTTATCTG CAAAGTCCTG TTCCAGATTA TATCAAATCA 
ACTGTGGAAA CTGTGATGAA AATTCACCAG ACAGAGGGCG ATGGAGACAT ATTAGCATTT 
CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCTATGC TAATCGAGCA CGCTCGAGAG 
CTGGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATTC AGGACTGCCT 
TCCTTTGAGC AAATGAAAGT GTTTGAACGA GTGCCACGCA GTGTCAGGAA GGTGATAGTG 
GCCACCAATG TGGCAGAAAC CTCCATCACC ATCAGCGGGA TTGTATATGT GATTGACTGC 
GGCTTCATGA AACTCCGTGC CTACAATCCC AGAACAGCTA TTGAATGCTT GGTGGTGGTC 
CCGGTGTCCC AGGCCTCAGC CAACCAGCGA GCAGGGCGTG GTGGTCGTAA CCGCTCAGGG 
AAATGTTATC GACTTTATAC AGAGGAAGCC TTTGATAAGT TGCCTCAATC CACCGTTCCT 
GAGATGCAGC GCAGCAATTT GGCGCCTGTC ATCCTGCAGC TGAAGGCGCT GGGAATTGAC 
AATGTCCTCA GGTTCCACTT CATGTCGCCT CCTCCAGCAC AGTCAATGGT TCAAGCTTTG 
GAGTTACTCT ATGCTCTGGG AGGTCTGGAC AAAGACTGTC GGCTAACTGA ACCGCTTGGC 
ATGAGGATAG CGGAGTTTCC TTTGAATCCC ATGTTTGCAA AAATGCTGCT TGAATCAGGA 
AATTTTGGCT GTTCTCAGGA AATCCTAAGC ATTGCGGCCA TGATGCAGAT CCAGAATATC 
TTTGTGATCC CCCCAAACCA GAAGTCCCAG GCAATTCGAG TGCACCGAAG ATTTGCTGTG 
GAGGAGGGTG ATCATCTCAC TATGCTCAAC GTGTATGAAG CATTCATCAA ACACAATAAG 
AACTCGCAGT GGTGTCAGGA ACATTTCCTG AATTACAAGG GCCTTGTCAG AGCTGCAACG 
GTGCGAGAAC AATTGAAAAA GCTTCTTGTC AAGTTCCAAG TGCCCAAGAA ATCTAGTGAA 
GGTGATCCGG ATCCGGTTCT GAGGTGCATT GTCTCGGGAT TCTTTGCAAA TGCTGCGAGG 
TTTCATTCTT CTGGAGTTTA TAGGACTACC CGTGATGACC ACGAATTGCA CATCCACCCT 
GCATCAGTCC TCTATGCAGA GAAGCCGCCT CGCTGGGTTG TCTACAATGA AGTTATACAA 
ACCTCCAAGT ATTATATGAG AGATGTGACT GCTATTGAAT CTGCTTGGCT GTTGGAGCTG 
GCTCCACACT TTTATCAACA AGGAACGCAC CTGTCCCTGA AAGCCAAAAG GACCAAGGTC 
CAGGACTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101360377 ( XM_004370346.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004370403.1 Trichechus manatus latirostris DEAH (Asp-Glu-Ala-His) box polypeptide 35 (LOC101360377), mRNA.

