pde2a cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	pde2a
Entrez Gene ID	101070163
Full Name	phosphodiesterase 2A
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029844123.1	XP_029699983.1	LOW QUALITY PROTEIN: cGMP-dependent 3',5'-cyclic phosphodiesterase
XM_011618097.1	XP_011616399.1	cGMP-dependent 3',5'-cyclic phosphodiesterase

Pathways from BioSystems
Gene Ontology

The following pde2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pde2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo25251	XM_011618097.1 Latest version!	Takifugu rubripes phosphodiesterase 2A, cGMP-stimulated (pde2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OTo45263	XM_029844123.1 Latest version!	Takifugu rubripes phosphodiesterase 2A (pde2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pde2a ( XM_011618097.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011616399.1 Takifugu rubripes phosphodiesterase 2A, cGMP-stimulated (pde2a), mRNA.

Price & Availability↑

CloneID	OTo25251
Clone ID Related Accession (Same CDS sequence)	XM_011618097.1
Accession Version	XM_011618097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	cGMP-dependent 3',5'-cyclic phosphodiesterase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071949.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGACCGCGC TCGAGATGAT GAAAGGGAAG GACCTCTGTG GTCAGCAAGT GTTTCCCCAT 
CCCGATGACA GTTCACCTTC AGCCCCGGCG ACCGCCGCGT TCTCCAAACA ACTCCAGGAT 
GCTTTGCTGC GGCTGGCGGC GGTCGTTGAC GTTCATTCGC TGCGAGCAGC TTTTCGAGAC 
AGCGTGCAGG ACCTCCTACC CCACACGGAA TGTGTGTGTG TCTACATGCT GGATGGAGAG 
TCAAGACTGA TTTGTGATGA GCCACCACAT GAACTGCCAA AGGAGGGAAA GATCAGGAGT 
ATCGTCAACC AACTGAATCG ACGTCAGTGT AATGGCCTTC CTCTGTCGGA GCTGCCAGAG 
AAATATCGAG CATGCCTGGC AGCACCGCTT CCAGCACACC GAAGAGCCAT TATCATTCCT 
ATCCTGGACC AGAAGCAAAG CGAGGCCATT GCCGTCTTAC TGGTTGGCTG TAATTCCCTC 
TCTGATCAGG ACGAAGTTCA CCTGAACACC CTGGAAAAAC ATACCCGCAT CTTCTCTACC 
ACCACGCATG CACCAGGCAT CGGTTGCATG TGTTCGCGTC CAGGCTGTCC AGGCATCCTA 
CCAGAGGCCT CCTCTCAGCC CTTCTTCCGT ACAGTCCATG ATGCACTGCT GCACCTCGAC 
ATCTCAGGCC ATGATTACAG CCAACTGGAC CGAGACATCC TCCAGCTTTG TGGTGAGCTC 
TTTGACCTCG ATGCTGCTTC TCTTCAGCTC AAAGTCATCA ATTATCTGCA GCAGCAGACG 
CAGTCGGAGT GCTGCTGTTT GCTGCTGGTG TCTGAGGACA ACCACCAGCT CTTTTGCCAG 
GTAGTTGGAG ACAAAGTCCT GGAGGAGGAG ATTAGTTTTC CGCTTACATT TGGACACTTC 
GGTCAGGTGG TGCAGAAAAA ACAGTCCATC ACTCTTCAAG ACATCACTGC TGAAGAGTGC 
AGGCAGATGT CCACCATGTT GGGATGCAAG ATCATGTCCA TGCTCTGCGT ACCAGTGGCG 
AGTAGAGCCA CCGGACAGGT GGTGGCGCTA GCATGTGCCT TTAACAAGCA GGGTGGGCAG 
AGGCATACAG AGGCAGATGA ACATGCCATC CAGCACTGCT TCTGTTACAC GTCAACGGTC 
CTCACTTCAA CTTTGGCCTT CCAGAAAGAA CAAAAACTGA AAGTGGAGTG CCAGGCACTC 
TTACAAGTGG CCAAGAACCT CTTTACACAC TTGGATGATG TGTCAGTGCT GTTACAGGAA 
ATTATAGTGG AGGCCAGGAA CCTAAGTGGT GCAGAGATCT GTTCCGTGTT CCTGCTGGAT 
CGACTCAGCC ACGAGCTGGT GGCAAAAGTG TTCGACGGCG GCGTCGTTAG TGATGAAGAG 
AAGGAGTTCC GTATCCCGGC AGATCAGGGG ATTGCGGGTC ACGTGGCCAC CACAGGTCAG 
ATCCTGAACA TTAAGGATGC CTACTCCCAC CCTCTCTTCT ACCGTGGAGT GGACGACAGC 
ACTGGCTTCA GGACCCGGAA CATCCTCTGC TTCCCCATCA AAGATGAGAC CAACGAGGTG 
ATCGGGGTGG CGGAGCTGGT GAATAAAATG AACGGGCCCT GGTTCAACCG TTTCGACGAG 
GACCTGGCCA CGGCGTTCTC CATCTACTGT GGCATCAGCA TCGCGCACTC TCTGCTGTAT 
AAGAGGGTCC ACGAAGCACA GTTCCGATCT CATTTGGCCA ACGAAATGAT GATGTACCAC 
ATGAAGGTTT CAGGGGAAGA GGTCTCTAAG CTGCTGGTGT CCGGGATTGA GCCAATCAAG 
GAGATCGATC CCTGCTTCGC GGAATTCACT TACACACCTC GCTCACTATC TGACGATTCC 
ACACCGATGT GTGTCCTCAG CATGTTTGAA GACATGGGCT TCATCAACAC GTACAAGATT 
GACCTGCACA CTCTCGCCAG GTTCTGTCTT ATGGTGAAGA AAGGCTACAG GGACCCTCCG 
TACCACAACT GGATGCACGC CTTCTCCGTC AGCCACTTTT GCTACCTGCT CTACAAAAAC 
CTTGGGCTCT CACGCTACCT CCAAGACATA GAGATCCTCG CGCTCTTCGT CTCCTGTATG 
TGCCACGACC TGGACCACCG CGGCACCAAC AACTCGTTCC AAGTTGCCTC GCAATCTGTG 
CTGGCTGCTC TCTACAGCTC CGAGGGATCC GTGATGGAGC GGCACCACTT CGCTCAGGCC 
ATCGCCATCT TGAACACTGT GGGCTGCAAC ATCTTCGAGA AGTTCTCCAG AAAGGACTAC 
CAGCGGATGT TGGACTTGAT ACGAGACATC ATTTTGGCGA CTGACCTGGC TCACCACCTT 
CGGATTTTCA AAGACTTGCA GAAGATGGCT GACGATGGAT ACAATCCCAA AAGCCAGACG 
CACCGCTCCA TGCTGCTGTG CCTGCTGATG ACATCGTGTG ACCTGTCAGA CCAAACCAAG 
GGCTGGAAAA CCACGCGCAA GATTGCCGAG CTGATCTATA AAGAGTTCTT CTCCCAAGGA 
GATCTGGAAA AAGCAATGGG CAACAGGCCC AGTGAAATGA TGGACAGAGA AAAAGCTTAC 
ATCCCAGAGC TGCAGATAAG CTTCATGGAA CACATCGCCA TGCCGATTTA CAAGCTTCTC 
TCGGAACTGT TTCCCGGCGC CGCCGAGCTG TACGAGAGAG TCGCCGCCAA CCGGGAGCAG 
TGGACCAAAG TGTCCCACAA GTTCACCATC CGCGGGTTGC CGAGCAACAA CAGTTTAGAT 
TTCCTGGATC AAGAATACGA ACTGCTGCAG TCAGAGGGCG CTCTGACCAG TGACGACCAA 
TGCCTAAACG GTTGCCTTGA CGATGCAGAG GGCGGGAGGT GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011616399.1
CDS	306..3173
