plcb2 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	plcb2
Entrez Gene ID	101076025
Full Name	phospholipase C beta 2
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029833020.1	XP_029688880.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2
XM_011619213.1	XP_011617515.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

Pathways from BioSystems
Gene Ontology

The following plcb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plcb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo73435	XM_029833020.1 Latest version!	Takifugu rubripes phospholipase C beta 2 (plcb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo29380	XM_011619213.1 Latest version!	Takifugu rubripes phospholipase C, beta 2 (plcb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

plcb2 ( XM_029833020.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688880.1 Takifugu rubripes phospholipase C beta 2 (plcb2), mRNA.

Price & Availability↑

CloneID	OTo73435
Clone ID Related Accession (Same CDS sequence)	XM_029833020.1
Accession Version	XM_029833020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042286.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGA AGAGGCACTT TTTAGAGCCT CCGGAGGTCA AGGACTACAT GGTCAAAGGA 
GAGCGATTCA CCAAATGGTC AGAGGACTCC ACAAAGACTG CTCCTGTCAC CATGAAGATG 
GACCCGAAAG GATTCTACCT CTACTGGATC AACCAAAGCA AGGAGACAAC GTTCCTGGAT 
GTTGCTACTA TCAGAGATAC CAGAACAGGA AAATATGCCA AACTTCCAAA ACACCCTAAA 
GTTCGCAACG TGTTTAACCT GGACTTCCCT GACAGCAACC ATCTCGCCAA GACTCTGACT 
GTTGTCTCAG GACCAGACAC GGTCAATTTG ACCTATCACA ACTTCTTTGC CTCTAAGGAG 
AAGGTGACAC AGAACTGGGC CAATGACATC ATTGCACTTG TGTATAACGC TGCCAGGAAC 
AACGCATGCA AACAAGTCTT CCTGGAGAAA ATATACGTTC GCGTTTCCCT CCACACCAAC 
AAGGACGGCA AGATCCCGGT GAAAAACATC TACAAGATGT TCCCGGCAGA TAAGAAAAGA 
GTTGAAAGTG CGTTGGCATC GGCCCACCTC CCCAAAGGAA AGTACGACAC CATGAAGCCT 
GATGTCTTCA CTGAGGCTGC CTTCAAGGTC TTTCTGGCCA ACCTCTGCCC TCGTCCTGAG 
ATCCTAGAGA TCTTCACCTC TTATTCCAAC AAACCGACCA TGACGAAGGA AAACTTCACT 
AAATTCCTCA ACGAGAAGCA GAGGGACTCC CGGCTCAACG AGGAGCTCTT CCCGCGCCTG 
CGGCAGGACC AGGTCAAGGC CCTGATCGAC AAATACGAGC CCTGCTCCTC CAACTCGAAC 
AGAAGTCTCA TTTCTCCTGA AGGCCTCTTA AATTTCCTGA CTGGTCCAGA GACATCCGTG 
GTGATTCTGG ACCGGCTGGC CAAGTGGCAG GACATGACCC AACCGTTACC CCACTACTTC 
GTCAAGTCCT CCCACAACAC ATACCTGACA GCTGGTCAGT TCTCCGGCGT GTCCTCGCCC 
GAGATGTACC GACAGTGTCT GTTGGCCGGC TGCCGCTGCC TGGAGCTGGA CTGCTGGAAG 
GGCAAACCCC CAGATGAAGA GCCCATCATT ACCCACGGCT TCACCATGAC GACTGAGATC 
CTCTTCAAGG ACGTAATCGA GGCTATCGCT GAGAGCGCTT TCAAGACCTC CCAGTATCCT 
GTCATCCTCT CGTTCGAGAA CCATGTTGAC ACAGTGAAAC AGCAGGAGAA AATGGCAAAC 
TACTGCAAAA CCATATTTGG TGAGGCCCTG CTAACAGAGC CGCTGGACAA ATACCCCCTG 
AAGCCGGGCT GCCAGATCCC CAGTCCATCT GAGCTGATGG GCAAGATCCT CATCAAGAAC 
AAGAAGAGCA GCAATGAAAC CCAGACACAT ACTAAAAAAA CCCAGGATGC TGCCAGCACA 
GCTACCAGCA CGCAGGAGAA CCAGGCTAGT TCAGAGGGGG GCGCTGCTGC GGAGGACACC 
GAGGAGCAGG AGGACACGGA GGAGCAGGAT GAGGAGAAAA TGAAGACGTC AGATGAGGGC 
ACAGCTGGAC AGGAAGTGAC AGCTTTCGAA GCGATGTCGT CCCTGGTCAA CTACATCCAG 
CCCAACAAAT TCATCTCTTT TGACAACGCC AGAAAGAAAA ACAAGAGTTA TGTCATCTCA 
TCTTTCGTGG AGACAAAAGG GGAGTCAATG ATTGCCAAGA ATGCTGTTGA ATTTGTTGAA 
TATAATAAAA GGCAGATGAG CAGGATTTAC CCTAAAGGAA CAAGAGTGGA TTCGTCTAAT 
TACAGCCCAC AGCCTTTCTG GAACGTCGGC TGCCAGATGG TGGCGCTCAA CTACCAGACC 
ATGGATTTCC CCATGCAGCT GAACATGGCT CTGTTTGAGT TCAACGGCAG AACCGGTTAC 
CTGCTCAAGC ATGACGTGTT GCGTCGCAGT GACAAGACGT TTAACCCTTT CTGCGACAGG 
ATAGACACAG TTGTGGCAAG CACGCTCACC ATAAAGATTT ATTCAGGCCA GTTTTTGTCT 
GACAAAAATG TGAAAACCGG CATCGAGGTG GAGGTGATTG GGCTGCCGGG GGACCCCAAG 
AAGAAATTTC GCACCAAGTG GTCCCCCACA CCCAACAGTA TTAACCCGGT GTGGAACGAG 
GAGCCTTTTG TGTTCGAAAA GATCCTGCTT CCAGAAATGG CCTCTCTGAG AATTGTGGCT 
CACGAAGAGA ACGGTAAATT CTTGGGACAC AGGATCATCC CCCTTGATGC CATCCAATCA 
GGTTTCCATC ACATCTGCCT GCACAGTGAG AGCAACATGC CGCTCACTCT GCCCTCTGTC 
TTCGTGTACA TCGAGGTCAA GGACTACATC CCTGCTGCAT TCGCAGATTT TACAGATGCC 
TTATTCAATC CAACAAAGGG CAACGAGAAA ACCTCAAAGA CCCCGAAGGA GTCCTCCTCA 
GACTACATTA GTCCTTATGA GCTGCCTCTC ATGACTCAGA CCTCTGCAAA CGATGCAAAG 
GAAGCTGAAT CTCCGGCTGC AGAAAAGGCT GCAGCACCAA CACCACCAGC CGATGTAGGA 
GAGGAGTCGA CTGAACCTGG ACCAGAAACA AGCACGGCCG ATCCTGCAGA GAACGTGCCA 
GAAGCTGAAG CAACCTGCTC CGAACCTGCT GCAGACGCAG CAGCTGAGGA GGCGTCTCCA 
GATCCAGGCC CTGTCACAGC TGAACCCCCC CCTGAAGCTG GTCCGCCCGC AGACGCGGCT 
GCAGCGCAGG AAGAGGTTCC AGAGCCGGAG GTGTCCAACG AGGACACAAC CAGCGACCCC 
GACACAAACA CAGAGCCGTC TCCAGGTGAC AACGCTGCAA CCGGCGCTGC TAACGAAGCT 
GCTAACGAAG CTGCTAATGA AGCTGCTAAC GAAGCTGCTG CTGTAGCTGG TGCGGCTGGC 
GGAGCGGCCC AGTCCGCCGG AGGTGAAGGC TCAGCCCCGG CGCAGATGTG CGGTGAAGAG 
CACGCCACGG TGACAGCTGA GGAACTGACA CAGCACAAGA ACTACCTGAA GGCCATCAAA 
CGCCAGGAGA GAGACATGAA GGCGGCAGAG AAGAAATATC AGAAAAAGAG CGAGGATCTG 
ATCCAGAAAT ACTCCGACAC CTTCAAGGCC ATCAAGAAGA AGATCTCATT GAAGAAAACA 
GAGGGAGGGG AAAACTCCAC GGACAGCGGC GTGAAGAGCG AGCGTGTGCA GGACCATAAA 
GAAAAAATGC TGGCTGAGCT GCAGCTGCAG TGGAAGGAAC AGTGTGACCA TCTGAGGAAG 
AAGAAGGAGC AGTTTGCAAC AGAGAGGCTG GCCGTGCTGC TGGAGATGGC AACAGAGCGG 
CACAACAGTG AACTGAAGAT GCTGGAGAGT GAACCCAAAG ATAACAAGAA GAAAACCAAA 
TGCTCGGACA AAGCCAAGTT GAAAAGGGCG ATGAGCACAG AGCTGTTGGA TGGGCCTTGT 
TTGATGGCTT GTTCGTCTGC AGGTGGCAGC CCGCAGCAAG AGGCTCTGAT GAAGAAACAG 
TGCGCTACAC TGGATGAAAT CATGACTTTG ACCAATCAGC TCAATCAGGA GGCCCTGAGG 
GAGCATGAGC AGAAATTGAG GGGCCTGTCT GCAGAGGTGA GAGGGGCCGT CAACGTCTGC 
CTGGGGGCCC ACTTCCCTGA CCTGGTGGAG CAAGCTGAGG ACAAGAAGTT GGAGGGGATG 
GACTTCTATG GGGACGTGTT CCTGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688880.1
CDS	1..3750
Translation	MNQKRHFLEPPEVKDYMVKGERFTKWSEDSTKTAPVTMKMDPKGFYLYWINQSKETTFLDVATIRDTRTGKYAKLPKHPKVRNVFNLDFPDSNHLAKTLTVVSGPDTVNLTYHNFFASKEKVTQNWANDIIALVYNAARNNACKQVFLEKIYVRVSLHTNKDGKIPVKNIYKMFPADKKRVESALASAHLPKGKYDTMKPDVFTEAAFKVFLANLCPRPEILEIFTSYSNKPTMTKENFTKFLNEKQRDSRLNEELFPRLRQDQVKALIDKYEPCSSNSNRSLISPEGLLNFLTGPETSVVILDRLAKWQDMTQPLPHYFVKSSHNTYLTAGQFSGVSSPEMYRQCLLAGCRCLELDCWKGKPPDEEPIITHGFTMTTEILFKDVIEAIAESAFKTSQYPVILSFENHVDTVKQQEKMANYCKTIFGEALLTEPLDKYPLKPGCQIPSPSELMGKILIKNKKSSNETQTHTKKTQDAASTATSTQENQASSEGGAAAEDTEEQEDTEEQDEEKMKTSDEGTAGQEVTAFEAMSSLVNYIQPNKFISFDNARKKNKSYVISSFVETKGESMIAKNAVEFVEYNKRQMSRIYPKGTRVDSSNYSPQPFWNVGCQMVALNYQTMDFPMQLNMALFEFNGRTGYLLKHDVLRRSDKTFNPFCDRIDTVVASTLTIKIYSGQFLSDKNVKTGIEVEVIGLPGDPKKKFRTKWSPTPNSINPVWNEEPFVFEKILLPEMASLRIVAHEENGKFLGHRIIPLDAIQSGFHHICLHSESNMPLTLPSVFVYIEVKDYIPAAFADFTDALFNPTKGNEKTSKTPKESSSDYISPYELPLMTQTSANDAKEAESPAAEKAAAPTPPADVGEESTEPGPETSTADPAENVPEAEATCSEPAADAAAEEASPDPGPVTAEPPPEAGPPADAAAAQEEVPEPEVSNEDTTSDPDTNTEPSPGDNAATGAANEAANEAANEAANEAAAVAGAAGGAAQSAGGEGSAPAQMCGEEHATVTAEELTQHKNYLKAIKRQERDMKAAEKKYQKKSEDLIQKYSDTFKAIKKKISLKKTEGGENSTDSGVKSERVQDHKEKMLAELQLQWKEQCDHLRKKKEQFATERLAVLLEMATERHNSELKMLESEPKDNKKKTKCSDKAKLKRAMSTELLDGPCLMACSSAGGSPQQEALMKKQCATLDEIMTLTNQLNQEALREHEQKLRGLSAEVRGAVNVCLGAHFPDLVEQAEDKKLEGMDFYGDVFLG

Target ORF information:

RefSeq Version	XM_029833020.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phospholipase C beta 2 (plcb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029833020.1

ORF Insert Sequence:

ATGAACCAGA AGAGGCACTT TTTAGAGCCT CCGGAGGTCA AGGACTACAT GGTCAAAGGA 
GAGCGATTCA CCAAATGGTC AGAGGACTCC ACAAAGACTG CTCCTGTCAC CATGAAGATG 
GACCCGAAAG GATTCTACCT CTACTGGATC AACCAAAGCA AGGAGACAAC GTTCCTGGAT 
GTTGCTACTA TCAGAGATAC CAGAACAGGA AAATATGCCA AACTTCCAAA ACACCCTAAA 
GTTCGCAACG TGTTTAACCT GGACTTCCCT GACAGCAACC ATCTCGCCAA GACTCTGACT 
GTTGTCTCAG GACCAGACAC GGTCAATTTG ACCTATCACA ACTTCTTTGC CTCTAAGGAG 
AAGGTGACAC AGAACTGGGC CAATGACATC ATTGCACTTG TGTATAACGC TGCCAGGAAC 
AACGCATGCA AACAAGTCTT CCTGGAGAAA ATATACGTTC GCGTTTCCCT CCACACCAAC 
AAGGACGGCA AGATCCCGGT GAAAAACATC TACAAGATGT TCCCGGCAGA TAAGAAAAGA 
GTTGAAAGTG CGTTGGCATC GGCCCACCTC CCCAAAGGAA AGTACGACAC CATGAAGCCT 
GATGTCTTCA CTGAGGCTGC CTTCAAGGTC TTTCTGGCCA ACCTCTGCCC TCGTCCTGAG 
ATCCTAGAGA TCTTCACCTC TTATTCCAAC AAACCGACCA TGACGAAGGA AAACTTCACT 
AAATTCCTCA ACGAGAAGCA GAGGGACTCC CGGCTCAACG AGGAGCTCTT CCCGCGCCTG 
CGGCAGGACC AGGTCAAGGC CCTGATCGAC AAATACGAGC CCTGCTCCTC CAACTCGAAC 
AGAAGTCTCA TTTCTCCTGA AGGCCTCTTA AATTTCCTGA CTGGTCCAGA GACATCCGTG 
GTGATTCTGG ACCGGCTGGC CAAGTGGCAG GACATGACCC AACCGTTACC CCACTACTTC 
GTCAAGTCCT CCCACAACAC ATACCTGACA GCTGGTCAGT TCTCCGGCGT GTCCTCGCCC 
GAGATGTACC GACAGTGTCT GTTGGCCGGC TGCCGCTGCC TGGAGCTGGA CTGCTGGAAG 
GGCAAACCCC CAGATGAAGA GCCCATCATT ACCCACGGCT TCACCATGAC GACTGAGATC 
CTCTTCAAGG ACGTAATCGA GGCTATCGCT GAGAGCGCTT TCAAGACCTC CCAGTATCCT 
GTCATCCTCT CGTTCGAGAA CCATGTTGAC ACAGTGAAAC AGCAGGAGAA AATGGCAAAC 
TACTGCAAAA CCATATTTGG TGAGGCCCTG CTAACAGAGC CGCTGGACAA ATACCCCCTG 
AAGCCGGGCT GCCAGATCCC CAGTCCATCT GAGCTGATGG GCAAGATCCT CATCAAGAAC 
AAGAAGAGCA GCAATGAAAC CCAGACACAT ACTAAAAAAA CCCAGGATGC TGCCAGCACA 
GCTACCAGCA CGCAGGAGAA CCAGGCTAGT TCAGAGGGGG GCGCTGCTGC GGAGGACACC 
GAGGAGCAGG AGGACACGGA GGAGCAGGAT GAGGAGAAAA TGAAGACGTC AGATGAGGGC 
ACAGCTGGAC AGGAAGTGAC AGCTTTCGAA GCGATGTCGT CCCTGGTCAA CTACATCCAG 
CCCAACAAAT TCATCTCTTT TGACAACGCC AGAAAGAAAA ACAAGAGTTA TGTCATCTCA 
TCTTTCGTGG AGACAAAAGG GGAGTCAATG ATTGCCAAGA ATGCTGTTGA ATTTGTTGAA 
TATAATAAAA GGCAGATGAG CAGGATTTAC CCTAAAGGAA CAAGAGTGGA TTCGTCTAAT 
TACAGCCCAC AGCCTTTCTG GAACGTCGGC TGCCAGATGG TGGCGCTCAA CTACCAGACC 
ATGGATTTCC CCATGCAGCT GAACATGGCT CTGTTTGAGT TCAACGGCAG AACCGGTTAC 
CTGCTCAAGC ATGACGTGTT GCGTCGCAGT GACAAGACGT TTAACCCTTT CTGCGACAGG 
ATAGACACAG TTGTGGCAAG CACGCTCACC ATAAAGATTT ATTCAGGCCA GTTTTTGTCT 
GACAAAAATG TGAAAACCGG CATCGAGGTG GAGGTGATTG GGCTGCCGGG GGACCCCAAG 
AAGAAATTTC GCACCAAGTG GTCCCCCACA CCCAACAGTA TTAACCCGGT GTGGAACGAG 
GAGCCTTTTG TGTTCGAAAA GATCCTGCTT CCAGAAATGG CCTCTCTGAG AATTGTGGCT 
CACGAAGAGA ACGGTAAATT CTTGGGACAC AGGATCATCC CCCTTGATGC CATCCAATCA 
GGTTTCCATC ACATCTGCCT GCACAGTGAG AGCAACATGC CGCTCACTCT GCCCTCTGTC 
TTCGTGTACA TCGAGGTCAA GGACTACATC CCTGCTGCAT TCGCAGATTT TACAGATGCC 
TTATTCAATC CAACAAAGGG CAACGAGAAA ACCTCAAAGA CCCCGAAGGA GTCCTCCTCA 
GACTACATTA GTCCTTATGA GCTGCCTCTC ATGACTCAGA CCTCTGCAAA CGATGCAAAG 
GAAGCTGAAT CTCCGGCTGC AGAAAAGGCT GCAGCACCAA CACCACCAGC CGATGTAGGA 
GAGGAGTCGA CTGAACCTGG ACCAGAAACA AGCACGGCCG ATCCTGCAGA GAACGTGCCA 
GAAGCTGAAG CAACCTGCTC CGAACCTGCT GCAGACGCAG CAGCTGAGGA GGCGTCTCCA 
GATCCAGGCC CTGTCACAGC TGAACCCCCC CCTGAAGCTG GTCCGCCCGC AGACGCGGCT 
GCAGCGCAGG AAGAGGTTCC AGAGCCGGAG GTGTCCAACG AGGACACAAC CAGCGACCCC 
GACACAAACA CAGAGCCGTC TCCAGGTGAC AACGCTGCAA CCGGCGCTGC TAACGAAGCT 
GCTAACGAAG CTGCTAATGA AGCTGCTAAC GAAGCTGCTG CTGTAGCTGG TGCGGCTGGC 
GGAGCGGCCC AGTCCGCCGG AGGTGAAGGC TCAGCCCCGG CGCAGATGTG CGGTGAAGAG 
CACGCCACGG TGACAGCTGA GGAACTGACA CAGCACAAGA ACTACCTGAA GGCCATCAAA 
CGCCAGGAGA GAGACATGAA GGCGGCAGAG AAGAAATATC AGAAAAAGAG CGAGGATCTG 
ATCCAGAAAT ACTCCGACAC CTTCAAGGCC ATCAAGAAGA AGATCTCATT GAAGAAAACA 
GAGGGAGGGG AAAACTCCAC GGACAGCGGC GTGAAGAGCG AGCGTGTGCA GGACCATAAA 
GAAAAAATGC TGGCTGAGCT GCAGCTGCAG TGGAAGGAAC AGTGTGACCA TCTGAGGAAG 
AAGAAGGAGC AGTTTGCAAC AGAGAGGCTG GCCGTGCTGC TGGAGATGGC AACAGAGCGG 
CACAACAGTG AACTGAAGAT GCTGGAGAGT GAACCCAAAG ATAACAAGAA GAAAACCAAA 
TGCTCGGACA AAGCCAAGTT GAAAAGGGCG ATGAGCACAG AGCTGTTGGA TGGGCCTTGT 
TTGATGGCTT GTTCGTCTGC AGGTGGCAGC CCGCAGCAAG AGGCTCTGAT GAAGAAACAG 
TGCGCTACAC TGGATGAAAT CATGACTTTG ACCAATCAGC TCAATCAGGA GGCCCTGAGG 
GAGCATGAGC AGAAATTGAG GGGCCTGTCT GCAGAGGTGA GAGGGGCCGT CAACGTCTGC 
CTGGGGGCCC ACTTCCCTGA CCTGGTGGAG CAAGCTGAGG ACAAGAAGTT GGAGGGGATG 
GACTTCTATG GGGACGTGTT CCTGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb2 ( XM_011619213.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011617515.1 Takifugu rubripes phospholipase C, beta 2 (plcb2), mRNA.

Price & Availability↑

CloneID	OTo29380
Clone ID Related Accession (Same CDS sequence)	XM_011619213.1
Accession Version	XM_011619213.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070921.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 6% of CDS bases ##RefSeq-Attributes-END##

ATGAACCAGA AGAGGCACTT TTTAGAGCCT CCGGAGGTCA AGGACTACAT GGTCAAAGGA 
GAGCGATTCA CCAAATGGTC AGAGGACTCC ACAAAGACTG CTCCTGTCAC CATGAAGATG 
GACCCGAAAG GATTCTACCT CTACTGGATC AACCAAAGCA AGGAGACAAC GTTCCTGGAT 
GTTGCTACTA TCAGAGATAC CAGAACAGGA AAATATGCCA AACTTCCAAA ACACCCTAAA 
GTTCGCAACG TGTTTAACCT GGACTTCCCT GACAGCAACC ATCTCGCCAA GACTCTGACT 
GTCGTCTCGG GACCAGACAC GGTCAATTTG ACCTATCACA ACTTCTTTGC CTCTAAGGAG 
AAGGTGACAC AGAACTGGGC CAATGACATC ATTGCACTTG CGTATAACGC TGCCAGGAAC 
AACGCATGCA AACAAGTCTT CCTGGAGAAA ATATACGTTC GCGTTTCCCT CCACACCAAC 
AAGGACGGCA AGATCCCGGT GAAAAACATC TACAAGATGT TCCCGGCAGA TAAGAAAAGA 
GTTGAAAGTG CGTTGGCATC GGCCCACCTC CCCAAAGGAA AGTACGACAC CATGAAGCCT 
GATGTCTTCA CTGAGGCTGC CTTCAAGGTC TTTCTGGCCA ACCTCTGCCC TCGTCCTGAG 
ATCCTAGAGA TCTTCACCTC TTATTCCAAC AAACCGACCA TGACGAAGGA AAACTTCACT 
AAATTCCTCA ACGAGAAGCA GAGGGACTCC CGGCTCAACG AGGAGCTCTT CCCGCGCCTG 
CGGCAGGACC AGGTCAAGGC CCTGATCGAC AAATACGAGC CCTGCTCCTC CAACTCGAAC 
AGAAGTCTCA TTTCTCCTGA AGGCCTCTTA AATTTCCTGA CTGGTCCAGA GACATCCGTG 
GTGATTCTGG ACCGGCTGGC CAAGTGGCAG GACATGACCC AACCGTTACC CCACTACTTC 
GTCAAGTCCT CCCACAACAC ATACCTGACA GCTGGTCAGT TCTCCGGCGT GTCCTCGCCC 
GAGATGTACC GACAGTGTCT GTTGGCCGGC TGCCGCTGCC TGGAGCTGGA CTGCTGGAAG 
GGCAAACCCC CAGATGAAGA GCCCATCATT ACCCACGGCT TCACCATGAC GACTGAGATC 
CTCTTCAAGG ACGTAATCGA GGCTATCGCT GAGAGCGCTT TCAAGACCTC CCAGTATCCT 
GTCATCCTCT CGTTCGAGAA CCATGTTGAC ACAGTGAAAC AGCAGGAGAA AATGGCAAAC 
TACTGCAAAA CCATATTTGG TGAGGCCCTG CTAACAGAGC CGCTGGACAA ATACCCCCTG 
AAGCCGGGCT GCCAGATCCC CAGTCCATCT GAGCTGATGG GCAAGATCCT CATCAAGAAC 
AAGAAGAGCA GCAATGAAAC CCAGACACAT ACTAAAAAAA CCCAGGATGC TGCCAGCACA 
GCTACCAGCA CGCAGGAGAA CCAGGCTAGT TCAGAGGGGG GCGCTGCTGC GGAGGACACC 
GAGGAGCAGG AGGACACGGA GGAGCAGGAT GAGGAGAAAA TGAAGACGTC AGATGAGGGC 
ACAGCTGGAC AGGAAGTGAC AGCTTTCGAA GCGATGTCGT CCCTGGTCAA CTACATCCAG 
CCCAACAAAT TCATCTCTTT TGACAACGCC AGAAAGAAAA ACAAGAGTTA TGTCATCTCA 
TCTTTCGTGG AGACAAAAGG GGAGTCAATG ATTGCCAAGA ATGCTGTTGA ATTTGTTGAA 
TATAATAAAA GGCAGATGAG CAGGATTTAC CCTAAAGGAA CAAGAGTGGA TTCGTCTAAT 
TACAGCCCAC AGCCTTTCTG GAACGTCGGC TGCCAGATGG TGGCGCTCAA CTACCAGACC 
ATGGATTTCC CCATGCAGCT GAACATGGCT CTGTTTGAGT TCAACGGCAG AACCGGTTAC 
CTGCTCAAGC ATGACGTGTT GCGTCGCAGT GACAAGACGT TTAACCCTTT CTGCGACAGG 
ATAGACACAG TTGTGGCAAG CACGCTCACC ATAAAGATTT ATTCAGGCCA GTTTTTGTCT 
GACAAAAATG TGAAAACCGG CATCGAGGTG GAGGTGATTG GGCTGCCGGG GGACCCCAAG 
AAGAAATTTC GCACCAAGTG GTCCCCCACA CCCAACAGTA TTAACCCGGT GTGGAACGAG 
GAGCCTTTTG TGTTCGAAAA GATCCTGCTT CCAGAAATGG CCTCTCTGAG AATTGTGGCT 
CACGAAGAGA ACGGTAAATT CTTGGGACAC AGGATCATCC CCCTTGATGC CATCCAATCA 
GGTTTCCATC ACATCTGCCT GCACAGTGAG AGCAACATGC CGCTCACTCT GCCCTCTGTC 
TTCGTGTACA TCGAGGTCAA GGACTACATC CCTGCTGCAT TCGCAGATTT TACAGATGCC 
TTATTCAATC CAACAAAGGG CAACGAGAAA ACCTCAAAGA CCCCGAAGGA GTCCTCCTCA 
GACTACATTA GTCCTTATGA GCTGCCTCTC ATGACTCAGA CCTCTGCAAA CGATGCAAAG 
GAAGCTGAAT CTCCGGCTGC AGAAAAGGCT GCAGCACCAA CACCACCAGC CGATGTAGGA 
GAGGAGTCGA CTGAACCTCG ACCAGAAACA AGCACGGCCG ATCCTGCAGA GAACGTGCCA 
GAAGCTGAAG CAACCTGCTC CGAACCTGCT GCAGACGCAG CAGCTGAGGA GGCGTCTCCA 
GATCCAGGCC CTGTCACAGC TGAACCCCCC CCTGAAGCTG GTCCGCCCGC AGACGCGGCT 
GCAGAGCAGG AAGAGGTTCC AGAGCCGGAG GTGTCCAACG AGGACACAAC CAGCGACCCC 
GACACAAACA CAGAGCCGTC TCCAGGTGAC AACGCTGCAG CCGGCGCTGC TAACGAAGCT 
GCTAACGAAG CTGCTAATGA AGCTGCTAAC GAAGCTGCTG CTGTAGCTGG TGCGGCTGGC 
GGAGCGGCCC AGTCCGCCGG AGGTGAAGGC TCAGCCCCGG CGCAGATGTG CGGTGAAGAG 
CACGCCACGG TGACAGCTGA GGAACTGACA CAGCACAAGA ACTACCTGAA GGCCATCAAA 
CGCCAGGAGA GAGACATGAA GGCGGCAGAG AAGAAATATC AGAAAAAGAG CGAGGATCTG 
ATCCAGAAAT ACTCCGACAC CTTCAAGGCC ATCAAGAAGA AGATCTCATT GAAGAAAACA 
GAGGGAGGGG AAAACTCCAC GGACAGCGGC GTGAAGAGCG AGCGTGTGCA GGACCATAAA 
GAAAAAATGC TGGCTGAGCT GCAGCTGCAG TGGAAGGAAC AGTGTGACCA TCTGAGGAAG 
AAGAAGGAGC AGTTTGCAAC AGAGAGGCTG GCCGTGCTGC TGGAGATGGC AACAGAGCGG 
CACAACAGTG AACTGAAGAT GCTGGAGAGT GAACCCAAAG ATAACAAGAA GAAAACCAAA 
TGCTCGGACA AAGCCAAGTT GAAAAGGGCG ATGAGCACAG AGCTGTTGGA TGGGCCTTGT 
TTGATGGCTT GTTCGCTGGA GGAGGAGGGT CTGAGCGGGG CCCTGGTCAC CATGGAGGGG 
TCGACAGGAA GAAGCACACA AGGACAAATG ATTGACATGT ACAAAGACTG TGACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011617515.1
CDS	1..3600
Translation	MNQKRHFLEPPEVKDYMVKGERFTKWSEDSTKTAPVTMKMDPKGFYLYWINQSKETTFLDVATIRDTRTGKYAKLPKHPKVRNVFNLDFPDSNHLAKTLTVVSGPDTVNLTYHNFFASKEKVTQNWANDIIALAYNAARNNACKQVFLEKIYVRVSLHTNKDGKIPVKNIYKMFPADKKRVESALASAHLPKGKYDTMKPDVFTEAAFKVFLANLCPRPEILEIFTSYSNKPTMTKENFTKFLNEKQRDSRLNEELFPRLRQDQVKALIDKYEPCSSNSNRSLISPEGLLNFLTGPETSVVILDRLAKWQDMTQPLPHYFVKSSHNTYLTAGQFSGVSSPEMYRQCLLAGCRCLELDCWKGKPPDEEPIITHGFTMTTEILFKDVIEAIAESAFKTSQYPVILSFENHVDTVKQQEKMANYCKTIFGEALLTEPLDKYPLKPGCQIPSPSELMGKILIKNKKSSNETQTHTKKTQDAASTATSTQENQASSEGGAAAEDTEEQEDTEEQDEEKMKTSDEGTAGQEVTAFEAMSSLVNYIQPNKFISFDNARKKNKSYVISSFVETKGESMIAKNAVEFVEYNKRQMSRIYPKGTRVDSSNYSPQPFWNVGCQMVALNYQTMDFPMQLNMALFEFNGRTGYLLKHDVLRRSDKTFNPFCDRIDTVVASTLTIKIYSGQFLSDKNVKTGIEVEVIGLPGDPKKKFRTKWSPTPNSINPVWNEEPFVFEKILLPEMASLRIVAHEENGKFLGHRIIPLDAIQSGFHHICLHSESNMPLTLPSVFVYIEVKDYIPAAFADFTDALFNPTKGNEKTSKTPKESSSDYISPYELPLMTQTSANDAKEAESPAAEKAAAPTPPADVGEESTEPRPETSTADPAENVPEAEATCSEPAADAAAEEASPDPGPVTAEPPPEAGPPADAAAEQEEVPEPEVSNEDTTSDPDTNTEPSPGDNAAAGAANEAANEAANEAANEAAAVAGAAGGAAQSAGGEGSAPAQMCGEEHATVTAEELTQHKNYLKAIKRQERDMKAAEKKYQKKSEDLIQKYSDTFKAIKKKISLKKTEGGENSTDSGVKSERVQDHKEKMLAELQLQWKEQCDHLRKKKEQFATERLAVLLEMATERHNSELKMLESEPKDNKKKTKCSDKAKLKRAMSTELLDGPCLMACSLEEEGLSGALVTMEGSTGRSTQGQMIDMYKDCDQ

Target ORF information:

RefSeq Version	XM_011619213.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phospholipase C, beta 2 (plcb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011619213.1

ORF Insert Sequence:

ATGAACCAGA AGAGGCACTT TTTAGAGCCT CCGGAGGTCA AGGACTACAT GGTCAAAGGA 
GAGCGATTCA CCAAATGGTC AGAGGACTCC ACAAAGACTG CTCCTGTCAC CATGAAGATG 
GACCCGAAAG GATTCTACCT CTACTGGATC AACCAAAGCA AGGAGACAAC GTTCCTGGAT 
GTTGCTACTA TCAGAGATAC CAGAACAGGA AAATATGCCA AACTTCCAAA ACACCCTAAA 
GTTCGCAACG TGTTTAACCT GGACTTCCCT GACAGCAACC ATCTCGCCAA GACTCTGACT 
GTCGTCTCGG GACCAGACAC GGTCAATTTG ACCTATCACA ACTTCTTTGC CTCTAAGGAG 
AAGGTGACAC AGAACTGGGC CAATGACATC ATTGCACTTG CGTATAACGC TGCCAGGAAC 
AACGCATGCA AACAAGTCTT CCTGGAGAAA ATATACGTTC GCGTTTCCCT CCACACCAAC 
AAGGACGGCA AGATCCCGGT GAAAAACATC TACAAGATGT TCCCGGCAGA TAAGAAAAGA 
GTTGAAAGTG CGTTGGCATC GGCCCACCTC CCCAAAGGAA AGTACGACAC CATGAAGCCT 
GATGTCTTCA CTGAGGCTGC CTTCAAGGTC TTTCTGGCCA ACCTCTGCCC TCGTCCTGAG 
ATCCTAGAGA TCTTCACCTC TTATTCCAAC AAACCGACCA TGACGAAGGA AAACTTCACT 
AAATTCCTCA ACGAGAAGCA GAGGGACTCC CGGCTCAACG AGGAGCTCTT CCCGCGCCTG 
CGGCAGGACC AGGTCAAGGC CCTGATCGAC AAATACGAGC CCTGCTCCTC CAACTCGAAC 
AGAAGTCTCA TTTCTCCTGA AGGCCTCTTA AATTTCCTGA CTGGTCCAGA GACATCCGTG 
GTGATTCTGG ACCGGCTGGC CAAGTGGCAG GACATGACCC AACCGTTACC CCACTACTTC 
GTCAAGTCCT CCCACAACAC ATACCTGACA GCTGGTCAGT TCTCCGGCGT GTCCTCGCCC 
GAGATGTACC GACAGTGTCT GTTGGCCGGC TGCCGCTGCC TGGAGCTGGA CTGCTGGAAG 
GGCAAACCCC CAGATGAAGA GCCCATCATT ACCCACGGCT TCACCATGAC GACTGAGATC 
CTCTTCAAGG ACGTAATCGA GGCTATCGCT GAGAGCGCTT TCAAGACCTC CCAGTATCCT 
GTCATCCTCT CGTTCGAGAA CCATGTTGAC ACAGTGAAAC AGCAGGAGAA AATGGCAAAC 
TACTGCAAAA CCATATTTGG TGAGGCCCTG CTAACAGAGC CGCTGGACAA ATACCCCCTG 
AAGCCGGGCT GCCAGATCCC CAGTCCATCT GAGCTGATGG GCAAGATCCT CATCAAGAAC 
AAGAAGAGCA GCAATGAAAC CCAGACACAT ACTAAAAAAA CCCAGGATGC TGCCAGCACA 
GCTACCAGCA CGCAGGAGAA CCAGGCTAGT TCAGAGGGGG GCGCTGCTGC GGAGGACACC 
GAGGAGCAGG AGGACACGGA GGAGCAGGAT GAGGAGAAAA TGAAGACGTC AGATGAGGGC 
ACAGCTGGAC AGGAAGTGAC AGCTTTCGAA GCGATGTCGT CCCTGGTCAA CTACATCCAG 
CCCAACAAAT TCATCTCTTT TGACAACGCC AGAAAGAAAA ACAAGAGTTA TGTCATCTCA 
TCTTTCGTGG AGACAAAAGG GGAGTCAATG ATTGCCAAGA ATGCTGTTGA ATTTGTTGAA 
TATAATAAAA GGCAGATGAG CAGGATTTAC CCTAAAGGAA CAAGAGTGGA TTCGTCTAAT 
TACAGCCCAC AGCCTTTCTG GAACGTCGGC TGCCAGATGG TGGCGCTCAA CTACCAGACC 
ATGGATTTCC CCATGCAGCT GAACATGGCT CTGTTTGAGT TCAACGGCAG AACCGGTTAC 
CTGCTCAAGC ATGACGTGTT GCGTCGCAGT GACAAGACGT TTAACCCTTT CTGCGACAGG 
ATAGACACAG TTGTGGCAAG CACGCTCACC ATAAAGATTT ATTCAGGCCA GTTTTTGTCT 
GACAAAAATG TGAAAACCGG CATCGAGGTG GAGGTGATTG GGCTGCCGGG GGACCCCAAG 
AAGAAATTTC GCACCAAGTG GTCCCCCACA CCCAACAGTA TTAACCCGGT GTGGAACGAG 
GAGCCTTTTG TGTTCGAAAA GATCCTGCTT CCAGAAATGG CCTCTCTGAG AATTGTGGCT 
CACGAAGAGA ACGGTAAATT CTTGGGACAC AGGATCATCC CCCTTGATGC CATCCAATCA 
GGTTTCCATC ACATCTGCCT GCACAGTGAG AGCAACATGC CGCTCACTCT GCCCTCTGTC 
TTCGTGTACA TCGAGGTCAA GGACTACATC CCTGCTGCAT TCGCAGATTT TACAGATGCC 
TTATTCAATC CAACAAAGGG CAACGAGAAA ACCTCAAAGA CCCCGAAGGA GTCCTCCTCA 
GACTACATTA GTCCTTATGA GCTGCCTCTC ATGACTCAGA CCTCTGCAAA CGATGCAAAG 
GAAGCTGAAT CTCCGGCTGC AGAAAAGGCT GCAGCACCAA CACCACCAGC CGATGTAGGA 
GAGGAGTCGA CTGAACCTCG ACCAGAAACA AGCACGGCCG ATCCTGCAGA GAACGTGCCA 
GAAGCTGAAG CAACCTGCTC CGAACCTGCT GCAGACGCAG CAGCTGAGGA GGCGTCTCCA 
GATCCAGGCC CTGTCACAGC TGAACCCCCC CCTGAAGCTG GTCCGCCCGC AGACGCGGCT 
GCAGAGCAGG AAGAGGTTCC AGAGCCGGAG GTGTCCAACG AGGACACAAC CAGCGACCCC 
GACACAAACA CAGAGCCGTC TCCAGGTGAC AACGCTGCAG CCGGCGCTGC TAACGAAGCT 
GCTAACGAAG CTGCTAATGA AGCTGCTAAC GAAGCTGCTG CTGTAGCTGG TGCGGCTGGC 
GGAGCGGCCC AGTCCGCCGG AGGTGAAGGC TCAGCCCCGG CGCAGATGTG CGGTGAAGAG 
CACGCCACGG TGACAGCTGA GGAACTGACA CAGCACAAGA ACTACCTGAA GGCCATCAAA 
CGCCAGGAGA GAGACATGAA GGCGGCAGAG AAGAAATATC AGAAAAAGAG CGAGGATCTG 
ATCCAGAAAT ACTCCGACAC CTTCAAGGCC ATCAAGAAGA AGATCTCATT GAAGAAAACA 
GAGGGAGGGG AAAACTCCAC GGACAGCGGC GTGAAGAGCG AGCGTGTGCA GGACCATAAA 
GAAAAAATGC TGGCTGAGCT GCAGCTGCAG TGGAAGGAAC AGTGTGACCA TCTGAGGAAG 
AAGAAGGAGC AGTTTGCAAC AGAGAGGCTG GCCGTGCTGC TGGAGATGGC AACAGAGCGG 
CACAACAGTG AACTGAAGAT GCTGGAGAGT GAACCCAAAG ATAACAAGAA GAAAACCAAA 
TGCTCGGACA AAGCCAAGTT GAAAAGGGCG ATGAGCACAG AGCTGTTGGA TGGGCCTTGT 
TTGATGGCTT GTTCGCTGGA GGAGGAGGGT CTGAGCGGGG CCCTGGTCAC CATGGAGGGG 
TCGACAGGAA GAAGCACACA AGGACAAATG ATTGACATGT ACAAAGACTG TGACCAGTAA