Price & Availability↑

CloneID	OTr166489
Clone ID Related Accession (Same CDS sequence)	XM_004370346.3 , XM_004370346.2
Accession Version	XM_004370346.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	probable ATP-dependent RNA helicase DHX35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443944.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2015 this sequence version replaced XM_004370346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGC CCGTGGGACC AGTGAAGTTC TGGCGACCCG GTACGGAGGG GCCAGGTGTC 
AGCATCTCTG AAGAGAGACA AAGTCTAGCG GAAAACTCTG TGACAACTGT TGTTTACAAT 
GCTTACGCTG CCCTTTCTAT AGAGCAACAG AGGCAGAAGC TGCCGGTTTT CAAGCTTAGG 
AATCATATAT TATACTTGAT AGAAAACTGT CAGACGGTGG TGATTGTTGG AGAAACGGGA 
TGTGGAAAAA GCACACAGAT CCCACAATAC CTTGCAGAAG CTGGCTGGAC AGCCGAAGGA 
AGAGTTGTGG GCGTGACCCA GCCTCGAAGA GTGGCTGCCG TGACAGTTGC AGGGAGAGTA 
GCTGAGGAAA GAGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGACGAC 
TGCACTGACC CGCTGGCTAC AAGAATTAAG TTTCTGACCG ATGGAATGCT GGTCAGGGAA 
ATGATGGTTG ACCCTTTGTT AACAAAATAT AGTGCCATCA TGCTGGATGA AGCTCATGAG 
AGGACCTTGT ACACCGACAT TGCCATTGGC TTGTTAAAAA AGATTCAGAA AAAGCGAGGG 
GATCTCCGCT TGATTGTGGC CTCAGCCACT CTGGATGCAG AGAAATTTCG AGATTTCTTT 
AATCAAAATG AAACCAGTGA CCCGACCAGA GATACGTGTG TGATCCTTAC AGTGGAAGGG 
CGAACGTTTC CAGTGGATAT CTTTTATCTG CAAAGTCCTG TTCCAGATTA TATCAAATCA 
ACTGTGGAAA CTGTGATGAA AATTCACCAG ACAGAGGGCG ATGGAGACAT ATTAGCATTT 
CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCTATGC TAATCGAGCA CGCTCGAGAG 
CTGGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATTC AGGACTGCCT 
TCCTTTGAGC AAATGAAAGT GTTTGAACGA GTGCCACGCA GTGTCAGGAA GGTGATAGTG 
GCCACCAATG TGGCAGAAAC CTCCATCACC ATCAGCGGGA TTGTATATGT GATTGACTGC 
GGCTTCATGA AACTCCGTGC CTACAATCCC AGAACAGCTA TTGAATGCTT GGTGGTGGTC 
CCGGTGTCCC AGGCCTCAGC CAACCAGCGA GCAGGGCGTG GTGGTCGTAA CCGCTCAGGG 
AAATGTTATC GACTTTATAC AGAGGAAGCC TTTGATAAGT TGCCTCAATC CACCGTTCCT 
GAGATGCAGC GCAGCAATTT GGCGCCTGTC ATCCTGCAGC TGAAGGCGCT GGGAATTGAC 
AATGTCCTCA GGTTCCACTT CATGTCGCCT CCTCCAGCAC AGTCAATGGT TCAAGCTTTG 
GAGTTACTCT ATGCTCTGGG AGGTCTGGAC AAAGACTGTC GGCTAACTGA ACCGCTTGGC 
ATGAGGATAG CGGAGTTTCC TTTGAATCCC ATGTTTGCAA AAATGCTGCT TGAATCAGGA 
