AKAP1 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	AKAP1
Entrez Gene ID	106539376
Full Name	A-kinase anchoring protein 1
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014054162.1	XP_013909637.1	A-kinase anchor protein 1, mitochondrial

Pathways from BioSystems
Gene Ontology

The following AKAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AKAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh15357	XM_014054162.1 Latest version!	Thamnophis sirtalis A kinase (PRKA) anchor protein 1 (AKAP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AKAP1 ( XM_014054162.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013909637.1 Thamnophis sirtalis A kinase (PRKA) anchor protein 1 (AKAP1), mRNA.

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CloneID	OTh15357
Clone ID Related Accession (Same CDS sequence)	XM_014054162.1
Accession Version	XM_014054162.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2529bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	A-kinase anchor protein 1, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013657902.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 7% of CDS bases ##RefSeq-Attributes-END##

ATGGCGTTGC GCTTCCGCAA TTTCCTGCCC TTCACCATCC CCGGAGTGCT GGCGCTCATC 
GGCTGGTGGT GGTTTTCTTC ACGTAAAAAG GAGTGCGTCA GTAACCACGA CAGGCAAGAT 
CTTCCCAGCT TGGAAGAACG CCAGAAATGT TCTCCTCCGA AGGAGCCAAG TTCTTGGAAA 
ACTGGACAGT TGGACAGTGA ACTTTCTCCA CCTCTACAGG TCAGGCAGTG TAGCGATTCA 
CCCTTTCAAG AACGACCGAC ACCTTTAACA GAAGACAGCG CCCAAAGCTT AGAAACGTTG 
AGAAAAGACC TTCCCGTTGG GGCAACACTA ACCCTCCAAC CGGATTCACT TCAGGACGAC 
AGTGGAGAAG TGGAAGTGAT GGGGCCTCAA GGTGTCCTTG GTTCCCCTTC GCCCAAGATC 
TCTCTTCTGG ATGTACAAAG AGTTGATGAC TCCCAAATGG ATAAGACAGT CAATGAAGGG 
AAGCCCGATT CCAATCAAGC ATCCAACCAA GGGCTGTTGG CAACTCCTGG TTCTGAAAAA 
TGGGAGGACA ATAGCAAAGA TGGGATGGTC CAGATCGGTT CATCCGAGAG GCCTTTGAGA 
GAAGACCAGT ATTCTGCATC CACCTCTATG GATTCTTTCC CCGTCGCCTG CTCCAGAAAG 
ATAACTGAAG AAGTTCCTGA TGAGATGCCT AAGGAACGCG TTGAAACGGA AGAAGGAAGC 
GATAGTAACA GTGGCGAAGG AGAGGCTGCA GTTGGGACAC TGACCAAGTG TGTTGAACCT 
GCTAGGTCGG AGATGGTTGA GGAAGAGATG ACGTCCAGTT CAAAGACTGG TAGCATGTCA 
AAGCACAATG GATGCAGAGA AGTTGGGTAT AAGGGTGGTC TGGAAAAAGA GAGGATAGGA 
GCCATTAGTT TGGACAAACA AGAAGTGGAG AAGATCGAGC AGGTGGCCAT TCAAATCATT 
TCCAATGTCA TCCGGGCAGC CACTGAGGAA GTATTATCAG GGTCGGTCAG TGATATGTCA 
GAGAGGATAT GCCAAATAGC AAGCCGGGTG GATAGTCCTC GGGAGAAGGT GTGCACTGGA 
GTTGTTAGCC AAGCAGAATC CAGCCAGGGT GGTGACTGTG TGAGCCAGAA GGTTGCTACT 
