IARS cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	IARS
Entrez Gene ID	106544440
Full Name	isoleucyl-tRNA synthetase
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014060707.1	XP_013916182.1	isoleucine--tRNA ligase, cytoplasmic

Pathways from BioSystems
Gene Ontology

The following IARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh20285	XM_014060707.1 Latest version!	Thamnophis sirtalis isoleucyl-tRNA synthetase (IARS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IARS ( XM_014060707.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013916182.1 Thamnophis sirtalis isoleucyl-tRNA synthetase (IARS), mRNA.

Price & Availability↑

CloneID	OTh20285
Clone ID Related Accession (Same CDS sequence)	XM_014060707.1
Accession Version	XM_014060707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	isoleucine--tRNA ligase, cytoplasmic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013658316.1) and transcript sequence (GDKU01080135.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1110 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGGGATTG CAGAATATAA TCGCAACTGT CGAGGAATTG TGATGAGATA TTCTCAAGAA 
TGGAGGACCA GTGTTACAAG ACTTGGGCGG TGGATTGATT TTGACAATGA TTATAAAACT 
CTTTATCCAG AATTCATGGA GTCTGTCTGG TGGGTTTTCA AACAGCTATA TGACAAAGGA 
TTGGTATACC GAGGAGTTAA GGTTATGCCG TTCTCAACTG CATGTAATAC ACCCCTTTCC 
AACTTTGAGT CTCACCAGAA TTATAAGGAT GTTCAAGATC CCTCAGTTAT TGTCACCTTT 
CCATTGGAAG ATGATGAAAG TATCTCTCTT GTTGCTTGGA CGACAACTCC ATGGACTTTG 
CCTAGCAACT TAGCCCTGTG TGTTAATCCA GATTTGCAGT ATGTGAAAGT GAAAGACCAA 
AATACTGGGA AAAAATTCAT ATTGATGGAA GCTAGATTGG CATCTCTCTA CAAATCTGAA 
AGTGAATATG AAGTACTTGA CAGGTTTCCT GGTGTTACAC TAAAAGGAAA GAAATACAAG 
CCTCTTTTCA ATTACTTTAT AAAGTGCAGA GAAAATGGTG CTTTTTCTGT AGTGACTGAC 
AACTATGTGA AGGAAGAAGA AGGTACAGGA GTTGTACATC AAGCTCCTTA CTTTGGTGCT 
GATGATTATA GGGTCTGCAT GGATTTTAAT ATTATTCAGA AGGATTCAGT GCCTGTTTGT 
CCTGTTGATG CTTCAGGATG TTTTACAGCA GAAGTGACAG ACTTCTCTGG ACAGTACGTC 
AAGGACGCAG ATAAAAATAT AATCAAAGTG CTAAAGGAAC GAGGAAGATT GGTCCATATC 
AGCACTTTTA AGCACAGCTA TCCTTTCTGC TGGAGGTCAG ATACACCTCT GATATATAAA 