AATTTTGGCT GTTCTCAGGA AATCCTAAGC ATTGCGGCCA TGATGCAGAT CCAGAATATC 
TTTGTGATCC CCCCAAACCA GAAGTCCCAG GCAATTCGAG TGCACCGAAG ATTTGCTGTG 
GAGGAGGGTG ATCATCTCAC TATGCTCAAC GTGTATGAAG CATTCATCAA ACACAATAAG 
AACTCGCAGT GGTGTCAGGA ACATTTCCTG AATTACAAGG GCCTTGTCAG AGCTGCAACG 
GTGCGAGAAC AATTGAAAAA GCTTCTTGTC AAGTTCCAAG TGCCCAAGAA ATCTAGTGAA 
GGTGATCCGG ATCCGGTTCT GAGGTGCATT GTCTCGGGAT TCTTTGCAAA TGCTGCGAGG 
TTTCATTCTT CTGGAGTTTA TAGGACTACC CGTGATGACC ACGAATTGCA CATCCACCCT 
GCATCAGTCC TCTATGCAGA GAAGCCGCCT CGCTGGGTTG TCTACAATGA AGTTATACAA 
ACCTCCAAGT ATTATATGAG AGATGTGACT GCTATTGAAT CTGCTTGGCT GTTGGAGCTG 
GCTCCACACT TTTATCAACA AGGAACGCAC CTGTCCCTGA AAGCCAAAAG GACCAAGGTC 
CAGGACTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004370403.1
CDS	23..2134
Translation	MAAPVGPVKFWRPGTEGPGVSISEERQSLAENSVTTVVYNAYAALSIEQQRQKLPVFKLRNHILYLIENCQTVVIVGETGCGKSTQIPQYLAEAGWTAEGRVVGVTQPRRVAAVTVAGRVAEERGAVLGHEVGYCIRFDDCTDPLATRIKFLTDGMLVREMMVDPLLTKYSAIMLDEAHERTLYTDIAIGLLKKIQKKRGDLRLIVASATLDAEKFRDFFNQNETSDPTRDTCVILTVEGRTFPVDIFYLQSPVPDYIKSTVETVMKIHQTEGDGDILAFLTGQEEVETVVSMLIEHARELARTGMKRHLRVLPMYSGLPSFEQMKVFERVPRSVRKVIVATNVAETSITISGIVYVIDCGFMKLRAYNPRTAIECLVVVPVSQASANQRAGRGGRNRSGKCYRLYTEEAFDKLPQSTVPEMQRSNLAPVILQLKALGIDNVLRFHFMSPPPAQSMVQALELLYALGGLDKDCRLTEPLGMRIAEFPLNPMFAKMLLESGNFGCSQEILSIAAMMQIQNIFVIPPNQKSQAIRVHRRFAVEEGDHLTMLNVYEAFIKHNKNSQWCQEHFLNYKGLVRAATVREQLKKLLVKFQVPKKSSEGDPDPVLRCIVSGFFANAARFHSSGVYRTTRDDHELHIHPASVLYAEKPPRWVVYNEVIQTSKYYMRDVTAIESAWLLELAPHFYQQGTHLSLKAKRTKVQDL

Target ORF information:

RefSeq Version	XM_004370346.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris DEAH (Asp-Glu-Ala-His) box polypeptide 35 (LOC101360377), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004370346.2

ORF Insert Sequence:

ATGGCTGCGC CCGTGGGACC AGTGAAGTTC TGGCGACCCG GTACGGAGGG GCCAGGTGTC 
AGCATCTCTG AAGAGAGACA AAGTCTAGCG GAAAACTCTG TGACAACTGT TGTTTACAAT 
GCTTACGCTG CCCTTTCTAT AGAGCAACAG AGGCAGAAGC TGCCGGTTTT CAAGCTTAGG 
AATCATATAT TATACTTGAT AGAAAACTGT CAGACGGTGG TGATTGTTGG AGAAACGGGA 
TGTGGAAAAA GCACACAGAT CCCACAATAC CTTGCAGAAG CTGGCTGGAC AGCCGAAGGA 
AGAGTTGTGG GCGTGACCCA GCCTCGAAGA GTGGCTGCCG TGACAGTTGC AGGGAGAGTA 