GTGGAAGTAT CTCCCCTCCT GGAGGAGAAC GTCAACCGTC ATGCTACCTA TTCAAGTTGT 
TTGACTCACG GCCAACTGGT CAACCGAAGT CACAACCGGC TCAGAGACTC CAGTTGTCCC 
ACCAGGCGGT CTGTTGGCGG CCTGACACCA GCTACGAACC ACGAGGAGGA GTTTGAGGAT 
GGTCACATAG TGATGGAAGA CTCTGGGTGC AGCGCCTGCA CCTCGGAAGA TGGGGCTAGT 
GCCGAGGATC TCTTGAAAAG TGCTTCATCT CCACCTCCAC AACACCTGGA TCTGTTGAAT 
GCCTCCACCA TCAAAGACTC AGAGAACAAG TCCGCGTCGA TCCAGAAGCC ACCTTCCTCC 
ATGTCAACGG AAAACAGGGT GCCATACAGC AATGGCGTGC TGAAGGAGGA CTTTCCGCCT 
CTGAGCCACG AACAGCCATG GCCCACGGAG GCCGATACAG ACCACTCAGG AGGCTCTGAC 
GTGAACAGCA TGGACTCGGT GGACAGCGGC TGTGCTCTCC GCAAGAATGA CAGCTGCCTG 
ACTGCCAAGA TGGGAACGGA CTGCAATAAG GCAGAGCTTG TCATCTGGGA AATTGAAATA 
CCGAAGCACT TAGTAGGACG GCTGATAGGC AAACAAGGGA GATACGTGAG TTACCTGAAG 
CAGGCCTCCG GTGCCAAAAT CTACATTTCC ACTCTACCTT ACACCCAAGA CTTCCAGATC 
TGCCATATAG AAGGCTCTCA GCAAAACGTT GACAAAGCAC TGACCCTGAT TGGAAAGAAG 
TTCAAGGATT TGAGTCTCAC CAACATCTAC GCTCCCCCAC CCCCACCGTT GCCATTGCAT 
TCCCTCCCCA TGACCTCCTG GGGCTTCATT GAACAAATGA TGCTAGTTGG AATGGAGTCC 
ATGGACATTG GCATCCATTT GATGAAATGT TGGCCACCTC TCTCCAGGTT CCATTTGGGG 
CAGAGAATGT CTCATCACCT GCAAGTTGGC GTTGTCTGTG CTGCGCCGGG TGTGGAAGGC 
GCATGGTGGC GGGCACAAGT GATTAGCTAC TTCAAAGATG TCAGCGAAGT GGAAATCAGA 
TACGTGGATT ATGGTGGATA CGAACGAGTC AAGATTGACA CGCTTAGACA AATCAGGTCT 
GACTTTGTAA CCCTGCCGTT CCAAGGAGCT GAGGTTCTTT TGGACAACGT GGTGCCACTT 
CCAGGTAATA ACCCCCAGAA ACCTAAGAGA GATGTTTTTA TCTTTGTGGT TTTATTACAG 
GTTACAAATT ACGACAGTGC AACGGGCTTG CCACTAATAC AACTGTGGAG CATGATAGGA 
GATGAGTTGG TGTCCATCAA CCGAACACTG GTGGAACGAG GATTTGCTCA GTGGGTGGAA 
AGCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013909637.1
CDS	21..2549
Translation	MALRFRNFLPFTIPGVLALIGWWWFSSRKKECVSNHDRQDLPSLEERQKCSPPKEPSSWKTGQLDSELSPPLQVRQCSDSPFQERPTPLTEDSAQSLETLRKDLPVGATLTLQPDSLQDDSGEVEVMGPQGVLGSPSPKISLLDVQRVDDSQMDKTVNEGKPDSNQASNQGLLATPGSEKWEDNSKDGMVQIGSSERPLREDQYSASTSMDSFPVACSRKITEEVPDEMPKERVETEEGSDSNSGEGEAAVGTLTKCVEPARSEMVEEEMTSSSKTGSMSKHNGCREVGYKGGLEKERIGAISLDKQEVEKIEQVAIQIISNVIRAATEEVLSGSVSDMSERICQIASRVDSPREKVCTGVVSQAESSQGGDCVSQKVATVEVSPLLEENVNRHATYSSCLTHGQLVNRSHNRLRDSSCPTRRSVGGLTPATNHEEEFEDGHIVMEDSGCSACTSEDGASAEDLLKSASSPPPQHLDLLNASTIKDSENKSASIQKPPSSMSTENRVPYSNGVLKEDFPPLSHEQPWPTEADTDHSGGSDVNSMDSVDSGCALRKNDSCLTAKMGTDCNKAELVIWEIEIPKHLVGRLIGKQGRYVSYLKQASGAKIYISTLPYTQDFQICHIEGSQQNVDKALTLIGKKFKDLSLTNIYAPPPPPLPLHSLPMTSWGFIEQMMLVGMESMDIGIHLMKCWPPLSRFHLGQRMSHHLQVGVVCAAPGVEGAWWRAQVISYFKDVSEVEIRYVDYGGYERVKIDTLRQIRSDFVTLPFQGAEVLLDNVVPLPGNNPQKPKRDVFIFVVLLQVTNYDSATGLPLIQLWSMIGDELVSINRTLVERGFAQWVESY

