plce1 gene cDNA ORF clone, Takifugu rubripes(torafugu)

mRNA	Protein	Name
XM_011606171.1	XP_011604473.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
XM_029841815.1	XP_029697675.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X6
XM_011606185.2	XP_011604487.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X7
XM_029841545.1	XP_029697405.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
XM_011606177.1	XP_011604479.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
XM_011606185.1	XP_011604487.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X4
XM_029841511.1	XP_029697371.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
XM_029841585.1	XP_029697445.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
XM_029841640.1	XP_029697500.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
XM_011606166.2	XP_011604468.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X4
XM_011606166.1	XP_011604468.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
XM_029841478.1	XP_029697338.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
XM_029841739.1	XP_029697599.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo22206	XM_011606171.1 Latest version!	Takifugu rubripes phospholipase C, epsilon 1 (plce1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OTo22207	XM_011606177.1 Latest version!	Takifugu rubripes phospholipase C, epsilon 1 (plce1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OTo22208	XM_011606185.1 Latest version!	Takifugu rubripes phospholipase C, epsilon 1 (plce1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OTo40717	XM_029841545.1 Latest version!	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OTo40717	XM_029841478.1 Latest version!	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OTo40717	XM_029841511.1 Latest version!	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OTo40718	XM_029841585.1 Latest version!	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OTo40719	XM_029841640.1 Latest version!	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OTo40720	XM_011606166.2 Latest version!	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OTo40721	XM_029841739.1 Latest version!	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OTo40722	XM_029841815.1 Latest version!	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40723	XM_011606185.2 Latest version!	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo22205	XM_011606166.1 Latest version!	Takifugu rubripes phospholipase C, epsilon 1 (plce1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

plce1 ( XM_011606171.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011604473.1 Takifugu rubripes phospholipase C, epsilon 1 (plce1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo22206
Clone ID Related Accession (Same CDS sequence)	XM_011606171.1
Accession Version	XM_011606171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070902.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCACT CGCACAAAGT GGACTTCAAG CTGATCAAGC GTCAGCTGGC CAACAGCATG 
ACCAGCAGCA GTGCGCTGTC TCCCGCCGTC AGCGGCATCA GTAAGGAGCT GGCAGAAATG 
CGGCACCTTG TTCAGTTCCC CGAGGAGATC GCCTGCATCC TCACTGAGCA GGAGCAGCAG 
CTCTACCAGC GGGTTTTCCC CCTGGACTAC CTCTGTTTCC TTACAAGAGA CCTGGGCAGT 
CCCGAGTGTC AAAGCAAACA TCACCCAAGC CTTAAGGCGT CACTTTCTGT GCCAGCCATG 
TCTACACAGA GCTCTCGACA CAATGCAGTG GAGGACCTGG TGGCACGCTT CAATGAGGTG 
AGCTCATGGG TAACCTGGCT GATTCTGACA GCAGGGTCCA TGGAAGAGAA GAGAGAGGTC 
TTCTCCTATC TGGTCAATGT TGCCAAGTGC TGTTGGAACA TGGGCAATTA CAATGGCGTC 
ATGGAGTTCC TGGCTGGACT CAGGTCCCGT AAGGTGCTGA AAATGTGGCA ATTTATGGAC 
CAGACTGACA TCGAGACCAT GCGTAGCCTG AAGGATGCTA TGGCTCAACA TGAATCCTCC 
TCTGAGTACA AGAAGGTTGT GACCAGAGCT CTCAACATCC CAGGATGTAA GGTGGTGCCT 
TTCTGTGGGG TCTTTCTAAA GGAGCTGAGT GATGCACTGG ATGGAACAGC AAGCATAATC 
AGCCTGAAAT CTCCTCTGGA GAATTCAGAA GACTCAATAG AGTTTGTTTC AGACTATAGC 
GGCCAGCACA ACTTCCTGTT GCGGTCTGGT CCTGATGGTC TACACATCCC AGAGAAAGAA 
GCTACTGTCA GTAACATTCT CCAGATTATC AGATCCTGTA ATCGTAGTTT GGAGGTGGAA 
GACACTGATG ATGGCTCAAC CAGCCCTTCT TCTAGTTGTC CACTCCCCAC TTCCAGAAAC 
AACTCCTTCA AGGACCGCTG CCGTAATCAT TCACGTTCAT TTAAGATGCC CCAAGAAACA 
AAACGTGCCT TGTTCATGGT GGGAGACTTG TCAGATTCAG ACGGAGACCT GCCATCTGAG 
CCTGCAGTGA AAGAGGGGGA GTTTCAGGGG ACTGAAGAAA CACACAAGGC TTTCAACCAT 
GGCACCGAGC TCATTCCCTG GTACGTGTTA TCGCTGCAGC CGGATGTTCA CCAGTTTCTG 
CTGCAGGGGG CCACGGTCAT CCACTACGAC CAGGACAGCC ACCTCTCAGC ACGCTGTCTG 
CTGCGGCTGC AGCCCGACAA CACAACGCTC ACCTGGGGTA ACCCACAGAA AGGTGGGGCC 
TCCCCCACAG AGCCACCACT GGGATTAGGA CAGGCCGTCG TCGCAGGACT AGCCGAGGGG 
CTTTTGGACC TTGGAGTGGT GAAGGCAGTG TTCCTAGGTC ATCAGGGAGT CGATGTTCAT 
GCTGTATGTC TGCAAAACAA GCTGAGCCAC ATGACAGTGG AGGAGAACAC CCTGAGCCTC 
CTCTATGGTG TCAGTACCAC TGACAACAGA CTTCTGCACT TTGTGGCTCC AAACCACACC 
ACGCAAATGC TCCATAAAGG TTTGTCAGAG TTGGTGACGG CCACACGGAA ATTGAAGAAG 
TTTCCTGATC AGCGGTTGCA GTGGCTGCGG AGGCAGTATG TCAGTTTATA TCAGGAGGAT 
GGCAGGTATG AAGGGCCCAC ATTAGCCCAA GCCATTGAGC TGTTTGGAGG CCGGAGATGG 
AACATGAGCA CAGGTGGAAC AGAGAAATCT GCCCATCAGA AAAACAGTCC TTTGAGCATC 
AACGATAAGA CCAAGAAGAA GAAGAAGGTC CTCGTCAGGG GAGACAGTGG AGATGCCACT 
GACGATGAGA TGGTTGCTCG GAAAACACGG AGCTGTAAGG AAGGAACATA TCGAAACGGA 
CCGGAGTCTG ACAGTATAGA TCACGAAGAC CCAGAAGATA GCTTCCCGGG ACCATTCTCC 
CTCTCAACCA ATAAAAGTCC TGGATTGCAA GCCTCTTCTT CTTCCTCCTC CTCCTCTTCC 
AGCATGACTG GCTCCAATCA GTCTCGCCCA CAGTCCTCCC CAACCCTCTC AGGAACAGTG 
AAAGCACAGC CAGGGGCGTG GAGCAGCAGG TCATGGCATG GTCGTGGTAA AGGCTGCTTC 
AGAGGCTTCC AGAACCTCAT GATCTCTGAC AGCACAATGA GCTTTGTTGA GTTTGTGGAG 
CTTTTCAAGT CATTCAGTAT CCGAAGCAGG AAGGATCTGA AGGAACTGTT TGACACCTTT 
GCTGTGCCCT GCATTCGCTC AGATCCAGAG TCTGTTCCTC TTTACACCAA TCTCAGGATA 
GATGACAAAG ACACAGGTCT ACAGCCGGAC CTCGATTTAT TAACGCGCAA TGGTTCGGAT 
CTTGGTCTGT TTATCCGAAC GAGGCAGCAG ATGTCTGACA ACCAGAAGCA GATCTCAGAC 
GCCATTGCAG CAGCCAGCAT TGTGACCAAC GGGACGGGAG TAGAAAACGC ATCACTGGGG 
GTTTTGGGAC TGGGCATCCC TCAACTTAAC GACTTCTTGG TCAACTGTCA GAGAGAACAT 
CTGAGCTATG ATGAGATCCT CAGCATTATA CAGAAGTTTG AACCCTCATC AAGCATGAGA 
CAAATGGGGT GGATGTCATT CGAAGGCTTT TCAAGGTTCC TGATGGATAA GGATAACTTT 
GCCTCACACA TTGAGGAATC CCAGATGAAC CCGGAGGAAC TGCAGCATCC CCTGTCTTAT 
TACTACATTC AGTCTTCTCA CAATACTTAT CTGACTGGGC ACCAGCTTAA AGGGGAGTCC 
TCTGTTGAGC TTTACAGTCA GGTGCTTTTG CAAGGCTGCA GAAGTGTGGA GCTGGACTGT 
TGGGATGGAG ATGATGGCAT GCCGGTTATT TATCACGGAC ACACTCTTAC CACCAAGATA 
CCATTTAAGG ATGTGGTGGA AGCAATTAGC AGGTCTGCAT TTGTCAACTC CAACATGCCC 
GTCGTCCTGT CTATAGAGAA CCACTGCTCC CTCCCGCAGC AGCGCAAGAT GGCGGAAATC 
TTCAAGACGG TGTTTGGGGA GCGTCTGGTG ACACGCTTCC TCTTTGAAAG CGACTTCAGC 
GATGACCCTC ACCTGCCATC TCCACTGCAG CTGCAGGGGA GGATTCTCCT CAAGAATAAG 
AAGCTGAAAG CACACCAGGC TCCGGTGGAC ATACTCAAGC AGAAGGCTCA CCAGCTTGCC 
CACATGCAGG CCCAGGTTAG CAATGGTTCA CCAGGGGTTT CCTCTCCCGG GACCCAAACC 
AACGAGGACG AGGAAGAGGA AGAAGACGAG TACGACTATG ACTATGAGTC TCTCTCAGAT 
GACAACCTCC TGGATGACAA ACCTGAGGTG AAAAGCTCAG CTGACAAAGA GGAGCAGCCT 
GTGGATGAAA TACCCAAGAG AATGAAGAAG CCTGACAACA CTACGCAGAG TAAAGGAAAG 
GTGTTTGACA TGGAACTGGG CGAGGAGTTC TACCTTCCAC AGAACAAAAA GGAAAGTCGA 
CAGATCGCTC AGGAGCTGTC TGACCTCGTC ATCTACTGTC AGGCCGTGAA GTTCCCTGGT 
CTGTCCACGC TGTCTCCGGC TGGCTCCGGC AGAGGGAAAG ACCGTGGAAA GAGCAGGAAG 
TCCATATTTG GCACGGCTCC AGCTCGCTGC AGCGCAACAG GGGAGGTCAC CACTCCGAGT 
CGCACCCCTG GGAAAGGTGG CGCCGAGCGC CTGAGCTGGG AAGAGCAGCA AACGTCTCCT 
GTCCTCAGTC CACCCACCTC TCTCAGCGCC ATTATCCGTA CACCCAAGTG TTATCACATC 
TCCTCCGTCA ATGAGAACGC CGCCAAGCGA CTGTGCCGGC GTTACTCTCA GAAACTGATC 
CAGCACACCG TGTGTCAGCT GCTCAGGACT TACCCAGCAG CCACCAGGAT CGACTCCACA 
AACCCAAACC CTCTGCTGTT CTGGCTGCAT GGCATTCAGC TGGTGGCTCT CAACTATCAG 
ACTGACGACC TGCCCATGCA GTTAAACACA GCTCTGTTTG AGGCCAACGG CGGCTGCGGC 
TATGTCCTGA AACCAGCGGT GCTGTGGGAC CGAAACTGTC CGATGTATCA GCAGTTCTGT 
CCGATGGAGA GGGATGTGGA AAAAATGAGT CCTGCCGTCT ACTCCCTTGC TATCGTTTCG 
GGGCAGAACG TCTGTCCCGG TAACAGCAGC GGCAGCCCCT GCATCGAGGT GGACGTCCTG 
GGGATGCCGG TCGACAGCGC CCACTTCCGT ACCAAACCCA TCCACCGCAA CACCCTCAAC 
CCCATGTGGA ACGAGCACTT TCAGTTCACC GTGCACTTTG AAGAGATGTG CTTCTTGCGC 
GTTGCTGTTG TGGAGAACAA CAGTTCCCAG ACTACTGCTC AGAGAACTCT ACCTCTGAAA 
GCCCTCAAGT CAGGTTACAG ACACATCCAG CTGAGGACTC AACACAATGA ATCTCTGGAA 
GTGTCAAGCT TGTTCATCTA TAGCCGAAGG ACAGAGGAGT GTCCGACAGG GGGCGACATT 
CCCTCATCCC TGCTGTTCAG CTCTGAGGAG AAGCCTGCAT CCCAGCAGCA CAGGGTGACG 
GTGTACGGAG CTCCTGGTCC TGAGCCCTTC ACTGTGTTCA GTGTGACGGA GCAGATCACT 
GCCAAACAGC TGCTGGACAT GGTCGTAGCT TCTGCTGGTG GAAATCCCTA CTTCCTGTGT 
GAGGAGAAGG TTCCTTTGAC GAAGGAGCGC AGTGAGACAA AACGCTGTGC TCAGTATCGC 
CCCCTTGCTC CGGAGGAGGA GGTGGTCCGA CTGGTTTCCA GCTGGAGTGC CGAGGAGGGA 
TATGTGGGAA GGATCTGCTT CAAGCTCAGA GAGGAGAAAT TAAATGAGAA GAACGCAGCA 
CCAGAAGGAG AGGAGGAGTG GAGTGTGGGC GGAGCCAGGG AGGGAGCTGG TGGAGAAGGA 
GCAGCAGCAG CAGAGGATGA TGTGTTCCTG GTCCAGATCC ATGAAGTTTC TCCAGAGCAG 
CCTCACACTG TGATCAAGGC CCCGCGCTAC AGCACAGCTC AGGACATCAT CCAGCAGACG 
TTATTCAAGG CCAAGTATTC CTACAGCATC CTCTCCAATC CCAATCCATG CGACTACGTC 
CTAATGGAGG AAGTAACCAA AGACGTGGGC TCAAAGAAGT CCTCCACCAC TAAGCCCCTG 
CAGCGGATGC TGCTGGATCA CGAGTGCGTC TATCAGGCTC AGAGCCGCTG GCGGGGCGCG 
GGGAAGTTCA TTCTGAAACT GAAAGAGCAG TTGGTGCGGG AAGACAAAAA GAAAGTCATT 
TCCTTTGCCA GTGAGCTGAA GAAACTGACG AGCCGCGGCC GCAGCATGAC GACTGGCGGC 
GGCGTGGACG GCCCCGGCCA GAGGTGTTGG TCTGCAGCAG TCGGTCTGAA GGTGTGGGTG 
GCAGATGGAA GGGTGGGGGC AGGTAGATTC CTTCCATCTT CCTCCTCTGG GAGACGTCTT 
CATCCAATCC ACCTGCCCAC CTCCTCTCTG ACTGCACCAG TGTTGAAGCT GCACCTCCTC 
AGGAACTGGA AGATCCACAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604473.1
CDS	1292..6895
Translation	MKHSHKVDFKLIKRQLANSMTSSSALSPAVSGISKELAEMRHLVQFPEEIACILTEQEQQLYQRVFPLDYLCFLTRDLGSPECQSKHHPSLKASLSVPAMSTQSSRHNAVEDLVARFNEVSSWVTWLILTAGSMEEKREVFSYLVNVAKCCWNMGNYNGVMEFLAGLRSRKVLKMWQFMDQTDIETMRSLKDAMAQHESSSEYKKVVTRALNIPGCKVVPFCGVFLKELSDALDGTASIISLKSPLENSEDSIEFVSDYSGQHNFLLRSGPDGLHIPEKEATVSNILQIIRSCNRSLEVEDTDDGSTSPSSSCPLPTSRNNSFKDRCRNHSRSFKMPQETKRALFMVGDLSDSDGDLPSEPAVKEGEFQGTEETHKAFNHGTELIPWYVLSLQPDVHQFLLQGATVIHYDQDSHLSARCLLRLQPDNTTLTWGNPQKGGASPTEPPLGLGQAVVAGLAEGLLDLGVVKAVFLGHQGVDVHAVCLQNKLSHMTVEENTLSLLYGVSTTDNRLLHFVAPNHTTQMLHKGLSELVTATRKLKKFPDQRLQWLRRQYVSLYQEDGRYEGPTLAQAIELFGGRRWNMSTGGTEKSAHQKNSPLSINDKTKKKKKVLVRGDSGDATDDEMVARKTRSCKEGTYRNGPESDSIDHEDPEDSFPGPFSLSTNKSPGLQASSSSSSSSSSMTGSNQSRPQSSPTLSGTVKAQPGAWSSRSWHGRGKGCFRGFQNLMISDSTMSFVEFVELFKSFSIRSRKDLKELFDTFAVPCIRSDPESVPLYTNLRIDDKDTGLQPDLDLLTRNGSDLGLFIRTRQQMSDNQKQISDAIAAASIVTNGTGVENASLGVLGLGIPQLNDFLVNCQREHLSYDEILSIIQKFEPSSSMRQMGWMSFEGFSRFLMDKDNFASHIEESQMNPEELQHPLSYYYIQSSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPVIYHGHTLTTKIPFKDVVEAISRSAFVNSNMPVVLSIENHCSLPQQRKMAEIFKTVFGERLVTRFLFESDFSDDPHLPSPLQLQGRILLKNKKLKAHQAPVDILKQKAHQLAHMQAQVSNGSPGVSSPGTQTNEDEEEEEDEYDYDYESLSDDNLLDDKPEVKSSADKEEQPVDEIPKRMKKPDNTTQSKGKVFDMELGEEFYLPQNKKESRQIAQELSDLVIYCQAVKFPGLSTLSPAGSGRGKDRGKSRKSIFGTAPARCSATGEVTTPSRTPGKGGAERLSWEEQQTSPVLSPPTSLSAIIRTPKCYHISSVNENAAKRLCRRYSQKLIQHTVCQLLRTYPAATRIDSTNPNPLLFWLHGIQLVALNYQTDDLPMQLNTALFEANGGCGYVLKPAVLWDRNCPMYQQFCPMERDVEKMSPAVYSLAIVSGQNVCPGNSSGSPCIEVDVLGMPVDSAHFRTKPIHRNTLNPMWNEHFQFTVHFEEMCFLRVAVVENNSSQTTAQRTLPLKALKSGYRHIQLRTQHNESLEVSSLFIYSRRTEECPTGGDIPSSLLFSSEEKPASQQHRVTVYGAPGPEPFTVFSVTEQITAKQLLDMVVASAGGNPYFLCEEKVPLTKERSETKRCAQYRPLAPEEEVVRLVSSWSAEEGYVGRICFKLREEKLNEKNAAPEGEEEWSVGGAREGAGGEGAAAAEDDVFLVQIHEVSPEQPHTVIKAPRYSTAQDIIQQTLFKAKYSYSILSNPNPCDYVLMEEVTKDVGSKKSSTTKPLQRMLLDHECVYQAQSRWRGAGKFILKLKEQLVREDKKKVISFASELKKLTSRGRSMTTGGGVDGPGQRCWSAAVGLKVWVADGRVGAGRFLPSSSSGRRLHPIHLPTSSLTAPVLKLHLLRNWKIHK

Target ORF information:

RefSeq Version	XM_011606171.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phospholipase C, epsilon 1 (plce1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606171.1

ORF Insert Sequence:

ATGAAGCACT CGCACAAAGT GGACTTCAAG CTGATCAAGC GTCAGCTGGC CAACAGCATG 
ACCAGCAGCA GTGCGCTGTC TCCCGCCGTC AGCGGCATCA GTAAGGAGCT GGCAGAAATG 
CGGCACCTTG TTCAGTTCCC CGAGGAGATC GCCTGCATCC TCACTGAGCA GGAGCAGCAG 
CTCTACCAGC GGGTTTTCCC CCTGGACTAC CTCTGTTTCC TTACAAGAGA CCTGGGCAGT 
CCCGAGTGTC AAAGCAAACA TCACCCAAGC CTTAAGGCGT CACTTTCTGT GCCAGCCATG 
TCTACACAGA GCTCTCGACA CAATGCAGTG GAGGACCTGG TGGCACGCTT CAATGAGGTG 
AGCTCATGGG TAACCTGGCT GATTCTGACA GCAGGGTCCA TGGAAGAGAA GAGAGAGGTC 
TTCTCCTATC TGGTCAATGT TGCCAAGTGC TGTTGGAACA TGGGCAATTA CAATGGCGTC 
ATGGAGTTCC TGGCTGGACT CAGGTCCCGT AAGGTGCTGA AAATGTGGCA ATTTATGGAC 
CAGACTGACA TCGAGACCAT GCGTAGCCTG AAGGATGCTA TGGCTCAACA TGAATCCTCC 
TCTGAGTACA AGAAGGTTGT GACCAGAGCT CTCAACATCC CAGGATGTAA GGTGGTGCCT 
TTCTGTGGGG TCTTTCTAAA GGAGCTGAGT GATGCACTGG ATGGAACAGC AAGCATAATC 
AGCCTGAAAT CTCCTCTGGA GAATTCAGAA GACTCAATAG AGTTTGTTTC AGACTATAGC 
GGCCAGCACA ACTTCCTGTT GCGGTCTGGT CCTGATGGTC TACACATCCC AGAGAAAGAA 
GCTACTGTCA GTAACATTCT CCAGATTATC AGATCCTGTA ATCGTAGTTT GGAGGTGGAA 
GACACTGATG ATGGCTCAAC CAGCCCTTCT TCTAGTTGTC CACTCCCCAC TTCCAGAAAC 
AACTCCTTCA AGGACCGCTG CCGTAATCAT TCACGTTCAT TTAAGATGCC CCAAGAAACA 
AAACGTGCCT TGTTCATGGT GGGAGACTTG TCAGATTCAG ACGGAGACCT GCCATCTGAG 
CCTGCAGTGA AAGAGGGGGA GTTTCAGGGG ACTGAAGAAA CACACAAGGC TTTCAACCAT 
GGCACCGAGC TCATTCCCTG GTACGTGTTA TCGCTGCAGC CGGATGTTCA CCAGTTTCTG 
CTGCAGGGGG CCACGGTCAT CCACTACGAC CAGGACAGCC ACCTCTCAGC ACGCTGTCTG 
CTGCGGCTGC AGCCCGACAA CACAACGCTC ACCTGGGGTA ACCCACAGAA AGGTGGGGCC 
TCCCCCACAG AGCCACCACT GGGATTAGGA CAGGCCGTCG TCGCAGGACT AGCCGAGGGG 
CTTTTGGACC TTGGAGTGGT GAAGGCAGTG TTCCTAGGTC ATCAGGGAGT CGATGTTCAT 
GCTGTATGTC TGCAAAACAA GCTGAGCCAC ATGACAGTGG AGGAGAACAC CCTGAGCCTC 
CTCTATGGTG TCAGTACCAC TGACAACAGA CTTCTGCACT TTGTGGCTCC AAACCACACC 
ACGCAAATGC TCCATAAAGG TTTGTCAGAG TTGGTGACGG CCACACGGAA ATTGAAGAAG 
TTTCCTGATC AGCGGTTGCA GTGGCTGCGG AGGCAGTATG TCAGTTTATA TCAGGAGGAT 
GGCAGGTATG AAGGGCCCAC ATTAGCCCAA GCCATTGAGC TGTTTGGAGG CCGGAGATGG 
AACATGAGCA CAGGTGGAAC AGAGAAATCT GCCCATCAGA AAAACAGTCC TTTGAGCATC 
AACGATAAGA CCAAGAAGAA GAAGAAGGTC CTCGTCAGGG GAGACAGTGG AGATGCCACT 
GACGATGAGA TGGTTGCTCG GAAAACACGG AGCTGTAAGG AAGGAACATA TCGAAACGGA 
CCGGAGTCTG ACAGTATAGA TCACGAAGAC CCAGAAGATA GCTTCCCGGG ACCATTCTCC 
CTCTCAACCA ATAAAAGTCC TGGATTGCAA GCCTCTTCTT CTTCCTCCTC CTCCTCTTCC 
AGCATGACTG GCTCCAATCA GTCTCGCCCA CAGTCCTCCC CAACCCTCTC AGGAACAGTG 
AAAGCACAGC CAGGGGCGTG GAGCAGCAGG TCATGGCATG GTCGTGGTAA AGGCTGCTTC 
AGAGGCTTCC AGAACCTCAT GATCTCTGAC AGCACAATGA GCTTTGTTGA GTTTGTGGAG 
CTTTTCAAGT CATTCAGTAT CCGAAGCAGG AAGGATCTGA AGGAACTGTT TGACACCTTT 
GCTGTGCCCT GCATTCGCTC AGATCCAGAG TCTGTTCCTC TTTACACCAA TCTCAGGATA 
GATGACAAAG ACACAGGTCT ACAGCCGGAC CTCGATTTAT TAACGCGCAA TGGTTCGGAT 
CTTGGTCTGT TTATCCGAAC GAGGCAGCAG ATGTCTGACA ACCAGAAGCA GATCTCAGAC 
GCCATTGCAG CAGCCAGCAT TGTGACCAAC GGGACGGGAG TAGAAAACGC ATCACTGGGG 
GTTTTGGGAC TGGGCATCCC TCAACTTAAC GACTTCTTGG TCAACTGTCA GAGAGAACAT 
CTGAGCTATG ATGAGATCCT CAGCATTATA CAGAAGTTTG AACCCTCATC AAGCATGAGA 
CAAATGGGGT GGATGTCATT CGAAGGCTTT TCAAGGTTCC TGATGGATAA GGATAACTTT 
GCCTCACACA TTGAGGAATC CCAGATGAAC CCGGAGGAAC TGCAGCATCC CCTGTCTTAT 
TACTACATTC AGTCTTCTCA CAATACTTAT CTGACTGGGC ACCAGCTTAA AGGGGAGTCC 
TCTGTTGAGC TTTACAGTCA GGTGCTTTTG CAAGGCTGCA GAAGTGTGGA GCTGGACTGT 
TGGGATGGAG ATGATGGCAT GCCGGTTATT TATCACGGAC ACACTCTTAC CACCAAGATA 
CCATTTAAGG ATGTGGTGGA AGCAATTAGC AGGTCTGCAT TTGTCAACTC CAACATGCCC 
GTCGTCCTGT CTATAGAGAA CCACTGCTCC CTCCCGCAGC AGCGCAAGAT GGCGGAAATC 
TTCAAGACGG TGTTTGGGGA GCGTCTGGTG ACACGCTTCC TCTTTGAAAG CGACTTCAGC 
GATGACCCTC ACCTGCCATC TCCACTGCAG CTGCAGGGGA GGATTCTCCT CAAGAATAAG 
AAGCTGAAAG CACACCAGGC TCCGGTGGAC ATACTCAAGC AGAAGGCTCA CCAGCTTGCC 
CACATGCAGG CCCAGGTTAG CAATGGTTCA CCAGGGGTTT CCTCTCCCGG GACCCAAACC 
AACGAGGACG AGGAAGAGGA AGAAGACGAG TACGACTATG ACTATGAGTC TCTCTCAGAT 
GACAACCTCC TGGATGACAA ACCTGAGGTG AAAAGCTCAG CTGACAAAGA GGAGCAGCCT 
GTGGATGAAA TACCCAAGAG AATGAAGAAG CCTGACAACA CTACGCAGAG TAAAGGAAAG 
GTGTTTGACA TGGAACTGGG CGAGGAGTTC TACCTTCCAC AGAACAAAAA GGAAAGTCGA 
CAGATCGCTC AGGAGCTGTC TGACCTCGTC ATCTACTGTC AGGCCGTGAA GTTCCCTGGT 
CTGTCCACGC TGTCTCCGGC TGGCTCCGGC AGAGGGAAAG ACCGTGGAAA GAGCAGGAAG 
TCCATATTTG GCACGGCTCC AGCTCGCTGC AGCGCAACAG GGGAGGTCAC CACTCCGAGT 
CGCACCCCTG GGAAAGGTGG CGCCGAGCGC CTGAGCTGGG AAGAGCAGCA AACGTCTCCT 
GTCCTCAGTC CACCCACCTC TCTCAGCGCC ATTATCCGTA CACCCAAGTG TTATCACATC 
TCCTCCGTCA ATGAGAACGC CGCCAAGCGA CTGTGCCGGC GTTACTCTCA GAAACTGATC 
CAGCACACCG TGTGTCAGCT GCTCAGGACT TACCCAGCAG CCACCAGGAT CGACTCCACA 
AACCCAAACC CTCTGCTGTT CTGGCTGCAT GGCATTCAGC TGGTGGCTCT CAACTATCAG 
ACTGACGACC TGCCCATGCA GTTAAACACA GCTCTGTTTG AGGCCAACGG CGGCTGCGGC 
TATGTCCTGA AACCAGCGGT GCTGTGGGAC CGAAACTGTC CGATGTATCA GCAGTTCTGT 
CCGATGGAGA GGGATGTGGA AAAAATGAGT CCTGCCGTCT ACTCCCTTGC TATCGTTTCG 
GGGCAGAACG TCTGTCCCGG TAACAGCAGC GGCAGCCCCT GCATCGAGGT GGACGTCCTG 
GGGATGCCGG TCGACAGCGC CCACTTCCGT ACCAAACCCA TCCACCGCAA CACCCTCAAC 
CCCATGTGGA ACGAGCACTT TCAGTTCACC GTGCACTTTG AAGAGATGTG CTTCTTGCGC 
GTTGCTGTTG TGGAGAACAA CAGTTCCCAG ACTACTGCTC AGAGAACTCT ACCTCTGAAA 
GCCCTCAAGT CAGGTTACAG ACACATCCAG CTGAGGACTC AACACAATGA ATCTCTGGAA 
GTGTCAAGCT TGTTCATCTA TAGCCGAAGG ACAGAGGAGT GTCCGACAGG GGGCGACATT 
CCCTCATCCC TGCTGTTCAG CTCTGAGGAG AAGCCTGCAT CCCAGCAGCA CAGGGTGACG 
GTGTACGGAG CTCCTGGTCC TGAGCCCTTC ACTGTGTTCA GTGTGACGGA GCAGATCACT 
GCCAAACAGC TGCTGGACAT GGTCGTAGCT TCTGCTGGTG GAAATCCCTA CTTCCTGTGT 
GAGGAGAAGG TTCCTTTGAC GAAGGAGCGC AGTGAGACAA AACGCTGTGC TCAGTATCGC 
CCCCTTGCTC CGGAGGAGGA GGTGGTCCGA CTGGTTTCCA GCTGGAGTGC CGAGGAGGGA 
TATGTGGGAA GGATCTGCTT CAAGCTCAGA GAGGAGAAAT TAAATGAGAA GAACGCAGCA 
CCAGAAGGAG AGGAGGAGTG GAGTGTGGGC GGAGCCAGGG AGGGAGCTGG TGGAGAAGGA 
GCAGCAGCAG CAGAGGATGA TGTGTTCCTG GTCCAGATCC ATGAAGTTTC TCCAGAGCAG 
CCTCACACTG TGATCAAGGC CCCGCGCTAC AGCACAGCTC AGGACATCAT CCAGCAGACG 
TTATTCAAGG CCAAGTATTC CTACAGCATC CTCTCCAATC CCAATCCATG CGACTACGTC 
CTAATGGAGG AAGTAACCAA AGACGTGGGC TCAAAGAAGT CCTCCACCAC TAAGCCCCTG 
CAGCGGATGC TGCTGGATCA CGAGTGCGTC TATCAGGCTC AGAGCCGCTG GCGGGGCGCG 
GGGAAGTTCA TTCTGAAACT GAAAGAGCAG TTGGTGCGGG AAGACAAAAA GAAAGTCATT 
TCCTTTGCCA GTGAGCTGAA GAAACTGACG AGCCGCGGCC GCAGCATGAC GACTGGCGGC 
GGCGTGGACG GCCCCGGCCA GAGGTGTTGG TCTGCAGCAG TCGGTCTGAA GGTGTGGGTG 
GCAGATGGAA GGGTGGGGGC AGGTAGATTC CTTCCATCTT CCTCCTCTGG GAGACGTCTT 
CATCCAATCC ACCTGCCCAC CTCCTCTCTG ACTGCACCAG TGTTGAAGCT GCACCTCCTC 
AGGAACTGGA AGATCCACAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plce1 ( XM_011606177.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011604479.1 Takifugu rubripes phospholipase C, epsilon 1 (plce1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo22207
Clone ID Related Accession (Same CDS sequence)	XM_011606177.1
Accession Version	XM_011606177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070902.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGCA GCAGTGCGCT GTCTCCCGCC GTCAGCGGCA TCAGTAAGGA GCTGGCAGAA 
ATGCGGCACC TTGTTCAGTT CCCCGAGGAG ATCGCCTGCA TCCTCACTGA GCAGGAGCAG 
CAGCTCTACC AGCGGGTTTT CCCCCTGGAC TACCTCTGTT TCCTTACAAG AGACCTGGGC 
AGTCCCGAGT GTCAAAGCAA ACATCACCCA AGCCTTAAGG CGTCACTTTC TGTGCCAGCC 
ATGTCTACAC AGAGCTCTCG ACACAATGCA GTGGAGGACC TGGTGGCACG CTTCAATGAG 
GTGAGCTCAT GGGTAACCTG GCTGATTCTG ACAGCAGGGT CCATGGAAGA GAAGAGAGAG 
GTCTTCTCCT ATCTGGTCAA TGTTGCCAAG TGCTGTTGGA ACATGGGCAA TTACAATGGC 
GTCATGGAGT TCCTGGCTGG ACTCAGGTCC CGTAAGGTGC TGAAAATGTG GCAATTTATG 
GACCAGACTG ACATCGAGAC CATGCGTAGC CTGAAGGATG CTATGGCTCA ACATGAATCC 
TCCTCTGAGT ACAAGAAGGT TGTGACCAGA GCTCTCAACA TCCCAGGATG TAAGGTGGTG 
CCTTTCTGTG GGGTCTTTCT AAAGGAGCTG AGTGATGCAC TGGATGGAAC AGCAAGCATA 
ATCAGCCTGA AATCTCCTCT GGAGAATTCA GAAGACTCAA TAGAGTTTGT TTCAGACTAT 
AGCGGCCAGC ACAACTTCCT GTTGCGGTCT GGTCCTGATG GTCTACACAT CCCAGAGAAA 
GAAGCTACTG TCAGTAACAT TCTCCAGATT ATCAGATCCT GTAATCGTAG TTTGGAGGTG 
GAAGACACTG ATGATGGCTC AACCAGCCCT TCTTCTAGTT GTCCACTCCC CACTTCCAGA 
AACAACTCCT TCAAGGACCG CTGCCGTAAT CATTCACGTT CATTTAAGAT GCCCCAAGAA 
ACAAAACGTG CCTTGTTCAT GGTGGGAGAC TTGTCAGATT CAGACGGAGA CCTGCCATCT 
GAGCCTGCAG TGAAAGAGGG GGAGTTTCAG GGGACTGAAG AAACACACAA GGCTTTCAAC 
CATGGCACCG AGCTCATTCC CTGGTACGTG TTATCGCTGC AGCCGGATGT TCACCAGTTT 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA GCCACCTCTC AGCACGCTGT 
CTGCTGCGGC TGCAGCCCGA CAACACAACG CTCACCTGGG GTAACCCACA GAAAGGTGGG 
GCCTCCCCCA CAGAGCCACC ACTGGGATTA GGACAGGCCG TCGTCGCAGG ACTAGCCGAG 
GGGCTTTTGG ACCTTGGAGT GGTGAAGGCA GTGTTCCTAG GTCATCAGGG AGTCGATGTT 
CATGCTGTAT GTCTGCAAAA CAAGCTGAGC CACATGACAG TGGAGGAGAA CACCCTGAGC 
CTCCTCTATG GTGTCAGTAC CACTGACAAC AGACTTCTGC ACTTTGTGGC TCCAAACCAC 
ACCACGCAAA TGCTCCATAA AGGTTTGTCA GAGTTGGTGA CGGCCACACG GAAATTGAAG 
AAGTTTCCTG ATCAGCGGTT GCAGTGGCTG CGGAGGCAGT ATGTCAGTTT ATATCAGGAG 
GATGGCAGGT ATGAAGGGCC CACATTAGCC CAAGCCATTG AGCTGTTTGG AGGCCGGAGA 
TGGAACATGA GCACAGGTGG AACAGAGAAA TCTGCCCATC AGAAAAACAG TCCTTTGAGC 
ATCAACGATA AGACCAAGAA GAAGAAGAAG GTCCTCGTCA GGGGAGACAG TGGAGATGCC 
ACTGACGATG AGATGGTTGC TCGGAAAACA CGGAGCTGTA AGGAAGGAAC ATATCGAAAC 
GGACCGGAGT CTGACAGTAT AGATCACGAA GACCCAGAAG ATAGCTTCCC GGGACCATTC 
TCCCTCTCAA CCAATAAAAG TCCTGGATTG CAAGCCTCTT CTTCTTCCTC CTCCTCCTCT 
TCCAGCATGA CTGGCTCCAA TCAGTCTCGC CCACAGTCCT CCCCAACCCT CTCAGGAACA 
GTGAAAGCAC AGCCAGGGGC GTGGAGCAGC AGGTCATGGC ATGGTCGTGG TAAAGGCTGC 
TTCAGAGGCT TCCAGAACCT CATGATCTCT GACAGCACAA TGAGCTTTGT TGAGTTTGTG 
GAGCTTTTCA AGTCATTCAG TATCCGAAGC AGGAAGGATC TGAAGGAACT GTTTGACACC 
TTTGCTGTGC CCTGCATTCG CTCAGATCCA GAGTCTGTTC CTCTTTACAC CAATCTCAGG 
ATAGATGACA AAGACACAGG TCTACAGCCG GACCTCGATT TATTAACGCG CAATGGTTCG 
GATCTTGGTC TGTTTATCCG AACGAGGCAG CAGATGTCTG ACAACCAGAA GCAGATCTCA 
GACGCCATTG CAGCAGCCAG CATTGTGACC AACGGGACGG GAGTAGAAAA CGCATCACTG 
GGGGTTTTGG GACTGGGCAT CCCTCAACTT AACGACTTCT TGGTCAACTG TCAGAGAGAA 
CATCTGAGCT ATGATGAGAT CCTCAGCATT ATACAGAAGT TTGAACCCTC ATCAAGCATG 
AGACAAATGG GGTGGATGTC ATTCGAAGGC TTTTCAAGGT TCCTGATGGA TAAGGATAAC 
TTTGCCTCAC ACATTGAGGA ATCCCAGATG AACCCGGAGG AACTGCAGCA TCCCCTGTCT 
TATTACTACA TTCAGTCTTC TCACAATACT TATCTGACTG GGCACCAGCT TAAAGGGGAG 
TCCTCTGTTG AGCTTTACAG TCAGGTGCTT TTGCAAGGCT GCAGAAGTGT GGAGCTGGAC 
TGTTGGGATG GAGATGATGG CATGCCGGTT ATTTATCACG GACACACTCT TACCACCAAG 
ATACCATTTA AGGATGTGGT GGAAGCAATT AGCAGGTCTG CATTTGTCAA CTCCAACATG 
CCCGTCGTCC TGTCTATAGA GAACCACTGC TCCCTCCCGC AGCAGCGCAA GATGGCGGAA 
ATCTTCAAGA CGGTGTTTGG GGAGCGTCTG GTGACACGCT TCCTCTTTGA AAGCGACTTC 
AGCGATGACC CTCACCTGCC ATCTCCACTG CAGCTGCAGG GGAGGATTCT CCTCAAGAAT 
AAGAAGCTGA AAGCACACCA GGCTCCGGTG GACATACTCA AGCAGAAGGC TCACCAGCTT 
GCCCACATGC AGGCCCAGGT TAGCAATGGT TCACCAGGGG TTTCCTCTCC CGGGACCCAA 
ACCAACGAGG ACGAGGAAGA GGAAGAAGAC GAGTACGACT ATGACTATGA GTCTCTCTCA 
GATGACAACC TCCTGGATGA CAAACCTGAG GTGAAAAGCT CAGCTGACAA AGAGGAGCAG 
CCTGTGGATG AAATACCCAA GAGAATGAAG AAGCCTGACA ACACTACGCA GAGTAAAGGA 
AAGGTGTTTG ACATGGAACT GGGCGAGGAG TTCTACCTTC CACAGAACAA AAAGGAAAGT 
CGACAGATCG CTCAGGAGCT GTCTGACCTC GTCATCTACT GTCAGGCCGT GAAGTTCCCT 
GGTCTGTCCA CGCTGTCTCC GGCTGGCTCC GGCAGAGGGA AAGACCGTGG AAAGAGCAGG 
AAGTCCATAT TTGGCACGGC TCCAGCTCGC TGCAGCGCAA CAGGGGAGGT CACCACTCCG 
AGTCGCACCC CTGGGAAAGG TGGCGCCGAG CGCCTGAGCT GGGAAGAGCA GCAAACGTCT 
CCTGTCCTCA GTCCACCCAC CTCTCTCAGC GCCATTATCC GTACACCCAA GTGTTATCAC 
ATCTCCTCCG TCAATGAGAA CGCCGCCAAG CGACTGTGCC GGCGTTACTC TCAGAAACTG 
ATCCAGCACA CCGTGTGTCA GCTGCTCAGG ACTTACCCAG CAGCCACCAG GATCGACTCC 
ACAAACCCAA ACCCTCTGCT GTTCTGGCTG CATGGCATTC AGCTGGTGGC TCTCAACTAT 
CAGACTGACG ACCTGCCCAT GCAGTTAAAC ACAGCTCTGT TTGAGGCCAA CGGCGGCTGC 
GGCTATGTCC TGAAACCAGC GGTGCTGTGG GACCGAAACT GTCCGATGTA TCAGCAGTTC 
TGTCCGATGG AGAGGGATGT GGAAAAAATG AGTCCTGCCG TCTACTCCCT TGCTATCGTT 
TCGGGGCAGA ACGTCTGTCC CGGTAACAGC AGCGGCAGCC CCTGCATCGA GGTGGACGTC 
CTGGGGATGC CGGTCGACAG CGCCCACTTC CGTACCAAAC CCATCCACCG CAACACCCTC 
AACCCCATGT GGAACGAGCA CTTTCAGTTC ACCGTGCACT TTGAAGAGAT GTGCTTCTTG 
CGCGTTGCTG TTGTGGAGAA CAACAGTTCC CAGACTACTG CTCAGAGAAC TCTACCTCTG 
AAAGCCCTCA AGTCAGGTTA CAGACACATC CAGCTGAGGA CTCAACACAA TGAATCTCTG 
GAAGTGTCAA GCTTGTTCAT CTATAGCCGA AGGACAGAGG AGTGTCCGAC AGGGGGCGAC 
ATTCCCTCAT CCCTGCTGTT CAGCTCTGAG GAGAAGCCTG CATCCCAGCA GCACAGGGTG 
ACGGTGTACG GAGCTCCTGG TCCTGAGCCC TTCACTGTGT TCAGTGTGAC GGAGCAGATC 
ACTGCCAAAC AGCTGCTGGA CATGGTCGTA GCTTCTGCTG GTGGAAATCC CTACTTCCTG 
TGTGAGGAGA AGGTTCCTTT GACGAAGGAG CGCAGTGAGA CAAAACGCTG TGCTCAGTAT 
CGCCCCCTTG CTCCGGAGGA GGAGGTGGTC CGACTGGTTT CCAGCTGGAG TGCCGAGGAG 
GGATATGTGG GAAGGATCTG CTTCAAGCTC AGAGAGGAGA AATTAAATGA GAAGAACGCA 
GCACCAGAAG GAGAGGAGGA GTGGAGTGTG GGCGGAGCCA GGGAGGGAGC TGGTGGAGAA 
GGAGCAGCAG CAGCAGAGGA TGATGTGTTC CTGGTCCAGA TCCATGAAGT TTCTCCAGAG 
CAGCCTCACA CTGTGATCAA GGCCCCGCGC TACAGCACAG CTCAGGACAT CATCCAGCAG 
ACGTTATTCA AGGCCAAGTA TTCCTACAGC ATCCTCTCCA ATCCCAATCC ATGCGACTAC 
GTCCTAATGG AGGAAGTAAC CAAAGACGTG GGCTCAAAGA AGTCCTCCAC CACTAAGCCC 
CTGCAGCGGA TGCTGCTGGA TCACGAGTGC GTCTATCAGG CTCAGAGCCG CTGGCGGGGC 
GCGGGGAAGT TCATTCTGAA ACTGAAAGAG CAGTTGGTGC GGGAAGACAA AAAGAAAGTC 
ATTTCCTTTG CCAGTGAGCT GAAGAAACTG ACGAGCCGCG GCCGCAGCAT GACGACTGGC 
GGCGGCGTGG ACGGCCCCGG CCAGAGGTGT TGGTCTGCAG CAGTCGGTCT GAAGGTGTGG 
GTGGCAGATG GAAGGGTGGG GGCAGGTAGA TTCCTTCCAT CTTCCTCCTC TGGGAGACGT 
CTTCATCCAA TCCACCTGCC CACCTCCTCT CTGACTGCAC CAGTGTTGAA GCTGCACCTC 
CTCAGGAACT GGAAGATCCA CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604479.1
CDS	1364..6910
Translation	MTSSSALSPAVSGISKELAEMRHLVQFPEEIACILTEQEQQLYQRVFPLDYLCFLTRDLGSPECQSKHHPSLKASLSVPAMSTQSSRHNAVEDLVARFNEVSSWVTWLILTAGSMEEKREVFSYLVNVAKCCWNMGNYNGVMEFLAGLRSRKVLKMWQFMDQTDIETMRSLKDAMAQHESSSEYKKVVTRALNIPGCKVVPFCGVFLKELSDALDGTASIISLKSPLENSEDSIEFVSDYSGQHNFLLRSGPDGLHIPEKEATVSNILQIIRSCNRSLEVEDTDDGSTSPSSSCPLPTSRNNSFKDRCRNHSRSFKMPQETKRALFMVGDLSDSDGDLPSEPAVKEGEFQGTEETHKAFNHGTELIPWYVLSLQPDVHQFLLQGATVIHYDQDSHLSARCLLRLQPDNTTLTWGNPQKGGASPTEPPLGLGQAVVAGLAEGLLDLGVVKAVFLGHQGVDVHAVCLQNKLSHMTVEENTLSLLYGVSTTDNRLLHFVAPNHTTQMLHKGLSELVTATRKLKKFPDQRLQWLRRQYVSLYQEDGRYEGPTLAQAIELFGGRRWNMSTGGTEKSAHQKNSPLSINDKTKKKKKVLVRGDSGDATDDEMVARKTRSCKEGTYRNGPESDSIDHEDPEDSFPGPFSLSTNKSPGLQASSSSSSSSSSMTGSNQSRPQSSPTLSGTVKAQPGAWSSRSWHGRGKGCFRGFQNLMISDSTMSFVEFVELFKSFSIRSRKDLKELFDTFAVPCIRSDPESVPLYTNLRIDDKDTGLQPDLDLLTRNGSDLGLFIRTRQQMSDNQKQISDAIAAASIVTNGTGVENASLGVLGLGIPQLNDFLVNCQREHLSYDEILSIIQKFEPSSSMRQMGWMSFEGFSRFLMDKDNFASHIEESQMNPEELQHPLSYYYIQSSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPVIYHGHTLTTKIPFKDVVEAISRSAFVNSNMPVVLSIENHCSLPQQRKMAEIFKTVFGERLVTRFLFESDFSDDPHLPSPLQLQGRILLKNKKLKAHQAPVDILKQKAHQLAHMQAQVSNGSPGVSSPGTQTNEDEEEEEDEYDYDYESLSDDNLLDDKPEVKSSADKEEQPVDEIPKRMKKPDNTTQSKGKVFDMELGEEFYLPQNKKESRQIAQELSDLVIYCQAVKFPGLSTLSPAGSGRGKDRGKSRKSIFGTAPARCSATGEVTTPSRTPGKGGAERLSWEEQQTSPVLSPPTSLSAIIRTPKCYHISSVNENAAKRLCRRYSQKLIQHTVCQLLRTYPAATRIDSTNPNPLLFWLHGIQLVALNYQTDDLPMQLNTALFEANGGCGYVLKPAVLWDRNCPMYQQFCPMERDVEKMSPAVYSLAIVSGQNVCPGNSSGSPCIEVDVLGMPVDSAHFRTKPIHRNTLNPMWNEHFQFTVHFEEMCFLRVAVVENNSSQTTAQRTLPLKALKSGYRHIQLRTQHNESLEVSSLFIYSRRTEECPTGGDIPSSLLFSSEEKPASQQHRVTVYGAPGPEPFTVFSVTEQITAKQLLDMVVASAGGNPYFLCEEKVPLTKERSETKRCAQYRPLAPEEEVVRLVSSWSAEEGYVGRICFKLREEKLNEKNAAPEGEEEWSVGGAREGAGGEGAAAAEDDVFLVQIHEVSPEQPHTVIKAPRYSTAQDIIQQTLFKAKYSYSILSNPNPCDYVLMEEVTKDVGSKKSSTTKPLQRMLLDHECVYQAQSRWRGAGKFILKLKEQLVREDKKKVISFASELKKLTSRGRSMTTGGGVDGPGQRCWSAAVGLKVWVADGRVGAGRFLPSSSSGRRLHPIHLPTSSLTAPVLKLHLLRNWKIHK