GCTGAGGAAA GAGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGACGAC 
TGCACTGACC CGCTGGCTAC AAGAATTAAG TTTCTGACCG ATGGAATGCT GGTCAGGGAA 
ATGATGGTTG ACCCTTTGTT AACAAAATAT AGTGCCATCA TGCTGGATGA AGCTCATGAG 
AGGACCTTGT ACACCGACAT TGCCATTGGC TTGTTAAAAA AGATTCAGAA AAAGCGAGGG 
GATCTCCGCT TGATTGTGGC CTCAGCCACT CTGGATGCAG AGAAATTTCG AGATTTCTTT 
AATCAAAATG AAACCAGTGA CCCGACCAGA GATACGTGTG TGATCCTTAC AGTGGAAGGG 
CGAACGTTTC CAGTGGATAT CTTTTATCTG CAAAGTCCTG TTCCAGATTA TATCAAATCA 
ACTGTGGAAA CTGTGATGAA AATTCACCAG ACAGAGGGCG ATGGAGACAT ATTAGCATTT 
CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCTATGC TAATCGAGCA CGCTCGAGAG 
CTGGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATTC AGGACTGCCT 
TCCTTTGAGC AAATGAAAGT GTTTGAACGA GTGCCACGCA GTGTCAGGAA GGTGATAGTG 
GCCACCAATG TGGCAGAAAC CTCCATCACC ATCAGCGGGA TTGTATATGT GATTGACTGC 
GGCTTCATGA AACTCCGTGC CTACAATCCC AGAACAGCTA TTGAATGCTT GGTGGTGGTC 
CCGGTGTCCC AGGCCTCAGC CAACCAGCGA GCAGGGCGTG GTGGTCGTAA CCGCTCAGGG 
AAATGTTATC GACTTTATAC AGAGGAAGCC TTTGATAAGT TGCCTCAATC CACCGTTCCT 
GAGATGCAGC GCAGCAATTT GGCGCCTGTC ATCCTGCAGC TGAAGGCGCT GGGAATTGAC 
AATGTCCTCA GGTTCCACTT CATGTCGCCT CCTCCAGCAC AGTCAATGGT TCAAGCTTTG 
GAGTTACTCT ATGCTCTGGG AGGTCTGGAC AAAGACTGTC GGCTAACTGA ACCGCTTGGC 
ATGAGGATAG CGGAGTTTCC TTTGAATCCC ATGTTTGCAA AAATGCTGCT TGAATCAGGA 
AATTTTGGCT GTTCTCAGGA AATCCTAAGC ATTGCGGCCA TGATGCAGAT CCAGAATATC 
TTTGTGATCC CCCCAAACCA GAAGTCCCAG GCAATTCGAG TGCACCGAAG ATTTGCTGTG 
GAGGAGGGTG ATCATCTCAC TATGCTCAAC GTGTATGAAG CATTCATCAA ACACAATAAG 
AACTCGCAGT GGTGTCAGGA ACATTTCCTG AATTACAAGG GCCTTGTCAG AGCTGCAACG 
GTGCGAGAAC AATTGAAAAA GCTTCTTGTC AAGTTCCAAG TGCCCAAGAA ATCTAGTGAA 
GGTGATCCGG ATCCGGTTCT GAGGTGCATT GTCTCGGGAT TCTTTGCAAA TGCTGCGAGG 
TTTCATTCTT CTGGAGTTTA TAGGACTACC CGTGATGACC ACGAATTGCA CATCCACCCT 
GCATCAGTCC TCTATGCAGA GAAGCCGCCT CGCTGGGTTG TCTACAATGA AGTTATACAA 
ACCTCCAAGT ATTATATGAG AGATGTGACT GCTATTGAAT CTGCTTGGCT GTTGGAGCTG 
GCTCCACACT TTTATCAACA AGGAACGCAC CTGTCCCTGA AAGCCAAAAG GACCAAGGTC 
CAGGACTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101360377 ( XM_023724686.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023580454.1 Trichechus manatus latirostris DEAH-box helicase 35 (LOC101360377), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTr215300
