E2F1 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	E2F1
Entrez Gene ID	106550369
Full Name	E2F transcription factor 1
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014068270.1	XP_013923745.1	transcription factor E2F1

Pathways from BioSystems
Gene Ontology

The following E2F1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E2F1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh26015	XM_014068270.1 Latest version!	Thamnophis sirtalis E2F transcription factor 1 (E2F1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

E2F1 ( XM_014068270.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013923745.1 Thamnophis sirtalis E2F transcription factor 1 (E2F1), mRNA.

Price & Availability↑

CloneID	OTh26015
Clone ID Related Accession (Same CDS sequence)	XM_014068270.1
Accession Version	XM_014068270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	transcription factor E2F1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013659176.1) and transcript sequences (GDKU01026830.1 and GDKU01026831.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 433 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCTCTGG AGGGGCTGGA AGTAACTCAG AATGTGGCAG ATCTGGAAGC CTTGCTGAGG 
GGAGCGGAGG CCATCCATGT GATGGAACAG CAGATCGTGA TCATTTCCAC TCCAGACCAG 
CTCCCCGCGT CCGAAGGAGA CCACGACACC GAGCTGCTGC TCTTTGCCAC TCCACAGCCC 
GCCAGATTGG AGACTGTAAC GCAGAGGCCT GCTCTGGAAC GGCCACCGGT AAAACGGAAA 
TTGAATTTGG AGACTGACCA TCAATACATT GCAGACCATG CTCAGGTGGC TCGAGGAAAA 
GCAAAAAATC CTATAAAAGG TTTAAAGTCT CCTGGAGAGA AATCCCGCTA TGAAACGTCT 
CTCAACTTGA CCACCAAGCG CTTCTTAGAG CTATTGAGTC AGTCTCCTGA TGGTGTTGTG 
GATCTCAACT GGGCTGCTGA AGTACTAAAG GTGCAGAAGA GGCGTATTTA TGATATAACA 
AATGTCCTGG AGGGCATCCA GCTCATCACC AAGAAATCCA AGAACCATAT CCAATGGCTG 
GGGAACCGGA CTTCTGTGGC GGGCACCAAT AAGAATCAAG TGTTAATGAA GGAACTACAG 
GATCTTCAAG CAGCAGAACA GCATCTGGAC AGCCTCATCC AGATGTGTAC TACTCAATTC 
AAGATCCTTA CTGAGGAACC TGAAAACAAG CATTCAGCTT ATGTGACTTG CCAAGATCTA 
CGCAACATTG CAGACCCCTC AGAGCAACTG GTGATGGTCA TCAAAGCCCC CCCTGAGACA 
CAGATGCAAG TCTCTGATCC TGCAGAGGCT TTCCAGATTG CTGTGAAAAG TACTCAGGGC 
CCTGTTGATG TGTTCCTCTG CCCAGAAGAA AGCTCTGGTG TCTGTAGTCC TACTAAGAGT 
CCATTGAAAG TGGCTCCTGA GGAGCCTTCT TCACAACCTT CACAAACAAC ACCCTCTGTG 
CTTCTACACC CTTCTCAGGA TACAAGTTTG CCATTGCTGA GTGGAGACCA AGAACCTTTG 
TTATCTGGAG AGACTACACT GCCTTTGAAG TGTCCCGGGG ATGATGTAAA CCTCTCACCA 
CTGGCTTCCG TGGATGCCTT GCTTCTGCAG GGCAAGGATG ACTTCTCAAG TTTTCTGCCC 
TCTGAATTCA TTGCACTTTC ACCACCCCAG ACTCAGGACT ATCATTTTGG CCTCGAGGAG 
GGTGAAGGCA TCAGTGAGCT CTTTGACTGT GACTTTGGAG ATTTCACCTC CTTGGATTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013923745.1
CDS	1..1263
Translation	MSLEGLEVTQNVADLEALLRGAEAIHVMEQQIVIISTPDQLPASEGDHDTELLLFATPQPARLETVTQRPALERPPVKRKLNLETDHQYIADHAQVARGKAKNPIKGLKSPGEKSRYETSLNLTTKRFLELLSQSPDGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLITKKSKNHIQWLGNRTSVAGTNKNQVLMKELQDLQAAEQHLDSLIQMCTTQFKILTEEPENKHSAYVTCQDLRNIADPSEQLVMVIKAPPETQMQVSDPAEAFQIAVKSTQGPVDVFLCPEESSGVCSPTKSPLKVAPEEPSSQPSQTTPSVLLHPSQDTSLPLLSGDQEPLLSGETTLPLKCPGDDVNLSPLASVDALLLQGKDDFSSFLPSEFIALSPPQTQDYHFGLEEGEGISELFDCDFGDFTSLDF