Target ORF information:

RefSeq Version	XM_014054162.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis A kinase (PRKA) anchor protein 1 (AKAP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014054162.1

ORF Insert Sequence:

ATGGCGTTGC GCTTCCGCAA TTTCCTGCCC TTCACCATCC CCGGAGTGCT GGCGCTCATC 
GGCTGGTGGT GGTTTTCTTC ACGTAAAAAG GAGTGCGTCA GTAACCACGA CAGGCAAGAT 
CTTCCCAGCT TGGAAGAACG CCAGAAATGT TCTCCTCCGA AGGAGCCAAG TTCTTGGAAA 
ACTGGACAGT TGGACAGTGA ACTTTCTCCA CCTCTACAGG TCAGGCAGTG TAGCGATTCA 
CCCTTTCAAG AACGACCGAC ACCTTTAACA GAAGACAGCG CCCAAAGCTT AGAAACGTTG 
AGAAAAGACC TTCCCGTTGG GGCAACACTA ACCCTCCAAC CGGATTCACT TCAGGACGAC 
AGTGGAGAAG TGGAAGTGAT GGGGCCTCAA GGTGTCCTTG GTTCCCCTTC GCCCAAGATC 
TCTCTTCTGG ATGTACAAAG AGTTGATGAC TCCCAAATGG ATAAGACAGT CAATGAAGGG 
AAGCCCGATT CCAATCAAGC ATCCAACCAA GGGCTGTTGG CAACTCCTGG TTCTGAAAAA 
TGGGAGGACA ATAGCAAAGA TGGGATGGTC CAGATCGGTT CATCCGAGAG GCCTTTGAGA 
GAAGACCAGT ATTCTGCATC CACCTCTATG GATTCTTTCC CCGTCGCCTG CTCCAGAAAG 
ATAACTGAAG AAGTTCCTGA TGAGATGCCT AAGGAACGCG TTGAAACGGA AGAAGGAAGC 
GATAGTAACA GTGGCGAAGG AGAGGCTGCA GTTGGGACAC TGACCAAGTG TGTTGAACCT 
GCTAGGTCGG AGATGGTTGA GGAAGAGATG ACGTCCAGTT CAAAGACTGG TAGCATGTCA 
AAGCACAATG GATGCAGAGA AGTTGGGTAT AAGGGTGGTC TGGAAAAAGA GAGGATAGGA 
GCCATTAGTT TGGACAAACA AGAAGTGGAG AAGATCGAGC AGGTGGCCAT TCAAATCATT 
TCCAATGTCA TCCGGGCAGC CACTGAGGAA GTATTATCAG GGTCGGTCAG TGATATGTCA 
GAGAGGATAT GCCAAATAGC AAGCCGGGTG GATAGTCCTC GGGAGAAGGT GTGCACTGGA 
GTTGTTAGCC AAGCAGAATC CAGCCAGGGT GGTGACTGTG TGAGCCAGAA GGTTGCTACT 
GTGGAAGTAT CTCCCCTCCT GGAGGAGAAC GTCAACCGTC ATGCTACCTA TTCAAGTTGT 
TTGACTCACG GCCAACTGGT CAACCGAAGT CACAACCGGC TCAGAGACTC CAGTTGTCCC 
ACCAGGCGGT CTGTTGGCGG CCTGACACCA GCTACGAACC ACGAGGAGGA GTTTGAGGAT 
GGTCACATAG TGATGGAAGA CTCTGGGTGC AGCGCCTGCA CCTCGGAAGA TGGGGCTAGT 
GCCGAGGATC TCTTGAAAAG TGCTTCATCT CCACCTCCAC AACACCTGGA TCTGTTGAAT 
GCCTCCACCA TCAAAGACTC AGAGAACAAG TCCGCGTCGA TCCAGAAGCC ACCTTCCTCC 
ATGTCAACGG AAAACAGGGT GCCATACAGC AATGGCGTGC TGAAGGAGGA CTTTCCGCCT 
CTGAGCCACG AACAGCCATG GCCCACGGAG GCCGATACAG ACCACTCAGG AGGCTCTGAC 
GTGAACAGCA TGGACTCGGT GGACAGCGGC TGTGCTCTCC GCAAGAATGA CAGCTGCCTG 
ACTGCCAAGA TGGGAACGGA CTGCAATAAG GCAGAGCTTG TCATCTGGGA AATTGAAATA 
CCGAAGCACT TAGTAGGACG GCTGATAGGC AAACAAGGGA GATACGTGAG TTACCTGAAG 
CAGGCCTCCG GTGCCAAAAT CTACATTTCC ACTCTACCTT ACACCCAAGA CTTCCAGATC 
TGCCATATAG AAGGCTCTCA GCAAAACGTT GACAAAGCAC TGACCCTGAT TGGAAAGAAG 
TTCAAGGATT TGAGTCTCAC CAACATCTAC GCTCCCCCAC CCCCACCGTT GCCATTGCAT 
TCCCTCCCCA TGACCTCCTG GGGCTTCATT GAACAAATGA TGCTAGTTGG AATGGAGTCC 
ATGGACATTG GCATCCATTT GATGAAATGT TGGCCACCTC TCTCCAGGTT CCATTTGGGG 
CAGAGAATGT CTCATCACCT GCAAGTTGGC GTTGTCTGTG CTGCGCCGGG TGTGGAAGGC 
GCATGGTGGC GGGCACAAGT GATTAGCTAC TTCAAAGATG TCAGCGAAGT GGAAATCAGA 
TACGTGGATT ATGGTGGATA CGAACGAGTC AAGATTGACA CGCTTAGACA AATCAGGTCT 
GACTTTGTAA CCCTGCCGTT CCAAGGAGCT GAGGTTCTTT TGGACAACGT GGTGCCACTT 
CCAGGTAATA ACCCCCAGAA ACCTAAGAGA GATGTTTTTA TCTTTGTGGT TTTATTACAG 
GTTACAAATT ACGACAGTGC AACGGGCTTG CCACTAATAC AACTGTGGAG CATGATAGGA 
GATGAGTTGG TGTCCATCAA CCGAACACTG GTGGAACGAG GATTTGCTCA GTGGGTGGAA 
AGCTACTAG