Clone ID Related Accession (Same CDS sequence)	XM_023724686.1
Accession Version	XM_023724686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	probable ATP-dependent RNA helicase DHX35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443944.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGC CCGTGGGACC AGTGAAGTTC TGGCGACCCG GTACGGAGGG GCCAGGTGTC 
AGCATCTCTG AAGAGAGACA AAGTCTAGCG GAAAACTCTG TGACAACTGT TGTTTACAAT 
GCTTACGCTG CCCTTTCTAT AGAGCAACAG AGGCAGAAGC TGCCGGTTTT CAAGCTTAGG 
AATCATATAT TATACTTGAT AGAAAACTGT CAGACGGTGG TGATTGTTGG AGAAACGGGA 
TGTGGAAAAA GCACACAGAT CCCACAATAC CTTGCAGAAG CTGGCTGGAC AGCCGAAGGA 
AGAGTTGTGG GCGTGACCCA GCCTCGAAGA GTGGCTGCCG TGACAGTTGC AGGGAGAGTA 
GCTGAGGAAA GAGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGACGAC 
TGCACTGACC CGCTGGCTAC AAGAATTAAG TTTCTGACCG ATGGAATGCT GGTCAGGGAA 
ATGATGGTTG ACCCTTTGTT AACAAAATAT AGTGCCATCA TGCTGGATGA AGCTCATGAG 
AGGACCTTGT ACACCGACAT TGCCATTGGC TTGTTAAAAA AGATTCAGAA AAAGCGAGGG 
GATCTCCGCT TGATTGTGGC CTCAGCCACT CTGGATGCAG AGAAATTTCG AGATTTCTTT 
AATCAAAATG AAACCAGTGA CCCGACCAGA GATACGTGTG TGATCCTTAC AGTGGAAGGG 
CGAACGTTTC CAGTGGATAT CTTTTATCTG CAAAGTCCTG TTCCAGATTA TATCAAATCA 
ACTGTGGAAA CTGTGATGAA AATTCACCAG ACAGAGGGCG ATGGAGACAT ATTAGCATTT 
CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCTATGC TAATCGAGCA CGCTCGAGAG 
CTGGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATTC AGGACTGCCT 
TCCTTTGAGC AAATGAAAGT GTTTGAACGA GTGCCACGCA GTGTCAGGAA GGTGATAGTG 
GCCACCAATG TGGCAGAAAC CTCCATCACC ATCAGCGGGA TTGTATATGT GATTGACTGC 
GGCTTCATGA AACTCCGTGC CTACAATCCC AGAACAGCTA TTGAATGCTT GGTGGTGGTC 
CCGGTGTCCC AGGCCTCAGC CAACCAGCGA GCAGGGCGTG GTGGTCGTAA CCGCTCAGGG 
AAATGTTATC GACTTTATAC AGAGGAAGCC TTTGATAAGT TGCCTCAATC CACCGTTCCT 
GAGATGCAGC GCAGCAATTT GGCGCCTGTC ATCCTGCAGC TGAAGGCGCT GGGAATTGAC 
AATGTCCTCA GGTTCCACTT CATGTCGCCT CCTCCAGCAC AGTCAATGGT TCAAGCTTTG 
GAGTTACTCT ATGCTCTGGG AGGTCTGGAC AAAGACTGTC GGCTAACTGA ACCGCTTGGC 
ATGAGGATAG CGGAGTTTCC TTTGAATCCC ATGTTTGCAA AAATGCTGCT TGAATCAGGA 
AATTTTGGCT GTTCTCAGGA AATCCTAAGC ATTGCGGCCA TGATGCAGAT CCAGAATATC 