Translation	MTALEMMKGKDLCGQQVFPHPDDSSPSAPATAAFSKQLQDALLRLAAVVDVHSLRAAFRDSVQDLLPHTECVCVYMLDGESRLICDEPPHELPKEGKIRSIVNQLNRRQCNGLPLSELPEKYRACLAAPLPAHRRAIIIPILDQKQSEAIAVLLVGCNSLSDQDEVHLNTLEKHTRIFSTTTHAPGIGCMCSRPGCPGILPEASSQPFFRTVHDALLHLDISGHDYSQLDRDILQLCGELFDLDAASLQLKVINYLQQQTQSECCCLLLVSEDNHQLFCQVVGDKVLEEEISFPLTFGHFGQVVQKKQSITLQDITAEECRQMSTMLGCKIMSMLCVPVASRATGQVVALACAFNKQGGQRHTEADEHAIQHCFCYTSTVLTSTLAFQKEQKLKVECQALLQVAKNLFTHLDDVSVLLQEIIVEARNLSGAEICSVFLLDRLSHELVAKVFDGGVVSDEEKEFRIPADQGIAGHVATTGQILNIKDAYSHPLFYRGVDDSTGFRTRNILCFPIKDETNEVIGVAELVNKMNGPWFNRFDEDLATAFSIYCGISIAHSLLYKRVHEAQFRSHLANEMMMYHMKVSGEEVSKLLVSGIEPIKEIDPCFAEFTYTPRSLSDDSTPMCVLSMFEDMGFINTYKIDLHTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLGLSRYLQDIEILALFVSCMCHDLDHRGTNNSFQVASQSVLAALYSSEGSVMERHHFAQAIAILNTVGCNIFEKFSRKDYQRMLDLIRDIILATDLAHHLRIFKDLQKMADDGYNPKSQTHRSMLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPSEMMDREKAYIPELQISFMEHIAMPIYKLLSELFPGAAELYERVAANREQWTKVSHKFTIRGLPSNNSLDFLDQEYELLQSEGALTSDDQCLNGCLDDAEGGRCQ

Target ORF information:

RefSeq Version	XM_011618097.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phosphodiesterase 2A, cGMP-stimulated (pde2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011618097.1

ORF Insert Sequence:

ATGACCGCGC TCGAGATGAT GAAAGGGAAG GACCTCTGTG GTCAGCAAGT GTTTCCCCAT 
CCCGATGACA GTTCACCTTC AGCCCCGGCG ACCGCCGCGT TCTCCAAACA ACTCCAGGAT 
GCTTTGCTGC GGCTGGCGGC GGTCGTTGAC GTTCATTCGC TGCGAGCAGC TTTTCGAGAC 
AGCGTGCAGG ACCTCCTACC CCACACGGAA TGTGTGTGTG TCTACATGCT GGATGGAGAG 
TCAAGACTGA TTTGTGATGA GCCACCACAT GAACTGCCAA AGGAGGGAAA GATCAGGAGT 
ATCGTCAACC AACTGAATCG ACGTCAGTGT AATGGCCTTC CTCTGTCGGA GCTGCCAGAG 
AAATATCGAG CATGCCTGGC AGCACCGCTT CCAGCACACC GAAGAGCCAT TATCATTCCT 
ATCCTGGACC AGAAGCAAAG CGAGGCCATT GCCGTCTTAC TGGTTGGCTG TAATTCCCTC 
TCTGATCAGG ACGAAGTTCA CCTGAACACC CTGGAAAAAC ATACCCGCAT CTTCTCTACC 
ACCACGCATG CACCAGGCAT CGGTTGCATG TGTTCGCGTC CAGGCTGTCC AGGCATCCTA 
CCAGAGGCCT CCTCTCAGCC CTTCTTCCGT ACAGTCCATG ATGCACTGCT GCACCTCGAC 
ATCTCAGGCC ATGATTACAG CCAACTGGAC CGAGACATCC TCCAGCTTTG TGGTGAGCTC 
TTTGACCTCG ATGCTGCTTC TCTTCAGCTC AAAGTCATCA ATTATCTGCA GCAGCAGACG 
CAGTCGGAGT GCTGCTGTTT GCTGCTGGTG TCTGAGGACA ACCACCAGCT CTTTTGCCAG 
GTAGTTGGAG ACAAAGTCCT GGAGGAGGAG ATTAGTTTTC CGCTTACATT TGGACACTTC 
GGTCAGGTGG TGCAGAAAAA ACAGTCCATC ACTCTTCAAG ACATCACTGC TGAAGAGTGC 
AGGCAGATGT CCACCATGTT GGGATGCAAG ATCATGTCCA TGCTCTGCGT ACCAGTGGCG 
AGTAGAGCCA CCGGACAGGT GGTGGCGCTA GCATGTGCCT TTAACAAGCA GGGTGGGCAG 
AGGCATACAG AGGCAGATGA ACATGCCATC CAGCACTGCT TCTGTTACAC GTCAACGGTC 
CTCACTTCAA CTTTGGCCTT CCAGAAAGAA CAAAAACTGA AAGTGGAGTG CCAGGCACTC 
TTACAAGTGG CCAAGAACCT CTTTACACAC TTGGATGATG TGTCAGTGCT GTTACAGGAA 
ATTATAGTGG AGGCCAGGAA CCTAAGTGGT GCAGAGATCT GTTCCGTGTT CCTGCTGGAT 
CGACTCAGCC ACGAGCTGGT GGCAAAAGTG TTCGACGGCG GCGTCGTTAG TGATGAAGAG 
AAGGAGTTCC GTATCCCGGC AGATCAGGGG ATTGCGGGTC ACGTGGCCAC CACAGGTCAG 
ATCCTGAACA TTAAGGATGC CTACTCCCAC CCTCTCTTCT ACCGTGGAGT GGACGACAGC 
ACTGGCTTCA GGACCCGGAA CATCCTCTGC TTCCCCATCA AAGATGAGAC CAACGAGGTG 
ATCGGGGTGG CGGAGCTGGT GAATAAAATG AACGGGCCCT GGTTCAACCG TTTCGACGAG 
GACCTGGCCA CGGCGTTCTC CATCTACTGT GGCATCAGCA TCGCGCACTC TCTGCTGTAT 
AAGAGGGTCC ACGAAGCACA GTTCCGATCT CATTTGGCCA ACGAAATGAT GATGTACCAC 
ATGAAGGTTT CAGGGGAAGA GGTCTCTAAG CTGCTGGTGT CCGGGATTGA GCCAATCAAG 
GAGATCGATC CCTGCTTCGC GGAATTCACT TACACACCTC GCTCACTATC TGACGATTCC 
ACACCGATGT GTGTCCTCAG CATGTTTGAA GACATGGGCT TCATCAACAC GTACAAGATT 
GACCTGCACA CTCTCGCCAG GTTCTGTCTT ATGGTGAAGA AAGGCTACAG GGACCCTCCG 
TACCACAACT GGATGCACGC CTTCTCCGTC AGCCACTTTT GCTACCTGCT CTACAAAAAC 
CTTGGGCTCT CACGCTACCT CCAAGACATA GAGATCCTCG CGCTCTTCGT CTCCTGTATG 
TGCCACGACC TGGACCACCG CGGCACCAAC AACTCGTTCC AAGTTGCCTC GCAATCTGTG 
CTGGCTGCTC TCTACAGCTC CGAGGGATCC GTGATGGAGC GGCACCACTT CGCTCAGGCC 
ATCGCCATCT TGAACACTGT GGGCTGCAAC ATCTTCGAGA AGTTCTCCAG AAAGGACTAC 
CAGCGGATGT TGGACTTGAT ACGAGACATC ATTTTGGCGA CTGACCTGGC TCACCACCTT 
CGGATTTTCA AAGACTTGCA GAAGATGGCT GACGATGGAT ACAATCCCAA AAGCCAGACG 
CACCGCTCCA TGCTGCTGTG CCTGCTGATG ACATCGTGTG ACCTGTCAGA CCAAACCAAG 
GGCTGGAAAA CCACGCGCAA GATTGCCGAG CTGATCTATA AAGAGTTCTT CTCCCAAGGA 
GATCTGGAAA AAGCAATGGG CAACAGGCCC AGTGAAATGA TGGACAGAGA AAAAGCTTAC 
ATCCCAGAGC TGCAGATAAG CTTCATGGAA CACATCGCCA TGCCGATTTA CAAGCTTCTC 
TCGGAACTGT TTCCCGGCGC CGCCGAGCTG TACGAGAGAG TCGCCGCCAA CCGGGAGCAG 
TGGACCAAAG TGTCCCACAA GTTCACCATC CGCGGGTTGC CGAGCAACAA CAGTTTAGAT 
TTCCTGGATC AAGAATACGA ACTGCTGCAG TCAGAGGGCG CTCTGACCAG TGACGACCAA 
TGCCTAAACG GTTGCCTTGA CGATGCAGAG GGCGGGAGGT GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde2a ( XM_029844123.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029699983.1 Takifugu rubripes phosphodiesterase 2A (pde2a), mRNA.

Price & Availability↑

CloneID	OTo45263
Clone ID Related Accession (Same CDS sequence)	XM_029844123.1
Accession Version	XM_029844123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	LOW QUALITY PROTEIN: cGMP-dependent 3',5'-cyclic phosphodiesterase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042295.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGGGACAAG CGTGCGGACA CGCGCTCCTC TGCCGTAGCT ACCCGCCATC CTCCGAGATC 
TGTGGTCAGC AAGTGTTTCC CCATCCCGAT GACAGTTCAC CTTCAGCCCC GGCGACCGCC 
GCGTTCTCCA AACAACTCCA GGATGCTTTG CTGCGGCTGG CGGCGGTCGT TGACGTTCAT 
TCGCTGCGAG CAGCTTTTCG AGACGCGATG CAGGACCTCC TACCCCACAC GGAATGTGTG 
TGTGTCTACA TGCTGGATGG AGAGTCAAGG ACTTTTGGTG ATGAGCCACC ACATGAACTG 
CCAAAGGAGG GAAAGATCAG GAGTATCGTC AACCAACTGA ATCGACGTCA GTGTAATGGC 
CTTCCTCTGT CGGAGCTGCC AGAGAAATAT CGAGCATGCC TGGCAGCGCC GCTTCCAGCA 
CACCGAAGAG CCATTATCAT TCCTATCCTG GACCAGAAGC AAAGCGAGGC CATTGCCGTC 
TTACTGGTTG GCTGTAATTC ACTCTCTGAT CAGGACGAAG TTCACCCTGA ACACCCTGGA 
AAAACATGCA AGTCTTCCCT CCTTTACATT GGTTGCGCAT CGGTTGCATG TGTTCGCGTC 
CAGGCTGTCC AGGCATCCTA CCAGAGGCCT CCTCTCAGCC CTTCTTCCGT ACAGTCCCAT 
GATGCACTGC TGCACCTCGA CATCTCAGGC CATGATTACA GCCAACTGGA CCGAGACATC 
CTCCAGCTTT GTGGTGAGCT CTTTGACCTC GATGCTGCTT CTCTTCAGCT CAAAGTCATC 
AATTATCTGC AGCAGCAGAC GCAGTCGGAG TGCTGCTGTT TGCTGCTGGT GTCTGAGGAC 
AACCACCAGC TCTTTTGCCA GGTAGTTGGA GACAAAGTCC TGGAGGAGGA GATTAGTTTT 
CCGCTTACAT TTGGACACTT CGGTCAGGTG GTGCAGAAAA AACAGTCCAT CACTCTTCAA 
GACATCACTG CTGAAGAGTG CAGGCAGATG TCCACCATGT TGGGATGCAA GATCATGTCC 
ATGCTCTGCG TACCAGTGGC GAGTAGAGCC ACCGGACAGG TGGTGGCGCT AGCATGTGCC 
TTTAACAAGC AGGGTGGGCA GAGGCATACA GAGGCAGATG AACATGCCAT CCAGCACTGC 
TTCTGTTACA CTTCAACAGT CCTCACTTCA ACTTTGGCCT TCCAGAAAGA ACAGAAACTG 
AAAGTGGAGT GCCAGGCACT CTTACAAGTG GCCAAGAACC TCTTTACACA CTTGGATGAT 
GTGTCAGTGC TGTTACAGGA AATTATAGTG GAGGCCAGGA ACCTAAGTGG TGCAGAGATC 
TGTTCCGTGT TCCTGCTGGA TCGACTCAGC CACGAGCTGG TGGCAAAAGT GTTCGACGGC 
GGCGTCGTTA GTGATGAAGA GAAGGAGTTC CGTATCCCGG CAGATCAGGG GATTGCGGGT 
CACGTGGCCA CCACAGGTCA GATCCTGAAC ATTAAGGATG CCTACTCCCA CCCTCTCTTC 
TACCGTGGAG TGGACGACAG CACTGGCTTC AGGACCCGGA ACATCCTCTG CTTCCCCATC 
AAAGATGAGA CCAACGAGGT GATCGGGGTG GCGGAGCTGG TGAATAAAAT GAACGGGCCC 
TGGTTCAACC GTTTCGACGA GGACCTGGCC ACGGCGTTCT CCATCTACTG TGGCATCAGC 
ATCGCGCACT CTCTGCTGTA TAAGAGGGTC CACGAAGCAC AGTTCCGATC TCATTTGGCC 
AACGAAATGA TGATGTACCA CATGAAGGTT TCAGGGGAAG AGGTCTCTAA GCTGCTGGTG 
TCCGGGATTG AGCCAATCAA GGAGATCGAT CCCTGCTTCG CGGAATTCAC TTACACACCT 
CGCTCACTAT CTGACGATTC CACACCGATG TGTGTCCTCA GCATGTTTGA AGACATGGGC 
TTCATCAACA CGTACAAGAT TGACCTGCAC ACTCTCGCCA GGTTCTGTCT TATGGTGAAG 
AAAGGCTACA GGGACCCTCC GTACCACAAC TGGATGCACG CCTTCTCCGT CAGCCACTTT 
TGCTACCTGC TCTACAAAAA CCTTGGGCTC TCACGCTACC TCCAAGACAT AGAGATCCTC 
GCGCTCTTCG TCTCCTGTAT GTGCCACGAC CTGGACCACC GCGGCACCAA CAACTCGTTC 
CAAGTTGCCT CGCAATCTGT GCTGGCTGCT CTCTACAGCT CCGAGGGATC CGTGATGGAG 
CGGCACCACT TCGCTCAGGC CATCGCCATC TTGAACACTG TGGGCTGCAA CATCTTCGAG 
AAGTTCTCCA GAAAGGACTA CCAGCGGATG TTGGACTTGA TACGAGACAT CATTTTGGCG 
ACTGACCTGG CTCACCACCT TCGGATTTTC AAAGACTTGC AGAAGATGGC TGACGATGGA 
TACAATCCCA AAAGCCAGAC GCACCGCTCC ATGCTGCTGT GCCTGCTGAT GACATCGTGT 
GACCTGTCAG ACCAAACCAA GGGCTGGAAA ACCACGCGCA AGATTGCCGA GCTGATCTAT 
AAAGAGTTCT TCTCCCAAGG AGATCTGGAA AAAGCAATGG GCAACAGGCC CAGTGAAATG 
ATGGACAGAG AAAAAGCTTA CATCCCAGAG CTGCAGATAA GCTTCATGGA ACACATCGCC 
ATGCCGATTT ACAAGCTTCT CTCGGAACTG TTTCCCGGCG CCGCCGAGCT GTACGAGAGA 
GTCGCCGCCA ACCGGGAGCA GTGGACCAAA GTGTCCCACA AGTTCACCAT CCGCGGGTTG 
CCGAGCAACA ACAGTTTAGA TTTCCTGGAT CAAGAATACG AACTGCTGCA GTCAGAGGGC 
GCTCTGACCA GTGACGACCA ATGCCTAAAC GGTTGCCTTG ACGATGCAGA GGGCGGGAGG 
TGTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029699983.1
CDS	244..3132
Translation	MGQACGHALLCRSYPPSSEICGQQVFPHPDDSSPSAPATAAFSKQLQDALLRLAAVVDVHSLRAAFRDAMQDLLPHTECVCVYMLDGESRTFGDEPPHELPKEGKIRSIVNQLNRRQCNGLPLSELPEKYRACLAAPLPAHRRAIIIPILDQKQSEAIAVLLVGCNSLSDQDEVHPEHPGKTCKSSLLYIGCASVACVRVQAVQASYQRPPLSPSSVQSHDALLHLDISGHDYSQLDRDILQLCGELFDLDAASLQLKVINYLQQQTQSECCCLLLVSEDNHQLFCQVVGDKVLEEEISFPLTFGHFGQVVQKKQSITLQDITAEECRQMSTMLGCKIMSMLCVPVASRATGQVVALACAFNKQGGQRHTEADEHAIQHCFCYTSTVLTSTLAFQKEQKLKVECQALLQVAKNLFTHLDDVSVLLQEIIVEARNLSGAEICSVFLLDRLSHELVAKVFDGGVVSDEEKEFRIPADQGIAGHVATTGQILNIKDAYSHPLFYRGVDDSTGFRTRNILCFPIKDETNEVIGVAELVNKMNGPWFNRFDEDLATAFSIYCGISIAHSLLYKRVHEAQFRSHLANEMMMYHMKVSGEEVSKLLVSGIEPIKEIDPCFAEFTYTPRSLSDDSTPMCVLSMFEDMGFINTYKIDLHTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLGLSRYLQDIEILALFVSCMCHDLDHRGTNNSFQVASQSVLAALYSSEGSVMERHHFAQAIAILNTVGCNIFEKFSRKDYQRMLDLIRDIILATDLAHHLRIFKDLQKMADDGYNPKSQTHRSMLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPSEMMDREKAYIPELQISFMEHIAMPIYKLLSELFPGAAELYERVAANREQWTKVSHKFTIRGLPSNNSLDFLDQEYELLQSEGALTSDDQCLNGCLDDAEGGRCQ

Target ORF information:

RefSeq Version	XM_029844123.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphodiesterase 2A (pde2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029844123.1

ORF Insert Sequence:

ATGGGACAAG CGTGCGGACA CGCGCTCCTC TGCCGTAGCT ACCCGCCATC CTCCGAGATC 
TGTGGTCAGC AAGTGTTTCC CCATCCCGAT GACAGTTCAC CTTCAGCCCC GGCGACCGCC 
GCGTTCTCCA AACAACTCCA GGATGCTTTG CTGCGGCTGG CGGCGGTCGT TGACGTTCAT 
TCGCTGCGAG CAGCTTTTCG AGACGCGATG CAGGACCTCC TACCCCACAC GGAATGTGTG 
TGTGTCTACA TGCTGGATGG AGAGTCAAGG ACTTTTGGTG ATGAGCCACC ACATGAACTG 
CCAAAGGAGG GAAAGATCAG GAGTATCGTC AACCAACTGA ATCGACGTCA GTGTAATGGC 
CTTCCTCTGT CGGAGCTGCC AGAGAAATAT CGAGCATGCC TGGCAGCGCC GCTTCCAGCA 
CACCGAAGAG CCATTATCAT TCCTATCCTG GACCAGAAGC AAAGCGAGGC CATTGCCGTC 
TTACTGGTTG GCTGTAATTC ACTCTCTGAT CAGGACGAAG TTCACCCTGA ACACCCTGGA 
AAAACATGCA AGTCTTCCCT CCTTTACATT GGTTGCGCAT CGGTTGCATG TGTTCGCGTC 
CAGGCTGTCC AGGCATCCTA CCAGAGGCCT CCTCTCAGCC CTTCTTCCGT ACAGTCCCAT 
GATGCACTGC TGCACCTCGA CATCTCAGGC CATGATTACA GCCAACTGGA CCGAGACATC 
CTCCAGCTTT GTGGTGAGCT CTTTGACCTC GATGCTGCTT CTCTTCAGCT CAAAGTCATC 
AATTATCTGC AGCAGCAGAC GCAGTCGGAG TGCTGCTGTT TGCTGCTGGT GTCTGAGGAC 
AACCACCAGC TCTTTTGCCA GGTAGTTGGA GACAAAGTCC TGGAGGAGGA GATTAGTTTT 
CCGCTTACAT TTGGACACTT CGGTCAGGTG GTGCAGAAAA AACAGTCCAT CACTCTTCAA 
GACATCACTG CTGAAGAGTG CAGGCAGATG TCCACCATGT TGGGATGCAA GATCATGTCC 
ATGCTCTGCG TACCAGTGGC GAGTAGAGCC ACCGGACAGG TGGTGGCGCT AGCATGTGCC 
