ogdh cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	ogdh
Entrez Gene ID	101068525
Full Name	oxoglutarate dehydrogenase
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029837362.1	XP_029693222.1	2-oxoglutarate dehydrogenase, mitochondrial
XM_011604775.1	XP_011603077.1	LOW QUALITY PROTEIN: 2-oxoglutarate dehydrogenase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following ogdh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ogdh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo24135	XM_011604775.1 Latest version!	Takifugu rubripes oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (ogdh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo67738	XM_029837362.1 Latest version!	Takifugu rubripes 2-oxoglutarate dehydrogenase, mitochondrial (LOC101068525), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ogdh ( XM_011604775.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011603077.1 Takifugu rubripes oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (ogdh), mRNA.

Price & Availability↑

CloneID	OTo24135
Clone ID Related Accession (Same CDS sequence)	XM_011604775.1
Accession Version	XM_011604775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	LOW QUALITY PROTEIN: 2-oxoglutarate dehydrogenase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070972.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCATCATT TAAGGACTAC CTTTCTGAGG CTTCGGCCTT TAACCGGTGC ACAGGCAGCT 
CAGAGATTGT CACAACCGCG CCTGTCGGTG ACCACAGCGG GCCCACGGAG GGTTCCGCCC 
TCCAGACAAT TGAACACGTC GGAGCCTTTT CTGAACGGCA CCAGCACCAA CTACATGGAG 
GAGATGTATT ATGCATGGCT GGAAAATCCA AAGAATGTCC ACAAGTCTTG GGATGTGTTT 
TTCCGTAACG TCAACGCCGG GGTTCCACCT GGGGCTGCTT ACCAGAGCCC CTCGTCCCTC 
CTCAAGCCTT CTCAGGGACT GACCGGTGCC CAGACACTTG TGGGGGCTCA GCCCAATATC 
GAGGAGCTGG TGGCGGATCA CTTAGCCGTG CAATCCCTCG TTCGAGCATA CCAGGTACGA 
GGGCACCACA TAGCAAAGTT AGACCCTCTG GAGATCAGCT GTGTGGACTT TGATGATGCA 
CCATGCGCCA TTGGCTTCCA GAACATTGGT TTTAATGGCC TGAACGAATC CCATTTGGAC 
AAAGTATTCC GGTTGCCAAA AACCACTTAC ATCGGAGGGG GTGAGAGTGC CCTGCCTCTC 
AGAGAGATTA TACGTCGGCT AGAGATGGCC TACTGTCAGC ACATTGGTGT GGAGTTCATG 
TTCATCAATG ACGTGCAGCA GTGCCATTGG ATCAGAGAAA AGTTTGAGAC ACCTGGAATC 
ATGAAGTTCA GTCTCGAGGA GAAGAGGACT CTTTTAGGCC GGGTGATCAG ATCCACAAGG 
TTTGAGGAGT TCCTCCAGAA GAAGTGGTCT TCAGAGAAAC GATTCGGCCT GGAAGGATGT 
GAATCCCTCA TCCCTGCCCT CAAGACAATC ATTGACATTT CCAGTCAGAG CGGAGTGGAG 
AGCATTATCA TGGGAATGCC TCACAGGGGA AGACTGAATG TGCTGGCCAA TGTCATTCGC 
AAGGAACTCG ACCAGATCTT CTGCCAGNTT GACTCCAAGC TTGAGGCTGC AGATGAGGGC 
TCTGGCGATG TGAAATATCA CTTGGGGATG TATCACAGGT GGTTGAACCG AGTCAGCAAC 
AAGTACATCA CAATGTCACT CATGGCGAAC CCCTCCCACC TGGAAGCCGT GGATCCGGTG 
GTGCAGGGCA AGACGAAAGC GGAGCAGTTC TACAGCGGAG ACGCAGAGGG CAAGAGGGTG 
ATGTCCATTC TGCTGCACGG TGATGCTGCG TTCGCAGGCC AGGGGATCGT TTACGAGACC 
TTCCACCTGT CTGACCTACC CTCCTACACC ACACACGGCA CAATACACGT GGTGGTCAAC 
AACCAGATCG GCTTCACCAC TGATCCCAGA ATGGCGCGCT CCTCCCCGTA CCCCACAGAT 
GTGGCTCGAG TCGTCAACGC TCCCATCTTC CACGTCAATG CCGATGACCC AGAGGCCGTG 
ATGTATGTGT GTAAGGTGGC AGCTGAATGG AGGAACACGT TCCATAAAGA TGTGGTTGTA 
GACCTGGTGT GCTACAGACG TAACGGCCAT AACGAGATGG ATGAGCCCAT GTTCACCCAG 
CCGCTGATGT ACAAGAAGAT CAAGAAGCAA AAGGGCGTCC TGAAAATTTA TGTAGAAAAG 
CTCATTGCTG AGGGAGTCAT CACACTACAA GAGTATCAGG AGGAAGTAGC AAAGTATGAC 
AAAATCTGCG AGGATGCGTA CAGTCGCTCC AAAGATGAAA AGATCCTTCA CATCAAACAC 
TGGCTGGACT CGCCGTGGCC CGATTTTTTT ACACTGGATG GACAACCAAA AACAATGAGC 
TGTAACTCCA CCGGCATCCC CGAAGAACAG CTCTGTCACA TCGGAAGCAT TGCTTCCTCG 
GTCCCACTAA AAGACTTCAC CATACACGGA GGTTTGACTC GTATCTTGAA GAGCCGTGCA 
AACATGGTGA GTCAGCGTGT GTGCGACTGG GCTCTGGGGG AATATATGGC CTTTGGCTCT 
CTGCTCCAGG ATGGAATCCA TGTACGTCTC AGTGGTCAAG ATGTGGAGAG GGGCACGTTT 
AGCCACCGGC ATCACGTTCT TCATGACCAG AACGTTGATA AGAGGATCTG CATCCCGATG 
AACCACATCT CCACCAGTCA AGCTCCTTAC ACCGTTTGCA ACAGCTCGCT GTCTGAGTAC 
GGAGTCTTAG GTTTTGAATT AGGCTTCGCT ATGGCCAGTC CAAATGCACT GGTTCTGTGG 
GAGGCCCAGT TTGGAGATTT TCACAACACA GCTCAGTGCA TCATCGACCA GTTCATCAGT 
CCGGGTCAGG CCAAATGGGT TCGACAGAAT GGCATCGTCC TGCTGCTTCC TCACGGCATG 
GAGGGAATGG GTCCAGAACA CTCATCTGCC CGGCCTGAAA GATTTCTACA GATGTGCAAC 
GATGACCCAG ATGCATCTCC TATTGTGACC GAAGACTTCG CAGTGCATCA GTTGTTCGAC 
TGTAACTGGA TTGTTGTCAA CTGCTCCACG CCCGCGAACT ACTTCCATGT TCTCCGCAGA 
CAGATCCTTC TGCCCTTCAG GAAGCCCCTG ATCGTGTTTA CTCCCAAGTC ACTGTTGCGC 
CACCCAGAGG CCAAATCCAG CTTTGATGAC ATGCTGCCAG GCACACACTT TAAGCGGATA 
ATCCCAGATG ACGGGCCCAC AACTGTCGAC CCACAAAAGG TGAAGAGAGT GGTTTTCTGC 
ACCGGCAAGG TCTACTATGA GCTGATCCGA GAACGCAAGA ACAGAGGCAT GGACGATGCC 
GTCGCGGTCG TGCGCATCGA GCAGCTCTCT CCATTCCCCT TTGATCTGGT GAAGGCTGAA 
ATCGATCAGT ACATCAATGC CGACCTGGTG TGGTGCCAGG AGGAGCACAA GAACCAGGGT 
TACTACGACT ACGTCAAGCC CCGCATCTCC AACACCACCC AGCACCGCCG CCCCGTCTGG 
TACGTTGGTC GTGAGCCTGC AGCGGCTCCC GCTACTGGAA ACAAACACAC CCATCTGGTG 
GAGCTCCAGC ATTTCCTGGA CACGGCCTTT GACCTGGAGG CATTCTCAGG AAGACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011603077.1
CDS	464..3523
Translation	MHHLRTTFLRLRPLTGAQAAQRLSQPRLSVTTAGPRRVPPSRQLNTSEPFLNGTSTNYMEEMYYAWLENPKNVHKSWDVFFRNVNAGVPPGAAYQSPSSLLKPSQGLTGAQTLVGAQPNIEELVADHLAVQSLVRAYQVRGHHIAKLDPLEISCVDFDDAPCAIGFQNIGFNGLNESHLDKVFRLPKTTYIGGGESALPLREIIRRLEMAYCQHIGVEFMFINDVQQCHWIREKFETPGIMKFSLEEKRTLLGRVIRSTRFEEFLQKKWSSEKRFGLEGCESLIPALKTIIDISSQSGVESIIMGMPHRGRLNVLANVIRKELDQIFCQnullDSKLEAADEGSGDVKYHLGMYHRWLNRVSNKYITMSLMANPSHLEAVDPVVQGKTKAEQFYSGDAEGKRVMSILLHGDAAFAGQGIVYETFHLSDLPSYTTHGTIHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNADDPEAVMYVCKVAAEWRNTFHKDVVVDLVCYRRNGHNEMDEPMFTQPLMYKKIKKQKGVLKIYVEKLIAEGVITLQEYQEEVAKYDKICEDAYSRSKDEKILHIKHWLDSPWPDFFTLDGQPKTMSCNSTGIPEEQLCHIGSIASSVPLKDFTIHGGLTRILKSRANMVSQRVCDWALGEYMAFGSLLQDGIHVRLSGQDVERGTFSHRHHVLHDQNVDKRICIPMNHISTSQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFHNTAQCIIDQFISPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDASPIVTEDFAVHQLFDCNWIVVNCSTPANYFHVLRRQILLPFRKPLIVFTPKSLLRHPEAKSSFDDMLPGTHFKRIIPDDGPTTVDPQKVKRVVFCTGKVYYELIRERKNRGMDDAVAVVRIEQLSPFPFDLVKAEIDQYINADLVWCQEEHKNQGYYDYVKPRISNTTQHRRPVWYVGREPAAAPATGNKHTHLVELQHFLDTAFDLEAFSGRQ

Target ORF information:

RefSeq Version	XM_011604775.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (ogdh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011604775.1

ORF Insert Sequence:

ATGCATCATT TAAGGACTAC CTTTCTGAGG CTTCGGCCTT TAACCGGTGC ACAGGCAGCT 
CAGAGATTGT CACAACCGCG CCTGTCGGTG ACCACAGCGG GCCCACGGAG GGTTCCGCCC 
TCCAGACAAT TGAACACGTC GGAGCCTTTT CTGAACGGCA CCAGCACCAA CTACATGGAG 
GAGATGTATT ATGCATGGCT GGAAAATCCA AAGAATGTCC ACAAGTCTTG GGATGTGTTT 
TTCCGTAACG TCAACGCCGG GGTTCCACCT GGGGCTGCTT ACCAGAGCCC CTCGTCCCTC 
CTCAAGCCTT CTCAGGGACT GACCGGTGCC CAGACACTTG TGGGGGCTCA GCCCAATATC 
GAGGAGCTGG TGGCGGATCA CTTAGCCGTG CAATCCCTCG TTCGAGCATA CCAGGTACGA 
GGGCACCACA TAGCAAAGTT AGACCCTCTG GAGATCAGCT GTGTGGACTT TGATGATGCA 
CCATGCGCCA TTGGCTTCCA GAACATTGGT TTTAATGGCC TGAACGAATC CCATTTGGAC 
AAAGTATTCC GGTTGCCAAA AACCACTTAC ATCGGAGGGG GTGAGAGTGC CCTGCCTCTC 
AGAGAGATTA TACGTCGGCT AGAGATGGCC TACTGTCAGC ACATTGGTGT GGAGTTCATG 
TTCATCAATG ACGTGCAGCA GTGCCATTGG ATCAGAGAAA AGTTTGAGAC ACCTGGAATC 
ATGAAGTTCA GTCTCGAGGA GAAGAGGACT CTTTTAGGCC GGGTGATCAG ATCCACAAGG 
TTTGAGGAGT TCCTCCAGAA GAAGTGGTCT TCAGAGAAAC GATTCGGCCT GGAAGGATGT 
GAATCCCTCA TCCCTGCCCT CAAGACAATC ATTGACATTT CCAGTCAGAG CGGAGTGGAG 
AGCATTATCA TGGGAATGCC TCACAGGGGA AGACTGAATG TGCTGGCCAA TGTCATTCGC 
AAGGAACTCG ACCAGATCTT CTGCCAGNTT GACTCCAAGC TTGAGGCTGC AGATGAGGGC 
TCTGGCGATG TGAAATATCA CTTGGGGATG TATCACAGGT GGTTGAACCG AGTCAGCAAC 