TTTGTGATCC CCCCAAACCA GAAGTCCCAG GCAATTCGAG TGCACCGAAG ATTTGCTGTG 
GAGGAGGGTG ATCATCTCAC TATGCTCAAC GTGTATGAAG CATTCATCAA ACACAATAAG 
AACTCGCAGT GGTGTCAGGA ACATTTCCTG AATTACAAGG GCCTTGTCAG AGCTGCAACG 
GTGCGAGAAC AATTGAAAAA GCTTCTTGTC AAGTTCCAAG TGCCCAAGAA ATCTAGTGAA 
GGTGATCCGG ATCCGGTTCT GAGGTGCATT GTCTCGGGAT TCTTTGCAAA TGCTGCGAGG 
TTTCATTCTT CTGGAGTTTA TAGGACTACC CGTGATGACC ACGAATTGCA CATCCACCCT 
GCATCAGTCC TCTATGCAGA GAAGCCGCCT CGCTGGGTTG TCTACAATGA AGTTATACAA 
ACCTCCAAGT ATTATATGAG AGATGTGACT GCTATTGAAT CTGCTTGGCT GTTGGAGCTG 
GCTCCACACT TTTATCAACA AGGAACGGTA GGAATGAACA GAGCCTCTCC CTCAAACCGT 
CTGTTATCTG CTTTGTATTT TGTAAATATA CATTTATTCT CTAATTTGGT TACCAGATCC 
CCAGAACATT CTGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023580454.1
CDS	142..2319
Translation	MAAPVGPVKFWRPGTEGPGVSISEERQSLAENSVTTVVYNAYAALSIEQQRQKLPVFKLRNHILYLIENCQTVVIVGETGCGKSTQIPQYLAEAGWTAEGRVVGVTQPRRVAAVTVAGRVAEERGAVLGHEVGYCIRFDDCTDPLATRIKFLTDGMLVREMMVDPLLTKYSAIMLDEAHERTLYTDIAIGLLKKIQKKRGDLRLIVASATLDAEKFRDFFNQNETSDPTRDTCVILTVEGRTFPVDIFYLQSPVPDYIKSTVETVMKIHQTEGDGDILAFLTGQEEVETVVSMLIEHARELARTGMKRHLRVLPMYSGLPSFEQMKVFERVPRSVRKVIVATNVAETSITISGIVYVIDCGFMKLRAYNPRTAIECLVVVPVSQASANQRAGRGGRNRSGKCYRLYTEEAFDKLPQSTVPEMQRSNLAPVILQLKALGIDNVLRFHFMSPPPAQSMVQALELLYALGGLDKDCRLTEPLGMRIAEFPLNPMFAKMLLESGNFGCSQEILSIAAMMQIQNIFVIPPNQKSQAIRVHRRFAVEEGDHLTMLNVYEAFIKHNKNSQWCQEHFLNYKGLVRAATVREQLKKLLVKFQVPKKSSEGDPDPVLRCIVSGFFANAARFHSSGVYRTTRDDHELHIHPASVLYAEKPPRWVVYNEVIQTSKYYMRDVTAIESAWLLELAPHFYQQGTVGMNRASPSNRLLSALYFVNIHLFSNLVTRSPEHSG

Target ORF information:

RefSeq Version	XM_023724686.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris DEAH-box helicase 35 (LOC101360377), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023724686.1

ORF Insert Sequence:

ATGGCTGCGC CCGTGGGACC AGTGAAGTTC TGGCGACCCG GTACGGAGGG GCCAGGTGTC 
AGCATCTCTG AAGAGAGACA AAGTCTAGCG GAAAACTCTG TGACAACTGT TGTTTACAAT 
GCTTACGCTG CCCTTTCTAT AGAGCAACAG AGGCAGAAGC TGCCGGTTTT CAAGCTTAGG 
AATCATATAT TATACTTGAT AGAAAACTGT CAGACGGTGG TGATTGTTGG AGAAACGGGA 
TGTGGAAAAA GCACACAGAT CCCACAATAC CTTGCAGAAG CTGGCTGGAC AGCCGAAGGA 
AGAGTTGTGG GCGTGACCCA GCCTCGAAGA GTGGCTGCCG TGACAGTTGC AGGGAGAGTA 