Target ORF information:

RefSeq Version	XM_011606177.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phospholipase C, epsilon 1 (plce1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606177.1

ORF Insert Sequence:

ATGACCAGCA GCAGTGCGCT GTCTCCCGCC GTCAGCGGCA TCAGTAAGGA GCTGGCAGAA 
ATGCGGCACC TTGTTCAGTT CCCCGAGGAG ATCGCCTGCA TCCTCACTGA GCAGGAGCAG 
CAGCTCTACC AGCGGGTTTT CCCCCTGGAC TACCTCTGTT TCCTTACAAG AGACCTGGGC 
AGTCCCGAGT GTCAAAGCAA ACATCACCCA AGCCTTAAGG CGTCACTTTC TGTGCCAGCC 
ATGTCTACAC AGAGCTCTCG ACACAATGCA GTGGAGGACC TGGTGGCACG CTTCAATGAG 
GTGAGCTCAT GGGTAACCTG GCTGATTCTG ACAGCAGGGT CCATGGAAGA GAAGAGAGAG 
GTCTTCTCCT ATCTGGTCAA TGTTGCCAAG TGCTGTTGGA ACATGGGCAA TTACAATGGC 
GTCATGGAGT TCCTGGCTGG ACTCAGGTCC CGTAAGGTGC TGAAAATGTG GCAATTTATG 
GACCAGACTG ACATCGAGAC CATGCGTAGC CTGAAGGATG CTATGGCTCA ACATGAATCC 
TCCTCTGAGT ACAAGAAGGT TGTGACCAGA GCTCTCAACA TCCCAGGATG TAAGGTGGTG 
CCTTTCTGTG GGGTCTTTCT AAAGGAGCTG AGTGATGCAC TGGATGGAAC AGCAAGCATA 
ATCAGCCTGA AATCTCCTCT GGAGAATTCA GAAGACTCAA TAGAGTTTGT TTCAGACTAT 
AGCGGCCAGC ACAACTTCCT GTTGCGGTCT GGTCCTGATG GTCTACACAT CCCAGAGAAA 
GAAGCTACTG TCAGTAACAT TCTCCAGATT ATCAGATCCT GTAATCGTAG TTTGGAGGTG 
GAAGACACTG ATGATGGCTC AACCAGCCCT TCTTCTAGTT GTCCACTCCC CACTTCCAGA 
AACAACTCCT TCAAGGACCG CTGCCGTAAT CATTCACGTT CATTTAAGAT GCCCCAAGAA 
ACAAAACGTG CCTTGTTCAT GGTGGGAGAC TTGTCAGATT CAGACGGAGA CCTGCCATCT 
GAGCCTGCAG TGAAAGAGGG GGAGTTTCAG GGGACTGAAG AAACACACAA GGCTTTCAAC 
CATGGCACCG AGCTCATTCC CTGGTACGTG TTATCGCTGC AGCCGGATGT TCACCAGTTT 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA GCCACCTCTC AGCACGCTGT 
CTGCTGCGGC TGCAGCCCGA CAACACAACG CTCACCTGGG GTAACCCACA GAAAGGTGGG 
GCCTCCCCCA CAGAGCCACC ACTGGGATTA GGACAGGCCG TCGTCGCAGG ACTAGCCGAG 
GGGCTTTTGG ACCTTGGAGT GGTGAAGGCA GTGTTCCTAG GTCATCAGGG AGTCGATGTT 
CATGCTGTAT GTCTGCAAAA CAAGCTGAGC CACATGACAG TGGAGGAGAA CACCCTGAGC 
CTCCTCTATG GTGTCAGTAC CACTGACAAC AGACTTCTGC ACTTTGTGGC TCCAAACCAC 
ACCACGCAAA TGCTCCATAA AGGTTTGTCA GAGTTGGTGA CGGCCACACG GAAATTGAAG 
AAGTTTCCTG ATCAGCGGTT GCAGTGGCTG CGGAGGCAGT ATGTCAGTTT ATATCAGGAG 
GATGGCAGGT ATGAAGGGCC CACATTAGCC CAAGCCATTG AGCTGTTTGG AGGCCGGAGA 
TGGAACATGA GCACAGGTGG AACAGAGAAA TCTGCCCATC AGAAAAACAG TCCTTTGAGC 
ATCAACGATA AGACCAAGAA GAAGAAGAAG GTCCTCGTCA GGGGAGACAG TGGAGATGCC 
ACTGACGATG AGATGGTTGC TCGGAAAACA CGGAGCTGTA AGGAAGGAAC ATATCGAAAC 
GGACCGGAGT CTGACAGTAT AGATCACGAA GACCCAGAAG ATAGCTTCCC GGGACCATTC 
TCCCTCTCAA CCAATAAAAG TCCTGGATTG CAAGCCTCTT CTTCTTCCTC CTCCTCCTCT 
TCCAGCATGA CTGGCTCCAA TCAGTCTCGC CCACAGTCCT CCCCAACCCT CTCAGGAACA 
GTGAAAGCAC AGCCAGGGGC GTGGAGCAGC AGGTCATGGC ATGGTCGTGG TAAAGGCTGC 
TTCAGAGGCT TCCAGAACCT CATGATCTCT GACAGCACAA TGAGCTTTGT TGAGTTTGTG 
GAGCTTTTCA AGTCATTCAG TATCCGAAGC AGGAAGGATC TGAAGGAACT GTTTGACACC 
TTTGCTGTGC CCTGCATTCG CTCAGATCCA GAGTCTGTTC CTCTTTACAC CAATCTCAGG 
ATAGATGACA AAGACACAGG TCTACAGCCG GACCTCGATT TATTAACGCG CAATGGTTCG 
GATCTTGGTC TGTTTATCCG AACGAGGCAG CAGATGTCTG ACAACCAGAA GCAGATCTCA 
GACGCCATTG CAGCAGCCAG CATTGTGACC AACGGGACGG GAGTAGAAAA CGCATCACTG 
GGGGTTTTGG GACTGGGCAT CCCTCAACTT AACGACTTCT TGGTCAACTG TCAGAGAGAA 
CATCTGAGCT ATGATGAGAT CCTCAGCATT ATACAGAAGT TTGAACCCTC ATCAAGCATG 
AGACAAATGG GGTGGATGTC ATTCGAAGGC TTTTCAAGGT TCCTGATGGA TAAGGATAAC 
TTTGCCTCAC ACATTGAGGA ATCCCAGATG AACCCGGAGG AACTGCAGCA TCCCCTGTCT 
TATTACTACA TTCAGTCTTC TCACAATACT TATCTGACTG GGCACCAGCT TAAAGGGGAG 
TCCTCTGTTG AGCTTTACAG TCAGGTGCTT TTGCAAGGCT GCAGAAGTGT GGAGCTGGAC 
TGTTGGGATG GAGATGATGG CATGCCGGTT ATTTATCACG GACACACTCT TACCACCAAG 
ATACCATTTA AGGATGTGGT GGAAGCAATT AGCAGGTCTG CATTTGTCAA CTCCAACATG 
CCCGTCGTCC TGTCTATAGA GAACCACTGC TCCCTCCCGC AGCAGCGCAA GATGGCGGAA 
ATCTTCAAGA CGGTGTTTGG GGAGCGTCTG GTGACACGCT TCCTCTTTGA AAGCGACTTC 
AGCGATGACC CTCACCTGCC ATCTCCACTG CAGCTGCAGG GGAGGATTCT CCTCAAGAAT 
AAGAAGCTGA AAGCACACCA GGCTCCGGTG GACATACTCA AGCAGAAGGC TCACCAGCTT 
GCCCACATGC AGGCCCAGGT TAGCAATGGT TCACCAGGGG TTTCCTCTCC CGGGACCCAA 
ACCAACGAGG ACGAGGAAGA GGAAGAAGAC GAGTACGACT ATGACTATGA GTCTCTCTCA 
GATGACAACC TCCTGGATGA CAAACCTGAG GTGAAAAGCT CAGCTGACAA AGAGGAGCAG 
CCTGTGGATG AAATACCCAA GAGAATGAAG AAGCCTGACA ACACTACGCA GAGTAAAGGA 
AAGGTGTTTG ACATGGAACT GGGCGAGGAG TTCTACCTTC CACAGAACAA AAAGGAAAGT 
CGACAGATCG CTCAGGAGCT GTCTGACCTC GTCATCTACT GTCAGGCCGT GAAGTTCCCT 
GGTCTGTCCA CGCTGTCTCC GGCTGGCTCC GGCAGAGGGA AAGACCGTGG AAAGAGCAGG 
AAGTCCATAT TTGGCACGGC TCCAGCTCGC TGCAGCGCAA CAGGGGAGGT CACCACTCCG 
AGTCGCACCC CTGGGAAAGG TGGCGCCGAG CGCCTGAGCT GGGAAGAGCA GCAAACGTCT 
CCTGTCCTCA GTCCACCCAC CTCTCTCAGC GCCATTATCC GTACACCCAA GTGTTATCAC 
ATCTCCTCCG TCAATGAGAA CGCCGCCAAG CGACTGTGCC GGCGTTACTC TCAGAAACTG 
ATCCAGCACA CCGTGTGTCA GCTGCTCAGG ACTTACCCAG CAGCCACCAG GATCGACTCC 
ACAAACCCAA ACCCTCTGCT GTTCTGGCTG CATGGCATTC AGCTGGTGGC TCTCAACTAT 
CAGACTGACG ACCTGCCCAT GCAGTTAAAC ACAGCTCTGT TTGAGGCCAA CGGCGGCTGC 
GGCTATGTCC TGAAACCAGC GGTGCTGTGG GACCGAAACT GTCCGATGTA TCAGCAGTTC 
TGTCCGATGG AGAGGGATGT GGAAAAAATG AGTCCTGCCG TCTACTCCCT TGCTATCGTT 
TCGGGGCAGA ACGTCTGTCC CGGTAACAGC AGCGGCAGCC CCTGCATCGA GGTGGACGTC 
CTGGGGATGC CGGTCGACAG CGCCCACTTC CGTACCAAAC CCATCCACCG CAACACCCTC 
AACCCCATGT GGAACGAGCA CTTTCAGTTC ACCGTGCACT TTGAAGAGAT GTGCTTCTTG 
CGCGTTGCTG TTGTGGAGAA CAACAGTTCC CAGACTACTG CTCAGAGAAC TCTACCTCTG 
AAAGCCCTCA AGTCAGGTTA CAGACACATC CAGCTGAGGA CTCAACACAA TGAATCTCTG 
GAAGTGTCAA GCTTGTTCAT CTATAGCCGA AGGACAGAGG AGTGTCCGAC AGGGGGCGAC 
ATTCCCTCAT CCCTGCTGTT CAGCTCTGAG GAGAAGCCTG CATCCCAGCA GCACAGGGTG 
ACGGTGTACG GAGCTCCTGG TCCTGAGCCC TTCACTGTGT TCAGTGTGAC GGAGCAGATC 
ACTGCCAAAC AGCTGCTGGA CATGGTCGTA GCTTCTGCTG GTGGAAATCC CTACTTCCTG 
TGTGAGGAGA AGGTTCCTTT GACGAAGGAG CGCAGTGAGA CAAAACGCTG TGCTCAGTAT 
CGCCCCCTTG CTCCGGAGGA GGAGGTGGTC CGACTGGTTT CCAGCTGGAG TGCCGAGGAG 
GGATATGTGG GAAGGATCTG CTTCAAGCTC AGAGAGGAGA AATTAAATGA GAAGAACGCA 
GCACCAGAAG GAGAGGAGGA GTGGAGTGTG GGCGGAGCCA GGGAGGGAGC TGGTGGAGAA 
GGAGCAGCAG CAGCAGAGGA TGATGTGTTC CTGGTCCAGA TCCATGAAGT TTCTCCAGAG 
CAGCCTCACA CTGTGATCAA GGCCCCGCGC TACAGCACAG CTCAGGACAT CATCCAGCAG 
ACGTTATTCA AGGCCAAGTA TTCCTACAGC ATCCTCTCCA ATCCCAATCC ATGCGACTAC 
GTCCTAATGG AGGAAGTAAC CAAAGACGTG GGCTCAAAGA AGTCCTCCAC CACTAAGCCC 
CTGCAGCGGA TGCTGCTGGA TCACGAGTGC GTCTATCAGG CTCAGAGCCG CTGGCGGGGC 
GCGGGGAAGT TCATTCTGAA ACTGAAAGAG CAGTTGGTGC GGGAAGACAA AAAGAAAGTC 
ATTTCCTTTG CCAGTGAGCT GAAGAAACTG ACGAGCCGCG GCCGCAGCAT GACGACTGGC 
GGCGGCGTGG ACGGCCCCGG CCAGAGGTGT TGGTCTGCAG CAGTCGGTCT GAAGGTGTGG 
GTGGCAGATG GAAGGGTGGG GGCAGGTAGA TTCCTTCCAT CTTCCTCCTC TGGGAGACGT 
CTTCATCCAA TCCACCTGCC CACCTCCTCT CTGACTGCAC CAGTGTTGAA GCTGCACCTC 
CTCAGGAACT GGAAGATCCA CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plce1 ( XM_011606185.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011604487.1 Takifugu rubripes phospholipase C, epsilon 1 (plce1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo22208
Clone ID Related Accession (Same CDS sequence)	XM_011606185.1
Accession Version	XM_011606185.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070902.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAAAT CAGTGTGTAC TGGGACAAGA GCGATTGTCC GAGCGCTGCC CTCAGGACAC 
ATATCAGAGG AGGTCTGGAA GTCGGTGGGC ACTTATAGAA ACTGGCGTCC TACTAAGAAG 
GAGATAGGAC CAATCCTGAT GTGTGGCCGA AGAAAGAAAG ATGAATTCAG AGTGTGCAGG 
GAAATGCAGC GTCTGGTCTG GCCTGGAATC ATGGACCAAA TGGTCACCTA CTATGTGCAG 
AACAGAAAGC AGTATCCTCT GAGTCATCCA TGCCAAAAGC TGCTGTTGTC TTGTCCTGGA 
TGTGTGTGTC TCAGGGTGTC TCTGTGCCCA GAGACCCCCA TCACTGACAG AGCGGACCTG 
AGCCCCTGCT GCAGACTGTG GCGCTGCCAT CCTGCTCACC CACCTGGGGT TTATCGTGCT 
TGCCTCCAGA GGACTTCGCT GTTTTCCCTG CTGACTGGAG CCCTGCTGGA GGCTATAGCA 
GCTCTTGGTG CCCGCTCTGC GTTACCCTAC TCTTTCCCTC AGGGCTCGAA CAGCCACGTC 
GTGCTGAAAG AGCGTCAGCT GGCCAACAGC ATGACCAGCA GCAGTGCGCT GTCTCCCGCC 
GTCAGCGGCA TCAGTAAGGA GCTGGCAGAA ATGCGGCACC TTGTTCAGTT CCCCGAGGAG 
ATCGCCTGCA TCCTCACTGA GCAGGAGCAG CAGCTCTACC AGCGGGTTTT CCCCCTGGAC 
TACCTCTGTT TCCTTACAAG AGACCTGGGC AGTCCCGAGT GTCAAAGCAA ACATCACCCA 
AGCCTTAAGG CGTCACTTTC TGTGCCAGCC ATGTCTACAC AGAGCTCTCG ACACAATGCA 
GTGGAGGACC TGGTGGCACG CTTCAATGAG GTGAGCTCAT GGGTAACCTG GCTGATTCTG 
ACAGCAGGGT CCATGGAAGA GAAGAGAGAG GTCTTCTCCT ATCTGGTCAA TGTTGCCAAG 
TGCTGTTGGA ACATGGGCAA TTACAATGGC GTCATGGAGT TCCTGGCTGG ACTCAGGTCC 
CGTAAGGTGC TGAAAATGTG GCAATTTATG GACCAGACTG ACATCGAGAC CATGCGTAGC 
CTGAAGGATG CTATGGCTCA ACATGAATCC TCCTCTGAGT ACAAGAAGGT TGTGACCAGA 
GCTCTCAACA TCCCAGGATG TAAGGTGGTG CCTTTCTGTG GGGTCTTTCT AAAGGAGCTG 
AGTGATGCAC TGGATGGAAC AGCAAGCATA ATCAGCCTGA AATCTCCTCT GGAGAATTCA 
GAAGACTCAA TAGAGTTTGT TTCAGACTAT AGCGGCCAGC ACAACTTCCT GTTGCGGTCT 
GGTCCTGATG GTCTACACAT CCCAGAGAAA GAAGCTACTG TCAGTAACAT TCTCCAGATT 
ATCAGATCCT GTAATCGTAG TTTGGAGGTG GAAGACACTG ATGATGGCTC AACCAGCCCT 
TCTTCTAGTT GTCCACTCCC CACTTCCAGA AACAACTCCT TCAAGGACCG CTGCCGTAAT 
CAGTTCATGG TGGGAGACTT GTCAGATTCA GACGGAGACC TGCCATCTGA GCCTGCAGTG 
AAAGAGGGGG AGTTTCAGGG GACTGAAGAA ACACACAAGG CTTTCAACCA TGGCACCGAG 
CTCATTCCCT GGTACGTGTT ATCGCTGCAG CCGGATGTTC ACCAGTTTCT GCTGCAGGGG 
GCCACGGTCA TCCACTACGA CCAGGACAGC CACCTCTCAG CACGCTGTCT GCTGCGGCTG 
CAGCCCGACA ACACAACGCT CACCTGGGGT AACCCACAGA AAGGTGGGGC CTCCCCCACA 
GAGCCACCAC TGGGATTAGG ACAGGCCGTC GTCGCAGGAC TAGCCGAGGG GCTTTTGGAC 
CTTGGAGTGG TGAAGGCAGT GTTCCTAGGT CATCAGGGAG TCGATGTTCA TGCTGTATGT 
CTGCAAAACA AGCTGAGCCA CATGACAGTG GAGGAGAACA CCCTGAGCCT CCTCTATGGT 
GTCAGTACCA CTGACAACAG ACTTCTGCAC TTTGTGGCTC CAAACCACAC CACGCAAATG 
CTCCATAAAG GTTTGTCAGA GTTGGTGACG GCCACACGGA AATTGAAGAA GTTTCCTGAT 
CAGCGGTTGC AGTGGCTGCG GAGGCAGTAT GTCAGTTTAT ATCAGGAGGA TGGCAGGTAT 
GAAGGGCCCA CATTAGCCCA AGCCATTGAG CTGTTTGGAG GCCGGAGATG GAACATGAGC 
ACAGGTGGAA CAGAGAAATC TGCCCATCAG AAAAACAGTC CTTTGAGCAT CAACGATAAG 
ACCAAGAAGA AGAAGAAGGT CCTCGTCAGG GGAGACAGTG GAGATGCCAC TGACGATGAG 
ATGGTTGCTC GGAAAACACG GAGCTGTAAG GAAGGAACAT ATCGAAACGG ACCGGAGTCT 
GACAGTATAG ATCACGAAGA CCCAGAAGAT AGCTTCCCGG GACCATTCTC CCTCTCAACC 
AATAAAAGTC CTGGATTGCA AGCCTCTTCT TCTTCCTCCT CCTCCTCTTC CAGCATGACT 
GGCTCCAATC AGTCTCGCCC ACAGTCCTCC CCAACCCTCT CAGGAACAGT GAAAGCACAG 
CCAGGGGCGT GGAGCAGCAG GTCATGGCAT GGTCGTGGTA AAGGCTGCTT CAGAGGCTTC 
CAGAACCTCA TGATCTCTGA CAGCACAATG AGCTTTGTTG AGTTTGTGGA GCTTTTCAAG 
TCATTCAGTA TCCGAAGCAG GAAGGATCTG AAGGAACTGT TTGACACCTT TGCTGTGCCC 
TGCATTCGCT CAGATCCAGA GTCTGTTCCT CTTTACACCA ATCTCAGGAT AGATGACAAA 
GACACAGGTC TACAGCCGGA CCTCGATTTA TTAACGCGCA ATGGTTCGGA TCTTGGTCTG 
TTTATCCGAA CGAGGCAGCA GATGTCTGAC AACCAGAAGC AGATCTCAGA CGCCATTGCA 
GCAGCCAGCA TTGTGACCAA CGGGACGGGA GTAGAAAACG CATCACTGGG GGTTTTGGGA 
CTGGGCATCC CTCAACTTAA CGACTTCTTG GTCAACTGTC AGAGAGAACA TCTGAGCTAT 
GATGAGATCC TCAGCATTAT ACAGAAGTTT GAACCCTCAT CAAGCATGAG ACAAATGGGG 
TGGATGTCAT TCGAAGGCTT TTCAAGGTTC CTGATGGATA AGGATAACTT TGCCTCACAC 
ATTGAGGAAT CCCAGATGAA CCCGGAGGAA CTGCAGCATC CCCTGTCTTA TTACTACATT 
CAGTCTTCTC ACAATACTTA TCTGACTGGG CACCAGCTTA AAGGGGAGTC CTCTGTTGAG 
CTTTACAGTC AGGTGCTTTT GCAAGGCTGC AGAAGTGTGG AGCTGGACTG TTGGGATGGA 
GATGATGGCA TGCCGGTTAT TTATCACGGA CACACTCTTA CCACCAAGAT ACCATTTAAG 
GATGTGGTGG AAGCAATTAG CAGGTCTGCA TTTGTCAACT CCAACATGCC CGTCGTCCTG 
TCTATAGAGA ACCACTGCTC CCTCCCGCAG CAGCGCAAGA TGGCGGAAAT CTTCAAGACG 
GTGTTTGGGG AGCGTCTGGT GACACGCTTC CTCTTTGAAA GCGACTTCAG CGATGACCCT 
CACCTGCCAT CTCCACTGCA GCTGCAGGGG AGGATTCTCC TCAAGAATAA GAAGCTGAAA 
GCACACCAGG CTCCGGTGGA CATACTCAAG CAGAAGGCTC ACCAGCTTGC CCACATGCAG 
GCCCAGGTTA GCAATGGTTC ACCAGGGGTT TCCTCTCCCG GGACCCAAAC CAACGAGGAC 
GAGGAAGAGG AAGAAGACGA GTACGACTAT GACTATGAGT CTCTCTCAGA TGACAACCTC 
CTGGATGACA AACCTGAGGT GAAAAGCTCA GCTGACAAAG AGGAGCAGCC TGTGGATGAA 
ATACCCAAGA GAATGAAGAA GCCTGACAAC ACTACGCAGA GTAAAGGAAA GGTGTTTGAC 
ATGGAACTGG GCGAGGAGTT CTACCTTCCA CAGAACAAAA AGGAAAGTCG ACAGATCGCT 
CAGGAGCTGT CTGACCTCGT CATCTACTGT CAGGCCGTGA AGTTCCCTGG TCTGTCCACG 
CTGTCTCCGG CTGGCTCCGG CAGAGGGAAA GACCGTGGAA AGAGCAGGAA GTCCATATTT 
GGCACGGCTC CAGCTCGCTG CAGCGCAACA GGGGAGGTCA CCACTCCGAG TCGCACCCCT 
GGGAAAGGTG GCGCCGAGCG CCTGAGCTGG GAAGAGCAGC AAACGTCTCC TGTCCTCAGT 
CCACCCACCT CTCTCAGCGC CATTATCCGT ACACCCAAGT GTTATCACAT CTCCTCCGTC 
AATGAGAACG CCGCCAAGCG ACTGTGCCGG CGTTACTCTC AGAAACTGAT CCAGCACACC 
GTGTGTCAGC TGCTCAGGAC TTACCCAGCA GCCACCAGGA TCGACTCCAC AAACCCAAAC 
CCTCTGCTGT TCTGGCTGCA TGGCATTCAG CTGGTGGCTC TCAACTATCA GACTGACGAC 
CTGCCCATGC AGTTAAACAC AGCTCTGTTT GAGGCCAACG GCGGCTGCGG CTATGTCCTG 
AAACCAGCGG TGCTGTGGGA CCGAAACTGT CCGATGTATC AGCAGTTCTG TCCGATGGAG 
AGGGATGTGG AAAAAATGAG TCCTGCCGTC TACTCCCTTG CTATCGTTTC GGGGCAGAAC 
GTCTGTCCCG GTAACAGCAG CGGCAGCCCC TGCATCGAGG TGGACGTCCT GGGGATGCCG 
GTCGACAGCG CCCACTTCCG TACCAAACCC ATCCACCGCA ACACCCTCAA CCCCATGTGG 
AACGAGCACT TTCAGTTCAC CGTGCACTTT GAAGAGATGT GCTTCTTGCG CGTTGCTGTT 
GTGGAGAACA ACAGTTCCCA GACTACTGCT CAGAGAACTC TACCTCTGAA AGCCCTCAAG 
TCAGGTTACA GACACATCCA GCTGAGGACT CAACACAATG AATCTCTGGA AGTGTCAAGC 
TTGTTCATCT ATAGCCGAAG GACAGAGGAG TGTCCGACAG GGGGCGACAT TCCCTCATCC 
CTGCTGTTCA GCTCTGAGGA GAAGCCTGCA TCCCAGCAGC ACAGGGTGAC GGTGTACGGA 
GCTCCTGGTC CTGAGCCCTT CACTGTGTTC AGTGTGACGG AGCAGATCAC TGCCAAACAG 
CTGCTGGACA TGGTCGTAGC TTCTGCTGGT GGAAATCCCT ACTTCCTGTG TGAGGAGAAG 
GTTCCTTTGA CGAAGGAGCG CAGTGAGACA AAACGCTGTG CTCAGTATCG CCCCCTTGCT 
CCGGAGGAGG AGGTGGTCCG ACTGGTTTCC AGCTGGAGTG CCGAGGAGGG ATATGTGGGA 
AGGATCTGCT TCAAGCTCAG AGAGGAGAAA TTAAATGAGA AGAACGCAGC ACCAGAAGGA 
GAGGAGGAGT GGAGTGTGGG CGGAGCCAGG GAGGGAGCTG GTGGAGAAGG AGCAGCAGCA 
GCAGAGGATG ATGTGTTCCT GGTCCAGATC CATGAAGTTT CTCCAGAGCA GCCTCACACT 
GTGATCAAGG CCCCGCGCTA CAGCACAGCT CAGGACATCA TCCAGCAGAC GTTATTCAAG 
GCCAAGTATT CCTACAGCAT CCTCTCCAAT CCCAATCCAT GCGACTACGT CCTAATGGAG 
GAAGTAACCA AAGACGTGGG CTCAAAGAAG TCCTCCACCA CTAAGCCCCT GCAGCGGATG 
CTGCTGGATC ACGAGTGCGT CTATCAGGCT CAGAGCCGCT GGCGGGGCGC GGGGAAGTTC 
ATTCTGAAAC TGAAAGAGCA GTTGGTGCGG GAAGACAAAA AGAAAGTCAT TTCCTTTGCC 
AGTGAGCTGA AGAAACTGAC GAGCCGCGGC CGCAGCATGA CGACTGGCGG CGGCGTGGAC 
GGCCCCGGCC AGAGTAAGGA TGATAGAGCT GCATGTTGTG TGGCTCTGAC AGAGCTCCAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604487.1
CDS	89..6034
Translation	MTKSVCTGTRAIVRALPSGHISEEVWKSVGTYRNWRPTKKEIGPILMCGRRKKDEFRVCREMQRLVWPGIMDQMVTYYVQNRKQYPLSHPCQKLLLSCPGCVCLRVSLCPETPITDRADLSPCCRLWRCHPAHPPGVYRACLQRTSLFSLLTGALLEAIAALGARSALPYSFPQGSNSHVVLKERQLANSMTSSSALSPAVSGISKELAEMRHLVQFPEEIACILTEQEQQLYQRVFPLDYLCFLTRDLGSPECQSKHHPSLKASLSVPAMSTQSSRHNAVEDLVARFNEVSSWVTWLILTAGSMEEKREVFSYLVNVAKCCWNMGNYNGVMEFLAGLRSRKVLKMWQFMDQTDIETMRSLKDAMAQHESSSEYKKVVTRALNIPGCKVVPFCGVFLKELSDALDGTASIISLKSPLENSEDSIEFVSDYSGQHNFLLRSGPDGLHIPEKEATVSNILQIIRSCNRSLEVEDTDDGSTSPSSSCPLPTSRNNSFKDRCRNQFMVGDLSDSDGDLPSEPAVKEGEFQGTEETHKAFNHGTELIPWYVLSLQPDVHQFLLQGATVIHYDQDSHLSARCLLRLQPDNTTLTWGNPQKGGASPTEPPLGLGQAVVAGLAEGLLDLGVVKAVFLGHQGVDVHAVCLQNKLSHMTVEENTLSLLYGVSTTDNRLLHFVAPNHTTQMLHKGLSELVTATRKLKKFPDQRLQWLRRQYVSLYQEDGRYEGPTLAQAIELFGGRRWNMSTGGTEKSAHQKNSPLSINDKTKKKKKVLVRGDSGDATDDEMVARKTRSCKEGTYRNGPESDSIDHEDPEDSFPGPFSLSTNKSPGLQASSSSSSSSSSMTGSNQSRPQSSPTLSGTVKAQPGAWSSRSWHGRGKGCFRGFQNLMISDSTMSFVEFVELFKSFSIRSRKDLKELFDTFAVPCIRSDPESVPLYTNLRIDDKDTGLQPDLDLLTRNGSDLGLFIRTRQQMSDNQKQISDAIAAASIVTNGTGVENASLGVLGLGIPQLNDFLVNCQREHLSYDEILSIIQKFEPSSSMRQMGWMSFEGFSRFLMDKDNFASHIEESQMNPEELQHPLSYYYIQSSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPVIYHGHTLTTKIPFKDVVEAISRSAFVNSNMPVVLSIENHCSLPQQRKMAEIFKTVFGERLVTRFLFESDFSDDPHLPSPLQLQGRILLKNKKLKAHQAPVDILKQKAHQLAHMQAQVSNGSPGVSSPGTQTNEDEEEEEDEYDYDYESLSDDNLLDDKPEVKSSADKEEQPVDEIPKRMKKPDNTTQSKGKVFDMELGEEFYLPQNKKESRQIAQELSDLVIYCQAVKFPGLSTLSPAGSGRGKDRGKSRKSIFGTAPARCSATGEVTTPSRTPGKGGAERLSWEEQQTSPVLSPPTSLSAIIRTPKCYHISSVNENAAKRLCRRYSQKLIQHTVCQLLRTYPAATRIDSTNPNPLLFWLHGIQLVALNYQTDDLPMQLNTALFEANGGCGYVLKPAVLWDRNCPMYQQFCPMERDVEKMSPAVYSLAIVSGQNVCPGNSSGSPCIEVDVLGMPVDSAHFRTKPIHRNTLNPMWNEHFQFTVHFEEMCFLRVAVVENNSSQTTAQRTLPLKALKSGYRHIQLRTQHNESLEVSSLFIYSRRTEECPTGGDIPSSLLFSSEEKPASQQHRVTVYGAPGPEPFTVFSVTEQITAKQLLDMVVASAGGNPYFLCEEKVPLTKERSETKRCAQYRPLAPEEEVVRLVSSWSAEEGYVGRICFKLREEKLNEKNAAPEGEEEWSVGGAREGAGGEGAAAAEDDVFLVQIHEVSPEQPHTVIKAPRYSTAQDIIQQTLFKAKYSYSILSNPNPCDYVLMEEVTKDVGSKKSSTTKPLQRMLLDHECVYQAQSRWRGAGKFILKLKEQLVREDKKKVISFASELKKLTSRGRSMTTGGGVDGPGQSKDDRAACCVALTELQE

Target ORF information:

RefSeq Version	XM_011606185.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phospholipase C, epsilon 1 (plce1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606185.1

ORF Insert Sequence:

ATGACTAAAT CAGTGTGTAC TGGGACAAGA GCGATTGTCC GAGCGCTGCC CTCAGGACAC 
ATATCAGAGG AGGTCTGGAA GTCGGTGGGC ACTTATAGAA ACTGGCGTCC TACTAAGAAG 
GAGATAGGAC CAATCCTGAT GTGTGGCCGA AGAAAGAAAG ATGAATTCAG AGTGTGCAGG 
GAAATGCAGC GTCTGGTCTG GCCTGGAATC ATGGACCAAA TGGTCACCTA CTATGTGCAG 
AACAGAAAGC AGTATCCTCT GAGTCATCCA TGCCAAAAGC TGCTGTTGTC TTGTCCTGGA 
TGTGTGTGTC TCAGGGTGTC TCTGTGCCCA GAGACCCCCA TCACTGACAG AGCGGACCTG 
AGCCCCTGCT GCAGACTGTG GCGCTGCCAT CCTGCTCACC CACCTGGGGT TTATCGTGCT 
TGCCTCCAGA GGACTTCGCT GTTTTCCCTG CTGACTGGAG CCCTGCTGGA GGCTATAGCA 
GCTCTTGGTG CCCGCTCTGC GTTACCCTAC TCTTTCCCTC AGGGCTCGAA CAGCCACGTC 
GTGCTGAAAG AGCGTCAGCT GGCCAACAGC ATGACCAGCA GCAGTGCGCT GTCTCCCGCC 
GTCAGCGGCA TCAGTAAGGA GCTGGCAGAA ATGCGGCACC TTGTTCAGTT CCCCGAGGAG 
ATCGCCTGCA TCCTCACTGA GCAGGAGCAG CAGCTCTACC AGCGGGTTTT CCCCCTGGAC 
TACCTCTGTT TCCTTACAAG AGACCTGGGC AGTCCCGAGT GTCAAAGCAA ACATCACCCA 
AGCCTTAAGG CGTCACTTTC TGTGCCAGCC ATGTCTACAC AGAGCTCTCG ACACAATGCA 
GTGGAGGACC TGGTGGCACG CTTCAATGAG GTGAGCTCAT GGGTAACCTG GCTGATTCTG 
ACAGCAGGGT CCATGGAAGA GAAGAGAGAG GTCTTCTCCT ATCTGGTCAA TGTTGCCAAG 
TGCTGTTGGA ACATGGGCAA TTACAATGGC GTCATGGAGT TCCTGGCTGG ACTCAGGTCC 
CGTAAGGTGC TGAAAATGTG GCAATTTATG GACCAGACTG ACATCGAGAC CATGCGTAGC 
CTGAAGGATG CTATGGCTCA ACATGAATCC TCCTCTGAGT ACAAGAAGGT TGTGACCAGA 
GCTCTCAACA TCCCAGGATG TAAGGTGGTG CCTTTCTGTG GGGTCTTTCT AAAGGAGCTG 
AGTGATGCAC TGGATGGAAC AGCAAGCATA ATCAGCCTGA AATCTCCTCT GGAGAATTCA 
GAAGACTCAA TAGAGTTTGT TTCAGACTAT AGCGGCCAGC ACAACTTCCT GTTGCGGTCT 
GGTCCTGATG GTCTACACAT CCCAGAGAAA GAAGCTACTG TCAGTAACAT TCTCCAGATT 
ATCAGATCCT GTAATCGTAG TTTGGAGGTG GAAGACACTG ATGATGGCTC AACCAGCCCT 
TCTTCTAGTT GTCCACTCCC CACTTCCAGA AACAACTCCT TCAAGGACCG CTGCCGTAAT 
CAGTTCATGG TGGGAGACTT GTCAGATTCA GACGGAGACC TGCCATCTGA GCCTGCAGTG 
AAAGAGGGGG AGTTTCAGGG GACTGAAGAA ACACACAAGG CTTTCAACCA TGGCACCGAG 
CTCATTCCCT GGTACGTGTT ATCGCTGCAG CCGGATGTTC ACCAGTTTCT GCTGCAGGGG 
GCCACGGTCA TCCACTACGA CCAGGACAGC CACCTCTCAG CACGCTGTCT GCTGCGGCTG 
CAGCCCGACA ACACAACGCT CACCTGGGGT AACCCACAGA AAGGTGGGGC CTCCCCCACA 
GAGCCACCAC TGGGATTAGG ACAGGCCGTC GTCGCAGGAC TAGCCGAGGG GCTTTTGGAC 
CTTGGAGTGG TGAAGGCAGT GTTCCTAGGT CATCAGGGAG TCGATGTTCA TGCTGTATGT 
CTGCAAAACA AGCTGAGCCA CATGACAGTG GAGGAGAACA CCCTGAGCCT CCTCTATGGT 
GTCAGTACCA CTGACAACAG ACTTCTGCAC TTTGTGGCTC CAAACCACAC CACGCAAATG 
CTCCATAAAG GTTTGTCAGA GTTGGTGACG GCCACACGGA AATTGAAGAA GTTTCCTGAT 
CAGCGGTTGC AGTGGCTGCG GAGGCAGTAT GTCAGTTTAT ATCAGGAGGA TGGCAGGTAT 
GAAGGGCCCA CATTAGCCCA AGCCATTGAG CTGTTTGGAG GCCGGAGATG GAACATGAGC 
ACAGGTGGAA CAGAGAAATC TGCCCATCAG AAAAACAGTC CTTTGAGCAT CAACGATAAG 
ACCAAGAAGA AGAAGAAGGT CCTCGTCAGG GGAGACAGTG GAGATGCCAC TGACGATGAG 
ATGGTTGCTC GGAAAACACG GAGCTGTAAG GAAGGAACAT ATCGAAACGG ACCGGAGTCT 
GACAGTATAG ATCACGAAGA CCCAGAAGAT AGCTTCCCGG GACCATTCTC CCTCTCAACC 
AATAAAAGTC CTGGATTGCA AGCCTCTTCT TCTTCCTCCT CCTCCTCTTC CAGCATGACT 
GGCTCCAATC AGTCTCGCCC ACAGTCCTCC CCAACCCTCT CAGGAACAGT GAAAGCACAG 
CCAGGGGCGT GGAGCAGCAG GTCATGGCAT GGTCGTGGTA AAGGCTGCTT CAGAGGCTTC 
CAGAACCTCA TGATCTCTGA CAGCACAATG AGCTTTGTTG AGTTTGTGGA GCTTTTCAAG 
TCATTCAGTA TCCGAAGCAG GAAGGATCTG AAGGAACTGT TTGACACCTT TGCTGTGCCC 
TGCATTCGCT CAGATCCAGA GTCTGTTCCT CTTTACACCA ATCTCAGGAT AGATGACAAA 
GACACAGGTC TACAGCCGGA CCTCGATTTA TTAACGCGCA ATGGTTCGGA TCTTGGTCTG 
TTTATCCGAA CGAGGCAGCA GATGTCTGAC AACCAGAAGC AGATCTCAGA CGCCATTGCA 
GCAGCCAGCA TTGTGACCAA CGGGACGGGA GTAGAAAACG CATCACTGGG GGTTTTGGGA 
CTGGGCATCC CTCAACTTAA CGACTTCTTG GTCAACTGTC AGAGAGAACA TCTGAGCTAT 
GATGAGATCC TCAGCATTAT ACAGAAGTTT GAACCCTCAT CAAGCATGAG ACAAATGGGG 
TGGATGTCAT TCGAAGGCTT TTCAAGGTTC CTGATGGATA AGGATAACTT TGCCTCACAC 
ATTGAGGAAT CCCAGATGAA CCCGGAGGAA CTGCAGCATC CCCTGTCTTA TTACTACATT 
CAGTCTTCTC ACAATACTTA TCTGACTGGG CACCAGCTTA AAGGGGAGTC CTCTGTTGAG 
CTTTACAGTC AGGTGCTTTT GCAAGGCTGC AGAAGTGTGG AGCTGGACTG TTGGGATGGA 
GATGATGGCA TGCCGGTTAT TTATCACGGA CACACTCTTA CCACCAAGAT ACCATTTAAG 
GATGTGGTGG AAGCAATTAG CAGGTCTGCA TTTGTCAACT CCAACATGCC CGTCGTCCTG 
TCTATAGAGA ACCACTGCTC CCTCCCGCAG CAGCGCAAGA TGGCGGAAAT CTTCAAGACG 
GTGTTTGGGG AGCGTCTGGT GACACGCTTC CTCTTTGAAA GCGACTTCAG CGATGACCCT 
CACCTGCCAT CTCCACTGCA GCTGCAGGGG AGGATTCTCC TCAAGAATAA GAAGCTGAAA 
GCACACCAGG CTCCGGTGGA CATACTCAAG CAGAAGGCTC ACCAGCTTGC CCACATGCAG 
GCCCAGGTTA GCAATGGTTC ACCAGGGGTT TCCTCTCCCG GGACCCAAAC CAACGAGGAC 
GAGGAAGAGG AAGAAGACGA GTACGACTAT GACTATGAGT CTCTCTCAGA TGACAACCTC 
CTGGATGACA AACCTGAGGT GAAAAGCTCA GCTGACAAAG AGGAGCAGCC TGTGGATGAA 
ATACCCAAGA GAATGAAGAA GCCTGACAAC ACTACGCAGA GTAAAGGAAA GGTGTTTGAC 
ATGGAACTGG GCGAGGAGTT CTACCTTCCA CAGAACAAAA AGGAAAGTCG ACAGATCGCT 
CAGGAGCTGT CTGACCTCGT CATCTACTGT CAGGCCGTGA AGTTCCCTGG TCTGTCCACG 
CTGTCTCCGG CTGGCTCCGG CAGAGGGAAA GACCGTGGAA AGAGCAGGAA GTCCATATTT 
GGCACGGCTC CAGCTCGCTG CAGCGCAACA GGGGAGGTCA CCACTCCGAG TCGCACCCCT 
GGGAAAGGTG GCGCCGAGCG CCTGAGCTGG GAAGAGCAGC AAACGTCTCC TGTCCTCAGT 
CCACCCACCT CTCTCAGCGC CATTATCCGT ACACCCAAGT GTTATCACAT CTCCTCCGTC 
AATGAGAACG CCGCCAAGCG ACTGTGCCGG CGTTACTCTC AGAAACTGAT CCAGCACACC 
GTGTGTCAGC TGCTCAGGAC TTACCCAGCA GCCACCAGGA TCGACTCCAC AAACCCAAAC 
CCTCTGCTGT TCTGGCTGCA TGGCATTCAG CTGGTGGCTC TCAACTATCA GACTGACGAC 
CTGCCCATGC AGTTAAACAC AGCTCTGTTT GAGGCCAACG GCGGCTGCGG CTATGTCCTG 
AAACCAGCGG TGCTGTGGGA CCGAAACTGT CCGATGTATC AGCAGTTCTG TCCGATGGAG 
AGGGATGTGG AAAAAATGAG TCCTGCCGTC TACTCCCTTG CTATCGTTTC GGGGCAGAAC 
GTCTGTCCCG GTAACAGCAG CGGCAGCCCC TGCATCGAGG TGGACGTCCT GGGGATGCCG 
GTCGACAGCG CCCACTTCCG TACCAAACCC ATCCACCGCA ACACCCTCAA CCCCATGTGG 
AACGAGCACT TTCAGTTCAC CGTGCACTTT GAAGAGATGT GCTTCTTGCG CGTTGCTGTT 
GTGGAGAACA ACAGTTCCCA GACTACTGCT CAGAGAACTC TACCTCTGAA AGCCCTCAAG 
TCAGGTTACA GACACATCCA GCTGAGGACT CAACACAATG AATCTCTGGA AGTGTCAAGC 
TTGTTCATCT ATAGCCGAAG GACAGAGGAG TGTCCGACAG GGGGCGACAT TCCCTCATCC 
CTGCTGTTCA GCTCTGAGGA GAAGCCTGCA TCCCAGCAGC ACAGGGTGAC GGTGTACGGA 
GCTCCTGGTC CTGAGCCCTT CACTGTGTTC AGTGTGACGG AGCAGATCAC TGCCAAACAG 
CTGCTGGACA TGGTCGTAGC TTCTGCTGGT GGAAATCCCT ACTTCCTGTG TGAGGAGAAG 
GTTCCTTTGA CGAAGGAGCG CAGTGAGACA AAACGCTGTG CTCAGTATCG CCCCCTTGCT 
CCGGAGGAGG AGGTGGTCCG ACTGGTTTCC AGCTGGAGTG CCGAGGAGGG ATATGTGGGA 
AGGATCTGCT TCAAGCTCAG AGAGGAGAAA TTAAATGAGA AGAACGCAGC ACCAGAAGGA 
GAGGAGGAGT GGAGTGTGGG CGGAGCCAGG GAGGGAGCTG GTGGAGAAGG AGCAGCAGCA 
GCAGAGGATG ATGTGTTCCT GGTCCAGATC CATGAAGTTT CTCCAGAGCA GCCTCACACT 
GTGATCAAGG CCCCGCGCTA CAGCACAGCT CAGGACATCA TCCAGCAGAC GTTATTCAAG 
GCCAAGTATT CCTACAGCAT CCTCTCCAAT CCCAATCCAT GCGACTACGT CCTAATGGAG 
GAAGTAACCA AAGACGTGGG CTCAAAGAAG TCCTCCACCA CTAAGCCCCT GCAGCGGATG 
CTGCTGGATC ACGAGTGCGT CTATCAGGCT CAGAGCCGCT GGCGGGGCGC GGGGAAGTTC 
ATTCTGAAAC TGAAAGAGCA GTTGGTGCGG GAAGACAAAA AGAAAGTCAT TTCCTTTGCC 
AGTGAGCTGA AGAAACTGAC GAGCCGCGGC CGCAGCATGA CGACTGGCGG CGGCGTGGAC 
GGCCCCGGCC AGAGTAAGGA TGATAGAGCT GCATGTTGTG TGGCTCTGAC AGAGCTCCAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plce1 ( XM_029841545.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029697405.1 Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo40717
Clone ID Related Accession (Same CDS sequence)	XM_029841545.1 , XM_029841511.1 , XM_029841478.1
Accession Version	XM_029841545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCATTCACG TTCATTTAAG 
ATGCCCCAAG AAACAAAACG TGCCTTGTTC ATGGTGGGAG ACTTGTCAGA TTCAGACGGA 
GACCTGCCAT CTGAGCCTGC AGTGAAAGAG GGGGAGTTTC AGGGGACTGA AGAAACACAC 
AAGGCTTTCA ACCATGGCAC CGAGCTCATT CCCTGGTACG TGTTATCGCT GCAGCCGGAT 
GTTCACCAGT TTCTGCTGCA GGGGGCCACG GTCATCCACT ACGACCAGGA CAGCCACCTC 
TCAGCACGCT GTCTGCTGCG GCTGCAGCCC GACAACACAA CGCTCACCTG GGGTAACCCA 
CAGAAAGGTG GGGCCTCCCC CACAGAGCCA CCACTGGGAT TAGGACAGGC CGTCGTCGCA 
GGACTAGCCG AGGGGCTTTT GGACCTTGGA GTGGTGAAGG CAGTGTTCCT GGGTCATCAG 
GGAGTCGATG TTCATGCTGT ATGTCTGCAA AACAAGCTGA GCCACATGAC AGTGGAGGAG 
AACACCCTGA GCCTCCTCTA TGGTGTCAGT ACCACTGACA ACAGACTTCT GCACTTTGTG 
GCTCCAAACC ACACCACGCA AATGCTCCAT AAAGGTTTGT CAGAGTTGGT GACGGCCACA 
CGAAAATTGA AGAAGTTTCC TGATCAGCGG TTGCAGTGGC TGCGGAGGCA GTATGTCAGT 
TTATATCAGG AGGATGGCAG GTATGAAGGG CCCACATTAG CCCAAGCCAT TGAGCTGTTT 
GGAGGCCGGA GATGGAACAT GAGCACAGGT GGAACAGAGA AATCTGCCCA TCAGAAAAAC 
AGTCCTTTGA GCATCAACGA TAAGACCAAG AAGAAGAAGA AGGTCCTCGT CAGGGGAGAC 
AGTGGAGATG CCACTGACGA TGAGATGGTT GCTCGGAAAA CACGGAGCTG TAAGGAAGGA 
ACATATCGAA ACGGACCGGA GTCTGACAGT ATAGATCACG AAGACCCAGA AGATAGCTTC 
CCGGGACCAT TCTCCCTCTC AACCAATAAA AGTCCTGGAT TGCAAGCCTC TTCTTCTTCC 
TCCTCCTCCT CTTCCAGCAT GACTGGCTCC AATCAGTCTC GCCCACAGTC CTCCCCAACC 
CTCTCAGGAA CAGTGAAAGC ACAGCCAGGG GCGTGGAGCA GCAGGTCATG GCATGGTCGT 
GGTAAAGGCT GCTTCAGAGG CTTCCAGAAC CTCATGATCT CTGACAGCAC AATGAGCTTT 
GTTGAGTTTG TGGAGCTTTT CAAGTCATTC AGTATCCGAA GCAGGAAGGA TCTGAAGGAA 
CTGTTTGACA CCTTTGCTGT GCCCTGCATT CGCTCAGATC CAGAGTCTGT TCCTCTTTAC 
ACCAATCTCA GGATAGATGA CAAAGACACA GGTCTACAGC CGGACCTCGA TTTATTAACG 
CGCAATGGTT CGGATCTTGG TCTGTTTATC CGAACGAGGC AGCAGATGTC TGACAACCAG 
AAGCAGATCT CAGACGCCAT TGCAGCAGCC AGCATTGTGA CCAACGGGAC GGGAGTAGAA 
AACGCATCAC TGGGGGTTTT GGGACTGGGC ATCCCTCAAC TTAACGACTT CTTGGTCAAC 
TGTCAGAGAG AACATCTGAG CTATGATGAG ATCCTCAGCA TTATACAGAA GTTTGAACCC 
TCATCAAGCA TGAGACAAAT GGGGTGGATG TCATTCGAAG GCTTTTCAAG GTTCCTGATG 
GATAAGGATA ACTTTGCCTC ACACATTGAG GAATCCCAGA TGAACCCGGA GGAACTGCAG 
CATCCCCTGT CTTATTACTA CATTCAGTCT TCTCACAATA CTTATCTGAC TGGGCACCAG 
CTTAAAGGGG AGTCCTCTGT TGAGCTTTAC AGTCAGGTGC TTTTGCAAGG CTGCAGAAGT 
GTGGAGCTGG ACTGTTGGGA TGGAGATGAT GGCATGCCGG TTATTTATCA CGGACACACT 
CTTACCACCA AGATACCATT TAAGGATGTG GTGGAAGCAA TTAGCAGGTC TGCATTTGTC 
AACTCCAACA TGCCCGTCGT CCTGTCTATA GAGAACCACT GCTCCCTCCC GCAGCAGCGC 
AAGATGGCGG AAATCTTCAA GACGGTGTTT GGGGAGCGTC TGGTGACACG CTTCCTCTTT 
GAAAGCGACT TCAGCGATGA CCCTCACCTG CCATCTCCAC TGCAGCTGCA GGGGAGGATT 
CTCCTCAAGA ATAAGAAGCT GAAAGCACAC CAGGCTCCGG TGGACATACT CAAGCAGAAG 
GCTCACCAGC TTGCCCACAT GCAGGCCCAG GTTAGCAATG GTTCACCAGG GGTTTCCTCT 
CCCGGGACCC AAACCAACGA GGACGAGGAA GAGGAAGAAG ACGAGTACGA CTATGACTAT 
GAGTCCCTCT CAGATGCTGA TGTCCTCACA GCTTCTTCTG CCTCATGTGG CCTGGAAGAC 
AACCTCCTGG ATGACAAACC TGAGGTGAAA AGCTCAGCTG ACAAAGAGGA GCAGCCTGTG 
GATGAAATAC CCAAGAGAAT GAAGAAGCCT GACAACACTA CGCAGAGTAA AGGAAAGGTG 
TTTGACATGG AACTGGGCGA GGAGTTCTAC CTTCCACAGA ACAAAAAGGA AAGTCGACAG 
ATCGCTCAGG AGCTGTCTGA CCTCGTCATC TACTGTCAGG CCGTGAAGTT CCCTGGTCTG 
TCCACGCTGT CTCCGGCTGG CTCCGGCAGA GGGAAAGACC GTGGAAAGAG CAGGAAGTCC 
ATATTTGGCA CGGCTCCAGC TCGCTGCAGC GCAACAGGGG AGGTCACCAC TCCGAGTCGC 
ACCCCTGGGA AAGGTGGCGC CGAGCGCCTG AGCTGGGAAG AGCAGCAAAC GTCTCCTGTC 
CTCAGTCCAC CCACCTCTCT CAGCGCCATT ATCCGTACAC CCAAGTGTTA TCACATCTCC 
TCCGTCAATG AGAACGCCGC CAAGCGACTG TGCCGGCGTT ACTCTCAGAA ACTGATCCAG 
CACACCGTGT GTCAGCTGCT CAGGACTTAC CCAGCAGCCA CCAGGATCGA CTCCACAAAC 
CCAAACCCTC TGCTGTTCTG GCTGCATGGC ATTCAGCTGG TGGCTCTCAA CTATCAGACT 
GACGACCTGC CCATGCAGTT AAACACAGCT CTGTTTGAGG CCAACGGCGG CTGCGGCTAT 
GTCCTGAAAC CAGCGGTGCT GTGGGACCGA AACTGTCCGA TGTATCAGCA GTTCTGTCCG 
ATGGAGAGGG ATGTGGAAAA AATGAGTCCC GCCGTCTACT CCCTTGCTAT CGTTTCGGGG 
CAGAACGTCT GTCCCGGTAA CAGCAGCGGC AGCCCCTGCA TCGAGGTGGA CGTCCTGGGG 
ATGCCGGTCG ACAGCGCCCA CTTCCGTACC AAACCCATCC ACCGCAACAC CCTCAACCCC 
ATGTGGAACG AGCACTTTCA GTTCACCGTG CACTTTGAAG AGATGTGCTT CTTGCGCGTT 
GCTGTTGTGG AGAACAACAG TTCCCAGACT ACTGCTCAGA GAACTCTACC TCTGAAAGCC 
CTCAAGTCAG GTTACAGACA CATCCAGCTG AGGACTCAAC ACAATGAATC TCTGGAAGTG 
TCAAGCTTGT TCATCTATAG CCGAAGGACA GAGGAGTGTC CGACAGGGGG CGACATTCCC 
TCATCCCTGC TGTTCAGCTC TGAGGAGAAG CCTGCATCCC AGCAGCACAG GGTGACGGTG 
TACGGAGCTC CTGGTCCTGA GCCCTTCACT GTGTTCAGTG TGACGGAGCA GATCACTGCC 
AAACAGCTGC TGGACATGGT CGTAGCTTCT GCTGGTGGAA ATCCCTACTT CCTGTGTGAG 
GAGAAGGTTC CTTTGACGAA GGAGCGCAGT GAGACAAAAC GCTGTGCTCA GTATCGCCCC 
CTTGCTCCGG AGGAGGAGGT GGTCCGACTG GTTTCCAGCT GGAGTGCCGA GGAGGGATAT 
GTGGGAAGGA TCTGCTTCAA GCTCAGAGAG GAGAAATTAA ATGAGAAGAA CGCAGCACCA 
GAAGGAGAGG AGGAGTGGAG TGTGGGCGGA GCCAGGGAGG GAGCTGGTGG AGAAGGAGCA 
GCAGCAGCAG AGGATGATGT GTTCCTGGTC CAGATCCATG AAGTTTCTCC AGAGCAGCCT 
CACACTGTGA TCAAGGCCCC GCGCTACAGC ACAGCTCAGG ACATCATCCA GCAGACGTTA 
TTCAAGGCCA AGTATTCCTA CAGCATCCTC TCCAATCCCA ATCCATGCGA CTACGTCCTA 
ATGGAGGAAG TAACCAAAGA CGTGGGCTCA AAGAAGTCCT CCACCACTAA GCCCCTGCAG 
CGGATGCTGC TGGATCACGA GTGCGTCTAT CAGGCTCAGA GCCGCTGGCG GGGCGCGGGG 
AAGTTCATTC TGAAACTGAA AGAGCAGTTG GTGCGGGAAG ACAAAAAGAA AGTCATTTCC 
TTTGCCAGTG AGCTGAAGAA ACTGACGAGC CGCGGCCGCA GCATGACGAC TGGCGGCGGC 
GTGGACGGCC CCGGCCAGAG GTGTTGGTCT GCAGCAGTCG GTCTGAAGGT GTGGGTGGCA 
GATGGAAGGG TGGGGGCAGG TAGATTCCTT CCATCTTCCT CCTCTGGGAG ACGTCTTCAT 
CCAATCCACC TGCCCACCTC CTCTCTGACT GCACCAGTGT TGAAGCTGCA CCTCCTCAGG 
AACTGGAAGA TCCACAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029697405.1
CDS	608..7768
Translation	MSTKASQTPAEAAVVDRCSTAPSSSSKPTREVRNTNANQELQRYYDQLWNSNQRSSPPLIFYRKLPCDGEKGLIPNEALSNACLDRSSGGCEEPYQELSTGTDGIANVVPLISSEGTPCQLDSPGEGGQADRNRTGSSHCSSTMSTPQMIPNIDCCLSHTSSHLSPVHSPCFGSSPTQQKQRRGRRSSLPVSMMAFHKTSCASASEPNSSVSSPCNSPLLNHQQRHREGHFQRYRSKDGYSSLERLNRRPRVNKHSLQKLFPRRASLQTVITTGRSGLSSSPGSRDCSEDSSGSGSDGDGDGDGEELLDHTEFIRNRKERSTVLVRRFYKNNQKMTKSVCTGTRAIVRALPSGHISEEVWKSVGTYRNWRPTKKEIGPILMCGRRKKDEFRVCREMQRLVWPGIMDQMVTYYVQNRKQYPLSHPCQKLLLSCPGCVCLRVSLCPETPITDRADLSPCCRLWRCHPAHPPGVYRACLQRTSLFSLLTGALLEAIAALGARSALPYSFPQGSNSHVVLKERQLANSMTSSNALSPAISGISKELAEMRHLVQFPEEIACILTEQEQQLYQRVFPLDYLCFLTRDLGSPECQSKHHPSLKASLSVPAMSTQSSRHNAVEDLVARFNEVSSWVTWLILTAGSMEEKREVFSYLVNVAKCCWNMGNYNGVMEFLAGLRSRKVLKMWQFMDQTDIETMRSLKDAMAQHESSSEYKKVVTRALNIPGCKVVPFCGVFLKELSDALDGTASIISLKSPLENSEDSIEFVSDYSGQHNFLLRSGPDGLHIPEKEATVSNILQIIRSCNRSLEVEDTDDGSTSPSSSCPLPTSRNNSFKDRCRNHSRSFKMPQETKRALFMVGDLSDSDGDLPSEPAVKEGEFQGTEETHKAFNHGTELIPWYVLSLQPDVHQFLLQGATVIHYDQDSHLSARCLLRLQPDNTTLTWGNPQKGGASPTEPPLGLGQAVVAGLAEGLLDLGVVKAVFLGHQGVDVHAVCLQNKLSHMTVEENTLSLLYGVSTTDNRLLHFVAPNHTTQMLHKGLSELVTATRKLKKFPDQRLQWLRRQYVSLYQEDGRYEGPTLAQAIELFGGRRWNMSTGGTEKSAHQKNSPLSINDKTKKKKKVLVRGDSGDATDDEMVARKTRSCKEGTYRNGPESDSIDHEDPEDSFPGPFSLSTNKSPGLQASSSSSSSSSSMTGSNQSRPQSSPTLSGTVKAQPGAWSSRSWHGRGKGCFRGFQNLMISDSTMSFVEFVELFKSFSIRSRKDLKELFDTFAVPCIRSDPESVPLYTNLRIDDKDTGLQPDLDLLTRNGSDLGLFIRTRQQMSDNQKQISDAIAAASIVTNGTGVENASLGVLGLGIPQLNDFLVNCQREHLSYDEILSIIQKFEPSSSMRQMGWMSFEGFSRFLMDKDNFASHIEESQMNPEELQHPLSYYYIQSSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPVIYHGHTLTTKIPFKDVVEAISRSAFVNSNMPVVLSIENHCSLPQQRKMAEIFKTVFGERLVTRFLFESDFSDDPHLPSPLQLQGRILLKNKKLKAHQAPVDILKQKAHQLAHMQAQVSNGSPGVSSPGTQTNEDEEEEEDEYDYDYESLSDADVLTASSASCGLEDNLLDDKPEVKSSADKEEQPVDEIPKRMKKPDNTTQSKGKVFDMELGEEFYLPQNKKESRQIAQELSDLVIYCQAVKFPGLSTLSPAGSGRGKDRGKSRKSIFGTAPARCSATGEVTTPSRTPGKGGAERLSWEEQQTSPVLSPPTSLSAIIRTPKCYHISSVNENAAKRLCRRYSQKLIQHTVCQLLRTYPAATRIDSTNPNPLLFWLHGIQLVALNYQTDDLPMQLNTALFEANGGCGYVLKPAVLWDRNCPMYQQFCPMERDVEKMSPAVYSLAIVSGQNVCPGNSSGSPCIEVDVLGMPVDSAHFRTKPIHRNTLNPMWNEHFQFTVHFEEMCFLRVAVVENNSSQTTAQRTLPLKALKSGYRHIQLRTQHNESLEVSSLFIYSRRTEECPTGGDIPSSLLFSSEEKPASQQHRVTVYGAPGPEPFTVFSVTEQITAKQLLDMVVASAGGNPYFLCEEKVPLTKERSETKRCAQYRPLAPEEEVVRLVSSWSAEEGYVGRICFKLREEKLNEKNAAPEGEEEWSVGGAREGAGGEGAAAAEDDVFLVQIHEVSPEQPHTVIKAPRYSTAQDIIQQTLFKAKYSYSILSNPNPCDYVLMEEVTKDVGSKKSSTTKPLQRMLLDHECVYQAQSRWRGAGKFILKLKEQLVREDKKKVISFASELKKLTSRGRSMTTGGGVDGPGQRCWSAAVGLKVWVADGRVGAGRFLPSSSSGRRLHPIHLPTSSLTAPVLKLHLLRNWKIHK

Target ORF information:

RefSeq Version	XM_029841545.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029841545.1

ORF Insert Sequence:

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCATTCACG TTCATTTAAG 
ATGCCCCAAG AAACAAAACG TGCCTTGTTC ATGGTGGGAG ACTTGTCAGA TTCAGACGGA 
GACCTGCCAT CTGAGCCTGC AGTGAAAGAG GGGGAGTTTC AGGGGACTGA AGAAACACAC 
AAGGCTTTCA ACCATGGCAC CGAGCTCATT CCCTGGTACG TGTTATCGCT GCAGCCGGAT 
GTTCACCAGT TTCTGCTGCA GGGGGCCACG GTCATCCACT ACGACCAGGA CAGCCACCTC 
TCAGCACGCT GTCTGCTGCG GCTGCAGCCC GACAACACAA CGCTCACCTG GGGTAACCCA 
CAGAAAGGTG GGGCCTCCCC CACAGAGCCA CCACTGGGAT TAGGACAGGC CGTCGTCGCA 
GGACTAGCCG AGGGGCTTTT GGACCTTGGA GTGGTGAAGG CAGTGTTCCT GGGTCATCAG 
GGAGTCGATG TTCATGCTGT ATGTCTGCAA AACAAGCTGA GCCACATGAC AGTGGAGGAG 
AACACCCTGA GCCTCCTCTA TGGTGTCAGT ACCACTGACA ACAGACTTCT GCACTTTGTG 
GCTCCAAACC ACACCACGCA AATGCTCCAT AAAGGTTTGT CAGAGTTGGT GACGGCCACA 
CGAAAATTGA AGAAGTTTCC TGATCAGCGG TTGCAGTGGC TGCGGAGGCA GTATGTCAGT 
TTATATCAGG AGGATGGCAG GTATGAAGGG CCCACATTAG CCCAAGCCAT TGAGCTGTTT 
GGAGGCCGGA GATGGAACAT GAGCACAGGT GGAACAGAGA AATCTGCCCA TCAGAAAAAC 
AGTCCTTTGA GCATCAACGA TAAGACCAAG AAGAAGAAGA AGGTCCTCGT CAGGGGAGAC 
AGTGGAGATG CCACTGACGA TGAGATGGTT GCTCGGAAAA CACGGAGCTG TAAGGAAGGA 
ACATATCGAA ACGGACCGGA GTCTGACAGT ATAGATCACG AAGACCCAGA AGATAGCTTC 
CCGGGACCAT TCTCCCTCTC AACCAATAAA AGTCCTGGAT TGCAAGCCTC TTCTTCTTCC 
TCCTCCTCCT CTTCCAGCAT GACTGGCTCC AATCAGTCTC GCCCACAGTC CTCCCCAACC 
CTCTCAGGAA CAGTGAAAGC ACAGCCAGGG GCGTGGAGCA GCAGGTCATG GCATGGTCGT 
GGTAAAGGCT GCTTCAGAGG CTTCCAGAAC CTCATGATCT CTGACAGCAC AATGAGCTTT 
GTTGAGTTTG TGGAGCTTTT CAAGTCATTC AGTATCCGAA GCAGGAAGGA TCTGAAGGAA 
CTGTTTGACA CCTTTGCTGT GCCCTGCATT CGCTCAGATC CAGAGTCTGT TCCTCTTTAC 
ACCAATCTCA GGATAGATGA CAAAGACACA GGTCTACAGC CGGACCTCGA TTTATTAACG 
CGCAATGGTT CGGATCTTGG TCTGTTTATC CGAACGAGGC AGCAGATGTC TGACAACCAG 
AAGCAGATCT CAGACGCCAT TGCAGCAGCC AGCATTGTGA CCAACGGGAC GGGAGTAGAA 
AACGCATCAC TGGGGGTTTT GGGACTGGGC ATCCCTCAAC TTAACGACTT CTTGGTCAAC 
TGTCAGAGAG AACATCTGAG CTATGATGAG ATCCTCAGCA TTATACAGAA GTTTGAACCC 
TCATCAAGCA TGAGACAAAT GGGGTGGATG TCATTCGAAG GCTTTTCAAG GTTCCTGATG 
GATAAGGATA ACTTTGCCTC ACACATTGAG GAATCCCAGA TGAACCCGGA GGAACTGCAG 
CATCCCCTGT CTTATTACTA CATTCAGTCT TCTCACAATA CTTATCTGAC TGGGCACCAG 
CTTAAAGGGG AGTCCTCTGT TGAGCTTTAC AGTCAGGTGC TTTTGCAAGG CTGCAGAAGT 
GTGGAGCTGG ACTGTTGGGA TGGAGATGAT GGCATGCCGG TTATTTATCA CGGACACACT 
CTTACCACCA AGATACCATT TAAGGATGTG GTGGAAGCAA TTAGCAGGTC TGCATTTGTC 
AACTCCAACA TGCCCGTCGT CCTGTCTATA GAGAACCACT GCTCCCTCCC GCAGCAGCGC 
AAGATGGCGG AAATCTTCAA GACGGTGTTT GGGGAGCGTC TGGTGACACG CTTCCTCTTT 
GAAAGCGACT TCAGCGATGA CCCTCACCTG CCATCTCCAC TGCAGCTGCA GGGGAGGATT 
CTCCTCAAGA ATAAGAAGCT GAAAGCACAC CAGGCTCCGG TGGACATACT CAAGCAGAAG 
GCTCACCAGC TTGCCCACAT GCAGGCCCAG GTTAGCAATG GTTCACCAGG GGTTTCCTCT 
CCCGGGACCC AAACCAACGA GGACGAGGAA GAGGAAGAAG ACGAGTACGA CTATGACTAT 
GAGTCCCTCT CAGATGCTGA TGTCCTCACA GCTTCTTCTG CCTCATGTGG CCTGGAAGAC 
AACCTCCTGG ATGACAAACC TGAGGTGAAA AGCTCAGCTG ACAAAGAGGA GCAGCCTGTG 
GATGAAATAC CCAAGAGAAT GAAGAAGCCT GACAACACTA CGCAGAGTAA AGGAAAGGTG 
TTTGACATGG AACTGGGCGA GGAGTTCTAC CTTCCACAGA ACAAAAAGGA AAGTCGACAG 
ATCGCTCAGG AGCTGTCTGA CCTCGTCATC TACTGTCAGG CCGTGAAGTT CCCTGGTCTG 
TCCACGCTGT CTCCGGCTGG CTCCGGCAGA GGGAAAGACC GTGGAAAGAG CAGGAAGTCC 
ATATTTGGCA CGGCTCCAGC TCGCTGCAGC GCAACAGGGG AGGTCACCAC TCCGAGTCGC 
ACCCCTGGGA AAGGTGGCGC CGAGCGCCTG AGCTGGGAAG AGCAGCAAAC GTCTCCTGTC 
CTCAGTCCAC CCACCTCTCT CAGCGCCATT ATCCGTACAC CCAAGTGTTA TCACATCTCC 
TCCGTCAATG AGAACGCCGC CAAGCGACTG TGCCGGCGTT ACTCTCAGAA ACTGATCCAG 
CACACCGTGT GTCAGCTGCT CAGGACTTAC CCAGCAGCCA CCAGGATCGA CTCCACAAAC 
CCAAACCCTC TGCTGTTCTG GCTGCATGGC ATTCAGCTGG TGGCTCTCAA CTATCAGACT 
GACGACCTGC CCATGCAGTT AAACACAGCT CTGTTTGAGG CCAACGGCGG CTGCGGCTAT 
GTCCTGAAAC CAGCGGTGCT GTGGGACCGA AACTGTCCGA TGTATCAGCA GTTCTGTCCG 
ATGGAGAGGG ATGTGGAAAA AATGAGTCCC GCCGTCTACT CCCTTGCTAT CGTTTCGGGG 
CAGAACGTCT GTCCCGGTAA CAGCAGCGGC AGCCCCTGCA TCGAGGTGGA CGTCCTGGGG 
ATGCCGGTCG ACAGCGCCCA CTTCCGTACC AAACCCATCC ACCGCAACAC CCTCAACCCC 
ATGTGGAACG AGCACTTTCA GTTCACCGTG CACTTTGAAG AGATGTGCTT CTTGCGCGTT 
GCTGTTGTGG AGAACAACAG TTCCCAGACT ACTGCTCAGA GAACTCTACC TCTGAAAGCC 
CTCAAGTCAG GTTACAGACA CATCCAGCTG AGGACTCAAC ACAATGAATC TCTGGAAGTG 
TCAAGCTTGT TCATCTATAG CCGAAGGACA GAGGAGTGTC CGACAGGGGG CGACATTCCC 
TCATCCCTGC TGTTCAGCTC TGAGGAGAAG CCTGCATCCC AGCAGCACAG GGTGACGGTG 
TACGGAGCTC CTGGTCCTGA GCCCTTCACT GTGTTCAGTG TGACGGAGCA GATCACTGCC 
AAACAGCTGC TGGACATGGT CGTAGCTTCT GCTGGTGGAA ATCCCTACTT CCTGTGTGAG 
GAGAAGGTTC CTTTGACGAA GGAGCGCAGT GAGACAAAAC GCTGTGCTCA GTATCGCCCC 
CTTGCTCCGG AGGAGGAGGT GGTCCGACTG GTTTCCAGCT GGAGTGCCGA GGAGGGATAT 
GTGGGAAGGA TCTGCTTCAA GCTCAGAGAG GAGAAATTAA ATGAGAAGAA CGCAGCACCA 
GAAGGAGAGG AGGAGTGGAG TGTGGGCGGA GCCAGGGAGG GAGCTGGTGG AGAAGGAGCA 
GCAGCAGCAG AGGATGATGT GTTCCTGGTC CAGATCCATG AAGTTTCTCC AGAGCAGCCT 
CACACTGTGA TCAAGGCCCC GCGCTACAGC ACAGCTCAGG ACATCATCCA GCAGACGTTA 
TTCAAGGCCA AGTATTCCTA CAGCATCCTC TCCAATCCCA ATCCATGCGA CTACGTCCTA 
ATGGAGGAAG TAACCAAAGA CGTGGGCTCA AAGAAGTCCT CCACCACTAA GCCCCTGCAG 
CGGATGCTGC TGGATCACGA GTGCGTCTAT CAGGCTCAGA GCCGCTGGCG GGGCGCGGGG 
AAGTTCATTC TGAAACTGAA AGAGCAGTTG GTGCGGGAAG ACAAAAAGAA AGTCATTTCC 
TTTGCCAGTG AGCTGAAGAA ACTGACGAGC CGCGGCCGCA GCATGACGAC TGGCGGCGGC 
GTGGACGGCC CCGGCCAGAG GTGTTGGTCT GCAGCAGTCG GTCTGAAGGT GTGGGTGGCA 
GATGGAAGGG TGGGGGCAGG TAGATTCCTT CCATCTTCCT CCTCTGGGAG ACGTCTTCAT 
CCAATCCACC TGCCCACCTC CTCTCTGACT GCACCAGTGT TGAAGCTGCA CCTCCTCAGG 
AACTGGAAGA TCCACAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plce1 ( XM_029841511.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029697371.1 Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo40717
Clone ID Related Accession (Same CDS sequence)	XM_029841545.1 , XM_029841511.1 , XM_029841478.1
Accession Version	XM_029841511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCATTCACG TTCATTTAAG 
ATGCCCCAAG AAACAAAACG TGCCTTGTTC ATGGTGGGAG ACTTGTCAGA TTCAGACGGA 
GACCTGCCAT CTGAGCCTGC AGTGAAAGAG GGGGAGTTTC AGGGGACTGA AGAAACACAC 
AAGGCTTTCA ACCATGGCAC CGAGCTCATT CCCTGGTACG TGTTATCGCT GCAGCCGGAT 
GTTCACCAGT TTCTGCTGCA GGGGGCCACG GTCATCCACT ACGACCAGGA CAGCCACCTC 
TCAGCACGCT GTCTGCTGCG GCTGCAGCCC GACAACACAA CGCTCACCTG GGGTAACCCA 
CAGAAAGGTG GGGCCTCCCC CACAGAGCCA CCACTGGGAT TAGGACAGGC CGTCGTCGCA 
GGACTAGCCG AGGGGCTTTT GGACCTTGGA GTGGTGAAGG CAGTGTTCCT GGGTCATCAG 
GGAGTCGATG TTCATGCTGT ATGTCTGCAA AACAAGCTGA GCCACATGAC AGTGGAGGAG 
AACACCCTGA GCCTCCTCTA TGGTGTCAGT ACCACTGACA ACAGACTTCT GCACTTTGTG 
GCTCCAAACC ACACCACGCA AATGCTCCAT AAAGGTTTGT CAGAGTTGGT GACGGCCACA 
CGAAAATTGA AGAAGTTTCC TGATCAGCGG TTGCAGTGGC TGCGGAGGCA GTATGTCAGT 
TTATATCAGG AGGATGGCAG GTATGAAGGG CCCACATTAG CCCAAGCCAT TGAGCTGTTT 
GGAGGCCGGA GATGGAACAT GAGCACAGGT GGAACAGAGA AATCTGCCCA TCAGAAAAAC 
AGTCCTTTGA GCATCAACGA TAAGACCAAG AAGAAGAAGA AGGTCCTCGT CAGGGGAGAC 
AGTGGAGATG CCACTGACGA TGAGATGGTT GCTCGGAAAA CACGGAGCTG TAAGGAAGGA 
ACATATCGAA ACGGACCGGA GTCTGACAGT ATAGATCACG AAGACCCAGA AGATAGCTTC 
CCGGGACCAT TCTCCCTCTC AACCAATAAA AGTCCTGGAT TGCAAGCCTC TTCTTCTTCC 
TCCTCCTCCT CTTCCAGCAT GACTGGCTCC AATCAGTCTC GCCCACAGTC CTCCCCAACC 
CTCTCAGGAA CAGTGAAAGC ACAGCCAGGG GCGTGGAGCA GCAGGTCATG GCATGGTCGT 
GGTAAAGGCT GCTTCAGAGG CTTCCAGAAC CTCATGATCT CTGACAGCAC AATGAGCTTT 
GTTGAGTTTG TGGAGCTTTT CAAGTCATTC AGTATCCGAA GCAGGAAGGA TCTGAAGGAA 
CTGTTTGACA CCTTTGCTGT GCCCTGCATT CGCTCAGATC CAGAGTCTGT TCCTCTTTAC 
ACCAATCTCA GGATAGATGA CAAAGACACA GGTCTACAGC CGGACCTCGA TTTATTAACG 
CGCAATGGTT CGGATCTTGG TCTGTTTATC CGAACGAGGC AGCAGATGTC TGACAACCAG 
AAGCAGATCT CAGACGCCAT TGCAGCAGCC AGCATTGTGA CCAACGGGAC GGGAGTAGAA 
AACGCATCAC TGGGGGTTTT GGGACTGGGC ATCCCTCAAC TTAACGACTT CTTGGTCAAC 
TGTCAGAGAG AACATCTGAG CTATGATGAG ATCCTCAGCA TTATACAGAA GTTTGAACCC 
TCATCAAGCA TGAGACAAAT GGGGTGGATG TCATTCGAAG GCTTTTCAAG GTTCCTGATG 
GATAAGGATA ACTTTGCCTC ACACATTGAG GAATCCCAGA TGAACCCGGA GGAACTGCAG 
CATCCCCTGT CTTATTACTA CATTCAGTCT TCTCACAATA CTTATCTGAC TGGGCACCAG 
CTTAAAGGGG AGTCCTCTGT TGAGCTTTAC AGTCAGGTGC TTTTGCAAGG CTGCAGAAGT 
GTGGAGCTGG ACTGTTGGGA TGGAGATGAT GGCATGCCGG TTATTTATCA CGGACACACT 
CTTACCACCA AGATACCATT TAAGGATGTG GTGGAAGCAA TTAGCAGGTC TGCATTTGTC 
AACTCCAACA TGCCCGTCGT CCTGTCTATA GAGAACCACT GCTCCCTCCC GCAGCAGCGC 
AAGATGGCGG AAATCTTCAA GACGGTGTTT GGGGAGCGTC TGGTGACACG CTTCCTCTTT 
GAAAGCGACT TCAGCGATGA CCCTCACCTG CCATCTCCAC TGCAGCTGCA GGGGAGGATT 
CTCCTCAAGA ATAAGAAGCT GAAAGCACAC CAGGCTCCGG TGGACATACT CAAGCAGAAG 
GCTCACCAGC TTGCCCACAT GCAGGCCCAG GTTAGCAATG GTTCACCAGG GGTTTCCTCT 
CCCGGGACCC AAACCAACGA GGACGAGGAA GAGGAAGAAG ACGAGTACGA CTATGACTAT 
GAGTCCCTCT CAGATGCTGA TGTCCTCACA GCTTCTTCTG CCTCATGTGG CCTGGAAGAC 
AACCTCCTGG ATGACAAACC TGAGGTGAAA AGCTCAGCTG ACAAAGAGGA GCAGCCTGTG 
GATGAAATAC CCAAGAGAAT GAAGAAGCCT GACAACACTA CGCAGAGTAA AGGAAAGGTG 
TTTGACATGG AACTGGGCGA GGAGTTCTAC CTTCCACAGA ACAAAAAGGA AAGTCGACAG 
ATCGCTCAGG AGCTGTCTGA CCTCGTCATC TACTGTCAGG CCGTGAAGTT CCCTGGTCTG 
TCCACGCTGT CTCCGGCTGG CTCCGGCAGA GGGAAAGACC GTGGAAAGAG CAGGAAGTCC 
ATATTTGGCA CGGCTCCAGC TCGCTGCAGC GCAACAGGGG AGGTCACCAC TCCGAGTCGC 
ACCCCTGGGA AAGGTGGCGC CGAGCGCCTG AGCTGGGAAG AGCAGCAAAC GTCTCCTGTC 
CTCAGTCCAC CCACCTCTCT CAGCGCCATT ATCCGTACAC CCAAGTGTTA TCACATCTCC 
TCCGTCAATG AGAACGCCGC CAAGCGACTG TGCCGGCGTT ACTCTCAGAA ACTGATCCAG 
CACACCGTGT GTCAGCTGCT CAGGACTTAC CCAGCAGCCA CCAGGATCGA CTCCACAAAC 
CCAAACCCTC TGCTGTTCTG GCTGCATGGC ATTCAGCTGG TGGCTCTCAA CTATCAGACT 
GACGACCTGC CCATGCAGTT AAACACAGCT CTGTTTGAGG CCAACGGCGG CTGCGGCTAT 
GTCCTGAAAC CAGCGGTGCT GTGGGACCGA AACTGTCCGA TGTATCAGCA GTTCTGTCCG 
ATGGAGAGGG ATGTGGAAAA AATGAGTCCC GCCGTCTACT CCCTTGCTAT CGTTTCGGGG 
CAGAACGTCT GTCCCGGTAA CAGCAGCGGC AGCCCCTGCA TCGAGGTGGA CGTCCTGGGG 
ATGCCGGTCG ACAGCGCCCA CTTCCGTACC AAACCCATCC ACCGCAACAC CCTCAACCCC 
ATGTGGAACG AGCACTTTCA GTTCACCGTG CACTTTGAAG AGATGTGCTT CTTGCGCGTT 
GCTGTTGTGG AGAACAACAG TTCCCAGACT ACTGCTCAGA GAACTCTACC TCTGAAAGCC 
CTCAAGTCAG GTTACAGACA CATCCAGCTG AGGACTCAAC ACAATGAATC TCTGGAAGTG 
TCAAGCTTGT TCATCTATAG CCGAAGGACA GAGGAGTGTC CGACAGGGGG CGACATTCCC 
TCATCCCTGC TGTTCAGCTC TGAGGAGAAG CCTGCATCCC AGCAGCACAG GGTGACGGTG 
TACGGAGCTC CTGGTCCTGA GCCCTTCACT GTGTTCAGTG TGACGGAGCA GATCACTGCC 
AAACAGCTGC TGGACATGGT CGTAGCTTCT GCTGGTGGAA ATCCCTACTT CCTGTGTGAG 
GAGAAGGTTC CTTTGACGAA GGAGCGCAGT GAGACAAAAC GCTGTGCTCA GTATCGCCCC 
CTTGCTCCGG AGGAGGAGGT GGTCCGACTG GTTTCCAGCT GGAGTGCCGA GGAGGGATAT 
GTGGGAAGGA TCTGCTTCAA GCTCAGAGAG GAGAAATTAA ATGAGAAGAA CGCAGCACCA 
GAAGGAGAGG AGGAGTGGAG TGTGGGCGGA GCCAGGGAGG GAGCTGGTGG AGAAGGAGCA 
GCAGCAGCAG AGGATGATGT GTTCCTGGTC CAGATCCATG AAGTTTCTCC AGAGCAGCCT 
CACACTGTGA TCAAGGCCCC GCGCTACAGC ACAGCTCAGG ACATCATCCA GCAGACGTTA 
TTCAAGGCCA AGTATTCCTA CAGCATCCTC TCCAATCCCA ATCCATGCGA CTACGTCCTA 
ATGGAGGAAG TAACCAAAGA CGTGGGCTCA AAGAAGTCCT CCACCACTAA GCCCCTGCAG 
CGGATGCTGC TGGATCACGA GTGCGTCTAT CAGGCTCAGA GCCGCTGGCG GGGCGCGGGG 
AAGTTCATTC TGAAACTGAA AGAGCAGTTG GTGCGGGAAG ACAAAAAGAA AGTCATTTCC 
TTTGCCAGTG AGCTGAAGAA ACTGACGAGC CGCGGCCGCA GCATGACGAC TGGCGGCGGC 
GTGGACGGCC CCGGCCAGAG GTGTTGGTCT GCAGCAGTCG GTCTGAAGGT GTGGGTGGCA 
GATGGAAGGG TGGGGGCAGG TAGATTCCTT CCATCTTCCT CCTCTGGGAG ACGTCTTCAT 
CCAATCCACC TGCCCACCTC CTCTCTGACT GCACCAGTGT TGAAGCTGCA CCTCCTCAGG 
AACTGGAAGA TCCACAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029697371.1
CDS	789..7949
Translation	MSTKASQTPAEAAVVDRCSTAPSSSSKPTREVRNTNANQELQRYYDQLWNSNQRSSPPLIFYRKLPCDGEKGLIPNEALSNACLDRSSGGCEEPYQELSTGTDGIANVVPLISSEGTPCQLDSPGEGGQADRNRTGSSHCSSTMSTPQMIPNIDCCLSHTSSHLSPVHSPCFGSSPTQQKQRRGRRSSLPVSMMAFHKTSCASASEPNSSVSSPCNSPLLNHQQRHREGHFQRYRSKDGYSSLERLNRRPRVNKHSLQKLFPRRASLQTVITTGRSGLSSSPGSRDCSEDSSGSGSDGDGDGDGEELLDHTEFIRNRKERSTVLVRRFYKNNQKMTKSVCTGTRAIVRALPSGHISEEVWKSVGTYRNWRPTKKEIGPILMCGRRKKDEFRVCREMQRLVWPGIMDQMVTYYVQNRKQYPLSHPCQKLLLSCPGCVCLRVSLCPETPITDRADLSPCCRLWRCHPAHPPGVYRACLQRTSLFSLLTGALLEAIAALGARSALPYSFPQGSNSHVVLKERQLANSMTSSNALSPAISGISKELAEMRHLVQFPEEIACILTEQEQQLYQRVFPLDYLCFLTRDLGSPECQSKHHPSLKASLSVPAMSTQSSRHNAVEDLVARFNEVSSWVTWLILTAGSMEEKREVFSYLVNVAKCCWNMGNYNGVMEFLAGLRSRKVLKMWQFMDQTDIETMRSLKDAMAQHESSSEYKKVVTRALNIPGCKVVPFCGVFLKELSDALDGTASIISLKSPLENSEDSIEFVSDYSGQHNFLLRSGPDGLHIPEKEATVSNILQIIRSCNRSLEVEDTDDGSTSPSSSCPLPTSRNNSFKDRCRNHSRSFKMPQETKRALFMVGDLSDSDGDLPSEPAVKEGEFQGTEETHKAFNHGTELIPWYVLSLQPDVHQFLLQGATVIHYDQDSHLSARCLLRLQPDNTTLTWGNPQKGGASPTEPPLGLGQAVVAGLAEGLLDLGVVKAVFLGHQGVDVHAVCLQNKLSHMTVEENTLSLLYGVSTTDNRLLHFVAPNHTTQMLHKGLSELVTATRKLKKFPDQRLQWLRRQYVSLYQEDGRYEGPTLAQAIELFGGRRWNMSTGGTEKSAHQKNSPLSINDKTKKKKKVLVRGDSGDATDDEMVARKTRSCKEGTYRNGPESDSIDHEDPEDSFPGPFSLSTNKSPGLQASSSSSSSSSSMTGSNQSRPQSSPTLSGTVKAQPGAWSSRSWHGRGKGCFRGFQNLMISDSTMSFVEFVELFKSFSIRSRKDLKELFDTFAVPCIRSDPESVPLYTNLRIDDKDTGLQPDLDLLTRNGSDLGLFIRTRQQMSDNQKQISDAIAAASIVTNGTGVENASLGVLGLGIPQLNDFLVNCQREHLSYDEILSIIQKFEPSSSMRQMGWMSFEGFSRFLMDKDNFASHIEESQMNPEELQHPLSYYYIQSSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPVIYHGHTLTTKIPFKDVVEAISRSAFVNSNMPVVLSIENHCSLPQQRKMAEIFKTVFGERLVTRFLFESDFSDDPHLPSPLQLQGRILLKNKKLKAHQAPVDILKQKAHQLAHMQAQVSNGSPGVSSPGTQTNEDEEEEEDEYDYDYESLSDADVLTASSASCGLEDNLLDDKPEVKSSADKEEQPVDEIPKRMKKPDNTTQSKGKVFDMELGEEFYLPQNKKESRQIAQELSDLVIYCQAVKFPGLSTLSPAGSGRGKDRGKSRKSIFGTAPARCSATGEVTTPSRTPGKGGAERLSWEEQQTSPVLSPPTSLSAIIRTPKCYHISSVNENAAKRLCRRYSQKLIQHTVCQLLRTYPAATRIDSTNPNPLLFWLHGIQLVALNYQTDDLPMQLNTALFEANGGCGYVLKPAVLWDRNCPMYQQFCPMERDVEKMSPAVYSLAIVSGQNVCPGNSSGSPCIEVDVLGMPVDSAHFRTKPIHRNTLNPMWNEHFQFTVHFEEMCFLRVAVVENNSSQTTAQRTLPLKALKSGYRHIQLRTQHNESLEVSSLFIYSRRTEECPTGGDIPSSLLFSSEEKPASQQHRVTVYGAPGPEPFTVFSVTEQITAKQLLDMVVASAGGNPYFLCEEKVPLTKERSETKRCAQYRPLAPEEEVVRLVSSWSAEEGYVGRICFKLREEKLNEKNAAPEGEEEWSVGGAREGAGGEGAAAAEDDVFLVQIHEVSPEQPHTVIKAPRYSTAQDIIQQTLFKAKYSYSILSNPNPCDYVLMEEVTKDVGSKKSSTTKPLQRMLLDHECVYQAQSRWRGAGKFILKLKEQLVREDKKKVISFASELKKLTSRGRSMTTGGGVDGPGQRCWSAAVGLKVWVADGRVGAGRFLPSSSSGRRLHPIHLPTSSLTAPVLKLHLLRNWKIHK

Target ORF information:

RefSeq Version	XM_029841511.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029841511.1

ORF Insert Sequence:

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCATTCACG TTCATTTAAG 
ATGCCCCAAG AAACAAAACG TGCCTTGTTC ATGGTGGGAG ACTTGTCAGA TTCAGACGGA 
GACCTGCCAT CTGAGCCTGC AGTGAAAGAG GGGGAGTTTC AGGGGACTGA AGAAACACAC 
AAGGCTTTCA ACCATGGCAC CGAGCTCATT CCCTGGTACG TGTTATCGCT GCAGCCGGAT 
GTTCACCAGT TTCTGCTGCA GGGGGCCACG GTCATCCACT ACGACCAGGA CAGCCACCTC 
TCAGCACGCT GTCTGCTGCG GCTGCAGCCC GACAACACAA CGCTCACCTG GGGTAACCCA 
CAGAAAGGTG GGGCCTCCCC CACAGAGCCA CCACTGGGAT TAGGACAGGC CGTCGTCGCA 
GGACTAGCCG AGGGGCTTTT GGACCTTGGA GTGGTGAAGG CAGTGTTCCT GGGTCATCAG 
GGAGTCGATG TTCATGCTGT ATGTCTGCAA AACAAGCTGA GCCACATGAC AGTGGAGGAG 
AACACCCTGA GCCTCCTCTA TGGTGTCAGT ACCACTGACA ACAGACTTCT GCACTTTGTG 
GCTCCAAACC ACACCACGCA AATGCTCCAT AAAGGTTTGT CAGAGTTGGT GACGGCCACA 
CGAAAATTGA AGAAGTTTCC TGATCAGCGG TTGCAGTGGC TGCGGAGGCA GTATGTCAGT 
TTATATCAGG AGGATGGCAG GTATGAAGGG CCCACATTAG CCCAAGCCAT TGAGCTGTTT 
GGAGGCCGGA GATGGAACAT GAGCACAGGT GGAACAGAGA AATCTGCCCA TCAGAAAAAC 
AGTCCTTTGA GCATCAACGA TAAGACCAAG AAGAAGAAGA AGGTCCTCGT CAGGGGAGAC 
AGTGGAGATG CCACTGACGA TGAGATGGTT GCTCGGAAAA CACGGAGCTG TAAGGAAGGA 
ACATATCGAA ACGGACCGGA GTCTGACAGT ATAGATCACG AAGACCCAGA AGATAGCTTC 
CCGGGACCAT TCTCCCTCTC AACCAATAAA AGTCCTGGAT TGCAAGCCTC TTCTTCTTCC 
TCCTCCTCCT CTTCCAGCAT GACTGGCTCC AATCAGTCTC GCCCACAGTC CTCCCCAACC 
CTCTCAGGAA CAGTGAAAGC ACAGCCAGGG GCGTGGAGCA GCAGGTCATG GCATGGTCGT 
GGTAAAGGCT GCTTCAGAGG CTTCCAGAAC CTCATGATCT CTGACAGCAC AATGAGCTTT 
GTTGAGTTTG TGGAGCTTTT CAAGTCATTC AGTATCCGAA GCAGGAAGGA TCTGAAGGAA 
CTGTTTGACA CCTTTGCTGT GCCCTGCATT CGCTCAGATC CAGAGTCTGT TCCTCTTTAC 
ACCAATCTCA GGATAGATGA CAAAGACACA GGTCTACAGC CGGACCTCGA TTTATTAACG 
CGCAATGGTT CGGATCTTGG TCTGTTTATC CGAACGAGGC AGCAGATGTC TGACAACCAG 
AAGCAGATCT CAGACGCCAT TGCAGCAGCC AGCATTGTGA CCAACGGGAC GGGAGTAGAA 
AACGCATCAC TGGGGGTTTT GGGACTGGGC ATCCCTCAAC TTAACGACTT CTTGGTCAAC 
TGTCAGAGAG AACATCTGAG CTATGATGAG ATCCTCAGCA TTATACAGAA GTTTGAACCC 
TCATCAAGCA TGAGACAAAT GGGGTGGATG TCATTCGAAG GCTTTTCAAG GTTCCTGATG 
GATAAGGATA ACTTTGCCTC ACACATTGAG GAATCCCAGA TGAACCCGGA GGAACTGCAG 
CATCCCCTGT CTTATTACTA CATTCAGTCT TCTCACAATA CTTATCTGAC TGGGCACCAG 
CTTAAAGGGG AGTCCTCTGT TGAGCTTTAC AGTCAGGTGC TTTTGCAAGG CTGCAGAAGT 
GTGGAGCTGG ACTGTTGGGA TGGAGATGAT GGCATGCCGG TTATTTATCA CGGACACACT 
CTTACCACCA AGATACCATT TAAGGATGTG GTGGAAGCAA TTAGCAGGTC TGCATTTGTC 
AACTCCAACA TGCCCGTCGT CCTGTCTATA GAGAACCACT GCTCCCTCCC GCAGCAGCGC 
AAGATGGCGG AAATCTTCAA GACGGTGTTT GGGGAGCGTC TGGTGACACG CTTCCTCTTT 
GAAAGCGACT TCAGCGATGA CCCTCACCTG CCATCTCCAC TGCAGCTGCA GGGGAGGATT 
CTCCTCAAGA ATAAGAAGCT GAAAGCACAC CAGGCTCCGG TGGACATACT CAAGCAGAAG 
GCTCACCAGC TTGCCCACAT GCAGGCCCAG GTTAGCAATG GTTCACCAGG GGTTTCCTCT 
CCCGGGACCC AAACCAACGA GGACGAGGAA GAGGAAGAAG ACGAGTACGA CTATGACTAT 
GAGTCCCTCT CAGATGCTGA TGTCCTCACA GCTTCTTCTG CCTCATGTGG CCTGGAAGAC 
AACCTCCTGG ATGACAAACC TGAGGTGAAA AGCTCAGCTG ACAAAGAGGA GCAGCCTGTG 
GATGAAATAC CCAAGAGAAT GAAGAAGCCT GACAACACTA CGCAGAGTAA AGGAAAGGTG 
TTTGACATGG AACTGGGCGA GGAGTTCTAC CTTCCACAGA ACAAAAAGGA AAGTCGACAG 
ATCGCTCAGG AGCTGTCTGA CCTCGTCATC TACTGTCAGG CCGTGAAGTT CCCTGGTCTG 
TCCACGCTGT CTCCGGCTGG CTCCGGCAGA GGGAAAGACC GTGGAAAGAG CAGGAAGTCC 
ATATTTGGCA CGGCTCCAGC TCGCTGCAGC GCAACAGGGG AGGTCACCAC TCCGAGTCGC 
ACCCCTGGGA AAGGTGGCGC CGAGCGCCTG AGCTGGGAAG AGCAGCAAAC GTCTCCTGTC 
CTCAGTCCAC CCACCTCTCT CAGCGCCATT ATCCGTACAC CCAAGTGTTA TCACATCTCC 
TCCGTCAATG AGAACGCCGC CAAGCGACTG TGCCGGCGTT ACTCTCAGAA ACTGATCCAG 
CACACCGTGT GTCAGCTGCT CAGGACTTAC CCAGCAGCCA CCAGGATCGA CTCCACAAAC 
CCAAACCCTC TGCTGTTCTG GCTGCATGGC ATTCAGCTGG TGGCTCTCAA CTATCAGACT 
GACGACCTGC CCATGCAGTT AAACACAGCT CTGTTTGAGG CCAACGGCGG CTGCGGCTAT 
GTCCTGAAAC CAGCGGTGCT GTGGGACCGA AACTGTCCGA TGTATCAGCA GTTCTGTCCG 
ATGGAGAGGG ATGTGGAAAA AATGAGTCCC GCCGTCTACT CCCTTGCTAT CGTTTCGGGG 
CAGAACGTCT GTCCCGGTAA CAGCAGCGGC AGCCCCTGCA TCGAGGTGGA CGTCCTGGGG 
ATGCCGGTCG ACAGCGCCCA CTTCCGTACC AAACCCATCC ACCGCAACAC CCTCAACCCC 
ATGTGGAACG AGCACTTTCA GTTCACCGTG CACTTTGAAG AGATGTGCTT CTTGCGCGTT 
GCTGTTGTGG AGAACAACAG TTCCCAGACT ACTGCTCAGA GAACTCTACC TCTGAAAGCC 
CTCAAGTCAG GTTACAGACA CATCCAGCTG AGGACTCAAC ACAATGAATC TCTGGAAGTG 
TCAAGCTTGT TCATCTATAG CCGAAGGACA GAGGAGTGTC CGACAGGGGG CGACATTCCC 
TCATCCCTGC TGTTCAGCTC TGAGGAGAAG CCTGCATCCC AGCAGCACAG GGTGACGGTG 
TACGGAGCTC CTGGTCCTGA GCCCTTCACT GTGTTCAGTG TGACGGAGCA GATCACTGCC 
AAACAGCTGC TGGACATGGT CGTAGCTTCT GCTGGTGGAA ATCCCTACTT CCTGTGTGAG 
GAGAAGGTTC CTTTGACGAA GGAGCGCAGT GAGACAAAAC GCTGTGCTCA GTATCGCCCC 
CTTGCTCCGG AGGAGGAGGT GGTCCGACTG GTTTCCAGCT GGAGTGCCGA GGAGGGATAT 
GTGGGAAGGA TCTGCTTCAA GCTCAGAGAG GAGAAATTAA ATGAGAAGAA CGCAGCACCA 
GAAGGAGAGG AGGAGTGGAG TGTGGGCGGA GCCAGGGAGG GAGCTGGTGG AGAAGGAGCA 
GCAGCAGCAG AGGATGATGT GTTCCTGGTC CAGATCCATG AAGTTTCTCC AGAGCAGCCT 
CACACTGTGA TCAAGGCCCC GCGCTACAGC ACAGCTCAGG ACATCATCCA GCAGACGTTA 
TTCAAGGCCA AGTATTCCTA CAGCATCCTC TCCAATCCCA ATCCATGCGA CTACGTCCTA 
ATGGAGGAAG TAACCAAAGA CGTGGGCTCA AAGAAGTCCT CCACCACTAA GCCCCTGCAG 
CGGATGCTGC TGGATCACGA GTGCGTCTAT CAGGCTCAGA GCCGCTGGCG GGGCGCGGGG 
AAGTTCATTC TGAAACTGAA AGAGCAGTTG GTGCGGGAAG ACAAAAAGAA AGTCATTTCC 
TTTGCCAGTG AGCTGAAGAA ACTGACGAGC CGCGGCCGCA GCATGACGAC TGGCGGCGGC 
GTGGACGGCC CCGGCCAGAG GTGTTGGTCT GCAGCAGTCG GTCTGAAGGT GTGGGTGGCA 
GATGGAAGGG TGGGGGCAGG TAGATTCCTT CCATCTTCCT CCTCTGGGAG ACGTCTTCAT 
CCAATCCACC TGCCCACCTC CTCTCTGACT GCACCAGTGT TGAAGCTGCA CCTCCTCAGG 
AACTGGAAGA TCCACAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plce1 ( XM_029841478.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029697338.1 Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo40717
Clone ID Related Accession (Same CDS sequence)	XM_029841545.1 , XM_029841511.1 , XM_029841478.1
Accession Version	XM_029841478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCATTCACG TTCATTTAAG 
ATGCCCCAAG AAACAAAACG TGCCTTGTTC ATGGTGGGAG ACTTGTCAGA TTCAGACGGA 
GACCTGCCAT CTGAGCCTGC AGTGAAAGAG GGGGAGTTTC AGGGGACTGA AGAAACACAC 
AAGGCTTTCA ACCATGGCAC CGAGCTCATT CCCTGGTACG TGTTATCGCT GCAGCCGGAT 
GTTCACCAGT TTCTGCTGCA GGGGGCCACG GTCATCCACT ACGACCAGGA CAGCCACCTC 
TCAGCACGCT GTCTGCTGCG GCTGCAGCCC GACAACACAA CGCTCACCTG GGGTAACCCA 
CAGAAAGGTG GGGCCTCCCC CACAGAGCCA CCACTGGGAT TAGGACAGGC CGTCGTCGCA 
GGACTAGCCG AGGGGCTTTT GGACCTTGGA GTGGTGAAGG CAGTGTTCCT GGGTCATCAG 
GGAGTCGATG TTCATGCTGT ATGTCTGCAA AACAAGCTGA GCCACATGAC AGTGGAGGAG 
AACACCCTGA GCCTCCTCTA TGGTGTCAGT ACCACTGACA ACAGACTTCT GCACTTTGTG 
GCTCCAAACC ACACCACGCA AATGCTCCAT AAAGGTTTGT CAGAGTTGGT GACGGCCACA 
CGAAAATTGA AGAAGTTTCC TGATCAGCGG TTGCAGTGGC TGCGGAGGCA GTATGTCAGT 
TTATATCAGG AGGATGGCAG GTATGAAGGG CCCACATTAG CCCAAGCCAT TGAGCTGTTT 
GGAGGCCGGA GATGGAACAT GAGCACAGGT GGAACAGAGA AATCTGCCCA TCAGAAAAAC 
AGTCCTTTGA GCATCAACGA TAAGACCAAG AAGAAGAAGA AGGTCCTCGT CAGGGGAGAC 
AGTGGAGATG CCACTGACGA TGAGATGGTT GCTCGGAAAA CACGGAGCTG TAAGGAAGGA 
ACATATCGAA ACGGACCGGA GTCTGACAGT ATAGATCACG AAGACCCAGA AGATAGCTTC 
CCGGGACCAT TCTCCCTCTC AACCAATAAA AGTCCTGGAT TGCAAGCCTC TTCTTCTTCC 
TCCTCCTCCT CTTCCAGCAT GACTGGCTCC AATCAGTCTC GCCCACAGTC CTCCCCAACC 
CTCTCAGGAA CAGTGAAAGC ACAGCCAGGG GCGTGGAGCA GCAGGTCATG GCATGGTCGT 
GGTAAAGGCT GCTTCAGAGG CTTCCAGAAC CTCATGATCT CTGACAGCAC AATGAGCTTT 
GTTGAGTTTG TGGAGCTTTT CAAGTCATTC AGTATCCGAA GCAGGAAGGA TCTGAAGGAA 
CTGTTTGACA CCTTTGCTGT GCCCTGCATT CGCTCAGATC CAGAGTCTGT TCCTCTTTAC 
ACCAATCTCA GGATAGATGA CAAAGACACA GGTCTACAGC CGGACCTCGA TTTATTAACG 
CGCAATGGTT CGGATCTTGG TCTGTTTATC CGAACGAGGC AGCAGATGTC TGACAACCAG 
AAGCAGATCT CAGACGCCAT TGCAGCAGCC AGCATTGTGA CCAACGGGAC GGGAGTAGAA 
AACGCATCAC TGGGGGTTTT GGGACTGGGC ATCCCTCAAC TTAACGACTT CTTGGTCAAC 
TGTCAGAGAG AACATCTGAG CTATGATGAG ATCCTCAGCA TTATACAGAA GTTTGAACCC 
TCATCAAGCA TGAGACAAAT GGGGTGGATG TCATTCGAAG GCTTTTCAAG GTTCCTGATG 
GATAAGGATA ACTTTGCCTC ACACATTGAG GAATCCCAGA TGAACCCGGA GGAACTGCAG 
CATCCCCTGT CTTATTACTA CATTCAGTCT TCTCACAATA CTTATCTGAC TGGGCACCAG 
CTTAAAGGGG AGTCCTCTGT TGAGCTTTAC AGTCAGGTGC TTTTGCAAGG CTGCAGAAGT 
GTGGAGCTGG ACTGTTGGGA TGGAGATGAT GGCATGCCGG TTATTTATCA CGGACACACT 
CTTACCACCA AGATACCATT TAAGGATGTG GTGGAAGCAA TTAGCAGGTC TGCATTTGTC 
AACTCCAACA TGCCCGTCGT CCTGTCTATA GAGAACCACT GCTCCCTCCC GCAGCAGCGC 
AAGATGGCGG AAATCTTCAA GACGGTGTTT GGGGAGCGTC TGGTGACACG CTTCCTCTTT 
GAAAGCGACT TCAGCGATGA CCCTCACCTG CCATCTCCAC TGCAGCTGCA GGGGAGGATT 
CTCCTCAAGA ATAAGAAGCT GAAAGCACAC CAGGCTCCGG TGGACATACT CAAGCAGAAG 
GCTCACCAGC TTGCCCACAT GCAGGCCCAG GTTAGCAATG GTTCACCAGG GGTTTCCTCT 
CCCGGGACCC AAACCAACGA GGACGAGGAA GAGGAAGAAG ACGAGTACGA CTATGACTAT 
GAGTCCCTCT CAGATGCTGA TGTCCTCACA GCTTCTTCTG CCTCATGTGG CCTGGAAGAC 
AACCTCCTGG ATGACAAACC TGAGGTGAAA AGCTCAGCTG ACAAAGAGGA GCAGCCTGTG 
GATGAAATAC CCAAGAGAAT GAAGAAGCCT GACAACACTA CGCAGAGTAA AGGAAAGGTG 
TTTGACATGG AACTGGGCGA GGAGTTCTAC CTTCCACAGA ACAAAAAGGA AAGTCGACAG 
ATCGCTCAGG AGCTGTCTGA CCTCGTCATC TACTGTCAGG CCGTGAAGTT CCCTGGTCTG 
TCCACGCTGT CTCCGGCTGG CTCCGGCAGA GGGAAAGACC GTGGAAAGAG CAGGAAGTCC 
ATATTTGGCA CGGCTCCAGC TCGCTGCAGC GCAACAGGGG AGGTCACCAC TCCGAGTCGC 
ACCCCTGGGA AAGGTGGCGC CGAGCGCCTG AGCTGGGAAG AGCAGCAAAC GTCTCCTGTC 
CTCAGTCCAC CCACCTCTCT CAGCGCCATT ATCCGTACAC CCAAGTGTTA TCACATCTCC 
TCCGTCAATG AGAACGCCGC CAAGCGACTG TGCCGGCGTT ACTCTCAGAA ACTGATCCAG 
CACACCGTGT GTCAGCTGCT CAGGACTTAC CCAGCAGCCA CCAGGATCGA CTCCACAAAC 
CCAAACCCTC TGCTGTTCTG GCTGCATGGC ATTCAGCTGG TGGCTCTCAA CTATCAGACT 
GACGACCTGC CCATGCAGTT AAACACAGCT CTGTTTGAGG CCAACGGCGG CTGCGGCTAT 
GTCCTGAAAC CAGCGGTGCT GTGGGACCGA AACTGTCCGA TGTATCAGCA GTTCTGTCCG 
ATGGAGAGGG ATGTGGAAAA AATGAGTCCC GCCGTCTACT CCCTTGCTAT CGTTTCGGGG 
CAGAACGTCT GTCCCGGTAA CAGCAGCGGC AGCCCCTGCA TCGAGGTGGA CGTCCTGGGG 
ATGCCGGTCG ACAGCGCCCA CTTCCGTACC AAACCCATCC ACCGCAACAC CCTCAACCCC 
ATGTGGAACG AGCACTTTCA GTTCACCGTG CACTTTGAAG AGATGTGCTT CTTGCGCGTT 
GCTGTTGTGG AGAACAACAG TTCCCAGACT ACTGCTCAGA GAACTCTACC TCTGAAAGCC 
CTCAAGTCAG GTTACAGACA CATCCAGCTG AGGACTCAAC ACAATGAATC TCTGGAAGTG 
TCAAGCTTGT TCATCTATAG CCGAAGGACA GAGGAGTGTC CGACAGGGGG CGACATTCCC 
TCATCCCTGC TGTTCAGCTC TGAGGAGAAG CCTGCATCCC AGCAGCACAG GGTGACGGTG 
TACGGAGCTC CTGGTCCTGA GCCCTTCACT GTGTTCAGTG TGACGGAGCA GATCACTGCC 
AAACAGCTGC TGGACATGGT CGTAGCTTCT GCTGGTGGAA ATCCCTACTT CCTGTGTGAG 
GAGAAGGTTC CTTTGACGAA GGAGCGCAGT GAGACAAAAC GCTGTGCTCA GTATCGCCCC 
CTTGCTCCGG AGGAGGAGGT GGTCCGACTG GTTTCCAGCT GGAGTGCCGA GGAGGGATAT 
GTGGGAAGGA TCTGCTTCAA GCTCAGAGAG GAGAAATTAA ATGAGAAGAA CGCAGCACCA 
GAAGGAGAGG AGGAGTGGAG TGTGGGCGGA GCCAGGGAGG GAGCTGGTGG AGAAGGAGCA 
GCAGCAGCAG AGGATGATGT GTTCCTGGTC CAGATCCATG AAGTTTCTCC AGAGCAGCCT 
CACACTGTGA TCAAGGCCCC GCGCTACAGC ACAGCTCAGG ACATCATCCA GCAGACGTTA 
TTCAAGGCCA AGTATTCCTA CAGCATCCTC TCCAATCCCA ATCCATGCGA CTACGTCCTA 
ATGGAGGAAG TAACCAAAGA CGTGGGCTCA AAGAAGTCCT CCACCACTAA GCCCCTGCAG 
CGGATGCTGC TGGATCACGA GTGCGTCTAT CAGGCTCAGA GCCGCTGGCG GGGCGCGGGG 
AAGTTCATTC TGAAACTGAA AGAGCAGTTG GTGCGGGAAG ACAAAAAGAA AGTCATTTCC 
TTTGCCAGTG AGCTGAAGAA ACTGACGAGC CGCGGCCGCA GCATGACGAC TGGCGGCGGC 
GTGGACGGCC CCGGCCAGAG GTGTTGGTCT GCAGCAGTCG GTCTGAAGGT GTGGGTGGCA 
GATGGAAGGG TGGGGGCAGG TAGATTCCTT CCATCTTCCT CCTCTGGGAG ACGTCTTCAT 
CCAATCCACC TGCCCACCTC CTCTCTGACT GCACCAGTGT TGAAGCTGCA CCTCCTCAGG 
AACTGGAAGA TCCACAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029697338.1
CDS	884..8044
Translation	MSTKASQTPAEAAVVDRCSTAPSSSSKPTREVRNTNANQELQRYYDQLWNSNQRSSPPLIFYRKLPCDGEKGLIPNEALSNACLDRSSGGCEEPYQELSTGTDGIANVVPLISSEGTPCQLDSPGEGGQADRNRTGSSHCSSTMSTPQMIPNIDCCLSHTSSHLSPVHSPCFGSSPTQQKQRRGRRSSLPVSMMAFHKTSCASASEPNSSVSSPCNSPLLNHQQRHREGHFQRYRSKDGYSSLERLNRRPRVNKHSLQKLFPRRASLQTVITTGRSGLSSSPGSRDCSEDSSGSGSDGDGDGDGEELLDHTEFIRNRKERSTVLVRRFYKNNQKMTKSVCTGTRAIVRALPSGHISEEVWKSVGTYRNWRPTKKEIGPILMCGRRKKDEFRVCREMQRLVWPGIMDQMVTYYVQNRKQYPLSHPCQKLLLSCPGCVCLRVSLCPETPITDRADLSPCCRLWRCHPAHPPGVYRACLQRTSLFSLLTGALLEAIAALGARSALPYSFPQGSNSHVVLKERQLANSMTSSNALSPAISGISKELAEMRHLVQFPEEIACILTEQEQQLYQRVFPLDYLCFLTRDLGSPECQSKHHPSLKASLSVPAMSTQSSRHNAVEDLVARFNEVSSWVTWLILTAGSMEEKREVFSYLVNVAKCCWNMGNYNGVMEFLAGLRSRKVLKMWQFMDQTDIETMRSLKDAMAQHESSSEYKKVVTRALNIPGCKVVPFCGVFLKELSDALDGTASIISLKSPLENSEDSIEFVSDYSGQHNFLLRSGPDGLHIPEKEATVSNILQIIRSCNRSLEVEDTDDGSTSPSSSCPLPTSRNNSFKDRCRNHSRSFKMPQETKRALFMVGDLSDSDGDLPSEPAVKEGEFQGTEETHKAFNHGTELIPWYVLSLQPDVHQFLLQGATVIHYDQDSHLSARCLLRLQPDNTTLTWGNPQKGGASPTEPPLGLGQAVVAGLAEGLLDLGVVKAVFLGHQGVDVHAVCLQNKLSHMTVEENTLSLLYGVSTTDNRLLHFVAPNHTTQMLHKGLSELVTATRKLKKFPDQRLQWLRRQYVSLYQEDGRYEGPTLAQAIELFGGRRWNMSTGGTEKSAHQKNSPLSINDKTKKKKKVLVRGDSGDATDDEMVARKTRSCKEGTYRNGPESDSIDHEDPEDSFPGPFSLSTNKSPGLQASSSSSSSSSSMTGSNQSRPQSSPTLSGTVKAQPGAWSSRSWHGRGKGCFRGFQNLMISDSTMSFVEFVELFKSFSIRSRKDLKELFDTFAVPCIRSDPESVPLYTNLRIDDKDTGLQPDLDLLTRNGSDLGLFIRTRQQMSDNQKQISDAIAAASIVTNGTGVENASLGVLGLGIPQLNDFLVNCQREHLSYDEILSIIQKFEPSSSMRQMGWMSFEGFSRFLMDKDNFASHIEESQMNPEELQHPLSYYYIQSSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPVIYHGHTLTTKIPFKDVVEAISRSAFVNSNMPVVLSIENHCSLPQQRKMAEIFKTVFGERLVTRFLFESDFSDDPHLPSPLQLQGRILLKNKKLKAHQAPVDILKQKAHQLAHMQAQVSNGSPGVSSPGTQTNEDEEEEEDEYDYDYESLSDADVLTASSASCGLEDNLLDDKPEVKSSADKEEQPVDEIPKRMKKPDNTTQSKGKVFDMELGEEFYLPQNKKESRQIAQELSDLVIYCQAVKFPGLSTLSPAGSGRGKDRGKSRKSIFGTAPARCSATGEVTTPSRTPGKGGAERLSWEEQQTSPVLSPPTSLSAIIRTPKCYHISSVNENAAKRLCRRYSQKLIQHTVCQLLRTYPAATRIDSTNPNPLLFWLHGIQLVALNYQTDDLPMQLNTALFEANGGCGYVLKPAVLWDRNCPMYQQFCPMERDVEKMSPAVYSLAIVSGQNVCPGNSSGSPCIEVDVLGMPVDSAHFRTKPIHRNTLNPMWNEHFQFTVHFEEMCFLRVAVVENNSSQTTAQRTLPLKALKSGYRHIQLRTQHNESLEVSSLFIYSRRTEECPTGGDIPSSLLFSSEEKPASQQHRVTVYGAPGPEPFTVFSVTEQITAKQLLDMVVASAGGNPYFLCEEKVPLTKERSETKRCAQYRPLAPEEEVVRLVSSWSAEEGYVGRICFKLREEKLNEKNAAPEGEEEWSVGGAREGAGGEGAAAAEDDVFLVQIHEVSPEQPHTVIKAPRYSTAQDIIQQTLFKAKYSYSILSNPNPCDYVLMEEVTKDVGSKKSSTTKPLQRMLLDHECVYQAQSRWRGAGKFILKLKEQLVREDKKKVISFASELKKLTSRGRSMTTGGGVDGPGQRCWSAAVGLKVWVADGRVGAGRFLPSSSSGRRLHPIHLPTSSLTAPVLKLHLLRNWKIHK

Target ORF information:

RefSeq Version	XM_029841478.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029841478.1

ORF Insert Sequence:

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCATTCACG TTCATTTAAG 
ATGCCCCAAG AAACAAAACG TGCCTTGTTC ATGGTGGGAG ACTTGTCAGA TTCAGACGGA 
GACCTGCCAT CTGAGCCTGC AGTGAAAGAG GGGGAGTTTC AGGGGACTGA AGAAACACAC 
AAGGCTTTCA ACCATGGCAC CGAGCTCATT CCCTGGTACG TGTTATCGCT GCAGCCGGAT 
GTTCACCAGT TTCTGCTGCA GGGGGCCACG GTCATCCACT ACGACCAGGA CAGCCACCTC 
TCAGCACGCT GTCTGCTGCG GCTGCAGCCC GACAACACAA CGCTCACCTG GGGTAACCCA 
CAGAAAGGTG GGGCCTCCCC CACAGAGCCA CCACTGGGAT TAGGACAGGC CGTCGTCGCA 
GGACTAGCCG AGGGGCTTTT GGACCTTGGA GTGGTGAAGG CAGTGTTCCT GGGTCATCAG 
GGAGTCGATG TTCATGCTGT ATGTCTGCAA AACAAGCTGA GCCACATGAC AGTGGAGGAG 
AACACCCTGA GCCTCCTCTA TGGTGTCAGT ACCACTGACA ACAGACTTCT GCACTTTGTG 
GCTCCAAACC ACACCACGCA AATGCTCCAT AAAGGTTTGT CAGAGTTGGT GACGGCCACA 
CGAAAATTGA AGAAGTTTCC TGATCAGCGG TTGCAGTGGC TGCGGAGGCA GTATGTCAGT 
TTATATCAGG AGGATGGCAG GTATGAAGGG CCCACATTAG CCCAAGCCAT TGAGCTGTTT 
GGAGGCCGGA GATGGAACAT GAGCACAGGT GGAACAGAGA AATCTGCCCA TCAGAAAAAC 
AGTCCTTTGA GCATCAACGA TAAGACCAAG AAGAAGAAGA AGGTCCTCGT CAGGGGAGAC 
AGTGGAGATG CCACTGACGA TGAGATGGTT GCTCGGAAAA CACGGAGCTG TAAGGAAGGA 
ACATATCGAA ACGGACCGGA GTCTGACAGT ATAGATCACG AAGACCCAGA AGATAGCTTC 
CCGGGACCAT TCTCCCTCTC AACCAATAAA AGTCCTGGAT TGCAAGCCTC TTCTTCTTCC 
TCCTCCTCCT CTTCCAGCAT GACTGGCTCC AATCAGTCTC GCCCACAGTC CTCCCCAACC 
CTCTCAGGAA CAGTGAAAGC ACAGCCAGGG GCGTGGAGCA GCAGGTCATG GCATGGTCGT 
GGTAAAGGCT GCTTCAGAGG CTTCCAGAAC CTCATGATCT CTGACAGCAC AATGAGCTTT 
GTTGAGTTTG TGGAGCTTTT CAAGTCATTC AGTATCCGAA GCAGGAAGGA TCTGAAGGAA 
CTGTTTGACA CCTTTGCTGT GCCCTGCATT CGCTCAGATC CAGAGTCTGT TCCTCTTTAC 
ACCAATCTCA GGATAGATGA CAAAGACACA GGTCTACAGC CGGACCTCGA TTTATTAACG 
CGCAATGGTT CGGATCTTGG TCTGTTTATC CGAACGAGGC AGCAGATGTC TGACAACCAG 
AAGCAGATCT CAGACGCCAT TGCAGCAGCC AGCATTGTGA CCAACGGGAC GGGAGTAGAA 
AACGCATCAC TGGGGGTTTT GGGACTGGGC ATCCCTCAAC TTAACGACTT CTTGGTCAAC 
TGTCAGAGAG AACATCTGAG CTATGATGAG ATCCTCAGCA TTATACAGAA GTTTGAACCC 
TCATCAAGCA TGAGACAAAT GGGGTGGATG TCATTCGAAG GCTTTTCAAG GTTCCTGATG 
GATAAGGATA ACTTTGCCTC ACACATTGAG GAATCCCAGA TGAACCCGGA GGAACTGCAG 
CATCCCCTGT CTTATTACTA CATTCAGTCT TCTCACAATA CTTATCTGAC TGGGCACCAG 
CTTAAAGGGG AGTCCTCTGT TGAGCTTTAC AGTCAGGTGC TTTTGCAAGG CTGCAGAAGT 
GTGGAGCTGG ACTGTTGGGA TGGAGATGAT GGCATGCCGG TTATTTATCA CGGACACACT 
CTTACCACCA AGATACCATT TAAGGATGTG GTGGAAGCAA TTAGCAGGTC TGCATTTGTC 
AACTCCAACA TGCCCGTCGT CCTGTCTATA GAGAACCACT GCTCCCTCCC GCAGCAGCGC 
AAGATGGCGG AAATCTTCAA GACGGTGTTT GGGGAGCGTC TGGTGACACG CTTCCTCTTT 
GAAAGCGACT TCAGCGATGA CCCTCACCTG CCATCTCCAC TGCAGCTGCA GGGGAGGATT 
CTCCTCAAGA ATAAGAAGCT GAAAGCACAC CAGGCTCCGG TGGACATACT CAAGCAGAAG 
GCTCACCAGC TTGCCCACAT GCAGGCCCAG GTTAGCAATG GTTCACCAGG GGTTTCCTCT 
CCCGGGACCC AAACCAACGA GGACGAGGAA GAGGAAGAAG ACGAGTACGA CTATGACTAT 
GAGTCCCTCT CAGATGCTGA TGTCCTCACA GCTTCTTCTG CCTCATGTGG CCTGGAAGAC 
AACCTCCTGG ATGACAAACC TGAGGTGAAA AGCTCAGCTG ACAAAGAGGA GCAGCCTGTG 
GATGAAATAC CCAAGAGAAT GAAGAAGCCT GACAACACTA CGCAGAGTAA AGGAAAGGTG 
TTTGACATGG AACTGGGCGA GGAGTTCTAC CTTCCACAGA ACAAAAAGGA AAGTCGACAG 
ATCGCTCAGG AGCTGTCTGA CCTCGTCATC TACTGTCAGG CCGTGAAGTT CCCTGGTCTG 
TCCACGCTGT CTCCGGCTGG CTCCGGCAGA GGGAAAGACC GTGGAAAGAG CAGGAAGTCC 
ATATTTGGCA CGGCTCCAGC TCGCTGCAGC GCAACAGGGG AGGTCACCAC TCCGAGTCGC 
ACCCCTGGGA AAGGTGGCGC CGAGCGCCTG AGCTGGGAAG AGCAGCAAAC GTCTCCTGTC 
CTCAGTCCAC CCACCTCTCT CAGCGCCATT ATCCGTACAC CCAAGTGTTA TCACATCTCC 
TCCGTCAATG AGAACGCCGC CAAGCGACTG TGCCGGCGTT ACTCTCAGAA ACTGATCCAG 
CACACCGTGT GTCAGCTGCT CAGGACTTAC CCAGCAGCCA CCAGGATCGA CTCCACAAAC 
CCAAACCCTC TGCTGTTCTG GCTGCATGGC ATTCAGCTGG TGGCTCTCAA CTATCAGACT 
GACGACCTGC CCATGCAGTT AAACACAGCT CTGTTTGAGG CCAACGGCGG CTGCGGCTAT 
GTCCTGAAAC CAGCGGTGCT GTGGGACCGA AACTGTCCGA TGTATCAGCA GTTCTGTCCG 
ATGGAGAGGG ATGTGGAAAA AATGAGTCCC GCCGTCTACT CCCTTGCTAT CGTTTCGGGG 
CAGAACGTCT GTCCCGGTAA CAGCAGCGGC AGCCCCTGCA TCGAGGTGGA CGTCCTGGGG 
ATGCCGGTCG ACAGCGCCCA CTTCCGTACC AAACCCATCC ACCGCAACAC CCTCAACCCC 
ATGTGGAACG AGCACTTTCA GTTCACCGTG CACTTTGAAG AGATGTGCTT CTTGCGCGTT 
GCTGTTGTGG AGAACAACAG TTCCCAGACT ACTGCTCAGA GAACTCTACC TCTGAAAGCC 
CTCAAGTCAG GTTACAGACA CATCCAGCTG AGGACTCAAC ACAATGAATC TCTGGAAGTG 
TCAAGCTTGT TCATCTATAG CCGAAGGACA GAGGAGTGTC CGACAGGGGG CGACATTCCC 
TCATCCCTGC TGTTCAGCTC TGAGGAGAAG CCTGCATCCC AGCAGCACAG GGTGACGGTG 
TACGGAGCTC CTGGTCCTGA GCCCTTCACT GTGTTCAGTG TGACGGAGCA GATCACTGCC 
AAACAGCTGC TGGACATGGT CGTAGCTTCT GCTGGTGGAA ATCCCTACTT CCTGTGTGAG 
GAGAAGGTTC CTTTGACGAA GGAGCGCAGT GAGACAAAAC GCTGTGCTCA GTATCGCCCC 
CTTGCTCCGG AGGAGGAGGT GGTCCGACTG GTTTCCAGCT GGAGTGCCGA GGAGGGATAT 
GTGGGAAGGA TCTGCTTCAA GCTCAGAGAG GAGAAATTAA ATGAGAAGAA CGCAGCACCA 
GAAGGAGAGG AGGAGTGGAG TGTGGGCGGA GCCAGGGAGG GAGCTGGTGG AGAAGGAGCA 
GCAGCAGCAG AGGATGATGT GTTCCTGGTC CAGATCCATG AAGTTTCTCC AGAGCAGCCT 
CACACTGTGA TCAAGGCCCC GCGCTACAGC ACAGCTCAGG ACATCATCCA GCAGACGTTA 
TTCAAGGCCA AGTATTCCTA CAGCATCCTC TCCAATCCCA ATCCATGCGA CTACGTCCTA 
ATGGAGGAAG TAACCAAAGA CGTGGGCTCA AAGAAGTCCT CCACCACTAA GCCCCTGCAG 
CGGATGCTGC TGGATCACGA GTGCGTCTAT CAGGCTCAGA GCCGCTGGCG GGGCGCGGGG 
AAGTTCATTC TGAAACTGAA AGAGCAGTTG GTGCGGGAAG ACAAAAAGAA AGTCATTTCC 
TTTGCCAGTG AGCTGAAGAA ACTGACGAGC CGCGGCCGCA GCATGACGAC TGGCGGCGGC 
GTGGACGGCC CCGGCCAGAG GTGTTGGTCT GCAGCAGTCG GTCTGAAGGT GTGGGTGGCA 
GATGGAAGGG TGGGGGCAGG TAGATTCCTT CCATCTTCCT CCTCTGGGAG ACGTCTTCAT 
CCAATCCACC TGCCCACCTC CTCTCTGACT GCACCAGTGT TGAAGCTGCA CCTCCTCAGG 
AACTGGAAGA TCCACAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plce1 ( XM_029841585.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029697445.1 Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo40718
Clone ID Related Accession (Same CDS sequence)	XM_029841585.1
Accession Version	XM_029841585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCATTCACG TTCATTTAAG 
ATGCCCCAAG AAACAAAACG TGCCTTGTTC ATGGTGGGAG ACTTGTCAGA TTCAGACGGA 
GACCTGCCAT CTGAGCCTGC AGTGAAAGAG GGGGAGTTTC AGGGGACTGA AGAAACACAC 
AAGGCTTTCA ACCATGGCAC CGAGCTCATT CCCTGGTACG TGTTATCGCT GCAGCCGGAT 
GTTCACCAGT TTCTGCTGCA GGGGGCCACG GTCATCCACT ACGACCAGGA CAGCCACCTC 
TCAGCACGCT GTCTGCTGCG GCTGCAGCCC GACAACACAA CGCTCACCTG GGGTAACCCA 
CAGAAAGGTG GGGCCTCCCC CACAGAGCCA CCACTGGGAT TAGGACAGGC CGTCGTCGCA 
GGACTAGCCG AGGGGCTTTT GGACCTTGGA GTGGTGAAGG CAGTGTTCCT GGGTCATCAG 
GGAGTCGATG TTCATGCTGT ATGTCTGCAA AACAAGCTGA GCCACATGAC AGTGGAGGAG 
AACACCCTGA GCCTCCTCTA TGGTGTCAGT ACCACTGACA ACAGACTTCT GCACTTTGTG 
GCTCCAAACC ACACCACGCA AATGCTCCAT AAAGGTTTGT CAGAGTTGGT GACGGCCACA 
CGAAAATTGA AGAAGTTTCC TGATCAGCGG TTGCAGTGGC TGCGGAGGCA GTATGTCAGT 
TTATATCAGG AGGATGGCAG GTATGAAGGG CCCACATTAG CCCAAGCCAT TGAGCTGTTT 
GGAGGCCGGA GATGGAACAT GAGCACAGGT GGAACAGAGA AATCTGCCCA TCAGAAAAAC 
AGTCCTTTGA GCATCAACGA TAAGACCAAG AAGAAGAAGA AGGTCCTCGT CAGGGGAGAC 
AGTGGAGATG CCACTGACGA TGAGATGGTT GCTCGGAAAA CACGGAGCTG TAAGGAAGGA 
ACATATCGAA ACGGACCGGA GTCTGACAGT ATAGATCACG AAGACCCAGA AGATAGCTTC 
CCGGGACCAT TCTCCCTCTC AACCAATAAA AGTCCTGGAT TGCAAGCCTC TTCTTCTTCC 
TCCTCCTCCT CTTCCAGCAT GACTGGCTCC AATCAGTCTC GCCCACAGTC CTCCCCAACC 
CTCTCAGGAA CAGTGAAAGC ACAGCCAGGG GCGTGGAGCA GCAGGTCATG GCATGGTCGT 
GGTAAAGGCT GCTTCAGAGG CTTCCAGAAC CTCATGATCT CTGACAGCAC AATGAGCTTT 
GTTGAGTTTG TGGAGCTTTT CAAGTCATTC AGTATCCGAA GCAGGAAGGA TCTGAAGGAA 
CTGTTTGACA CCTTTGCTGT GCCCTGCATT CGCTCAGATC CAGAGTCTGT TCCTCTTTAC 
ACCAATCTCA GGATAGATGA CAAAGACACA GGTCTACAGC CGGACCTCGA TTTATTAACG 
CGCAATGGTT CGGATCTTGG TCTGTTTATC CGAACGAGGC AGCAGATGTC TGACAACCAG 
AAGCAGATCT CAGACGCCAT TGCAGCAGCC AGCATTGTGA CCAACGGGAC GGGAGTAGAA 
AACGCATCAC TGGGGGTTTT GGGACTGGGC ATCCCTCAAC TTAACGACTT CTTGGTCAAC 
TGTCAGAGAG AACATCTGAG CTATGATGAG ATCCTCAGCA TTATACAGAA GTTTGAACCC 
TCATCAAGCA TGAGACAAAT GGGGTGGATG TCATTCGAAG GCTTTTCAAG GTTCCTGATG 
GATAAGGATA ACTTTGCCTC ACACATTGAG GAATCCCAGA TGAACCCGGA GGAACTGCAG 
CATCCCCTGT CTTATTACTA CATTCAGTCT TCTCACAATA CTTATCTGAC TGGGCACCAG 
CTTAAAGGGG AGTCCTCTGT TGAGCTTTAC AGTCAGGTGC TTTTGCAAGG CTGCAGAAGT 
GTGGAGCTGG ACTGTTGGGA TGGAGATGAT GGCATGCCGG TTATTTATCA CGGACACACT 
CTTACCACCA AGATACCATT TAAGGATGTG GTGGAAGCAA TTAGCAGGTC TGCATTTGTC 
AACTCCAACA TGCCCGTCGT CCTGTCTATA GAGAACCACT GCTCCCTCCC GCAGCAGCGC 
AAGATGGCGG AAATCTTCAA GACGGTGTTT GGGGAGCGTC TGGTGACACG CTTCCTCTTT 
GAAAGCGACT TCAGCGATGA CCCTCACCTG CCATCTCCAC TGCAGCTGCA GGGGAGGATT 
CTCCTCAAGA ATAAGAAGCT GAAAGCACAC CAGGCTCCGG TGGACATACT CAAGCAGAAG 
GCTCACCAGC TTGCCCACAT GCAGGCCCAG GTTAGCAATG GTTCACCAGG GGTTTCCTCT 
CCCGGGACCC AAACCAACGA GGACGAGGAA GAGGAAGAAG ACGAGTACGA CTATGACTAT 
GAGTCCCTCT CAGATGACAA CCTCCTGGAT GACAAACCTG AGGTGAAAAG CTCAGCTGAC 
AAAGAGGAGC AGCCTGTGGA TGAAATACCC AAGAGAATGA AGAAGCCTGA CAACACTACG 
CAGAGTAAAG GAAAGGTGTT TGACATGGAA CTGGGCGAGG AGTTCTACCT TCCACAGAAC 
AAAAAGGAAA GTCGACAGAT CGCTCAGGAG CTGTCTGACC TCGTCATCTA CTGTCAGGCC 
GTGAAGTTCC CTGGTCTGTC CACGCTGTCT CCGGCTGGCT CCGGCAGAGG GAAAGACCGT 
GGAAAGAGCA GGAAGTCCAT ATTTGGCACG GCTCCAGCTC GCTGCAGCGC AACAGGGGAG 
GTCACCACTC CGAGTCGCAC CCCTGGGAAA GGTGGCGCCG AGCGCCTGAG CTGGGAAGAG 
CAGCAAACGT CTCCTGTCCT CAGTCCACCC ACCTCTCTCA GCGCCATTAT CCGTACACCC 
AAGTGTTATC ACATCTCCTC CGTCAATGAG AACGCCGCCA AGCGACTGTG CCGGCGTTAC 
TCTCAGAAAC TGATCCAGCA CACCGTGTGT CAGCTGCTCA GGACTTACCC AGCAGCCACC 
AGGATCGACT CCACAAACCC AAACCCTCTG CTGTTCTGGC TGCATGGCAT TCAGCTGGTG 
GCTCTCAACT ATCAGACTGA CGACCTGCCC ATGCAGTTAA ACACAGCTCT GTTTGAGGCC 
AACGGCGGCT GCGGCTATGT CCTGAAACCA GCGGTGCTGT GGGACCGAAA CTGTCCGATG 
TATCAGCAGT TCTGTCCGAT GGAGAGGGAT GTGGAAAAAA TGAGTCCCGC CGTCTACTCC 
CTTGCTATCG TTTCGGGGCA GAACGTCTGT CCCGGTAACA GCAGCGGCAG CCCCTGCATC 
GAGGTGGACG TCCTGGGGAT GCCGGTCGAC AGCGCCCACT TCCGTACCAA ACCCATCCAC 
CGCAACACCC TCAACCCCAT GTGGAACGAG CACTTTCAGT TCACCGTGCA CTTTGAAGAG 
ATGTGCTTCT TGCGCGTTGC TGTTGTGGAG AACAACAGTT CCCAGACTAC TGCTCAGAGA 
ACTCTACCTC TGAAAGCCCT CAAGTCAGGT TACAGACACA TCCAGCTGAG GACTCAACAC 
AATGAATCTC TGGAAGTGTC AAGCTTGTTC ATCTATAGCC GAAGGACAGA GGAGTGTCCG 
ACAGGGGGCG ACATTCCCTC ATCCCTGCTG TTCAGCTCTG AGGAGAAGCC TGCATCCCAG 
CAGCACAGGG TGACGGTGTA CGGAGCTCCT GGTCCTGAGC CCTTCACTGT GTTCAGTGTG 
ACGGAGCAGA TCACTGCCAA ACAGCTGCTG GACATGGTCG TAGCTTCTGC TGGTGGAAAT 
CCCTACTTCC TGTGTGAGGA GAAGGTTCCT TTGACGAAGG AGCGCAGTGA GACAAAACGC 
TGTGCTCAGT ATCGCCCCCT TGCTCCGGAG GAGGAGGTGG TCCGACTGGT TTCCAGCTGG 
AGTGCCGAGG AGGGATATGT GGGAAGGATC TGCTTCAAGC TCAGAGAGGA GAAATTAAAT 
GAGAAGAACG CAGCACCAGA AGGAGAGGAG GAGTGGAGTG TGGGCGGAGC CAGGGAGGGA 
GCTGGTGGAG AAGGAGCAGC AGCAGCAGAG GATGATGTGT TCCTGGTCCA GATCCATGAA 
GTTTCTCCAG AGCAGCCTCA CACTGTGATC AAGGCCCCGC GCTACAGCAC AGCTCAGGAC 
ATCATCCAGC AGACGTTATT CAAGGCCAAG TATTCCTACA GCATCCTCTC CAATCCCAAT 
CCATGCGACT ACGTCCTAAT GGAGGAAGTA ACCAAAGACG TGGGCTCAAA GAAGTCCTCC 
ACCACTAAGC CCCTGCAGCG GATGCTGCTG GATCACGAGT GCGTCTATCA GGCTCAGAGC 
CGCTGGCGGG GCGCGGGGAA GTTCATTCTG AAACTGAAAG AGCAGTTGGT GCGGGAAGAC 
AAAAAGAAAG TCATTTCCTT TGCCAGTGAG CTGAAGAAAC TGACGAGCCG CGGCCGCAGC 
ATGACGACTG GCGGCGGCGT GGACGGCCCC GGCCAGAGGT GTTGGTCTGC AGCAGTCGGT 
CTGAAGGTGT GGGTGGCAGA TGGAAGGGTG GGGGCAGGTA GATTCCTTCC ATCTTCCTCC 
TCTGGGAGAC GTCTTCATCC AATCCACCTG CCCACCTCCT CTCTGACTGC ACCAGTGTTG 
AAGCTGCACC TCCTCAGGAA CTGGAAGATC CACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029697445.1
CDS	694..7812
Translation	MSTKASQTPAEAAVVDRCSTAPSSSSKPTREVRNTNANQELQRYYDQLWNSNQRSSPPLIFYRKLPCDGEKGLIPNEALSNACLDRSSGGCEEPYQELSTGTDGIANVVPLISSEGTPCQLDSPGEGGQADRNRTGSSHCSSTMSTPQMIPNIDCCLSHTSSHLSPVHSPCFGSSPTQQKQRRGRRSSLPVSMMAFHKTSCASASEPNSSVSSPCNSPLLNHQQRHREGHFQRYRSKDGYSSLERLNRRPRVNKHSLQKLFPRRASLQTVITTGRSGLSSSPGSRDCSEDSSGSGSDGDGDGDGEELLDHTEFIRNRKERSTVLVRRFYKNNQKMTKSVCTGTRAIVRALPSGHISEEVWKSVGTYRNWRPTKKEIGPILMCGRRKKDEFRVCREMQRLVWPGIMDQMVTYYVQNRKQYPLSHPCQKLLLSCPGCVCLRVSLCPETPITDRADLSPCCRLWRCHPAHPPGVYRACLQRTSLFSLLTGALLEAIAALGARSALPYSFPQGSNSHVVLKERQLANSMTSSNALSPAISGISKELAEMRHLVQFPEEIACILTEQEQQLYQRVFPLDYLCFLTRDLGSPECQSKHHPSLKASLSVPAMSTQSSRHNAVEDLVARFNEVSSWVTWLILTAGSMEEKREVFSYLVNVAKCCWNMGNYNGVMEFLAGLRSRKVLKMWQFMDQTDIETMRSLKDAMAQHESSSEYKKVVTRALNIPGCKVVPFCGVFLKELSDALDGTASIISLKSPLENSEDSIEFVSDYSGQHNFLLRSGPDGLHIPEKEATVSNILQIIRSCNRSLEVEDTDDGSTSPSSSCPLPTSRNNSFKDRCRNHSRSFKMPQETKRALFMVGDLSDSDGDLPSEPAVKEGEFQGTEETHKAFNHGTELIPWYVLSLQPDVHQFLLQGATVIHYDQDSHLSARCLLRLQPDNTTLTWGNPQKGGASPTEPPLGLGQAVVAGLAEGLLDLGVVKAVFLGHQGVDVHAVCLQNKLSHMTVEENTLSLLYGVSTTDNRLLHFVAPNHTTQMLHKGLSELVTATRKLKKFPDQRLQWLRRQYVSLYQEDGRYEGPTLAQAIELFGGRRWNMSTGGTEKSAHQKNSPLSINDKTKKKKKVLVRGDSGDATDDEMVARKTRSCKEGTYRNGPESDSIDHEDPEDSFPGPFSLSTNKSPGLQASSSSSSSSSSMTGSNQSRPQSSPTLSGTVKAQPGAWSSRSWHGRGKGCFRGFQNLMISDSTMSFVEFVELFKSFSIRSRKDLKELFDTFAVPCIRSDPESVPLYTNLRIDDKDTGLQPDLDLLTRNGSDLGLFIRTRQQMSDNQKQISDAIAAASIVTNGTGVENASLGVLGLGIPQLNDFLVNCQREHLSYDEILSIIQKFEPSSSMRQMGWMSFEGFSRFLMDKDNFASHIEESQMNPEELQHPLSYYYIQSSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPVIYHGHTLTTKIPFKDVVEAISRSAFVNSNMPVVLSIENHCSLPQQRKMAEIFKTVFGERLVTRFLFESDFSDDPHLPSPLQLQGRILLKNKKLKAHQAPVDILKQKAHQLAHMQAQVSNGSPGVSSPGTQTNEDEEEEEDEYDYDYESLSDDNLLDDKPEVKSSADKEEQPVDEIPKRMKKPDNTTQSKGKVFDMELGEEFYLPQNKKESRQIAQELSDLVIYCQAVKFPGLSTLSPAGSGRGKDRGKSRKSIFGTAPARCSATGEVTTPSRTPGKGGAERLSWEEQQTSPVLSPPTSLSAIIRTPKCYHISSVNENAAKRLCRRYSQKLIQHTVCQLLRTYPAATRIDSTNPNPLLFWLHGIQLVALNYQTDDLPMQLNTALFEANGGCGYVLKPAVLWDRNCPMYQQFCPMERDVEKMSPAVYSLAIVSGQNVCPGNSSGSPCIEVDVLGMPVDSAHFRTKPIHRNTLNPMWNEHFQFTVHFEEMCFLRVAVVENNSSQTTAQRTLPLKALKSGYRHIQLRTQHNESLEVSSLFIYSRRTEECPTGGDIPSSLLFSSEEKPASQQHRVTVYGAPGPEPFTVFSVTEQITAKQLLDMVVASAGGNPYFLCEEKVPLTKERSETKRCAQYRPLAPEEEVVRLVSSWSAEEGYVGRICFKLREEKLNEKNAAPEGEEEWSVGGAREGAGGEGAAAAEDDVFLVQIHEVSPEQPHTVIKAPRYSTAQDIIQQTLFKAKYSYSILSNPNPCDYVLMEEVTKDVGSKKSSTTKPLQRMLLDHECVYQAQSRWRGAGKFILKLKEQLVREDKKKVISFASELKKLTSRGRSMTTGGGVDGPGQRCWSAAVGLKVWVADGRVGAGRFLPSSSSGRRLHPIHLPTSSLTAPVLKLHLLRNWKIHK

Target ORF information:

RefSeq Version	XM_029841585.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029841585.1

ORF Insert Sequence:

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCATTCACG TTCATTTAAG 
ATGCCCCAAG AAACAAAACG TGCCTTGTTC ATGGTGGGAG ACTTGTCAGA TTCAGACGGA 
GACCTGCCAT CTGAGCCTGC AGTGAAAGAG GGGGAGTTTC AGGGGACTGA AGAAACACAC 
AAGGCTTTCA ACCATGGCAC CGAGCTCATT CCCTGGTACG TGTTATCGCT GCAGCCGGAT 
GTTCACCAGT TTCTGCTGCA GGGGGCCACG GTCATCCACT ACGACCAGGA CAGCCACCTC 
TCAGCACGCT GTCTGCTGCG GCTGCAGCCC GACAACACAA CGCTCACCTG GGGTAACCCA 
CAGAAAGGTG GGGCCTCCCC CACAGAGCCA CCACTGGGAT TAGGACAGGC CGTCGTCGCA 
GGACTAGCCG AGGGGCTTTT GGACCTTGGA GTGGTGAAGG CAGTGTTCCT GGGTCATCAG 
GGAGTCGATG TTCATGCTGT ATGTCTGCAA AACAAGCTGA GCCACATGAC AGTGGAGGAG 
AACACCCTGA GCCTCCTCTA TGGTGTCAGT ACCACTGACA ACAGACTTCT GCACTTTGTG 
GCTCCAAACC ACACCACGCA AATGCTCCAT AAAGGTTTGT CAGAGTTGGT GACGGCCACA 
CGAAAATTGA AGAAGTTTCC TGATCAGCGG TTGCAGTGGC TGCGGAGGCA GTATGTCAGT 
TTATATCAGG AGGATGGCAG GTATGAAGGG CCCACATTAG CCCAAGCCAT TGAGCTGTTT 
GGAGGCCGGA GATGGAACAT GAGCACAGGT GGAACAGAGA AATCTGCCCA TCAGAAAAAC 
AGTCCTTTGA GCATCAACGA TAAGACCAAG AAGAAGAAGA AGGTCCTCGT CAGGGGAGAC 
AGTGGAGATG CCACTGACGA TGAGATGGTT GCTCGGAAAA CACGGAGCTG TAAGGAAGGA 
ACATATCGAA ACGGACCGGA GTCTGACAGT ATAGATCACG AAGACCCAGA AGATAGCTTC 
CCGGGACCAT TCTCCCTCTC AACCAATAAA AGTCCTGGAT TGCAAGCCTC TTCTTCTTCC 
TCCTCCTCCT CTTCCAGCAT GACTGGCTCC AATCAGTCTC GCCCACAGTC CTCCCCAACC 
CTCTCAGGAA CAGTGAAAGC ACAGCCAGGG GCGTGGAGCA GCAGGTCATG GCATGGTCGT 
GGTAAAGGCT GCTTCAGAGG CTTCCAGAAC CTCATGATCT CTGACAGCAC AATGAGCTTT 
GTTGAGTTTG TGGAGCTTTT CAAGTCATTC AGTATCCGAA GCAGGAAGGA TCTGAAGGAA 
CTGTTTGACA CCTTTGCTGT GCCCTGCATT CGCTCAGATC CAGAGTCTGT TCCTCTTTAC 
ACCAATCTCA GGATAGATGA CAAAGACACA GGTCTACAGC CGGACCTCGA TTTATTAACG 
CGCAATGGTT CGGATCTTGG TCTGTTTATC CGAACGAGGC AGCAGATGTC TGACAACCAG 
AAGCAGATCT CAGACGCCAT TGCAGCAGCC AGCATTGTGA CCAACGGGAC GGGAGTAGAA 
AACGCATCAC TGGGGGTTTT GGGACTGGGC ATCCCTCAAC TTAACGACTT CTTGGTCAAC 
TGTCAGAGAG AACATCTGAG CTATGATGAG ATCCTCAGCA TTATACAGAA GTTTGAACCC 
TCATCAAGCA TGAGACAAAT GGGGTGGATG TCATTCGAAG GCTTTTCAAG GTTCCTGATG 
GATAAGGATA ACTTTGCCTC ACACATTGAG GAATCCCAGA TGAACCCGGA GGAACTGCAG 
CATCCCCTGT CTTATTACTA CATTCAGTCT TCTCACAATA CTTATCTGAC TGGGCACCAG 
CTTAAAGGGG AGTCCTCTGT TGAGCTTTAC AGTCAGGTGC TTTTGCAAGG CTGCAGAAGT 
GTGGAGCTGG ACTGTTGGGA TGGAGATGAT GGCATGCCGG TTATTTATCA CGGACACACT 
CTTACCACCA AGATACCATT TAAGGATGTG GTGGAAGCAA TTAGCAGGTC TGCATTTGTC 
AACTCCAACA TGCCCGTCGT CCTGTCTATA GAGAACCACT GCTCCCTCCC GCAGCAGCGC 
AAGATGGCGG AAATCTTCAA GACGGTGTTT GGGGAGCGTC TGGTGACACG CTTCCTCTTT 
GAAAGCGACT TCAGCGATGA CCCTCACCTG CCATCTCCAC TGCAGCTGCA GGGGAGGATT 
CTCCTCAAGA ATAAGAAGCT GAAAGCACAC CAGGCTCCGG TGGACATACT CAAGCAGAAG 
GCTCACCAGC TTGCCCACAT GCAGGCCCAG GTTAGCAATG GTTCACCAGG GGTTTCCTCT 
CCCGGGACCC AAACCAACGA GGACGAGGAA GAGGAAGAAG ACGAGTACGA CTATGACTAT 
GAGTCCCTCT CAGATGACAA CCTCCTGGAT GACAAACCTG AGGTGAAAAG CTCAGCTGAC 
AAAGAGGAGC AGCCTGTGGA TGAAATACCC AAGAGAATGA AGAAGCCTGA CAACACTACG 
CAGAGTAAAG GAAAGGTGTT TGACATGGAA CTGGGCGAGG AGTTCTACCT TCCACAGAAC 
AAAAAGGAAA GTCGACAGAT CGCTCAGGAG CTGTCTGACC TCGTCATCTA CTGTCAGGCC 
GTGAAGTTCC CTGGTCTGTC CACGCTGTCT CCGGCTGGCT CCGGCAGAGG GAAAGACCGT 
GGAAAGAGCA GGAAGTCCAT ATTTGGCACG GCTCCAGCTC GCTGCAGCGC AACAGGGGAG 
GTCACCACTC CGAGTCGCAC CCCTGGGAAA GGTGGCGCCG AGCGCCTGAG CTGGGAAGAG 
CAGCAAACGT CTCCTGTCCT CAGTCCACCC ACCTCTCTCA GCGCCATTAT CCGTACACCC 
AAGTGTTATC ACATCTCCTC CGTCAATGAG AACGCCGCCA AGCGACTGTG CCGGCGTTAC 
TCTCAGAAAC TGATCCAGCA CACCGTGTGT CAGCTGCTCA GGACTTACCC AGCAGCCACC 
AGGATCGACT CCACAAACCC AAACCCTCTG CTGTTCTGGC TGCATGGCAT TCAGCTGGTG 
GCTCTCAACT ATCAGACTGA CGACCTGCCC ATGCAGTTAA ACACAGCTCT GTTTGAGGCC 
AACGGCGGCT GCGGCTATGT CCTGAAACCA GCGGTGCTGT GGGACCGAAA CTGTCCGATG 
TATCAGCAGT TCTGTCCGAT GGAGAGGGAT GTGGAAAAAA TGAGTCCCGC CGTCTACTCC 
CTTGCTATCG TTTCGGGGCA GAACGTCTGT CCCGGTAACA GCAGCGGCAG CCCCTGCATC 
GAGGTGGACG TCCTGGGGAT GCCGGTCGAC AGCGCCCACT TCCGTACCAA ACCCATCCAC 
CGCAACACCC TCAACCCCAT GTGGAACGAG CACTTTCAGT TCACCGTGCA CTTTGAAGAG 
ATGTGCTTCT TGCGCGTTGC TGTTGTGGAG AACAACAGTT CCCAGACTAC TGCTCAGAGA 
ACTCTACCTC TGAAAGCCCT CAAGTCAGGT TACAGACACA TCCAGCTGAG GACTCAACAC 
AATGAATCTC TGGAAGTGTC AAGCTTGTTC ATCTATAGCC GAAGGACAGA GGAGTGTCCG 
ACAGGGGGCG ACATTCCCTC ATCCCTGCTG TTCAGCTCTG AGGAGAAGCC TGCATCCCAG 
CAGCACAGGG TGACGGTGTA CGGAGCTCCT GGTCCTGAGC CCTTCACTGT GTTCAGTGTG 
ACGGAGCAGA TCACTGCCAA ACAGCTGCTG GACATGGTCG TAGCTTCTGC TGGTGGAAAT 
CCCTACTTCC TGTGTGAGGA GAAGGTTCCT TTGACGAAGG AGCGCAGTGA GACAAAACGC 
TGTGCTCAGT ATCGCCCCCT TGCTCCGGAG GAGGAGGTGG TCCGACTGGT TTCCAGCTGG 
AGTGCCGAGG AGGGATATGT GGGAAGGATC TGCTTCAAGC TCAGAGAGGA GAAATTAAAT 
GAGAAGAACG CAGCACCAGA AGGAGAGGAG GAGTGGAGTG TGGGCGGAGC CAGGGAGGGA 
GCTGGTGGAG AAGGAGCAGC AGCAGCAGAG GATGATGTGT TCCTGGTCCA GATCCATGAA 
GTTTCTCCAG AGCAGCCTCA CACTGTGATC AAGGCCCCGC GCTACAGCAC AGCTCAGGAC 
ATCATCCAGC AGACGTTATT CAAGGCCAAG TATTCCTACA GCATCCTCTC CAATCCCAAT 
CCATGCGACT ACGTCCTAAT GGAGGAAGTA ACCAAAGACG TGGGCTCAAA GAAGTCCTCC 
ACCACTAAGC CCCTGCAGCG GATGCTGCTG GATCACGAGT GCGTCTATCA GGCTCAGAGC 
CGCTGGCGGG GCGCGGGGAA GTTCATTCTG AAACTGAAAG AGCAGTTGGT GCGGGAAGAC 
AAAAAGAAAG TCATTTCCTT TGCCAGTGAG CTGAAGAAAC TGACGAGCCG CGGCCGCAGC 
ATGACGACTG GCGGCGGCGT GGACGGCCCC GGCCAGAGGT GTTGGTCTGC AGCAGTCGGT 
CTGAAGGTGT GGGTGGCAGA TGGAAGGGTG GGGGCAGGTA GATTCCTTCC ATCTTCCTCC 
TCTGGGAGAC GTCTTCATCC AATCCACCTG CCCACCTCCT CTCTGACTGC ACCAGTGTTG 
AAGCTGCACC TCCTCAGGAA CTGGAAGATC CACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plce1 ( XM_029841640.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029697500.1 Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OTo40719
Clone ID Related Accession (Same CDS sequence)	XM_029841640.1
Accession Version	XM_029841640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCAGTTCAT GGTGGGAGAC 
TTGTCAGATT CAGACGGAGA CCTGCCATCT GAGCCTGCAG TGAAAGAGGG GGAGTTTCAG 
GGGACTGAAG AAACACACAA GGCTTTCAAC CATGGCACCG AGCTCATTCC CTGGTACGTG 
TTATCGCTGC AGCCGGATGT TCACCAGTTT CTGCTGCAGG GGGCCACGGT CATCCACTAC 
GACCAGGACA GCCACCTCTC AGCACGCTGT CTGCTGCGGC TGCAGCCCGA CAACACAACG 
CTCACCTGGG GTAACCCACA GAAAGGTGGG GCCTCCCCCA CAGAGCCACC ACTGGGATTA 
GGACAGGCCG TCGTCGCAGG ACTAGCCGAG GGGCTTTTGG ACCTTGGAGT GGTGAAGGCA 
GTGTTCCTGG GTCATCAGGG AGTCGATGTT CATGCTGTAT GTCTGCAAAA CAAGCTGAGC 
CACATGACAG TGGAGGAGAA CACCCTGAGC CTCCTCTATG GTGTCAGTAC CACTGACAAC 
AGACTTCTGC ACTTTGTGGC TCCAAACCAC ACCACGCAAA TGCTCCATAA AGGTTTGTCA 
GAGTTGGTGA CGGCCACACG AAAATTGAAG AAGTTTCCTG ATCAGCGGTT GCAGTGGCTG 
CGGAGGCAGT ATGTCAGTTT ATATCAGGAG GATGGCAGGT ATGAAGGGCC CACATTAGCC 
CAAGCCATTG AGCTGTTTGG AGGCCGGAGA TGGAACATGA GCACAGGTGG AACAGAGAAA 
TCTGCCCATC AGAAAAACAG TCCTTTGAGC ATCAACGATA AGACCAAGAA GAAGAAGAAG 
GTCCTCGTCA GGGGAGACAG TGGAGATGCC ACTGACGATG AGATGGTTGC TCGGAAAACA 
CGGAGCTGTA AGGAAGGAAC ATATCGAAAC GGACCGGAGT CTGACAGTAT AGATCACGAA 
GACCCAGAAG ATAGCTTCCC GGGACCATTC TCCCTCTCAA CCAATAAAAG TCCTGGATTG 
CAAGCCTCTT CTTCTTCCTC CTCCTCCTCT TCCAGCATGA CTGGCTCCAA TCAGTCTCGC 
CCACAGTCCT CCCCAACCCT CTCAGGAACA GTGAAAGCAC AGCCAGGGGC GTGGAGCAGC 
AGGTCATGGC ATGGTCGTGG TAAAGGCTGC TTCAGAGGCT TCCAGAACCT CATGATCTCT 
GACAGCACAA TGAGCTTTGT TGAGTTTGTG GAGCTTTTCA AGTCATTCAG TATCCGAAGC 
AGGAAGGATC TGAAGGAACT GTTTGACACC TTTGCTGTGC CCTGCATTCG CTCAGATCCA 
GAGTCTGTTC CTCTTTACAC CAATCTCAGG ATAGATGACA AAGACACAGG TCTACAGCCG 
GACCTCGATT TATTAACGCG CAATGGTTCG GATCTTGGTC TGTTTATCCG AACGAGGCAG 
CAGATGTCTG ACAACCAGAA GCAGATCTCA GACGCCATTG CAGCAGCCAG CATTGTGACC 
AACGGGACGG GAGTAGAAAA CGCATCACTG GGGGTTTTGG GACTGGGCAT CCCTCAACTT 
AACGACTTCT TGGTCAACTG TCAGAGAGAA CATCTGAGCT ATGATGAGAT CCTCAGCATT 
ATACAGAAGT TTGAACCCTC ATCAAGCATG AGACAAATGG GGTGGATGTC ATTCGAAGGC 
TTTTCAAGGT TCCTGATGGA TAAGGATAAC TTTGCCTCAC ACATTGAGGA ATCCCAGATG 
AACCCGGAGG AACTGCAGCA TCCCCTGTCT TATTACTACA TTCAGTCTTC TCACAATACT 
TATCTGACTG GGCACCAGCT TAAAGGGGAG TCCTCTGTTG AGCTTTACAG TCAGGTGCTT 
TTGCAAGGCT GCAGAAGTGT GGAGCTGGAC TGTTGGGATG GAGATGATGG CATGCCGGTT 
ATTTATCACG GACACACTCT TACCACCAAG ATACCATTTA AGGATGTGGT GGAAGCAATT 
AGCAGGTCTG CATTTGTCAA CTCCAACATG CCCGTCGTCC TGTCTATAGA GAACCACTGC 
TCCCTCCCGC AGCAGCGCAA GATGGCGGAA ATCTTCAAGA CGGTGTTTGG GGAGCGTCTG 
GTGACACGCT TCCTCTTTGA AAGCGACTTC AGCGATGACC CTCACCTGCC ATCTCCACTG 
CAGCTGCAGG GGAGGATTCT CCTCAAGAAT AAGAAGCTGA AAGCACACCA GGCTCCGGTG 
GACATACTCA AGCAGAAGGC TCACCAGCTT GCCCACATGC AGGCCCAGGT TAGCAATGGT 
TCACCAGGGG TTTCCTCTCC CGGGACCCAA ACCAACGAGG ACGAGGAAGA GGAAGAAGAC 
GAGTACGACT ATGACTATGA GTCCCTCTCA GATGCTGATG TCCTCACAGC TTCTTCTGCC 
TCATGTGGCC TGGAAGACAA CCTCCTGGAT GACAAACCTG AGGTGAAAAG CTCAGCTGAC 
AAAGAGGAGC AGCCTGTGGA TGAAATACCC AAGAGAATGA AGAAGCCTGA CAACACTACG 
CAGAGTAAAG GAAAGGTGTT TGACATGGAA CTGGGCGAGG AGTTCTACCT TCCACAGAAC 
AAAAAGGAAA GTCGACAGAT CGCTCAGGAG CTGTCTGACC TCGTCATCTA CTGTCAGGCC 
GTGAAGTTCC CTGGTCTGTC CACGCTGTCT CCGGCTGGCT CCGGCAGAGG GAAAGACCGT 
GGAAAGAGCA GGAAGTCCAT ATTTGGCACG GCTCCAGCTC GCTGCAGCGC AACAGGGGAG 
GTCACCACTC CGAGTCGCAC CCCTGGGAAA GGTGGCGCCG AGCGCCTGAG CTGGGAAGAG 
CAGCAAACGT CTCCTGTCCT CAGTCCACCC ACCTCTCTCA GCGCCATTAT CCGTACACCC 
AAGTGTTATC ACATCTCCTC CGTCAATGAG AACGCCGCCA AGCGACTGTG CCGGCGTTAC 
TCTCAGAAAC TGATCCAGCA CACCGTGTGT CAGCTGCTCA GGACTTACCC AGCAGCCACC 
AGGATCGACT CCACAAACCC AAACCCTCTG CTGTTCTGGC TGCATGGCAT TCAGCTGGTG 
GCTCTCAACT ATCAGACTGA CGACCTGCCC ATGCAGTTAA ACACAGCTCT GTTTGAGGCC 
AACGGCGGCT GCGGCTATGT CCTGAAACCA GCGGTGCTGT GGGACCGAAA CTGTCCGATG 
TATCAGCAGT TCTGTCCGAT GGAGAGGGAT GTGGAAAAAA TGAGTCCCGC CGTCTACTCC 
CTTGCTATCG TTTCGGGGCA GAACGTCTGT CCCGGTAACA GCAGCGGCAG CCCCTGCATC 
GAGGTGGACG TCCTGGGGAT GCCGGTCGAC AGCGCCCACT TCCGTACCAA ACCCATCCAC 
CGCAACACCC TCAACCCCAT GTGGAACGAG CACTTTCAGT TCACCGTGCA CTTTGAAGAG 
ATGTGCTTCT TGCGCGTTGC TGTTGTGGAG AACAACAGTT CCCAGACTAC TGCTCAGAGA 
ACTCTACCTC TGAAAGCCCT CAAGTCAGGT TACAGACACA TCCAGCTGAG GACTCAACAC 
AATGAATCTC TGGAAGTGTC AAGCTTGTTC ATCTATAGCC GAAGGACAGA GGAGTGTCCG 
ACAGGGGGCG ACATTCCCTC ATCCCTGCTG TTCAGCTCTG AGGAGAAGCC TGCATCCCAG 
CAGCACAGGG TGACGGTGTA CGGAGCTCCT GGTCCTGAGC CCTTCACTGT GTTCAGTGTG 
ACGGAGCAGA TCACTGCCAA ACAGCTGCTG GACATGGTCG TAGCTTCTGC TGGTGGAAAT 
CCCTACTTCC TGTGTGAGGA GAAGGTTCCT TTGACGAAGG AGCGCAGTGA GACAAAACGC 
TGTGCTCAGT ATCGCCCCCT TGCTCCGGAG GAGGAGGTGG TCCGACTGGT TTCCAGCTGG 
AGTGCCGAGG AGGGATATGT GGGAAGGATC TGCTTCAAGC TCAGAGAGGA GAAATTAAAT 
GAGAAGAACG CAGCACCAGA AGGAGAGGAG GAGTGGAGTG TGGGCGGAGC CAGGGAGGGA 
GCTGGTGGAG AAGGAGCAGC AGCAGCAGAG GATGATGTGT TCCTGGTCCA GATCCATGAA 
GTTTCTCCAG AGCAGCCTCA CACTGTGATC AAGGCCCCGC GCTACAGCAC AGCTCAGGAC 
ATCATCCAGC AGACGTTATT CAAGGCCAAG TATTCCTACA GCATCCTCTC CAATCCCAAT 
CCATGCGACT ACGTCCTAAT GGAGGAAGTA ACCAAAGACG TGGGCTCAAA GAAGTCCTCC 
ACCACTAAGC CCCTGCAGCG GATGCTGCTG GATCACGAGT GCGTCTATCA GGCTCAGAGC 
CGCTGGCGGG GCGCGGGGAA GTTCATTCTG AAACTGAAAG AGCAGTTGGT GCGGGAAGAC 
AAAAAGAAAG TCATTTCCTT TGCCAGTGAG CTGAAGAAAC TGACGAGCCG CGGCCGCAGC 
ATGACGACTG GCGGCGGCGT GGACGGCCCC GGCCAGAGGT GTTGGTCTGC AGCAGTCGGT 
CTGAAGGTGT GGGTGGCAGA TGGAAGGGTG GGGGCAGGTA GATTCCTTCC ATCTTCCTCC 
TCTGGGAGAC GTCTTCATCC AATCCACCTG CCCACCTCCT CTCTGACTGC ACCAGTGTTG 
AAGCTGCACC TCCTCAGGAA CTGGAAGATC CACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029697500.1
CDS	696..7814
Translation	MSTKASQTPAEAAVVDRCSTAPSSSSKPTREVRNTNANQELQRYYDQLWNSNQRSSPPLIFYRKLPCDGEKGLIPNEALSNACLDRSSGGCEEPYQELSTGTDGIANVVPLISSEGTPCQLDSPGEGGQADRNRTGSSHCSSTMSTPQMIPNIDCCLSHTSSHLSPVHSPCFGSSPTQQKQRRGRRSSLPVSMMAFHKTSCASASEPNSSVSSPCNSPLLNHQQRHREGHFQRYRSKDGYSSLERLNRRPRVNKHSLQKLFPRRASLQTVITTGRSGLSSSPGSRDCSEDSSGSGSDGDGDGDGEELLDHTEFIRNRKERSTVLVRRFYKNNQKMTKSVCTGTRAIVRALPSGHISEEVWKSVGTYRNWRPTKKEIGPILMCGRRKKDEFRVCREMQRLVWPGIMDQMVTYYVQNRKQYPLSHPCQKLLLSCPGCVCLRVSLCPETPITDRADLSPCCRLWRCHPAHPPGVYRACLQRTSLFSLLTGALLEAIAALGARSALPYSFPQGSNSHVVLKERQLANSMTSSNALSPAISGISKELAEMRHLVQFPEEIACILTEQEQQLYQRVFPLDYLCFLTRDLGSPECQSKHHPSLKASLSVPAMSTQSSRHNAVEDLVARFNEVSSWVTWLILTAGSMEEKREVFSYLVNVAKCCWNMGNYNGVMEFLAGLRSRKVLKMWQFMDQTDIETMRSLKDAMAQHESSSEYKKVVTRALNIPGCKVVPFCGVFLKELSDALDGTASIISLKSPLENSEDSIEFVSDYSGQHNFLLRSGPDGLHIPEKEATVSNILQIIRSCNRSLEVEDTDDGSTSPSSSCPLPTSRNNSFKDRCRNQFMVGDLSDSDGDLPSEPAVKEGEFQGTEETHKAFNHGTELIPWYVLSLQPDVHQFLLQGATVIHYDQDSHLSARCLLRLQPDNTTLTWGNPQKGGASPTEPPLGLGQAVVAGLAEGLLDLGVVKAVFLGHQGVDVHAVCLQNKLSHMTVEENTLSLLYGVSTTDNRLLHFVAPNHTTQMLHKGLSELVTATRKLKKFPDQRLQWLRRQYVSLYQEDGRYEGPTLAQAIELFGGRRWNMSTGGTEKSAHQKNSPLSINDKTKKKKKVLVRGDSGDATDDEMVARKTRSCKEGTYRNGPESDSIDHEDPEDSFPGPFSLSTNKSPGLQASSSSSSSSSSMTGSNQSRPQSSPTLSGTVKAQPGAWSSRSWHGRGKGCFRGFQNLMISDSTMSFVEFVELFKSFSIRSRKDLKELFDTFAVPCIRSDPESVPLYTNLRIDDKDTGLQPDLDLLTRNGSDLGLFIRTRQQMSDNQKQISDAIAAASIVTNGTGVENASLGVLGLGIPQLNDFLVNCQREHLSYDEILSIIQKFEPSSSMRQMGWMSFEGFSRFLMDKDNFASHIEESQMNPEELQHPLSYYYIQSSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPVIYHGHTLTTKIPFKDVVEAISRSAFVNSNMPVVLSIENHCSLPQQRKMAEIFKTVFGERLVTRFLFESDFSDDPHLPSPLQLQGRILLKNKKLKAHQAPVDILKQKAHQLAHMQAQVSNGSPGVSSPGTQTNEDEEEEEDEYDYDYESLSDADVLTASSASCGLEDNLLDDKPEVKSSADKEEQPVDEIPKRMKKPDNTTQSKGKVFDMELGEEFYLPQNKKESRQIAQELSDLVIYCQAVKFPGLSTLSPAGSGRGKDRGKSRKSIFGTAPARCSATGEVTTPSRTPGKGGAERLSWEEQQTSPVLSPPTSLSAIIRTPKCYHISSVNENAAKRLCRRYSQKLIQHTVCQLLRTYPAATRIDSTNPNPLLFWLHGIQLVALNYQTDDLPMQLNTALFEANGGCGYVLKPAVLWDRNCPMYQQFCPMERDVEKMSPAVYSLAIVSGQNVCPGNSSGSPCIEVDVLGMPVDSAHFRTKPIHRNTLNPMWNEHFQFTVHFEEMCFLRVAVVENNSSQTTAQRTLPLKALKSGYRHIQLRTQHNESLEVSSLFIYSRRTEECPTGGDIPSSLLFSSEEKPASQQHRVTVYGAPGPEPFTVFSVTEQITAKQLLDMVVASAGGNPYFLCEEKVPLTKERSETKRCAQYRPLAPEEEVVRLVSSWSAEEGYVGRICFKLREEKLNEKNAAPEGEEEWSVGGAREGAGGEGAAAAEDDVFLVQIHEVSPEQPHTVIKAPRYSTAQDIIQQTLFKAKYSYSILSNPNPCDYVLMEEVTKDVGSKKSSTTKPLQRMLLDHECVYQAQSRWRGAGKFILKLKEQLVREDKKKVISFASELKKLTSRGRSMTTGGGVDGPGQRCWSAAVGLKVWVADGRVGAGRFLPSSSSGRRLHPIHLPTSSLTAPVLKLHLLRNWKIHK

Target ORF information:

RefSeq Version	XM_029841640.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029841640.1

ORF Insert Sequence:

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCAGTTCAT GGTGGGAGAC 
TTGTCAGATT CAGACGGAGA CCTGCCATCT GAGCCTGCAG TGAAAGAGGG GGAGTTTCAG 
GGGACTGAAG AAACACACAA GGCTTTCAAC CATGGCACCG AGCTCATTCC CTGGTACGTG 
TTATCGCTGC AGCCGGATGT TCACCAGTTT CTGCTGCAGG GGGCCACGGT CATCCACTAC 
GACCAGGACA GCCACCTCTC AGCACGCTGT CTGCTGCGGC TGCAGCCCGA CAACACAACG 
CTCACCTGGG GTAACCCACA GAAAGGTGGG GCCTCCCCCA CAGAGCCACC ACTGGGATTA 
GGACAGGCCG TCGTCGCAGG ACTAGCCGAG GGGCTTTTGG ACCTTGGAGT GGTGAAGGCA 
GTGTTCCTGG GTCATCAGGG AGTCGATGTT CATGCTGTAT GTCTGCAAAA CAAGCTGAGC 
CACATGACAG TGGAGGAGAA CACCCTGAGC CTCCTCTATG GTGTCAGTAC CACTGACAAC 
AGACTTCTGC ACTTTGTGGC TCCAAACCAC ACCACGCAAA TGCTCCATAA AGGTTTGTCA 
GAGTTGGTGA CGGCCACACG AAAATTGAAG AAGTTTCCTG ATCAGCGGTT GCAGTGGCTG 
CGGAGGCAGT ATGTCAGTTT ATATCAGGAG GATGGCAGGT ATGAAGGGCC CACATTAGCC 
CAAGCCATTG AGCTGTTTGG AGGCCGGAGA TGGAACATGA GCACAGGTGG AACAGAGAAA 
TCTGCCCATC AGAAAAACAG TCCTTTGAGC ATCAACGATA AGACCAAGAA GAAGAAGAAG 
GTCCTCGTCA GGGGAGACAG TGGAGATGCC ACTGACGATG AGATGGTTGC TCGGAAAACA 
CGGAGCTGTA AGGAAGGAAC ATATCGAAAC GGACCGGAGT CTGACAGTAT AGATCACGAA 
GACCCAGAAG ATAGCTTCCC GGGACCATTC TCCCTCTCAA CCAATAAAAG TCCTGGATTG 
CAAGCCTCTT CTTCTTCCTC CTCCTCCTCT TCCAGCATGA CTGGCTCCAA TCAGTCTCGC 
CCACAGTCCT CCCCAACCCT CTCAGGAACA GTGAAAGCAC AGCCAGGGGC GTGGAGCAGC 
AGGTCATGGC ATGGTCGTGG TAAAGGCTGC TTCAGAGGCT TCCAGAACCT CATGATCTCT 
GACAGCACAA TGAGCTTTGT TGAGTTTGTG GAGCTTTTCA AGTCATTCAG TATCCGAAGC 
AGGAAGGATC TGAAGGAACT GTTTGACACC TTTGCTGTGC CCTGCATTCG CTCAGATCCA 
GAGTCTGTTC CTCTTTACAC CAATCTCAGG ATAGATGACA AAGACACAGG TCTACAGCCG 
GACCTCGATT TATTAACGCG CAATGGTTCG GATCTTGGTC TGTTTATCCG AACGAGGCAG 
CAGATGTCTG ACAACCAGAA GCAGATCTCA GACGCCATTG CAGCAGCCAG CATTGTGACC 
AACGGGACGG GAGTAGAAAA CGCATCACTG GGGGTTTTGG GACTGGGCAT CCCTCAACTT 
AACGACTTCT TGGTCAACTG TCAGAGAGAA CATCTGAGCT ATGATGAGAT CCTCAGCATT 
ATACAGAAGT TTGAACCCTC ATCAAGCATG AGACAAATGG GGTGGATGTC ATTCGAAGGC 
TTTTCAAGGT TCCTGATGGA TAAGGATAAC TTTGCCTCAC ACATTGAGGA ATCCCAGATG 
AACCCGGAGG AACTGCAGCA TCCCCTGTCT TATTACTACA TTCAGTCTTC TCACAATACT 
TATCTGACTG GGCACCAGCT TAAAGGGGAG TCCTCTGTTG AGCTTTACAG TCAGGTGCTT 
TTGCAAGGCT GCAGAAGTGT GGAGCTGGAC TGTTGGGATG GAGATGATGG CATGCCGGTT 
ATTTATCACG GACACACTCT TACCACCAAG ATACCATTTA AGGATGTGGT GGAAGCAATT 
AGCAGGTCTG CATTTGTCAA CTCCAACATG CCCGTCGTCC TGTCTATAGA GAACCACTGC 
TCCCTCCCGC AGCAGCGCAA GATGGCGGAA ATCTTCAAGA CGGTGTTTGG GGAGCGTCTG 
GTGACACGCT TCCTCTTTGA AAGCGACTTC AGCGATGACC CTCACCTGCC ATCTCCACTG 
CAGCTGCAGG GGAGGATTCT CCTCAAGAAT AAGAAGCTGA AAGCACACCA GGCTCCGGTG 
GACATACTCA AGCAGAAGGC TCACCAGCTT GCCCACATGC AGGCCCAGGT TAGCAATGGT 
TCACCAGGGG TTTCCTCTCC CGGGACCCAA ACCAACGAGG ACGAGGAAGA GGAAGAAGAC 
GAGTACGACT ATGACTATGA GTCCCTCTCA GATGCTGATG TCCTCACAGC TTCTTCTGCC 
TCATGTGGCC TGGAAGACAA CCTCCTGGAT GACAAACCTG AGGTGAAAAG CTCAGCTGAC 
AAAGAGGAGC AGCCTGTGGA TGAAATACCC AAGAGAATGA AGAAGCCTGA CAACACTACG 
CAGAGTAAAG GAAAGGTGTT TGACATGGAA CTGGGCGAGG AGTTCTACCT TCCACAGAAC 
AAAAAGGAAA GTCGACAGAT CGCTCAGGAG CTGTCTGACC TCGTCATCTA CTGTCAGGCC 
GTGAAGTTCC CTGGTCTGTC CACGCTGTCT CCGGCTGGCT CCGGCAGAGG GAAAGACCGT 
GGAAAGAGCA GGAAGTCCAT ATTTGGCACG GCTCCAGCTC GCTGCAGCGC AACAGGGGAG 
GTCACCACTC CGAGTCGCAC CCCTGGGAAA GGTGGCGCCG AGCGCCTGAG CTGGGAAGAG 
CAGCAAACGT CTCCTGTCCT CAGTCCACCC ACCTCTCTCA GCGCCATTAT CCGTACACCC 
AAGTGTTATC ACATCTCCTC CGTCAATGAG AACGCCGCCA AGCGACTGTG CCGGCGTTAC 
TCTCAGAAAC TGATCCAGCA CACCGTGTGT CAGCTGCTCA GGACTTACCC AGCAGCCACC 
AGGATCGACT CCACAAACCC AAACCCTCTG CTGTTCTGGC TGCATGGCAT TCAGCTGGTG 
GCTCTCAACT ATCAGACTGA CGACCTGCCC ATGCAGTTAA ACACAGCTCT GTTTGAGGCC 
AACGGCGGCT GCGGCTATGT CCTGAAACCA GCGGTGCTGT GGGACCGAAA CTGTCCGATG 
TATCAGCAGT TCTGTCCGAT GGAGAGGGAT GTGGAAAAAA TGAGTCCCGC CGTCTACTCC 
CTTGCTATCG TTTCGGGGCA GAACGTCTGT CCCGGTAACA GCAGCGGCAG CCCCTGCATC 
GAGGTGGACG TCCTGGGGAT GCCGGTCGAC AGCGCCCACT TCCGTACCAA ACCCATCCAC 
CGCAACACCC TCAACCCCAT GTGGAACGAG CACTTTCAGT TCACCGTGCA CTTTGAAGAG 
ATGTGCTTCT TGCGCGTTGC TGTTGTGGAG AACAACAGTT CCCAGACTAC TGCTCAGAGA 
ACTCTACCTC TGAAAGCCCT CAAGTCAGGT TACAGACACA TCCAGCTGAG GACTCAACAC 
AATGAATCTC TGGAAGTGTC AAGCTTGTTC ATCTATAGCC GAAGGACAGA GGAGTGTCCG 
ACAGGGGGCG ACATTCCCTC ATCCCTGCTG TTCAGCTCTG AGGAGAAGCC TGCATCCCAG 
CAGCACAGGG TGACGGTGTA CGGAGCTCCT GGTCCTGAGC CCTTCACTGT GTTCAGTGTG 
ACGGAGCAGA TCACTGCCAA ACAGCTGCTG GACATGGTCG TAGCTTCTGC TGGTGGAAAT 
CCCTACTTCC TGTGTGAGGA GAAGGTTCCT TTGACGAAGG AGCGCAGTGA GACAAAACGC 
TGTGCTCAGT ATCGCCCCCT TGCTCCGGAG GAGGAGGTGG TCCGACTGGT TTCCAGCTGG 
AGTGCCGAGG AGGGATATGT GGGAAGGATC TGCTTCAAGC TCAGAGAGGA GAAATTAAAT 
GAGAAGAACG CAGCACCAGA AGGAGAGGAG GAGTGGAGTG TGGGCGGAGC CAGGGAGGGA 
GCTGGTGGAG AAGGAGCAGC AGCAGCAGAG GATGATGTGT TCCTGGTCCA GATCCATGAA 
GTTTCTCCAG AGCAGCCTCA CACTGTGATC AAGGCCCCGC GCTACAGCAC AGCTCAGGAC 
ATCATCCAGC AGACGTTATT CAAGGCCAAG TATTCCTACA GCATCCTCTC CAATCCCAAT 
CCATGCGACT ACGTCCTAAT GGAGGAAGTA ACCAAAGACG TGGGCTCAAA GAAGTCCTCC 
ACCACTAAGC CCCTGCAGCG GATGCTGCTG GATCACGAGT GCGTCTATCA GGCTCAGAGC 
CGCTGGCGGG GCGCGGGGAA GTTCATTCTG AAACTGAAAG AGCAGTTGGT GCGGGAAGAC 
AAAAAGAAAG TCATTTCCTT TGCCAGTGAG CTGAAGAAAC TGACGAGCCG CGGCCGCAGC 
ATGACGACTG GCGGCGGCGT GGACGGCCCC GGCCAGAGGT GTTGGTCTGC AGCAGTCGGT 
CTGAAGGTGT GGGTGGCAGA TGGAAGGGTG GGGGCAGGTA GATTCCTTCC ATCTTCCTCC 
TCTGGGAGAC GTCTTCATCC AATCCACCTG CCCACCTCCT CTCTGACTGC ACCAGTGTTG 
AAGCTGCACC TCCTCAGGAA CTGGAAGATC CACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plce1 ( XM_011606166.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011604468.2 Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OTo40720
Clone ID Related Accession (Same CDS sequence)	XM_011606166.2
Accession Version	XM_011606166.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011606166.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCAGTTCAT GGTGGGAGAC 
TTGTCAGATT CAGACGGAGA CCTGCCATCT GAGCCTGCAG TGAAAGAGGG GGAGTTTCAG 
GGGACTGAAG AAACACACAA GGCTTTCAAC CATGGCACCG AGCTCATTCC CTGGTACGTG 
TTATCGCTGC AGCCGGATGT TCACCAGTTT CTGCTGCAGG GGGCCACGGT CATCCACTAC 
GACCAGGACA GCCACCTCTC AGCACGCTGT CTGCTGCGGC TGCAGCCCGA CAACACAACG 
CTCACCTGGG GTAACCCACA GAAAGGTGGG GCCTCCCCCA CAGAGCCACC ACTGGGATTA 
GGACAGGCCG TCGTCGCAGG ACTAGCCGAG GGGCTTTTGG ACCTTGGAGT GGTGAAGGCA 
GTGTTCCTGG GTCATCAGGG AGTCGATGTT CATGCTGTAT GTCTGCAAAA CAAGCTGAGC 
CACATGACAG TGGAGGAGAA CACCCTGAGC CTCCTCTATG GTGTCAGTAC CACTGACAAC 
AGACTTCTGC ACTTTGTGGC TCCAAACCAC ACCACGCAAA TGCTCCATAA AGGTTTGTCA 
GAGTTGGTGA CGGCCACACG AAAATTGAAG AAGTTTCCTG ATCAGCGGTT GCAGTGGCTG 
CGGAGGCAGT ATGTCAGTTT ATATCAGGAG GATGGCAGGT ATGAAGGGCC CACATTAGCC 
CAAGCCATTG AGCTGTTTGG AGGCCGGAGA TGGAACATGA GCACAGGTGG AACAGAGAAA 
TCTGCCCATC AGAAAAACAG TCCTTTGAGC ATCAACGATA AGACCAAGAA GAAGAAGAAG 
GTCCTCGTCA GGGGAGACAG TGGAGATGCC ACTGACGATG AGATGGTTGC TCGGAAAACA 
CGGAGCTGTA AGGAAGGAAC ATATCGAAAC GGACCGGAGT CTGACAGTAT AGATCACGAA 
GACCCAGAAG ATAGCTTCCC GGGACCATTC TCCCTCTCAA CCAATAAAAG TCCTGGATTG 
CAAGCCTCTT CTTCTTCCTC CTCCTCCTCT TCCAGCATGA CTGGCTCCAA TCAGTCTCGC 
CCACAGTCCT CCCCAACCCT CTCAGGAACA GTGAAAGCAC AGCCAGGGGC GTGGAGCAGC 
AGGTCATGGC ATGGTCGTGG TAAAGGCTGC TTCAGAGGCT TCCAGAACCT CATGATCTCT 
GACAGCACAA TGAGCTTTGT TGAGTTTGTG GAGCTTTTCA AGTCATTCAG TATCCGAAGC 
AGGAAGGATC TGAAGGAACT GTTTGACACC TTTGCTGTGC CCTGCATTCG CTCAGATCCA 
GAGTCTGTTC CTCTTTACAC CAATCTCAGG ATAGATGACA AAGACACAGG TCTACAGCCG 
GACCTCGATT TATTAACGCG CAATGGTTCG GATCTTGGTC TGTTTATCCG AACGAGGCAG 
CAGATGTCTG ACAACCAGAA GCAGATCTCA GACGCCATTG CAGCAGCCAG CATTGTGACC 
AACGGGACGG GAGTAGAAAA CGCATCACTG GGGGTTTTGG GACTGGGCAT CCCTCAACTT 
AACGACTTCT TGGTCAACTG TCAGAGAGAA CATCTGAGCT ATGATGAGAT CCTCAGCATT 
ATACAGAAGT TTGAACCCTC ATCAAGCATG AGACAAATGG GGTGGATGTC ATTCGAAGGC 
TTTTCAAGGT TCCTGATGGA TAAGGATAAC TTTGCCTCAC ACATTGAGGA ATCCCAGATG 
AACCCGGAGG AACTGCAGCA TCCCCTGTCT TATTACTACA TTCAGTCTTC TCACAATACT 
TATCTGACTG GGCACCAGCT TAAAGGGGAG TCCTCTGTTG AGCTTTACAG TCAGGTGCTT 
TTGCAAGGCT GCAGAAGTGT GGAGCTGGAC TGTTGGGATG GAGATGATGG CATGCCGGTT 
ATTTATCACG GACACACTCT TACCACCAAG ATACCATTTA AGGATGTGGT GGAAGCAATT 
AGCAGGTCTG CATTTGTCAA CTCCAACATG CCCGTCGTCC TGTCTATAGA GAACCACTGC 
TCCCTCCCGC AGCAGCGCAA GATGGCGGAA ATCTTCAAGA CGGTGTTTGG GGAGCGTCTG 
GTGACACGCT TCCTCTTTGA AAGCGACTTC AGCGATGACC CTCACCTGCC ATCTCCACTG 
CAGCTGCAGG GGAGGATTCT CCTCAAGAAT AAGAAGCTGA AAGCACACCA GGCTCCGGTG 
GACATACTCA AGCAGAAGGC TCACCAGCTT GCCCACATGC AGGCCCAGGT TAGCAATGGT 
TCACCAGGGG TTTCCTCTCC CGGGACCCAA ACCAACGAGG ACGAGGAAGA GGAAGAAGAC 
GAGTACGACT ATGACTATGA GTCCCTCTCA GATGACAACC TCCTGGATGA CAAACCTGAG 
GTGAAAAGCT CAGCTGACAA AGAGGAGCAG CCTGTGGATG AAATACCCAA GAGAATGAAG 
AAGCCTGACA ACACTACGCA GAGTAAAGGA AAGGTGTTTG ACATGGAACT GGGCGAGGAG 
TTCTACCTTC CACAGAACAA AAAGGAAAGT CGACAGATCG CTCAGGAGCT GTCTGACCTC 
GTCATCTACT GTCAGGCCGT GAAGTTCCCT GGTCTGTCCA CGCTGTCTCC GGCTGGCTCC 
GGCAGAGGGA AAGACCGTGG AAAGAGCAGG AAGTCCATAT TTGGCACGGC TCCAGCTCGC 
TGCAGCGCAA CAGGGGAGGT CACCACTCCG AGTCGCACCC CTGGGAAAGG TGGCGCCGAG 
CGCCTGAGCT GGGAAGAGCA GCAAACGTCT CCTGTCCTCA GTCCACCCAC CTCTCTCAGC 
GCCATTATCC GTACACCCAA GTGTTATCAC ATCTCCTCCG TCAATGAGAA CGCCGCCAAG 
CGACTGTGCC GGCGTTACTC TCAGAAACTG ATCCAGCACA CCGTGTGTCA GCTGCTCAGG 
ACTTACCCAG CAGCCACCAG GATCGACTCC ACAAACCCAA ACCCTCTGCT GTTCTGGCTG 
CATGGCATTC AGCTGGTGGC TCTCAACTAT CAGACTGACG ACCTGCCCAT GCAGTTAAAC 
ACAGCTCTGT TTGAGGCCAA CGGCGGCTGC GGCTATGTCC TGAAACCAGC GGTGCTGTGG 
GACCGAAACT GTCCGATGTA TCAGCAGTTC TGTCCGATGG AGAGGGATGT GGAAAAAATG 
AGTCCCGCCG TCTACTCCCT TGCTATCGTT TCGGGGCAGA ACGTCTGTCC CGGTAACAGC 
AGCGGCAGCC CCTGCATCGA GGTGGACGTC CTGGGGATGC CGGTCGACAG CGCCCACTTC 
CGTACCAAAC CCATCCACCG CAACACCCTC AACCCCATGT GGAACGAGCA CTTTCAGTTC 
ACCGTGCACT TTGAAGAGAT GTGCTTCTTG CGCGTTGCTG TTGTGGAGAA CAACAGTTCC 
CAGACTACTG CTCAGAGAAC TCTACCTCTG AAAGCCCTCA AGTCAGGTTA CAGACACATC 
CAGCTGAGGA CTCAACACAA TGAATCTCTG GAAGTGTCAA GCTTGTTCAT CTATAGCCGA 
AGGACAGAGG AGTGTCCGAC AGGGGGCGAC ATTCCCTCAT CCCTGCTGTT CAGCTCTGAG 
GAGAAGCCTG CATCCCAGCA GCACAGGGTG ACGGTGTACG GAGCTCCTGG TCCTGAGCCC 
TTCACTGTGT TCAGTGTGAC GGAGCAGATC ACTGCCAAAC AGCTGCTGGA CATGGTCGTA 
GCTTCTGCTG GTGGAAATCC CTACTTCCTG TGTGAGGAGA AGGTTCCTTT GACGAAGGAG 
CGCAGTGAGA CAAAACGCTG TGCTCAGTAT CGCCCCCTTG CTCCGGAGGA GGAGGTGGTC 
CGACTGGTTT CCAGCTGGAG TGCCGAGGAG GGATATGTGG GAAGGATCTG CTTCAAGCTC 
AGAGAGGAGA AATTAAATGA GAAGAACGCA GCACCAGAAG GAGAGGAGGA GTGGAGTGTG 
GGCGGAGCCA GGGAGGGAGC TGGTGGAGAA GGAGCAGCAG CAGCAGAGGA TGATGTGTTC 
CTGGTCCAGA TCCATGAAGT TTCTCCAGAG CAGCCTCACA CTGTGATCAA GGCCCCGCGC 
TACAGCACAG CTCAGGACAT CATCCAGCAG ACGTTATTCA AGGCCAAGTA TTCCTACAGC 
ATCCTCTCCA ATCCCAATCC ATGCGACTAC GTCCTAATGG AGGAAGTAAC CAAAGACGTG 
GGCTCAAAGA AGTCCTCCAC CACTAAGCCC CTGCAGCGGA TGCTGCTGGA TCACGAGTGC 
GTCTATCAGG CTCAGAGCCG CTGGCGGGGC GCGGGGAAGT TCATTCTGAA ACTGAAAGAG 
CAGTTGGTGC GGGAAGACAA AAAGAAAGTC ATTTCCTTTG CCAGTGAGCT GAAGAAACTG 
ACGAGCCGCG GCCGCAGCAT GACGACTGGC GGCGGCGTGG ACGGCCCCGG CCAGAGGTGT 
TGGTCTGCAG CAGTCGGTCT GAAGGTGTGG GTGGCAGATG GAAGGGTGGG GGCAGGTAGA 
TTCCTTCCAT CTTCCTCCTC TGGGAGACGT CTTCATCCAA TCCACCTGCC CACCTCCTCT 
CTGACTGCAC CAGTGTTGAA GCTGCACCTC CTCAGGAACT GGAAGATCCA CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604468.2
CDS	681..7757
Translation	MSTKASQTPAEAAVVDRCSTAPSSSSKPTREVRNTNANQELQRYYDQLWNSNQRSSPPLIFYRKLPCDGEKGLIPNEALSNACLDRSSGGCEEPYQELSTGTDGIANVVPLISSEGTPCQLDSPGEGGQADRNRTGSSHCSSTMSTPQMIPNIDCCLSHTSSHLSPVHSPCFGSSPTQQKQRRGRRSSLPVSMMAFHKTSCASASEPNSSVSSPCNSPLLNHQQRHREGHFQRYRSKDGYSSLERLNRRPRVNKHSLQKLFPRRASLQTVITTGRSGLSSSPGSRDCSEDSSGSGSDGDGDGDGEELLDHTEFIRNRKERSTVLVRRFYKNNQKMTKSVCTGTRAIVRALPSGHISEEVWKSVGTYRNWRPTKKEIGPILMCGRRKKDEFRVCREMQRLVWPGIMDQMVTYYVQNRKQYPLSHPCQKLLLSCPGCVCLRVSLCPETPITDRADLSPCCRLWRCHPAHPPGVYRACLQRTSLFSLLTGALLEAIAALGARSALPYSFPQGSNSHVVLKERQLANSMTSSNALSPAISGISKELAEMRHLVQFPEEIACILTEQEQQLYQRVFPLDYLCFLTRDLGSPECQSKHHPSLKASLSVPAMSTQSSRHNAVEDLVARFNEVSSWVTWLILTAGSMEEKREVFSYLVNVAKCCWNMGNYNGVMEFLAGLRSRKVLKMWQFMDQTDIETMRSLKDAMAQHESSSEYKKVVTRALNIPGCKVVPFCGVFLKELSDALDGTASIISLKSPLENSEDSIEFVSDYSGQHNFLLRSGPDGLHIPEKEATVSNILQIIRSCNRSLEVEDTDDGSTSPSSSCPLPTSRNNSFKDRCRNQFMVGDLSDSDGDLPSEPAVKEGEFQGTEETHKAFNHGTELIPWYVLSLQPDVHQFLLQGATVIHYDQDSHLSARCLLRLQPDNTTLTWGNPQKGGASPTEPPLGLGQAVVAGLAEGLLDLGVVKAVFLGHQGVDVHAVCLQNKLSHMTVEENTLSLLYGVSTTDNRLLHFVAPNHTTQMLHKGLSELVTATRKLKKFPDQRLQWLRRQYVSLYQEDGRYEGPTLAQAIELFGGRRWNMSTGGTEKSAHQKNSPLSINDKTKKKKKVLVRGDSGDATDDEMVARKTRSCKEGTYRNGPESDSIDHEDPEDSFPGPFSLSTNKSPGLQASSSSSSSSSSMTGSNQSRPQSSPTLSGTVKAQPGAWSSRSWHGRGKGCFRGFQNLMISDSTMSFVEFVELFKSFSIRSRKDLKELFDTFAVPCIRSDPESVPLYTNLRIDDKDTGLQPDLDLLTRNGSDLGLFIRTRQQMSDNQKQISDAIAAASIVTNGTGVENASLGVLGLGIPQLNDFLVNCQREHLSYDEILSIIQKFEPSSSMRQMGWMSFEGFSRFLMDKDNFASHIEESQMNPEELQHPLSYYYIQSSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPVIYHGHTLTTKIPFKDVVEAISRSAFVNSNMPVVLSIENHCSLPQQRKMAEIFKTVFGERLVTRFLFESDFSDDPHLPSPLQLQGRILLKNKKLKAHQAPVDILKQKAHQLAHMQAQVSNGSPGVSSPGTQTNEDEEEEEDEYDYDYESLSDDNLLDDKPEVKSSADKEEQPVDEIPKRMKKPDNTTQSKGKVFDMELGEEFYLPQNKKESRQIAQELSDLVIYCQAVKFPGLSTLSPAGSGRGKDRGKSRKSIFGTAPARCSATGEVTTPSRTPGKGGAERLSWEEQQTSPVLSPPTSLSAIIRTPKCYHISSVNENAAKRLCRRYSQKLIQHTVCQLLRTYPAATRIDSTNPNPLLFWLHGIQLVALNYQTDDLPMQLNTALFEANGGCGYVLKPAVLWDRNCPMYQQFCPMERDVEKMSPAVYSLAIVSGQNVCPGNSSGSPCIEVDVLGMPVDSAHFRTKPIHRNTLNPMWNEHFQFTVHFEEMCFLRVAVVENNSSQTTAQRTLPLKALKSGYRHIQLRTQHNESLEVSSLFIYSRRTEECPTGGDIPSSLLFSSEEKPASQQHRVTVYGAPGPEPFTVFSVTEQITAKQLLDMVVASAGGNPYFLCEEKVPLTKERSETKRCAQYRPLAPEEEVVRLVSSWSAEEGYVGRICFKLREEKLNEKNAAPEGEEEWSVGGAREGAGGEGAAAAEDDVFLVQIHEVSPEQPHTVIKAPRYSTAQDIIQQTLFKAKYSYSILSNPNPCDYVLMEEVTKDVGSKKSSTTKPLQRMLLDHECVYQAQSRWRGAGKFILKLKEQLVREDKKKVISFASELKKLTSRGRSMTTGGGVDGPGQRCWSAAVGLKVWVADGRVGAGRFLPSSSSGRRLHPIHLPTSSLTAPVLKLHLLRNWKIHK

Target ORF information:

RefSeq Version	XM_011606166.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606166.2

ORF Insert Sequence:

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCAGTTCAT GGTGGGAGAC 
TTGTCAGATT CAGACGGAGA CCTGCCATCT GAGCCTGCAG TGAAAGAGGG GGAGTTTCAG 
GGGACTGAAG AAACACACAA GGCTTTCAAC CATGGCACCG AGCTCATTCC CTGGTACGTG 
TTATCGCTGC AGCCGGATGT TCACCAGTTT CTGCTGCAGG GGGCCACGGT CATCCACTAC 
GACCAGGACA GCCACCTCTC AGCACGCTGT CTGCTGCGGC TGCAGCCCGA CAACACAACG 
CTCACCTGGG GTAACCCACA GAAAGGTGGG GCCTCCCCCA CAGAGCCACC ACTGGGATTA 
GGACAGGCCG TCGTCGCAGG ACTAGCCGAG GGGCTTTTGG ACCTTGGAGT GGTGAAGGCA 
GTGTTCCTGG GTCATCAGGG AGTCGATGTT CATGCTGTAT GTCTGCAAAA CAAGCTGAGC 
CACATGACAG TGGAGGAGAA CACCCTGAGC CTCCTCTATG GTGTCAGTAC CACTGACAAC 
AGACTTCTGC ACTTTGTGGC TCCAAACCAC ACCACGCAAA TGCTCCATAA AGGTTTGTCA 
GAGTTGGTGA CGGCCACACG AAAATTGAAG AAGTTTCCTG ATCAGCGGTT GCAGTGGCTG 
CGGAGGCAGT ATGTCAGTTT ATATCAGGAG GATGGCAGGT ATGAAGGGCC CACATTAGCC 
CAAGCCATTG AGCTGTTTGG AGGCCGGAGA TGGAACATGA GCACAGGTGG AACAGAGAAA 
TCTGCCCATC AGAAAAACAG TCCTTTGAGC ATCAACGATA AGACCAAGAA GAAGAAGAAG 
GTCCTCGTCA GGGGAGACAG TGGAGATGCC ACTGACGATG AGATGGTTGC TCGGAAAACA 
CGGAGCTGTA AGGAAGGAAC ATATCGAAAC GGACCGGAGT CTGACAGTAT AGATCACGAA 
GACCCAGAAG ATAGCTTCCC GGGACCATTC TCCCTCTCAA CCAATAAAAG TCCTGGATTG 
CAAGCCTCTT CTTCTTCCTC CTCCTCCTCT TCCAGCATGA CTGGCTCCAA TCAGTCTCGC 
CCACAGTCCT CCCCAACCCT CTCAGGAACA GTGAAAGCAC AGCCAGGGGC GTGGAGCAGC 
AGGTCATGGC ATGGTCGTGG TAAAGGCTGC TTCAGAGGCT TCCAGAACCT CATGATCTCT 
GACAGCACAA TGAGCTTTGT TGAGTTTGTG GAGCTTTTCA AGTCATTCAG TATCCGAAGC 
AGGAAGGATC TGAAGGAACT GTTTGACACC TTTGCTGTGC CCTGCATTCG CTCAGATCCA 
GAGTCTGTTC CTCTTTACAC CAATCTCAGG ATAGATGACA AAGACACAGG TCTACAGCCG 
GACCTCGATT TATTAACGCG CAATGGTTCG GATCTTGGTC TGTTTATCCG AACGAGGCAG 
CAGATGTCTG ACAACCAGAA GCAGATCTCA GACGCCATTG CAGCAGCCAG CATTGTGACC 
AACGGGACGG GAGTAGAAAA CGCATCACTG GGGGTTTTGG GACTGGGCAT CCCTCAACTT 
AACGACTTCT TGGTCAACTG TCAGAGAGAA CATCTGAGCT ATGATGAGAT CCTCAGCATT 
ATACAGAAGT TTGAACCCTC ATCAAGCATG AGACAAATGG GGTGGATGTC ATTCGAAGGC 
TTTTCAAGGT TCCTGATGGA TAAGGATAAC TTTGCCTCAC ACATTGAGGA ATCCCAGATG 
AACCCGGAGG AACTGCAGCA TCCCCTGTCT TATTACTACA TTCAGTCTTC TCACAATACT 
TATCTGACTG GGCACCAGCT TAAAGGGGAG TCCTCTGTTG AGCTTTACAG TCAGGTGCTT 
TTGCAAGGCT GCAGAAGTGT GGAGCTGGAC TGTTGGGATG GAGATGATGG CATGCCGGTT 
ATTTATCACG GACACACTCT TACCACCAAG ATACCATTTA AGGATGTGGT GGAAGCAATT 
AGCAGGTCTG CATTTGTCAA CTCCAACATG CCCGTCGTCC TGTCTATAGA GAACCACTGC 
TCCCTCCCGC AGCAGCGCAA GATGGCGGAA ATCTTCAAGA CGGTGTTTGG GGAGCGTCTG 
GTGACACGCT TCCTCTTTGA AAGCGACTTC AGCGATGACC CTCACCTGCC ATCTCCACTG 
CAGCTGCAGG GGAGGATTCT CCTCAAGAAT AAGAAGCTGA AAGCACACCA GGCTCCGGTG 
GACATACTCA AGCAGAAGGC TCACCAGCTT GCCCACATGC AGGCCCAGGT TAGCAATGGT 
TCACCAGGGG TTTCCTCTCC CGGGACCCAA ACCAACGAGG ACGAGGAAGA GGAAGAAGAC 
GAGTACGACT ATGACTATGA GTCCCTCTCA GATGACAACC TCCTGGATGA CAAACCTGAG 
GTGAAAAGCT CAGCTGACAA AGAGGAGCAG CCTGTGGATG AAATACCCAA GAGAATGAAG 
AAGCCTGACA ACACTACGCA GAGTAAAGGA AAGGTGTTTG ACATGGAACT GGGCGAGGAG 
TTCTACCTTC CACAGAACAA AAAGGAAAGT CGACAGATCG CTCAGGAGCT GTCTGACCTC 
GTCATCTACT GTCAGGCCGT GAAGTTCCCT GGTCTGTCCA CGCTGTCTCC GGCTGGCTCC 
GGCAGAGGGA AAGACCGTGG AAAGAGCAGG AAGTCCATAT TTGGCACGGC TCCAGCTCGC 
TGCAGCGCAA CAGGGGAGGT CACCACTCCG AGTCGCACCC CTGGGAAAGG TGGCGCCGAG 
CGCCTGAGCT GGGAAGAGCA GCAAACGTCT CCTGTCCTCA GTCCACCCAC CTCTCTCAGC 
GCCATTATCC GTACACCCAA GTGTTATCAC ATCTCCTCCG TCAATGAGAA CGCCGCCAAG 
CGACTGTGCC GGCGTTACTC TCAGAAACTG ATCCAGCACA CCGTGTGTCA GCTGCTCAGG 
ACTTACCCAG CAGCCACCAG GATCGACTCC ACAAACCCAA ACCCTCTGCT GTTCTGGCTG 
CATGGCATTC AGCTGGTGGC TCTCAACTAT CAGACTGACG ACCTGCCCAT GCAGTTAAAC 
ACAGCTCTGT TTGAGGCCAA CGGCGGCTGC GGCTATGTCC TGAAACCAGC GGTGCTGTGG 
GACCGAAACT GTCCGATGTA TCAGCAGTTC TGTCCGATGG AGAGGGATGT GGAAAAAATG 
AGTCCCGCCG TCTACTCCCT TGCTATCGTT TCGGGGCAGA ACGTCTGTCC CGGTAACAGC 
AGCGGCAGCC CCTGCATCGA GGTGGACGTC CTGGGGATGC CGGTCGACAG CGCCCACTTC 
CGTACCAAAC CCATCCACCG CAACACCCTC AACCCCATGT GGAACGAGCA CTTTCAGTTC 
ACCGTGCACT TTGAAGAGAT GTGCTTCTTG CGCGTTGCTG TTGTGGAGAA CAACAGTTCC 
CAGACTACTG CTCAGAGAAC TCTACCTCTG AAAGCCCTCA AGTCAGGTTA CAGACACATC 
CAGCTGAGGA CTCAACACAA TGAATCTCTG GAAGTGTCAA GCTTGTTCAT CTATAGCCGA 
AGGACAGAGG AGTGTCCGAC AGGGGGCGAC ATTCCCTCAT CCCTGCTGTT CAGCTCTGAG 
GAGAAGCCTG CATCCCAGCA GCACAGGGTG ACGGTGTACG GAGCTCCTGG TCCTGAGCCC 
TTCACTGTGT TCAGTGTGAC GGAGCAGATC ACTGCCAAAC AGCTGCTGGA CATGGTCGTA 
GCTTCTGCTG GTGGAAATCC CTACTTCCTG TGTGAGGAGA AGGTTCCTTT GACGAAGGAG 
CGCAGTGAGA CAAAACGCTG TGCTCAGTAT CGCCCCCTTG CTCCGGAGGA GGAGGTGGTC 
CGACTGGTTT CCAGCTGGAG TGCCGAGGAG GGATATGTGG GAAGGATCTG CTTCAAGCTC 
AGAGAGGAGA AATTAAATGA GAAGAACGCA GCACCAGAAG GAGAGGAGGA GTGGAGTGTG 
GGCGGAGCCA GGGAGGGAGC TGGTGGAGAA GGAGCAGCAG CAGCAGAGGA TGATGTGTTC 
CTGGTCCAGA TCCATGAAGT TTCTCCAGAG CAGCCTCACA CTGTGATCAA GGCCCCGCGC 
TACAGCACAG CTCAGGACAT CATCCAGCAG ACGTTATTCA AGGCCAAGTA TTCCTACAGC 
ATCCTCTCCA ATCCCAATCC ATGCGACTAC GTCCTAATGG AGGAAGTAAC CAAAGACGTG 
GGCTCAAAGA AGTCCTCCAC CACTAAGCCC CTGCAGCGGA TGCTGCTGGA TCACGAGTGC 
GTCTATCAGG CTCAGAGCCG CTGGCGGGGC GCGGGGAAGT TCATTCTGAA ACTGAAAGAG 
CAGTTGGTGC GGGAAGACAA AAAGAAAGTC ATTTCCTTTG CCAGTGAGCT GAAGAAACTG 
ACGAGCCGCG GCCGCAGCAT GACGACTGGC GGCGGCGTGG ACGGCCCCGG CCAGAGGTGT 
TGGTCTGCAG CAGTCGGTCT GAAGGTGTGG GTGGCAGATG GAAGGGTGGG GGCAGGTAGA 
TTCCTTCCAT CTTCCTCCTC TGGGAGACGT CTTCATCCAA TCCACCTGCC CACCTCCTCT 
CTGACTGCAC CAGTGTTGAA GCTGCACCTC CTCAGGAACT GGAAGATCCA CAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plce1 ( XM_029841739.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029697599.1 Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OTo40721
Clone ID Related Accession (Same CDS sequence)	XM_029841739.1
Accession Version	XM_029841739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCATTCACG TTCATTTAAG 
ATGCCCCAAG AAACAAAACG TGCCTTGTTC ATGGTGGGAG ACTTGTCAGA TTCAGACGGA 
GACCTGCCAT CTGAGCCTGC AGTGAAAGAG GGGGAGTTTC AGGGGACTGA AGAAACACAC 
AAGGCTTTCA ACCATGGCAC CGAGCTCATT CCCTGGTACG TGTTATCGCT GCAGCCGGAT 
GTTCACCAGT TTCTGCTGCA GGGGGCCACG GTCATCCACT ACGACCAGGA CAGCCACCTC 
TCAGCACGCT GTCTGCTGCG GCTGCAGCCC GACAACACAA CGCTCACCTG GGGTAACCCA 
CAGAAAGGTG GGGCCTCCCC CACAGAGCCA CCACTGGGAT TAGGACAGGC CGTCGTCGCA 
GGACTAGCCG AGGGGCTTTT GGACCTTGGA GTGGTGAAGG CAGTGTTCCT GGGTCATCAG 
GGAGTCGATG TTCATGCTGT ATGTCTGCAA AACAAGCTGA GCCACATGAC AGTGGAGGAG 
AACACCCTGA GCCTCCTCTA TGGTGTCAGT ACCACTGACA ACAGACTTCT GCACTTTGTG 
GCTCCAAACC ACACCACGCA AATGCTCCAT AAAGGTTTGT CAGAGTTGGT GACGGCCACA 
CGAAAATTGA AGAAGTTTCC TGATCAGCGG TTGCAGTGGC TGCGGAGGCA GTATGTCAGT 
TTATATCAGG AGGATGGCAG GTATGAAGGG CCCACATTAG CCCAAGCCAT TGAGCTGTTT 
GGAGGCCGGA GATGGAACAT GAGCACAGGT GGAACAGAGA AATCTGCCCA TCAGAAAAAC 
AGTCCTTTGA GCATCAACGA TAAGACCAAG AAGAAGAAGA AGGTCCTCGT CAGGGGAGAC 
AGTGGAGATG CCACTGACGA TGAGATGGTT GCTCGGAAAA CACGGAGCTG TAAGGAAGGA 
ACATATCGAA ACGGACCGGA GTCTGACAGT ATAGATCACG AAGACCCAGA AGATAGCTTC 
CCGGGACCAT TCTCCCTCTC AACCAATAAA AGTCCTGGAT TGCAAGCCTC TTCTTCTTCC 
TCCTCCTCCT CTTCCAGCAT GACTGGCTCC AATCAGTCTC GCCCACAGTC CTCCCCAACC 
CTCTCAGGAA CAGTGAAAGC ACAGCCAGGG GCGTGGAGCA GCAGGTCATG GCATGGTCGT 
GGTAAAGGCT GCTTCAGAGG CTTCCAGAAC CTCATGATCT CTGACAGCAC AATGAGCTTT 
GTTGAGTTTG TGGAGCTTTT CAAGTCATTC AGTATCCGAA GCAGGAAGGA TCTGAAGGAA 
CTGTTTGACA CCTTTGCTGT GCCCTGCATT CGCTCAGATC CAGAGTCTGT TCCTCTTTAC 
ACCAATCTCA GGATAGATGA CAAAGACACA GGTCTACAGC CGGACCTCGA TTTATTAACG 
CGCAATGGTT CGGATCTTGG TCTGTTTATC CGAACGAGGC AGCAGATGTC TGACAACCAG 
AAGCAGATCT CAGACGCCAT TGCAGCAGCC AGCATTGTGA CCAACGGGAC GGGAGTAGAA 
AACGCATCAC TGGGGGTTTT GGGACTGGGC ATCCCTCAAC TTAACGACTT CTTGGTCAAC 
TGTCAGAGAG AACATCTGAG CTATGATGAG ATCCTCAGCA TTATACAGAA GTTTGAACCC 
TCATCAAGCA TGAGACAAAT GGGGTGGATG TCATTCGAAG GCTTTTCAAG GTTCCTGATG 
GATAAGGATA ACTTTGCCTC ACACATTGAG GAATCCCAGA TGAACCCGGA GGAACTGCAG 
CATCCCCTGT CTTATTACTA CATTCAGTCT TCTCACAATA CTTATCTGAC TGGGCACCAG 
CTTAAAGGGG AGTCCTCTGT TGAGCTTTAC AGTCAGGTGC TTTTGCAAGG CTGCAGAAGT 
GTGGAGCTGG ACTGTTGGGA TGGAGATGAT GGCATGCCGG TTATTTATCA CGGACACACT 
CTTACCACCA AGATACCATT TAAGGATGTG GTGGAAGCAA TTAGCAGGTC TGCATTTGTC 
AACTCCAACA TGCCCGTCGT CCTGTCTATA GAGAACCACT GCTCCCTCCC GCAGCAGCGC 
AAGATGGCGG AAATCTTCAA GACGGTGTTT GGGGAGCGTC TGGTGACACG CTTCCTCTTT 
GAAAGCGACT TCAGCGATGA CCCTCACCTG CCATCTCCAC TGCAGCTGCA GGGGAGGATT 
CTCCTCAAGA ATAAGAAGCT GAAAGCACAC CAGGCTCCGG TGGACATACT CAAGCAGAAG 
GCTCACCAGC TTGCCCACAT GCAGGCCCAG GTTAGCAATG GTTCACCAGG GGTTTCCTCT 
CCCGGGACCC AAACCAACGA GGACGAGGAA GAGGAAGAAG ACGAGTACGA CTATGACTAT 
GAGTCCCTCT CAGATGCTGA TGTCCTCACA GCTTCTTCTG CCTCATGTGG CCTGGAAGAC 
AACCTCCTGG ATGACAAACC TGAGGTGAAA AGCTCAGCTG ACAAAGAGGA GCAGCCTGTG 
GATGAAATAC CCAAGAGAAT GAAGAAGCCT GACAACACTA CGCAGAGTAA AGGAAAGGTG 
TTTGACATGG AACTGGGCGA GGAGTTCTAC CTTCCACAGA ACAAAAAGGA AAGTCGACAG 
ATCGCTCAGG AGCTGTCTGA CCTCGTCATC TACTGTCAGG CCGTGAAGTT CCCTGGTCTG 
TCCACGCTGT CTCCGGCTGG CTCCGGCAGA GGGAAAGACC GTGGAAAGAG CAGGAAGTCC 
ATATTTGGCA CGGCTCCAGC TCGCTGCAGC GCAACAGGGG AGGTCACCAC TCCGAGTCGC 
ACCCCTGGGA AAGGTGGCGC CGAGCGCCTG AGCTGGGAAG AGCAGCAAAC GTCTCCTGTC 
CTCAGTCCAC CCACCTCTCT CAGCGCCATT ATCCGTACAC CCAAGTGTTA TCACATCTCC 
TCCGTCAATG AGAACGCCGC CAAGCGACTG TGCCGGCGTT ACTCTCAGAA ACTGATCCAG 
CACACCGTGT GTCAGCTGCT CAGGACTTAC CCAGCAGCCA CCAGGATCGA CTCCACAAAC 
CCAAACCCTC TGCTGTTCTG GCTGCATGGC ATTCAGCTGG TGGCTCTCAA CTATCAGACT 
GACGACCTGC CCATGCAGTT AAACACAGCT CTGTTTGAGG CCAACGGCGG CTGCGGCTAT 
GTCCTGAAAC CAGCGGTGCT GTGGGACCGA AACTGTCCGA TGTATCAGCA GTTCTGTCCG 
ATGGAGAGGG ATGTGGAAAA AATGAGTCCC GCCGTCTACT CCCTTGCTAT CGTTTCGGGG 
CAGAACGTCT GTCCCGGTAA CAGCAGCGGC AGCCCCTGCA TCGAGGTGGA CGTCCTGGGG 
ATGCCGGTCG ACAGCGCCCA CTTCCGTACC AAACCCATCC ACCGCAACAC CCTCAACCCC 
ATGTGGAACG AGCACTTTCA GTTCACCGTG CACTTTGAAG AGATGTGCTT CTTGCGCGTT 
GCTGTTGTGG AGAACAACAG TTCCCAGACT ACTGCTCAGA GAACTCTACC TCTGAAAGCC 
CTCAAGTCAG GTTACAGACA CATCCAGCTG AGGACTCAAC ACAATGAATC TCTGGAAGTG 
TCAAGCTTGT TCATCTATAG CCGAAGGACA GAGGAGTGTC CGACAGGGGG CGACATTCCC 
TCATCCCTGC TGTTCAGCTC TGAGGAGAAG CCTGCATCCC AGCAGCACAG GGTGACGGTG 
TACGGAGCTC CTGGTCCTGA GCCCTTCACT GTGTTCAGTG TGACGGAGCA GATCACTGCC 
AAACAGCTGC TGGACATGGT CGTAGCTTCT GCTGGTGGAA ATCCCTACTT CCTGTGTGAG 
GAGAAGGTTC CTTTGACGAA GGAGCGCAGT GAGACAAAAC GCTGTGCTCA GTATCGCCCC 
CTTGCTCCGG AGGAGGAGGT GGTCCGACTG GTTTCCAGCT GGAGTGCCGA GGAGGGATAT 
GTGGGAAGGA TCTGCTTCAA GCTCAGAGAG GAGAAATTAA ATGAGAAGAA CGCAGCACCA 
GAAGGAGAGG AGGAGTGGAG TGTGGGCGGA GCCAGGGAGG GAGCTGGTGG AGAAGGAGCA 
GCAGCAGCAG AGGATGATGT GTTCCTGGTC CAGATCCATG AAGTTTCTCC AGAGCAGCCT 
CACACTGTGA TCAAGGCCCC GCGCTACAGC ACAGCTCAGG ACATCATCCA GCAGACGTTA 
TTCAAGGCCA AGTATTCCTA CAGCATCCTC TCCAATCCCA ATCCATGCGA CTACGTCCTA 
ATGGAGGAAG TAACCAAAGA CGTGGGCTCA AAGAAGTCCT CCACCACTAA GCCCCTGCAG 
CGGATGCTGC TGGATCACGA GTGCGTCTAT CAGGCTCAGA GCCGCTGGCG GGGCGCGGGG 
AAGTTCATTC TGAAACTGAA AGAGCAGTTG GTGCGGGAAG ACAAAAAGAA AGTCATTTCC 
TTTGCCAGTG AGCTGAAGAA ACTGACGAGC CGCGGCCGCA GCATGACGAC TGGCGGCGGC 
GTGGACGGCC CCGGCCAGAG TAAGGATGAT AGAGCTGCAT GTTGTGTGGC TCTGACAGAG 
CTCCAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029697599.1
CDS	692..7723
Translation	MSTKASQTPAEAAVVDRCSTAPSSSSKPTREVRNTNANQELQRYYDQLWNSNQRSSPPLIFYRKLPCDGEKGLIPNEALSNACLDRSSGGCEEPYQELSTGTDGIANVVPLISSEGTPCQLDSPGEGGQADRNRTGSSHCSSTMSTPQMIPNIDCCLSHTSSHLSPVHSPCFGSSPTQQKQRRGRRSSLPVSMMAFHKTSCASASEPNSSVSSPCNSPLLNHQQRHREGHFQRYRSKDGYSSLERLNRRPRVNKHSLQKLFPRRASLQTVITTGRSGLSSSPGSRDCSEDSSGSGSDGDGDGDGEELLDHTEFIRNRKERSTVLVRRFYKNNQKMTKSVCTGTRAIVRALPSGHISEEVWKSVGTYRNWRPTKKEIGPILMCGRRKKDEFRVCREMQRLVWPGIMDQMVTYYVQNRKQYPLSHPCQKLLLSCPGCVCLRVSLCPETPITDRADLSPCCRLWRCHPAHPPGVYRACLQRTSLFSLLTGALLEAIAALGARSALPYSFPQGSNSHVVLKERQLANSMTSSNALSPAISGISKELAEMRHLVQFPEEIACILTEQEQQLYQRVFPLDYLCFLTRDLGSPECQSKHHPSLKASLSVPAMSTQSSRHNAVEDLVARFNEVSSWVTWLILTAGSMEEKREVFSYLVNVAKCCWNMGNYNGVMEFLAGLRSRKVLKMWQFMDQTDIETMRSLKDAMAQHESSSEYKKVVTRALNIPGCKVVPFCGVFLKELSDALDGTASIISLKSPLENSEDSIEFVSDYSGQHNFLLRSGPDGLHIPEKEATVSNILQIIRSCNRSLEVEDTDDGSTSPSSSCPLPTSRNNSFKDRCRNHSRSFKMPQETKRALFMVGDLSDSDGDLPSEPAVKEGEFQGTEETHKAFNHGTELIPWYVLSLQPDVHQFLLQGATVIHYDQDSHLSARCLLRLQPDNTTLTWGNPQKGGASPTEPPLGLGQAVVAGLAEGLLDLGVVKAVFLGHQGVDVHAVCLQNKLSHMTVEENTLSLLYGVSTTDNRLLHFVAPNHTTQMLHKGLSELVTATRKLKKFPDQRLQWLRRQYVSLYQEDGRYEGPTLAQAIELFGGRRWNMSTGGTEKSAHQKNSPLSINDKTKKKKKVLVRGDSGDATDDEMVARKTRSCKEGTYRNGPESDSIDHEDPEDSFPGPFSLSTNKSPGLQASSSSSSSSSSMTGSNQSRPQSSPTLSGTVKAQPGAWSSRSWHGRGKGCFRGFQNLMISDSTMSFVEFVELFKSFSIRSRKDLKELFDTFAVPCIRSDPESVPLYTNLRIDDKDTGLQPDLDLLTRNGSDLGLFIRTRQQMSDNQKQISDAIAAASIVTNGTGVENASLGVLGLGIPQLNDFLVNCQREHLSYDEILSIIQKFEPSSSMRQMGWMSFEGFSRFLMDKDNFASHIEESQMNPEELQHPLSYYYIQSSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPVIYHGHTLTTKIPFKDVVEAISRSAFVNSNMPVVLSIENHCSLPQQRKMAEIFKTVFGERLVTRFLFESDFSDDPHLPSPLQLQGRILLKNKKLKAHQAPVDILKQKAHQLAHMQAQVSNGSPGVSSPGTQTNEDEEEEEDEYDYDYESLSDADVLTASSASCGLEDNLLDDKPEVKSSADKEEQPVDEIPKRMKKPDNTTQSKGKVFDMELGEEFYLPQNKKESRQIAQELSDLVIYCQAVKFPGLSTLSPAGSGRGKDRGKSRKSIFGTAPARCSATGEVTTPSRTPGKGGAERLSWEEQQTSPVLSPPTSLSAIIRTPKCYHISSVNENAAKRLCRRYSQKLIQHTVCQLLRTYPAATRIDSTNPNPLLFWLHGIQLVALNYQTDDLPMQLNTALFEANGGCGYVLKPAVLWDRNCPMYQQFCPMERDVEKMSPAVYSLAIVSGQNVCPGNSSGSPCIEVDVLGMPVDSAHFRTKPIHRNTLNPMWNEHFQFTVHFEEMCFLRVAVVENNSSQTTAQRTLPLKALKSGYRHIQLRTQHNESLEVSSLFIYSRRTEECPTGGDIPSSLLFSSEEKPASQQHRVTVYGAPGPEPFTVFSVTEQITAKQLLDMVVASAGGNPYFLCEEKVPLTKERSETKRCAQYRPLAPEEEVVRLVSSWSAEEGYVGRICFKLREEKLNEKNAAPEGEEEWSVGGAREGAGGEGAAAAEDDVFLVQIHEVSPEQPHTVIKAPRYSTAQDIIQQTLFKAKYSYSILSNPNPCDYVLMEEVTKDVGSKKSSTTKPLQRMLLDHECVYQAQSRWRGAGKFILKLKEQLVREDKKKVISFASELKKLTSRGRSMTTGGGVDGPGQSKDDRAACCVALTELQE

Target ORF information:

RefSeq Version	XM_029841739.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029841739.1

ORF Insert Sequence:

