TTHERM_00410250 cDNA ORF clone, Tetrahymena thermophila SB210

Gene
Clones
gRNAs

Summary

Gene Symbol	TTHERM_00410250
Entrez Gene ID	7836062
Full Name	vacuolar sorting protein, putative
Gene Type	protein-coding
Organism	Tetrahymena thermophila SB210

mRNA and Protein(s)

mRNA	Protein	Name
XM_001020818.1	XP_001020818.1	vacuolar sorting protein, putative

Pathways from BioSystems
Gene Ontology

The following TTHERM_00410250 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTHERM_00410250 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTe52644	XM_001020818.1 Latest version!	Tetrahymena thermophila SB210 vacuolar sorting protein, putative partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTHERM_00410250 ( XM_001020818.1 ) cDNA ORF clone, Tetrahymena thermophila SB210 -> XP_001020818.1 Tetrahymena thermophila SB210 vacuolar sorting protein, putative partial mRNA.

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CloneID	OTe52644
Clone ID Related Accession (Same CDS sequence)	XM_001020818.1
Accession Version	XM_001020818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-08
Organism	Tetrahymena thermophila SB210
Product	vacuolar sorting protein, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002476326). COMPLETENESS: incomplete on both ends.

ATGGATTAGG ATTCTGAAGG TTTTTAAGAA AGGATTGATT TGCCAGATCC ATTTGAAAAA 
AAGAAAATGG ATTTTGGCCT ACCTGATTAT GAAAGCATCA AGCAACTGTA AATTACAGAA 
AAGTAGTTAG TCATTTTAAC AGAAACAGGG AAAATTTTTA GATAATTATA TGAAGAAAGA 
ATTTAATTGC AAGAAATAGA ATTGCCATAA TCATCATAGC CTATTTATAA TATTGTTACT 
AACATTTTTG GAAAAAGCTA ATCATATGAG CCTATAGAAA AATTTTTTTT AGATAAGACT 
GGAAATCATT GCTTACTGCA AGGAAAAAAT GAGTAAACAT ACTATTGGCA TGCATCTGAA 
AACAAGATTA AGATAGTTTT GTCTAAATTC AATAAAGATA TAACATCTTT GGCATTTGAT 
GATGCAGCAA TCTCTGATCA AGAAGTTGCT TTCTTATTTG GAACAAAAAG CAAAGAATTT 
TTTTATGGTA GAATACAAAG GAGAAAGACA AATGACATAG AGTTTACTTG CTATTAAATT 
GATTCGCTAG TAACGAAGAG CCCTATTTAA AAGATTTTCA TTTATCAGCT AGATTATTAA 
AAAACCAATT ACACATGTGT ACTTGTATGT GATGATTAGA CCTTTAATTA CTTCTATGAT 
GAGCAGCCTT TGCCAAATCT TTTTAAAAAG TATGAAAAAG GAAGTGAAAA TCTTGATGAA 
ATACCAATCA ATCCTCCAGG AAATATAATT ATACATATGG CTAAAAAAAT TGGTTGTAAT 
AGACCTCATT CTTTTGCTTA TACCACAGGT TCTACTATAA TATGTCTATA ATTATCATCT 
GTTAATGAAT TACCCAACGA CTCTTTCTTG AAAAACTTTA AATTTCTTTA GTATGCTAAA 
ACAGAAGAAG ATTCTCGTAA AGATGTGAAA GATAGATTTT TAATTGAAGA AAGAATCCTC 
AAAATAGGAT TAACAGAATT TCATTACTTT TTGCTTCATG AGGACTGTCT AACAGTAATG 
AGTAGATTAG ATTAAAAGGT AGTTACTACA TTTGAACTGG GAAATATGTA GGGACCTGTG 
ATTGATATGA TCTACGATGA TTCTTCCAGT TAAAGATCTT TTCTTATTTA TTCTAGAAAG 
AGAATTTTAA AAATTGTGAT TAACATGGAA GAAGAATTTG CATGCTATGA TTTAATTAAG 
TAAAAAGATA TAAAAGGTGC TTACGAGCTA TGCAAGAGAA CAAATTTGGA CTGTCTTCCT 
TATGTTTCTG GTTTATATGG AGATTATTTG TATTAGGAAG GAAAGTATGT GACTGCTGCA 
TAAGTTTATT CAACATCATA AAAAACATTC GAAGAAATTA TGATTAAGTT CTTAAAAAAC 
GAATCACTTG AATAGATTGA AGGCATAATA GAGTATCTGT AAATTTAAGC TGCAAGACTC 
AATAATTAGA AAAATTAGGT TTAATTTAAA ATAATTTCAG GATTGAGCTT AGAATATTAC 
AACTATAAAA TTAATGAATT AGAGAAGTAT TTAGAAGTGC TTATTTAAGA GGAGGCATAA 
AAAGAATTAG AAAAGTAATC AGACTAACAT CTTAAATTTA AAATAGAATA TAGAGGCAAT 
TAGCTACAGG TAGTTTATTT AGATAATTTA AATGAAATGA GATTGAGAAA GCAAAGTGGT 
GAAGATCAAA CACCAATGTC TTAGCCCTCA AATAAATTGA GTAACGATGG TTAAGCTGAT 
TAAAAAAGAA AAGCTTCTTA AAATTCTATC ACAAGTAAAA GAATTGTCAG GATATAAAGC 
AAGCTTAAAA AAATAAAAGA TAATTATAAC TCATTTTTGA AAAATCTTAA TTCTTAAATA 
GATCAAGAAT TCGTTTATGA AATAACTCAA AGTCATGGAA GAATTGATGA CTGCTTACAG 
TATGCAAAGG AGTAAAATAA TTATGAAAAT GTTATAACAC ATTATGTAAA CGAATAAAAT 
TACAAAGAAG CTGTCAGCTA TCTAAAAGTT TTAGCCAAAA AAGACAGCAA AAGAGCCATA 
GAAATCATAT ACAAATTTTC TTACATTTTA CTCTAAAAAG TTCCTGAAGA ATTCATTTCT 
TTGGTCTTAA ACTCTATCAG AGATTTCCAA CCTTCTAAAT TGATATCAGG ATTAATGAAT 
ATTCCAGGCA CCTTTAGAAA ATTTGGAATC TAATTTTTAG AAAATTGTGT TTATAAATAA 
AAGAGCTCTG ATAAAATCAT TCACAATCTT TATCTTTTTT TCTTGGCAAA CCAAATTCGT 
CCTGATGAAA ACAGCCAAAC TTAAAAAGTT GATGAGGAAG CTTACTAATA ACTTATGGAT 
TACTTGAACA AAGCAGAAGA ACAAGAAAAC TAGTAAATTT ATGGAAGTTT ATAGAATGAT 
GATTCTGAAA TAAGTTTGTA TTCATAAAAA TCATCAAGAA ATTCTCAAAA GCAACAAAAT 
TATATTCTGA AATTTGATTT AGATTTTGCA CTTCATTTAT TTGAATCAAA AATGATTACA 
GATGCGATGG TCAAGACCTA TGCTTTGATG AATTTTTATG ATTTAGCAGT TAAATTAGCT 
TTAGATAAAA ATAACATTAG CCTTGCTAAA GAATATGCTT CAAAGCACCA TAATGATGAT 
AAAAAACATA AATTGTGGCT CAAAATAGCA GTTCATTTAA TTTAAACATA GTAATCCCAA 
AATGTTCTAT AATTAACAAA TGACAGTAAT GGTTATTTAA AAGTAGAGGA TCTTCTTCCA 
CATTTTGGAG AAGTTCATCA AATAGACCTA CTCAAAGAAT AAATTTGCAG TAATTTAGAA 
GACTATAACA ATGAGATTGA AAATTTGAAG AAAGAAATGG ATTAACTAAA CGAAATGTCA 
GATTCACTTA AGCGTGAAAT TAATACTTAT TCTCATAAAT CTCTATAGAT TAAGGGAGAA 
TAGGATTGCA GTATATGTCA CAGAAACCTT CTTTCATAAG ATCATTATTT CTTTGGCTGC 
TTCCATGGTT TTCATAGAAA TTGCTACATT TAAAGAATGT AAAGTGATAT AATAGCTGAA 
TAAGAGAAAG AATAGATAAG CGATTAAGGC GAAAAATAAC TAACTTCTTA TTCTCAAAAG 
AGAGTTTTTG ATAAAATAAT ATCAATAAAT TAGTACATCA ATAAAATCTA ATCGGATTTA 
AAAAATAAAC AAGAAGAAGA AATTAAGTAA ATGAAATTAA TGTTAATAGA TGAAAATCAA 
TAATAGTAAT CCTCTTCAAT TACTAAATTC TTTAAAGGAC TTGGAGGTGC TTTAATAGGT 
GGAGCAGGTA AAAAAGAAGA TGAATAATAA GCTACTATAG ATATTAATGC TTTCAAAAAA 
AAACTAAGTT CTATGGCAAA TCTCAAAGAT ATGTTATAAT TATTTTTAAG TTAAGAAGAA 
AGGGATGAAT ATTATAAACA AGTATAAATT GTTGATGACT TTCTCTCAAA AGAGTGTTAT 
TTTTGTGGAC TTTCTTCTGT TGATCGTATT CATACATTTT ATCCTAAAAA TACTAAGAGT 
TCTATTGATT GGGCGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001020818.1
CDS	1..3561
Translation	MDDSEGFERIDLPDPFEKKKMDFGLPDYESIKQLITEKLVILTETGKIFRLYEERILQEIELPSSPIYNIVTNIFGKSSYEPIEKFFLDKTGNHCLLQGKNETYYWHASENKIKIVLSKFNKDITSLAFDDAAISDQEVAFLFGTKSKEFFYGRIQRRKTNDIEFTCYIDSLVTKSPIKIFIYQLDYKTNYTCVLVCDDTFNYFYDEQPLPNLFKKYEKGSENLDEIPINPPGNIIIHMAKKIGCNRPHSFAYTTGSTIICLLSSVNELPNDSFLKNFKFLYAKTEEDSRKDVKDRFLIEERILKIGLTEFHYFLLHEDCLTVMSRLDKVVTTFELGNMGPVIDMIYDDSSSRSFLIYSRKRILKIVINMEEEFACYDLIKKDIKGAYELCKRTNLDCLPYVSGLYGDYLYEGKYVTAAVYSTSKTFEEIMIKFLKNESLEIEGIIEYLIAARLNNKNVFKIISGLSLEYYNYKINELEKYLEVLIEEAKELEKSDHLKFKIEYRGNLQVVYLDNLNEMRLRKQSGEDQTPMSPSNKLSNDGADKRKASNSITSKRIVRISKLKKIKDNYNSFLKNLNSIDQEFVYEITQSHGRIDDCLQYAKENNYENVITHYVNENYKEAVSYLKVLAKKDSKRAIEIIYKFSYILLKVPEEFISLVLNSIRDFQPSKLISGLMNIPGTFRKFGIFLENCVYKKSSDKIIHNLYLFFLANQIRPDENSQTKVDEEAYLMDYLNKAEEQENIYGSLNDDSEISLYSKSSRNSQKQQNYILKFDLDFALHLFESKMITDAMVKTYALMNFYDLAVKLALDKNNISLAKEYASKHHNDDKKHKLWLKIAVHLITSQNVLLTNDSNGYLKVEDLLPHFGEVHQIDLLKEICSNLEDYNNEIENLKKEMDLNEMSDSLKREINTYSHKSLIKGEDCSICHRNLLSDHYFFGCFHGFHRNCYIRMSDIIAEEKEISDGEKLTSYSQKRVFDKIISINYINKISDLKNKQEEEIKMKLMLIDENQ*SSSITKFFKGLGGALIGGAGKKEDEATIDINAFKKKLSSMANLKDMLLFLSEERDEYYKQV*IVDDFLSKECYFCGLSSVDRIHTFYPKNTKSSIDWAI

Target ORF information:

RefSeq Version	XM_001020818.1
Organism	Tetrahymena thermophila SB210
Definition	Tetrahymena thermophila SB210 vacuolar sorting protein, putative partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001020818.1

ORF Insert Sequence:

ATGGATTAGG ATTCTGAAGG TTTTTAAGAA AGGATTGATT TGCCAGATCC ATTTGAAAAA 
AAGAAAATGG ATTTTGGCCT ACCTGATTAT GAAAGCATCA AGCAACTGTA AATTACAGAA 
AAGTAGTTAG TCATTTTAAC AGAAACAGGG AAAATTTTTA GATAATTATA TGAAGAAAGA 
ATTTAATTGC AAGAAATAGA ATTGCCATAA TCATCATAGC CTATTTATAA TATTGTTACT 
AACATTTTTG GAAAAAGCTA ATCATATGAG CCTATAGAAA AATTTTTTTT AGATAAGACT 
GGAAATCATT GCTTACTGCA AGGAAAAAAT GAGTAAACAT ACTATTGGCA TGCATCTGAA 
AACAAGATTA AGATAGTTTT GTCTAAATTC AATAAAGATA TAACATCTTT GGCATTTGAT 
GATGCAGCAA TCTCTGATCA AGAAGTTGCT TTCTTATTTG GAACAAAAAG CAAAGAATTT 
TTTTATGGTA GAATACAAAG GAGAAAGACA AATGACATAG AGTTTACTTG CTATTAAATT 
GATTCGCTAG TAACGAAGAG CCCTATTTAA AAGATTTTCA TTTATCAGCT AGATTATTAA 
AAAACCAATT ACACATGTGT ACTTGTATGT GATGATTAGA CCTTTAATTA CTTCTATGAT 
GAGCAGCCTT TGCCAAATCT TTTTAAAAAG TATGAAAAAG GAAGTGAAAA TCTTGATGAA 
ATACCAATCA ATCCTCCAGG AAATATAATT ATACATATGG CTAAAAAAAT TGGTTGTAAT 
AGACCTCATT CTTTTGCTTA TACCACAGGT TCTACTATAA TATGTCTATA ATTATCATCT 
GTTAATGAAT TACCCAACGA CTCTTTCTTG AAAAACTTTA AATTTCTTTA GTATGCTAAA 
ACAGAAGAAG ATTCTCGTAA AGATGTGAAA GATAGATTTT TAATTGAAGA AAGAATCCTC 
AAAATAGGAT TAACAGAATT TCATTACTTT TTGCTTCATG AGGACTGTCT AACAGTAATG 
AGTAGATTAG ATTAAAAGGT AGTTACTACA TTTGAACTGG GAAATATGTA GGGACCTGTG 
ATTGATATGA TCTACGATGA TTCTTCCAGT TAAAGATCTT TTCTTATTTA TTCTAGAAAG 
AGAATTTTAA AAATTGTGAT TAACATGGAA GAAGAATTTG CATGCTATGA TTTAATTAAG 
TAAAAAGATA TAAAAGGTGC TTACGAGCTA TGCAAGAGAA CAAATTTGGA CTGTCTTCCT 
TATGTTTCTG GTTTATATGG AGATTATTTG TATTAGGAAG GAAAGTATGT GACTGCTGCA 
TAAGTTTATT CAACATCATA AAAAACATTC GAAGAAATTA TGATTAAGTT CTTAAAAAAC 
GAATCACTTG AATAGATTGA AGGCATAATA GAGTATCTGT AAATTTAAGC TGCAAGACTC 
AATAATTAGA AAAATTAGGT TTAATTTAAA ATAATTTCAG GATTGAGCTT AGAATATTAC 
AACTATAAAA TTAATGAATT AGAGAAGTAT TTAGAAGTGC TTATTTAAGA GGAGGCATAA 
AAAGAATTAG AAAAGTAATC AGACTAACAT CTTAAATTTA AAATAGAATA TAGAGGCAAT 
TAGCTACAGG TAGTTTATTT AGATAATTTA AATGAAATGA GATTGAGAAA GCAAAGTGGT 
GAAGATCAAA CACCAATGTC TTAGCCCTCA AATAAATTGA GTAACGATGG TTAAGCTGAT 
TAAAAAAGAA AAGCTTCTTA AAATTCTATC ACAAGTAAAA GAATTGTCAG GATATAAAGC 
AAGCTTAAAA AAATAAAAGA TAATTATAAC TCATTTTTGA AAAATCTTAA TTCTTAAATA 
GATCAAGAAT TCGTTTATGA AATAACTCAA AGTCATGGAA GAATTGATGA CTGCTTACAG 
TATGCAAAGG AGTAAAATAA TTATGAAAAT GTTATAACAC ATTATGTAAA CGAATAAAAT 
TACAAAGAAG CTGTCAGCTA TCTAAAAGTT TTAGCCAAAA AAGACAGCAA AAGAGCCATA 
GAAATCATAT ACAAATTTTC TTACATTTTA CTCTAAAAAG TTCCTGAAGA ATTCATTTCT 
TTGGTCTTAA ACTCTATCAG AGATTTCCAA CCTTCTAAAT TGATATCAGG ATTAATGAAT 
ATTCCAGGCA CCTTTAGAAA ATTTGGAATC TAATTTTTAG AAAATTGTGT TTATAAATAA 
AAGAGCTCTG ATAAAATCAT TCACAATCTT TATCTTTTTT TCTTGGCAAA CCAAATTCGT 
CCTGATGAAA ACAGCCAAAC TTAAAAAGTT GATGAGGAAG CTTACTAATA ACTTATGGAT 
TACTTGAACA AAGCAGAAGA ACAAGAAAAC TAGTAAATTT ATGGAAGTTT ATAGAATGAT 
GATTCTGAAA TAAGTTTGTA TTCATAAAAA TCATCAAGAA ATTCTCAAAA GCAACAAAAT 
TATATTCTGA AATTTGATTT AGATTTTGCA CTTCATTTAT TTGAATCAAA AATGATTACA 
GATGCGATGG TCAAGACCTA TGCTTTGATG AATTTTTATG ATTTAGCAGT TAAATTAGCT 
TTAGATAAAA ATAACATTAG CCTTGCTAAA GAATATGCTT CAAAGCACCA TAATGATGAT 
AAAAAACATA AATTGTGGCT CAAAATAGCA GTTCATTTAA TTTAAACATA GTAATCCCAA 
AATGTTCTAT AATTAACAAA TGACAGTAAT GGTTATTTAA AAGTAGAGGA TCTTCTTCCA 
CATTTTGGAG AAGTTCATCA AATAGACCTA CTCAAAGAAT AAATTTGCAG TAATTTAGAA 
GACTATAACA ATGAGATTGA AAATTTGAAG AAAGAAATGG ATTAACTAAA CGAAATGTCA 
GATTCACTTA AGCGTGAAAT TAATACTTAT TCTCATAAAT CTCTATAGAT TAAGGGAGAA 
TAGGATTGCA GTATATGTCA CAGAAACCTT CTTTCATAAG ATCATTATTT CTTTGGCTGC 
TTCCATGGTT TTCATAGAAA TTGCTACATT TAAAGAATGT AAAGTGATAT AATAGCTGAA 
TAAGAGAAAG AATAGATAAG CGATTAAGGC GAAAAATAAC TAACTTCTTA TTCTCAAAAG 
AGAGTTTTTG ATAAAATAAT ATCAATAAAT TAGTACATCA ATAAAATCTA ATCGGATTTA 
AAAAATAAAC AAGAAGAAGA AATTAAGTAA ATGAAATTAA TGTTAATAGA TGAAAATCAA 
TAATAGTAAT CCTCTTCAAT TACTAAATTC TTTAAAGGAC TTGGAGGTGC TTTAATAGGT 
GGAGCAGGTA AAAAAGAAGA TGAATAATAA GCTACTATAG ATATTAATGC TTTCAAAAAA 
AAACTAAGTT CTATGGCAAA TCTCAAAGAT ATGTTATAAT TATTTTTAAG TTAAGAAGAA 
AGGGATGAAT ATTATAAACA AGTATAAATT GTTGATGACT TTCTCTCAAA AGAGTGTTAT 
TTTTGTGGAC TTTCTTCTGT TGATCGTATT CATACATTTT ATCCTAAAAA TACTAAGAGT 
TCTATTGATT GGGCGATTTG A