GCTGAGGAAA GAGGTGCAGT GCTGGGCCAC GAGGTGGGCT ACTGCATCCG CTTTGACGAC 
TGCACTGACC CGCTGGCTAC AAGAATTAAG TTTCTGACCG ATGGAATGCT GGTCAGGGAA 
ATGATGGTTG ACCCTTTGTT AACAAAATAT AGTGCCATCA TGCTGGATGA AGCTCATGAG 
AGGACCTTGT ACACCGACAT TGCCATTGGC TTGTTAAAAA AGATTCAGAA AAAGCGAGGG 
GATCTCCGCT TGATTGTGGC CTCAGCCACT CTGGATGCAG AGAAATTTCG AGATTTCTTT 
AATCAAAATG AAACCAGTGA CCCGACCAGA GATACGTGTG TGATCCTTAC AGTGGAAGGG 
CGAACGTTTC CAGTGGATAT CTTTTATCTG CAAAGTCCTG TTCCAGATTA TATCAAATCA 
ACTGTGGAAA CTGTGATGAA AATTCACCAG ACAGAGGGCG ATGGAGACAT ATTAGCATTT 
CTTACTGGCC AGGAAGAGGT AGAAACTGTT GTGTCTATGC TAATCGAGCA CGCTCGAGAG 
CTGGCTCGCA CTGGGATGAA GAGACACCTC CGAGTTCTCC CCATGTATTC AGGACTGCCT 
TCCTTTGAGC AAATGAAAGT GTTTGAACGA GTGCCACGCA GTGTCAGGAA GGTGATAGTG 
GCCACCAATG TGGCAGAAAC CTCCATCACC ATCAGCGGGA TTGTATATGT GATTGACTGC 
GGCTTCATGA AACTCCGTGC CTACAATCCC AGAACAGCTA TTGAATGCTT GGTGGTGGTC 
CCGGTGTCCC AGGCCTCAGC CAACCAGCGA GCAGGGCGTG GTGGTCGTAA CCGCTCAGGG 
AAATGTTATC GACTTTATAC AGAGGAAGCC TTTGATAAGT TGCCTCAATC CACCGTTCCT 
GAGATGCAGC GCAGCAATTT GGCGCCTGTC ATCCTGCAGC TGAAGGCGCT GGGAATTGAC 
AATGTCCTCA GGTTCCACTT CATGTCGCCT CCTCCAGCAC AGTCAATGGT TCAAGCTTTG 
GAGTTACTCT ATGCTCTGGG AGGTCTGGAC AAAGACTGTC GGCTAACTGA ACCGCTTGGC 
ATGAGGATAG CGGAGTTTCC TTTGAATCCC ATGTTTGCAA AAATGCTGCT TGAATCAGGA 
AATTTTGGCT GTTCTCAGGA AATCCTAAGC ATTGCGGCCA TGATGCAGAT CCAGAATATC 
TTTGTGATCC CCCCAAACCA GAAGTCCCAG GCAATTCGAG TGCACCGAAG ATTTGCTGTG 
GAGGAGGGTG ATCATCTCAC TATGCTCAAC GTGTATGAAG CATTCATCAA ACACAATAAG 
AACTCGCAGT GGTGTCAGGA ACATTTCCTG AATTACAAGG GCCTTGTCAG AGCTGCAACG 
GTGCGAGAAC AATTGAAAAA GCTTCTTGTC AAGTTCCAAG TGCCCAAGAA ATCTAGTGAA 
GGTGATCCGG ATCCGGTTCT GAGGTGCATT GTCTCGGGAT TCTTTGCAAA TGCTGCGAGG 
TTTCATTCTT CTGGAGTTTA TAGGACTACC CGTGATGACC ACGAATTGCA CATCCACCCT 
GCATCAGTCC TCTATGCAGA GAAGCCGCCT CGCTGGGTTG TCTACAATGA AGTTATACAA 
ACCTCCAAGT ATTATATGAG AGATGTGACT GCTATTGAAT CTGCTTGGCT GTTGGAGCTG 
GCTCCACACT TTTATCAACA AGGAACGGTA GGAATGAACA GAGCCTCTCC CTCAAACCGT 
CTGTTATCTG CTTTGTATTT TGTAAATATA CATTTATTCT CTAATTTGGT TACCAGATCC 
CCAGAACATT CTGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

LOC101360377 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム