GFRA1 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	GFRA1
Entrez Gene ID	106538510
Full Name	GDNF family receptor alpha 1
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014053008.1	XP_013908483.1	GDNF family receptor alpha-1

Pathways from BioSystems
Gene Ontology

The following GFRA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GFRA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh14473	XM_014053008.1 Latest version!	Thamnophis sirtalis GDNF family receptor alpha 1 (GFRA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GFRA1 ( XM_014053008.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013908483.1 Thamnophis sirtalis GDNF family receptor alpha 1 (GFRA1), mRNA.

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CloneID	OTh14473
Clone ID Related Accession (Same CDS sequence)	XM_014053008.1
Accession Version	XM_014053008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	GDNF family receptor alpha-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013657848.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCTGG CTACCTTCTA CTTCGTGTTG CCCTTAATGG AAGTGCTCTT CTCGGCTGAA 
GTGAGTGGGC TGCTTGGAGG AGACCATCTG GATTGTGTGA AAGCCAGCGA CCAGTGTCTC 
AGGGAGCAGA GCTGCAGCAC TAAATACAGG ACACTGAGGC AGTGTGTAGC TGGCAAAGAG 
AGCAACTTCA GCCGGGCAGC AGGCCTGGAG GCAAAGGACG AGTGCAAAAG CGCCATGGAG 
GCTCTCAAGC AGAAATCCCT CTATAATTGC CGCTGCAAAA GAGGCATGAA AAAGGAGAAA 
AACTGTCTGC GTATTTACTG GAGCATGTAC CAAAGCTTAC AGGGAAATGA TTTGCTTGAA 
GAATCACCTT ACGAACCAGT GAACAGCAGG TTAACAGACA TATTCAGGCT AGCACCAATT 
GTATCAGCGG AGCCTGTCAT TTCAAAAGGG AACAACTGTC TGGATGCTGC AAAAGCCTGT 
AACCTGAATG ATATCTGCAA GAAATATCGA TCGGCGTACA TAACACCCTG TACCTACAGT 
GTGTCTAATG AAATCTGCAA CAAGCGGAAG TGTCACAAGG CCCTAAGGCA GTTTTTTGAC 
AAAGTTCCCC CCAAGCATAG TTATGGAATG CTCTTCTGTT CCTGCCGAGA TATTGCATGT 
ACAGAAAGAA GACGACAGAC TATTGTCCCT GCGTGTTCTT ATGAGGACAG GGAGAAACCA 
AACTGCCTGA ATTTGCAAGA GACTTGCAAG ACGAATTACA TCTGCAGATC TCGTCTTGCA 
GACTTTTTCA CAAACTGCCA GCCTGAGTCA CGGTCTGTTA GTAACTGCCT GAAAGAAAAC 
TATGCTGACT GTCTCCTTGC TTACTCAGGG CTTATTGGCA CAGTTATGAC ACCAAATTAT 
TTAAATTATA ATAATCTCAG CGTTGCACCT TGGTGTGATT GCGGCAGCAG TGGCAATGAC 
ATAGAAGAAT GTCTGAAATT TCTGAATTTC TTCCAGGATA ATTCATGCCT TAAAAGTGCA 
ATTCAGGCTT TTGGCAATGG TACAGATGTG AATGTGTGGC AACCGTTCCT ACCTGGCCAA 
ACCACCACAG CTTTAACTAC AACAGCATAC AGATCAAAGG CCTATGACTC TGAGAATGCT 
AACAATGAAA TACCCACTAA TAATGATTTG CCAGCCTGTG CCAACATCAA GGGACCAAAG 
CAGAGTTTCA ATGAATCTAT AATAACAGAG CCCTGTGTGA CTGGGAATGC AATTGGGAAG 
GAAAGTGTGG CAGGAGGCTC CACCAGTCAC ATATCTTCTG ATCATTCGTC TTCTCTTCGT 
GTAAGCGTCA GGTCAAGCAC ACTTCTTTTG CTGTTGGTGA TTGCACTCCC ATTCTGTTGG 
TGCCCACGTT CAGAAGAAAC CTTGTATCTG CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013908483.1
CDS	354..1769
Translation	MPLATFYFVLPLMEVLFSAEVSGLLGGDHLDCVKASDQCLREQSCSTKYRTLRQCVAGKESNFSRAAGLEAKDECKSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEESPYEPVNSRLTDIFRLAPIVSAEPVISKGNNCLDAAKACNLNDICKKYRSAYITPCTYSVSNEICNKRKCHKALRQFFDKVPPKHSYGMLFCSCRDIACTERRRQTIVPACSYEDREKPNCLNLQETCKTNYICRSRLADFFTNCQPESRSVSNCLKENYADCLLAYSGLIGTVMTPNYLNYNNLSVAPWCDCGSSGNDIEECLKFLNFFQDNSCLKSAIQAFGNGTDVNVWQPFLPGQTTTALTTTAYRSKAYDSENANNEIPTNNDLPACANIKGPKQSFNESIITEPCVTGNAIGKESVAGGSTSHISSDHSSSLRVSVRSSTLLLLLVIALPFCWCPRSEETLYLL

Target ORF information:

RefSeq Version	XM_014053008.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis GDNF family receptor alpha 1 (GFRA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014053008.1

ORF Insert Sequence:

ATGCCTCTGG CTACCTTCTA CTTCGTGTTG CCCTTAATGG AAGTGCTCTT CTCGGCTGAA 
GTGAGTGGGC TGCTTGGAGG AGACCATCTG GATTGTGTGA AAGCCAGCGA CCAGTGTCTC 
AGGGAGCAGA GCTGCAGCAC TAAATACAGG ACACTGAGGC AGTGTGTAGC TGGCAAAGAG 
AGCAACTTCA GCCGGGCAGC AGGCCTGGAG GCAAAGGACG AGTGCAAAAG CGCCATGGAG 
GCTCTCAAGC AGAAATCCCT CTATAATTGC CGCTGCAAAA GAGGCATGAA AAAGGAGAAA 
AACTGTCTGC GTATTTACTG GAGCATGTAC CAAAGCTTAC AGGGAAATGA TTTGCTTGAA 
GAATCACCTT ACGAACCAGT GAACAGCAGG TTAACAGACA TATTCAGGCT AGCACCAATT 
GTATCAGCGG AGCCTGTCAT TTCAAAAGGG AACAACTGTC TGGATGCTGC AAAAGCCTGT 
AACCTGAATG ATATCTGCAA GAAATATCGA TCGGCGTACA TAACACCCTG TACCTACAGT 
GTGTCTAATG AAATCTGCAA CAAGCGGAAG TGTCACAAGG CCCTAAGGCA GTTTTTTGAC 
AAAGTTCCCC CCAAGCATAG TTATGGAATG CTCTTCTGTT CCTGCCGAGA TATTGCATGT 
ACAGAAAGAA GACGACAGAC TATTGTCCCT GCGTGTTCTT ATGAGGACAG GGAGAAACCA 
AACTGCCTGA ATTTGCAAGA GACTTGCAAG ACGAATTACA TCTGCAGATC TCGTCTTGCA 
GACTTTTTCA CAAACTGCCA GCCTGAGTCA CGGTCTGTTA GTAACTGCCT GAAAGAAAAC 
TATGCTGACT GTCTCCTTGC TTACTCAGGG CTTATTGGCA CAGTTATGAC ACCAAATTAT 
TTAAATTATA ATAATCTCAG CGTTGCACCT TGGTGTGATT GCGGCAGCAG TGGCAATGAC 
ATAGAAGAAT GTCTGAAATT TCTGAATTTC TTCCAGGATA ATTCATGCCT TAAAAGTGCA 
ATTCAGGCTT TTGGCAATGG TACAGATGTG AATGTGTGGC AACCGTTCCT ACCTGGCCAA 
ACCACCACAG CTTTAACTAC AACAGCATAC AGATCAAAGG CCTATGACTC TGAGAATGCT 
AACAATGAAA TACCCACTAA TAATGATTTG CCAGCCTGTG CCAACATCAA GGGACCAAAG 
CAGAGTTTCA ATGAATCTAT AATAACAGAG CCCTGTGTGA CTGGGAATGC AATTGGGAAG 
GAAAGTGTGG CAGGAGGCTC CACCAGTCAC ATATCTTCTG ATCATTCGTC TTCTCTTCGT 
GTAAGCGTCA GGTCAAGCAC ACTTCTTTTG CTGTTGGTGA TTGCACTCCC ATTCTGTTGG 
TGCCCACGTT CAGAAGAAAC CTTGTATCTG CTTTAA