TTTAACAAGC AGGGTGGGCA GAGGCATACA GAGGCAGATG AACATGCCAT CCAGCACTGC 
TTCTGTTACA CTTCAACAGT CCTCACTTCA ACTTTGGCCT TCCAGAAAGA ACAGAAACTG 
AAAGTGGAGT GCCAGGCACT CTTACAAGTG GCCAAGAACC TCTTTACACA CTTGGATGAT 
GTGTCAGTGC TGTTACAGGA AATTATAGTG GAGGCCAGGA ACCTAAGTGG TGCAGAGATC 
TGTTCCGTGT TCCTGCTGGA TCGACTCAGC CACGAGCTGG TGGCAAAAGT GTTCGACGGC 
GGCGTCGTTA GTGATGAAGA GAAGGAGTTC CGTATCCCGG CAGATCAGGG GATTGCGGGT 
CACGTGGCCA CCACAGGTCA GATCCTGAAC ATTAAGGATG CCTACTCCCA CCCTCTCTTC 
TACCGTGGAG TGGACGACAG CACTGGCTTC AGGACCCGGA ACATCCTCTG CTTCCCCATC 
AAAGATGAGA CCAACGAGGT GATCGGGGTG GCGGAGCTGG TGAATAAAAT GAACGGGCCC 
TGGTTCAACC GTTTCGACGA GGACCTGGCC ACGGCGTTCT CCATCTACTG TGGCATCAGC 
ATCGCGCACT CTCTGCTGTA TAAGAGGGTC CACGAAGCAC AGTTCCGATC TCATTTGGCC 
AACGAAATGA TGATGTACCA CATGAAGGTT TCAGGGGAAG AGGTCTCTAA GCTGCTGGTG 
TCCGGGATTG AGCCAATCAA GGAGATCGAT CCCTGCTTCG CGGAATTCAC TTACACACCT 
CGCTCACTAT CTGACGATTC CACACCGATG TGTGTCCTCA GCATGTTTGA AGACATGGGC 
TTCATCAACA CGTACAAGAT TGACCTGCAC ACTCTCGCCA GGTTCTGTCT TATGGTGAAG 
AAAGGCTACA GGGACCCTCC GTACCACAAC TGGATGCACG CCTTCTCCGT CAGCCACTTT 
TGCTACCTGC TCTACAAAAA CCTTGGGCTC TCACGCTACC TCCAAGACAT AGAGATCCTC 
GCGCTCTTCG TCTCCTGTAT GTGCCACGAC CTGGACCACC GCGGCACCAA CAACTCGTTC 
CAAGTTGCCT CGCAATCTGT GCTGGCTGCT CTCTACAGCT CCGAGGGATC CGTGATGGAG 
CGGCACCACT TCGCTCAGGC CATCGCCATC TTGAACACTG TGGGCTGCAA CATCTTCGAG 
AAGTTCTCCA GAAAGGACTA CCAGCGGATG TTGGACTTGA TACGAGACAT CATTTTGGCG 
ACTGACCTGG CTCACCACCT TCGGATTTTC AAAGACTTGC AGAAGATGGC TGACGATGGA 
TACAATCCCA AAAGCCAGAC GCACCGCTCC ATGCTGCTGT GCCTGCTGAT GACATCGTGT 
GACCTGTCAG ACCAAACCAA GGGCTGGAAA ACCACGCGCA AGATTGCCGA GCTGATCTAT 
AAAGAGTTCT TCTCCCAAGG AGATCTGGAA AAAGCAATGG GCAACAGGCC CAGTGAAATG 
ATGGACAGAG AAAAAGCTTA CATCCCAGAG CTGCAGATAA GCTTCATGGA ACACATCGCC 
ATGCCGATTT ACAAGCTTCT CTCGGAACTG TTTCCCGGCG CCGCCGAGCT GTACGAGAGA 
GTCGCCGCCA ACCGGGAGCA GTGGACCAAA GTGTCCCACA AGTTCACCAT CCGCGGGTTG 
CCGAGCAACA ACAGTTTAGA TTTCCTGGAT CAAGAATACG AACTGCTGCA GTCAGAGGGC 
GCTCTGACCA GTGACGACCA ATGCCTAAAC GGTTGCCTTG ACGATGCAGA GGGCGGGAGG 
TGTCAGTGA