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCATTCACG TTCATTTAAG 
ATGCCCCAAG AAACAAAACG TGCCTTGTTC ATGGTGGGAG ACTTGTCAGA TTCAGACGGA 
GACCTGCCAT CTGAGCCTGC AGTGAAAGAG GGGGAGTTTC AGGGGACTGA AGAAACACAC 
AAGGCTTTCA ACCATGGCAC CGAGCTCATT CCCTGGTACG TGTTATCGCT GCAGCCGGAT 
GTTCACCAGT TTCTGCTGCA GGGGGCCACG GTCATCCACT ACGACCAGGA CAGCCACCTC 
TCAGCACGCT GTCTGCTGCG GCTGCAGCCC GACAACACAA CGCTCACCTG GGGTAACCCA 
CAGAAAGGTG GGGCCTCCCC CACAGAGCCA CCACTGGGAT TAGGACAGGC CGTCGTCGCA 
GGACTAGCCG AGGGGCTTTT GGACCTTGGA GTGGTGAAGG CAGTGTTCCT GGGTCATCAG 
GGAGTCGATG TTCATGCTGT ATGTCTGCAA AACAAGCTGA GCCACATGAC AGTGGAGGAG 
AACACCCTGA GCCTCCTCTA TGGTGTCAGT ACCACTGACA ACAGACTTCT GCACTTTGTG 
GCTCCAAACC ACACCACGCA AATGCTCCAT AAAGGTTTGT CAGAGTTGGT GACGGCCACA 
CGAAAATTGA AGAAGTTTCC TGATCAGCGG TTGCAGTGGC TGCGGAGGCA GTATGTCAGT 
TTATATCAGG AGGATGGCAG GTATGAAGGG CCCACATTAG CCCAAGCCAT TGAGCTGTTT 
GGAGGCCGGA GATGGAACAT GAGCACAGGT GGAACAGAGA AATCTGCCCA TCAGAAAAAC 
AGTCCTTTGA GCATCAACGA TAAGACCAAG AAGAAGAAGA AGGTCCTCGT CAGGGGAGAC 
AGTGGAGATG CCACTGACGA TGAGATGGTT GCTCGGAAAA CACGGAGCTG TAAGGAAGGA 
ACATATCGAA ACGGACCGGA GTCTGACAGT ATAGATCACG AAGACCCAGA AGATAGCTTC 
CCGGGACCAT TCTCCCTCTC AACCAATAAA AGTCCTGGAT TGCAAGCCTC TTCTTCTTCC 
TCCTCCTCCT CTTCCAGCAT GACTGGCTCC AATCAGTCTC GCCCACAGTC CTCCCCAACC 
CTCTCAGGAA CAGTGAAAGC ACAGCCAGGG GCGTGGAGCA GCAGGTCATG GCATGGTCGT 
GGTAAAGGCT GCTTCAGAGG CTTCCAGAAC CTCATGATCT CTGACAGCAC AATGAGCTTT 
GTTGAGTTTG TGGAGCTTTT CAAGTCATTC AGTATCCGAA GCAGGAAGGA TCTGAAGGAA 
CTGTTTGACA CCTTTGCTGT GCCCTGCATT CGCTCAGATC CAGAGTCTGT TCCTCTTTAC 
ACCAATCTCA GGATAGATGA CAAAGACACA GGTCTACAGC CGGACCTCGA TTTATTAACG 
CGCAATGGTT CGGATCTTGG TCTGTTTATC CGAACGAGGC AGCAGATGTC TGACAACCAG 
AAGCAGATCT CAGACGCCAT TGCAGCAGCC AGCATTGTGA CCAACGGGAC GGGAGTAGAA 
AACGCATCAC TGGGGGTTTT GGGACTGGGC ATCCCTCAAC TTAACGACTT CTTGGTCAAC 
TGTCAGAGAG AACATCTGAG CTATGATGAG ATCCTCAGCA TTATACAGAA GTTTGAACCC 
TCATCAAGCA TGAGACAAAT GGGGTGGATG TCATTCGAAG GCTTTTCAAG GTTCCTGATG 
GATAAGGATA ACTTTGCCTC ACACATTGAG GAATCCCAGA TGAACCCGGA GGAACTGCAG 
CATCCCCTGT CTTATTACTA CATTCAGTCT TCTCACAATA CTTATCTGAC TGGGCACCAG 
CTTAAAGGGG AGTCCTCTGT TGAGCTTTAC AGTCAGGTGC TTTTGCAAGG CTGCAGAAGT 
GTGGAGCTGG ACTGTTGGGA TGGAGATGAT GGCATGCCGG TTATTTATCA CGGACACACT 
CTTACCACCA AGATACCATT TAAGGATGTG GTGGAAGCAA TTAGCAGGTC TGCATTTGTC 
AACTCCAACA TGCCCGTCGT CCTGTCTATA GAGAACCACT GCTCCCTCCC GCAGCAGCGC 
AAGATGGCGG AAATCTTCAA GACGGTGTTT GGGGAGCGTC TGGTGACACG CTTCCTCTTT 
GAAAGCGACT TCAGCGATGA CCCTCACCTG CCATCTCCAC TGCAGCTGCA GGGGAGGATT 
CTCCTCAAGA ATAAGAAGCT GAAAGCACAC CAGGCTCCGG TGGACATACT CAAGCAGAAG 
GCTCACCAGC TTGCCCACAT GCAGGCCCAG GTTAGCAATG GTTCACCAGG GGTTTCCTCT 
CCCGGGACCC AAACCAACGA GGACGAGGAA GAGGAAGAAG ACGAGTACGA CTATGACTAT 
GAGTCCCTCT CAGATGCTGA TGTCCTCACA GCTTCTTCTG CCTCATGTGG CCTGGAAGAC 
AACCTCCTGG ATGACAAACC TGAGGTGAAA AGCTCAGCTG ACAAAGAGGA GCAGCCTGTG 
GATGAAATAC CCAAGAGAAT GAAGAAGCCT GACAACACTA CGCAGAGTAA AGGAAAGGTG 
TTTGACATGG AACTGGGCGA GGAGTTCTAC CTTCCACAGA ACAAAAAGGA AAGTCGACAG 
ATCGCTCAGG AGCTGTCTGA CCTCGTCATC TACTGTCAGG CCGTGAAGTT CCCTGGTCTG 
TCCACGCTGT CTCCGGCTGG CTCCGGCAGA GGGAAAGACC GTGGAAAGAG CAGGAAGTCC 
ATATTTGGCA CGGCTCCAGC TCGCTGCAGC GCAACAGGGG AGGTCACCAC TCCGAGTCGC 
ACCCCTGGGA AAGGTGGCGC CGAGCGCCTG AGCTGGGAAG AGCAGCAAAC GTCTCCTGTC 
CTCAGTCCAC CCACCTCTCT CAGCGCCATT ATCCGTACAC CCAAGTGTTA TCACATCTCC 
TCCGTCAATG AGAACGCCGC CAAGCGACTG TGCCGGCGTT ACTCTCAGAA ACTGATCCAG 
CACACCGTGT GTCAGCTGCT CAGGACTTAC CCAGCAGCCA CCAGGATCGA CTCCACAAAC 
CCAAACCCTC TGCTGTTCTG GCTGCATGGC ATTCAGCTGG TGGCTCTCAA CTATCAGACT 
GACGACCTGC CCATGCAGTT AAACACAGCT CTGTTTGAGG CCAACGGCGG CTGCGGCTAT 
GTCCTGAAAC CAGCGGTGCT GTGGGACCGA AACTGTCCGA TGTATCAGCA GTTCTGTCCG 
ATGGAGAGGG ATGTGGAAAA AATGAGTCCC GCCGTCTACT CCCTTGCTAT CGTTTCGGGG 
CAGAACGTCT GTCCCGGTAA CAGCAGCGGC AGCCCCTGCA TCGAGGTGGA CGTCCTGGGG 
ATGCCGGTCG ACAGCGCCCA CTTCCGTACC AAACCCATCC ACCGCAACAC CCTCAACCCC 
ATGTGGAACG AGCACTTTCA GTTCACCGTG CACTTTGAAG AGATGTGCTT CTTGCGCGTT 
GCTGTTGTGG AGAACAACAG TTCCCAGACT ACTGCTCAGA GAACTCTACC TCTGAAAGCC 
CTCAAGTCAG GTTACAGACA CATCCAGCTG AGGACTCAAC ACAATGAATC TCTGGAAGTG 
TCAAGCTTGT TCATCTATAG CCGAAGGACA GAGGAGTGTC CGACAGGGGG CGACATTCCC 
TCATCCCTGC TGTTCAGCTC TGAGGAGAAG CCTGCATCCC AGCAGCACAG GGTGACGGTG 
TACGGAGCTC CTGGTCCTGA GCCCTTCACT GTGTTCAGTG TGACGGAGCA GATCACTGCC 
AAACAGCTGC TGGACATGGT CGTAGCTTCT GCTGGTGGAA ATCCCTACTT CCTGTGTGAG 
GAGAAGGTTC CTTTGACGAA GGAGCGCAGT GAGACAAAAC GCTGTGCTCA GTATCGCCCC 
CTTGCTCCGG AGGAGGAGGT GGTCCGACTG GTTTCCAGCT GGAGTGCCGA GGAGGGATAT 
GTGGGAAGGA TCTGCTTCAA GCTCAGAGAG GAGAAATTAA ATGAGAAGAA CGCAGCACCA 
GAAGGAGAGG AGGAGTGGAG TGTGGGCGGA GCCAGGGAGG GAGCTGGTGG AGAAGGAGCA 
GCAGCAGCAG AGGATGATGT GTTCCTGGTC CAGATCCATG AAGTTTCTCC AGAGCAGCCT 
CACACTGTGA TCAAGGCCCC GCGCTACAGC ACAGCTCAGG ACATCATCCA GCAGACGTTA 
TTCAAGGCCA AGTATTCCTA CAGCATCCTC TCCAATCCCA ATCCATGCGA CTACGTCCTA 
ATGGAGGAAG TAACCAAAGA CGTGGGCTCA AAGAAGTCCT CCACCACTAA GCCCCTGCAG 
CGGATGCTGC TGGATCACGA GTGCGTCTAT CAGGCTCAGA GCCGCTGGCG GGGCGCGGGG 
AAGTTCATTC TGAAACTGAA AGAGCAGTTG GTGCGGGAAG ACAAAAAGAA AGTCATTTCC 
TTTGCCAGTG AGCTGAAGAA ACTGACGAGC CGCGGCCGCA GCATGACGAC TGGCGGCGGC 
GTGGACGGCC CCGGCCAGAG TAAGGATGAT AGAGCTGCAT GTTGTGTGGC TCTGACAGAG 
CTCCAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plce1 ( XM_029841815.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029697675.1 Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OTo40722
Clone ID Related Accession (Same CDS sequence)	XM_029841815.1
Accession Version	XM_029841815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCATTCACG TTCATTTAAG 
ATGCCCCAAG AAACAAAACG TGCCTTGTTC ATGGTGGGAG ACTTGTCAGA TTCAGACGGA 
GACCTGCCAT CTGAGCCTGC AGTGAAAGAG GGGGAGTTTC AGGGGACTGA AGAAACACAC 
AAGGCTTTCA ACCATGGCAC CGAGCTCATT CCCTGGTACG TGTTATCGCT GCAGCCGGAT 
GTTCACCAGT TTCTGCTGCA GGGGGCCACG GTCATCCACT ACGACCAGGA CAGCCACCTC 
TCAGCACGCT GTCTGCTGCG GCTGCAGCCC GACAACACAA CGCTCACCTG GGGTAACCCA 
CAGAAAGGTG GGGCCTCCCC CACAGAGCCA CCACTGGGAT TAGGACAGGC CGTCGTCGCA 
GGACTAGCCG AGGGGCTTTT GGACCTTGGA GTGGTGAAGG CAGTGTTCCT GGGTCATCAG 
GGAGTCGATG TTCATGCTGT ATGTCTGCAA AACAAGCTGA GCCACATGAC AGTGGAGGAG 
AACACCCTGA GCCTCCTCTA TGGTGTCAGT ACCACTGACA ACAGACTTCT GCACTTTGTG 
GCTCCAAACC ACACCACGCA AATGCTCCAT AAAGGTTTGT CAGAGTTGGT GACGGCCACA 
CGAAAATTGA AGAAGTTTCC TGATCAGCGG TTGCAGTGGC TGCGGAGGCA GTATGTCAGT 
TTATATCAGG AGGATGGCAG GTATGAAGGG CCCACATTAG CCCAAGCCAT TGAGCTGTTT 
GGAGGCCGGA GATGGAACAT GAGCACAGGT GGAACAGAGA AATCTGCCCA TCAGAAAAAC 
AGTCCTTTGA GCATCAACGA TAAGACCAAG AAGAAGAAGA AGGTCCTCGT CAGGGGAGAC 
AGTGGAGATG CCACTGACGA TGAGATGGTT GCTCGGAAAA CACGGAGCTG TAAGGAAGGA 
ACATATCGAA ACGGACCGGA GTCTGACAGT ATAGATCACG AAGACCCAGA AGATAGCTTC 
CCGGGACCAT TCTCCCTCTC AACCAATAAA AGTCCTGGAT TGCAAGCCTC TTCTTCTTCC 
TCCTCCTCCT CTTCCAGCAT GACTGGCTCC AATCAGTCTC GCCCACAGTC CTCCCCAACC 
CTCTCAGGAA CAGTGAAAGC ACAGCCAGGG GCGTGGAGCA GCAGGTCATG GCATGGTCGT 
GGTAAAGGCT GCTTCAGAGG CTTCCAGAAC CTCATGATCT CTGACAGCAC AATGAGCTTT 
GTTGAGTTTG TGGAGCTTTT CAAGTCATTC AGTATCCGAA GCAGGAAGGA TCTGAAGGAA 
CTGTTTGACA CCTTTGCTGT GCCCTGCATT CGCTCAGATC CAGAGTCTGT TCCTCTTTAC 
ACCAATCTCA GGATAGATGA CAAAGACACA GGTCTACAGC CGGACCTCGA TTTATTAACG 
CGCAATGGTT CGGATCTTGG TCTGTTTATC CGAACGAGGC AGCAGATGTC TGACAACCAG 
AAGCAGATCT CAGACGCCAT TGCAGCAGCC AGCATTGTGA CCAACGGGAC GGGAGTAGAA 
AACGCATCAC TGGGGGTTTT GGGACTGGGC ATCCCTCAAC TTAACGACTT CTTGGTCAAC 
TGTCAGAGAG AACATCTGAG CTATGATGAG ATCCTCAGCA TTATACAGAA GTTTGAACCC 
TCATCAAGCA TGAGACAAAT GGGGTGGATG TCATTCGAAG GCTTTTCAAG GTTCCTGATG 
GATAAGGATA ACTTTGCCTC ACACATTGAG GAATCCCAGA TGAACCCGGA GGAACTGCAG 
CATCCCCTGT CTTATTACTA CATTCAGTCT TCTCACAATA CTTATCTGAC TGGGCACCAG 
CTTAAAGGGG AGTCCTCTGT TGAGCTTTAC AGTCAGGTGC TTTTGCAAGG CTGCAGAAGT 
GTGGAGCTGG ACTGTTGGGA TGGAGATGAT GGCATGCCGG TTATTTATCA CGGACACACT 
CTTACCACCA AGATACCATT TAAGGATGTG GTGGAAGCAA TTAGCAGGTC TGCATTTGTC 
AACTCCAACA TGCCCGTCGT CCTGTCTATA GAGAACCACT GCTCCCTCCC GCAGCAGCGC 
AAGATGGCGG AAATCTTCAA GACGGTGTTT GGGGAGCGTC TGGTGACACG CTTCCTCTTT 
GAAAGCGACT TCAGCGATGA CCCTCACCTG CCATCTCCAC TGCAGCTGCA GGGGAGGATT 
CTCCTCAAGA ATAAGAAGCT GAAAGCACAC CAGGCTCCGG TGGACATACT CAAGCAGAAG 
GCTCACCAGC TTGCCCACAT GCAGGCCCAG GTTAGCAATG GTTCACCAGG GGTTTCCTCT 
CCCGGGACCC AAACCAACGA GGACGAGGAA GAGGAAGAAG ACGAGTACGA CTATGACTAT 
GAGTCCCTCT CAGATGCTGA TGTCCTCACA GCTTCTTCTG CCTCATGTGG CCTGGAAGAC 
AACCTCCTGG ATGACAAACC TGAGGTGAAA AGCTCAGCTG ACAAAGAGGA GCAGCCTGTG 
GATGAAATAC CCAAGAGAAT GAAGAAGCCT GACAACACTA CGCAGAGTAA AGGAAAGGTG 
TTTGACATGG AACTGGGCGA GGAGTTCTAC CTTCCACAGA ACAAAAAGGA AAGTCGACAG 
ATCGCTCAGG AGCTGTCTGA CCTCGTCATC TACTGTCAGG CCGTGAAGTT CCCTGGTCTG 
TCCACGCTGT CTCCGGCTGG CTCCGGCAGA GGGAAAGACC GTGGAAAGAG CAGGAAGTCC 
ATATTTGGCA CGGCTCCAGC TCGCTGCAGC GCAACAGGGG AGGTCACCAC TCCGAGTCGC 
ACCCCTGGGA AAGGTGGCGC CGAGCGCCTG AGCTGGGAAG AGCAGCAAAC GTCTCCTGTC 
CTCAGTCCAC CCACCTCTCT CAGCGCCATT ATCCGTACAC CCAAGTGTTA TCACATCTCC 
TCCGTCAATG AGAACGCCGC CAAGCGACTG TGCCGGCGTT ACTCTCAGAA ACTGATCCAG 
CACACCGTGT GTCAGCTGCT CAGGACTTAC CCAGCAGCCA CCAGGATCGA CTCCACAAAC 
CCAAACCCTC TGCTGTTCTG GCTGCATGGC ATTCAGCTGG TGGCTCTCAA CTATCAGACT 
GACGACCTGC CCATGCAGTT AAACACAGCT CTGTTTGAGG CCAACGGCGG CTGCGGCTAT 
GTCCTGAAAC CAGCGGTGCT GTGGGACCGA AACTGTCCGA TGTATCAGCA GTTCTGTCCG 
ATGGAGAGGG ATGTGGAAAA AATGAGTCCC GCCGTCTACT CCCTTGCTAT CGTTTCGGGG 
CAGAACGTCT GTCCCGGTAA CAGCAGCGGC AGCCCCTGCA TCGAGGTGGA CGTCCTGGGG 
ATGCCGGTCG ACAGCGCCCA CTTCCGTACC AAACCCATCC ACCGCAACAC CCTCAACCCC 
ATGTGGAACG AGCACTTTCA GTTCACCGTG CACTTTGAAG AGATGTGCTT CTTGCGCGTT 
GCTGTTGTGG AGAACAACAG TTCCCAGACT ACTGCTCAGA GAACTCTACC TCTGAAAGCC 
CTCAAGTCAG GTTACAGACA CATCCAGCTG AGGACTCAAC ACAATGAATC TCTGGAAGTG 
TCAAGCTTGT TCATCTATAG CCGAAGGACA GAGGAGTGTC CGACAGGGGG CGACATTCCC 
TCATCCCTGC TGTTCAGCTC TGAGGAGAAG CCTGCATCCC AGCAGCACAG GGTGACGGTG 
TACGGAGCTC CTGGTCCTGA GCCCTTCACT GTGTTCAGTG TGACGGAGCA GATCACTGCC 
AAACAGCTGC TGGACATGGT CGTAGCTTCT GCTGGTGGAA ATCCCTACTT CCTGTGTGAG 
GAGAAGGTTC CTTTGACGAA GGAGCGCAGT GAGACAAAAC GCTGTGCTCA GTATCGCCCC 
CTTGCTCCGG AGGAGGAGGT GGTCCGACTG GTTTCCAGCT GGAGTGCCGA GGAGGGATAT 
GTGGGAAGGA TCTGCTTCAA GCTCAGAGAG GAGAAATTAA ATGAGAAGAA CGCAGCACCA 
GAAGGAGAGG AGGAGTGGAG TGTGGGCGGA GCCAGGGAGG GAGCTGGTGG AGAAGGAGCA 
GCAGCAGCAG AGGATGATGT GTTCCTGGTC CAGATCCATG AAGTTTCTCC AGAGCAGCCT 
CACACTGTGA TCAAGGCCCC GCGCTACAGC ACAGCTCAGG ACATCATCCA GCAGCATCCT 
CTCCAATCCC AATCCATGCG ACTACGTCCT AATGGAGGAA GTAACCAAAG ACGTGGGCTC 
AAAGAAGTCC TCCACCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029697675.1
CDS	888..7628
Translation	MSTKASQTPAEAAVVDRCSTAPSSSSKPTREVRNTNANQELQRYYDQLWNSNQRSSPPLIFYRKLPCDGEKGLIPNEALSNACLDRSSGGCEEPYQELSTGTDGIANVVPLISSEGTPCQLDSPGEGGQADRNRTGSSHCSSTMSTPQMIPNIDCCLSHTSSHLSPVHSPCFGSSPTQQKQRRGRRSSLPVSMMAFHKTSCASASEPNSSVSSPCNSPLLNHQQRHREGHFQRYRSKDGYSSLERLNRRPRVNKHSLQKLFPRRASLQTVITTGRSGLSSSPGSRDCSEDSSGSGSDGDGDGDGEELLDHTEFIRNRKERSTVLVRRFYKNNQKMTKSVCTGTRAIVRALPSGHISEEVWKSVGTYRNWRPTKKEIGPILMCGRRKKDEFRVCREMQRLVWPGIMDQMVTYYVQNRKQYPLSHPCQKLLLSCPGCVCLRVSLCPETPITDRADLSPCCRLWRCHPAHPPGVYRACLQRTSLFSLLTGALLEAIAALGARSALPYSFPQGSNSHVVLKERQLANSMTSSNALSPAISGISKELAEMRHLVQFPEEIACILTEQEQQLYQRVFPLDYLCFLTRDLGSPECQSKHHPSLKASLSVPAMSTQSSRHNAVEDLVARFNEVSSWVTWLILTAGSMEEKREVFSYLVNVAKCCWNMGNYNGVMEFLAGLRSRKVLKMWQFMDQTDIETMRSLKDAMAQHESSSEYKKVVTRALNIPGCKVVPFCGVFLKELSDALDGTASIISLKSPLENSEDSIEFVSDYSGQHNFLLRSGPDGLHIPEKEATVSNILQIIRSCNRSLEVEDTDDGSTSPSSSCPLPTSRNNSFKDRCRNHSRSFKMPQETKRALFMVGDLSDSDGDLPSEPAVKEGEFQGTEETHKAFNHGTELIPWYVLSLQPDVHQFLLQGATVIHYDQDSHLSARCLLRLQPDNTTLTWGNPQKGGASPTEPPLGLGQAVVAGLAEGLLDLGVVKAVFLGHQGVDVHAVCLQNKLSHMTVEENTLSLLYGVSTTDNRLLHFVAPNHTTQMLHKGLSELVTATRKLKKFPDQRLQWLRRQYVSLYQEDGRYEGPTLAQAIELFGGRRWNMSTGGTEKSAHQKNSPLSINDKTKKKKKVLVRGDSGDATDDEMVARKTRSCKEGTYRNGPESDSIDHEDPEDSFPGPFSLSTNKSPGLQASSSSSSSSSSMTGSNQSRPQSSPTLSGTVKAQPGAWSSRSWHGRGKGCFRGFQNLMISDSTMSFVEFVELFKSFSIRSRKDLKELFDTFAVPCIRSDPESVPLYTNLRIDDKDTGLQPDLDLLTRNGSDLGLFIRTRQQMSDNQKQISDAIAAASIVTNGTGVENASLGVLGLGIPQLNDFLVNCQREHLSYDEILSIIQKFEPSSSMRQMGWMSFEGFSRFLMDKDNFASHIEESQMNPEELQHPLSYYYIQSSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPVIYHGHTLTTKIPFKDVVEAISRSAFVNSNMPVVLSIENHCSLPQQRKMAEIFKTVFGERLVTRFLFESDFSDDPHLPSPLQLQGRILLKNKKLKAHQAPVDILKQKAHQLAHMQAQVSNGSPGVSSPGTQTNEDEEEEEDEYDYDYESLSDADVLTASSASCGLEDNLLDDKPEVKSSADKEEQPVDEIPKRMKKPDNTTQSKGKVFDMELGEEFYLPQNKKESRQIAQELSDLVIYCQAVKFPGLSTLSPAGSGRGKDRGKSRKSIFGTAPARCSATGEVTTPSRTPGKGGAERLSWEEQQTSPVLSPPTSLSAIIRTPKCYHISSVNENAAKRLCRRYSQKLIQHTVCQLLRTYPAATRIDSTNPNPLLFWLHGIQLVALNYQTDDLPMQLNTALFEANGGCGYVLKPAVLWDRNCPMYQQFCPMERDVEKMSPAVYSLAIVSGQNVCPGNSSGSPCIEVDVLGMPVDSAHFRTKPIHRNTLNPMWNEHFQFTVHFEEMCFLRVAVVENNSSQTTAQRTLPLKALKSGYRHIQLRTQHNESLEVSSLFIYSRRTEECPTGGDIPSSLLFSSEEKPASQQHRVTVYGAPGPEPFTVFSVTEQITAKQLLDMVVASAGGNPYFLCEEKVPLTKERSETKRCAQYRPLAPEEEVVRLVSSWSAEEGYVGRICFKLREEKLNEKNAAPEGEEEWSVGGAREGAGGEGAAAAEDDVFLVQIHEVSPEQPHTVIKAPRYSTAQDIIQQHPLQSQSMRLRPNGGSNQRRGLKEVLHH

Target ORF information:

RefSeq Version	XM_029841815.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029841815.1

ORF Insert Sequence:

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCATTCACG TTCATTTAAG 
ATGCCCCAAG AAACAAAACG TGCCTTGTTC ATGGTGGGAG ACTTGTCAGA TTCAGACGGA 
GACCTGCCAT CTGAGCCTGC AGTGAAAGAG GGGGAGTTTC AGGGGACTGA AGAAACACAC 
AAGGCTTTCA ACCATGGCAC CGAGCTCATT CCCTGGTACG TGTTATCGCT GCAGCCGGAT 
GTTCACCAGT TTCTGCTGCA GGGGGCCACG GTCATCCACT ACGACCAGGA CAGCCACCTC 
TCAGCACGCT GTCTGCTGCG GCTGCAGCCC GACAACACAA CGCTCACCTG GGGTAACCCA 
CAGAAAGGTG GGGCCTCCCC CACAGAGCCA CCACTGGGAT TAGGACAGGC CGTCGTCGCA 
GGACTAGCCG AGGGGCTTTT GGACCTTGGA GTGGTGAAGG CAGTGTTCCT GGGTCATCAG 
GGAGTCGATG TTCATGCTGT ATGTCTGCAA AACAAGCTGA GCCACATGAC AGTGGAGGAG 
AACACCCTGA GCCTCCTCTA TGGTGTCAGT ACCACTGACA ACAGACTTCT GCACTTTGTG 
GCTCCAAACC ACACCACGCA AATGCTCCAT AAAGGTTTGT CAGAGTTGGT GACGGCCACA 
CGAAAATTGA AGAAGTTTCC TGATCAGCGG TTGCAGTGGC TGCGGAGGCA GTATGTCAGT 
TTATATCAGG AGGATGGCAG GTATGAAGGG CCCACATTAG CCCAAGCCAT TGAGCTGTTT 
GGAGGCCGGA GATGGAACAT GAGCACAGGT GGAACAGAGA AATCTGCCCA TCAGAAAAAC 
AGTCCTTTGA GCATCAACGA TAAGACCAAG AAGAAGAAGA AGGTCCTCGT CAGGGGAGAC 
AGTGGAGATG CCACTGACGA TGAGATGGTT GCTCGGAAAA CACGGAGCTG TAAGGAAGGA 
ACATATCGAA ACGGACCGGA GTCTGACAGT ATAGATCACG AAGACCCAGA AGATAGCTTC 
CCGGGACCAT TCTCCCTCTC AACCAATAAA AGTCCTGGAT TGCAAGCCTC TTCTTCTTCC 
TCCTCCTCCT CTTCCAGCAT GACTGGCTCC AATCAGTCTC GCCCACAGTC CTCCCCAACC 
CTCTCAGGAA CAGTGAAAGC ACAGCCAGGG GCGTGGAGCA GCAGGTCATG GCATGGTCGT 
GGTAAAGGCT GCTTCAGAGG CTTCCAGAAC CTCATGATCT CTGACAGCAC AATGAGCTTT 
GTTGAGTTTG TGGAGCTTTT CAAGTCATTC AGTATCCGAA GCAGGAAGGA TCTGAAGGAA 
CTGTTTGACA CCTTTGCTGT GCCCTGCATT CGCTCAGATC CAGAGTCTGT TCCTCTTTAC 
ACCAATCTCA GGATAGATGA CAAAGACACA GGTCTACAGC CGGACCTCGA TTTATTAACG 
CGCAATGGTT CGGATCTTGG TCTGTTTATC CGAACGAGGC AGCAGATGTC TGACAACCAG 
AAGCAGATCT CAGACGCCAT TGCAGCAGCC AGCATTGTGA CCAACGGGAC GGGAGTAGAA 
AACGCATCAC TGGGGGTTTT GGGACTGGGC ATCCCTCAAC TTAACGACTT CTTGGTCAAC 
TGTCAGAGAG AACATCTGAG CTATGATGAG ATCCTCAGCA TTATACAGAA GTTTGAACCC 
TCATCAAGCA TGAGACAAAT GGGGTGGATG TCATTCGAAG GCTTTTCAAG GTTCCTGATG 
GATAAGGATA ACTTTGCCTC ACACATTGAG GAATCCCAGA TGAACCCGGA GGAACTGCAG 
CATCCCCTGT CTTATTACTA CATTCAGTCT TCTCACAATA CTTATCTGAC TGGGCACCAG 
CTTAAAGGGG AGTCCTCTGT TGAGCTTTAC AGTCAGGTGC TTTTGCAAGG CTGCAGAAGT 
GTGGAGCTGG ACTGTTGGGA TGGAGATGAT GGCATGCCGG TTATTTATCA CGGACACACT 
CTTACCACCA AGATACCATT TAAGGATGTG GTGGAAGCAA TTAGCAGGTC TGCATTTGTC 
AACTCCAACA TGCCCGTCGT CCTGTCTATA GAGAACCACT GCTCCCTCCC GCAGCAGCGC 
AAGATGGCGG AAATCTTCAA GACGGTGTTT GGGGAGCGTC TGGTGACACG CTTCCTCTTT 
GAAAGCGACT TCAGCGATGA CCCTCACCTG CCATCTCCAC TGCAGCTGCA GGGGAGGATT 
CTCCTCAAGA ATAAGAAGCT GAAAGCACAC CAGGCTCCGG TGGACATACT CAAGCAGAAG 
GCTCACCAGC TTGCCCACAT GCAGGCCCAG GTTAGCAATG GTTCACCAGG GGTTTCCTCT 
CCCGGGACCC AAACCAACGA GGACGAGGAA GAGGAAGAAG ACGAGTACGA CTATGACTAT 
GAGTCCCTCT CAGATGCTGA TGTCCTCACA GCTTCTTCTG CCTCATGTGG CCTGGAAGAC 
AACCTCCTGG ATGACAAACC TGAGGTGAAA AGCTCAGCTG ACAAAGAGGA GCAGCCTGTG 
GATGAAATAC CCAAGAGAAT GAAGAAGCCT GACAACACTA CGCAGAGTAA AGGAAAGGTG 
TTTGACATGG AACTGGGCGA GGAGTTCTAC CTTCCACAGA ACAAAAAGGA AAGTCGACAG 
ATCGCTCAGG AGCTGTCTGA CCTCGTCATC TACTGTCAGG CCGTGAAGTT CCCTGGTCTG 
TCCACGCTGT CTCCGGCTGG CTCCGGCAGA GGGAAAGACC GTGGAAAGAG CAGGAAGTCC 
ATATTTGGCA CGGCTCCAGC TCGCTGCAGC GCAACAGGGG AGGTCACCAC TCCGAGTCGC 
ACCCCTGGGA AAGGTGGCGC CGAGCGCCTG AGCTGGGAAG AGCAGCAAAC GTCTCCTGTC 
CTCAGTCCAC CCACCTCTCT CAGCGCCATT ATCCGTACAC CCAAGTGTTA TCACATCTCC 
TCCGTCAATG AGAACGCCGC CAAGCGACTG TGCCGGCGTT ACTCTCAGAA ACTGATCCAG 
CACACCGTGT GTCAGCTGCT CAGGACTTAC CCAGCAGCCA CCAGGATCGA CTCCACAAAC 
CCAAACCCTC TGCTGTTCTG GCTGCATGGC ATTCAGCTGG TGGCTCTCAA CTATCAGACT 
GACGACCTGC CCATGCAGTT AAACACAGCT CTGTTTGAGG CCAACGGCGG CTGCGGCTAT 
GTCCTGAAAC CAGCGGTGCT GTGGGACCGA AACTGTCCGA TGTATCAGCA GTTCTGTCCG 
ATGGAGAGGG ATGTGGAAAA AATGAGTCCC GCCGTCTACT CCCTTGCTAT CGTTTCGGGG 
CAGAACGTCT GTCCCGGTAA CAGCAGCGGC AGCCCCTGCA TCGAGGTGGA CGTCCTGGGG 
ATGCCGGTCG ACAGCGCCCA CTTCCGTACC AAACCCATCC ACCGCAACAC CCTCAACCCC 
ATGTGGAACG AGCACTTTCA GTTCACCGTG CACTTTGAAG AGATGTGCTT CTTGCGCGTT 
GCTGTTGTGG AGAACAACAG TTCCCAGACT ACTGCTCAGA GAACTCTACC TCTGAAAGCC 
CTCAAGTCAG GTTACAGACA CATCCAGCTG AGGACTCAAC ACAATGAATC TCTGGAAGTG 
TCAAGCTTGT TCATCTATAG CCGAAGGACA GAGGAGTGTC CGACAGGGGG CGACATTCCC 
TCATCCCTGC TGTTCAGCTC TGAGGAGAAG CCTGCATCCC AGCAGCACAG GGTGACGGTG 
TACGGAGCTC CTGGTCCTGA GCCCTTCACT GTGTTCAGTG TGACGGAGCA GATCACTGCC 
AAACAGCTGC TGGACATGGT CGTAGCTTCT GCTGGTGGAA ATCCCTACTT CCTGTGTGAG 
GAGAAGGTTC CTTTGACGAA GGAGCGCAGT GAGACAAAAC GCTGTGCTCA GTATCGCCCC 
CTTGCTCCGG AGGAGGAGGT GGTCCGACTG GTTTCCAGCT GGAGTGCCGA GGAGGGATAT 
GTGGGAAGGA TCTGCTTCAA GCTCAGAGAG GAGAAATTAA ATGAGAAGAA CGCAGCACCA 
GAAGGAGAGG AGGAGTGGAG TGTGGGCGGA GCCAGGGAGG GAGCTGGTGG AGAAGGAGCA 
GCAGCAGCAG AGGATGATGT GTTCCTGGTC CAGATCCATG AAGTTTCTCC AGAGCAGCCT 
CACACTGTGA TCAAGGCCCC GCGCTACAGC ACAGCTCAGG ACATCATCCA GCAGCATCCT 
CTCCAATCCC AATCCATGCG ACTACGTCCT AATGGAGGAA GTAACCAAAG ACGTGGGCTC 
AAAGAAGTCC TCCACCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plce1 ( XM_011606185.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011604487.2 Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OTo40723
Clone ID Related Accession (Same CDS sequence)	XM_011606185.2
Accession Version	XM_011606185.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011606185.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCAGTTCAT GGTGGGAGAC 
TTGTCAGATT CAGACGGAGA CCTGCCATCT GAGCCTGCAG TGAAAGAGGG GGAGTTTCAG 
GGGACTGAAG AAACACACAA GGCTTTCAAC CATGGCACCG AGCTCATTCC CTGGTACGTG 
TTATCGCTGC AGCCGGATGT TCACCAGTTT CTGCTGCAGG GGGCCACGGT CATCCACTAC 
GACCAGGACA GCCACCTCTC AGCACGCTGT CTGCTGCGGC TGCAGCCCGA CAACACAACG 
CTCACCTGGG GTAACCCACA GAAAGGTGGG GCCTCCCCCA CAGAGCCACC ACTGGGATTA 
GGACAGGCCG TCGTCGCAGG ACTAGCCGAG GGGCTTTTGG ACCTTGGAGT GGTGAAGGCA 
GTGTTCCTGG GTCATCAGGG AGTCGATGTT CATGCTGTAT GTCTGCAAAA CAAGCTGAGC 
CACATGACAG TGGAGGAGAA CACCCTGAGC CTCCTCTATG GTGTCAGTAC CACTGACAAC 
AGACTTCTGC ACTTTGTGGC TCCAAACCAC ACCACGCAAA TGCTCCATAA AGGTTTGTCA 
GAGTTGGTGA CGGCCACACG AAAATTGAAG AAGTTTCCTG ATCAGCGGTT GCAGTGGCTG 
CGGAGGCAGT ATGTCAGTTT ATATCAGGAG GATGGCAGGT ATGAAGGGCC CACATTAGCC 
CAAGCCATTG AGCTGTTTGG AGGCCGGAGA TGGAACATGA GCACAGGTGG AACAGAGAAA 
TCTGCCCATC AGAAAAACAG TCCTTTGAGC ATCAACGATA AGACCAAGAA GAAGAAGAAG 
GTCCTCGTCA GGGGAGACAG TGGAGATGCC ACTGACGATG AGATGGTTGC TCGGAAAACA 
CGGAGCTGTA AGGAAGGAAC ATATCGAAAC GGACCGGAGT CTGACAGTAT AGATCACGAA 
GACCCAGAAG ATAGCTTCCC GGGACCATTC TCCCTCTCAA CCAATAAAAG TCCTGGATTG 
CAAGCCTCTT CTTCTTCCTC CTCCTCCTCT TCCAGCATGA CTGGCTCCAA TCAGTCTCGC 
CCACAGTCCT CCCCAACCCT CTCAGGAACA GTGAAAGCAC AGCCAGGGGC GTGGAGCAGC 
AGGTCATGGC ATGGTCGTGG TAAAGGCTGC TTCAGAGGCT TCCAGAACCT CATGATCTCT 
GACAGCACAA TGAGCTTTGT TGAGTTTGTG GAGCTTTTCA AGTCATTCAG TATCCGAAGC 
AGGAAGGATC TGAAGGAACT GTTTGACACC TTTGCTGTGC CCTGCATTCG CTCAGATCCA 
GAGTCTGTTC CTCTTTACAC CAATCTCAGG ATAGATGACA AAGACACAGG TCTACAGCCG 
GACCTCGATT TATTAACGCG CAATGGTTCG GATCTTGGTC TGTTTATCCG AACGAGGCAG 
CAGATGTCTG ACAACCAGAA GCAGATCTCA GACGCCATTG CAGCAGCCAG CATTGTGACC 
AACGGGACGG GAGTAGAAAA CGCATCACTG GGGGTTTTGG GACTGGGCAT CCCTCAACTT 
AACGACTTCT TGGTCAACTG TCAGAGAGAA CATCTGAGCT ATGATGAGAT CCTCAGCATT 
ATACAGAAGT TTGAACCCTC ATCAAGCATG AGACAAATGG GGTGGATGTC ATTCGAAGGC 
TTTTCAAGGT TCCTGATGGA TAAGGATAAC TTTGCCTCAC ACATTGAGGA ATCCCAGATG 
AACCCGGAGG AACTGCAGCA TCCCCTGTCT TATTACTACA TTCAGTCTTC TCACAATACT 
TATCTGACTG GGCACCAGCT TAAAGGGGAG TCCTCTGTTG AGCTTTACAG TCAGGTGCTT 
TTGCAAGGCT GCAGAAGTGT GGAGCTGGAC TGTTGGGATG GAGATGATGG CATGCCGGTT 
ATTTATCACG GACACACTCT TACCACCAAG ATACCATTTA AGGATGTGGT GGAAGCAATT 
AGCAGGTCTG CATTTGTCAA CTCCAACATG CCCGTCGTCC TGTCTATAGA GAACCACTGC 
TCCCTCCCGC AGCAGCGCAA GATGGCGGAA ATCTTCAAGA CGGTGTTTGG GGAGCGTCTG 
GTGACACGCT TCCTCTTTGA AAGCGACTTC AGCGATGACC CTCACCTGCC ATCTCCACTG 
CAGCTGCAGG GGAGGATTCT CCTCAAGAAT AAGAAGCTGA AAGCACACCA GGCTCCGGTG 
GACATACTCA AGCAGAAGGC TCACCAGCTT GCCCACATGC AGGCCCAGGT TAGCAATGGT 
TCACCAGGGG TTTCCTCTCC CGGGACCCAA ACCAACGAGG ACGAGGAAGA GGAAGAAGAC 
GAGTACGACT ATGACTATGA GTCCCTCTCA GATGACAACC TCCTGGATGA CAAACCTGAG 
GTGAAAAGCT CAGCTGACAA AGAGGAGCAG CCTGTGGATG AAATACCCAA GAGAATGAAG 
AAGCCTGACA ACACTACGCA GAGTAAAGGA AAGGTGTTTG ACATGGAACT GGGCGAGGAG 
TTCTACCTTC CACAGAACAA AAAGGAAAGT CGACAGATCG CTCAGGAGCT GTCTGACCTC 
GTCATCTACT GTCAGGCCGT GAAGTTCCCT GGTCTGTCCA CGCTGTCTCC GGCTGGCTCC 
GGCAGAGGGA AAGACCGTGG AAAGAGCAGG AAGTCCATAT TTGGCACGGC TCCAGCTCGC 
TGCAGCGCAA CAGGGGAGGT CACCACTCCG AGTCGCACCC CTGGGAAAGG TGGCGCCGAG 
CGCCTGAGCT GGGAAGAGCA GCAAACGTCT CCTGTCCTCA GTCCACCCAC CTCTCTCAGC 
GCCATTATCC GTACACCCAA GTGTTATCAC ATCTCCTCCG TCAATGAGAA CGCCGCCAAG 
CGACTGTGCC GGCGTTACTC TCAGAAACTG ATCCAGCACA CCGTGTGTCA GCTGCTCAGG 
ACTTACCCAG CAGCCACCAG GATCGACTCC ACAAACCCAA ACCCTCTGCT GTTCTGGCTG 
CATGGCATTC AGCTGGTGGC TCTCAACTAT CAGACTGACG ACCTGCCCAT GCAGTTAAAC 
ACAGCTCTGT TTGAGGCCAA CGGCGGCTGC GGCTATGTCC TGAAACCAGC GGTGCTGTGG 
GACCGAAACT GTCCGATGTA TCAGCAGTTC TGTCCGATGG AGAGGGATGT GGAAAAAATG 
AGTCCCGCCG TCTACTCCCT TGCTATCGTT TCGGGGCAGA ACGTCTGTCC CGGTAACAGC 
AGCGGCAGCC CCTGCATCGA GGTGGACGTC CTGGGGATGC CGGTCGACAG CGCCCACTTC 
CGTACCAAAC CCATCCACCG CAACACCCTC AACCCCATGT GGAACGAGCA CTTTCAGTTC 
ACCGTGCACT TTGAAGAGAT GTGCTTCTTG CGCGTTGCTG TTGTGGAGAA CAACAGTTCC 
CAGACTACTG CTCAGAGAAC TCTACCTCTG AAAGCCCTCA AGTCAGGTTA CAGACACATC 
CAGCTGAGGA CTCAACACAA TGAATCTCTG GAAGTGTCAA GCTTGTTCAT CTATAGCCGA 
AGGACAGAGG AGTGTCCGAC AGGGGGCGAC ATTCCCTCAT CCCTGCTGTT CAGCTCTGAG 
GAGAAGCCTG CATCCCAGCA GCACAGGGTG ACGGTGTACG GAGCTCCTGG TCCTGAGCCC 
TTCACTGTGT TCAGTGTGAC GGAGCAGATC ACTGCCAAAC AGCTGCTGGA CATGGTCGTA 
GCTTCTGCTG GTGGAAATCC CTACTTCCTG TGTGAGGAGA AGGTTCCTTT GACGAAGGAG 
CGCAGTGAGA CAAAACGCTG TGCTCAGTAT CGCCCCCTTG CTCCGGAGGA GGAGGTGGTC 
CGACTGGTTT CCAGCTGGAG TGCCGAGGAG GGATATGTGG GAAGGATCTG CTTCAAGCTC 
AGAGAGGAGA AATTAAATGA GAAGAACGCA GCACCAGAAG GAGAGGAGGA GTGGAGTGTG 
GGCGGAGCCA GGGAGGGAGC TGGTGGAGAA GGAGCAGCAG CAGCAGAGGA TGATGTGTTC 
CTGGTCCAGA TCCATGAAGT TTCTCCAGAG CAGCCTCACA CTGTGATCAA GGCCCCGCGC 
TACAGCACAG CTCAGGACAT CATCCAGCAG ACGTTATTCA AGGCCAAGTA TTCCTACAGC 
ATCCTCTCCA ATCCCAATCC ATGCGACTAC GTCCTAATGG AGGAAGTAAC CAAAGACGTG 
GGCTCAAAGA AGTCCTCCAC CACTAAGCCC CTGCAGCGGA TGCTGCTGGA TCACGAGTGC 
GTCTATCAGG CTCAGAGCCG CTGGCGGGGC GCGGGGAAGT TCATTCTGAA ACTGAAAGAG 
CAGTTGGTGC GGGAAGACAA AAAGAAAGTC ATTTCCTTTG CCAGTGAGCT GAAGAAACTG 
ACGAGCCGCG GCCGCAGCAT GACGACTGGC GGCGGCGTGG ACGGCCCCGG CCAGAGTAAG 
GATGATAGAG CTGCATGTTG TGTGGCTCTG ACAGAGCTCC AGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604487.2
CDS	605..7552
Translation	MSTKASQTPAEAAVVDRCSTAPSSSSKPTREVRNTNANQELQRYYDQLWNSNQRSSPPLIFYRKLPCDGEKGLIPNEALSNACLDRSSGGCEEPYQELSTGTDGIANVVPLISSEGTPCQLDSPGEGGQADRNRTGSSHCSSTMSTPQMIPNIDCCLSHTSSHLSPVHSPCFGSSPTQQKQRRGRRSSLPVSMMAFHKTSCASASEPNSSVSSPCNSPLLNHQQRHREGHFQRYRSKDGYSSLERLNRRPRVNKHSLQKLFPRRASLQTVITTGRSGLSSSPGSRDCSEDSSGSGSDGDGDGDGEELLDHTEFIRNRKERSTVLVRRFYKNNQKMTKSVCTGTRAIVRALPSGHISEEVWKSVGTYRNWRPTKKEIGPILMCGRRKKDEFRVCREMQRLVWPGIMDQMVTYYVQNRKQYPLSHPCQKLLLSCPGCVCLRVSLCPETPITDRADLSPCCRLWRCHPAHPPGVYRACLQRTSLFSLLTGALLEAIAALGARSALPYSFPQGSNSHVVLKERQLANSMTSSNALSPAISGISKELAEMRHLVQFPEEIACILTEQEQQLYQRVFPLDYLCFLTRDLGSPECQSKHHPSLKASLSVPAMSTQSSRHNAVEDLVARFNEVSSWVTWLILTAGSMEEKREVFSYLVNVAKCCWNMGNYNGVMEFLAGLRSRKVLKMWQFMDQTDIETMRSLKDAMAQHESSSEYKKVVTRALNIPGCKVVPFCGVFLKELSDALDGTASIISLKSPLENSEDSIEFVSDYSGQHNFLLRSGPDGLHIPEKEATVSNILQIIRSCNRSLEVEDTDDGSTSPSSSCPLPTSRNNSFKDRCRNQFMVGDLSDSDGDLPSEPAVKEGEFQGTEETHKAFNHGTELIPWYVLSLQPDVHQFLLQGATVIHYDQDSHLSARCLLRLQPDNTTLTWGNPQKGGASPTEPPLGLGQAVVAGLAEGLLDLGVVKAVFLGHQGVDVHAVCLQNKLSHMTVEENTLSLLYGVSTTDNRLLHFVAPNHTTQMLHKGLSELVTATRKLKKFPDQRLQWLRRQYVSLYQEDGRYEGPTLAQAIELFGGRRWNMSTGGTEKSAHQKNSPLSINDKTKKKKKVLVRGDSGDATDDEMVARKTRSCKEGTYRNGPESDSIDHEDPEDSFPGPFSLSTNKSPGLQASSSSSSSSSSMTGSNQSRPQSSPTLSGTVKAQPGAWSSRSWHGRGKGCFRGFQNLMISDSTMSFVEFVELFKSFSIRSRKDLKELFDTFAVPCIRSDPESVPLYTNLRIDDKDTGLQPDLDLLTRNGSDLGLFIRTRQQMSDNQKQISDAIAAASIVTNGTGVENASLGVLGLGIPQLNDFLVNCQREHLSYDEILSIIQKFEPSSSMRQMGWMSFEGFSRFLMDKDNFASHIEESQMNPEELQHPLSYYYIQSSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPVIYHGHTLTTKIPFKDVVEAISRSAFVNSNMPVVLSIENHCSLPQQRKMAEIFKTVFGERLVTRFLFESDFSDDPHLPSPLQLQGRILLKNKKLKAHQAPVDILKQKAHQLAHMQAQVSNGSPGVSSPGTQTNEDEEEEEDEYDYDYESLSDDNLLDDKPEVKSSADKEEQPVDEIPKRMKKPDNTTQSKGKVFDMELGEEFYLPQNKKESRQIAQELSDLVIYCQAVKFPGLSTLSPAGSGRGKDRGKSRKSIFGTAPARCSATGEVTTPSRTPGKGGAERLSWEEQQTSPVLSPPTSLSAIIRTPKCYHISSVNENAAKRLCRRYSQKLIQHTVCQLLRTYPAATRIDSTNPNPLLFWLHGIQLVALNYQTDDLPMQLNTALFEANGGCGYVLKPAVLWDRNCPMYQQFCPMERDVEKMSPAVYSLAIVSGQNVCPGNSSGSPCIEVDVLGMPVDSAHFRTKPIHRNTLNPMWNEHFQFTVHFEEMCFLRVAVVENNSSQTTAQRTLPLKALKSGYRHIQLRTQHNESLEVSSLFIYSRRTEECPTGGDIPSSLLFSSEEKPASQQHRVTVYGAPGPEPFTVFSVTEQITAKQLLDMVVASAGGNPYFLCEEKVPLTKERSETKRCAQYRPLAPEEEVVRLVSSWSAEEGYVGRICFKLREEKLNEKNAAPEGEEEWSVGGAREGAGGEGAAAAEDDVFLVQIHEVSPEQPHTVIKAPRYSTAQDIIQQTLFKAKYSYSILSNPNPCDYVLMEEVTKDVGSKKSSTTKPLQRMLLDHECVYQAQSRWRGAGKFILKLKEQLVREDKKKVISFASELKKLTSRGRSMTTGGGVDGPGQSKDDRAACCVALTELQE

Target ORF information:

RefSeq Version	XM_011606185.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phospholipase C epsilon 1 (plce1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606185.2

ORF Insert Sequence:

ATGTCAACAA AAGCTAGTCA GACCCCAGCT GAAGCTGCAG TCGTGGATCG GTGCAGCACG 
GCTCCATCGA GTAGCAGCAA ACCCACCAGG GAGGTCAGAA ATACCAACGC CAACCAAGAA 
TTACAGCGAT ACTATGATCA GCTGTGGAAT TCAAATCAGC GGAGCAGCCC ACCGCTGATC 
TTTTACAGGA AGTTGCCCTG TGACGGTGAG AAAGGGCTCA TCCCAAATGA GGCCCTTTCA 
AACGCCTGTT TAGACCGCAG CTCAGGGGGC TGTGAGGAAC CTTATCAAGA GCTCTCAACT 
GGTACTGACG GAATAGCCAA TGTGGTTCCT CTGATAAGTT CAGAGGGAAC ACCGTGCCAG 
CTGGACAGTC CAGGAGAGGG TGGCCAAGCA GACAGAAACA GAACTGGCAG CAGCCACTGC 
TCCTCTACTA TGTCTACCCC TCAAATGATC CCAAATATCG ACTGTTGCCT GAGCCACACA 
TCCAGTCATC TGAGCCCTGT GCACAGCCCT TGCTTCGGCT CCAGCCCCAC GCAGCAAAAA 
CAGCGACGTG GCCGACGCAG CAGCTTGCCC GTGTCCATGA TGGCTTTCCA TAAGACATCC 
TGCGCTTCAG CCAGCGAACC GAACTCTTCA GTGTCATCTC CTTGTAACTC CCCCCTTCTT 
AACCATCAGC AGCGCCATCG AGAAGGCCAC TTCCAACGAT ACCGCAGCAA AGATGGCTAC 
TCCAGTTTGG AGAGGCTCAA CCGAAGGCCC AGGGTTAACA AACACTCCCT CCAGAAATTA 
TTCCCACGGA GGGCATCGCT GCAAACTGTA ATCACCACTG GGAGGAGCGG GCTGTCATCC 
TCACCGGGGA GCAGAGACTG CAGCGAGGAC AGCAGCGGCA GCGGCAGCGA CGGGGACGGG 
GACGGGGACG GGGAGGAACT GTTAGACCAC ACAGAGTTCA TTAGGAACCG TAAAGAACGC 
TCCACTGTCC TGGTGAGGCG CTTCTACAAA AATAATCAAA AGATGACTAA ATCAGTGTGT 
ACTGGGACAA GAGCGATTGT CCGAGCGCTG CCCTCAGGAC ACATATCAGA GGAGGTCTGG 
AAGTCGGTGG GCACTTATAG AAACTGGCGG CCCACTAAGA AGGAGATAGG ACCAATCCTG 
ATGTGTGGCC GAAGAAAGAA AGATGAATTC AGAGTCTGCA GGGAAATGCA GCGTCTGGTC 
TGGCCTGGAA TCATGGACCA AATGGTCACC TACTATGTGC AGAACAGAAA GCAGTATCCT 
CTGAGTCATC CGTGCCAAAA GCTGCTGTTG TCTTGTCCTG GATGTGTGTG TCTCAGGGTG 
TCTCTGTGCC CAGAGACCCC CATCACTGAC AGAGCGGACC TGAGCCCCTG CTGCAGACTG 
TGGCGCTGCC ATCCTGCTCA CCCACCTGGG GTTTATCGTG CTTGCCTCCA GAGGACTTCG 
CTGTTTTCCC TGCTGACTGG AGCCCTGCTG GAGGCTATAG CAGCTCTTGG TGCCCGCTCT 
GCGTTACCCT ACTCTTTCCC TCAGGGCTCG AACAGCCACG TCGTGCTGAA AGAGCGTCAG 
CTGGCCAACA GCATGACCAG CAGCAATGCG CTGTCTCCCG CCATCAGCGG CATCAGTAAG 
GAGCTGGCAG AAATGCGGCA CCTTGTTCAG TTCCCCGAGG AGATCGCCTG CATCCTCACT 
GAGCAGGAGC AGCAGCTCTA CCAGCGGGTT TTCCCCCTGG ACTACCTCTG TTTCCTTACA 
AGAGACCTGG GCAGTCCCGA GTGTCAAAGC AAACATCACC CAAGCCTTAA GGCGTCACTT 
TCTGTGCCAG CCATGTCTAC ACAGAGCTCT CGACACAATG CAGTGGAGGA CCTGGTGGCA 
CGCTTCAATG AGGTGAGCTC ATGGGTAACC TGGCTGATTC TGACAGCAGG GTCCATGGAA 
GAGAAGAGAG AGGTCTTCTC CTATCTGGTC AATGTTGCCA AGTGCTGTTG GAACATGGGC 
AATTACAATG GCGTCATGGA GTTCCTGGCT GGACTCAGGT CCCGTAAGGT GCTGAAAATG 
TGGCAATTTA TGGACCAGAC TGACATCGAG ACCATGCGTA GCCTGAAGGA TGCTATGGCT 
CAACATGAAT CCTCCTCTGA GTACAAGAAG GTTGTGACCA GAGCTCTCAA CATCCCAGGA 
TGTAAGGTGG TGCCTTTCTG TGGGGTCTTT CTAAAGGAGC TGAGTGATGC ACTGGATGGA 
ACAGCAAGCA TAATCAGCCT GAAATCTCCT CTGGAGAATT CAGAAGACTC AATAGAGTTT 
GTTTCAGACT ATAGCGGCCA GCACAACTTC CTGTTGCGGT CTGGTCCTGA TGGTCTACAC 
ATCCCAGAGA AAGAAGCTAC TGTCAGTAAC ATTCTCCAGA TTATCAGATC CTGTAATCGT 
AGTTTGGAGG TGGAAGACAC TGATGATGGC TCAACCAGCC CTTCTTCTAG TTGTCCACTC 
CCCACTTCCA GAAACAACTC CTTCAAGGAC CGCTGCCGTA ATCAGTTCAT GGTGGGAGAC 
TTGTCAGATT CAGACGGAGA CCTGCCATCT GAGCCTGCAG TGAAAGAGGG GGAGTTTCAG 
GGGACTGAAG AAACACACAA GGCTTTCAAC CATGGCACCG AGCTCATTCC CTGGTACGTG 
TTATCGCTGC AGCCGGATGT TCACCAGTTT CTGCTGCAGG GGGCCACGGT CATCCACTAC 
GACCAGGACA GCCACCTCTC AGCACGCTGT CTGCTGCGGC TGCAGCCCGA CAACACAACG 
CTCACCTGGG GTAACCCACA GAAAGGTGGG GCCTCCCCCA CAGAGCCACC ACTGGGATTA 
GGACAGGCCG TCGTCGCAGG ACTAGCCGAG GGGCTTTTGG ACCTTGGAGT GGTGAAGGCA 
GTGTTCCTGG GTCATCAGGG AGTCGATGTT CATGCTGTAT GTCTGCAAAA CAAGCTGAGC 
CACATGACAG TGGAGGAGAA CACCCTGAGC CTCCTCTATG GTGTCAGTAC CACTGACAAC 
AGACTTCTGC ACTTTGTGGC TCCAAACCAC ACCACGCAAA TGCTCCATAA AGGTTTGTCA 
GAGTTGGTGA CGGCCACACG AAAATTGAAG AAGTTTCCTG ATCAGCGGTT GCAGTGGCTG 
CGGAGGCAGT ATGTCAGTTT ATATCAGGAG GATGGCAGGT ATGAAGGGCC CACATTAGCC 
CAAGCCATTG AGCTGTTTGG AGGCCGGAGA TGGAACATGA GCACAGGTGG AACAGAGAAA 
TCTGCCCATC AGAAAAACAG TCCTTTGAGC ATCAACGATA AGACCAAGAA GAAGAAGAAG 
GTCCTCGTCA GGGGAGACAG TGGAGATGCC ACTGACGATG AGATGGTTGC TCGGAAAACA 
CGGAGCTGTA AGGAAGGAAC ATATCGAAAC GGACCGGAGT CTGACAGTAT AGATCACGAA 
GACCCAGAAG ATAGCTTCCC GGGACCATTC TCCCTCTCAA CCAATAAAAG TCCTGGATTG 
CAAGCCTCTT CTTCTTCCTC CTCCTCCTCT TCCAGCATGA CTGGCTCCAA TCAGTCTCGC 
CCACAGTCCT CCCCAACCCT CTCAGGAACA GTGAAAGCAC AGCCAGGGGC GTGGAGCAGC 
AGGTCATGGC ATGGTCGTGG TAAAGGCTGC TTCAGAGGCT TCCAGAACCT CATGATCTCT 
GACAGCACAA TGAGCTTTGT TGAGTTTGTG GAGCTTTTCA AGTCATTCAG TATCCGAAGC 
AGGAAGGATC TGAAGGAACT GTTTGACACC TTTGCTGTGC CCTGCATTCG CTCAGATCCA 
GAGTCTGTTC CTCTTTACAC CAATCTCAGG ATAGATGACA AAGACACAGG TCTACAGCCG 
GACCTCGATT TATTAACGCG CAATGGTTCG GATCTTGGTC TGTTTATCCG AACGAGGCAG 
CAGATGTCTG ACAACCAGAA GCAGATCTCA GACGCCATTG CAGCAGCCAG CATTGTGACC 
AACGGGACGG GAGTAGAAAA CGCATCACTG GGGGTTTTGG GACTGGGCAT CCCTCAACTT 
AACGACTTCT TGGTCAACTG TCAGAGAGAA CATCTGAGCT ATGATGAGAT CCTCAGCATT 
ATACAGAAGT TTGAACCCTC ATCAAGCATG AGACAAATGG GGTGGATGTC ATTCGAAGGC 
TTTTCAAGGT TCCTGATGGA TAAGGATAAC TTTGCCTCAC ACATTGAGGA ATCCCAGATG 
AACCCGGAGG AACTGCAGCA TCCCCTGTCT TATTACTACA TTCAGTCTTC TCACAATACT 
TATCTGACTG GGCACCAGCT TAAAGGGGAG TCCTCTGTTG AGCTTTACAG TCAGGTGCTT 
TTGCAAGGCT GCAGAAGTGT GGAGCTGGAC TGTTGGGATG GAGATGATGG CATGCCGGTT 
ATTTATCACG GACACACTCT TACCACCAAG ATACCATTTA AGGATGTGGT GGAAGCAATT 
AGCAGGTCTG CATTTGTCAA CTCCAACATG CCCGTCGTCC TGTCTATAGA GAACCACTGC 
TCCCTCCCGC AGCAGCGCAA GATGGCGGAA ATCTTCAAGA CGGTGTTTGG GGAGCGTCTG 
GTGACACGCT TCCTCTTTGA AAGCGACTTC AGCGATGACC CTCACCTGCC ATCTCCACTG 
CAGCTGCAGG GGAGGATTCT CCTCAAGAAT AAGAAGCTGA AAGCACACCA GGCTCCGGTG 
GACATACTCA AGCAGAAGGC TCACCAGCTT GCCCACATGC AGGCCCAGGT TAGCAATGGT 
TCACCAGGGG TTTCCTCTCC CGGGACCCAA ACCAACGAGG ACGAGGAAGA GGAAGAAGAC 
GAGTACGACT ATGACTATGA GTCCCTCTCA GATGACAACC TCCTGGATGA CAAACCTGAG 
GTGAAAAGCT CAGCTGACAA AGAGGAGCAG CCTGTGGATG AAATACCCAA GAGAATGAAG 
AAGCCTGACA ACACTACGCA GAGTAAAGGA AAGGTGTTTG ACATGGAACT GGGCGAGGAG 
TTCTACCTTC CACAGAACAA AAAGGAAAGT CGACAGATCG CTCAGGAGCT GTCTGACCTC 
GTCATCTACT GTCAGGCCGT GAAGTTCCCT GGTCTGTCCA CGCTGTCTCC GGCTGGCTCC 
GGCAGAGGGA AAGACCGTGG AAAGAGCAGG AAGTCCATAT TTGGCACGGC TCCAGCTCGC 
TGCAGCGCAA CAGGGGAGGT CACCACTCCG AGTCGCACCC CTGGGAAAGG TGGCGCCGAG 
CGCCTGAGCT GGGAAGAGCA GCAAACGTCT CCTGTCCTCA GTCCACCCAC CTCTCTCAGC 
GCCATTATCC GTACACCCAA GTGTTATCAC ATCTCCTCCG TCAATGAGAA CGCCGCCAAG 
CGACTGTGCC GGCGTTACTC TCAGAAACTG ATCCAGCACA CCGTGTGTCA GCTGCTCAGG 
ACTTACCCAG CAGCCACCAG GATCGACTCC ACAAACCCAA ACCCTCTGCT GTTCTGGCTG 
CATGGCATTC AGCTGGTGGC TCTCAACTAT CAGACTGACG ACCTGCCCAT GCAGTTAAAC 
ACAGCTCTGT TTGAGGCCAA CGGCGGCTGC GGCTATGTCC TGAAACCAGC GGTGCTGTGG 
GACCGAAACT GTCCGATGTA TCAGCAGTTC TGTCCGATGG AGAGGGATGT GGAAAAAATG 
AGTCCCGCCG TCTACTCCCT TGCTATCGTT TCGGGGCAGA ACGTCTGTCC CGGTAACAGC 
AGCGGCAGCC CCTGCATCGA GGTGGACGTC CTGGGGATGC CGGTCGACAG CGCCCACTTC 
CGTACCAAAC CCATCCACCG CAACACCCTC AACCCCATGT GGAACGAGCA CTTTCAGTTC 
ACCGTGCACT TTGAAGAGAT GTGCTTCTTG CGCGTTGCTG TTGTGGAGAA CAACAGTTCC 
CAGACTACTG CTCAGAGAAC TCTACCTCTG AAAGCCCTCA AGTCAGGTTA CAGACACATC 
CAGCTGAGGA CTCAACACAA TGAATCTCTG GAAGTGTCAA GCTTGTTCAT CTATAGCCGA 
AGGACAGAGG AGTGTCCGAC AGGGGGCGAC ATTCCCTCAT CCCTGCTGTT CAGCTCTGAG 
GAGAAGCCTG CATCCCAGCA GCACAGGGTG ACGGTGTACG GAGCTCCTGG TCCTGAGCCC 
TTCACTGTGT TCAGTGTGAC GGAGCAGATC ACTGCCAAAC AGCTGCTGGA CATGGTCGTA 
GCTTCTGCTG GTGGAAATCC CTACTTCCTG TGTGAGGAGA AGGTTCCTTT GACGAAGGAG 
CGCAGTGAGA CAAAACGCTG TGCTCAGTAT CGCCCCCTTG CTCCGGAGGA GGAGGTGGTC 
CGACTGGTTT CCAGCTGGAG TGCCGAGGAG GGATATGTGG GAAGGATCTG CTTCAAGCTC 
AGAGAGGAGA AATTAAATGA GAAGAACGCA GCACCAGAAG GAGAGGAGGA GTGGAGTGTG 
GGCGGAGCCA GGGAGGGAGC TGGTGGAGAA GGAGCAGCAG CAGCAGAGGA TGATGTGTTC 
CTGGTCCAGA TCCATGAAGT TTCTCCAGAG CAGCCTCACA CTGTGATCAA GGCCCCGCGC 
TACAGCACAG CTCAGGACAT CATCCAGCAG ACGTTATTCA AGGCCAAGTA TTCCTACAGC 
ATCCTCTCCA ATCCCAATCC ATGCGACTAC GTCCTAATGG AGGAAGTAAC CAAAGACGTG 
GGCTCAAAGA AGTCCTCCAC CACTAAGCCC CTGCAGCGGA TGCTGCTGGA TCACGAGTGC 
GTCTATCAGG CTCAGAGCCG CTGGCGGGGC GCGGGGAAGT TCATTCTGAA ACTGAAAGAG 
CAGTTGGTGC GGGAAGACAA AAAGAAAGTC ATTTCCTTTG CCAGTGAGCT GAAGAAACTG 
ACGAGCCGCG GCCGCAGCAT GACGACTGGC GGCGGCGTGG ACGGCCCCGG CCAGAGTAAG 
GATGATAGAG CTGCATGTTG TGTGGCTCTG ACAGAGCTCC AGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plce1 ( XM_011606166.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011604468.1 Takifugu rubripes phospholipase C, epsilon 1 (plce1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo22205
Clone ID Related Accession (Same CDS sequence)	XM_011606166.1
Accession Version	XM_011606166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070902.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAAAT CAGTGTGTAC TGGGACAAGA GCGATTGTCC GAGCGCTGCC CTCAGGACAC 
ATATCAGAGG AGGTCTGGAA GTCGGTGGGC ACTTATAGAA ACTGGCGTCC TACTAAGAAG 
GAGATAGGAC CAATCCTGAT GTGTGGCCGA AGAAAGAAAG ATGAATTCAG AGTGTGCAGG 
GAAATGCAGC GTCTGGTCTG GCCTGGAATC ATGGACCAAA TGGTCACCTA CTATGTGCAG 
AACAGAAAGC AGTATCCTCT GAGTCATCCA TGCCAAAAGC TGCTGTTGTC TTGTCCTGGA 
TGTGTGTGTC TCAGGGTGTC TCTGTGCCCA GAGACCCCCA TCACTGACAG AGCGGACCTG 
AGCCCCTGCT GCAGACTGTG GCGCTGCCAT CCTGCTCACC CACCTGGGGT TTATCGTGCT 
TGCCTCCAGA GGACTTCGCT GTTTTCCCTG CTGACTGGAG CCCTGCTGGA GGCTATAGCA 
GCTCTTGGTG CCCGCTCTGC GTTACCCTAC TCTTTCCCTC AGGGCTCGAA CAGCCACGTC 
GTGCTGAAAG AGCGTCAGCT GGCCAACAGC ATGACCAGCA GCAGTGCGCT GTCTCCCGCC 
GTCAGCGGCA TCAGTAAGGA GCTGGCAGAA ATGCGGCACC TTGTTCAGTT CCCCGAGGAG 
ATCGCCTGCA TCCTCACTGA GCAGGAGCAG CAGCTCTACC AGCGGGTTTT CCCCCTGGAC 
TACCTCTGTT TCCTTACAAG AGACCTGGGC AGTCCCGAGT GTCAAAGCAA ACATCACCCA 
AGCCTTAAGG CGTCACTTTC TGTGCCAGCC ATGTCTACAC AGAGCTCTCG ACACAATGCA 
GTGGAGGACC TGGTGGCACG CTTCAATGAG GTGAGCTCAT GGGTAACCTG GCTGATTCTG 
ACAGCAGGGT CCATGGAAGA GAAGAGAGAG GTCTTCTCCT ATCTGGTCAA TGTTGCCAAG 
TGCTGTTGGA ACATGGGCAA TTACAATGGC GTCATGGAGT TCCTGGCTGG ACTCAGGTCC 
CGTAAGGTGC TGAAAATGTG GCAATTTATG GACCAGACTG ACATCGAGAC CATGCGTAGC 
CTGAAGGATG CTATGGCTCA ACATGAATCC TCCTCTGAGT ACAAGAAGGT TGTGACCAGA 
GCTCTCAACA TCCCAGGATG TAAGGTGGTG CCTTTCTGTG GGGTCTTTCT AAAGGAGCTG 
AGTGATGCAC TGGATGGAAC AGCAAGCATA ATCAGCCTGA AATCTCCTCT GGAGAATTCA 
GAAGACTCAA TAGAGTTTGT TTCAGACTAT AGCGGCCAGC ACAACTTCCT GTTGCGGTCT 
GGTCCTGATG GTCTACACAT CCCAGAGAAA GAAGCTACTG TCAGTAACAT TCTCCAGATT 
ATCAGATCCT GTAATCGTAG TTTGGAGGTG GAAGACACTG ATGATGGCTC AACCAGCCCT 
TCTTCTAGTT GTCCACTCCC CACTTCCAGA AACAACTCCT TCAAGGACCG CTGCCGTAAT 
CAGTTCATGG TGGGAGACTT GTCAGATTCA GACGGAGACC TGCCATCTGA GCCTGCAGTG 
AAAGAGGGGG AGTTTCAGGG GACTGAAGAA ACACACAAGG CTTTCAACCA TGGCACCGAG 
CTCATTCCCT GGTACGTGTT ATCGCTGCAG CCGGATGTTC ACCAGTTTCT GCTGCAGGGG 
GCCACGGTCA TCCACTACGA CCAGGACAGC CACCTCTCAG CACGCTGTCT GCTGCGGCTG 
CAGCCCGACA ACACAACGCT CACCTGGGGT AACCCACAGA AAGGTGGGGC CTCCCCCACA 
GAGCCACCAC TGGGATTAGG ACAGGCCGTC GTCGCAGGAC TAGCCGAGGG GCTTTTGGAC 
CTTGGAGTGG TGAAGGCAGT GTTCCTAGGT CATCAGGGAG TCGATGTTCA TGCTGTATGT 
CTGCAAAACA AGCTGAGCCA CATGACAGTG GAGGAGAACA CCCTGAGCCT CCTCTATGGT 
GTCAGTACCA CTGACAACAG ACTTCTGCAC TTTGTGGCTC CAAACCACAC CACGCAAATG 
CTCCATAAAG GTTTGTCAGA GTTGGTGACG GCCACACGGA AATTGAAGAA GTTTCCTGAT 
CAGCGGTTGC AGTGGCTGCG GAGGCAGTAT GTCAGTTTAT ATCAGGAGGA TGGCAGGTAT 
GAAGGGCCCA CATTAGCCCA AGCCATTGAG CTGTTTGGAG GCCGGAGATG GAACATGAGC 
ACAGGTGGAA CAGAGAAATC TGCCCATCAG AAAAACAGTC CTTTGAGCAT CAACGATAAG 
ACCAAGAAGA AGAAGAAGGT CCTCGTCAGG GGAGACAGTG GAGATGCCAC TGACGATGAG 
ATGGTTGCTC GGAAAACACG GAGCTGTAAG GAAGGAACAT ATCGAAACGG ACCGGAGTCT 
GACAGTATAG ATCACGAAGA CCCAGAAGAT AGCTTCCCGG GACCATTCTC CCTCTCAACC 
AATAAAAGTC CTGGATTGCA AGCCTCTTCT TCTTCCTCCT CCTCCTCTTC CAGCATGACT 
GGCTCCAATC AGTCTCGCCC ACAGTCCTCC CCAACCCTCT CAGGAACAGT GAAAGCACAG 
CCAGGGGCGT GGAGCAGCAG GTCATGGCAT GGTCGTGGTA AAGGCTGCTT CAGAGGCTTC 
CAGAACCTCA TGATCTCTGA CAGCACAATG AGCTTTGTTG AGTTTGTGGA GCTTTTCAAG 
TCATTCAGTA TCCGAAGCAG GAAGGATCTG AAGGAACTGT TTGACACCTT TGCTGTGCCC 
TGCATTCGCT CAGATCCAGA GTCTGTTCCT CTTTACACCA ATCTCAGGAT AGATGACAAA 
GACACAGGTC TACAGCCGGA CCTCGATTTA TTAACGCGCA ATGGTTCGGA TCTTGGTCTG 
TTTATCCGAA CGAGGCAGCA GATGTCTGAC AACCAGAAGC AGATCTCAGA CGCCATTGCA 
GCAGCCAGCA TTGTGACCAA CGGGACGGGA GTAGAAAACG CATCACTGGG GGTTTTGGGA 
CTGGGCATCC CTCAACTTAA CGACTTCTTG GTCAACTGTC AGAGAGAACA TCTGAGCTAT 
GATGAGATCC TCAGCATTAT ACAGAAGTTT GAACCCTCAT CAAGCATGAG ACAAATGGGG 
TGGATGTCAT TCGAAGGCTT TTCAAGGTTC CTGATGGATA AGGATAACTT TGCCTCACAC 
ATTGAGGAAT CCCAGATGAA CCCGGAGGAA CTGCAGCATC CCCTGTCTTA TTACTACATT 
CAGTCTTCTC ACAATACTTA TCTGACTGGG CACCAGCTTA AAGGGGAGTC CTCTGTTGAG 
CTTTACAGTC AGGTGCTTTT GCAAGGCTGC AGAAGTGTGG AGCTGGACTG TTGGGATGGA 
GATGATGGCA TGCCGGTTAT TTATCACGGA CACACTCTTA CCACCAAGAT ACCATTTAAG 
GATGTGGTGG AAGCAATTAG CAGGTCTGCA TTTGTCAACT CCAACATGCC CGTCGTCCTG 
TCTATAGAGA ACCACTGCTC CCTCCCGCAG CAGCGCAAGA TGGCGGAAAT CTTCAAGACG 
GTGTTTGGGG AGCGTCTGGT GACACGCTTC CTCTTTGAAA GCGACTTCAG CGATGACCCT 
CACCTGCCAT CTCCACTGCA GCTGCAGGGG AGGATTCTCC TCAAGAATAA GAAGCTGAAA 
GCACACCAGG CTCCGGTGGA CATACTCAAG CAGAAGGCTC ACCAGCTTGC CCACATGCAG 
GCCCAGGTTA GCAATGGTTC ACCAGGGGTT TCCTCTCCCG GGACCCAAAC CAACGAGGAC 
GAGGAAGAGG AAGAAGACGA GTACGACTAT GACTATGAGT CTCTCTCAGA TGACAACCTC 
CTGGATGACA AACCTGAGGT GAAAAGCTCA GCTGACAAAG AGGAGCAGCC TGTGGATGAA 
ATACCCAAGA GAATGAAGAA GCCTGACAAC ACTACGCAGA GTAAAGGAAA GGTGTTTGAC 
ATGGAACTGG GCGAGGAGTT CTACCTTCCA CAGAACAAAA AGGAAAGTCG ACAGATCGCT 
CAGGAGCTGT CTGACCTCGT CATCTACTGT CAGGCCGTGA AGTTCCCTGG TCTGTCCACG 
CTGTCTCCGG CTGGCTCCGG CAGAGGGAAA GACCGTGGAA AGAGCAGGAA GTCCATATTT 
GGCACGGCTC CAGCTCGCTG CAGCGCAACA GGGGAGGTCA CCACTCCGAG TCGCACCCCT 
GGGAAAGGTG GCGCCGAGCG CCTGAGCTGG GAAGAGCAGC AAACGTCTCC TGTCCTCAGT 
CCACCCACCT CTCTCAGCGC CATTATCCGT ACACCCAAGT GTTATCACAT CTCCTCCGTC 
AATGAGAACG CCGCCAAGCG ACTGTGCCGG CGTTACTCTC AGAAACTGAT CCAGCACACC 
GTGTGTCAGC TGCTCAGGAC TTACCCAGCA GCCACCAGGA TCGACTCCAC AAACCCAAAC 
CCTCTGCTGT TCTGGCTGCA TGGCATTCAG CTGGTGGCTC TCAACTATCA GACTGACGAC 
CTGCCCATGC AGTTAAACAC AGCTCTGTTT GAGGCCAACG GCGGCTGCGG CTATGTCCTG 
AAACCAGCGG TGCTGTGGGA CCGAAACTGT CCGATGTATC AGCAGTTCTG TCCGATGGAG 
AGGGATGTGG AAAAAATGAG TCCTGCCGTC TACTCCCTTG CTATCGTTTC GGGGCAGAAC 
GTCTGTCCCG GTAACAGCAG CGGCAGCCCC TGCATCGAGG TGGACGTCCT GGGGATGCCG 
GTCGACAGCG CCCACTTCCG TACCAAACCC ATCCACCGCA ACACCCTCAA CCCCATGTGG 
AACGAGCACT TTCAGTTCAC CGTGCACTTT GAAGAGATGT GCTTCTTGCG CGTTGCTGTT 
GTGGAGAACA ACAGTTCCCA GACTACTGCT CAGAGAACTC TACCTCTGAA AGCCCTCAAG 
TCAGGTTACA GACACATCCA GCTGAGGACT CAACACAATG AATCTCTGGA AGTGTCAAGC 
TTGTTCATCT ATAGCCGAAG GACAGAGGAG TGTCCGACAG GGGGCGACAT TCCCTCATCC 
CTGCTGTTCA GCTCTGAGGA GAAGCCTGCA TCCCAGCAGC ACAGGGTGAC GGTGTACGGA 
GCTCCTGGTC CTGAGCCCTT CACTGTGTTC AGTGTGACGG AGCAGATCAC TGCCAAACAG 
CTGCTGGACA TGGTCGTAGC TTCTGCTGGT GGAAATCCCT ACTTCCTGTG TGAGGAGAAG 
GTTCCTTTGA CGAAGGAGCG CAGTGAGACA AAACGCTGTG CTCAGTATCG CCCCCTTGCT 
CCGGAGGAGG AGGTGGTCCG ACTGGTTTCC AGCTGGAGTG CCGAGGAGGG ATATGTGGGA 
AGGATCTGCT TCAAGCTCAG AGAGGAGAAA TTAAATGAGA AGAACGCAGC ACCAGAAGGA 
GAGGAGGAGT GGAGTGTGGG CGGAGCCAGG GAGGGAGCTG GTGGAGAAGG AGCAGCAGCA 
GCAGAGGATG ATGTGTTCCT GGTCCAGATC CATGAAGTTT CTCCAGAGCA GCCTCACACT 
GTGATCAAGG CCCCGCGCTA CAGCACAGCT CAGGACATCA TCCAGCAGAC GTTATTCAAG 
GCCAAGTATT CCTACAGCAT CCTCTCCAAT CCCAATCCAT GCGACTACGT CCTAATGGAG 
GAAGTAACCA AAGACGTGGG CTCAAAGAAG TCCTCCACCA CTAAGCCCCT GCAGCGGATG 
CTGCTGGATC ACGAGTGCGT CTATCAGGCT CAGAGCCGCT GGCGGGGCGC GGGGAAGTTC 
ATTCTGAAAC TGAAAGAGCA GTTGGTGCGG GAAGACAAAA AGAAAGTCAT TTCCTTTGCC 
AGTGAGCTGA AGAAACTGAC GAGCCGCGGC CGCAGCATGA CGACTGGCGG CGGCGTGGAC 
GGCCCCGGCC AGAGGTGTTG GTCTGCAGCA GTCGGTCTGA AGGTGTGGGT GGCAGATGGA 
AGGGTGGGGG CAGGTAGATT CCTTCCATCT TCCTCCTCTG GGAGACGTCT TCATCCAATC 
CACCTGCCCA CCTCCTCTCT GACTGCACCA GTGTTGAAGC TGCACCTCCT CAGGAACTGG 
AAGATCCACA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604468.1
CDS	89..6163
Translation	MTKSVCTGTRAIVRALPSGHISEEVWKSVGTYRNWRPTKKEIGPILMCGRRKKDEFRVCREMQRLVWPGIMDQMVTYYVQNRKQYPLSHPCQKLLLSCPGCVCLRVSLCPETPITDRADLSPCCRLWRCHPAHPPGVYRACLQRTSLFSLLTGALLEAIAALGARSALPYSFPQGSNSHVVLKERQLANSMTSSSALSPAVSGISKELAEMRHLVQFPEEIACILTEQEQQLYQRVFPLDYLCFLTRDLGSPECQSKHHPSLKASLSVPAMSTQSSRHNAVEDLVARFNEVSSWVTWLILTAGSMEEKREVFSYLVNVAKCCWNMGNYNGVMEFLAGLRSRKVLKMWQFMDQTDIETMRSLKDAMAQHESSSEYKKVVTRALNIPGCKVVPFCGVFLKELSDALDGTASIISLKSPLENSEDSIEFVSDYSGQHNFLLRSGPDGLHIPEKEATVSNILQIIRSCNRSLEVEDTDDGSTSPSSSCPLPTSRNNSFKDRCRNQFMVGDLSDSDGDLPSEPAVKEGEFQGTEETHKAFNHGTELIPWYVLSLQPDVHQFLLQGATVIHYDQDSHLSARCLLRLQPDNTTLTWGNPQKGGASPTEPPLGLGQAVVAGLAEGLLDLGVVKAVFLGHQGVDVHAVCLQNKLSHMTVEENTLSLLYGVSTTDNRLLHFVAPNHTTQMLHKGLSELVTATRKLKKFPDQRLQWLRRQYVSLYQEDGRYEGPTLAQAIELFGGRRWNMSTGGTEKSAHQKNSPLSINDKTKKKKKVLVRGDSGDATDDEMVARKTRSCKEGTYRNGPESDSIDHEDPEDSFPGPFSLSTNKSPGLQASSSSSSSSSSMTGSNQSRPQSSPTLSGTVKAQPGAWSSRSWHGRGKGCFRGFQNLMISDSTMSFVEFVELFKSFSIRSRKDLKELFDTFAVPCIRSDPESVPLYTNLRIDDKDTGLQPDLDLLTRNGSDLGLFIRTRQQMSDNQKQISDAIAAASIVTNGTGVENASLGVLGLGIPQLNDFLVNCQREHLSYDEILSIIQKFEPSSSMRQMGWMSFEGFSRFLMDKDNFASHIEESQMNPEELQHPLSYYYIQSSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPVIYHGHTLTTKIPFKDVVEAISRSAFVNSNMPVVLSIENHCSLPQQRKMAEIFKTVFGERLVTRFLFESDFSDDPHLPSPLQLQGRILLKNKKLKAHQAPVDILKQKAHQLAHMQAQVSNGSPGVSSPGTQTNEDEEEEEDEYDYDYESLSDDNLLDDKPEVKSSADKEEQPVDEIPKRMKKPDNTTQSKGKVFDMELGEEFYLPQNKKESRQIAQELSDLVIYCQAVKFPGLSTLSPAGSGRGKDRGKSRKSIFGTAPARCSATGEVTTPSRTPGKGGAERLSWEEQQTSPVLSPPTSLSAIIRTPKCYHISSVNENAAKRLCRRYSQKLIQHTVCQLLRTYPAATRIDSTNPNPLLFWLHGIQLVALNYQTDDLPMQLNTALFEANGGCGYVLKPAVLWDRNCPMYQQFCPMERDVEKMSPAVYSLAIVSGQNVCPGNSSGSPCIEVDVLGMPVDSAHFRTKPIHRNTLNPMWNEHFQFTVHFEEMCFLRVAVVENNSSQTTAQRTLPLKALKSGYRHIQLRTQHNESLEVSSLFIYSRRTEECPTGGDIPSSLLFSSEEKPASQQHRVTVYGAPGPEPFTVFSVTEQITAKQLLDMVVASAGGNPYFLCEEKVPLTKERSETKRCAQYRPLAPEEEVVRLVSSWSAEEGYVGRICFKLREEKLNEKNAAPEGEEEWSVGGAREGAGGEGAAAAEDDVFLVQIHEVSPEQPHTVIKAPRYSTAQDIIQQTLFKAKYSYSILSNPNPCDYVLMEEVTKDVGSKKSSTTKPLQRMLLDHECVYQAQSRWRGAGKFILKLKEQLVREDKKKVISFASELKKLTSRGRSMTTGGGVDGPGQRCWSAAVGLKVWVADGRVGAGRFLPSSSSGRRLHPIHLPTSSLTAPVLKLHLLRNWKIHK

Target ORF information:

RefSeq Version	XM_011606166.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phospholipase C, epsilon 1 (plce1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606166.1

ORF Insert Sequence:

ATGACTAAAT CAGTGTGTAC TGGGACAAGA GCGATTGTCC GAGCGCTGCC CTCAGGACAC 
ATATCAGAGG AGGTCTGGAA GTCGGTGGGC ACTTATAGAA ACTGGCGTCC TACTAAGAAG 
GAGATAGGAC CAATCCTGAT GTGTGGCCGA AGAAAGAAAG ATGAATTCAG AGTGTGCAGG 
GAAATGCAGC GTCTGGTCTG GCCTGGAATC ATGGACCAAA TGGTCACCTA CTATGTGCAG 
AACAGAAAGC AGTATCCTCT GAGTCATCCA TGCCAAAAGC TGCTGTTGTC TTGTCCTGGA 
TGTGTGTGTC TCAGGGTGTC TCTGTGCCCA GAGACCCCCA TCACTGACAG AGCGGACCTG 
AGCCCCTGCT GCAGACTGTG GCGCTGCCAT CCTGCTCACC CACCTGGGGT TTATCGTGCT 
TGCCTCCAGA GGACTTCGCT GTTTTCCCTG CTGACTGGAG CCCTGCTGGA GGCTATAGCA 
GCTCTTGGTG CCCGCTCTGC GTTACCCTAC TCTTTCCCTC AGGGCTCGAA CAGCCACGTC 
GTGCTGAAAG AGCGTCAGCT GGCCAACAGC ATGACCAGCA GCAGTGCGCT GTCTCCCGCC 
GTCAGCGGCA TCAGTAAGGA GCTGGCAGAA ATGCGGCACC TTGTTCAGTT CCCCGAGGAG 
ATCGCCTGCA TCCTCACTGA GCAGGAGCAG CAGCTCTACC AGCGGGTTTT CCCCCTGGAC 
TACCTCTGTT TCCTTACAAG AGACCTGGGC AGTCCCGAGT GTCAAAGCAA ACATCACCCA 
AGCCTTAAGG CGTCACTTTC TGTGCCAGCC ATGTCTACAC AGAGCTCTCG ACACAATGCA 
GTGGAGGACC TGGTGGCACG CTTCAATGAG GTGAGCTCAT GGGTAACCTG GCTGATTCTG 
ACAGCAGGGT CCATGGAAGA GAAGAGAGAG GTCTTCTCCT ATCTGGTCAA TGTTGCCAAG 
TGCTGTTGGA ACATGGGCAA TTACAATGGC GTCATGGAGT TCCTGGCTGG ACTCAGGTCC 
CGTAAGGTGC TGAAAATGTG GCAATTTATG GACCAGACTG ACATCGAGAC CATGCGTAGC 
CTGAAGGATG CTATGGCTCA ACATGAATCC TCCTCTGAGT ACAAGAAGGT TGTGACCAGA 
GCTCTCAACA TCCCAGGATG TAAGGTGGTG CCTTTCTGTG GGGTCTTTCT AAAGGAGCTG 
AGTGATGCAC TGGATGGAAC AGCAAGCATA ATCAGCCTGA AATCTCCTCT GGAGAATTCA 
GAAGACTCAA TAGAGTTTGT TTCAGACTAT AGCGGCCAGC ACAACTTCCT GTTGCGGTCT 
GGTCCTGATG GTCTACACAT CCCAGAGAAA GAAGCTACTG TCAGTAACAT TCTCCAGATT 
ATCAGATCCT GTAATCGTAG TTTGGAGGTG GAAGACACTG ATGATGGCTC AACCAGCCCT 
TCTTCTAGTT GTCCACTCCC CACTTCCAGA AACAACTCCT TCAAGGACCG CTGCCGTAAT 
CAGTTCATGG TGGGAGACTT GTCAGATTCA GACGGAGACC TGCCATCTGA GCCTGCAGTG 
AAAGAGGGGG AGTTTCAGGG GACTGAAGAA ACACACAAGG CTTTCAACCA TGGCACCGAG 
CTCATTCCCT GGTACGTGTT ATCGCTGCAG CCGGATGTTC ACCAGTTTCT GCTGCAGGGG 
GCCACGGTCA TCCACTACGA CCAGGACAGC CACCTCTCAG CACGCTGTCT GCTGCGGCTG 
CAGCCCGACA ACACAACGCT CACCTGGGGT AACCCACAGA AAGGTGGGGC CTCCCCCACA 
GAGCCACCAC TGGGATTAGG ACAGGCCGTC GTCGCAGGAC TAGCCGAGGG GCTTTTGGAC 
CTTGGAGTGG TGAAGGCAGT GTTCCTAGGT CATCAGGGAG TCGATGTTCA TGCTGTATGT 
CTGCAAAACA AGCTGAGCCA CATGACAGTG GAGGAGAACA CCCTGAGCCT CCTCTATGGT 
GTCAGTACCA CTGACAACAG ACTTCTGCAC TTTGTGGCTC CAAACCACAC CACGCAAATG 
CTCCATAAAG GTTTGTCAGA GTTGGTGACG GCCACACGGA AATTGAAGAA GTTTCCTGAT 
CAGCGGTTGC AGTGGCTGCG GAGGCAGTAT GTCAGTTTAT ATCAGGAGGA TGGCAGGTAT 
GAAGGGCCCA CATTAGCCCA AGCCATTGAG CTGTTTGGAG GCCGGAGATG GAACATGAGC 
ACAGGTGGAA CAGAGAAATC TGCCCATCAG AAAAACAGTC CTTTGAGCAT CAACGATAAG 
ACCAAGAAGA AGAAGAAGGT CCTCGTCAGG GGAGACAGTG GAGATGCCAC TGACGATGAG 
ATGGTTGCTC GGAAAACACG GAGCTGTAAG GAAGGAACAT ATCGAAACGG ACCGGAGTCT 
GACAGTATAG ATCACGAAGA CCCAGAAGAT AGCTTCCCGG GACCATTCTC CCTCTCAACC 
AATAAAAGTC CTGGATTGCA AGCCTCTTCT TCTTCCTCCT CCTCCTCTTC CAGCATGACT 
GGCTCCAATC AGTCTCGCCC ACAGTCCTCC CCAACCCTCT CAGGAACAGT GAAAGCACAG 
CCAGGGGCGT GGAGCAGCAG GTCATGGCAT GGTCGTGGTA AAGGCTGCTT CAGAGGCTTC 
CAGAACCTCA TGATCTCTGA CAGCACAATG AGCTTTGTTG AGTTTGTGGA GCTTTTCAAG 
TCATTCAGTA TCCGAAGCAG GAAGGATCTG AAGGAACTGT TTGACACCTT TGCTGTGCCC 
TGCATTCGCT CAGATCCAGA GTCTGTTCCT CTTTACACCA ATCTCAGGAT AGATGACAAA 
GACACAGGTC TACAGCCGGA CCTCGATTTA TTAACGCGCA ATGGTTCGGA TCTTGGTCTG 
TTTATCCGAA CGAGGCAGCA GATGTCTGAC AACCAGAAGC AGATCTCAGA CGCCATTGCA 
GCAGCCAGCA TTGTGACCAA CGGGACGGGA GTAGAAAACG CATCACTGGG GGTTTTGGGA 
CTGGGCATCC CTCAACTTAA CGACTTCTTG GTCAACTGTC AGAGAGAACA TCTGAGCTAT 
GATGAGATCC TCAGCATTAT ACAGAAGTTT GAACCCTCAT CAAGCATGAG ACAAATGGGG 
TGGATGTCAT TCGAAGGCTT TTCAAGGTTC CTGATGGATA AGGATAACTT TGCCTCACAC 
ATTGAGGAAT CCCAGATGAA CCCGGAGGAA CTGCAGCATC CCCTGTCTTA TTACTACATT 
CAGTCTTCTC ACAATACTTA TCTGACTGGG CACCAGCTTA AAGGGGAGTC CTCTGTTGAG 
CTTTACAGTC AGGTGCTTTT GCAAGGCTGC AGAAGTGTGG AGCTGGACTG TTGGGATGGA 
GATGATGGCA TGCCGGTTAT TTATCACGGA CACACTCTTA CCACCAAGAT ACCATTTAAG 
GATGTGGTGG AAGCAATTAG CAGGTCTGCA TTTGTCAACT CCAACATGCC CGTCGTCCTG 
TCTATAGAGA ACCACTGCTC CCTCCCGCAG CAGCGCAAGA TGGCGGAAAT CTTCAAGACG 
GTGTTTGGGG AGCGTCTGGT GACACGCTTC CTCTTTGAAA GCGACTTCAG CGATGACCCT 
CACCTGCCAT CTCCACTGCA GCTGCAGGGG AGGATTCTCC TCAAGAATAA GAAGCTGAAA 
GCACACCAGG CTCCGGTGGA CATACTCAAG CAGAAGGCTC ACCAGCTTGC CCACATGCAG 
GCCCAGGTTA GCAATGGTTC ACCAGGGGTT TCCTCTCCCG GGACCCAAAC CAACGAGGAC 
GAGGAAGAGG AAGAAGACGA GTACGACTAT GACTATGAGT CTCTCTCAGA TGACAACCTC 
CTGGATGACA AACCTGAGGT GAAAAGCTCA GCTGACAAAG AGGAGCAGCC TGTGGATGAA 
ATACCCAAGA GAATGAAGAA GCCTGACAAC ACTACGCAGA GTAAAGGAAA GGTGTTTGAC 
ATGGAACTGG GCGAGGAGTT CTACCTTCCA CAGAACAAAA AGGAAAGTCG ACAGATCGCT 
CAGGAGCTGT CTGACCTCGT CATCTACTGT CAGGCCGTGA AGTTCCCTGG TCTGTCCACG 
CTGTCTCCGG CTGGCTCCGG CAGAGGGAAA GACCGTGGAA AGAGCAGGAA GTCCATATTT 
GGCACGGCTC CAGCTCGCTG CAGCGCAACA GGGGAGGTCA CCACTCCGAG TCGCACCCCT 
GGGAAAGGTG GCGCCGAGCG CCTGAGCTGG GAAGAGCAGC AAACGTCTCC TGTCCTCAGT 
CCACCCACCT CTCTCAGCGC CATTATCCGT ACACCCAAGT GTTATCACAT CTCCTCCGTC 
AATGAGAACG CCGCCAAGCG ACTGTGCCGG CGTTACTCTC AGAAACTGAT CCAGCACACC 
GTGTGTCAGC TGCTCAGGAC TTACCCAGCA GCCACCAGGA TCGACTCCAC AAACCCAAAC 
CCTCTGCTGT TCTGGCTGCA TGGCATTCAG CTGGTGGCTC TCAACTATCA GACTGACGAC 
CTGCCCATGC AGTTAAACAC AGCTCTGTTT GAGGCCAACG GCGGCTGCGG CTATGTCCTG 
AAACCAGCGG TGCTGTGGGA CCGAAACTGT CCGATGTATC AGCAGTTCTG TCCGATGGAG 
AGGGATGTGG AAAAAATGAG TCCTGCCGTC TACTCCCTTG CTATCGTTTC GGGGCAGAAC 
GTCTGTCCCG GTAACAGCAG CGGCAGCCCC TGCATCGAGG TGGACGTCCT GGGGATGCCG 
GTCGACAGCG CCCACTTCCG TACCAAACCC ATCCACCGCA ACACCCTCAA CCCCATGTGG 
AACGAGCACT TTCAGTTCAC CGTGCACTTT GAAGAGATGT GCTTCTTGCG CGTTGCTGTT 
GTGGAGAACA ACAGTTCCCA GACTACTGCT CAGAGAACTC TACCTCTGAA AGCCCTCAAG 
TCAGGTTACA GACACATCCA GCTGAGGACT CAACACAATG AATCTCTGGA AGTGTCAAGC 
TTGTTCATCT ATAGCCGAAG GACAGAGGAG TGTCCGACAG GGGGCGACAT TCCCTCATCC 
CTGCTGTTCA GCTCTGAGGA GAAGCCTGCA TCCCAGCAGC ACAGGGTGAC GGTGTACGGA 
GCTCCTGGTC CTGAGCCCTT CACTGTGTTC AGTGTGACGG AGCAGATCAC TGCCAAACAG 
CTGCTGGACA TGGTCGTAGC TTCTGCTGGT GGAAATCCCT ACTTCCTGTG TGAGGAGAAG 
GTTCCTTTGA CGAAGGAGCG CAGTGAGACA AAACGCTGTG CTCAGTATCG CCCCCTTGCT 
CCGGAGGAGG AGGTGGTCCG ACTGGTTTCC AGCTGGAGTG CCGAGGAGGG ATATGTGGGA 
AGGATCTGCT TCAAGCTCAG AGAGGAGAAA TTAAATGAGA AGAACGCAGC ACCAGAAGGA 
GAGGAGGAGT GGAGTGTGGG CGGAGCCAGG GAGGGAGCTG GTGGAGAAGG AGCAGCAGCA 
GCAGAGGATG ATGTGTTCCT GGTCCAGATC CATGAAGTTT CTCCAGAGCA GCCTCACACT 
GTGATCAAGG CCCCGCGCTA CAGCACAGCT CAGGACATCA TCCAGCAGAC GTTATTCAAG 
GCCAAGTATT CCTACAGCAT CCTCTCCAAT CCCAATCCAT GCGACTACGT CCTAATGGAG 
GAAGTAACCA AAGACGTGGG CTCAAAGAAG TCCTCCACCA CTAAGCCCCT GCAGCGGATG 
CTGCTGGATC ACGAGTGCGT CTATCAGGCT CAGAGCCGCT GGCGGGGCGC GGGGAAGTTC 
ATTCTGAAAC TGAAAGAGCA GTTGGTGCGG GAAGACAAAA AGAAAGTCAT TTCCTTTGCC 
AGTGAGCTGA AGAAACTGAC GAGCCGCGGC CGCAGCATGA CGACTGGCGG CGGCGTGGAC 
GGCCCCGGCC AGAGGTGTTG GTCTGCAGCA GTCGGTCTGA AGGTGTGGGT GGCAGATGGA 
AGGGTGGGGG CAGGTAGATT CCTTCCATCT TCCTCCTCTG GGAGACGTCT TCATCCAATC 
CACCTGCCCA CCTCCTCTCT GACTGCACCA GTGTTGAAGC TGCACCTCCT CAGGAACTGG 
AAGATCCACA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	plce1
Entrez Gene ID	101065955
Full Name	phospholipase C epsilon 1
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

plce1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム