LOC101071533 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101071533
Entrez Gene ID	101071533
Full Name	hexokinase-2-like
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003974558.2	XP_003974607.1	hexokinase-2-like
XM_003974558.3	XP_003974607.2	hexokinase-2-like

Pathways from BioSystems
Gene Ontology

The following LOC101071533 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101071533 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo13423	XM_003974558.2 Latest version!	Takifugu rubripes hexokinase-2-like (LOC101071533), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OTo60734	XM_003974558.3 Latest version!	Takifugu rubripes hexokinase-2-like (LOC101071533), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101071533 ( XM_003974558.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003974607.1 Takifugu rubripes hexokinase-2-like (LOC101071533), mRNA.

Price & Availability↑

CloneID	OTo13423
Clone ID Related Accession (Same CDS sequence)	XM_003974558.2
Accession Version	XM_003974558.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	hexokinase-2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071154.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003974558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCC CGAGTCTGCT GGCTAACTGC TGCGACGAAC TTCAAGATGA CTACGCGCAA 
GTGGACAAAT ATCTACAGCG TTTTCAGTTA TCTTATGGAG ATTTGATGGA GATATCGAAC 
AGATTCCGCC GAGAAATGGA CAAAGGCCTG TGCAGAGACA CCAACCCCAC TGCTGTTGTC 
AAGATGTTGC CCACATTTGT TCACTCCACG CCCGATAGGA CAGAGCATGG TGAATTCCTT 
GCCTTGGATT TGGGAGGATC TACTTTCAGA GTACTCTTAG TCAAAGTAAT GGCTGATGGT 
GAGCAAAAGG TCGAGATGGA AAACCAGATT TATGTAATTC CTGAACACCT CATGAAAGGC 
AACGGGGTGA AGTTATTCGA TCACATTGCT GACTGTTTGG CAAATTTCAT GGAGAAGATT 
GGCATGAAGG AGAAGAAACT CCCACTGGGT TTCACTTTCT CTTTTCCCTG TCAGCAGACC 
AAACTGGATG AGAGTATTTT GTTGTCTTGG ACCAAAGCGT TCAAGTCAAA TGGAGTCGAG 
GGAAAAGATG TGGTCAGCCT TCTGAGGGCA GCTATCAAAA AACGAGGAGA CTTTGATGTT 
GATGTCATGG CTGTGATCAA TGACACAGTG GGAGCCATGA TGACCTGTGG CTACGATGAT 
CAGCTCTGTG AAATTGGTCT CATTGTGGGA ACTGGGACTA ACGCCTGTTA CATGGAGCAG 
ATGAGGAACA TTGAGGTGCT GGACGGTGAT GAAGGGCGGA TGTGTATCAA TACTGAGTGG 
GGAGCCTTTG GAGACGATGG TACCCTGGAG GACCATCGCA CTGACTTTGA CAGAGAAATT 
GATGCAGGCT CTTTAAACCC TGGAAATCAA CTGTTTGAGA AAATGGTCAG CGGCATGTAC 
ATGGGAGAAC TTGTGCGTGT CATCCTGGTG AAAATGACCA AAGAGCAGCT GCTGTTCAAG 
GGCAAGACTA CTGTGGGACT CCTCACGACT GGGAGCGTTA AGACCAGTTA CATCTATACC 
ATTGACAACG ACAATGATGA GGAAGGTCTG GCAAGTGCTG AAAAGGTGCT CCGTGGACTT 
AACCTCAACC CCACAAGCGA GGACTGCCTC GCTGTCCGAA GGGTGTGCCA GATTGTCTCG 
ACACGTTCTG CACACTTGTG TGCTGCCACG CTTGTAGCAG TTCTGCGGCA GATCCGTGAT 
AACAAGGCAG CTGAGAAGCT GCGGACCACC ATTGGCGCAG ATGGATCTGT TTACAAGAAT 
CACCAAGAGT TTTCTCGGAG GCTGCACAAG ATGGTCCGGC AACTCATGCC AGACAGTGAT 
GTTCGTTTTT TGACGTCCCA GTGTGGTAGT GGGAAAGGTG CTGCCATGGT AACAGCAGTG 
GCGTACCGAT ATGCTGCTCA ACAAGCGGAG CGTCAGGCCG TCCTCAACAC TTTGCGTTTG 
AGTCGTCAAC AGCTGCTGGA AGTGAAGAGT CGCATGAGGG AGAGGATGTT GCAGGGCCTG 
TCGAAGCAAA CTCATGAGGA AGCGAGTGTC AAGATGCTTC CCACCTATGT CATATCCACC 
CCAAACGGAA CAGAGCAAGG TGACTTCTTA GTTGTGGACC TTGGAGGCTC CAAATTCCGC 
GTGTTGCTGG CGCAAATACA GAATGGAAAA AAACGAAATG AGACCCTGCA CCAGAAGATC 
TACAATATCC CGCAGGAGAC CATGCAGGGC ACAGGAAAGG AGCTTTTTGA CCATATAGTT 
GATTGCATTG CTGACTTCCT GGAGTTCATG GGCATGAGTG GAGCATCTTT GCCTCTGGGA 
TTCACTTTCT CATTCCCCTG TCATCAAAGC AGACTAGACC AGGGAATATT ACTGAAGTGG 
ACGAAGGGTT TTAAAGCCAG CGGCTGCGAG GGTCAAGACG TTACTATGTT GCTGAAAGAG 
GCTGTGTGTC GCAGGAAGGA ATTTGATTTG AACTTTGTGG CTGTGGTGAA CGACACAGTC 
GGAACCATGA TGACTTGCGG TTATGAGGAC CCCCGATGTG AGGTGGGACT CATCGTAGGC 
ACAGGGACCA ACGCCTGCTA CATGGAGGAG GTACAGAATA TCGACCTTGT GGATGGCGAC 
GAAGGCCGTA TGTGTGTGAA CATGGAGTGG GGGGCGTTTG GAGACGCCGG TGAACTAGAT 
GACTTCTGCA CACAGTTCGA TGCCTTTGTA GATGAATCGT CCACCAACCC TGGGAAACAG 
AGATATGAGA AGATGGTCAG CGGCATGTAC CTGGGGGAGA TCGTGAGAAA CATCCTGATG 
CATTTCACTG ACAGAGGCCT GCTGTTCAGA GGGAAAGTCT CTGAGAGACT CAAGATGCAT 
GGCATCTTTG CCACAAAGTT CCTGTCACAG ATCGAAAGTG ACCGCCTGGC CATGCGGCAG 
GTCCGAGCCA TTTTGCAGCA CTTGGGCCTG ACAAACTCCA CCTGTGACGA CAGCGTGCTG 
GTAAAGGAGG TGTGCGGCGT CGTGTCCCGG CGTGCAGCTC AGCTCTGCGG CGCCGGTTTA 
GCGGCCGTGG TGGATAAGAT GCGAGAGAAC CGCAATTTGA ATCAGCTTTC TGTCACCGTG 
GGAGTGGACG GCACCCTTTA CAAAACACAC CCCCATTTCT CTAGGATAAT GCAGGAGACT 
TTGCAGGATT TGGCGCCGCA GTGCCAGGTG ACGTTCCACA AGTCGGAGGA TGGCAGCGGG 
AAGGGCGCTG CCCTGATCAC GGCCGTGGCC TGCAGGTTGA GGAGGGAGGA AGAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003974607.1
CDS	213..2972
Translation	MSSPSLLANCCDELQDDYAQVDKYLQRFQLSYGDLMEISNRFRREMDKGLCRDTNPTAVVKMLPTFVHSTPDRTEHGEFLALDLGGSTFRVLLVKVMADGEQKVEMENQIYVIPEHLMKGNGVKLFDHIADCLANFMEKIGMKEKKLPLGFTFSFPCQQTKLDESILLSWTKAFKSNGVEGKDVVSLLRAAIKKRGDFDVDVMAVINDTVGAMMTCGYDDQLCEIGLIVGTGTNACYMEQMRNIEVLDGDEGRMCINTEWGAFGDDGTLEDHRTDFDREIDAGSLNPGNQLFEKMVSGMYMGELVRVILVKMTKEQLLFKGKTTVGLLTTGSVKTSYIYTIDNDNDEEGLASAEKVLRGLNLNPTSEDCLAVRRVCQIVSTRSAHLCAATLVAVLRQIRDNKAAEKLRTTIGADGSVYKNHQEFSRRLHKMVRQLMPDSDVRFLTSQCGSGKGAAMVTAVAYRYAAQQAERQAVLNTLRLSRQQLLEVKSRMRERMLQGLSKQTHEEASVKMLPTYVISTPNGTEQGDFLVVDLGGSKFRVLLAQIQNGKKRNETLHQKIYNIPQETMQGTGKELFDHIVDCIADFLEFMGMSGASLPLGFTFSFPCHQSRLDQGILLKWTKGFKASGCEGQDVTMLLKEAVCRRKEFDLNFVAVVNDTVGTMMTCGYEDPRCEVGLIVGTGTNACYMEEVQNIDLVDGDEGRMCVNMEWGAFGDAGELDDFCTQFDAFVDESSTNPGKQRYEKMVSGMYLGEIVRNILMHFTDRGLLFRGKVSERLKMHGIFATKFLSQIESDRLAMRQVRAILQHLGLTNSTCDDSVLVKEVCGVVSRRAAQLCGAGLAAVVDKMRENRNLNQLSVTVGVDGTLYKTHPHFSRIMQETLQDLAPQCQVTFHKSEDGSGKGAALITAVACRLRREEEQ

Target ORF information:

RefSeq Version	XM_003974558.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes hexokinase-2-like (LOC101071533), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003974558.2

ORF Insert Sequence:

ATGTCGTCCC CGAGTCTGCT GGCTAACTGC TGCGACGAAC TTCAAGATGA CTACGCGCAA 
GTGGACAAAT ATCTACAGCG TTTTCAGTTA TCTTATGGAG ATTTGATGGA GATATCGAAC 
AGATTCCGCC GAGAAATGGA CAAAGGCCTG TGCAGAGACA CCAACCCCAC TGCTGTTGTC 
AAGATGTTGC CCACATTTGT TCACTCCACG CCCGATAGGA CAGAGCATGG TGAATTCCTT 
GCCTTGGATT TGGGAGGATC TACTTTCAGA GTACTCTTAG TCAAAGTAAT GGCTGATGGT 
GAGCAAAAGG TCGAGATGGA AAACCAGATT TATGTAATTC CTGAACACCT CATGAAAGGC 
AACGGGGTGA AGTTATTCGA TCACATTGCT GACTGTTTGG CAAATTTCAT GGAGAAGATT 
GGCATGAAGG AGAAGAAACT CCCACTGGGT TTCACTTTCT CTTTTCCCTG TCAGCAGACC 
AAACTGGATG AGAGTATTTT GTTGTCTTGG ACCAAAGCGT TCAAGTCAAA TGGAGTCGAG 
GGAAAAGATG TGGTCAGCCT TCTGAGGGCA GCTATCAAAA AACGAGGAGA CTTTGATGTT 
GATGTCATGG CTGTGATCAA TGACACAGTG GGAGCCATGA TGACCTGTGG CTACGATGAT 
CAGCTCTGTG AAATTGGTCT CATTGTGGGA ACTGGGACTA ACGCCTGTTA CATGGAGCAG 
ATGAGGAACA TTGAGGTGCT GGACGGTGAT GAAGGGCGGA TGTGTATCAA TACTGAGTGG 
GGAGCCTTTG GAGACGATGG TACCCTGGAG GACCATCGCA CTGACTTTGA CAGAGAAATT 
GATGCAGGCT CTTTAAACCC TGGAAATCAA CTGTTTGAGA AAATGGTCAG CGGCATGTAC 
ATGGGAGAAC TTGTGCGTGT CATCCTGGTG AAAATGACCA AAGAGCAGCT GCTGTTCAAG 
GGCAAGACTA CTGTGGGACT CCTCACGACT GGGAGCGTTA AGACCAGTTA CATCTATACC 
ATTGACAACG ACAATGATGA GGAAGGTCTG GCAAGTGCTG AAAAGGTGCT CCGTGGACTT 
AACCTCAACC CCACAAGCGA GGACTGCCTC GCTGTCCGAA GGGTGTGCCA GATTGTCTCG 
ACACGTTCTG CACACTTGTG TGCTGCCACG CTTGTAGCAG TTCTGCGGCA GATCCGTGAT 
AACAAGGCAG CTGAGAAGCT GCGGACCACC ATTGGCGCAG ATGGATCTGT TTACAAGAAT 
CACCAAGAGT TTTCTCGGAG GCTGCACAAG ATGGTCCGGC AACTCATGCC AGACAGTGAT 
GTTCGTTTTT TGACGTCCCA GTGTGGTAGT GGGAAAGGTG CTGCCATGGT AACAGCAGTG 
GCGTACCGAT ATGCTGCTCA ACAAGCGGAG CGTCAGGCCG TCCTCAACAC TTTGCGTTTG 
AGTCGTCAAC AGCTGCTGGA AGTGAAGAGT CGCATGAGGG AGAGGATGTT GCAGGGCCTG 
TCGAAGCAAA CTCATGAGGA AGCGAGTGTC AAGATGCTTC CCACCTATGT CATATCCACC 
CCAAACGGAA CAGAGCAAGG TGACTTCTTA GTTGTGGACC TTGGAGGCTC CAAATTCCGC 
GTGTTGCTGG CGCAAATACA GAATGGAAAA AAACGAAATG AGACCCTGCA CCAGAAGATC 
TACAATATCC CGCAGGAGAC CATGCAGGGC ACAGGAAAGG AGCTTTTTGA CCATATAGTT 
GATTGCATTG CTGACTTCCT GGAGTTCATG GGCATGAGTG GAGCATCTTT GCCTCTGGGA 
TTCACTTTCT CATTCCCCTG TCATCAAAGC AGACTAGACC AGGGAATATT ACTGAAGTGG 
ACGAAGGGTT TTAAAGCCAG CGGCTGCGAG GGTCAAGACG TTACTATGTT GCTGAAAGAG 
GCTGTGTGTC GCAGGAAGGA ATTTGATTTG AACTTTGTGG CTGTGGTGAA CGACACAGTC 
GGAACCATGA TGACTTGCGG TTATGAGGAC CCCCGATGTG AGGTGGGACT CATCGTAGGC 
ACAGGGACCA ACGCCTGCTA CATGGAGGAG GTACAGAATA TCGACCTTGT GGATGGCGAC 
GAAGGCCGTA TGTGTGTGAA CATGGAGTGG GGGGCGTTTG GAGACGCCGG TGAACTAGAT 
GACTTCTGCA CACAGTTCGA TGCCTTTGTA GATGAATCGT CCACCAACCC TGGGAAACAG 
AGATATGAGA AGATGGTCAG CGGCATGTAC CTGGGGGAGA TCGTGAGAAA CATCCTGATG 
CATTTCACTG ACAGAGGCCT GCTGTTCAGA GGGAAAGTCT CTGAGAGACT CAAGATGCAT 
GGCATCTTTG CCACAAAGTT CCTGTCACAG ATCGAAAGTG ACCGCCTGGC CATGCGGCAG 
GTCCGAGCCA TTTTGCAGCA CTTGGGCCTG ACAAACTCCA CCTGTGACGA CAGCGTGCTG 
GTAAAGGAGG TGTGCGGCGT CGTGTCCCGG CGTGCAGCTC AGCTCTGCGG CGCCGGTTTA 
GCGGCCGTGG TGGATAAGAT GCGAGAGAAC CGCAATTTGA ATCAGCTTTC TGTCACCGTG 
GGAGTGGACG GCACCCTTTA CAAAACACAC CCCCATTTCT CTAGGATAAT GCAGGAGACT 
TTGCAGGATT TGGCGCCGCA GTGCCAGGTG ACGTTCCACA AGTCGGAGGA TGGCAGCGGG 
AAGGGCGCTG CCCTGATCAC GGCCGTGGCC TGCAGGTTGA GGAGGGAGGA AGAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101071533 ( XM_003974558.3 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003974607.2 Takifugu rubripes hexokinase-2-like (LOC101071533), mRNA.

Price & Availability↑

CloneID	OTo60734
Clone ID Related Accession (Same CDS sequence)	XM_003974558.3
Accession Version	XM_003974558.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	hexokinase-2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042305.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003974558.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCCC CGAGTCTGCT GGCTAACTGC TGCGACGAAC TTCAAGATGA CTACGCGCAA 
GTGGACAAAT ATCTACAGCG TTTTCAGTTA TCTTATGGAG ATTTGATGGA GATATCGAAC 
AGATTCCGAC GAGAAATGGA CAAAGGCCTG TGCAGAGACA CCAACCCCAC TGCTGTTGTC 
AAGATGTTGC CCACATTTGT TCACTCCACG CCCGATAGGA CAGAGCATGG TGAATTCCTT 
GCCTTGGATT TGGGAGGATC TACTTTCAGA GTACTCTTAG TCAAAGTAAT GGCTGATGGT 
GAGCAAAAGG TCGAGATGGA AAACCAGATT TATGTAATTC CTGAACACCT CATGAAAGGC 
AACGGGGTGA AGTTATTCGA TCACATTGCT GACTGTTTGG CAAATTTCAT GGAGAAGATT 
GGCATGAAGG AGAAGAAACT CCCACTGGGT TTCACTTTCT CTTTTCCCTG TCAGCAGACC 
AAACTGGATG AGAGTATTTT GTTGTCTTGG ACCAAAGCGT TCAAGTCAAA TGGAGTCGAG 
GGAAAAGATG TGGTCAGCCT TCTGAGGGCA GCTATCAAAA AACGAGGAGA CTTTGATGTT 
GATGTCATGG CTGTGATCAA TGACACAGTG GGAGCCATGA TGACCTGTGG CTACGATGAT 
CAGCTCTGTG AAATTGGTCT CATTGTGGGA ACTGGGACTA ACGCCTGTTA CATGGAGCAG 
ATGAGGAACA TTGAGGTGCT GGACGGTGAT GAAGGGCGGA TGTGTATCAA TACTGAGTGG 
GGAGCCTTTG GAGACGATGG TACCCTGGAG GACCATCGCA CTGACTTTGA CAGAGAAATT 
GATGCAGGCT CTTTAAACCC TGGAAATCAA CTGTTTGAGA AAATGGTCAG CGGCATGTAC 
ATGGGAGAAC TTGTGCGTGT CATCCTGGTG AAAATGACCA AAGAGCAGCT GCTGTTCAAG 
GGCAAGACTA CTGTGGGACT CCTCACGACT GGGAGCGTTA AGACCAGTTA CATCTATACC 
ATTGACAACG ACAATGATGA GGAAGGTCTG GCAAGTGCTG AAAAGGTGCT CCGTGGACTT 
AACCTCAACC CCACAAGCGA GGACTGCCTC GCTGTCCGAA GGGTGTGCCA GATTGTCTCG 
ACACGTTCTG CACACTTGTG TGCTGCCACG CTTGTAGCAG TTCTGCGGCA GATCCGTGAT 
AACAAGGCAG CTGAGAAGCT GCGGACCACC ATTGGCGCAG ATGGATCTGT TTACAAGAAT 
CACCAAGAGT TTTCTCGGAG GCTGCACAAG ATGGTCCGGC AACTCATGCC AGACAGTGAT 
GTTCGTTTTT TGACGTCCCA GTGTGGTAGT GGGAAAGGTG CTGCCATGGT AACAGCAGTG 
GCGTACCGAT ATGCTGCTCA ACAAGCGGAG CGTCAGGCCG TCCTCAACAC TTTGCGTTTG 
AGTCGTCAAC AGCTGCTGGA AGTGAAGAGT CGCATGAGGG AGAGGATGTT GCAGGGCCTG 
TCGAAGCAAA CTCATGAGGA AGCGAGTGTC AAGATGCTTC CCACCTATGT CATATCCACC 
CCAAACGGAA CAGAGCAAGG TGACTTCTTA GTTGTGGACC TTGGAGGCTC CAAATTCCGC 
GTGTTGCTGG CGCAAATACA GAATGGAAAA AAACGAAATG AGACCCTGCA CCAGAAGATC 
TACAATATCC CGCAGGAGAC CATGCAGGGC ACAGGAAAGG AGCTTTTTGA CCATATAGTT 
GATTGCATTG CTGACTTCCT GGAGTTCATG GGCATGAGTG GAGCATCTTT GCCTCTGGGA 
TTCACTTTCT CATTCCCCTG TCATCAAAGC AGACTAGACC AGGGAATATT ACTGAAGTGG 
ACGAAGGGTT TTAAAGCCAG CGGCTGCGAG GGTCAAGACA TTACTATGTT GCTGAAAGAG 
GCTGTGTGTC GCAGGAAGGA ATTTGATTTG AACTTTGTGG CTGTGGTGAA CGACACAGTC 
GGAACCATGA TGACTTGCGG TTATGAGGAC CCCCGATGTG AGGTGGGACT CATCGTAGGC 
ACAGGGACCA ACGCCTGCTA CATGGAGGAG GTACAGAATA TCGACCTTGT GGATGGCGAC 
GAAGGCCGTA TGTGTGTGAA CATGGAGTGG GGGGCGTTTG GAGACGCCGG TGAACTAGAT 
GACTTCTGCA CACAGTTCGA TGCCTTTGTA GATGAATCAT CCACCAACCC TGGGAAACAG 
AGATATGAGA AGATGGTCAG CGGCATGTAC CTGGGGGAGA TCGTGAGAAA CATCCTGATG 
CATTTCACTG ACAGAGGCCT GCTGTTCAGA GGGAAAGTCT CTGAGAGACT CAAGATGCAC 
GGCATCTTTG CCACAAAGTT CCTGTCACAG ATCGAAAGTG ACCGCCTGGC CATGCGGCAG 
GTCCGAGCCA TTCTGCAGCA CTTGGGCCTG ACAAACTCCA CCTGTGACGA CAGCGTGCTG 
GTAAAGGAGG TGTGCGGCGT CGTGTCCCGG CGTGCAGCTC AGCTCTGCGG CGCCGGTTTA 
GCGGCCGTGG TGGATAAGAT GCGAGAGAAC CGCAATTTGA ATCAGCTTTC TGTCACCGTG 
GGAGTGGACG GCACCCTTTA CAAAACACAC CCCCATTTCT CTAGGATAAT GCAGGAGACT 
TTGCAGGATT TGGCGCCGCA GTGCCAGGTG ACGTTCCACA AGTCGGAGGA TGGCAGCGGG 
AAGGGCGCTG CCCTGATCAC GGCCGTGGCC TGCAGGTTGA GGAGGGAGGA AGAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003974607.2
CDS	209..2968
Translation	MSSPSLLANCCDELQDDYAQVDKYLQRFQLSYGDLMEISNRFRREMDKGLCRDTNPTAVVKMLPTFVHSTPDRTEHGEFLALDLGGSTFRVLLVKVMADGEQKVEMENQIYVIPEHLMKGNGVKLFDHIADCLANFMEKIGMKEKKLPLGFTFSFPCQQTKLDESILLSWTKAFKSNGVEGKDVVSLLRAAIKKRGDFDVDVMAVINDTVGAMMTCGYDDQLCEIGLIVGTGTNACYMEQMRNIEVLDGDEGRMCINTEWGAFGDDGTLEDHRTDFDREIDAGSLNPGNQLFEKMVSGMYMGELVRVILVKMTKEQLLFKGKTTVGLLTTGSVKTSYIYTIDNDNDEEGLASAEKVLRGLNLNPTSEDCLAVRRVCQIVSTRSAHLCAATLVAVLRQIRDNKAAEKLRTTIGADGSVYKNHQEFSRRLHKMVRQLMPDSDVRFLTSQCGSGKGAAMVTAVAYRYAAQQAERQAVLNTLRLSRQQLLEVKSRMRERMLQGLSKQTHEEASVKMLPTYVISTPNGTEQGDFLVVDLGGSKFRVLLAQIQNGKKRNETLHQKIYNIPQETMQGTGKELFDHIVDCIADFLEFMGMSGASLPLGFTFSFPCHQSRLDQGILLKWTKGFKASGCEGQDITMLLKEAVCRRKEFDLNFVAVVNDTVGTMMTCGYEDPRCEVGLIVGTGTNACYMEEVQNIDLVDGDEGRMCVNMEWGAFGDAGELDDFCTQFDAFVDESSTNPGKQRYEKMVSGMYLGEIVRNILMHFTDRGLLFRGKVSERLKMHGIFATKFLSQIESDRLAMRQVRAILQHLGLTNSTCDDSVLVKEVCGVVSRRAAQLCGAGLAAVVDKMRENRNLNQLSVTVGVDGTLYKTHPHFSRIMQETLQDLAPQCQVTFHKSEDGSGKGAALITAVACRLRREEEQ

Target ORF information:

RefSeq Version	XM_003974558.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes hexokinase-2-like (LOC101071533), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003974558.3

ORF Insert Sequence:

ATGTCGTCCC CGAGTCTGCT GGCTAACTGC TGCGACGAAC TTCAAGATGA CTACGCGCAA 
GTGGACAAAT ATCTACAGCG TTTTCAGTTA TCTTATGGAG ATTTGATGGA GATATCGAAC 
AGATTCCGAC GAGAAATGGA CAAAGGCCTG TGCAGAGACA CCAACCCCAC TGCTGTTGTC 
AAGATGTTGC CCACATTTGT TCACTCCACG CCCGATAGGA CAGAGCATGG TGAATTCCTT 
GCCTTGGATT TGGGAGGATC TACTTTCAGA GTACTCTTAG TCAAAGTAAT GGCTGATGGT 
GAGCAAAAGG TCGAGATGGA AAACCAGATT TATGTAATTC CTGAACACCT CATGAAAGGC 
AACGGGGTGA AGTTATTCGA TCACATTGCT GACTGTTTGG CAAATTTCAT GGAGAAGATT 
GGCATGAAGG AGAAGAAACT CCCACTGGGT TTCACTTTCT CTTTTCCCTG TCAGCAGACC 
AAACTGGATG AGAGTATTTT GTTGTCTTGG ACCAAAGCGT TCAAGTCAAA TGGAGTCGAG 
GGAAAAGATG TGGTCAGCCT TCTGAGGGCA GCTATCAAAA AACGAGGAGA CTTTGATGTT 
GATGTCATGG CTGTGATCAA TGACACAGTG GGAGCCATGA TGACCTGTGG CTACGATGAT 
CAGCTCTGTG AAATTGGTCT CATTGTGGGA ACTGGGACTA ACGCCTGTTA CATGGAGCAG 
ATGAGGAACA TTGAGGTGCT GGACGGTGAT GAAGGGCGGA TGTGTATCAA TACTGAGTGG 
GGAGCCTTTG GAGACGATGG TACCCTGGAG GACCATCGCA CTGACTTTGA CAGAGAAATT 
GATGCAGGCT CTTTAAACCC TGGAAATCAA CTGTTTGAGA AAATGGTCAG CGGCATGTAC 
ATGGGAGAAC TTGTGCGTGT CATCCTGGTG AAAATGACCA AAGAGCAGCT GCTGTTCAAG 
GGCAAGACTA CTGTGGGACT CCTCACGACT GGGAGCGTTA AGACCAGTTA CATCTATACC 
ATTGACAACG ACAATGATGA GGAAGGTCTG GCAAGTGCTG AAAAGGTGCT CCGTGGACTT 
AACCTCAACC CCACAAGCGA GGACTGCCTC GCTGTCCGAA GGGTGTGCCA GATTGTCTCG 
ACACGTTCTG CACACTTGTG TGCTGCCACG CTTGTAGCAG TTCTGCGGCA GATCCGTGAT 
AACAAGGCAG CTGAGAAGCT GCGGACCACC ATTGGCGCAG ATGGATCTGT TTACAAGAAT 
CACCAAGAGT TTTCTCGGAG GCTGCACAAG ATGGTCCGGC AACTCATGCC AGACAGTGAT 
GTTCGTTTTT TGACGTCCCA GTGTGGTAGT GGGAAAGGTG CTGCCATGGT AACAGCAGTG 
GCGTACCGAT ATGCTGCTCA ACAAGCGGAG CGTCAGGCCG TCCTCAACAC TTTGCGTTTG 
AGTCGTCAAC AGCTGCTGGA AGTGAAGAGT CGCATGAGGG AGAGGATGTT GCAGGGCCTG 
TCGAAGCAAA CTCATGAGGA AGCGAGTGTC AAGATGCTTC CCACCTATGT CATATCCACC 
CCAAACGGAA CAGAGCAAGG TGACTTCTTA GTTGTGGACC TTGGAGGCTC CAAATTCCGC 
GTGTTGCTGG CGCAAATACA GAATGGAAAA AAACGAAATG AGACCCTGCA CCAGAAGATC 
TACAATATCC CGCAGGAGAC CATGCAGGGC ACAGGAAAGG AGCTTTTTGA CCATATAGTT 
GATTGCATTG CTGACTTCCT GGAGTTCATG GGCATGAGTG GAGCATCTTT GCCTCTGGGA 
TTCACTTTCT CATTCCCCTG TCATCAAAGC AGACTAGACC AGGGAATATT ACTGAAGTGG 
ACGAAGGGTT TTAAAGCCAG CGGCTGCGAG GGTCAAGACA TTACTATGTT GCTGAAAGAG 
GCTGTGTGTC GCAGGAAGGA ATTTGATTTG AACTTTGTGG CTGTGGTGAA CGACACAGTC 
GGAACCATGA TGACTTGCGG TTATGAGGAC CCCCGATGTG AGGTGGGACT CATCGTAGGC 
ACAGGGACCA ACGCCTGCTA CATGGAGGAG GTACAGAATA TCGACCTTGT GGATGGCGAC 
GAAGGCCGTA TGTGTGTGAA CATGGAGTGG GGGGCGTTTG GAGACGCCGG TGAACTAGAT 
GACTTCTGCA CACAGTTCGA TGCCTTTGTA GATGAATCAT CCACCAACCC TGGGAAACAG 
AGATATGAGA AGATGGTCAG CGGCATGTAC CTGGGGGAGA TCGTGAGAAA CATCCTGATG 
CATTTCACTG ACAGAGGCCT GCTGTTCAGA GGGAAAGTCT CTGAGAGACT CAAGATGCAC 
GGCATCTTTG CCACAAAGTT CCTGTCACAG ATCGAAAGTG ACCGCCTGGC CATGCGGCAG 
GTCCGAGCCA TTCTGCAGCA CTTGGGCCTG ACAAACTCCA CCTGTGACGA CAGCGTGCTG 
GTAAAGGAGG TGTGCGGCGT CGTGTCCCGG CGTGCAGCTC AGCTCTGCGG CGCCGGTTTA 
GCGGCCGTGG TGGATAAGAT GCGAGAGAAC CGCAATTTGA ATCAGCTTTC TGTCACCGTG 
GGAGTGGACG GCACCCTTTA CAAAACACAC CCCCATTTCT CTAGGATAAT GCAGGAGACT 
TTGCAGGATT TGGCGCCGCA GTGCCAGGTG ACGTTCCACA AGTCGGAGGA TGGCAGCGGG 
AAGGGCGCTG CCCTGATCAC GGCCGTGGCC TGCAGGTTGA GGAGGGAGGA AGAGCAATGA