Target ORF information:

RefSeq Version	XM_014068270.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis E2F transcription factor 1 (E2F1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014068270.1

ORF Insert Sequence:

ATGTCTCTGG AGGGGCTGGA AGTAACTCAG AATGTGGCAG ATCTGGAAGC CTTGCTGAGG 
GGAGCGGAGG CCATCCATGT GATGGAACAG CAGATCGTGA TCATTTCCAC TCCAGACCAG 
CTCCCCGCGT CCGAAGGAGA CCACGACACC GAGCTGCTGC TCTTTGCCAC TCCACAGCCC 
GCCAGATTGG AGACTGTAAC GCAGAGGCCT GCTCTGGAAC GGCCACCGGT AAAACGGAAA 
TTGAATTTGG AGACTGACCA TCAATACATT GCAGACCATG CTCAGGTGGC TCGAGGAAAA 
GCAAAAAATC CTATAAAAGG TTTAAAGTCT CCTGGAGAGA AATCCCGCTA TGAAACGTCT 
CTCAACTTGA CCACCAAGCG CTTCTTAGAG CTATTGAGTC AGTCTCCTGA TGGTGTTGTG 
GATCTCAACT GGGCTGCTGA AGTACTAAAG GTGCAGAAGA GGCGTATTTA TGATATAACA 
AATGTCCTGG AGGGCATCCA GCTCATCACC AAGAAATCCA AGAACCATAT CCAATGGCTG 
GGGAACCGGA CTTCTGTGGC GGGCACCAAT AAGAATCAAG TGTTAATGAA GGAACTACAG 
GATCTTCAAG CAGCAGAACA GCATCTGGAC AGCCTCATCC AGATGTGTAC TACTCAATTC 
AAGATCCTTA CTGAGGAACC TGAAAACAAG CATTCAGCTT ATGTGACTTG CCAAGATCTA 
CGCAACATTG CAGACCCCTC AGAGCAACTG GTGATGGTCA TCAAAGCCCC CCCTGAGACA 
CAGATGCAAG TCTCTGATCC TGCAGAGGCT TTCCAGATTG CTGTGAAAAG TACTCAGGGC 
CCTGTTGATG TGTTCCTCTG CCCAGAAGAA AGCTCTGGTG TCTGTAGTCC TACTAAGAGT 
CCATTGAAAG TGGCTCCTGA GGAGCCTTCT TCACAACCTT CACAAACAAC ACCCTCTGTG 
CTTCTACACC CTTCTCAGGA TACAAGTTTG CCATTGCTGA GTGGAGACCA AGAACCTTTG 
TTATCTGGAG AGACTACACT GCCTTTGAAG TGTCCCGGGG ATGATGTAAA CCTCTCACCA 
CTGGCTTCCG TGGATGCCTT GCTTCTGCAG GGCAAGGATG ACTTCTCAAG TTTTCTGCCC 
TCTGAATTCA TTGCACTTTC ACCACCCCAG ACTCAGGACT ATCATTTTGG CCTCGAGGAG 
GGTGAAGGCA TCAGTGAGCT CTTTGACTGT GACTTTGGAG ATTTCACCTC CTTGGATTTC 
TGA