GCGGTTCCAA GCTGGTTTGT TCGTGTGGAA CACATGGTGG AAAAGTTGCT GGAAAACAAT 
TCTCAATGTT ATTGGGTTCC TGACTTCGTG AGAGAGAAGC GGTTTGGCAA CTGGTTGAGA 
GATGCACGGG ATTGGGCTAT TTCACGAAAC CGTTATTGGG GAACACCTAT TCCATTGTGG 
GTCAGTGATG ATTTTGAAGA GGTGGTTTGT ATTGGGTCTG TAGCAGAACT GGAAGAACTG 
TCAGGAGTGA AAATCACTGA TCTCCACAGA GAAAGTGTTG ATCACATAAC TATCCCTTCA 
TGCTGTGGCA AAGGTGTCTT ACATCGTGTT TCAGAAGTGT TTGACTGCTG GTTTGAAAGT 
GGAAGTATGC CTTATGCCCA AGTCCATTAT CCATTTGAGA ATAAGAGAGA ATTTGAAGAT 
GCTTTTCCTG CTGATTTCAT AGCTGAAGGC ATTGACCAAA CCAGAGGATG GTTTTACACT 
TTGCTGGTGT TATCGTCAGC CCTCTTCGGA AAGCCTCCTT TCAAAAATGT AATTGTAAAT 
GGCCTAGTTC TTGCCAGTGA TGGGCAAAAG ATGAGCAAAC GAAAGAAGAA TTACCCAGAT 
CCAACAAAAA TTGTCAACTC TTATGGAGCT GATGCACTGA GGTTGTACCT TATCAATTCT 
CCAGTTGTAA GAGCAGAAAA CCTAAGATTC AAGGAGGAAG GTGTAAGAGA TGTGCTGAAA 
GATGTTTTCC TGCCCTGGTA TAATGCCTAT CGCTTTCTGA TTCAGAATGT TGTCATTCTG 
CAACTCAAGA AAGATGGGAA GGAATTCCTT TACAATGAGA ATACAATTAA AGAAAGCAAC 
AACATCATGG ATAAATGGAT TCTTTCCTTC ACACAATCAC TGATTCAGTT CTTCAGGGCT 
GAGATGGCTG CCTACAGACT GTACACAGTA GTTCCTCGAC TGGTTAAATT TGTGGACATT 
CTAACCAACT GGTATGTCAG AATGAATCGA CGGAGGTTAA AGGGTGAGAA TGGAACAGAA 
GACTGCATTA TGGCCTTAGA AACCTTATTC AGCGTCCTCT ACTCCATGTG CAGACTTATG 
GCACCTTATA CTCCGTTTAT TACCGAACTA ATGTACCAGA ATTTGAAGAC TCTTATTCAT 
CCTGCTTGTA TTCAGGAGAA GAATACTGAT AGCATTCACT ACCTCATGCT TCCTCAAGTT 
AGGGAAGACC TGATCGACAA AAACATAGAA AGTGCTGTTT CTTGGATGCA GTCTGTTATT 
GAGCTGGGAC GTGTAATCAG AGATAGGAAA ACCATCCCAG TAAAGTATCC ATTGAAAGAA 
GTGGTCATCA TCCATCAGGA TTCTCAAGCA CTGGAAGACA TCAAGTCCTT GGAAAAGTAT 
ATACTTGAGG AACTTAATGT CCGCAAAGTG ACTTTATCCA CTGATAAAGA TAAGTATGGA 
ATCCGTTTGA GAGCAGAACC TGATCATATG GTGCTTGGGA AGCGACTTAA AGGTGCCTTT 
AAATCTGTAA TGGCTGCTAT CAAAGAATTG CCAAGTGAAC TGTTGGAAGA ATTTCAGAAG 
ACAGGCAAAA TGGTTGTAGA AGGGCATGAA TTGCATGAGG AAGATCTCCG TCTGATGTAC 
ACCTTTGACC AGACTGGAGG AGGATATGGC CAGTTTGAAG CTCATTCTGA TTCTCAGGTT 
TTGGTTCTCC TAGATATTAC TCCTGATCAG TCTATGGTTG ATGAAGGCGT TGCACGGGAA 
GTAATAAATC GCATTCAAAA ACTTCGAAAG AAGCGCAATC TGGTTCCTAC AGATGAGATT 
ACAGTATATT ATCAGGCATC TCCAGAAGGT GATTATCTTG ATACTGTTAT TAAGGAACAT 
ACAGATTTTA TTTTTGCCAC AATAAAAACT GCTTTGAAGC CATATCCAGT GTCTCCCTCA 
GAGGAAGTGC TTATTCAAGA AAAAACTCAG CTGAAGGGAT CAGAACTGGA AATTACAATA 
GCCAAAGCAG CTGTTCATCA CTGTACTGAA CCAGCATGTG CTTATGTCAC TCTGAACATC 
TGCATTAATG GTAAAGAACA AGATGGTATG GTGTTGCTGG AAAATCCGAA GGGAGACAAT 
AAATTAGACT TCACCAAATT TGTTAATACT ATTGCGTGCA TTTTTGGCCT TGAAAAAGCA 
AAAGTAGCAA TTTATAGTGG AAAACAAGAA GTGAAAAAAC AAACAGATTT GCTGAGTCTT 
AATGGAAAGA AGCTTCATGT AACTGCAGGG CCATTGCCAA TTACAAACAG TATTGATGAC 
TTTTTATGTC AGTATATCAA TTTACAACTA GTGAATGCAA AACCACAAGA GTGTCTAAAT 
GGAACGGTGG GAACCCTTCT TACGGAAAAC CCTGTTGGTC AAAATGGATT AACATATCAA 
GGGCTTCTGC ATGAAACAGG CAAAATGTTC GGACTCAGAA GCAGGAGGCT AAAATTGTTC 
TTGGATGAAG CGCTGACAAA AGAGGTTACA AAGGAGACTT CCATGAAGTT CTTAAATACA 
AAGACTCTGT ATGTTCATGC TATACCAACA ACAGCTGAAT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013916182.1
CDS	328..3792
Translation	MGIAEYNRNCRGIVMRYSQEWRTSVTRLGRWIDFDNDYKTLYPEFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVIVTFPLEDDESISLVAWTTTPWTLPSNLALCVNPDLQYVKVKDQNTGKKFILMEARLASLYKSESEYEVLDRFPGVTLKGKKYKPLFNYFIKCRENGAFSVVTDNYVKEEEGTGVVHQAPYFGADDYRVCMDFNIIQKDSVPVCPVDASGCFTAEVTDFSGQYVKDADKNIIKVLKERGRLVHISTFKHSYPFCWRSDTPLIYKAVPSWFVRVEHMVEKLLENNSQCYWVPDFVREKRFGNWLRDARDWAISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGVKITDLHRESVDHITIPSCCGKGVLHRVSEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLSSALFGKPPFKNVIVNGLVLASDGQKMSKRKKNYPDPTKIVNSYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVFLPWYNAYRFLIQNVVILQLKKDGKEFLYNENTIKESNNIMDKWILSFTQSLIQFFRAEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGTEDCIMALETLFSVLYSMCRLMAPYTPFITELMYQNLKTLIHPACIQEKNTDSIHYLMLPQVREDLIDKNIESAVSWMQSVIELGRVIRDRKTIPVKYPLKEVVIIHQDSQALEDIKSLEKYILEELNVRKVTLSTDKDKYGIRLRAEPDHMVLGKRLKGAFKSVMAAIKELPSELLEEFQKTGKMVVEGHELHEEDLRLMYTFDQTGGGYGQFEAHSDSQVLVLLDITPDQSMVDEGVAREVINRIQKLRKKRNLVPTDEITVYYQASPEGDYLDTVIKEHTDFIFATIKTALKPYPVSPSEEVLIQEKTQLKGSELEITIAKAAVHHCTEPACAYVTLNICINGKEQDGMVLLENPKGDNKLDFTKFVNTIACIFGLEKAKVAIYSGKQEVKKQTDLLSLNGKKLHVTAGPLPITNSIDDFLCQYINLQLVNAKPQECLNGTVGTLLTENPVGQNGLTYQGLLHETGKMFGLRSRRLKLFLDEALTKEVTKETSMKFLNTKTLYVHAIPTTAEC

Target ORF information:

RefSeq Version	XM_014060707.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis isoleucyl-tRNA synthetase (IARS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014060707.1

ORF Insert Sequence:

ATGGGGATTG CAGAATATAA TCGCAACTGT CGAGGAATTG TGATGAGATA TTCTCAAGAA 
TGGAGGACCA GTGTTACAAG ACTTGGGCGG TGGATTGATT TTGACAATGA TTATAAAACT 
CTTTATCCAG AATTCATGGA GTCTGTCTGG TGGGTTTTCA AACAGCTATA TGACAAAGGA 
TTGGTATACC GAGGAGTTAA GGTTATGCCG TTCTCAACTG CATGTAATAC ACCCCTTTCC 
AACTTTGAGT CTCACCAGAA TTATAAGGAT GTTCAAGATC CCTCAGTTAT TGTCACCTTT 
CCATTGGAAG ATGATGAAAG TATCTCTCTT GTTGCTTGGA CGACAACTCC ATGGACTTTG 
CCTAGCAACT TAGCCCTGTG TGTTAATCCA GATTTGCAGT ATGTGAAAGT GAAAGACCAA 
AATACTGGGA AAAAATTCAT ATTGATGGAA GCTAGATTGG CATCTCTCTA CAAATCTGAA 
AGTGAATATG AAGTACTTGA CAGGTTTCCT GGTGTTACAC TAAAAGGAAA GAAATACAAG 
CCTCTTTTCA ATTACTTTAT AAAGTGCAGA GAAAATGGTG CTTTTTCTGT AGTGACTGAC 
AACTATGTGA AGGAAGAAGA AGGTACAGGA GTTGTACATC AAGCTCCTTA CTTTGGTGCT 
GATGATTATA GGGTCTGCAT GGATTTTAAT ATTATTCAGA AGGATTCAGT GCCTGTTTGT 
CCTGTTGATG CTTCAGGATG TTTTACAGCA GAAGTGACAG ACTTCTCTGG ACAGTACGTC 
AAGGACGCAG ATAAAAATAT AATCAAAGTG CTAAAGGAAC GAGGAAGATT GGTCCATATC 
AGCACTTTTA AGCACAGCTA TCCTTTCTGC TGGAGGTCAG ATACACCTCT GATATATAAA 
GCGGTTCCAA GCTGGTTTGT TCGTGTGGAA CACATGGTGG AAAAGTTGCT GGAAAACAAT 
TCTCAATGTT ATTGGGTTCC TGACTTCGTG AGAGAGAAGC GGTTTGGCAA CTGGTTGAGA 
GATGCACGGG ATTGGGCTAT TTCACGAAAC CGTTATTGGG GAACACCTAT TCCATTGTGG 
GTCAGTGATG ATTTTGAAGA GGTGGTTTGT ATTGGGTCTG TAGCAGAACT GGAAGAACTG 
TCAGGAGTGA AAATCACTGA TCTCCACAGA GAAAGTGTTG ATCACATAAC TATCCCTTCA 
TGCTGTGGCA AAGGTGTCTT ACATCGTGTT TCAGAAGTGT TTGACTGCTG GTTTGAAAGT 
GGAAGTATGC CTTATGCCCA AGTCCATTAT CCATTTGAGA ATAAGAGAGA ATTTGAAGAT 
GCTTTTCCTG CTGATTTCAT AGCTGAAGGC ATTGACCAAA CCAGAGGATG GTTTTACACT 
TTGCTGGTGT TATCGTCAGC CCTCTTCGGA AAGCCTCCTT TCAAAAATGT AATTGTAAAT 
GGCCTAGTTC TTGCCAGTGA TGGGCAAAAG ATGAGCAAAC GAAAGAAGAA TTACCCAGAT 
CCAACAAAAA TTGTCAACTC TTATGGAGCT GATGCACTGA GGTTGTACCT TATCAATTCT 
CCAGTTGTAA GAGCAGAAAA CCTAAGATTC AAGGAGGAAG GTGTAAGAGA TGTGCTGAAA 
GATGTTTTCC TGCCCTGGTA TAATGCCTAT CGCTTTCTGA TTCAGAATGT TGTCATTCTG 
CAACTCAAGA AAGATGGGAA GGAATTCCTT TACAATGAGA ATACAATTAA AGAAAGCAAC 
AACATCATGG ATAAATGGAT TCTTTCCTTC ACACAATCAC TGATTCAGTT CTTCAGGGCT 
GAGATGGCTG CCTACAGACT GTACACAGTA GTTCCTCGAC TGGTTAAATT TGTGGACATT 
CTAACCAACT GGTATGTCAG AATGAATCGA CGGAGGTTAA AGGGTGAGAA TGGAACAGAA 
GACTGCATTA TGGCCTTAGA AACCTTATTC AGCGTCCTCT ACTCCATGTG CAGACTTATG 
GCACCTTATA CTCCGTTTAT TACCGAACTA ATGTACCAGA ATTTGAAGAC TCTTATTCAT 
CCTGCTTGTA TTCAGGAGAA GAATACTGAT AGCATTCACT ACCTCATGCT TCCTCAAGTT 
AGGGAAGACC TGATCGACAA AAACATAGAA AGTGCTGTTT CTTGGATGCA GTCTGTTATT 
GAGCTGGGAC GTGTAATCAG AGATAGGAAA ACCATCCCAG TAAAGTATCC ATTGAAAGAA 
GTGGTCATCA TCCATCAGGA TTCTCAAGCA CTGGAAGACA TCAAGTCCTT GGAAAAGTAT 
ATACTTGAGG AACTTAATGT CCGCAAAGTG ACTTTATCCA CTGATAAAGA TAAGTATGGA 
ATCCGTTTGA GAGCAGAACC TGATCATATG GTGCTTGGGA AGCGACTTAA AGGTGCCTTT 
AAATCTGTAA TGGCTGCTAT CAAAGAATTG CCAAGTGAAC TGTTGGAAGA ATTTCAGAAG 
ACAGGCAAAA TGGTTGTAGA AGGGCATGAA TTGCATGAGG AAGATCTCCG TCTGATGTAC 
ACCTTTGACC AGACTGGAGG AGGATATGGC CAGTTTGAAG CTCATTCTGA TTCTCAGGTT 
TTGGTTCTCC TAGATATTAC TCCTGATCAG TCTATGGTTG ATGAAGGCGT TGCACGGGAA 
GTAATAAATC GCATTCAAAA ACTTCGAAAG AAGCGCAATC TGGTTCCTAC AGATGAGATT 
ACAGTATATT ATCAGGCATC TCCAGAAGGT GATTATCTTG ATACTGTTAT TAAGGAACAT 
ACAGATTTTA TTTTTGCCAC AATAAAAACT GCTTTGAAGC CATATCCAGT GTCTCCCTCA 
GAGGAAGTGC TTATTCAAGA AAAAACTCAG CTGAAGGGAT CAGAACTGGA AATTACAATA 
GCCAAAGCAG CTGTTCATCA CTGTACTGAA CCAGCATGTG CTTATGTCAC TCTGAACATC 
TGCATTAATG GTAAAGAACA AGATGGTATG GTGTTGCTGG AAAATCCGAA GGGAGACAAT 
AAATTAGACT TCACCAAATT TGTTAATACT ATTGCGTGCA TTTTTGGCCT TGAAAAAGCA 
AAAGTAGCAA TTTATAGTGG AAAACAAGAA GTGAAAAAAC AAACAGATTT GCTGAGTCTT 
AATGGAAAGA AGCTTCATGT AACTGCAGGG CCATTGCCAA TTACAAACAG TATTGATGAC 
TTTTTATGTC AGTATATCAA TTTACAACTA GTGAATGCAA AACCACAAGA GTGTCTAAAT 
GGAACGGTGG GAACCCTTCT TACGGAAAAC CCTGTTGGTC AAAATGGATT AACATATCAA 
GGGCTTCTGC ATGAAACAGG CAAAATGTTC GGACTCAGAA GCAGGAGGCT AAAATTGTTC 
TTGGATGAAG CGCTGACAAA AGAGGTTACA AAGGAGACTT CCATGAAGTT CTTAAATACA 
AAGACTCTGT ATGTTCATGC TATACCAACA ACAGCTGAAT GCTAA