AAGTACATCA CAATGTCACT CATGGCGAAC CCCTCCCACC TGGAAGCCGT GGATCCGGTG 
GTGCAGGGCA AGACGAAAGC GGAGCAGTTC TACAGCGGAG ACGCAGAGGG CAAGAGGGTG 
ATGTCCATTC TGCTGCACGG TGATGCTGCG TTCGCAGGCC AGGGGATCGT TTACGAGACC 
TTCCACCTGT CTGACCTACC CTCCTACACC ACACACGGCA CAATACACGT GGTGGTCAAC 
AACCAGATCG GCTTCACCAC TGATCCCAGA ATGGCGCGCT CCTCCCCGTA CCCCACAGAT 
GTGGCTCGAG TCGTCAACGC TCCCATCTTC CACGTCAATG CCGATGACCC AGAGGCCGTG 
ATGTATGTGT GTAAGGTGGC AGCTGAATGG AGGAACACGT TCCATAAAGA TGTGGTTGTA 
GACCTGGTGT GCTACAGACG TAACGGCCAT AACGAGATGG ATGAGCCCAT GTTCACCCAG 
CCGCTGATGT ACAAGAAGAT CAAGAAGCAA AAGGGCGTCC TGAAAATTTA TGTAGAAAAG 
CTCATTGCTG AGGGAGTCAT CACACTACAA GAGTATCAGG AGGAAGTAGC AAAGTATGAC 
AAAATCTGCG AGGATGCGTA CAGTCGCTCC AAAGATGAAA AGATCCTTCA CATCAAACAC 
TGGCTGGACT CGCCGTGGCC CGATTTTTTT ACACTGGATG GACAACCAAA AACAATGAGC 
TGTAACTCCA CCGGCATCCC CGAAGAACAG CTCTGTCACA TCGGAAGCAT TGCTTCCTCG 
GTCCCACTAA AAGACTTCAC CATACACGGA GGTTTGACTC GTATCTTGAA GAGCCGTGCA 
AACATGGTGA GTCAGCGTGT GTGCGACTGG GCTCTGGGGG AATATATGGC CTTTGGCTCT 
CTGCTCCAGG ATGGAATCCA TGTACGTCTC AGTGGTCAAG ATGTGGAGAG GGGCACGTTT 
AGCCACCGGC ATCACGTTCT TCATGACCAG AACGTTGATA AGAGGATCTG CATCCCGATG 
AACCACATCT CCACCAGTCA AGCTCCTTAC ACCGTTTGCA ACAGCTCGCT GTCTGAGTAC 
GGAGTCTTAG GTTTTGAATT AGGCTTCGCT ATGGCCAGTC CAAATGCACT GGTTCTGTGG 
GAGGCCCAGT TTGGAGATTT TCACAACACA GCTCAGTGCA TCATCGACCA GTTCATCAGT 
CCGGGTCAGG CCAAATGGGT TCGACAGAAT GGCATCGTCC TGCTGCTTCC TCACGGCATG 
GAGGGAATGG GTCCAGAACA CTCATCTGCC CGGCCTGAAA GATTTCTACA GATGTGCAAC 
GATGACCCAG ATGCATCTCC TATTGTGACC GAAGACTTCG CAGTGCATCA GTTGTTCGAC 
TGTAACTGGA TTGTTGTCAA CTGCTCCACG CCCGCGAACT ACTTCCATGT TCTCCGCAGA 
CAGATCCTTC TGCCCTTCAG GAAGCCCCTG ATCGTGTTTA CTCCCAAGTC ACTGTTGCGC 
CACCCAGAGG CCAAATCCAG CTTTGATGAC ATGCTGCCAG GCACACACTT TAAGCGGATA 
ATCCCAGATG ACGGGCCCAC AACTGTCGAC CCACAAAAGG TGAAGAGAGT GGTTTTCTGC 
ACCGGCAAGG TCTACTATGA GCTGATCCGA GAACGCAAGA ACAGAGGCAT GGACGATGCC 
GTCGCGGTCG TGCGCATCGA GCAGCTCTCT CCATTCCCCT TTGATCTGGT GAAGGCTGAA 
ATCGATCAGT ACATCAATGC CGACCTGGTG TGGTGCCAGG AGGAGCACAA GAACCAGGGT 
TACTACGACT ACGTCAAGCC CCGCATCTCC AACACCACCC AGCACCGCCG CCCCGTCTGG 
TACGTTGGTC GTGAGCCTGC AGCGGCTCCC GCTACTGGAA ACAAACACAC CCATCTGGTG 
GAGCTCCAGC ATTTCCTGGA CACGGCCTTT GACCTGGAGG CATTCTCAGG AAGACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ogdh ( XM_029837362.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029693222.1 Takifugu rubripes 2-oxoglutarate dehydrogenase, mitochondrial (LOC101068525), mRNA.

Price & Availability↑

CloneID	OTo67738
Clone ID Related Accession (Same CDS sequence)	XM_029837362.1
Accession Version	XM_029837362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	2-oxoglutarate dehydrogenase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042290.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCATT TAAGGACTAC CTTTCTGAGG CTTCGGCCTT TAACCGGTGC ACAGGCAGCT 
CAGAGATTGT CACAACCGCG CCTGTCGGTG ACCACAGCGG GCCCACGGAG GGTTCCGCCC 
TCCAGACAAT TGAACACGTC GGAGCCTTTT CTGAACGGCA CCAGCACCAA CTACATGGAG 
GAGATGTATT ATGCATGGCT GGAAAATCCA AAGAATGTCC ACAAGTCTTG GGATGTGTTT 
TTCCGTAACG TCAACGCCGG GGTTCCACCT GGGGCTGCTT ACCAGAGCCC CTCGTCCCTC 
CTCAAGCCTT CTCAGGGACT GACCGGTGCC CAGACACTTG TGGGGGCTCA GCCCAATATC 
GAGGAGCTGG TGGCGGATCA CTTAGCCGTG CAATCCCTCG TTCGAGCATA CCAGGTACGA 
GGGCACCACA TAGCAAAGTT AGACCCTCTG GAGATCAGCT GTGTGGACTT TGATGATGCA 
CCATGCGCCA TTGGCTTCCA GAACATTGGT TTTAATGGCC TGAACGAATC CCATTTGGAC 
AAAGTATTCC GGTTGCCAAA AACCACTTAC ATCGGAGGGG GTGAGAGTGC CCTGCCTCTC 
AGAGAGATTA TACGTCGGCT AGAGATGGCC TACTGTCAGC ACATTGGTGT GGAGTTCATG 
TTCATCAATG ACGTGCAGCA GTGCCATTGG ATCAGAGAAA AGTTTGAGAC ACCTGGAATC 
ATGAAGTTCA GTCTCGAGGA GAAGAGGACT CTTTTAGGCC GGGTGATCAG ATCCACAAGG 
TTTGAGGAGT TCCTCCAGAA GAAGTGGTCT TCAGAGAAAC GATTCGGCCT GGAAGGATGT 
GAATCCCTCA TCCCTGCCCT CAAGACAATC ATTGACATTT CCAGTCAGAG CGGAGTGGAG 
AGCATTATCA TGGGAATGCC TCACAGGGGA AGACTGAATG TGCTGGCCAA TGTCATTCGC 
AAGGAACTCG ACCAGATCTT CTGCCAGTTT GACTCCAAGC TTGAGGCTGC AGATGAGGGC 
TCTGGCGATG TGAAATATCA CTTGGGGATG TATCACAGGT GGTTGAACCG AGTCAGCAAC 
AAGTACATCA CAATGTCACT CATGGCGAAC CCCTCCCACC TGGAAGCCGT GGATCCGGTG 
GTGCAGGGCA AGACGAAAGC GGAGCAGTTC TACAGCGGAG ACGCAGAGGG CAAGAGGGTG 
ATGTCCATTC TGCTGCACGG TGATGCTGCG TTCGCAGGCC AGGGGATCGT TTACGAGACC 
TTCCACCTGT CTGACCTACC CTCCTACACC ACACACGGCA CAATACACGT GGTGGTCAAC 
AACCAGATCG GCTTCACCAC TGATCCCAGA ATGGCGCGCT CCTCCCCGTA CCCCACAGAT 
GTGGCTCGAG TCGTCAACGC TCCCATCTTC CACGTCAATG CCGATGACCC AGAGGCCGTG 
ATGTATGTGT GTAAGGTGGC AGCTGAATGG AGGAACACGT TCCATAAAGA TGTGGTTGTA 
GACCTGGTGT GCTACAGACG TAACGGCCAT AACGAGATGG ATGAGCCCAT GTTCACCCAG 
CCGCTGATGT ACAAGAAGAT CAAGAAGCAA AAGGGCGTCC TGAAAATTTA TGTAGAAAAG 
CTCATTGCTG AGGGAGTCAT CACACTACAA GAGTATCAGG AGGAAGTAGC AAAGTATGAC 
AAAATCTGCG AGGATGCGTA CAGTCGCTCC AAAGATGAAA AGATCCTTCA CATCAAACAC 
TGGCTGGACT CGCCGTGGCC CGATTTTTTT ACACTGGATG GACAACCAAA AACAATGAGC 
TGTAACTCCA CCGGCATCCC CGAAGAACAG CTCTGTCACA TCGGAAGCAT TGCTTCCTCG 
GTCCCACTAA AAGACTTCAC CATACACGGA GGTTTGACTC GTATCTTGAA GAGCCGTGCA 
AACATGGTGA GTCAGCGTGT GTGCGACTGG GCTCTGGGGG AATATATGGC CTTTGGCTCT 
CTGCTCCAGG ATGGAATCCA TGTACGTCTC AGTGGTCAAG ATGTGGAGAG GGGCACGTTT 
AGCCACCGGC ATCATGTTCT TCATGACCAG AACGTTGATA AGAGGATCTG CATCCCGATG 
AACCACATCT CCACCAGTCA AGCTCCTTAC ACCGTTTGCA ACAGCTCGCT GTCTGAGTAC 
GGAGTCTTAG GTTTTGAATT AGGCTTCGCT ATGGCCAGTC CAAATGCACT GGTTCTGTGG 
GAGGCCCAGT TTGGAGATTT TCACAACACA GCTCAGTGCA TCATCGACCA GTTCATCAGT 
CCGGGTCAGG CCAAATGGGT TCGACAGAAT GGCATCGTCC TGCTGCTTCC TCACGGCATG 
GAGGGAATGG GTCCAGAACA CTCATCTGCC CGGCCTGAAA GATTTCTACA GATGTGCAAC 
GATGACCCAG ATGCATCTCC TATTGTGACC GAAGACTTCG CAGTGCATCA GTTGTTCGAC 
TGTAACTGGA TTGTTGTCAA CTGCTCCACG CCCGCGAACT ACTTCCATGT TCTCCGCAGA 
CAGATCCTTC TGCCCTTCAG GAAGCCCCTG ATCGTGTTTA CTCCCAAGTC ACTGTTGCGC 
CACCCAGAGG CCAAATCCAG CTTTGATGAC ATGCTGCCAG GCACACACTT TAAGCGGATA 
ATCCCAGATG ACGGGCCCAC AACTGTCGAC CCACAAAAGG TGAAGAGAGT GGTTTTCTGC 
ACCGGCAAGG TCTACTATGA GCTGATCCGA GAACGCAAGA ACAGAGGCAT GGACGATGCC 
GTCGCGGTCG TGCGCATCGA GCAGCTCTCT CCATTCCCCT TTGATCTGGT GAAGGCTGAA 
ATCGATCAGT ACATCAATGC CGACCTGGTG TGGTGCCAGG AGGAGCACAA GAACCAGGGT 
TACTACGACT ACGTCAAGCC CCGCATCTCC AACACCACCC AGCACCGCCG CCCCGTCTGG 
TACGTTGGTC GTGAGCCTGC AGCGGCTCCC GCTACTGGAA ACAAACACAC CCATCTGGTG 
GAGCTCCAGC ATTTCCTGGA CACGGCCTTT GACCTGGAGG CATTCTCAGG AAGACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029693222.1
CDS	400..3459
Translation	MHHLRTTFLRLRPLTGAQAAQRLSQPRLSVTTAGPRRVPPSRQLNTSEPFLNGTSTNYMEEMYYAWLENPKNVHKSWDVFFRNVNAGVPPGAAYQSPSSLLKPSQGLTGAQTLVGAQPNIEELVADHLAVQSLVRAYQVRGHHIAKLDPLEISCVDFDDAPCAIGFQNIGFNGLNESHLDKVFRLPKTTYIGGGESALPLREIIRRLEMAYCQHIGVEFMFINDVQQCHWIREKFETPGIMKFSLEEKRTLLGRVIRSTRFEEFLQKKWSSEKRFGLEGCESLIPALKTIIDISSQSGVESIIMGMPHRGRLNVLANVIRKELDQIFCQFDSKLEAADEGSGDVKYHLGMYHRWLNRVSNKYITMSLMANPSHLEAVDPVVQGKTKAEQFYSGDAEGKRVMSILLHGDAAFAGQGIVYETFHLSDLPSYTTHGTIHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNADDPEAVMYVCKVAAEWRNTFHKDVVVDLVCYRRNGHNEMDEPMFTQPLMYKKIKKQKGVLKIYVEKLIAEGVITLQEYQEEVAKYDKICEDAYSRSKDEKILHIKHWLDSPWPDFFTLDGQPKTMSCNSTGIPEEQLCHIGSIASSVPLKDFTIHGGLTRILKSRANMVSQRVCDWALGEYMAFGSLLQDGIHVRLSGQDVERGTFSHRHHVLHDQNVDKRICIPMNHISTSQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFHNTAQCIIDQFISPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDASPIVTEDFAVHQLFDCNWIVVNCSTPANYFHVLRRQILLPFRKPLIVFTPKSLLRHPEAKSSFDDMLPGTHFKRIIPDDGPTTVDPQKVKRVVFCTGKVYYELIRERKNRGMDDAVAVVRIEQLSPFPFDLVKAEIDQYINADLVWCQEEHKNQGYYDYVKPRISNTTQHRRPVWYVGREPAAAPATGNKHTHLVELQHFLDTAFDLEAFSGRQ

Target ORF information:

RefSeq Version	XM_029837362.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes 2-oxoglutarate dehydrogenase, mitochondrial (LOC101068525), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029837362.1

ORF Insert Sequence:

ATGCATCATT TAAGGACTAC CTTTCTGAGG CTTCGGCCTT TAACCGGTGC ACAGGCAGCT 
CAGAGATTGT CACAACCGCG CCTGTCGGTG ACCACAGCGG GCCCACGGAG GGTTCCGCCC 
TCCAGACAAT TGAACACGTC GGAGCCTTTT CTGAACGGCA CCAGCACCAA CTACATGGAG 
GAGATGTATT ATGCATGGCT GGAAAATCCA AAGAATGTCC ACAAGTCTTG GGATGTGTTT 
TTCCGTAACG TCAACGCCGG GGTTCCACCT GGGGCTGCTT ACCAGAGCCC CTCGTCCCTC 
CTCAAGCCTT CTCAGGGACT GACCGGTGCC CAGACACTTG TGGGGGCTCA GCCCAATATC 
GAGGAGCTGG TGGCGGATCA CTTAGCCGTG CAATCCCTCG TTCGAGCATA CCAGGTACGA 
GGGCACCACA TAGCAAAGTT AGACCCTCTG GAGATCAGCT GTGTGGACTT TGATGATGCA 
CCATGCGCCA TTGGCTTCCA GAACATTGGT TTTAATGGCC TGAACGAATC CCATTTGGAC 
AAAGTATTCC GGTTGCCAAA AACCACTTAC ATCGGAGGGG GTGAGAGTGC CCTGCCTCTC 
AGAGAGATTA TACGTCGGCT AGAGATGGCC TACTGTCAGC ACATTGGTGT GGAGTTCATG 
TTCATCAATG ACGTGCAGCA GTGCCATTGG ATCAGAGAAA AGTTTGAGAC ACCTGGAATC 
ATGAAGTTCA GTCTCGAGGA GAAGAGGACT CTTTTAGGCC GGGTGATCAG ATCCACAAGG 
TTTGAGGAGT TCCTCCAGAA GAAGTGGTCT TCAGAGAAAC GATTCGGCCT GGAAGGATGT 
GAATCCCTCA TCCCTGCCCT CAAGACAATC ATTGACATTT CCAGTCAGAG CGGAGTGGAG 
AGCATTATCA TGGGAATGCC TCACAGGGGA AGACTGAATG TGCTGGCCAA TGTCATTCGC 
AAGGAACTCG ACCAGATCTT CTGCCAGTTT GACTCCAAGC TTGAGGCTGC AGATGAGGGC 
TCTGGCGATG TGAAATATCA CTTGGGGATG TATCACAGGT GGTTGAACCG AGTCAGCAAC 
AAGTACATCA CAATGTCACT CATGGCGAAC CCCTCCCACC TGGAAGCCGT GGATCCGGTG 
GTGCAGGGCA AGACGAAAGC GGAGCAGTTC TACAGCGGAG ACGCAGAGGG CAAGAGGGTG 
ATGTCCATTC TGCTGCACGG TGATGCTGCG TTCGCAGGCC AGGGGATCGT TTACGAGACC 
TTCCACCTGT CTGACCTACC CTCCTACACC ACACACGGCA CAATACACGT GGTGGTCAAC 
AACCAGATCG GCTTCACCAC TGATCCCAGA ATGGCGCGCT CCTCCCCGTA CCCCACAGAT 
GTGGCTCGAG TCGTCAACGC TCCCATCTTC CACGTCAATG CCGATGACCC AGAGGCCGTG 
ATGTATGTGT GTAAGGTGGC AGCTGAATGG AGGAACACGT TCCATAAAGA TGTGGTTGTA 
GACCTGGTGT GCTACAGACG TAACGGCCAT AACGAGATGG ATGAGCCCAT GTTCACCCAG 
CCGCTGATGT ACAAGAAGAT CAAGAAGCAA AAGGGCGTCC TGAAAATTTA TGTAGAAAAG 
CTCATTGCTG AGGGAGTCAT CACACTACAA GAGTATCAGG AGGAAGTAGC AAAGTATGAC 
AAAATCTGCG AGGATGCGTA CAGTCGCTCC AAAGATGAAA AGATCCTTCA CATCAAACAC 
TGGCTGGACT CGCCGTGGCC CGATTTTTTT ACACTGGATG GACAACCAAA AACAATGAGC 
TGTAACTCCA CCGGCATCCC CGAAGAACAG CTCTGTCACA TCGGAAGCAT TGCTTCCTCG 
GTCCCACTAA AAGACTTCAC CATACACGGA GGTTTGACTC GTATCTTGAA GAGCCGTGCA 
AACATGGTGA GTCAGCGTGT GTGCGACTGG GCTCTGGGGG AATATATGGC CTTTGGCTCT 
CTGCTCCAGG ATGGAATCCA TGTACGTCTC AGTGGTCAAG ATGTGGAGAG GGGCACGTTT 
AGCCACCGGC ATCATGTTCT TCATGACCAG AACGTTGATA AGAGGATCTG CATCCCGATG 
AACCACATCT CCACCAGTCA AGCTCCTTAC ACCGTTTGCA ACAGCTCGCT GTCTGAGTAC 
GGAGTCTTAG GTTTTGAATT AGGCTTCGCT ATGGCCAGTC CAAATGCACT GGTTCTGTGG 
GAGGCCCAGT TTGGAGATTT TCACAACACA GCTCAGTGCA TCATCGACCA GTTCATCAGT 
CCGGGTCAGG CCAAATGGGT TCGACAGAAT GGCATCGTCC TGCTGCTTCC TCACGGCATG 
GAGGGAATGG GTCCAGAACA CTCATCTGCC CGGCCTGAAA GATTTCTACA GATGTGCAAC 
GATGACCCAG ATGCATCTCC TATTGTGACC GAAGACTTCG CAGTGCATCA GTTGTTCGAC 
TGTAACTGGA TTGTTGTCAA CTGCTCCACG CCCGCGAACT ACTTCCATGT TCTCCGCAGA 
CAGATCCTTC TGCCCTTCAG GAAGCCCCTG ATCGTGTTTA CTCCCAAGTC ACTGTTGCGC 
CACCCAGAGG CCAAATCCAG CTTTGATGAC ATGCTGCCAG GCACACACTT TAAGCGGATA 
ATCCCAGATG ACGGGCCCAC AACTGTCGAC CCACAAAAGG TGAAGAGAGT GGTTTTCTGC 
ACCGGCAAGG TCTACTATGA GCTGATCCGA GAACGCAAGA ACAGAGGCAT GGACGATGCC 
GTCGCGGTCG TGCGCATCGA GCAGCTCTCT CCATTCCCCT TTGATCTGGT GAAGGCTGAA 
ATCGATCAGT ACATCAATGC CGACCTGGTG TGGTGCCAGG AGGAGCACAA GAACCAGGGT 
TACTACGACT ACGTCAAGCC CCGCATCTCC AACACCACCC AGCACCGCCG CCCCGTCTGG 
TACGTTGGTC GTGAGCCTGC AGCGGCTCCC GCTACTGGAA ACAAACACAC CCATCTGGTG 
GAGCTCCAGC ATTTCCTGGA CACGGCCTTT GACCTGGAGG CATTCTCAGG AAGACAGTAG