LOC101344341 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101344341
Entrez Gene ID	101344341
Full Name	cysteine rich transmembrane BMP regulator 1
Synonyms	CRIM1
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004369565.2	XP_004369622.1	cysteine-rich motor neuron 1 protein
XM_004369565.1	XP_004369622.1	cysteine-rich motor neuron 1 protein

Pathways from BioSystems
Gene Ontology

The following LOC101344341 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101344341 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTr165654	XM_004369565.2 Latest version!	Trichechus manatus latirostris cysteine rich transmembrane BMP regulator 1 (LOC101344341), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OTr165654	XM_004369565.1	Trichechus manatus latirostris cysteine rich transmembrane BMP regulator 1 (chordin-like) (LOC101344341), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101344341 ( XM_004369565.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004369622.1 Trichechus manatus latirostris cysteine rich transmembrane BMP regulator 1 (LOC101344341), mRNA.

Price & Availability↑

CloneID	OTr165654
Clone ID Related Accession (Same CDS sequence)	XM_004369565.2 , XM_004369565.1
Accession Version	XM_004369565.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443941.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 25, 2018 this sequence version replaced XM_004369565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTCGGGCTGC TGCTGCTTCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGT 
GACGAGTCCA AGTGCGAAGA ACCCAGGAAC TGCCCCGGGA GCATCGTGCA GGGCGTCTGT 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTG 
CACGGAGCCT GCGACCGGGG GCTACGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAAAACT GGGATGACGA CCAACTGCTT 
GGTTTTGAAC CATGCAATGA AAACCTCATC TCTGGCTGCA ATATAATCAA TGGGAAATGT 
GAATGTGACA CCATTCGAAC CTGCAGCAGT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTC TAAAGAGAAT TGAAGAAGAG AAGCCAGATT GCACCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCGCGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGTTA CGCTCCTCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGC GTGTGCGACC CTGCAGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCCAAAG CGTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTT TTCAGCGTGG ACTGCAGCAC TGTGGAATGC 
CCCTCTGTTC AGCAGGCTGT GTGCCCCCTG GATAGCTACG AAACTCAAGT TAGACTCACA 
GCTGATGGGT GCTGTACTCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCATGTGTG AGGTGGGATC CACTCCCCGC ATAGTCTCTC GTGGAGATGG GACACCCGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGG CAGCCTGTGT GTTCAACAAC 
GTGGAATATT ATGATGGAGA TATGTTCCGA ATGGACAACT GTCGATTCTG TCGATGCCAA 
GGAGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGCGAGC TGAACTGTGA GAGGTACTAC 
GTGCCCGAAG GGGAGTGCTG CCCAGTTTGT GAAGATCCAG TATATCCTCT TAACAAGCCT 
GCTGGCTGCT ATGCCAGTGG CCAGATCCGC GCCCACGGAG ACCGGTGGAG GGAAGACGAC 
TGCACGTTCT GCCAGTGCAT CAATGGAGAG CCCCACTGCG TCGCAACAGC CTGCGGGCAG 
AGCTGCATGA ACCCCGTAAA AGTGCCTGGG GAGTGTTGCC CTGTGTGCGA AGAACCAACC 
TATATCACAA TTGATCCACC TGCATGTGGG GAGCTATCAA ATTGCACCCT GACGGAGAAG 
GACTGCATTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACTTGTCA GTGCAAAAAC 
AGGGAGGATC TGTGCACCGG CCTCAAACGA GGGTGCACCT TGGATTGTCC CTTCGGTTTC 
CAAACTGATG CCCACAACTG TGAGATCTGC CAGTGCCGCC CACGGCCAAA GAAGTGCAAG 
CCTGTAATCT GTGACAAGTA CTGTCCATTT GGATACCTGA AGAATAAGCA CGGCTGTGAC 
ATCTGTCGCT GTAAGAAATG TCCAGAACTC CCATGCAGTA AAATCTGCCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTACCTGC AAGTGCAGAG AGGTCCCTGC TTCAGCTGGG 
CCACCTGTCC TGTCAGGCAC ATGTCTGTCC ATGGATGGCC ATCATCATAA AAATGAGGAA 
AGCTGGCACG ATGGGTGCCG GGAGTGCTAC TGTCACAATG GACGGGAAAT GTGTGCCCTG 
ATCACCTGCC CGGTGCCTGC CTGTGGCAAC CCCACCATTC ACCCGGGACA GTGCTGCCCA 
TCATGTTCAG ATGACTTTGT GGTGCAGAAG CCGGAGCTTA GCACCCCCTC CATTTGCCAC 
GCACCCGGAG GAGAGTACTT TGTGGAAGGA GAAACGTGGA ACATCGACTC CTGCACTCAG 
TGCACCTGCC GCAGCGGGCG GGTGCTATGT GAGACGGAGG TGTGCCCACC GCTGCTCTGC 
CAGAATCCCT CACGCACACA GGACTCCTGC TGCCCACAGT GTACAGATGA ACCTCTTCAG 
CCTTCTTCAT CCCATAATGA CAGCGTGCCT AGTTACTGCA AAAATGATGA AGGGGATATA 
TTCCTGGCAG CTGAGTCCTG GAAGCCTGAC GTTTGTACCA GCTGTGTATG CATGGATAGC 
GTAATTAGCT GTTTCTCTGA GTCTTGCCCT TCAGTATCCT GTGAAAGACC CGTTTTGAGA 
AAGGGCCAGT GTTGTCCCTA CTGCATAGAA GACACAATCC CGAAGAAGGT GGTGTGCCAC 
TTCAGCGGGA AGGCCTATGC TGACGAAGAG CGGTGGGACA TTGACAGCTG CACACACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCG ACAGTCAGCT GCCCCCCTCT GCCCTGTGTC 
GAGCCCATCA ACGTGGAGGG AAGTTGCTGC CCAATGTGTC CAGAAATGTA TGTCCCGGAA 
CCAACCAACA TACCCATTGA GAAGACAAAC CATCATGGAG AGATTGACCT GGGGGTTCCC 
ATGTGGCCCA CACCTAGTGA AAATGACATC ATCCATCTCC CTATAGACAT GGGTCATCTC 
CAGGTAGATT ACAGAGATAA CAACAGGCTG CACCCAAGCG AAGAGTCTTC GCTGGATTCC 
ACCACTTCGG TCGTGGTTCC CATCATCATC TGCCTCTCTA TCATCATAGC ATTCCTGTTC 
ATCAATCAGA AGAAACAGTG GATCCCACTG CTCTGCTGGT ATCGGACACC AACCAAGCCT 
TCTTCCTTAA ATAATCAGCT GGTATCTGTG GACTGCAAGA AAGGAACTCG AGTCCCAGTG 
GACAGTTCCC AGAGAATGCT AAGAATTGCT GAACCAGATG CAAGATTCAG TGGCTTCTAC 
AGCATGCAAA AACAAAACCA TCTACAGGCA GACAATTTCT ACCAAACAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004369622.1
CDS	61..3174
Translation	MYLVAGGRGLAGCGHLLVSLLGLLLLLARSGTRALVCLPCDESKCEEPRNCPGSIVQGVCGCCYMCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLISGCNIINGKCECDTIRTCSSPFEFPRKDMCLSALKRIEEEKPDCTKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSVQQAVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPMCEVGSTPRIVSRGDGTPGKCCDVFECVNETKAACVFNNVEYYDGDMFRMDNCRFCRCQGGVSICFTAQCGELNCERYYVPEGECCPVCEDPVYPLNKPAGCYASGQIRAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCIYGFKLDHNGCRTCQCKNREDLCTGLKRGCTLDCPFGFQTDAHNCEICQCRPRPKKCKPVICDKYCPFGYLKNKHGCDICRCKKCPELPCSKICPLGFQQDSHGCLTCKCREVPASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHNGREMCALITCPVPACGNPTIHPGQCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWNIDSCTQCTCRSGRVLCETEVCPPLLCQNPSRTQDSCCPQCTDEPLQPSSSHNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCFSESCPSVSCERPVLRKGQCCPYCIEDTIPKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHHGEIDLGVPMWPTPSENDIIHLPIDMGHLQVDYRDNNRLHPSEESSLDSTTSVVVPIIICLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGTRVPVDSSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_004369565.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris cysteine rich transmembrane BMP regulator 1 (LOC101344341), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004369565.2

ORF Insert Sequence:

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTCGGGCTGC TGCTGCTTCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGT 
GACGAGTCCA AGTGCGAAGA ACCCAGGAAC TGCCCCGGGA GCATCGTGCA GGGCGTCTGT 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTG 
CACGGAGCCT GCGACCGGGG GCTACGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAAAACT GGGATGACGA CCAACTGCTT 
GGTTTTGAAC CATGCAATGA AAACCTCATC TCTGGCTGCA ATATAATCAA TGGGAAATGT 
GAATGTGACA CCATTCGAAC CTGCAGCAGT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTC TAAAGAGAAT TGAAGAAGAG AAGCCAGATT GCACCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCGCGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGTTA CGCTCCTCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGC GTGTGCGACC CTGCAGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCCAAAG CGTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTT TTCAGCGTGG ACTGCAGCAC TGTGGAATGC 
CCCTCTGTTC AGCAGGCTGT GTGCCCCCTG GATAGCTACG AAACTCAAGT TAGACTCACA 
GCTGATGGGT GCTGTACTCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCATGTGTG AGGTGGGATC CACTCCCCGC ATAGTCTCTC GTGGAGATGG GACACCCGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGG CAGCCTGTGT GTTCAACAAC 
GTGGAATATT ATGATGGAGA TATGTTCCGA ATGGACAACT GTCGATTCTG TCGATGCCAA 
GGAGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGCGAGC TGAACTGTGA GAGGTACTAC 
GTGCCCGAAG GGGAGTGCTG CCCAGTTTGT GAAGATCCAG TATATCCTCT TAACAAGCCT 
GCTGGCTGCT ATGCCAGTGG CCAGATCCGC GCCCACGGAG ACCGGTGGAG GGAAGACGAC 
TGCACGTTCT GCCAGTGCAT CAATGGAGAG CCCCACTGCG TCGCAACAGC CTGCGGGCAG 
AGCTGCATGA ACCCCGTAAA AGTGCCTGGG GAGTGTTGCC CTGTGTGCGA AGAACCAACC 
TATATCACAA TTGATCCACC TGCATGTGGG GAGCTATCAA ATTGCACCCT GACGGAGAAG 
GACTGCATTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACTTGTCA GTGCAAAAAC 
AGGGAGGATC TGTGCACCGG CCTCAAACGA GGGTGCACCT TGGATTGTCC CTTCGGTTTC 
CAAACTGATG CCCACAACTG TGAGATCTGC CAGTGCCGCC CACGGCCAAA GAAGTGCAAG 
CCTGTAATCT GTGACAAGTA CTGTCCATTT GGATACCTGA AGAATAAGCA CGGCTGTGAC 
ATCTGTCGCT GTAAGAAATG TCCAGAACTC CCATGCAGTA AAATCTGCCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTACCTGC AAGTGCAGAG AGGTCCCTGC TTCAGCTGGG 
CCACCTGTCC TGTCAGGCAC ATGTCTGTCC ATGGATGGCC ATCATCATAA AAATGAGGAA 
AGCTGGCACG ATGGGTGCCG GGAGTGCTAC TGTCACAATG GACGGGAAAT GTGTGCCCTG 
ATCACCTGCC CGGTGCCTGC CTGTGGCAAC CCCACCATTC ACCCGGGACA GTGCTGCCCA 
TCATGTTCAG ATGACTTTGT GGTGCAGAAG CCGGAGCTTA GCACCCCCTC CATTTGCCAC 
GCACCCGGAG GAGAGTACTT TGTGGAAGGA GAAACGTGGA ACATCGACTC CTGCACTCAG 
TGCACCTGCC GCAGCGGGCG GGTGCTATGT GAGACGGAGG TGTGCCCACC GCTGCTCTGC 
CAGAATCCCT CACGCACACA GGACTCCTGC TGCCCACAGT GTACAGATGA ACCTCTTCAG 
CCTTCTTCAT CCCATAATGA CAGCGTGCCT AGTTACTGCA AAAATGATGA AGGGGATATA 
TTCCTGGCAG CTGAGTCCTG GAAGCCTGAC GTTTGTACCA GCTGTGTATG CATGGATAGC 
GTAATTAGCT GTTTCTCTGA GTCTTGCCCT TCAGTATCCT GTGAAAGACC CGTTTTGAGA 
AAGGGCCAGT GTTGTCCCTA CTGCATAGAA GACACAATCC CGAAGAAGGT GGTGTGCCAC 
TTCAGCGGGA AGGCCTATGC TGACGAAGAG CGGTGGGACA TTGACAGCTG CACACACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCG ACAGTCAGCT GCCCCCCTCT GCCCTGTGTC 
GAGCCCATCA ACGTGGAGGG AAGTTGCTGC CCAATGTGTC CAGAAATGTA TGTCCCGGAA 
CCAACCAACA TACCCATTGA GAAGACAAAC CATCATGGAG AGATTGACCT GGGGGTTCCC 
ATGTGGCCCA CACCTAGTGA AAATGACATC ATCCATCTCC CTATAGACAT GGGTCATCTC 
CAGGTAGATT ACAGAGATAA CAACAGGCTG CACCCAAGCG AAGAGTCTTC GCTGGATTCC 
ACCACTTCGG TCGTGGTTCC CATCATCATC TGCCTCTCTA TCATCATAGC ATTCCTGTTC 
ATCAATCAGA AGAAACAGTG GATCCCACTG CTCTGCTGGT ATCGGACACC AACCAAGCCT 
TCTTCCTTAA ATAATCAGCT GGTATCTGTG GACTGCAAGA AAGGAACTCG AGTCCCAGTG 
GACAGTTCCC AGAGAATGCT AAGAATTGCT GAACCAGATG CAAGATTCAG TGGCTTCTAC 
AGCATGCAAA AACAAAACCA TCTACAGGCA GACAATTTCT ACCAAACAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101344341 ( XM_004369565.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004369622.1 Trichechus manatus latirostris cysteine rich transmembrane BMP regulator 1 (chordin-like) (LOC101344341), mRNA.

Price & Availability↑

CloneID	OTr165654
Clone ID Related Accession (Same CDS sequence)	XM_004369565.2 , XM_004369565.1
Accession Version	XM_004369565.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443941.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTCGGGCTGC TGCTGCTTCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGT 
GACGAGTCCA AGTGCGAAGA ACCCAGGAAC TGCCCCGGGA GCATCGTGCA GGGCGTCTGT 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTG 
CACGGAGCCT GCGACCGGGG GCTACGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAAAACT GGGATGACGA CCAACTGCTT 
GGTTTTGAAC CATGCAATGA AAACCTCATC TCTGGCTGCA ATATAATCAA TGGGAAATGT 
GAATGTGACA CCATTCGAAC CTGCAGCAGT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTC TAAAGAGAAT TGAAGAAGAG AAGCCAGATT GCACCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCGCGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGTTA CGCTCCTCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGC GTGTGCGACC CTGCAGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCCAAAG CGTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTT TTCAGCGTGG ACTGCAGCAC TGTGGAATGC 
CCCTCTGTTC AGCAGGCTGT GTGCCCCCTG GATAGCTACG AAACTCAAGT TAGACTCACA 
GCTGATGGGT GCTGTACTCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCATGTGTG AGGTGGGATC CACTCCCCGC ATAGTCTCTC GTGGAGATGG GACACCCGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGG CAGCCTGTGT GTTCAACAAC 
GTGGAATATT ATGATGGAGA TATGTTCCGA ATGGACAACT GTCGATTCTG TCGATGCCAA 
GGAGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGCGAGC TGAACTGTGA GAGGTACTAC 
GTGCCCGAAG GGGAGTGCTG CCCAGTTTGT GAAGATCCAG TATATCCTCT TAACAAGCCT 
GCTGGCTGCT ATGCCAGTGG CCAGATCCGC GCCCACGGAG ACCGGTGGAG GGAAGACGAC 
TGCACGTTCT GCCAGTGCAT CAATGGAGAG CCCCACTGCG TCGCAACAGC CTGCGGGCAG 
AGCTGCATGA ACCCCGTAAA AGTGCCTGGG GAGTGTTGCC CTGTGTGCGA AGAACCAACC 
TATATCACAA TTGATCCACC TGCATGTGGG GAGCTATCAA ATTGCACCCT GACGGAGAAG 
GACTGCATTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACTTGTCA GTGCAAAAAC 
AGGGAGGATC TGTGCACCGG CCTCAAACGA GGGTGCACCT TGGATTGTCC CTTCGGTTTC 
CAAACTGATG CCCACAACTG TGAGATCTGC CAGTGCCGCC CACGGCCAAA GAAGTGCAAG 
CCTGTAATCT GTGACAAGTA CTGTCCATTT GGATACCTGA AGAATAAGCA CGGCTGTGAC 
ATCTGTCGCT GTAAGAAATG TCCAGAACTC CCATGCAGTA AAATCTGCCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTACCTGC AAGTGCAGAG AGGTCCCTGC TTCAGCTGGG 
CCACCTGTCC TGTCAGGCAC ATGTCTGTCC ATGGATGGCC ATCATCATAA AAATGAGGAA 
AGCTGGCACG ATGGGTGCCG GGAGTGCTAC TGTCACAATG GACGGGAAAT GTGTGCCCTG 
ATCACCTGCC CGGTGCCTGC CTGTGGCAAC CCCACCATTC ACCCGGGACA GTGCTGCCCA 
TCATGTTCAG ATGACTTTGT GGTGCAGAAG CCGGAGCTTA GCACCCCCTC CATTTGCCAC 
GCACCCGGAG GAGAGTACTT TGTGGAAGGA GAAACGTGGA ACATCGACTC CTGCACTCAG 
TGCACCTGCC GCAGCGGGCG GGTGCTATGT GAGACGGAGG TGTGCCCACC GCTGCTCTGC 
CAGAATCCCT CACGCACACA GGACTCCTGC TGCCCACAGT GTACAGATGA ACCTCTTCAG 
CCTTCTTCAT CCCATAATGA CAGCGTGCCT AGTTACTGCA AAAATGATGA AGGGGATATA 
TTCCTGGCAG CTGAGTCCTG GAAGCCTGAC GTTTGTACCA GCTGTGTATG CATGGATAGC 
GTAATTAGCT GTTTCTCTGA GTCTTGCCCT TCAGTATCCT GTGAAAGACC CGTTTTGAGA 
AAGGGCCAGT GTTGTCCCTA CTGCATAGAA GACACAATCC CGAAGAAGGT GGTGTGCCAC 
TTCAGCGGGA AGGCCTATGC TGACGAAGAG CGGTGGGACA TTGACAGCTG CACACACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCG ACAGTCAGCT GCCCCCCTCT GCCCTGTGTC 
GAGCCCATCA ACGTGGAGGG AAGTTGCTGC CCAATGTGTC CAGAAATGTA TGTCCCGGAA 
CCAACCAACA TACCCATTGA GAAGACAAAC CATCATGGAG AGATTGACCT GGGGGTTCCC 
ATGTGGCCCA CACCTAGTGA AAATGACATC ATCCATCTCC CTATAGACAT GGGTCATCTC 
CAGGTAGATT ACAGAGATAA CAACAGGCTG CACCCAAGCG AAGAGTCTTC GCTGGATTCC 
ACCACTTCGG TCGTGGTTCC CATCATCATC TGCCTCTCTA TCATCATAGC ATTCCTGTTC 
ATCAATCAGA AGAAACAGTG GATCCCACTG CTCTGCTGGT ATCGGACACC AACCAAGCCT 
TCTTCCTTAA ATAATCAGCT GGTATCTGTG GACTGCAAGA AAGGAACTCG AGTCCCAGTG 
GACAGTTCCC AGAGAATGCT AAGAATTGCT GAACCAGATG CAAGATTCAG TGGCTTCTAC 
AGCATGCAAA AACAAAACCA TCTACAGGCA GACAATTTCT ACCAAACAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004369622.1
CDS	325..3438
Translation	MYLVAGGRGLAGCGHLLVSLLGLLLLLARSGTRALVCLPCDESKCEEPRNCPGSIVQGVCGCCYMCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLISGCNIINGKCECDTIRTCSSPFEFPRKDMCLSALKRIEEEKPDCTKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSVQQAVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPMCEVGSTPRIVSRGDGTPGKCCDVFECVNETKAACVFNNVEYYDGDMFRMDNCRFCRCQGGVSICFTAQCGELNCERYYVPEGECCPVCEDPVYPLNKPAGCYASGQIRAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCIYGFKLDHNGCRTCQCKNREDLCTGLKRGCTLDCPFGFQTDAHNCEICQCRPRPKKCKPVICDKYCPFGYLKNKHGCDICRCKKCPELPCSKICPLGFQQDSHGCLTCKCREVPASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHNGREMCALITCPVPACGNPTIHPGQCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWNIDSCTQCTCRSGRVLCETEVCPPLLCQNPSRTQDSCCPQCTDEPLQPSSSHNDSVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCFSESCPSVSCERPVLRKGQCCPYCIEDTIPKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHHGEIDLGVPMWPTPSENDIIHLPIDMGHLQVDYRDNNRLHPSEESSLDSTTSVVVPIIICLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGTRVPVDSSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_004369565.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris cysteine rich transmembrane BMP regulator 1 (chordin-like) (LOC101344341), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004369565.1

ORF Insert Sequence:

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTCGGGCTGC TGCTGCTTCT GGCGCGCTCC GGCACCCGGG CGCTGGTCTG CCTGCCCTGT 
GACGAGTCCA AGTGCGAAGA ACCCAGGAAC TGCCCCGGGA GCATCGTGCA GGGCGTCTGT 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTG 
CACGGAGCCT GCGACCGGGG GCTACGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAAAACT GGGATGACGA CCAACTGCTT 
GGTTTTGAAC CATGCAATGA AAACCTCATC TCTGGCTGCA ATATAATCAA TGGGAAATGT 
GAATGTGACA CCATTCGAAC CTGCAGCAGT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTC TAAAGAGAAT TGAAGAAGAG AAGCCAGATT GCACCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCGCGTTG TCCTGAAGAT TCCGTTCTGA TCGAGGGTTA CGCTCCTCCC 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGC GTGTGCGACC CTGCAGGCTG TCTGCGCAAA 
GTCTGCCAGC CGGGATACCT GAACATACTA GTGTCCAAAG CGTCAGGGAA GCCGGGAGAG 
TGTTGTGACC TCTATGAGTG CAAACCAGTT TTCAGCGTGG ACTGCAGCAC TGTGGAATGC 
CCCTCTGTTC AGCAGGCTGT GTGCCCCCTG GATAGCTACG AAACTCAAGT TAGACTCACA 
GCTGATGGGT GCTGTACTCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCATGTGTG AGGTGGGATC CACTCCCCGC ATAGTCTCTC GTGGAGATGG GACACCCGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGG CAGCCTGTGT GTTCAACAAC 
GTGGAATATT ATGATGGAGA TATGTTCCGA ATGGACAACT GTCGATTCTG TCGATGCCAA 
GGAGGTGTTT CCATCTGCTT CACTGCCCAG TGTGGCGAGC TGAACTGTGA GAGGTACTAC 
GTGCCCGAAG GGGAGTGCTG CCCAGTTTGT GAAGATCCAG TATATCCTCT TAACAAGCCT 
GCTGGCTGCT ATGCCAGTGG CCAGATCCGC GCCCACGGAG ACCGGTGGAG GGAAGACGAC 
TGCACGTTCT GCCAGTGCAT CAATGGAGAG CCCCACTGCG TCGCAACAGC CTGCGGGCAG 
AGCTGCATGA ACCCCGTAAA AGTGCCTGGG GAGTGTTGCC CTGTGTGCGA AGAACCAACC 
TATATCACAA TTGATCCACC TGCATGTGGG GAGCTATCAA ATTGCACCCT GACGGAGAAG 
GACTGCATTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACTTGTCA GTGCAAAAAC 
AGGGAGGATC TGTGCACCGG CCTCAAACGA GGGTGCACCT TGGATTGTCC CTTCGGTTTC 
CAAACTGATG CCCACAACTG TGAGATCTGC CAGTGCCGCC CACGGCCAAA GAAGTGCAAG 
CCTGTAATCT GTGACAAGTA CTGTCCATTT GGATACCTGA AGAATAAGCA CGGCTGTGAC 
ATCTGTCGCT GTAAGAAATG TCCAGAACTC CCATGCAGTA AAATCTGCCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTACCTGC AAGTGCAGAG AGGTCCCTGC TTCAGCTGGG 
CCACCTGTCC TGTCAGGCAC ATGTCTGTCC ATGGATGGCC ATCATCATAA AAATGAGGAA 
AGCTGGCACG ATGGGTGCCG GGAGTGCTAC TGTCACAATG GACGGGAAAT GTGTGCCCTG 
ATCACCTGCC CGGTGCCTGC CTGTGGCAAC CCCACCATTC ACCCGGGACA GTGCTGCCCA 
TCATGTTCAG ATGACTTTGT GGTGCAGAAG CCGGAGCTTA GCACCCCCTC CATTTGCCAC 
GCACCCGGAG GAGAGTACTT TGTGGAAGGA GAAACGTGGA ACATCGACTC CTGCACTCAG 
TGCACCTGCC GCAGCGGGCG GGTGCTATGT GAGACGGAGG TGTGCCCACC GCTGCTCTGC 
CAGAATCCCT CACGCACACA GGACTCCTGC TGCCCACAGT GTACAGATGA ACCTCTTCAG 
CCTTCTTCAT CCCATAATGA CAGCGTGCCT AGTTACTGCA AAAATGATGA AGGGGATATA 
TTCCTGGCAG CTGAGTCCTG GAAGCCTGAC GTTTGTACCA GCTGTGTATG CATGGATAGC 
GTAATTAGCT GTTTCTCTGA GTCTTGCCCT TCAGTATCCT GTGAAAGACC CGTTTTGAGA 
AAGGGCCAGT GTTGTCCCTA CTGCATAGAA GACACAATCC CGAAGAAGGT GGTGTGCCAC 
TTCAGCGGGA AGGCCTATGC TGACGAAGAG CGGTGGGACA TTGACAGCTG CACACACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCG ACAGTCAGCT GCCCCCCTCT GCCCTGTGTC 
GAGCCCATCA ACGTGGAGGG AAGTTGCTGC CCAATGTGTC CAGAAATGTA TGTCCCGGAA 
CCAACCAACA TACCCATTGA GAAGACAAAC CATCATGGAG AGATTGACCT GGGGGTTCCC 
ATGTGGCCCA CACCTAGTGA AAATGACATC ATCCATCTCC CTATAGACAT GGGTCATCTC 
CAGGTAGATT ACAGAGATAA CAACAGGCTG CACCCAAGCG AAGAGTCTTC GCTGGATTCC 
ACCACTTCGG TCGTGGTTCC CATCATCATC TGCCTCTCTA TCATCATAGC ATTCCTGTTC 
ATCAATCAGA AGAAACAGTG GATCCCACTG CTCTGCTGGT ATCGGACACC AACCAAGCCT 
TCTTCCTTAA ATAATCAGCT GGTATCTGTG GACTGCAAGA AAGGAACTCG AGTCCCAGTG 
GACAGTTCCC AGAGAATGCT AAGAATTGCT GAACCAGATG CAAGATTCAG TGGCTTCTAC 
AGCATGCAAA AACAAAACCA TCTACAGGCA GACAATTTCT ACCAAACAGT GTGA