depdc5 gene cDNA ORF clone, Takifugu rubripes(torafugu)

mRNA	Protein	Name
XM_011616322.1	XP_011614624.1	DEP domain-containing protein 5 isoform X1
XM_029831210.1	XP_029687070.1	GATOR complex protein DEPDC5 isoform X1
XM_011616322.2	XP_011614624.1	GATOR complex protein DEPDC5 isoform X2
XM_011616326.1	XP_011614628.1	DEP domain-containing protein 5 isoform X3
XM_011616327.1	XP_011614629.1	DEP domain-containing protein 5 isoform X4
XM_029831211.1	XP_029687071.1	GATOR complex protein DEPDC5 isoform X3
XM_029831212.1	XP_029687072.1	GATOR complex protein DEPDC5 isoform X4
XM_011616323.1	XP_011614625.1	DEP domain-containing protein 5 isoform X1
XM_011616324.1	XP_011614626.1	DEP domain-containing protein 5 isoform X2
XM_011616328.1	XP_011614630.1	DEP domain-containing protein 5 isoform X5
XM_029831213.1	XP_029687073.1	GATOR complex protein DEPDC5 isoform X5
XM_029831214.1	XP_029687074.1	GATOR complex protein DEPDC5 isoform X6
XM_029831216.1	XP_029687076.1	GATOR complex protein DEPDC5 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo62429	XM_029831216.1 Latest version!	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo21240	XM_011616323.1 Latest version!	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo21240	XM_011616322.2 Latest version!	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo21241	XM_011616324.1 Latest version!	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo21242	XM_011616326.1 Latest version!	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo21243	XM_011616327.1 Latest version!	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo21244	XM_011616328.1 Latest version!	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo62424	XM_029831210.1 Latest version!	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo62425	XM_029831211.1 Latest version!	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo62426	XM_029831212.1 Latest version!	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo62427	XM_029831213.1 Latest version!	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo62428	XM_029831214.1 Latest version!	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OTo21240	XM_011616322.1	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

depdc5 ( XM_029831216.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029687076.1 Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OTo62429
Clone ID Related Accession (Same CDS sequence)	XM_029831216.1
Accession Version	XM_029831216.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	GATOR complex protein DEPDC5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042306.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATTGC TGGTCTCTAT AAAGAAGCTT TTTGTTAAGT ATCCATTGCT GGTGCGGCTT 
GAAGAAACAG ATGGTGGCCC GGTTGGTTAT AACTCTACTG CAGCTCAAGG AAACTATCTG 
GAAGCCATTA ATCTTTCCTT CAATGTGTTT GACAAGCATT ACATCAACCG TAACTTTGAC 
CGCACTGGCC AGATGTCGGT GGTCATCACA CCTGGGGTTG GAGTGTTTGA AGTTGACCGC 
CAGCTCATGA TTCTTACCAA ACAGCGTATG ATTGATAATG GTATCGGGGT GGACTTGGTG 
TGTATGGGGG AACAGCCTCT ACATGCAGTA CCATTATTCA AGCTGCACAA CAGGATGATG 
GGTGTGGACT CTCATGTAGG AGATGATTAT CACCTTCCTC ACTGGATCAA CCACAGCTTC 
TATACCTCCA AAAGTCAAAC CTCTTGTAGC TCCTTCACCC CTCGAATCAC AGTGGCTGGA 
CTCAAGCTCA CTACAGAGAA GTCCAGATTC AACAAGGACC ACACTCTCTG TTCTCCAAAG 
GACACTGAAA GCAGTCTGCC TATACAGGTG GACTACGATG CCCATGATAT CCAGGTGTTC 
AGATTACCTG GTCCATCACG ATTTAAGAGG ACTCCTAATT TTAGGTTAGG GCGAGACAAA 
GAGATAGGTG GAAGAAAAAG TTTGGGCTCC CTGGAAGTGA CTGCATGCAT AGGGGTATCC 
CCCCCTGTTC GGTTTGAAGG TCCTGAAGAG CATAAAAGTG TAACATCAAA TGATAGTTTA 
GGCTCCGTGT CCAATGTGCT GCTCATTCCC CGTCTACCTT CAGCCCAGTA TGAAGTTAGC 
AGTTCCTTGG GATACACGAG CACCAGAGAA ATGTTAGAGA AGATGGAGTC ACAGAGGGAC 
TCCAGTGCAC CAGGAAGATT TACAGTGGGG AGTGCTGAGT CCCCAAGGCA CATCCGTCCT 
GGATGCTACA CCCCCCAAAG AGCACTCATC AATCCTTTCA CCCCATCAAG GATGCCTATG 
AAGCTAACCT CCAACCGCCG GCGTTGGATG CACACCTTCC CCATAGGTCC CTCTGGAGAG 
GCAATTCAAA TCCATCATCA GATGAGACAG AACATGGCTG ACCTGCAAGG CCAACAGACA 
AATCCTGCAC ATAGCTCAGC TGAGCTGTTG GAACTCGTCT GTCCTGAGGC CACTGGAAGA 
AAAACACTCT CAGGACAAGT GGGAGAAACT GGCCTTTATG TTGGGGGATT GATGGAAGAA 
TTGACTGCAA ATCCAGAGAG TAATGGTGGA CTTCCAACCC ACTGTGCCTC CTTATTTGAT 
GACTGTCCCC TCAGCAGTGC TGACCCAACC CTCCTGCTGT TTGCACCCTT GACAGTGCCC 
AGCTTTTGTT GTACAGTGGG GGTGGATTGG AAGTCTTTGA CGACACCAGC TTGTCTGCCC 
CTCACCACGG ACTATTTTCC AGATCGCCAA ACCCTTCAGA ATGATTATAC TGAAGGCTGC 
TATGACCTGC TACCACACAG TGACCTGGAC AGGCGGGAAG AAGAAGCAAC TGTAAGAACA 
GCTTCCGAAG TGTTTGAGGA GTTTATCTGT CAGAGACTGA TGCAGGGGTA TCAAATCATT 
GTTCAGACTC ATAACAGGAA GCCTCAGCCA TCTGTGTCCA CACCTCTTGG CAGCAGCCCT 
CTTTACTCCA GAGGCTTGGT TTCCCTGCGT CGAGCAGAAG AGAAGGAGAA TGTTTATTGG 
CTCAGCATGG GCCGTACCTT CCATAAAGTT TGCCTCAAAG ACAAAATAAT CAGTGTAACT 
CGCTATTTGC CAAAGTACCC GTGTGAGACT GCTCAGATCC AGTACAGTTA CAGCCTTTGT 
CCATCACACT CAGAGGGTCA ATTTGTGTCC TGTTGGGTGG AGTTTGGTCA CGAACGGCTG 
GAAGAATACA AGTGGAACTA TCTGGACCAG TACATCTGCT CTGCTGGGTC TGAGGACTTT 
AGTTTGATAG ACTCTCTAAA GTTTTGGAGG ACTCGGTTCT TGCTTCTGCC TGCTGGGGGA 
GCAAGGCGAA TGGCAGATGG TGAAGGGCAC TGGGATATTT ATGGAGAGGC AGTGGGTACT 
GCAATGGGCG GATCTGGGGA CTGGGTCTTG CTGGACAACT TCATCCGCTT CCTGGAGGGC 
CTGAACCGCA TTCGTCGCCG ACATCGCTCG GACAGGATCA TTAGACAAAA GGGGACACCA 
ATGAAAGGCC TGCAGGTTGC TGGTACTCTC CCTTCTTATT CTTCTGAACA CATGGCTCCT 
CCTCAAGGCA AAAAGGGCAC ATCTGCATTA TCAGTCTTAC TGGAGATGGA GCAGAAGACA 
CTGGATGAAC AGCAGGGAAC AAAGCCCTCA ACAGCTGTTG CTGACCCCTC TGGTATTGCA 
ACAAATCTCA TGTATCTGGA CAGTCCTCGC AAGGATGGTG GCTTCATTTT GGAATTTATT 
CGTAGCCCTC ATTCTTCCAA CACCTATCAC TCTCAGTTGC CTGTTGAAGC CAGTGAAGCG 
GCGGACAGAG GACTCCAGCC AACAGTTGCA CCTGGAGCAG CAGTCCAGCC TGTAGGAGAG 
CTTGCATCTT GTAGCAACAC AGGAGACACC AGTGGAAATT CTGCAGTGCA GTCGCTTTCT 
TTGTCATCAT CCCCAACACT CATGGAGATC CTGGAGGCTA TAAAACATCC AATGACAGGT 
GTACAACTCC TTCCAGAGCA AAGAGGCCTG CCTCTCAATT GCTTTTTTAG TGCGGAGGTG 
GTTCACTGGC TGATTAACAA CGTGGAAGGG GTAACGACAC AAGGCATAGC TGTAGATATC 
ATGCAGAAAA TGTTGGATGA AGGTTTGGTG GCCCATGCTT CTGGAGACAT GATGCGTACC 
TTCATTTATG GATTCTACTG CTACAGGATT GTTGGAGAGA AGGATGGTTC AAATTCCCAG 
CCTCCTTCCA CAACAGCAGG GGGCTGGCCT GCAGCAGCTC TGGAGGACTT TGCCCTGTTC 
CAGGGAAAGT GGTTTGAGGT GGCCTTTGTG GTGGAAGAGC AGCTCCATTG CGATCTCCCA 
GTTTTTCTCC TGCCCTGGCT CCCCAGCAGG CCGTCCTCCT ATGCAAGTAG GCATAGCTCA 
TTCAGCCGCA GTTTTGGAGG ACGCAGTCAG GCCGCTGCAT TGCTAGCTGC CACTGTTCCA 
GAGCAGAAAA TGGTCACTCT GGATGTGGAT GTGAACAATC GCAGTGACCG GATGGAGTGG 
TGCAGCTGTT ATTACCATGG CAACTTCTTC CTCAACACTG CCTTTGAGAT CAAGCTTCAC 
TGGATGGCTG TCACTGCTGC AGTACTCTTT GAGATGGTTC AGGGCTGGCA CAGGAAAGCA 
GCATCATGTG GCTTCCTCTT GATCCCTGCA CTGGAGTTTC CTTTTGCACT AACCTCCTCC 
CTGTATGGAG ATCCCCTCAG AGCCCAGCAC TTCATTTCTC TGAACATCCA CTGCCTTCTA 
AAGAATGGCA GTGACAATCT GTTTGAAGGC TTTGAACCAG AGACCTACTG GGATAGGATG 
CAGTTCTTTC AGGAGGCCAT ACTTTACAGG TTTGGATTTG TGCATGACAA ATTTTCAGTT 
TCAACCTTTA GTTTCCCTTC GGAGAATAAG CCCCAGTACA TCCACATAAC AGGTACCGTC 
TTCTTGCAGC TGCCATACTT CAAGAGGAAG CATTCAAGTG GGCAGCAACG TCGACGCCGG 
AACTCTACCA CTTCGAACAA CCAGGGGCTG TTTGGTGGGG AGGAGTGTGT TGGCTACTAT 
TGGGCCTACA ACACTATGCT GACCAAGTCC TGGAGGGCAG GCGTGCTTGG GGATGAGAAG 
CTCGCCGACC GCCTCCTCAG AGACTTCAGC GACTTCTGTG CCAACAAAGA CAACAGACTG 
TTAAACTTCT GGGACAGCTG TCAGGAGAAA ATGAACACCA GCACTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029687076.1
CDS	125..4075
Translation	MSLLVSIKKLFVKYPLLVRLEETDGGPVGYNSTAAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRQLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRMMGVDSHVGDDYHLPHWINHSFYTSKSQTSCSSFTPRITVAGLKLTTEKSRFNKDHTLCSPKDTESSLPIQVDYDAHDIQVFRLPGPSRFKRTPNFRLGRDKEIGGRKSLGSLEVTACIGVSPPVRFEGPEEHKSVTSNDSLGSVSNVLLIPRLPSAQYEVSSSLGYTSTREMLEKMESQRDSSAPGRFTVGSAESPRHIRPGCYTPQRALINPFTPSRMPMKLTSNRRRWMHTFPIGPSGEAIQIHHQMRQNMADLQGQQTNPAHSSAELLELVCPEATGRKTLSGQVGETGLYVGGLMEELTANPESNGGLPTHCASLFDDCPLSSADPTLLLFAPLTVPSFCCTVGVDWKSLTTPACLPLTTDYFPDRQTLQNDYTEGCYDLLPHSDLDRREEEATVRTASEVFEEFICQRLMQGYQIIVQTHNRKPQPSVSTPLGSSPLYSRGLVSLRRAEEKENVYWLSMGRTFHKVCLKDKIISVTRYLPKYPCETAQIQYSYSLCPSHSEGQFVSCWVEFGHERLEEYKWNYLDQYICSAGSEDFSLIDSLKFWRTRFLLLPAGGARRMADGEGHWDIYGEAVGTAMGGSGDWVLLDNFIRFLEGLNRIRRRHRSDRIIRQKGTPMKGLQVAGTLPSYSSEHMAPPQGKKGTSALSVLLEMEQKTLDEQQGTKPSTAVADPSGIATNLMYLDSPRKDGGFILEFIRSPHSSNTYHSQLPVEASEAADRGLQPTVAPGAAVQPVGELASCSNTGDTSGNSAVQSLSLSSSPTLMEILEAIKHPMTGVQLLPEQRGLPLNCFFSAEVVHWLINNVEGVTTQGIAVDIMQKMLDEGLVAHASGDMMRTFIYGFYCYRIVGEKDGSNSQPPSTTAGGWPAAALEDFALFQGKWFEVAFVVEEQLHCDLPVFLLPWLPSRPSSYASRHSSFSRSFGGRSQAAALLAATVPEQKMVTLDVDVNNRSDRMEWCSCYYHGNFFLNTAFEIKLHWMAVTAAVLFEMVQGWHRKAASCGFLLIPALEFPFALTSSLYGDPLRAQHFISLNIHCLLKNGSDNLFEGFEPETYWDRMQFFQEAILYRFGFVHDKFSVSTFSFPSENKPQYIHITGTVFLQLPYFKRKHSSGQQRRRRNSTTSNNQGLFGGEECVGYYWAYNTMLTKSWRAGVLGDEKLADRLLRDFSDFCANKDNRLLNFWDSCQEKMNTSTP

Target ORF information:

RefSeq Version	XM_029831216.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029831216.1

ORF Insert Sequence:

ATGTCATTGC TGGTCTCTAT AAAGAAGCTT TTTGTTAAGT ATCCATTGCT GGTGCGGCTT 
GAAGAAACAG ATGGTGGCCC GGTTGGTTAT AACTCTACTG CAGCTCAAGG AAACTATCTG 
GAAGCCATTA ATCTTTCCTT CAATGTGTTT GACAAGCATT ACATCAACCG TAACTTTGAC 
CGCACTGGCC AGATGTCGGT GGTCATCACA CCTGGGGTTG GAGTGTTTGA AGTTGACCGC 
CAGCTCATGA TTCTTACCAA ACAGCGTATG ATTGATAATG GTATCGGGGT GGACTTGGTG 
TGTATGGGGG AACAGCCTCT ACATGCAGTA CCATTATTCA AGCTGCACAA CAGGATGATG 
GGTGTGGACT CTCATGTAGG AGATGATTAT CACCTTCCTC ACTGGATCAA CCACAGCTTC 
TATACCTCCA AAAGTCAAAC CTCTTGTAGC TCCTTCACCC CTCGAATCAC AGTGGCTGGA 
CTCAAGCTCA CTACAGAGAA GTCCAGATTC AACAAGGACC ACACTCTCTG TTCTCCAAAG 
GACACTGAAA GCAGTCTGCC TATACAGGTG GACTACGATG CCCATGATAT CCAGGTGTTC 
AGATTACCTG GTCCATCACG ATTTAAGAGG ACTCCTAATT TTAGGTTAGG GCGAGACAAA 
GAGATAGGTG GAAGAAAAAG TTTGGGCTCC CTGGAAGTGA CTGCATGCAT AGGGGTATCC 
CCCCCTGTTC GGTTTGAAGG TCCTGAAGAG CATAAAAGTG TAACATCAAA TGATAGTTTA 
GGCTCCGTGT CCAATGTGCT GCTCATTCCC CGTCTACCTT CAGCCCAGTA TGAAGTTAGC 
AGTTCCTTGG GATACACGAG CACCAGAGAA ATGTTAGAGA AGATGGAGTC ACAGAGGGAC 
TCCAGTGCAC CAGGAAGATT TACAGTGGGG AGTGCTGAGT CCCCAAGGCA CATCCGTCCT 
GGATGCTACA CCCCCCAAAG AGCACTCATC AATCCTTTCA CCCCATCAAG GATGCCTATG 
AAGCTAACCT CCAACCGCCG GCGTTGGATG CACACCTTCC CCATAGGTCC CTCTGGAGAG 
GCAATTCAAA TCCATCATCA GATGAGACAG AACATGGCTG ACCTGCAAGG CCAACAGACA 
AATCCTGCAC ATAGCTCAGC TGAGCTGTTG GAACTCGTCT GTCCTGAGGC CACTGGAAGA 
AAAACACTCT CAGGACAAGT GGGAGAAACT GGCCTTTATG TTGGGGGATT GATGGAAGAA 
TTGACTGCAA ATCCAGAGAG TAATGGTGGA CTTCCAACCC ACTGTGCCTC CTTATTTGAT 
GACTGTCCCC TCAGCAGTGC TGACCCAACC CTCCTGCTGT TTGCACCCTT GACAGTGCCC 
AGCTTTTGTT GTACAGTGGG GGTGGATTGG AAGTCTTTGA CGACACCAGC TTGTCTGCCC 
CTCACCACGG ACTATTTTCC AGATCGCCAA ACCCTTCAGA ATGATTATAC TGAAGGCTGC 
TATGACCTGC TACCACACAG TGACCTGGAC AGGCGGGAAG AAGAAGCAAC TGTAAGAACA 
GCTTCCGAAG TGTTTGAGGA GTTTATCTGT CAGAGACTGA TGCAGGGGTA TCAAATCATT 
GTTCAGACTC ATAACAGGAA GCCTCAGCCA TCTGTGTCCA CACCTCTTGG CAGCAGCCCT 
CTTTACTCCA GAGGCTTGGT TTCCCTGCGT CGAGCAGAAG AGAAGGAGAA TGTTTATTGG 
CTCAGCATGG GCCGTACCTT CCATAAAGTT TGCCTCAAAG ACAAAATAAT CAGTGTAACT 
CGCTATTTGC CAAAGTACCC GTGTGAGACT GCTCAGATCC AGTACAGTTA CAGCCTTTGT 
CCATCACACT CAGAGGGTCA ATTTGTGTCC TGTTGGGTGG AGTTTGGTCA CGAACGGCTG 
GAAGAATACA AGTGGAACTA TCTGGACCAG TACATCTGCT CTGCTGGGTC TGAGGACTTT 
AGTTTGATAG ACTCTCTAAA GTTTTGGAGG ACTCGGTTCT TGCTTCTGCC TGCTGGGGGA 
GCAAGGCGAA TGGCAGATGG TGAAGGGCAC TGGGATATTT ATGGAGAGGC AGTGGGTACT 
GCAATGGGCG GATCTGGGGA CTGGGTCTTG CTGGACAACT TCATCCGCTT CCTGGAGGGC 
CTGAACCGCA TTCGTCGCCG ACATCGCTCG GACAGGATCA TTAGACAAAA GGGGACACCA 
ATGAAAGGCC TGCAGGTTGC TGGTACTCTC CCTTCTTATT CTTCTGAACA CATGGCTCCT 
CCTCAAGGCA AAAAGGGCAC ATCTGCATTA TCAGTCTTAC TGGAGATGGA GCAGAAGACA 
CTGGATGAAC AGCAGGGAAC AAAGCCCTCA ACAGCTGTTG CTGACCCCTC TGGTATTGCA 
ACAAATCTCA TGTATCTGGA CAGTCCTCGC AAGGATGGTG GCTTCATTTT GGAATTTATT 
CGTAGCCCTC ATTCTTCCAA CACCTATCAC TCTCAGTTGC CTGTTGAAGC CAGTGAAGCG 
GCGGACAGAG GACTCCAGCC AACAGTTGCA CCTGGAGCAG CAGTCCAGCC TGTAGGAGAG 
CTTGCATCTT GTAGCAACAC AGGAGACACC AGTGGAAATT CTGCAGTGCA GTCGCTTTCT 
TTGTCATCAT CCCCAACACT CATGGAGATC CTGGAGGCTA TAAAACATCC AATGACAGGT 
GTACAACTCC TTCCAGAGCA AAGAGGCCTG CCTCTCAATT GCTTTTTTAG TGCGGAGGTG 
GTTCACTGGC TGATTAACAA CGTGGAAGGG GTAACGACAC AAGGCATAGC TGTAGATATC 
ATGCAGAAAA TGTTGGATGA AGGTTTGGTG GCCCATGCTT CTGGAGACAT GATGCGTACC 
TTCATTTATG GATTCTACTG CTACAGGATT GTTGGAGAGA AGGATGGTTC AAATTCCCAG 
CCTCCTTCCA CAACAGCAGG GGGCTGGCCT GCAGCAGCTC TGGAGGACTT TGCCCTGTTC 
CAGGGAAAGT GGTTTGAGGT GGCCTTTGTG GTGGAAGAGC AGCTCCATTG CGATCTCCCA 
GTTTTTCTCC TGCCCTGGCT CCCCAGCAGG CCGTCCTCCT ATGCAAGTAG GCATAGCTCA 
TTCAGCCGCA GTTTTGGAGG ACGCAGTCAG GCCGCTGCAT TGCTAGCTGC CACTGTTCCA 
GAGCAGAAAA TGGTCACTCT GGATGTGGAT GTGAACAATC GCAGTGACCG GATGGAGTGG 
TGCAGCTGTT ATTACCATGG CAACTTCTTC CTCAACACTG CCTTTGAGAT CAAGCTTCAC 
TGGATGGCTG TCACTGCTGC AGTACTCTTT GAGATGGTTC AGGGCTGGCA CAGGAAAGCA 
GCATCATGTG GCTTCCTCTT GATCCCTGCA CTGGAGTTTC CTTTTGCACT AACCTCCTCC 
CTGTATGGAG ATCCCCTCAG AGCCCAGCAC TTCATTTCTC TGAACATCCA CTGCCTTCTA 
AAGAATGGCA GTGACAATCT GTTTGAAGGC TTTGAACCAG AGACCTACTG GGATAGGATG 
CAGTTCTTTC AGGAGGCCAT ACTTTACAGG TTTGGATTTG TGCATGACAA ATTTTCAGTT 
TCAACCTTTA GTTTCCCTTC GGAGAATAAG CCCCAGTACA TCCACATAAC AGGTACCGTC 
TTCTTGCAGC TGCCATACTT CAAGAGGAAG CATTCAAGTG GGCAGCAACG TCGACGCCGG 
AACTCTACCA CTTCGAACAA CCAGGGGCTG TTTGGTGGGG AGGAGTGTGT TGGCTACTAT 
TGGGCCTACA ACACTATGCT GACCAAGTCC TGGAGGGCAG GCGTGCTTGG GGATGAGAAG 
CTCGCCGACC GCCTCCTCAG AGACTTCAGC GACTTCTGTG CCAACAAAGA CAACAGACTG 
TTAAACTTCT GGGACAGCTG TCAGGAGAAA ATGAACACCA GCACTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

depdc5 ( XM_011616322.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011614624.1 Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo21240
Clone ID Related Accession (Same CDS sequence)	XM_011616322.1 , XM_011616322.2 , XM_011616323.1
Accession Version	XM_011616322.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	DEP domain-containing protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071163.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG GTCCTGAAGA GCATAAAAGT 
GTAACATCAA ATGATAGTTT AGGCTCCGTG TCCAATGTGC TGCTCATTCC CCGTCTACCT 
TCAGCCCAGT ATGAAGTTAG CAGTTCCTTG GGATACACGA GCACCAGAGA AATGTTAGAG 
AAGATGGAGT CACAGAGGGA CTCCAGTGCA CCAGGAAGAT TTACAGTGGG GAGTGCTGAG 
TCCCCAAGGC ACATCCGTCC TGGATGCTAC ACCCCCCAAA GAGCACTCAT CAATCCTTTC 
ACCCCATCAA GGATGCCTAT GAAGCTAACC TCCAACCGCC GGCGTTGGAT GCACACCTTC 
CCCATAGGTC CCTCTGGAGA GGCAATTCAA ATCCATCATC AGATGAGACA GAACATGGCT 
GACCTGCAAG GCCAACAGAC AAATCCTGCA CATAGCTCAG CTGAGCTGTT GGAACTCGTC 
TGTCCTGAGG CCACTGGAAG AAAAACACTC TCAGGACAAG TGGGAGAAAC TGGCCTTTAT 
GTTGGGGGAT TGATGGAAGA ATTGACTGCA AATCCAGAGA GTAATGGTGG ACTTCCAACC 
CACTGTGCCT CCTTATTTGA TGACTGTCCC CTCAGCAGTG CTGACCCAAT GCCCAGCTTT 
TGTTGTACAG TGGGGGTGGA TTGGAAGTCT TTGACGACAC CAGCTTGTCT GCCCCTCACC 
ACGGACTATT TTCCAGATCG CCAAACCCTT CAGAATGATT ATACTGAAGG CTGCTATGAC 
CTGCTACCAC ACAGTGACCT GGACAGGCGG GAAGAAGAAG CAACTGTAAG AACAGCTTCC 
GAAGTGTTTG AGGAGTTTAT CTGTCAGAGA CTGATGCAGG GGTATCAAAT CATTGTTCAG 
ACTCATAACA GGAAGCCTCA GCCATCTGTG TCCACACCTC TTGGCAGCAG CCCTCTTTAC 
TCCAGAGGCT TGGTTTCCCT GCGTCGAGCA GAAGAGAAGG AGAATGTTTA TTGGCTCAGC 
ATGGGCCGTA CCTTCCATAA AGTTTGCCTC AAAGACAAAA TAATCAGTGT AACTCGCTAT 
TTGCCAAAGT ACCCGTGTGA GACTGCTCAG ATCCAGTACA GTTACAGCCT TTGTCCATCA 
CACTCAGAGG GTCAATTTGT GTCCTGTTGG GTGGAGTTTG GTCACGAACG GCTGGAAGAA 
TACAAGTGGA ACTATCTGGA CCAGTACATC TGCTCTGCTG GGTCTGAGGA CTTTAGTTTG 
ATAGACTCTC TAAAGTTTTG GAGGACTCGG TTCTTGCTTC TGCCTGCTGG GGGAGCAAGG 
CGAATGGCAG ATGGTGAAGG GCACTGGGAT ATTTATGGAG AGGCAGTGGG TACTGCAATG 
GGCGGATCTG GGGACTGGGT CTTGCTGGAC AACTTCATCC GCTTCCTGGA GGGCCTGAAC 
CGCATTCGTC GCCGACATCG CTCGGACAGG ATCATTAGAC AAAAGGGGAC ACCAATGAAA 
GGCCTGCAGG TTGCTGGTAC TCTCCCTTCT TATTCTTCTG AACACATGGC TCCTCCTCAA 
GGCAAAAAGG GCACATCTGC ATTATCAGTC TTACTGGAGA TGGAGCAGAA GACACTGGAT 
GAACAGCAGG GAACAAAGCC CTCAACAGCT GTTGCTGACC CCTCTGGTAT TGCAACAAAT 
CTCATGTATC TGGACAGTCC TCGCAAGGAT GGTGGCTTCA TTTTGGAATT TATTCGTAGC 
CCTCATTCTT CCAACACCTA TCACTCTCAG TTGCCTGTTG AAGCCAGTGA AGCGGCGGAC 
AGAGGACTCC AGCCAACAGT TGCACCTGGA GCAGCAGTCC AGCCTGTAGG AGAGCTTGCA 
TCTTGTAGCA ACACAGGAGA CACCAGTGGA AATTCTGCAG TGCAGTCGCT TTCTTTGTCA 
TCATCCCCAA CACTCATGGA GATCCTGGAG GCTATAAAAC ATCCAATGAC AGGTGTACAA 
CTCCTTCCAG AGCAAAGAGG CCTGCCTCTC AATTGCTTTT TTAGTGCGGA GGTGGTTCAC 
TGGCTGATTA ACAACGTGGA AGGGGTAACG ACACAAGGCA TAGCTGTAGA TATCATGCAG 
AAAATGTTGG ATGAAGGTTT GGTGGCCCAT GCTTCTGGAG ACATGATGCG TACCTTCATT 
TATGGATTCT ACTGCTACAG GATTGTTGGA GAGAAGGATG GTTCAAATTC CCAGCCTCCT 
TCCACAACAG CAGGGGGCTG GCCTGCAGCA GCTCTGGAGG ACTTTGCCCT GTTCCAGGGA 
AAGTGGTTTG AGGTGGCCTT TGTGGTGGAA GAGCAGCTCC ATTGCGATCT CCCAGTTTTT 
CTCCTGCCCT GGCTCCCCAG CAGGCCGTCC TCCTATGCAA GTAGGCATAG CTCATTCAGC 
CGCAGTTTTG GAGGACGCAG TCAGGCCGCT GCATTGCTAG CTGCCACTGT TCCAGAGCAG 
AAAATGGTCA CTCTGGATGT GGATGTGAAC AATCGCAGTG ACCGGATGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT CTTCCTCAAC ACTGCCTTTG AGATCAAGCT TCACTGGATG 
GCTGTCACTG CTGCAGTACT CTTTGAGATG GTTCAGGGCT GGCACAGGAA AGCAGCATCA 
TGTGGCTTCC TCTTGATCCC TGCACTGGAG TTTCCTTTTG CACTAACCTC CTCCCTGTAT 
GGAGATCCCC TCAGAGCCCA GCACTTCATT TCTCTGAACA TCCACTGCCT TCTAAAGAAT 
GGCAGTGACA ATCTGTTTGA AGGCTTTGAA CCAGAGACCT ACTGGGATAG GATGCAGTTC 
TTTCAGGAGG CCATACTTTA CAGGTTTGGA TTTGTGCATG ACAAATTTTC AGTTTCAACC 
TTTAGTTTCC CTTCGGAGAA TAAGCCCCAG TACATCCACA TAACAGGTAC CGTCTTCTTG 
CAGCTGCCAT ACTTCAAGAG GAAGCATTCA AGTGGGCAGC AACGTCGACG CCGGAACTCT 
ACCACTTCGA ACAACCAGGG GCTGTTTGGT GGGGAGGAGT GTGTTGGCTA CTATTGGGCC 
TACAACACTA TGCTGACCAA GTCCTGGAGG GCAGGCGTGC TTGGGGATGA GAAGCTCGCC 
GACCGCCTCC TCAGAGACTT CAGCGACTTC TGTGCCAACA AAGACAACAG ACTGTTAAAC 
TTCTGGGACA GCTGTCAGGA GAAAATGAAC ACCAGCACTC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011614624.1
CDS	207..4931
Translation	MRTNKSYKLVLHKKGFGGSDDELVVNPKVFPQVCLRDIIEIAHPTDEYSPLLLQVKNLKEDLQKETISVDQTVAQAFKLRAYQDVIVNVINPKDVTLDLVELTFKDQYIGRGDMWRLKKCLVSTCAYVTQKVEFAGIRAQASELWLKGEKVTCGYISEDTRVVFRSTSAMVYIFIQMSSEMWDFDIYGDLYFEKAVNGFLSDLFAKWKEKNCSHEVTVVLFSRTFYNAKTVDEFPEVLRGSIRQDQECRFFEDFYRVVAQNERRDEWMSLLVSIKKLFVKYPLLVRLEETDGGPVGYNSTAAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRQLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRMMGVDSHVGDDYHLPHWINHSFYTSKSQTSCSSFTPRITVAGLKLTTEKSRFNKDHTLCSPKDTESSLPIQVDYDAHDIQVFRLPGPSRFKRTPNFRLGRDKEIGGRKSLGSLEVTACIGVSPPVRFEGPEEHKSVTSNDSLGSVSNVLLIPRLPSAQYEVSSSLGYTSTREMLEKMESQRDSSAPGRFTVGSAESPRHIRPGCYTPQRALINPFTPSRMPMKLTSNRRRWMHTFPIGPSGEAIQIHHQMRQNMADLQGQQTNPAHSSAELLELVCPEATGRKTLSGQVGETGLYVGGLMEELTANPESNGGLPTHCASLFDDCPLSSADPMPSFCCTVGVDWKSLTTPACLPLTTDYFPDRQTLQNDYTEGCYDLLPHSDLDRREEEATVRTASEVFEEFICQRLMQGYQIIVQTHNRKPQPSVSTPLGSSPLYSRGLVSLRRAEEKENVYWLSMGRTFHKVCLKDKIISVTRYLPKYPCETAQIQYSYSLCPSHSEGQFVSCWVEFGHERLEEYKWNYLDQYICSAGSEDFSLIDSLKFWRTRFLLLPAGGARRMADGEGHWDIYGEAVGTAMGGSGDWVLLDNFIRFLEGLNRIRRRHRSDRIIRQKGTPMKGLQVAGTLPSYSSEHMAPPQGKKGTSALSVLLEMEQKTLDEQQGTKPSTAVADPSGIATNLMYLDSPRKDGGFILEFIRSPHSSNTYHSQLPVEASEAADRGLQPTVAPGAAVQPVGELASCSNTGDTSGNSAVQSLSLSSSPTLMEILEAIKHPMTGVQLLPEQRGLPLNCFFSAEVVHWLINNVEGVTTQGIAVDIMQKMLDEGLVAHASGDMMRTFIYGFYCYRIVGEKDGSNSQPPSTTAGGWPAAALEDFALFQGKWFEVAFVVEEQLHCDLPVFLLPWLPSRPSSYASRHSSFSRSFGGRSQAAALLAATVPEQKMVTLDVDVNNRSDRMEWCSCYYHGNFFLNTAFEIKLHWMAVTAAVLFEMVQGWHRKAASCGFLLIPALEFPFALTSSLYGDPLRAQHFISLNIHCLLKNGSDNLFEGFEPETYWDRMQFFQEAILYRFGFVHDKFSVSTFSFPSENKPQYIHITGTVFLQLPYFKRKHSSGQQRRRRNSTTSNNQGLFGGEECVGYYWAYNTMLTKSWRAGVLGDEKLADRLLRDFSDFCANKDNRLLNFWDSCQEKMNTSTP

Target ORF information:

RefSeq Version	XM_011616322.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011616322.1

ORF Insert Sequence:

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG GTCCTGAAGA GCATAAAAGT 
GTAACATCAA ATGATAGTTT AGGCTCCGTG TCCAATGTGC TGCTCATTCC CCGTCTACCT 
TCAGCCCAGT ATGAAGTTAG CAGTTCCTTG GGATACACGA GCACCAGAGA AATGTTAGAG 
AAGATGGAGT CACAGAGGGA CTCCAGTGCA CCAGGAAGAT TTACAGTGGG GAGTGCTGAG 
TCCCCAAGGC ACATCCGTCC TGGATGCTAC ACCCCCCAAA GAGCACTCAT CAATCCTTTC 
ACCCCATCAA GGATGCCTAT GAAGCTAACC TCCAACCGCC GGCGTTGGAT GCACACCTTC 
CCCATAGGTC CCTCTGGAGA GGCAATTCAA ATCCATCATC AGATGAGACA GAACATGGCT 
GACCTGCAAG GCCAACAGAC AAATCCTGCA CATAGCTCAG CTGAGCTGTT GGAACTCGTC 
TGTCCTGAGG CCACTGGAAG AAAAACACTC TCAGGACAAG TGGGAGAAAC TGGCCTTTAT 
GTTGGGGGAT TGATGGAAGA ATTGACTGCA AATCCAGAGA GTAATGGTGG ACTTCCAACC 
CACTGTGCCT CCTTATTTGA TGACTGTCCC CTCAGCAGTG CTGACCCAAT GCCCAGCTTT 
TGTTGTACAG TGGGGGTGGA TTGGAAGTCT TTGACGACAC CAGCTTGTCT GCCCCTCACC 
ACGGACTATT TTCCAGATCG CCAAACCCTT CAGAATGATT ATACTGAAGG CTGCTATGAC 
CTGCTACCAC ACAGTGACCT GGACAGGCGG GAAGAAGAAG CAACTGTAAG AACAGCTTCC 
GAAGTGTTTG AGGAGTTTAT CTGTCAGAGA CTGATGCAGG GGTATCAAAT CATTGTTCAG 
ACTCATAACA GGAAGCCTCA GCCATCTGTG TCCACACCTC TTGGCAGCAG CCCTCTTTAC 
TCCAGAGGCT TGGTTTCCCT GCGTCGAGCA GAAGAGAAGG AGAATGTTTA TTGGCTCAGC 
ATGGGCCGTA CCTTCCATAA AGTTTGCCTC AAAGACAAAA TAATCAGTGT AACTCGCTAT 
TTGCCAAAGT ACCCGTGTGA GACTGCTCAG ATCCAGTACA GTTACAGCCT TTGTCCATCA 
CACTCAGAGG GTCAATTTGT GTCCTGTTGG GTGGAGTTTG GTCACGAACG GCTGGAAGAA 
TACAAGTGGA ACTATCTGGA CCAGTACATC TGCTCTGCTG GGTCTGAGGA CTTTAGTTTG 
ATAGACTCTC TAAAGTTTTG GAGGACTCGG TTCTTGCTTC TGCCTGCTGG GGGAGCAAGG 
CGAATGGCAG ATGGTGAAGG GCACTGGGAT ATTTATGGAG AGGCAGTGGG TACTGCAATG 
GGCGGATCTG GGGACTGGGT CTTGCTGGAC AACTTCATCC GCTTCCTGGA GGGCCTGAAC 
CGCATTCGTC GCCGACATCG CTCGGACAGG ATCATTAGAC AAAAGGGGAC ACCAATGAAA 
GGCCTGCAGG TTGCTGGTAC TCTCCCTTCT TATTCTTCTG AACACATGGC TCCTCCTCAA 
GGCAAAAAGG GCACATCTGC ATTATCAGTC TTACTGGAGA TGGAGCAGAA GACACTGGAT 
GAACAGCAGG GAACAAAGCC CTCAACAGCT GTTGCTGACC CCTCTGGTAT TGCAACAAAT 
CTCATGTATC TGGACAGTCC TCGCAAGGAT GGTGGCTTCA TTTTGGAATT TATTCGTAGC 
CCTCATTCTT CCAACACCTA TCACTCTCAG TTGCCTGTTG AAGCCAGTGA AGCGGCGGAC 
AGAGGACTCC AGCCAACAGT TGCACCTGGA GCAGCAGTCC AGCCTGTAGG AGAGCTTGCA 
TCTTGTAGCA ACACAGGAGA CACCAGTGGA AATTCTGCAG TGCAGTCGCT TTCTTTGTCA 
TCATCCCCAA CACTCATGGA GATCCTGGAG GCTATAAAAC ATCCAATGAC AGGTGTACAA 
CTCCTTCCAG AGCAAAGAGG CCTGCCTCTC AATTGCTTTT TTAGTGCGGA GGTGGTTCAC 
TGGCTGATTA ACAACGTGGA AGGGGTAACG ACACAAGGCA TAGCTGTAGA TATCATGCAG 
AAAATGTTGG ATGAAGGTTT GGTGGCCCAT GCTTCTGGAG ACATGATGCG TACCTTCATT 
TATGGATTCT ACTGCTACAG GATTGTTGGA GAGAAGGATG GTTCAAATTC CCAGCCTCCT 
TCCACAACAG CAGGGGGCTG GCCTGCAGCA GCTCTGGAGG ACTTTGCCCT GTTCCAGGGA 
AAGTGGTTTG AGGTGGCCTT TGTGGTGGAA GAGCAGCTCC ATTGCGATCT CCCAGTTTTT 
CTCCTGCCCT GGCTCCCCAG CAGGCCGTCC TCCTATGCAA GTAGGCATAG CTCATTCAGC 
CGCAGTTTTG GAGGACGCAG TCAGGCCGCT GCATTGCTAG CTGCCACTGT TCCAGAGCAG 
AAAATGGTCA CTCTGGATGT GGATGTGAAC AATCGCAGTG ACCGGATGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT CTTCCTCAAC ACTGCCTTTG AGATCAAGCT TCACTGGATG 
GCTGTCACTG CTGCAGTACT CTTTGAGATG GTTCAGGGCT GGCACAGGAA AGCAGCATCA 
TGTGGCTTCC TCTTGATCCC TGCACTGGAG TTTCCTTTTG CACTAACCTC CTCCCTGTAT 
GGAGATCCCC TCAGAGCCCA GCACTTCATT TCTCTGAACA TCCACTGCCT TCTAAAGAAT 
GGCAGTGACA ATCTGTTTGA AGGCTTTGAA CCAGAGACCT ACTGGGATAG GATGCAGTTC 
TTTCAGGAGG CCATACTTTA CAGGTTTGGA TTTGTGCATG ACAAATTTTC AGTTTCAACC 
TTTAGTTTCC CTTCGGAGAA TAAGCCCCAG TACATCCACA TAACAGGTAC CGTCTTCTTG 
CAGCTGCCAT ACTTCAAGAG GAAGCATTCA AGTGGGCAGC AACGTCGACG CCGGAACTCT 
ACCACTTCGA ACAACCAGGG GCTGTTTGGT GGGGAGGAGT GTGTTGGCTA CTATTGGGCC 
TACAACACTA TGCTGACCAA GTCCTGGAGG GCAGGCGTGC TTGGGGATGA GAAGCTCGCC 
GACCGCCTCC TCAGAGACTT CAGCGACTTC TGTGCCAACA AAGACAACAG ACTGTTAAAC 
TTCTGGGACA GCTGTCAGGA GAAAATGAAC ACCAGCACTC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

depdc5 ( XM_011616322.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011614624.1 Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X2, mRNA.

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CloneID	OTo21240
Clone ID Related Accession (Same CDS sequence)	XM_011616322.1 , XM_011616322.2 , XM_011616323.1
Accession Version	XM_011616322.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	GATOR complex protein DEPDC5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042306.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011616322.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG GTCCTGAAGA GCATAAAAGT 
GTAACATCAA ATGATAGTTT AGGCTCCGTG TCCAATGTGC TGCTCATTCC CCGTCTACCT 
TCAGCCCAGT ATGAAGTTAG CAGTTCCTTG GGATACACGA GCACCAGAGA AATGTTAGAG 
AAGATGGAGT CACAGAGGGA CTCCAGTGCA CCAGGAAGAT TTACAGTGGG GAGTGCTGAG 
TCCCCAAGGC ACATCCGTCC TGGATGCTAC ACCCCCCAAA GAGCACTCAT CAATCCTTTC 
ACCCCATCAA GGATGCCTAT GAAGCTAACC TCCAACCGCC GGCGTTGGAT GCACACCTTC 
CCCATAGGTC CCTCTGGAGA GGCAATTCAA ATCCATCATC AGATGAGACA GAACATGGCT 
GACCTGCAAG GCCAACAGAC AAATCCTGCA CATAGCTCAG CTGAGCTGTT GGAACTCGTC 
TGTCCTGAGG CCACTGGAAG AAAAACACTC TCAGGACAAG TGGGAGAAAC TGGCCTTTAT 
GTTGGGGGAT TGATGGAAGA ATTGACTGCA AATCCAGAGA GTAATGGTGG ACTTCCAACC 
CACTGTGCCT CCTTATTTGA TGACTGTCCC CTCAGCAGTG CTGACCCAAT GCCCAGCTTT 
TGTTGTACAG TGGGGGTGGA TTGGAAGTCT TTGACGACAC CAGCTTGTCT GCCCCTCACC 
ACGGACTATT TTCCAGATCG CCAAACCCTT CAGAATGATT ATACTGAAGG CTGCTATGAC 
CTGCTACCAC ACAGTGACCT GGACAGGCGG GAAGAAGAAG CAACTGTAAG AACAGCTTCC 
GAAGTGTTTG AGGAGTTTAT CTGTCAGAGA CTGATGCAGG GGTATCAAAT CATTGTTCAG 
ACTCATAACA GGAAGCCTCA GCCATCTGTG TCCACACCTC TTGGCAGCAG CCCTCTTTAC 
TCCAGAGGCT TGGTTTCCCT GCGTCGAGCA GAAGAGAAGG AGAATGTTTA TTGGCTCAGC 
ATGGGCCGTA CCTTCCATAA AGTTTGCCTC AAAGACAAAA TAATCAGTGT AACTCGCTAT 
TTGCCAAAGT ACCCGTGTGA GACTGCTCAG ATCCAGTACA GTTACAGCCT TTGTCCATCA 
CACTCAGAGG GTCAATTTGT GTCCTGTTGG GTGGAGTTTG GTCACGAACG GCTGGAAGAA 
TACAAGTGGA ACTATCTGGA CCAGTACATC TGCTCTGCTG GGTCTGAGGA CTTTAGTTTG 
ATAGACTCTC TAAAGTTTTG GAGGACTCGG TTCTTGCTTC TGCCTGCTGG GGGAGCAAGG 
CGAATGGCAG ATGGTGAAGG GCACTGGGAT ATTTATGGAG AGGCAGTGGG TACTGCAATG 
GGCGGATCTG GGGACTGGGT CTTGCTGGAC AACTTCATCC GCTTCCTGGA GGGCCTGAAC 
CGCATTCGTC GCCGACATCG CTCGGACAGG ATCATTAGAC AAAAGGGGAC ACCAATGAAA 
GGCCTGCAGG TTGCTGGTAC TCTCCCTTCT TATTCTTCTG AACACATGGC TCCTCCTCAA 
GGCAAAAAGG GCACATCTGC ATTATCAGTC TTACTGGAGA TGGAGCAGAA GACACTGGAT 
GAACAGCAGG GAACAAAGCC CTCAACAGCT GTTGCTGACC CCTCTGGTAT TGCAACAAAT 
CTCATGTATC TGGACAGTCC TCGCAAGGAT GGTGGCTTCA TTTTGGAATT TATTCGTAGC 
CCTCATTCTT CCAACACCTA TCACTCTCAG TTGCCTGTTG AAGCCAGTGA AGCGGCGGAC 
AGAGGACTCC AGCCAACAGT TGCACCTGGA GCAGCAGTCC AGCCTGTAGG AGAGCTTGCA 
TCTTGTAGCA ACACAGGAGA CACCAGTGGA AATTCTGCAG TGCAGTCGCT TTCTTTGTCA 
TCATCCCCAA CACTCATGGA GATCCTGGAG GCTATAAAAC ATCCAATGAC AGGTGTACAA 
CTCCTTCCAG AGCAAAGAGG CCTGCCTCTC AATTGCTTTT TTAGTGCGGA GGTGGTTCAC 
TGGCTGATTA ACAACGTGGA AGGGGTAACG ACACAAGGCA TAGCTGTAGA TATCATGCAG 
AAAATGTTGG ATGAAGGTTT GGTGGCCCAT GCTTCTGGAG ACATGATGCG TACCTTCATT 
TATGGATTCT ACTGCTACAG GATTGTTGGA GAGAAGGATG GTTCAAATTC CCAGCCTCCT 
TCCACAACAG CAGGGGGCTG GCCTGCAGCA GCTCTGGAGG ACTTTGCCCT GTTCCAGGGA 
AAGTGGTTTG AGGTGGCCTT TGTGGTGGAA GAGCAGCTCC ATTGCGATCT CCCAGTTTTT 
CTCCTGCCCT GGCTCCCCAG CAGGCCGTCC TCCTATGCAA GTAGGCATAG CTCATTCAGC 
CGCAGTTTTG GAGGACGCAG TCAGGCCGCT GCATTGCTAG CTGCCACTGT TCCAGAGCAG 
AAAATGGTCA CTCTGGATGT GGATGTGAAC AATCGCAGTG ACCGGATGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT CTTCCTCAAC ACTGCCTTTG AGATCAAGCT TCACTGGATG 
GCTGTCACTG CTGCAGTACT CTTTGAGATG GTTCAGGGCT GGCACAGGAA AGCAGCATCA 
TGTGGCTTCC TCTTGATCCC TGCACTGGAG TTTCCTTTTG CACTAACCTC CTCCCTGTAT 
GGAGATCCCC TCAGAGCCCA GCACTTCATT TCTCTGAACA TCCACTGCCT TCTAAAGAAT 
GGCAGTGACA ATCTGTTTGA AGGCTTTGAA CCAGAGACCT ACTGGGATAG GATGCAGTTC 
TTTCAGGAGG CCATACTTTA CAGGTTTGGA TTTGTGCATG ACAAATTTTC AGTTTCAACC 
TTTAGTTTCC CTTCGGAGAA TAAGCCCCAG TACATCCACA TAACAGGTAC CGTCTTCTTG 
CAGCTGCCAT ACTTCAAGAG GAAGCATTCA AGTGGGCAGC AACGTCGACG CCGGAACTCT 
ACCACTTCGA ACAACCAGGG GCTGTTTGGT GGGGAGGAGT GTGTTGGCTA CTATTGGGCC 
TACAACACTA TGCTGACCAA GTCCTGGAGG GCAGGCGTGC TTGGGGATGA GAAGCTCGCC 
GACCGCCTCC TCAGAGACTT CAGCGACTTC TGTGCCAACA AAGACAACAG ACTGTTAAAC 
TTCTGGGACA GCTGTCAGGA GAAAATGAAC ACCAGCACTC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011614624.1
CDS	216..4940
Translation	MRTNKSYKLVLHKKGFGGSDDELVVNPKVFPQVCLRDIIEIAHPTDEYSPLLLQVKNLKEDLQKETISVDQTVAQAFKLRAYQDVIVNVINPKDVTLDLVELTFKDQYIGRGDMWRLKKCLVSTCAYVTQKVEFAGIRAQASELWLKGEKVTCGYISEDTRVVFRSTSAMVYIFIQMSSEMWDFDIYGDLYFEKAVNGFLSDLFAKWKEKNCSHEVTVVLFSRTFYNAKTVDEFPEVLRGSIRQDQECRFFEDFYRVVAQNERRDEWMSLLVSIKKLFVKYPLLVRLEETDGGPVGYNSTAAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRQLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRMMGVDSHVGDDYHLPHWINHSFYTSKSQTSCSSFTPRITVAGLKLTTEKSRFNKDHTLCSPKDTESSLPIQVDYDAHDIQVFRLPGPSRFKRTPNFRLGRDKEIGGRKSLGSLEVTACIGVSPPVRFEGPEEHKSVTSNDSLGSVSNVLLIPRLPSAQYEVSSSLGYTSTREMLEKMESQRDSSAPGRFTVGSAESPRHIRPGCYTPQRALINPFTPSRMPMKLTSNRRRWMHTFPIGPSGEAIQIHHQMRQNMADLQGQQTNPAHSSAELLELVCPEATGRKTLSGQVGETGLYVGGLMEELTANPESNGGLPTHCASLFDDCPLSSADPMPSFCCTVGVDWKSLTTPACLPLTTDYFPDRQTLQNDYTEGCYDLLPHSDLDRREEEATVRTASEVFEEFICQRLMQGYQIIVQTHNRKPQPSVSTPLGSSPLYSRGLVSLRRAEEKENVYWLSMGRTFHKVCLKDKIISVTRYLPKYPCETAQIQYSYSLCPSHSEGQFVSCWVEFGHERLEEYKWNYLDQYICSAGSEDFSLIDSLKFWRTRFLLLPAGGARRMADGEGHWDIYGEAVGTAMGGSGDWVLLDNFIRFLEGLNRIRRRHRSDRIIRQKGTPMKGLQVAGTLPSYSSEHMAPPQGKKGTSALSVLLEMEQKTLDEQQGTKPSTAVADPSGIATNLMYLDSPRKDGGFILEFIRSPHSSNTYHSQLPVEASEAADRGLQPTVAPGAAVQPVGELASCSNTGDTSGNSAVQSLSLSSSPTLMEILEAIKHPMTGVQLLPEQRGLPLNCFFSAEVVHWLINNVEGVTTQGIAVDIMQKMLDEGLVAHASGDMMRTFIYGFYCYRIVGEKDGSNSQPPSTTAGGWPAAALEDFALFQGKWFEVAFVVEEQLHCDLPVFLLPWLPSRPSSYASRHSSFSRSFGGRSQAAALLAATVPEQKMVTLDVDVNNRSDRMEWCSCYYHGNFFLNTAFEIKLHWMAVTAAVLFEMVQGWHRKAASCGFLLIPALEFPFALTSSLYGDPLRAQHFISLNIHCLLKNGSDNLFEGFEPETYWDRMQFFQEAILYRFGFVHDKFSVSTFSFPSENKPQYIHITGTVFLQLPYFKRKHSSGQQRRRRNSTTSNNQGLFGGEECVGYYWAYNTMLTKSWRAGVLGDEKLADRLLRDFSDFCANKDNRLLNFWDSCQEKMNTSTP

Target ORF information:

RefSeq Version	XM_011616322.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011616322.2

ORF Insert Sequence:

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG GTCCTGAAGA GCATAAAAGT 
GTAACATCAA ATGATAGTTT AGGCTCCGTG TCCAATGTGC TGCTCATTCC CCGTCTACCT 
TCAGCCCAGT ATGAAGTTAG CAGTTCCTTG GGATACACGA GCACCAGAGA AATGTTAGAG 
AAGATGGAGT CACAGAGGGA CTCCAGTGCA CCAGGAAGAT TTACAGTGGG GAGTGCTGAG 
TCCCCAAGGC ACATCCGTCC TGGATGCTAC ACCCCCCAAA GAGCACTCAT CAATCCTTTC 
ACCCCATCAA GGATGCCTAT GAAGCTAACC TCCAACCGCC GGCGTTGGAT GCACACCTTC 
CCCATAGGTC CCTCTGGAGA GGCAATTCAA ATCCATCATC AGATGAGACA GAACATGGCT 
GACCTGCAAG GCCAACAGAC AAATCCTGCA CATAGCTCAG CTGAGCTGTT GGAACTCGTC 
TGTCCTGAGG CCACTGGAAG AAAAACACTC TCAGGACAAG TGGGAGAAAC TGGCCTTTAT 
GTTGGGGGAT TGATGGAAGA ATTGACTGCA AATCCAGAGA GTAATGGTGG ACTTCCAACC 
CACTGTGCCT CCTTATTTGA TGACTGTCCC CTCAGCAGTG CTGACCCAAT GCCCAGCTTT 
TGTTGTACAG TGGGGGTGGA TTGGAAGTCT TTGACGACAC CAGCTTGTCT GCCCCTCACC 
ACGGACTATT TTCCAGATCG CCAAACCCTT CAGAATGATT ATACTGAAGG CTGCTATGAC 
CTGCTACCAC ACAGTGACCT GGACAGGCGG GAAGAAGAAG CAACTGTAAG AACAGCTTCC 
GAAGTGTTTG AGGAGTTTAT CTGTCAGAGA CTGATGCAGG GGTATCAAAT CATTGTTCAG 
ACTCATAACA GGAAGCCTCA GCCATCTGTG TCCACACCTC TTGGCAGCAG CCCTCTTTAC 
TCCAGAGGCT TGGTTTCCCT GCGTCGAGCA GAAGAGAAGG AGAATGTTTA TTGGCTCAGC 
ATGGGCCGTA CCTTCCATAA AGTTTGCCTC AAAGACAAAA TAATCAGTGT AACTCGCTAT 
TTGCCAAAGT ACCCGTGTGA GACTGCTCAG ATCCAGTACA GTTACAGCCT TTGTCCATCA 
CACTCAGAGG GTCAATTTGT GTCCTGTTGG GTGGAGTTTG GTCACGAACG GCTGGAAGAA 
TACAAGTGGA ACTATCTGGA CCAGTACATC TGCTCTGCTG GGTCTGAGGA CTTTAGTTTG 
ATAGACTCTC TAAAGTTTTG GAGGACTCGG TTCTTGCTTC TGCCTGCTGG GGGAGCAAGG 
CGAATGGCAG ATGGTGAAGG GCACTGGGAT ATTTATGGAG AGGCAGTGGG TACTGCAATG 
GGCGGATCTG GGGACTGGGT CTTGCTGGAC AACTTCATCC GCTTCCTGGA GGGCCTGAAC 
CGCATTCGTC GCCGACATCG CTCGGACAGG ATCATTAGAC AAAAGGGGAC ACCAATGAAA 
GGCCTGCAGG TTGCTGGTAC TCTCCCTTCT TATTCTTCTG AACACATGGC TCCTCCTCAA 
GGCAAAAAGG GCACATCTGC ATTATCAGTC TTACTGGAGA TGGAGCAGAA GACACTGGAT 
GAACAGCAGG GAACAAAGCC CTCAACAGCT GTTGCTGACC CCTCTGGTAT TGCAACAAAT 
CTCATGTATC TGGACAGTCC TCGCAAGGAT GGTGGCTTCA TTTTGGAATT TATTCGTAGC 
CCTCATTCTT CCAACACCTA TCACTCTCAG TTGCCTGTTG AAGCCAGTGA AGCGGCGGAC 
AGAGGACTCC AGCCAACAGT TGCACCTGGA GCAGCAGTCC AGCCTGTAGG AGAGCTTGCA 
TCTTGTAGCA ACACAGGAGA CACCAGTGGA AATTCTGCAG TGCAGTCGCT TTCTTTGTCA 
TCATCCCCAA CACTCATGGA GATCCTGGAG GCTATAAAAC ATCCAATGAC AGGTGTACAA 
CTCCTTCCAG AGCAAAGAGG CCTGCCTCTC AATTGCTTTT TTAGTGCGGA GGTGGTTCAC 
TGGCTGATTA ACAACGTGGA AGGGGTAACG ACACAAGGCA TAGCTGTAGA TATCATGCAG 
AAAATGTTGG ATGAAGGTTT GGTGGCCCAT GCTTCTGGAG ACATGATGCG TACCTTCATT 
TATGGATTCT ACTGCTACAG GATTGTTGGA GAGAAGGATG GTTCAAATTC CCAGCCTCCT 
TCCACAACAG CAGGGGGCTG GCCTGCAGCA GCTCTGGAGG ACTTTGCCCT GTTCCAGGGA 
AAGTGGTTTG AGGTGGCCTT TGTGGTGGAA GAGCAGCTCC ATTGCGATCT CCCAGTTTTT 
CTCCTGCCCT GGCTCCCCAG CAGGCCGTCC TCCTATGCAA GTAGGCATAG CTCATTCAGC 
CGCAGTTTTG GAGGACGCAG TCAGGCCGCT GCATTGCTAG CTGCCACTGT TCCAGAGCAG 
AAAATGGTCA CTCTGGATGT GGATGTGAAC AATCGCAGTG ACCGGATGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT CTTCCTCAAC ACTGCCTTTG AGATCAAGCT TCACTGGATG 
GCTGTCACTG CTGCAGTACT CTTTGAGATG GTTCAGGGCT GGCACAGGAA AGCAGCATCA 
TGTGGCTTCC TCTTGATCCC TGCACTGGAG TTTCCTTTTG CACTAACCTC CTCCCTGTAT 
GGAGATCCCC TCAGAGCCCA GCACTTCATT TCTCTGAACA TCCACTGCCT TCTAAAGAAT 
GGCAGTGACA ATCTGTTTGA AGGCTTTGAA CCAGAGACCT ACTGGGATAG GATGCAGTTC 
TTTCAGGAGG CCATACTTTA CAGGTTTGGA TTTGTGCATG ACAAATTTTC AGTTTCAACC 
TTTAGTTTCC CTTCGGAGAA TAAGCCCCAG TACATCCACA TAACAGGTAC CGTCTTCTTG 
CAGCTGCCAT ACTTCAAGAG GAAGCATTCA AGTGGGCAGC AACGTCGACG CCGGAACTCT 
ACCACTTCGA ACAACCAGGG GCTGTTTGGT GGGGAGGAGT GTGTTGGCTA CTATTGGGCC 
TACAACACTA TGCTGACCAA GTCCTGGAGG GCAGGCGTGC TTGGGGATGA GAAGCTCGCC 
GACCGCCTCC TCAGAGACTT CAGCGACTTC TGTGCCAACA AAGACAACAG ACTGTTAAAC 
TTCTGGGACA GCTGTCAGGA GAAAATGAAC ACCAGCACTC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

depdc5 ( XM_011616323.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011614625.1 Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X2, mRNA.

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CloneID	OTo21240
Clone ID Related Accession (Same CDS sequence)	XM_011616322.1 , XM_011616322.2 , XM_011616323.1
Accession Version	XM_011616323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	DEP domain-containing protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071163.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG GTCCTGAAGA GCATAAAAGT 
GTAACATCAA ATGATAGTTT AGGCTCCGTG TCCAATGTGC TGCTCATTCC CCGTCTACCT 
TCAGCCCAGT ATGAAGTTAG CAGTTCCTTG GGATACACGA GCACCAGAGA AATGTTAGAG 
AAGATGGAGT CACAGAGGGA CTCCAGTGCA CCAGGAAGAT TTACAGTGGG GAGTGCTGAG 
TCCCCAAGGC ACATCCGTCC TGGATGCTAC ACCCCCCAAA GAGCACTCAT CAATCCTTTC 
ACCCCATCAA GGATGCCTAT GAAGCTAACC TCCAACCGCC GGCGTTGGAT GCACACCTTC 
CCCATAGGTC CCTCTGGAGA GGCAATTCAA ATCCATCATC AGATGAGACA GAACATGGCT 
GACCTGCAAG GCCAACAGAC AAATCCTGCA CATAGCTCAG CTGAGCTGTT GGAACTCGTC 
TGTCCTGAGG CCACTGGAAG AAAAACACTC TCAGGACAAG TGGGAGAAAC TGGCCTTTAT 
GTTGGGGGAT TGATGGAAGA ATTGACTGCA AATCCAGAGA GTAATGGTGG ACTTCCAACC 
CACTGTGCCT CCTTATTTGA TGACTGTCCC CTCAGCAGTG CTGACCCAAT GCCCAGCTTT 
TGTTGTACAG TGGGGGTGGA TTGGAAGTCT TTGACGACAC CAGCTTGTCT GCCCCTCACC 
ACGGACTATT TTCCAGATCG CCAAACCCTT CAGAATGATT ATACTGAAGG CTGCTATGAC 
CTGCTACCAC ACAGTGACCT GGACAGGCGG GAAGAAGAAG CAACTGTAAG AACAGCTTCC 
GAAGTGTTTG AGGAGTTTAT CTGTCAGAGA CTGATGCAGG GGTATCAAAT CATTGTTCAG 
ACTCATAACA GGAAGCCTCA GCCATCTGTG TCCACACCTC TTGGCAGCAG CCCTCTTTAC 
TCCAGAGGCT TGGTTTCCCT GCGTCGAGCA GAAGAGAAGG AGAATGTTTA TTGGCTCAGC 
ATGGGCCGTA CCTTCCATAA AGTTTGCCTC AAAGACAAAA TAATCAGTGT AACTCGCTAT 
TTGCCAAAGT ACCCGTGTGA GACTGCTCAG ATCCAGTACA GTTACAGCCT TTGTCCATCA 
CACTCAGAGG GTCAATTTGT GTCCTGTTGG GTGGAGTTTG GTCACGAACG GCTGGAAGAA 
TACAAGTGGA ACTATCTGGA CCAGTACATC TGCTCTGCTG GGTCTGAGGA CTTTAGTTTG 
ATAGACTCTC TAAAGTTTTG GAGGACTCGG TTCTTGCTTC TGCCTGCTGG GGGAGCAAGG 
CGAATGGCAG ATGGTGAAGG GCACTGGGAT ATTTATGGAG AGGCAGTGGG TACTGCAATG 
GGCGGATCTG GGGACTGGGT CTTGCTGGAC AACTTCATCC GCTTCCTGGA GGGCCTGAAC 
CGCATTCGTC GCCGACATCG CTCGGACAGG ATCATTAGAC AAAAGGGGAC ACCAATGAAA 
GGCCTGCAGG TTGCTGGTAC TCTCCCTTCT TATTCTTCTG AACACATGGC TCCTCCTCAA 
GGCAAAAAGG GCACATCTGC ATTATCAGTC TTACTGGAGA TGGAGCAGAA GACACTGGAT 
GAACAGCAGG GAACAAAGCC CTCAACAGCT GTTGCTGACC CCTCTGGTAT TGCAACAAAT 
CTCATGTATC TGGACAGTCC TCGCAAGGAT GGTGGCTTCA TTTTGGAATT TATTCGTAGC 
CCTCATTCTT CCAACACCTA TCACTCTCAG TTGCCTGTTG AAGCCAGTGA AGCGGCGGAC 
AGAGGACTCC AGCCAACAGT TGCACCTGGA GCAGCAGTCC AGCCTGTAGG AGAGCTTGCA 
TCTTGTAGCA ACACAGGAGA CACCAGTGGA AATTCTGCAG TGCAGTCGCT TTCTTTGTCA 
TCATCCCCAA CACTCATGGA GATCCTGGAG GCTATAAAAC ATCCAATGAC AGGTGTACAA 
CTCCTTCCAG AGCAAAGAGG CCTGCCTCTC AATTGCTTTT TTAGTGCGGA GGTGGTTCAC 
TGGCTGATTA ACAACGTGGA AGGGGTAACG ACACAAGGCA TAGCTGTAGA TATCATGCAG 
AAAATGTTGG ATGAAGGTTT GGTGGCCCAT GCTTCTGGAG ACATGATGCG TACCTTCATT 
TATGGATTCT ACTGCTACAG GATTGTTGGA GAGAAGGATG GTTCAAATTC CCAGCCTCCT 
TCCACAACAG CAGGGGGCTG GCCTGCAGCA GCTCTGGAGG ACTTTGCCCT GTTCCAGGGA 
AAGTGGTTTG AGGTGGCCTT TGTGGTGGAA GAGCAGCTCC ATTGCGATCT CCCAGTTTTT 
CTCCTGCCCT GGCTCCCCAG CAGGCCGTCC TCCTATGCAA GTAGGCATAG CTCATTCAGC 
CGCAGTTTTG GAGGACGCAG TCAGGCCGCT GCATTGCTAG CTGCCACTGT TCCAGAGCAG 
AAAATGGTCA CTCTGGATGT GGATGTGAAC AATCGCAGTG ACCGGATGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT CTTCCTCAAC ACTGCCTTTG AGATCAAGCT TCACTGGATG 
GCTGTCACTG CTGCAGTACT CTTTGAGATG GTTCAGGGCT GGCACAGGAA AGCAGCATCA 
TGTGGCTTCC TCTTGATCCC TGCACTGGAG TTTCCTTTTG CACTAACCTC CTCCCTGTAT 
GGAGATCCCC TCAGAGCCCA GCACTTCATT TCTCTGAACA TCCACTGCCT TCTAAAGAAT 
GGCAGTGACA ATCTGTTTGA AGGCTTTGAA CCAGAGACCT ACTGGGATAG GATGCAGTTC 
TTTCAGGAGG CCATACTTTA CAGGTTTGGA TTTGTGCATG ACAAATTTTC AGTTTCAACC 
TTTAGTTTCC CTTCGGAGAA TAAGCCCCAG TACATCCACA TAACAGGTAC CGTCTTCTTG 
CAGCTGCCAT ACTTCAAGAG GAAGCATTCA AGTGGGCAGC AACGTCGACG CCGGAACTCT 
ACCACTTCGA ACAACCAGGG GCTGTTTGGT GGGGAGGAGT GTGTTGGCTA CTATTGGGCC 
TACAACACTA TGCTGACCAA GTCCTGGAGG GCAGGCGTGC TTGGGGATGA GAAGCTCGCC 
GACCGCCTCC TCAGAGACTT CAGCGACTTC TGTGCCAACA AAGACAACAG ACTGTTAAAC 
TTCTGGGACA GCTGTCAGGA GAAAATGAAC ACCAGCACTC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011614625.1
CDS	41..4765
Translation	MRTNKSYKLVLHKKGFGGSDDELVVNPKVFPQVCLRDIIEIAHPTDEYSPLLLQVKNLKEDLQKETISVDQTVAQAFKLRAYQDVIVNVINPKDVTLDLVELTFKDQYIGRGDMWRLKKCLVSTCAYVTQKVEFAGIRAQASELWLKGEKVTCGYISEDTRVVFRSTSAMVYIFIQMSSEMWDFDIYGDLYFEKAVNGFLSDLFAKWKEKNCSHEVTVVLFSRTFYNAKTVDEFPEVLRGSIRQDQECRFFEDFYRVVAQNERRDEWMSLLVSIKKLFVKYPLLVRLEETDGGPVGYNSTAAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRQLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRMMGVDSHVGDDYHLPHWINHSFYTSKSQTSCSSFTPRITVAGLKLTTEKSRFNKDHTLCSPKDTESSLPIQVDYDAHDIQVFRLPGPSRFKRTPNFRLGRDKEIGGRKSLGSLEVTACIGVSPPVRFEGPEEHKSVTSNDSLGSVSNVLLIPRLPSAQYEVSSSLGYTSTREMLEKMESQRDSSAPGRFTVGSAESPRHIRPGCYTPQRALINPFTPSRMPMKLTSNRRRWMHTFPIGPSGEAIQIHHQMRQNMADLQGQQTNPAHSSAELLELVCPEATGRKTLSGQVGETGLYVGGLMEELTANPESNGGLPTHCASLFDDCPLSSADPMPSFCCTVGVDWKSLTTPACLPLTTDYFPDRQTLQNDYTEGCYDLLPHSDLDRREEEATVRTASEVFEEFICQRLMQGYQIIVQTHNRKPQPSVSTPLGSSPLYSRGLVSLRRAEEKENVYWLSMGRTFHKVCLKDKIISVTRYLPKYPCETAQIQYSYSLCPSHSEGQFVSCWVEFGHERLEEYKWNYLDQYICSAGSEDFSLIDSLKFWRTRFLLLPAGGARRMADGEGHWDIYGEAVGTAMGGSGDWVLLDNFIRFLEGLNRIRRRHRSDRIIRQKGTPMKGLQVAGTLPSYSSEHMAPPQGKKGTSALSVLLEMEQKTLDEQQGTKPSTAVADPSGIATNLMYLDSPRKDGGFILEFIRSPHSSNTYHSQLPVEASEAADRGLQPTVAPGAAVQPVGELASCSNTGDTSGNSAVQSLSLSSSPTLMEILEAIKHPMTGVQLLPEQRGLPLNCFFSAEVVHWLINNVEGVTTQGIAVDIMQKMLDEGLVAHASGDMMRTFIYGFYCYRIVGEKDGSNSQPPSTTAGGWPAAALEDFALFQGKWFEVAFVVEEQLHCDLPVFLLPWLPSRPSSYASRHSSFSRSFGGRSQAAALLAATVPEQKMVTLDVDVNNRSDRMEWCSCYYHGNFFLNTAFEIKLHWMAVTAAVLFEMVQGWHRKAASCGFLLIPALEFPFALTSSLYGDPLRAQHFISLNIHCLLKNGSDNLFEGFEPETYWDRMQFFQEAILYRFGFVHDKFSVSTFSFPSENKPQYIHITGTVFLQLPYFKRKHSSGQQRRRRNSTTSNNQGLFGGEECVGYYWAYNTMLTKSWRAGVLGDEKLADRLLRDFSDFCANKDNRLLNFWDSCQEKMNTSTP

Target ORF information:

RefSeq Version	XM_011616323.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011616323.1

ORF Insert Sequence:

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG GTCCTGAAGA GCATAAAAGT 
GTAACATCAA ATGATAGTTT AGGCTCCGTG TCCAATGTGC TGCTCATTCC CCGTCTACCT 
TCAGCCCAGT ATGAAGTTAG CAGTTCCTTG GGATACACGA GCACCAGAGA AATGTTAGAG 
AAGATGGAGT CACAGAGGGA CTCCAGTGCA CCAGGAAGAT TTACAGTGGG GAGTGCTGAG 
TCCCCAAGGC ACATCCGTCC TGGATGCTAC ACCCCCCAAA GAGCACTCAT CAATCCTTTC 
ACCCCATCAA GGATGCCTAT GAAGCTAACC TCCAACCGCC GGCGTTGGAT GCACACCTTC 
CCCATAGGTC CCTCTGGAGA GGCAATTCAA ATCCATCATC AGATGAGACA GAACATGGCT 
GACCTGCAAG GCCAACAGAC AAATCCTGCA CATAGCTCAG CTGAGCTGTT GGAACTCGTC 
TGTCCTGAGG CCACTGGAAG AAAAACACTC TCAGGACAAG TGGGAGAAAC TGGCCTTTAT 
GTTGGGGGAT TGATGGAAGA ATTGACTGCA AATCCAGAGA GTAATGGTGG ACTTCCAACC 
CACTGTGCCT CCTTATTTGA TGACTGTCCC CTCAGCAGTG CTGACCCAAT GCCCAGCTTT 
TGTTGTACAG TGGGGGTGGA TTGGAAGTCT TTGACGACAC CAGCTTGTCT GCCCCTCACC 
ACGGACTATT TTCCAGATCG CCAAACCCTT CAGAATGATT ATACTGAAGG CTGCTATGAC 
CTGCTACCAC ACAGTGACCT GGACAGGCGG GAAGAAGAAG CAACTGTAAG AACAGCTTCC 
GAAGTGTTTG AGGAGTTTAT CTGTCAGAGA CTGATGCAGG GGTATCAAAT CATTGTTCAG 
ACTCATAACA GGAAGCCTCA GCCATCTGTG TCCACACCTC TTGGCAGCAG CCCTCTTTAC 
TCCAGAGGCT TGGTTTCCCT GCGTCGAGCA GAAGAGAAGG AGAATGTTTA TTGGCTCAGC 
ATGGGCCGTA CCTTCCATAA AGTTTGCCTC AAAGACAAAA TAATCAGTGT AACTCGCTAT 
TTGCCAAAGT ACCCGTGTGA GACTGCTCAG ATCCAGTACA GTTACAGCCT TTGTCCATCA 
CACTCAGAGG GTCAATTTGT GTCCTGTTGG GTGGAGTTTG GTCACGAACG GCTGGAAGAA 
TACAAGTGGA ACTATCTGGA CCAGTACATC TGCTCTGCTG GGTCTGAGGA CTTTAGTTTG 
ATAGACTCTC TAAAGTTTTG GAGGACTCGG TTCTTGCTTC TGCCTGCTGG GGGAGCAAGG 
CGAATGGCAG ATGGTGAAGG GCACTGGGAT ATTTATGGAG AGGCAGTGGG TACTGCAATG 
GGCGGATCTG GGGACTGGGT CTTGCTGGAC AACTTCATCC GCTTCCTGGA GGGCCTGAAC 
CGCATTCGTC GCCGACATCG CTCGGACAGG ATCATTAGAC AAAAGGGGAC ACCAATGAAA 
GGCCTGCAGG TTGCTGGTAC TCTCCCTTCT TATTCTTCTG AACACATGGC TCCTCCTCAA 
GGCAAAAAGG GCACATCTGC ATTATCAGTC TTACTGGAGA TGGAGCAGAA GACACTGGAT 
GAACAGCAGG GAACAAAGCC CTCAACAGCT GTTGCTGACC CCTCTGGTAT TGCAACAAAT 
CTCATGTATC TGGACAGTCC TCGCAAGGAT GGTGGCTTCA TTTTGGAATT TATTCGTAGC 
CCTCATTCTT CCAACACCTA TCACTCTCAG TTGCCTGTTG AAGCCAGTGA AGCGGCGGAC 
AGAGGACTCC AGCCAACAGT TGCACCTGGA GCAGCAGTCC AGCCTGTAGG AGAGCTTGCA 
TCTTGTAGCA ACACAGGAGA CACCAGTGGA AATTCTGCAG TGCAGTCGCT TTCTTTGTCA 
TCATCCCCAA CACTCATGGA GATCCTGGAG GCTATAAAAC ATCCAATGAC AGGTGTACAA 
CTCCTTCCAG AGCAAAGAGG CCTGCCTCTC AATTGCTTTT TTAGTGCGGA GGTGGTTCAC 
TGGCTGATTA ACAACGTGGA AGGGGTAACG ACACAAGGCA TAGCTGTAGA TATCATGCAG 
AAAATGTTGG ATGAAGGTTT GGTGGCCCAT GCTTCTGGAG ACATGATGCG TACCTTCATT 
TATGGATTCT ACTGCTACAG GATTGTTGGA GAGAAGGATG GTTCAAATTC CCAGCCTCCT 
TCCACAACAG CAGGGGGCTG GCCTGCAGCA GCTCTGGAGG ACTTTGCCCT GTTCCAGGGA 
AAGTGGTTTG AGGTGGCCTT TGTGGTGGAA GAGCAGCTCC ATTGCGATCT CCCAGTTTTT 
CTCCTGCCCT GGCTCCCCAG CAGGCCGTCC TCCTATGCAA GTAGGCATAG CTCATTCAGC 
CGCAGTTTTG GAGGACGCAG TCAGGCCGCT GCATTGCTAG CTGCCACTGT TCCAGAGCAG 
AAAATGGTCA CTCTGGATGT GGATGTGAAC AATCGCAGTG ACCGGATGGA GTGGTGCAGC 
TGTTATTACC ATGGCAACTT CTTCCTCAAC ACTGCCTTTG AGATCAAGCT TCACTGGATG 
GCTGTCACTG CTGCAGTACT CTTTGAGATG GTTCAGGGCT GGCACAGGAA AGCAGCATCA 
TGTGGCTTCC TCTTGATCCC TGCACTGGAG TTTCCTTTTG CACTAACCTC CTCCCTGTAT 
GGAGATCCCC TCAGAGCCCA GCACTTCATT TCTCTGAACA TCCACTGCCT TCTAAAGAAT 
GGCAGTGACA ATCTGTTTGA AGGCTTTGAA CCAGAGACCT ACTGGGATAG GATGCAGTTC 
TTTCAGGAGG CCATACTTTA CAGGTTTGGA TTTGTGCATG ACAAATTTTC AGTTTCAACC 
TTTAGTTTCC CTTCGGAGAA TAAGCCCCAG TACATCCACA TAACAGGTAC CGTCTTCTTG 
CAGCTGCCAT ACTTCAAGAG GAAGCATTCA AGTGGGCAGC AACGTCGACG CCGGAACTCT 
ACCACTTCGA ACAACCAGGG GCTGTTTGGT GGGGAGGAGT GTGTTGGCTA CTATTGGGCC 
TACAACACTA TGCTGACCAA GTCCTGGAGG GCAGGCGTGC TTGGGGATGA GAAGCTCGCC 
GACCGCCTCC TCAGAGACTT CAGCGACTTC TGTGCCAACA AAGACAACAG ACTGTTAAAC 
TTCTGGGACA GCTGTCAGGA GAAAATGAAC ACCAGCACTC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

depdc5 ( XM_011616324.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011614626.1 Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X3, mRNA.

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CloneID	OTo21241
Clone ID Related Accession (Same CDS sequence)	XM_011616324.1
Accession Version	XM_011616324.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	DEP domain-containing protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071163.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG GTCCTGAAGA GCATAAAAGT 
GTAACATCAA ATGATAGTTT AGGCTCCGTG TCCAATGTGC TGCTCATTCC CCGTCTACCT 
TCAGCCCAGT ATGAAGTTAG CAGTTCCTTG GGATACACGA GCACCAGAGA AATGTTAGAG 
AAGATGGAGT CACAGAGGGA CTCCAGTGCA CCAGGAAGAT TTACAGTGGG GAGTGCTGAG 
TCCCCAAGGC ACATCCGTCC TGGATGCTAC ACCCCCCAAA GAGCACTCAT CAATCCTTTC 
ACCCCATCAA GGATGCCTAT GAAGCTAACC TCCAACCGCC GGCGTTGGAT GCACACCTTC 
CCCATAGGTC CCTCTGGAGA GGCAATTCAA ATCCATCATC AGATGAGACA GAACATGGCT 
GACCTGCAAG GCCAACAGAC AAATCCTGCA CATAGCTCAG CTGAGCTGTT GGAACTCGTC 
TGTCCTGAGG CCACTGGAAG AAAAACACTC TCAGGACAAG TGGGAGAAAC TGGCCTTTAT 
GTTGGGGGAT TGATGGAAGA ATTGACTGCA AATCCAGAGA GTAATGGTGG ACTTCCAACC 
CACTGTGCCT CCTTATTTGA TGACTGTCCC CTCAGCAGTG CTGACCCAAT GCCCAGCTTT 
TGTTGTACAG TGGGGGTGGA TTGGAAGTCT TTGACGACAC CAGCTTGTCT GCCCCTCACC 
ACGGACTATT TTCCAGATCG CCAAACCCTT CAGAATGATT ATACTGAAGG CTGCTATGAC 
CTGCTACCAC ACAGTGACCT GGACAGGCGG GAAGAAGAAG CAACTGTAAG AACAGCTTCC 
GAAGTGTTTG AGGAGTTTAT CTGTCAGAGA CTGATGCAGG GGTATCAAAT CATTGTTCAG 
ACTCATAACA GGAAGCCTCA GCCATCTGTG TCCACACCTC TTGGCAGCAG CCCTCTTTAC 
TCCAGAGGCT TGGTTTCCCT GCGTCGAGCA GAAGAGAAGG AGAATGTTTA TTGGCTCAGC 
ATGGGCCGTA CCTTCCATAA AGTTTGCCTC AAAGACAAAA TAATCAGTGT AACTCGCTAT 
TTGCCAAAGT ACCCGTGTGA GACTGCTCAG ATCCAGTACA GTTACAGCCT TTGTCCATCA 
CACTCAGAGG GTCAATTTGT GTCCTGTTGG GTGGAGTTTG GTCACGAACG GCTGGAAGAA 
TACAAGTGGA ACTATCTGGA CCAGTACATC TGCTCTGCTG GGTCTGAGGA CTTTAGTTTG 
ATAGACTCTC TAAAGTTTTG GAGGACTCGG TTCTTGCTTC TGCCTGCTGG GGGAGCAAGG 
CGAATGGCAG ATGGTGAAGG GCACTGGGAT ATTTATGGAG AGGCAGTGGG TACTGCAATG 
GGCGGATCTG GGGACTGGGT CTTGCTGGAC AACTTCATCC GCTTCCTGGA GGGCCTGAAC 
CGCATTCGTC GCCGACATCG CTCGGACAGG ATCATTAGAC AAAAGGGGAC ACCAATGAAA 
GGCCTGCAGG TTGCTGGTAC TCTCCCTTCT TATTCTTCTG AACACATGGC TCCTCCTCAA 
GGCAAAAAGG GCACATCTGC ATTATCAGTC TTACTGGAGA TGGAGCAGAA GACACTGGAT 
GAACAGCAGG GAACAAAGCC CTCAACAGCT GTTGCTGACC CCTCTGGTAT TGCAACAAAT 
CTCATGTATC TGGACAGTCC TCGCAAGTTG CCTGTTGAAG CCAGTGAAGC GGCGGACAGA 
GGACTCCAGC CAACAGTTGC ACCTGGAGCA GCAGTCCAGC CTGTAGGAGA GCTTGCATCT 
TGTAGCAACA CAGGAGACAC CAGTGGAAAT TCTGCAGTGC AGTCGCTTTC TTTGTCATCA 
TCCCCAACAC TCATGGAGAT CCTGGAGGCT ATAAAACATC CAATGACAGG TGTACAACTC 
CTTCCAGAGC AAAGAGGCCT GCCTCTCAAT TGCTTTTTTA GTGCGGAGGT GGTTCACTGG 
CTGATTAACA ACGTGGAAGG GGTAACGACA CAAGGCATAG CTGTAGATAT CATGCAGAAA 
ATGTTGGATG AAGGTTTGGT GGCCCATGCT TCTGGAGACA TGATGCGTAC CTTCATTTAT 
GGATTCTACT GCTACAGGAT TGTTGGAGAG AAGGATGGTT CAAATTCCCA GCCTCCTTCC 
ACAACAGCAG GGGGCTGGCC TGCAGCAGCT CTGGAGGACT TTGCCCTGTT CCAGGGAAAG 
TGGTTTGAGG TGGCCTTTGT GGTGGAAGAG CAGCTCCATT GCGATCTCCC AGTTTTTCTC 
CTGCCCTGGC TCCCCAGCAG GCCGTCCTCC TATGCAAGTA GGCATAGCTC ATTCAGCCGC 
AGTTTTGGAG GACGCAGTCA GGCCGCTGCA TTGCTAGCTG CCACTGTTCC AGAGCAGAAA 
ATGGTCACTC TGGATGTGGA TGTGAACAAT CGCAGTGACC GGATGGAGTG GTGCAGCTGT 
TATTACCATG GCAACTTCTT CCTCAACACT GCCTTTGAGA TCAAGCTTCA CTGGATGGCT 
GTCACTGCTG CAGTACTCTT TGAGATGGTT CAGGGCTGGC ACAGGAAAGC AGCATCATGT 
GGCTTCCTCT TGATCCCTGC ACTGGAGTTT CCTTTTGCAC TAACCTCCTC CCTGTATGGA 
GATCCCCTCA GAGCCCAGCA CTTCATTTCT CTGAACATCC ACTGCCTTCT AAAGAATGGC 
AGTGACAATC TGTTTGAAGG CTTTGAACCA GAGACCTACT GGGATAGGAT GCAGTTCTTT 
CAGGAGGCCA TACTTTACAG GTTTGGATTT GTGCATGACA AATTTTCAGT TTCAACCTTT 
AGTTTCCCTT CGGAGAATAA GCCCCAGTAC ATCCACATAA CAGGTACCGT CTTCTTGCAG 
CTGCCATACT TCAAGAGGAA GCATTCAAGT GGGCAGCAAC GTCGACGCCG GAACTCTACC 
ACTTCGAACA ACCAGGGGCT GTTTGGTGGG GAGGAGTGTG TTGGCTACTA TTGGGCCTAC 
AACACTATGC TGACCAAGTC CTGGAGGGCA GGCGTGCTTG GGGATGAGAA GCTCGCCGAC 
CGCCTCCTCA GAGACTTCAG CGACTTCTGT GCCAACAAAG ACAACAGACT GTTAAACTTC 
TGGGACAGCT GTCAGGAGAA AATGAACACC AGCACTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011614626.1
CDS	207..4868
Translation	MRTNKSYKLVLHKKGFGGSDDELVVNPKVFPQVCLRDIIEIAHPTDEYSPLLLQVKNLKEDLQKETISVDQTVAQAFKLRAYQDVIVNVINPKDVTLDLVELTFKDQYIGRGDMWRLKKCLVSTCAYVTQKVEFAGIRAQASELWLKGEKVTCGYISEDTRVVFRSTSAMVYIFIQMSSEMWDFDIYGDLYFEKAVNGFLSDLFAKWKEKNCSHEVTVVLFSRTFYNAKTVDEFPEVLRGSIRQDQECRFFEDFYRVVAQNERRDEWMSLLVSIKKLFVKYPLLVRLEETDGGPVGYNSTAAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRQLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRMMGVDSHVGDDYHLPHWINHSFYTSKSQTSCSSFTPRITVAGLKLTTEKSRFNKDHTLCSPKDTESSLPIQVDYDAHDIQVFRLPGPSRFKRTPNFRLGRDKEIGGRKSLGSLEVTACIGVSPPVRFEGPEEHKSVTSNDSLGSVSNVLLIPRLPSAQYEVSSSLGYTSTREMLEKMESQRDSSAPGRFTVGSAESPRHIRPGCYTPQRALINPFTPSRMPMKLTSNRRRWMHTFPIGPSGEAIQIHHQMRQNMADLQGQQTNPAHSSAELLELVCPEATGRKTLSGQVGETGLYVGGLMEELTANPESNGGLPTHCASLFDDCPLSSADPMPSFCCTVGVDWKSLTTPACLPLTTDYFPDRQTLQNDYTEGCYDLLPHSDLDRREEEATVRTASEVFEEFICQRLMQGYQIIVQTHNRKPQPSVSTPLGSSPLYSRGLVSLRRAEEKENVYWLSMGRTFHKVCLKDKIISVTRYLPKYPCETAQIQYSYSLCPSHSEGQFVSCWVEFGHERLEEYKWNYLDQYICSAGSEDFSLIDSLKFWRTRFLLLPAGGARRMADGEGHWDIYGEAVGTAMGGSGDWVLLDNFIRFLEGLNRIRRRHRSDRIIRQKGTPMKGLQVAGTLPSYSSEHMAPPQGKKGTSALSVLLEMEQKTLDEQQGTKPSTAVADPSGIATNLMYLDSPRKLPVEASEAADRGLQPTVAPGAAVQPVGELASCSNTGDTSGNSAVQSLSLSSSPTLMEILEAIKHPMTGVQLLPEQRGLPLNCFFSAEVVHWLINNVEGVTTQGIAVDIMQKMLDEGLVAHASGDMMRTFIYGFYCYRIVGEKDGSNSQPPSTTAGGWPAAALEDFALFQGKWFEVAFVVEEQLHCDLPVFLLPWLPSRPSSYASRHSSFSRSFGGRSQAAALLAATVPEQKMVTLDVDVNNRSDRMEWCSCYYHGNFFLNTAFEIKLHWMAVTAAVLFEMVQGWHRKAASCGFLLIPALEFPFALTSSLYGDPLRAQHFISLNIHCLLKNGSDNLFEGFEPETYWDRMQFFQEAILYRFGFVHDKFSVSTFSFPSENKPQYIHITGTVFLQLPYFKRKHSSGQQRRRRNSTTSNNQGLFGGEECVGYYWAYNTMLTKSWRAGVLGDEKLADRLLRDFSDFCANKDNRLLNFWDSCQEKMNTSTP

Target ORF information:

RefSeq Version	XM_011616324.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011616324.1

ORF Insert Sequence:

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG GTCCTGAAGA GCATAAAAGT 
GTAACATCAA ATGATAGTTT AGGCTCCGTG TCCAATGTGC TGCTCATTCC CCGTCTACCT 
TCAGCCCAGT ATGAAGTTAG CAGTTCCTTG GGATACACGA GCACCAGAGA AATGTTAGAG 
AAGATGGAGT CACAGAGGGA CTCCAGTGCA CCAGGAAGAT TTACAGTGGG GAGTGCTGAG 
TCCCCAAGGC ACATCCGTCC TGGATGCTAC ACCCCCCAAA GAGCACTCAT CAATCCTTTC 
ACCCCATCAA GGATGCCTAT GAAGCTAACC TCCAACCGCC GGCGTTGGAT GCACACCTTC 
CCCATAGGTC CCTCTGGAGA GGCAATTCAA ATCCATCATC AGATGAGACA GAACATGGCT 
GACCTGCAAG GCCAACAGAC AAATCCTGCA CATAGCTCAG CTGAGCTGTT GGAACTCGTC 
TGTCCTGAGG CCACTGGAAG AAAAACACTC TCAGGACAAG TGGGAGAAAC TGGCCTTTAT 
GTTGGGGGAT TGATGGAAGA ATTGACTGCA AATCCAGAGA GTAATGGTGG ACTTCCAACC 
CACTGTGCCT CCTTATTTGA TGACTGTCCC CTCAGCAGTG CTGACCCAAT GCCCAGCTTT 
TGTTGTACAG TGGGGGTGGA TTGGAAGTCT TTGACGACAC CAGCTTGTCT GCCCCTCACC 
ACGGACTATT TTCCAGATCG CCAAACCCTT CAGAATGATT ATACTGAAGG CTGCTATGAC 
CTGCTACCAC ACAGTGACCT GGACAGGCGG GAAGAAGAAG CAACTGTAAG AACAGCTTCC 
GAAGTGTTTG AGGAGTTTAT CTGTCAGAGA CTGATGCAGG GGTATCAAAT CATTGTTCAG 
ACTCATAACA GGAAGCCTCA GCCATCTGTG TCCACACCTC TTGGCAGCAG CCCTCTTTAC 
TCCAGAGGCT TGGTTTCCCT GCGTCGAGCA GAAGAGAAGG AGAATGTTTA TTGGCTCAGC 
ATGGGCCGTA CCTTCCATAA AGTTTGCCTC AAAGACAAAA TAATCAGTGT AACTCGCTAT 
TTGCCAAAGT ACCCGTGTGA GACTGCTCAG ATCCAGTACA GTTACAGCCT TTGTCCATCA 
CACTCAGAGG GTCAATTTGT GTCCTGTTGG GTGGAGTTTG GTCACGAACG GCTGGAAGAA 
TACAAGTGGA ACTATCTGGA CCAGTACATC TGCTCTGCTG GGTCTGAGGA CTTTAGTTTG 
ATAGACTCTC TAAAGTTTTG GAGGACTCGG TTCTTGCTTC TGCCTGCTGG GGGAGCAAGG 
CGAATGGCAG ATGGTGAAGG GCACTGGGAT ATTTATGGAG AGGCAGTGGG TACTGCAATG 
GGCGGATCTG GGGACTGGGT CTTGCTGGAC AACTTCATCC GCTTCCTGGA GGGCCTGAAC 
CGCATTCGTC GCCGACATCG CTCGGACAGG ATCATTAGAC AAAAGGGGAC ACCAATGAAA 
GGCCTGCAGG TTGCTGGTAC TCTCCCTTCT TATTCTTCTG AACACATGGC TCCTCCTCAA 
GGCAAAAAGG GCACATCTGC ATTATCAGTC TTACTGGAGA TGGAGCAGAA GACACTGGAT 
GAACAGCAGG GAACAAAGCC CTCAACAGCT GTTGCTGACC CCTCTGGTAT TGCAACAAAT 
CTCATGTATC TGGACAGTCC TCGCAAGTTG CCTGTTGAAG CCAGTGAAGC GGCGGACAGA 
GGACTCCAGC CAACAGTTGC ACCTGGAGCA GCAGTCCAGC CTGTAGGAGA GCTTGCATCT 
TGTAGCAACA CAGGAGACAC CAGTGGAAAT TCTGCAGTGC AGTCGCTTTC TTTGTCATCA 
TCCCCAACAC TCATGGAGAT CCTGGAGGCT ATAAAACATC CAATGACAGG TGTACAACTC 
CTTCCAGAGC AAAGAGGCCT GCCTCTCAAT TGCTTTTTTA GTGCGGAGGT GGTTCACTGG 
CTGATTAACA ACGTGGAAGG GGTAACGACA CAAGGCATAG CTGTAGATAT CATGCAGAAA 
ATGTTGGATG AAGGTTTGGT GGCCCATGCT TCTGGAGACA TGATGCGTAC CTTCATTTAT 
GGATTCTACT GCTACAGGAT TGTTGGAGAG AAGGATGGTT CAAATTCCCA GCCTCCTTCC 
ACAACAGCAG GGGGCTGGCC TGCAGCAGCT CTGGAGGACT TTGCCCTGTT CCAGGGAAAG 
TGGTTTGAGG TGGCCTTTGT GGTGGAAGAG CAGCTCCATT GCGATCTCCC AGTTTTTCTC 
CTGCCCTGGC TCCCCAGCAG GCCGTCCTCC TATGCAAGTA GGCATAGCTC ATTCAGCCGC 
AGTTTTGGAG GACGCAGTCA GGCCGCTGCA TTGCTAGCTG CCACTGTTCC AGAGCAGAAA 
ATGGTCACTC TGGATGTGGA TGTGAACAAT CGCAGTGACC GGATGGAGTG GTGCAGCTGT 
TATTACCATG GCAACTTCTT CCTCAACACT GCCTTTGAGA TCAAGCTTCA CTGGATGGCT 
GTCACTGCTG CAGTACTCTT TGAGATGGTT CAGGGCTGGC ACAGGAAAGC AGCATCATGT 
GGCTTCCTCT TGATCCCTGC ACTGGAGTTT CCTTTTGCAC TAACCTCCTC CCTGTATGGA 
GATCCCCTCA GAGCCCAGCA CTTCATTTCT CTGAACATCC ACTGCCTTCT AAAGAATGGC 
AGTGACAATC TGTTTGAAGG CTTTGAACCA GAGACCTACT GGGATAGGAT GCAGTTCTTT 
CAGGAGGCCA TACTTTACAG GTTTGGATTT GTGCATGACA AATTTTCAGT TTCAACCTTT 
AGTTTCCCTT CGGAGAATAA GCCCCAGTAC ATCCACATAA CAGGTACCGT CTTCTTGCAG 
CTGCCATACT TCAAGAGGAA GCATTCAAGT GGGCAGCAAC GTCGACGCCG GAACTCTACC 
ACTTCGAACA ACCAGGGGCT GTTTGGTGGG GAGGAGTGTG TTGGCTACTA TTGGGCCTAC 
AACACTATGC TGACCAAGTC CTGGAGGGCA GGCGTGCTTG GGGATGAGAA GCTCGCCGAC 
CGCCTCCTCA GAGACTTCAG CGACTTCTGT GCCAACAAAG ACAACAGACT GTTAAACTTC 
TGGGACAGCT GTCAGGAGAA AATGAACACC AGCACTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

depdc5 ( XM_011616326.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011614628.1 Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo21242
Clone ID Related Accession (Same CDS sequence)	XM_011616326.1
Accession Version	XM_011616326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	DEP domain-containing protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071163.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG GTCCTGAAGA GCATAAAAGT 
GTAACATCAA ATGATAGTTT AGGCTCCGTG TCCAATGTGC TGCTCATTCC CCGTCTACCT 
TCAGCCCAGT ATGAAGTTAG CAGTTCCTTG GGATACACGA GCACCAGAGA AATGTTAGAG 
AAGATGGAGT CACAGAGGGA CTCCAGTGCA CCAGGAAGAT TTACAGTGGG GAGTGCTGAG 
TCCCCAAGGC ACATCCGTCC TGGATGCTAC ACCCCCCAAA GAGCACTCAT CAATCCTTTC 
ACCCCATCAA GGATGCCTAT GAAGCTAACC TCCAACCGCC GGCGTTGGAT GCACACCTTC 
CCCATAGGTC CCTCTGGAGA GGCAATTCAA ATCCATCATC AGATGAGACA GAACATGGCT 
GACCTGCAAG GCCAACAGAC AAATCCTGCA CATAGCTCAG CTGAGCTGTT GGAACTCGTC 
TGTCCTGAGG CCACTGGAAG AAAAACACTC TCAGGACAAG TGGGAGAAAC TGGCCTTTAT 
GTTGGGGGAT TGATGGAAGA ATTGACTGCA AATCCAGAGA GTAATGGTGG ACTTCCAACC 
CACTGTGCCT CCTTATTTGA TGACTGTCCC CTCAGCAGTG CTGACCCAAT GCCCAGCTTT 
TGTTGTACAG TGGGGGTGGA TTGGAAGTCT TTGACGACAC CAGCTTGTCT GCCCCTCACC 
ACGGACTATT TTCCAGATCG CCAAACCCTT CAGAATGATT ATACTGAAGG CTGCTATGAC 
CTGCTACCAC ACAGTGACCT GGACAGGCGG GAAGAAGAAG CAACTACTCA TAACAGGAAG 
CCTCAGCCAT CTGTGTCCAC ACCTCTTGGC AGCAGCCCTC TTTACTCCAG AGGCTTGGTT 
TCCCTGCGTC GAGCAGAAGA GAAGGAGAAT GTTTATTGGC TCAGCATGGG CCGTACCTTC 
CATAAAGTTT GCCTCAAAGA CAAAATAATC AGTGTAACTC GCTATTTGCC AAAGTACCCG 
TGTGAGACTG CTCAGATCCA GTACAGTTAC AGCCTTTGTC CATCACACTC AGAGGGTCAA 
TTTGTGTCCT GTTGGGTGGA GTTTGGTCAC GAACGGCTGG AAGAATACAA GTGGAACTAT 
CTGGACCAGT ACATCTGCTC TGCTGGGTCT GAGGACTTTA GTTTGATAGA CTCTCTAAAG 
TTTTGGAGGA CTCGGTTCTT GCTTCTGCCT GCTGGGGGAG CAAGGCGAAT GGCAGATGGT 
GAAGGGCACT GGGATATTTA TGGAGAGGCA GTGGGTACTG CAATGGGCGG ATCTGGGGAC 
TGGGTCTTGC TGGACAACTT CATCCGCTTC CTGGAGGGCC TGAACCGCAT TCGTCGCCGA 
CATCGCTCGG ACAGGATCAT TAGACAAAAG GGGACACCAA TGAAAGGCCT GCAGGTTGCT 
GGTACTCTCC CTTCTTATTC TTCTGAACAC ATGGCTCCTC CTCAAGGCAA AAAGGGCACA 
TCTGCATTAT CAGTCTTACT GGAGATGGAG CAGAAGACAC TGGATGAACA GCAGGGAACA 
AAGCCCTCAA CAGCTGTTGC TGACCCCTCT GGTATTGCAA CAAATCTCAT GTATCTGGAC 
AGTCCTCGCA AGGATGGTGG CTTCATTTTG GAATTTATTC GTAGCCCTCA TTCTTCCAAC 
ACCTATCACT CTCAGTTGCC TGTTGAAGCC AGTGAAGCGG CGGACAGAGG ACTCCAGCCA 
ACAGTTGCAC CTGGAGCAGC AGTCCAGCCT GTAGGAGAGC TTGCATCTTG TAGCAACACA 
GGAGACACCA GTGGAAATTC TGCAGTGCAG TCGCTTTCTT TGTCATCATC CCCAACACTC 
ATGGAGATCC TGGAGGCTAT AAAACATCCA ATGACAGGTG TACAACTCCT TCCAGAGCAA 
AGAGGCCTGC CTCTCAATTG CTTTTTTAGT GCGGAGGTGG TTCACTGGCT GATTAACAAC 
GTGGAAGGGG TAACGACACA AGGCATAGCT GTAGATATCA TGCAGAAAAT GTTGGATGAA 
GGTTTGGTGG CCCATGCTTC TGGAGACATG ATGCGTACCT TCATTTATGG ATTCTACTGC 
TACAGGATTG TTGGAGAGAA GGATGGTTCA AATTCCCAGC CTCCTTCCAC AACAGCAGGG 
GGCTGGCCTG CAGCAGCTCT GGAGGACTTT GCCCTGTTCC AGGGAAAGTG GTTTGAGGTG 
GCCTTTGTGG TGGAAGAGCA GCTCCATTGC GATCTCCCAG TTTTTCTCCT GCCCTGGCTC 
CCCAGCAGGC CGTCCTCCTA TGCAAGTAGG CATAGCTCAT TCAGCCGCAG TTTTGGAGGA 
CGCAGTCAGG CCGCTGCATT GCTAGCTGCC ACTGTTCCAG AGCAGAAAAT GGTCACTCTG 
GATGTGGATG TGAACAATCG CAGTGACCGG ATGGAGTGGT GCAGCTGTTA TTACCATGGC 
AACTTCTTCC TCAACACTGC CTTTGAGATC AAGCTTCACT GGATGGCTGT CACTGCTGCA 
GTACTCTTTG AGATGGTTCA GGGCTGGCAC AGGAAAGCAG CATCATGTGG CTTCCTCTTG 
ATCCCTGCAC TGGAGTTTCC TTTTGCACTA ACCTCCTCCC TGTATGGAGA TCCCCTCAGA 
GCCCAGCACT TCATTTCTCT GAACATCCAC TGCCTTCTAA AGAATGGCAG TGACAATCTG 
TTTGAAGGCT TTGAACCAGA GACCTACTGG GATAGGATGC AGTTCTTTCA GGAGGCCATA 
CTTTACAGGT TTGGATTTGT GCATGACAAA TTTTCAGTTT CAACCTTTAG TTTCCCTTCG 
GAGAATAAGC CCCAGTACAT CCACATAACA GGTACCGTCT TCTTGCAGCT GCCATACTTC 
AAGAGGAAGC ATTCAAGTGG GCAGCAACGT CGACGCCGGA ACTCTACCAC TTCGAACAAC 
CAGGGGCTGT TTGGTGGGGA GGAGTGTGTT GGCTACTATT GGGCCTACAA CACTATGCTG 
ACCAAGTCCT GGAGGGCAGG CGTGCTTGGG GATGAGAAGC TCGCCGACCG CCTCCTCAGA 
GACTTCAGCG ACTTCTGTGC CAACAAAGAC AACAGACTGT TAAACTTCTG GGACAGCTGT 
CAGGAGAAAA TGAACACCAG CACTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011614628.1
CDS	207..4856
Translation	MRTNKSYKLVLHKKGFGGSDDELVVNPKVFPQVCLRDIIEIAHPTDEYSPLLLQVKNLKEDLQKETISVDQTVAQAFKLRAYQDVIVNVINPKDVTLDLVELTFKDQYIGRGDMWRLKKCLVSTCAYVTQKVEFAGIRAQASELWLKGEKVTCGYISEDTRVVFRSTSAMVYIFIQMSSEMWDFDIYGDLYFEKAVNGFLSDLFAKWKEKNCSHEVTVVLFSRTFYNAKTVDEFPEVLRGSIRQDQECRFFEDFYRVVAQNERRDEWMSLLVSIKKLFVKYPLLVRLEETDGGPVGYNSTAAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRQLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRMMGVDSHVGDDYHLPHWINHSFYTSKSQTSCSSFTPRITVAGLKLTTEKSRFNKDHTLCSPKDTESSLPIQVDYDAHDIQVFRLPGPSRFKRTPNFRLGRDKEIGGRKSLGSLEVTACIGVSPPVRFEGPEEHKSVTSNDSLGSVSNVLLIPRLPSAQYEVSSSLGYTSTREMLEKMESQRDSSAPGRFTVGSAESPRHIRPGCYTPQRALINPFTPSRMPMKLTSNRRRWMHTFPIGPSGEAIQIHHQMRQNMADLQGQQTNPAHSSAELLELVCPEATGRKTLSGQVGETGLYVGGLMEELTANPESNGGLPTHCASLFDDCPLSSADPMPSFCCTVGVDWKSLTTPACLPLTTDYFPDRQTLQNDYTEGCYDLLPHSDLDRREEEATTHNRKPQPSVSTPLGSSPLYSRGLVSLRRAEEKENVYWLSMGRTFHKVCLKDKIISVTRYLPKYPCETAQIQYSYSLCPSHSEGQFVSCWVEFGHERLEEYKWNYLDQYICSAGSEDFSLIDSLKFWRTRFLLLPAGGARRMADGEGHWDIYGEAVGTAMGGSGDWVLLDNFIRFLEGLNRIRRRHRSDRIIRQKGTPMKGLQVAGTLPSYSSEHMAPPQGKKGTSALSVLLEMEQKTLDEQQGTKPSTAVADPSGIATNLMYLDSPRKDGGFILEFIRSPHSSNTYHSQLPVEASEAADRGLQPTVAPGAAVQPVGELASCSNTGDTSGNSAVQSLSLSSSPTLMEILEAIKHPMTGVQLLPEQRGLPLNCFFSAEVVHWLINNVEGVTTQGIAVDIMQKMLDEGLVAHASGDMMRTFIYGFYCYRIVGEKDGSNSQPPSTTAGGWPAAALEDFALFQGKWFEVAFVVEEQLHCDLPVFLLPWLPSRPSSYASRHSSFSRSFGGRSQAAALLAATVPEQKMVTLDVDVNNRSDRMEWCSCYYHGNFFLNTAFEIKLHWMAVTAAVLFEMVQGWHRKAASCGFLLIPALEFPFALTSSLYGDPLRAQHFISLNIHCLLKNGSDNLFEGFEPETYWDRMQFFQEAILYRFGFVHDKFSVSTFSFPSENKPQYIHITGTVFLQLPYFKRKHSSGQQRRRRNSTTSNNQGLFGGEECVGYYWAYNTMLTKSWRAGVLGDEKLADRLLRDFSDFCANKDNRLLNFWDSCQEKMNTSTP

Target ORF information:

RefSeq Version	XM_011616326.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011616326.1

ORF Insert Sequence:

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG GTCCTGAAGA GCATAAAAGT 
GTAACATCAA ATGATAGTTT AGGCTCCGTG TCCAATGTGC TGCTCATTCC CCGTCTACCT 
TCAGCCCAGT ATGAAGTTAG CAGTTCCTTG GGATACACGA GCACCAGAGA AATGTTAGAG 
AAGATGGAGT CACAGAGGGA CTCCAGTGCA CCAGGAAGAT TTACAGTGGG GAGTGCTGAG 
TCCCCAAGGC ACATCCGTCC TGGATGCTAC ACCCCCCAAA GAGCACTCAT CAATCCTTTC 
ACCCCATCAA GGATGCCTAT GAAGCTAACC TCCAACCGCC GGCGTTGGAT GCACACCTTC 
CCCATAGGTC CCTCTGGAGA GGCAATTCAA ATCCATCATC AGATGAGACA GAACATGGCT 
GACCTGCAAG GCCAACAGAC AAATCCTGCA CATAGCTCAG CTGAGCTGTT GGAACTCGTC 
TGTCCTGAGG CCACTGGAAG AAAAACACTC TCAGGACAAG TGGGAGAAAC TGGCCTTTAT 
GTTGGGGGAT TGATGGAAGA ATTGACTGCA AATCCAGAGA GTAATGGTGG ACTTCCAACC 
CACTGTGCCT CCTTATTTGA TGACTGTCCC CTCAGCAGTG CTGACCCAAT GCCCAGCTTT 
TGTTGTACAG TGGGGGTGGA TTGGAAGTCT TTGACGACAC CAGCTTGTCT GCCCCTCACC 
ACGGACTATT TTCCAGATCG CCAAACCCTT CAGAATGATT ATACTGAAGG CTGCTATGAC 
CTGCTACCAC ACAGTGACCT GGACAGGCGG GAAGAAGAAG CAACTACTCA TAACAGGAAG 
CCTCAGCCAT CTGTGTCCAC ACCTCTTGGC AGCAGCCCTC TTTACTCCAG AGGCTTGGTT 
TCCCTGCGTC GAGCAGAAGA GAAGGAGAAT GTTTATTGGC TCAGCATGGG CCGTACCTTC 
CATAAAGTTT GCCTCAAAGA CAAAATAATC AGTGTAACTC GCTATTTGCC AAAGTACCCG 
TGTGAGACTG CTCAGATCCA GTACAGTTAC AGCCTTTGTC CATCACACTC AGAGGGTCAA 
TTTGTGTCCT GTTGGGTGGA GTTTGGTCAC GAACGGCTGG AAGAATACAA GTGGAACTAT 
CTGGACCAGT ACATCTGCTC TGCTGGGTCT GAGGACTTTA GTTTGATAGA CTCTCTAAAG 
TTTTGGAGGA CTCGGTTCTT GCTTCTGCCT GCTGGGGGAG CAAGGCGAAT GGCAGATGGT 
GAAGGGCACT GGGATATTTA TGGAGAGGCA GTGGGTACTG CAATGGGCGG ATCTGGGGAC 
TGGGTCTTGC TGGACAACTT CATCCGCTTC CTGGAGGGCC TGAACCGCAT TCGTCGCCGA 
CATCGCTCGG ACAGGATCAT TAGACAAAAG GGGACACCAA TGAAAGGCCT GCAGGTTGCT 
GGTACTCTCC CTTCTTATTC TTCTGAACAC ATGGCTCCTC CTCAAGGCAA AAAGGGCACA 
TCTGCATTAT CAGTCTTACT GGAGATGGAG CAGAAGACAC TGGATGAACA GCAGGGAACA 
AAGCCCTCAA CAGCTGTTGC TGACCCCTCT GGTATTGCAA CAAATCTCAT GTATCTGGAC 
AGTCCTCGCA AGGATGGTGG CTTCATTTTG GAATTTATTC GTAGCCCTCA TTCTTCCAAC 
ACCTATCACT CTCAGTTGCC TGTTGAAGCC AGTGAAGCGG CGGACAGAGG ACTCCAGCCA 
ACAGTTGCAC CTGGAGCAGC AGTCCAGCCT GTAGGAGAGC TTGCATCTTG TAGCAACACA 
GGAGACACCA GTGGAAATTC TGCAGTGCAG TCGCTTTCTT TGTCATCATC CCCAACACTC 
ATGGAGATCC TGGAGGCTAT AAAACATCCA ATGACAGGTG TACAACTCCT TCCAGAGCAA 
AGAGGCCTGC CTCTCAATTG CTTTTTTAGT GCGGAGGTGG TTCACTGGCT GATTAACAAC 
GTGGAAGGGG TAACGACACA AGGCATAGCT GTAGATATCA TGCAGAAAAT GTTGGATGAA 
GGTTTGGTGG CCCATGCTTC TGGAGACATG ATGCGTACCT TCATTTATGG ATTCTACTGC 
TACAGGATTG TTGGAGAGAA GGATGGTTCA AATTCCCAGC CTCCTTCCAC AACAGCAGGG 
GGCTGGCCTG CAGCAGCTCT GGAGGACTTT GCCCTGTTCC AGGGAAAGTG GTTTGAGGTG 
GCCTTTGTGG TGGAAGAGCA GCTCCATTGC GATCTCCCAG TTTTTCTCCT GCCCTGGCTC 
CCCAGCAGGC CGTCCTCCTA TGCAAGTAGG CATAGCTCAT TCAGCCGCAG TTTTGGAGGA 
CGCAGTCAGG CCGCTGCATT GCTAGCTGCC ACTGTTCCAG AGCAGAAAAT GGTCACTCTG 
GATGTGGATG TGAACAATCG CAGTGACCGG ATGGAGTGGT GCAGCTGTTA TTACCATGGC 
AACTTCTTCC TCAACACTGC CTTTGAGATC AAGCTTCACT GGATGGCTGT CACTGCTGCA 
GTACTCTTTG AGATGGTTCA GGGCTGGCAC AGGAAAGCAG CATCATGTGG CTTCCTCTTG 
ATCCCTGCAC TGGAGTTTCC TTTTGCACTA ACCTCCTCCC TGTATGGAGA TCCCCTCAGA 
GCCCAGCACT TCATTTCTCT GAACATCCAC TGCCTTCTAA AGAATGGCAG TGACAATCTG 
TTTGAAGGCT TTGAACCAGA GACCTACTGG GATAGGATGC AGTTCTTTCA GGAGGCCATA 
CTTTACAGGT TTGGATTTGT GCATGACAAA TTTTCAGTTT CAACCTTTAG TTTCCCTTCG 
GAGAATAAGC CCCAGTACAT CCACATAACA GGTACCGTCT TCTTGCAGCT GCCATACTTC 
AAGAGGAAGC ATTCAAGTGG GCAGCAACGT CGACGCCGGA ACTCTACCAC TTCGAACAAC 
CAGGGGCTGT TTGGTGGGGA GGAGTGTGTT GGCTACTATT GGGCCTACAA CACTATGCTG 
ACCAAGTCCT GGAGGGCAGG CGTGCTTGGG GATGAGAAGC TCGCCGACCG CCTCCTCAGA 
GACTTCAGCG ACTTCTGTGC CAACAAAGAC AACAGACTGT TAAACTTCTG GGACAGCTGT 
CAGGAGAAAA TGAACACCAG CACTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

depdc5 ( XM_011616327.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011614629.1 Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OTo21243
Clone ID Related Accession (Same CDS sequence)	XM_011616327.1
Accession Version	XM_011616327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	DEP domain-containing protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071163.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG CCCAGTATGA AGTTAGCAGT 
TCCTTGGGAT ACACGAGCAC CAGAGAAATG TTAGAGAAGA TGGAGTCACA GAGGGACTCC 
AGTGCACCAG GAAGATTTAC AGTGGGGAGT GCTGAGTCCC CAAGGCACAT CCGTCCTGGA 
TGCTACACCC CCCAAAGAGC ACTCATCAAT CCTTTCACCC CATCAAGGAT GCCTATGAAG 
CTAACCTCCA ACCGCCGGCG TTGGATGCAC ACCTTCCCCA TAGGTCCCTC TGGAGAGGCA 
ATTCAAATCC ATCATCAGAT GAGACAGAAC ATGGCTGACC TGCAAGGCCA ACAGACAAAT 
CCTGCACATA GCTCAGCTGA GCTGTTGGAA CTCGTCTGTC CTGAGGCCAC TGGAAGAAAA 
ACACTCTCAG GACAAGTGGG AGAAACTGGC CTTTATGTTG GGGGATTGAT GGAAGAATTG 
ACTGCAAATC CAGAGAGTAA TGGTGGACTT CCAACCCACT GTGCCTCCTT ATTTGATGAC 
TGTCCCCTCA GCAGTGCTGA CCCAATGCCC AGCTTTTGTT GTACAGTGGG GGTGGATTGG 
AAGTCTTTGA CGACACCAGC TTGTCTGCCC CTCACCACGG ACTATTTTCC AGATCGCCAA 
ACCCTTCAGA ATGATTATAC TGAAGGCTGC TATGACCTGC TACCACACAG TGACCTGGAC 
AGGCGGGAAG AAGAAGCAAC TGTAAGAACA GCTTCCGAAG TGTTTGAGGA GTTTATCTGT 
CAGAGACTGA TGCAGGGGTA TCAAATCATT GTTCAGACTC ATAACAGGAA GCCTCAGCCA 
TCTGTGTCCA CACCTCTTGG CAGCAGCCCT CTTTACTCCA GAGGCTTGGT TTCCCTGCGT 
CGAGCAGAAG AGAAGGAGAA TGTTTATTGG CTCAGCATGG GCCGTACCTT CCATAAAGTT 
TGCCTCAAAG ACAAAATAAT CAGTGTAACT CGCTATTTGC CAAAGTACCC GTGTGAGACT 
GCTCAGATCC AGTACAGTTA CAGCCTTTGT CCATCACACT CAGAGGGTCA ATTTGTGTCC 
TGTTGGGTGG AGTTTGGTCA CGAACGGCTG GAAGAATACA AGTGGAACTA TCTGGACCAG 
TACATCTGCT CTGCTGGGTC TGAGGACTTT AGTTTGATAG ACTCTCTAAA GTTTTGGAGG 
ACTCGGTTCT TGCTTCTGCC TGCTGGGGGA GCAAGGCGAA TGGCAGATGG TGAAGGGCAC 
TGGGATATTT ATGGAGAGGC AGTGGGTACT GCAATGGGCG GATCTGGGGA CTGGGTCTTG 
CTGGACAACT TCATCCGCTT CCTGGAGGGC CTGAACCGCA TTCGTCGCCG ACATCGCTCG 
GACAGGATCA TTAGACAAAA GGGGACACCA ATGAAAGGCC TGCAGGTTGC TGGTACTCTC 
CCTTCTTATT CTTCTGAACA CATGGCTCCT CCTCAAGGCA AAAAGGGCAC ATCTGCATTA 
TCAGTCTTAC TGGAGATGGA GCAGAAGACA CTGGATGAAC AGCAGGGAAC AAAGCCCTCA 
ACAGCTGTTG CTGACCCCTC TGGTATTGCA ACAAATCTCA TGTATCTGGA CAGTCCTCGC 
AAGGATGGTG GCTTCATTTT GGAATTTATT CGTAGCCCTC ATTCTTCCAA CACCTATCAC 
TCTCAGTTGC CTGTTGAAGC CAGTGAAGCG GCGGACAGAG GACTCCAGCC AACAGTTGCA 
CCTGGAGCAG CAGTCCAGCC TGTAGGAGAG CTTGCATCTT GTAGCAACAC AGGAGACACC 
AGTGGAAATT CTGCAGTGCA GTCGCTTTCT TTGTCATCAT CCCCAACACT CATGGAGATC 
CTGGAGGCTA TAAAACATCC AATGACAGGT GTACAACTCC TTCCAGAGCA AAGAGGCCTG 
CCTCTCAATT GCTTTTTTAG TGCGGAGGTG GTTCACTGGC TGATTAACAA CGTGGAAGGG 
GTAACGACAC AAGGCATAGC TGTAGATATC ATGCAGAAAA TGTTGGATGA AGGTTTGGTG 
GCCCATGCTT CTGGAGACAT GATGCGTACC TTCATTTATG GATTCTACTG CTACAGGATT 
GTTGGAGAGA AGGATGGTTC AAATTCCCAG CCTCCTTCCA CAACAGCAGG GGGCTGGCCT 
GCAGCAGCTC TGGAGGACTT TGCCCTGTTC CAGGGAAAGT GGTTTGAGGT GGCCTTTGTG 
GTGGAAGAGC AGCTCCATTG CGATCTCCCA GTTTTTCTCC TGCCCTGGCT CCCCAGCAGG 
CCGTCCTCCT ATGCAAGTAG GCATAGCTCA TTCAGCCGCA GTTTTGGAGG ACGCAGTCAG 
GCCGCTGCAT TGCTAGCTGC CACTGTTCCA GAGCAGAAAA TGGTCACTCT GGATGTGGAT 
GTGAACAATC GCAGTGACCG GATGGAGTGG TGCAGCTGTT ATTACCATGG CAACTTCTTC 
CTCAACACTG CCTTTGAGAT CAAGCTTCAC TGGATGGCTG TCACTGCTGC AGTACTCTTT 
GAGATGGTTC AGGGCTGGCA CAGGAAAGCA GCATCATGTG GCTTCCTCTT GATCCCTGCA 
CTGGAGTTTC CTTTTGCACT AACCTCCTCC CTGTATGGAG ATCCCCTCAG AGCCCAGCAC 
TTCATTTCTC TGAACATCCA CTGCCTTCTA AAGAATGGCA GTGACAATCT GTTTGAAGGC 
TTTGAACCAG AGACCTACTG GGATAGGATG CAGTTCTTTC AGGAGGCCAT ACTTTACAGG 
TTTGGATTTG TGCATGACAA ATTTTCAGTT TCAACCTTTA GTTTCCCTTC GGAGAATAAG 
CCCCAGTACA TCCACATAAC AGGTACCGTC TTCTTGCAGC TGCCATACTT CAAGAGGAAG 
CATTCAAGTG GGCAGCAACG TCGACGCCGG AACTCTACCA CTTCGAACAA CCAGGGGCTG 
TTTGGTGGGG AGGAGTGTGT TGGCTACTAT TGGGCCTACA ACACTATGCT GACCAAGTCC 
TGGAGGGCAG GCGTGCTTGG GGATGAGAAG CTCGCCGACC GCCTCCTCAG AGACTTCAGC 
GACTTCTGTG CCAACAAAGA CAACAGACTG TTAAACTTCT GGGACAGCTG TCAGGAGAAA 
ATGAACACCA GCACTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011614629.1
CDS	207..4847
Translation	MRTNKSYKLVLHKKGFGGSDDELVVNPKVFPQVCLRDIIEIAHPTDEYSPLLLQVKNLKEDLQKETISVDQTVAQAFKLRAYQDVIVNVINPKDVTLDLVELTFKDQYIGRGDMWRLKKCLVSTCAYVTQKVEFAGIRAQASELWLKGEKVTCGYISEDTRVVFRSTSAMVYIFIQMSSEMWDFDIYGDLYFEKAVNGFLSDLFAKWKEKNCSHEVTVVLFSRTFYNAKTVDEFPEVLRGSIRQDQECRFFEDFYRVVAQNERRDEWMSLLVSIKKLFVKYPLLVRLEETDGGPVGYNSTAAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRQLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRMMGVDSHVGDDYHLPHWINHSFYTSKSQTSCSSFTPRITVAGLKLTTEKSRFNKDHTLCSPKDTESSLPIQVDYDAHDIQVFRLPGPSRFKRTPNFRLGRDKEIGGRKSLGSLEVTACIGVSPPVRFEAQYEVSSSLGYTSTREMLEKMESQRDSSAPGRFTVGSAESPRHIRPGCYTPQRALINPFTPSRMPMKLTSNRRRWMHTFPIGPSGEAIQIHHQMRQNMADLQGQQTNPAHSSAELLELVCPEATGRKTLSGQVGETGLYVGGLMEELTANPESNGGLPTHCASLFDDCPLSSADPMPSFCCTVGVDWKSLTTPACLPLTTDYFPDRQTLQNDYTEGCYDLLPHSDLDRREEEATVRTASEVFEEFICQRLMQGYQIIVQTHNRKPQPSVSTPLGSSPLYSRGLVSLRRAEEKENVYWLSMGRTFHKVCLKDKIISVTRYLPKYPCETAQIQYSYSLCPSHSEGQFVSCWVEFGHERLEEYKWNYLDQYICSAGSEDFSLIDSLKFWRTRFLLLPAGGARRMADGEGHWDIYGEAVGTAMGGSGDWVLLDNFIRFLEGLNRIRRRHRSDRIIRQKGTPMKGLQVAGTLPSYSSEHMAPPQGKKGTSALSVLLEMEQKTLDEQQGTKPSTAVADPSGIATNLMYLDSPRKDGGFILEFIRSPHSSNTYHSQLPVEASEAADRGLQPTVAPGAAVQPVGELASCSNTGDTSGNSAVQSLSLSSSPTLMEILEAIKHPMTGVQLLPEQRGLPLNCFFSAEVVHWLINNVEGVTTQGIAVDIMQKMLDEGLVAHASGDMMRTFIYGFYCYRIVGEKDGSNSQPPSTTAGGWPAAALEDFALFQGKWFEVAFVVEEQLHCDLPVFLLPWLPSRPSSYASRHSSFSRSFGGRSQAAALLAATVPEQKMVTLDVDVNNRSDRMEWCSCYYHGNFFLNTAFEIKLHWMAVTAAVLFEMVQGWHRKAASCGFLLIPALEFPFALTSSLYGDPLRAQHFISLNIHCLLKNGSDNLFEGFEPETYWDRMQFFQEAILYRFGFVHDKFSVSTFSFPSENKPQYIHITGTVFLQLPYFKRKHSSGQQRRRRNSTTSNNQGLFGGEECVGYYWAYNTMLTKSWRAGVLGDEKLADRLLRDFSDFCANKDNRLLNFWDSCQEKMNTSTP

Target ORF information:

RefSeq Version	XM_011616327.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011616327.1

ORF Insert Sequence:

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG CCCAGTATGA AGTTAGCAGT 
TCCTTGGGAT ACACGAGCAC CAGAGAAATG TTAGAGAAGA TGGAGTCACA GAGGGACTCC 
AGTGCACCAG GAAGATTTAC AGTGGGGAGT GCTGAGTCCC CAAGGCACAT CCGTCCTGGA 
TGCTACACCC CCCAAAGAGC ACTCATCAAT CCTTTCACCC CATCAAGGAT GCCTATGAAG 
CTAACCTCCA ACCGCCGGCG TTGGATGCAC ACCTTCCCCA TAGGTCCCTC TGGAGAGGCA 
ATTCAAATCC ATCATCAGAT GAGACAGAAC ATGGCTGACC TGCAAGGCCA ACAGACAAAT 
CCTGCACATA GCTCAGCTGA GCTGTTGGAA CTCGTCTGTC CTGAGGCCAC TGGAAGAAAA 
ACACTCTCAG GACAAGTGGG AGAAACTGGC CTTTATGTTG GGGGATTGAT GGAAGAATTG 
ACTGCAAATC CAGAGAGTAA TGGTGGACTT CCAACCCACT GTGCCTCCTT ATTTGATGAC 
TGTCCCCTCA GCAGTGCTGA CCCAATGCCC AGCTTTTGTT GTACAGTGGG GGTGGATTGG 
AAGTCTTTGA CGACACCAGC TTGTCTGCCC CTCACCACGG ACTATTTTCC AGATCGCCAA 
ACCCTTCAGA ATGATTATAC TGAAGGCTGC TATGACCTGC TACCACACAG TGACCTGGAC 
AGGCGGGAAG AAGAAGCAAC TGTAAGAACA GCTTCCGAAG TGTTTGAGGA GTTTATCTGT 
CAGAGACTGA TGCAGGGGTA TCAAATCATT GTTCAGACTC ATAACAGGAA GCCTCAGCCA 
TCTGTGTCCA CACCTCTTGG CAGCAGCCCT CTTTACTCCA GAGGCTTGGT TTCCCTGCGT 
CGAGCAGAAG AGAAGGAGAA TGTTTATTGG CTCAGCATGG GCCGTACCTT CCATAAAGTT 
TGCCTCAAAG ACAAAATAAT CAGTGTAACT CGCTATTTGC CAAAGTACCC GTGTGAGACT 
GCTCAGATCC AGTACAGTTA CAGCCTTTGT CCATCACACT CAGAGGGTCA ATTTGTGTCC 
TGTTGGGTGG AGTTTGGTCA CGAACGGCTG GAAGAATACA AGTGGAACTA TCTGGACCAG 
TACATCTGCT CTGCTGGGTC TGAGGACTTT AGTTTGATAG ACTCTCTAAA GTTTTGGAGG 
ACTCGGTTCT TGCTTCTGCC TGCTGGGGGA GCAAGGCGAA TGGCAGATGG TGAAGGGCAC 
TGGGATATTT ATGGAGAGGC AGTGGGTACT GCAATGGGCG GATCTGGGGA CTGGGTCTTG 
CTGGACAACT TCATCCGCTT CCTGGAGGGC CTGAACCGCA TTCGTCGCCG ACATCGCTCG 
GACAGGATCA TTAGACAAAA GGGGACACCA ATGAAAGGCC TGCAGGTTGC TGGTACTCTC 
CCTTCTTATT CTTCTGAACA CATGGCTCCT CCTCAAGGCA AAAAGGGCAC ATCTGCATTA 
TCAGTCTTAC TGGAGATGGA GCAGAAGACA CTGGATGAAC AGCAGGGAAC AAAGCCCTCA 
ACAGCTGTTG CTGACCCCTC TGGTATTGCA ACAAATCTCA TGTATCTGGA CAGTCCTCGC 
AAGGATGGTG GCTTCATTTT GGAATTTATT CGTAGCCCTC ATTCTTCCAA CACCTATCAC 
TCTCAGTTGC CTGTTGAAGC CAGTGAAGCG GCGGACAGAG GACTCCAGCC AACAGTTGCA 
CCTGGAGCAG CAGTCCAGCC TGTAGGAGAG CTTGCATCTT GTAGCAACAC AGGAGACACC 
AGTGGAAATT CTGCAGTGCA GTCGCTTTCT TTGTCATCAT CCCCAACACT CATGGAGATC 
CTGGAGGCTA TAAAACATCC AATGACAGGT GTACAACTCC TTCCAGAGCA AAGAGGCCTG 
CCTCTCAATT GCTTTTTTAG TGCGGAGGTG GTTCACTGGC TGATTAACAA CGTGGAAGGG 
GTAACGACAC AAGGCATAGC TGTAGATATC ATGCAGAAAA TGTTGGATGA AGGTTTGGTG 
GCCCATGCTT CTGGAGACAT GATGCGTACC TTCATTTATG GATTCTACTG CTACAGGATT 
GTTGGAGAGA AGGATGGTTC AAATTCCCAG CCTCCTTCCA CAACAGCAGG GGGCTGGCCT 
GCAGCAGCTC TGGAGGACTT TGCCCTGTTC CAGGGAAAGT GGTTTGAGGT GGCCTTTGTG 
GTGGAAGAGC AGCTCCATTG CGATCTCCCA GTTTTTCTCC TGCCCTGGCT CCCCAGCAGG 
CCGTCCTCCT ATGCAAGTAG GCATAGCTCA TTCAGCCGCA GTTTTGGAGG ACGCAGTCAG 
GCCGCTGCAT TGCTAGCTGC CACTGTTCCA GAGCAGAAAA TGGTCACTCT GGATGTGGAT 
GTGAACAATC GCAGTGACCG GATGGAGTGG TGCAGCTGTT ATTACCATGG CAACTTCTTC 
CTCAACACTG CCTTTGAGAT CAAGCTTCAC TGGATGGCTG TCACTGCTGC AGTACTCTTT 
GAGATGGTTC AGGGCTGGCA CAGGAAAGCA GCATCATGTG GCTTCCTCTT GATCCCTGCA 
CTGGAGTTTC CTTTTGCACT AACCTCCTCC CTGTATGGAG ATCCCCTCAG AGCCCAGCAC 
TTCATTTCTC TGAACATCCA CTGCCTTCTA AAGAATGGCA GTGACAATCT GTTTGAAGGC 
TTTGAACCAG AGACCTACTG GGATAGGATG CAGTTCTTTC AGGAGGCCAT ACTTTACAGG 
TTTGGATTTG TGCATGACAA ATTTTCAGTT TCAACCTTTA GTTTCCCTTC GGAGAATAAG 
CCCCAGTACA TCCACATAAC AGGTACCGTC TTCTTGCAGC TGCCATACTT CAAGAGGAAG 
CATTCAAGTG GGCAGCAACG TCGACGCCGG AACTCTACCA CTTCGAACAA CCAGGGGCTG 
TTTGGTGGGG AGGAGTGTGT TGGCTACTAT TGGGCCTACA ACACTATGCT GACCAAGTCC 
TGGAGGGCAG GCGTGCTTGG GGATGAGAAG CTCGCCGACC GCCTCCTCAG AGACTTCAGC 
GACTTCTGTG CCAACAAAGA CAACAGACTG TTAAACTTCT GGGACAGCTG TCAGGAGAAA 
ATGAACACCA GCACTCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

depdc5 ( XM_011616328.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011614630.1 Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X6, mRNA.

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CloneID	OTo21244
Clone ID Related Accession (Same CDS sequence)	XM_011616328.1
Accession Version	XM_011616328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	DEP domain-containing protein 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071163.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATAG AGACAATCAG TGTTGACCAG ACTGTAGCAC AAGCCTTCAA ACTACGGGCT 
TACCAGGATG TCATTGTCAA CGTTATCAAT CCAAAGGATG TAACACTAGA CCTGGTGGAA 
CTGACGTTCA AAGATCAATA CATTGGCAGA GGAGACATGT GGAGATTAAA AAAGTGTTTG 
GTAAGCACCT GTGCTTATGT GACACAGAAG GTGGAATTTG CAGGAATCAG AGCCCAGGCC 
AGTGAACTGT GGTTAAAGGG TGAAAAAGTG ACCTGTGGGT ACATCAGTGA GGACACCCGG 
GTGGTGTTTC GCTCCACATC TGCCATGGTT TACATCTTTA TCCAGATGAG TAGTGAGATG 
TGGGACTTTG ATATTTATGG TGATCTCTAC TTTGAGAAAG CTGTGAATGG ATTCCTCTCT 
GATCTTTTTG CCAAATGGAA GGAAAAGAAT TGCAGCCATG AGGTGACAGT TGTTCTTTTC 
TCACGTACAT TCTACAATGC CAAAACAGTT GATGAATTTC CTGAAGTTCT CAGAGGGTCA 
ATTAGGCAGG ACCAGGAATG CCGTTTTTTT GAAGACTTTT ACAGGGTTGT TGCTCAGAAT 
GAGAGGCGTG ATGAGTGGAT GTCATTGCTG GTCTCTATAA AGAAGCTTTT TGTTAAGTAT 
CCATTGCTGG TGCGGCTTGA AGAAACAGAT GGTGGCCCGG TTGGTTATAA CTCTACTGCA 
GCTCAAGGAA ACTATCTGGA AGCCATTAAT CTTTCCTTCA ATGTGTTTGA CAAGCATTAC 
ATCAACCGTA ACTTTGACCG CACTGGCCAG ATGTCGGTGG TCATCACACC TGGGGTTGGA 
GTGTTTGAAG TTGACCGCCA GCTCATGATT CTTACCAAAC AGCGTATGAT TGATAATGGT 
ATCGGGGTGG ACTTGGTGTG TATGGGGGAA CAGCCTCTAC ATGCAGTACC ATTATTCAAG 
CTGCACAACA GGATGATGGG TGTGGACTCT CATGTAGGAG ATGATTATCA CCTTCCTCAC 
TGGATCAACC ACAGCTTCTA TACCTCCAAA AGTCAAACCT CTTGTAGCTC CTTCACCCCT 
CGAATCACAG TGGCTGGACT CAAGCTCACT ACAGAGAAGT CCAGATTCAA CAAGGACCAC 
ACTCTCTGTT CTCCAAAGGA CACTGAAAGC AGTCTGCCTA TACAGGTGGA CTACGATGCC 
CATGATATCC AGGTGTTCAG ATTACCTGGT CCATCACGAT TTAAGAGGAC TCCTAATTTT 
AGGTTAGGGC GAGACAAAGA GATAGGTGGA AGAAAAAGTT TGGGCTCCCT GGAAGTGACT 
GCATGCATAG GGGTATCCCC CCCTGTTCGG TTTGAAGGTC CTGAAGAGCA TAAAAGTGTA 
ACATCAAATG ATAGTTTAGG CTCCGTGTCC AATGTGCTGC TCATTCCCCG TCTACCTTCA 
GCCCAGTATG AAGTTAGCAG TTCCTTGGGA TACACGAGCA CCAGAGAAAT GTTAGAGAAG 
ATGGAGTCAC AGAGGGACTC CAGTGCACCA GGAAGATTTA CAGTGGGGAG TGCTGAGTCC 
CCAAGGCACA TCCGTCCTGG ATGCTACACC CCCCAAAGAG CACTCATCAA TCCTTTCACC 
CCATCAAGGA TGCCTATGAA GCTAACCTCC AACCGCCGGC GTTGGATGCA CACCTTCCCC 
ATAGGTCCCT CTGGAGAGGC AATTCAAATC CATCATCAGA TGAGACAGAA CATGGCTGAC 
CTGCAAGGCC AACAGACAAA TCCTGCACAT AGCTCAGCTG AGCTGTTGGA ACTCGTCTGT 
CCTGAGGCCA CTGGAAGAAA AACACTCTCA GGACAAGTGG GAGAAACTGG CCTTTATGTT 
GGGGGATTGA TGGAAGAATT GACTGCAAAT CCAGAGAGTA ATGGTGGACT TCCAACCCAC 
TGTGCCTCCT TATTTGATGA CTGTCCCCTC AGCAGTGCTG ACCCAATGCC CAGCTTTTGT 
TGTACAGTGG GGGTGGATTG GAAGTCTTTG ACGACACCAG CTTGTCTGCC CCTCACCACG 
GACTATTTTC CAGATCGCCA AACCCTTCAG AATGATTATA CTGAAGGCTG CTATGACCTG 
CTACCACACA GTGACCTGGA CAGGCGGGAA GAAGAAGCAA CTGTAAGAAC AGCTTCCGAA 
GTGTTTGAGG AGTTTATCTG TCAGAGACTG ATGCAGGGGT ATCAAATCAT TGTTCAGACT 
CATAACAGGA AGCCTCAGCC ATCTGTGTCC ACACCTCTTG GCAGCAGCCC TCTTTACTCC 
AGAGGCTTGG TTTCCCTGCG TCGAGCAGAA GAGAAGGAGA ATGTTTATTG GCTCAGCATG 
GGCCGTACCT TCCATAAAGT TTGCCTCAAA GACAAAATAA TCAGTGTAAC TCGCTATTTG 
CCAAAGTACC CGTGTGAGAC TGCTCAGATC CAGTACAGTT ACAGCCTTTG TCCATCACAC 
TCAGAGGGTC AATTTGTGTC CTGTTGGGTG GAGTTTGGTC ACGAACGGCT GGAAGAATAC 
AAGTGGAACT ATCTGGACCA GTACATCTGC TCTGCTGGGT CTGAGGACTT TAGTTTGATA 
GACTCTCTAA AGTTTTGGAG GACTCGGTTC TTGCTTCTGC CTGCTGGGGG AGCAAGGCGA 
ATGGCAGATG GTGAAGGGCA CTGGGATATT TATGGAGAGG CAGTGGGTAC TGCAATGGGC 
GGATCTGGGG ACTGGGTCTT GCTGGACAAC TTCATCCGCT TCCTGGAGGG CCTGAACCGC 
ATTCGTCGCC GACATCGCTC GGACAGGATC ATTAGACAAA AGGGGACACC AATGAAAGGC 
CTGCAGGTTG CTGGTACTCT CCCTTCTTAT TCTTCTGAAC ACATGGCTCC TCCTCAAGGC 
AAAAAGGGCA CATCTGCATT ATCAGTCTTA CTGGAGATGG AGCAGAAGAC ACTGGATGAA 
CAGCAGGGAA CAAAGCCCTC AACAGCTGTT GCTGACCCCT CTGGTATTGC AACAAATCTC 
ATGTATCTGG ACAGTCCTCG CAAGGATGGT GGCTTCATTT TGGAATTTAT TCGTAGCCCT 
CATTCTTCCA ACACCTATCA CTCTCAGTTG CCTGTTGAAG CCAGTGAAGC GGCGGACAGA 
GGACTCCAGC CAACAGTTGC ACCTGGAGCA GCAGTCCAGC CTGTAGGAGA GCTTGCATCT 
TGTAGCAACA CAGGAGACAC CAGTGGAAAT TCTGCAGTGC AGTCGCTTTC TTTGTCATCA 
TCCCCAACAC TCATGGAGAT CCTGGAGGCT ATAAAACATC CAATGACAGG TGTACAACTC 
CTTCCAGAGC AAAGAGGCCT GCCTCTCAAT TGCTTTTTTA GTGCGGAGGT GGTTCACTGG 
CTGATTAACA ACGTGGAAGG GGTAACGACA CAAGGCATAG CTGTAGATAT CATGCAGAAA 
ATGTTGGATG AAGGTTTGGT GGCCCATGCT TCTGGAGACA TGATGCGTAC CTTCATTTAT 
GGATTCTACT GCTACAGGAT TGTTGGAGAG AAGGATGGTT CAAATTCCCA GCCTCCTTCC 
ACAACAGCAG GGGGCTGGCC TGCAGCAGCT CTGGAGGACT TTGCCCTGTT CCAGGGAAAG 
TGGTTTGAGG TGGCCTTTGT GGTGGAAGAG CAGCTCCATT GCGATCTCCC AGTTTTTCTC 
CTGCCCTGGC TCCCCAGCAG GCCGTCCTCC TATGCAAGTA GGCATAGCTC ATTCAGCCGC 
AGTTTTGGAG GACGCAGTCA GGCCGCTGCA TTGCTAGCTG CCACTGTTCC AGAGCAGAAA 
ATGGTCACTC TGGATGTGGA TGTGAACAAT CGCAGTGACC GGATGGAGTG GTGCAGCTGT 
TATTACCATG GCAACTTCTT CCTCAACACT GCCTTTGAGA TCAAGCTTCA CTGGATGGCT 
GTCACTGCTG CAGTACTCTT TGAGATGGTT CAGGGCTGGC ACAGGAAAGC AGCATCATGT 
GGCTTCCTCT TGATCCCTGC ACTGGAGTTT CCTTTTGCAC TAACCTCCTC CCTGTATGGA 
GATCCCCTCA GAGCCCAGCA CTTCATTTCT CTGAACATCC ACTGCCTTCT AAAGAATGGC 
AGTGACAATC TGTTTGAAGG CTTTGAACCA GAGACCTACT GGGATAGGAT GCAGTTCTTT 
CAGGAGGCCA TACTTTACAG GTTTGGATTT GTGCATGACA AATTTTCAGT TTCAACCTTT 
AGTTTCCCTT CGGAGAATAA GCCCCAGTAC ATCCACATAA CAGGTACCGT CTTCTTGCAG 
CTGCCATACT TCAAGAGGAA GCATTCAAGT GGGCAGCAAC GTCGACGCCG GAACTCTACC 
ACTTCGAACA ACCAGGGGCT GTTTGGTGGG GAGGAGTGTG TTGGCTACTA TTGGGCCTAC 
AACACTATGC TGACCAAGTC CTGGAGGGCA GGCGTGCTTG GGGATGAGAA GCTCGCCGAC 
CGCCTCCTCA GAGACTTCAG CGACTTCTGT GCCAACAAAG ACAACAGACT GTTAAACTTC 
TGGGACAGCT GTCAGGAGAA AATGAACACC AGCACTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011614630.1
CDS	79..4620
Translation	MNIETISVDQTVAQAFKLRAYQDVIVNVINPKDVTLDLVELTFKDQYIGRGDMWRLKKCLVSTCAYVTQKVEFAGIRAQASELWLKGEKVTCGYISEDTRVVFRSTSAMVYIFIQMSSEMWDFDIYGDLYFEKAVNGFLSDLFAKWKEKNCSHEVTVVLFSRTFYNAKTVDEFPEVLRGSIRQDQECRFFEDFYRVVAQNERRDEWMSLLVSIKKLFVKYPLLVRLEETDGGPVGYNSTAAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRQLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRMMGVDSHVGDDYHLPHWINHSFYTSKSQTSCSSFTPRITVAGLKLTTEKSRFNKDHTLCSPKDTESSLPIQVDYDAHDIQVFRLPGPSRFKRTPNFRLGRDKEIGGRKSLGSLEVTACIGVSPPVRFEGPEEHKSVTSNDSLGSVSNVLLIPRLPSAQYEVSSSLGYTSTREMLEKMESQRDSSAPGRFTVGSAESPRHIRPGCYTPQRALINPFTPSRMPMKLTSNRRRWMHTFPIGPSGEAIQIHHQMRQNMADLQGQQTNPAHSSAELLELVCPEATGRKTLSGQVGETGLYVGGLMEELTANPESNGGLPTHCASLFDDCPLSSADPMPSFCCTVGVDWKSLTTPACLPLTTDYFPDRQTLQNDYTEGCYDLLPHSDLDRREEEATVRTASEVFEEFICQRLMQGYQIIVQTHNRKPQPSVSTPLGSSPLYSRGLVSLRRAEEKENVYWLSMGRTFHKVCLKDKIISVTRYLPKYPCETAQIQYSYSLCPSHSEGQFVSCWVEFGHERLEEYKWNYLDQYICSAGSEDFSLIDSLKFWRTRFLLLPAGGARRMADGEGHWDIYGEAVGTAMGGSGDWVLLDNFIRFLEGLNRIRRRHRSDRIIRQKGTPMKGLQVAGTLPSYSSEHMAPPQGKKGTSALSVLLEMEQKTLDEQQGTKPSTAVADPSGIATNLMYLDSPRKDGGFILEFIRSPHSSNTYHSQLPVEASEAADRGLQPTVAPGAAVQPVGELASCSNTGDTSGNSAVQSLSLSSSPTLMEILEAIKHPMTGVQLLPEQRGLPLNCFFSAEVVHWLINNVEGVTTQGIAVDIMQKMLDEGLVAHASGDMMRTFIYGFYCYRIVGEKDGSNSQPPSTTAGGWPAAALEDFALFQGKWFEVAFVVEEQLHCDLPVFLLPWLPSRPSSYASRHSSFSRSFGGRSQAAALLAATVPEQKMVTLDVDVNNRSDRMEWCSCYYHGNFFLNTAFEIKLHWMAVTAAVLFEMVQGWHRKAASCGFLLIPALEFPFALTSSLYGDPLRAQHFISLNIHCLLKNGSDNLFEGFEPETYWDRMQFFQEAILYRFGFVHDKFSVSTFSFPSENKPQYIHITGTVFLQLPYFKRKHSSGQQRRRRNSTTSNNQGLFGGEECVGYYWAYNTMLTKSWRAGVLGDEKLADRLLRDFSDFCANKDNRLLNFWDSCQEKMNTSTP

Target ORF information:

RefSeq Version	XM_011616328.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011616328.1

ORF Insert Sequence:

ATGAATATAG AGACAATCAG TGTTGACCAG ACTGTAGCAC AAGCCTTCAA ACTACGGGCT 
TACCAGGATG TCATTGTCAA CGTTATCAAT CCAAAGGATG TAACACTAGA CCTGGTGGAA 
CTGACGTTCA AAGATCAATA CATTGGCAGA GGAGACATGT GGAGATTAAA AAAGTGTTTG 
GTAAGCACCT GTGCTTATGT GACACAGAAG GTGGAATTTG CAGGAATCAG AGCCCAGGCC 
AGTGAACTGT GGTTAAAGGG TGAAAAAGTG ACCTGTGGGT ACATCAGTGA GGACACCCGG 
GTGGTGTTTC GCTCCACATC TGCCATGGTT TACATCTTTA TCCAGATGAG TAGTGAGATG 
TGGGACTTTG ATATTTATGG TGATCTCTAC TTTGAGAAAG CTGTGAATGG ATTCCTCTCT 
GATCTTTTTG CCAAATGGAA GGAAAAGAAT TGCAGCCATG AGGTGACAGT TGTTCTTTTC 
TCACGTACAT TCTACAATGC CAAAACAGTT GATGAATTTC CTGAAGTTCT CAGAGGGTCA 
ATTAGGCAGG ACCAGGAATG CCGTTTTTTT GAAGACTTTT ACAGGGTTGT TGCTCAGAAT 
GAGAGGCGTG ATGAGTGGAT GTCATTGCTG GTCTCTATAA AGAAGCTTTT TGTTAAGTAT 
CCATTGCTGG TGCGGCTTGA AGAAACAGAT GGTGGCCCGG TTGGTTATAA CTCTACTGCA 
GCTCAAGGAA ACTATCTGGA AGCCATTAAT CTTTCCTTCA ATGTGTTTGA CAAGCATTAC 
ATCAACCGTA ACTTTGACCG CACTGGCCAG ATGTCGGTGG TCATCACACC TGGGGTTGGA 
GTGTTTGAAG TTGACCGCCA GCTCATGATT CTTACCAAAC AGCGTATGAT TGATAATGGT 
ATCGGGGTGG ACTTGGTGTG TATGGGGGAA CAGCCTCTAC ATGCAGTACC ATTATTCAAG 
CTGCACAACA GGATGATGGG TGTGGACTCT CATGTAGGAG ATGATTATCA CCTTCCTCAC 
TGGATCAACC ACAGCTTCTA TACCTCCAAA AGTCAAACCT CTTGTAGCTC CTTCACCCCT 
CGAATCACAG TGGCTGGACT CAAGCTCACT ACAGAGAAGT CCAGATTCAA CAAGGACCAC 
ACTCTCTGTT CTCCAAAGGA CACTGAAAGC AGTCTGCCTA TACAGGTGGA CTACGATGCC 
CATGATATCC AGGTGTTCAG ATTACCTGGT CCATCACGAT TTAAGAGGAC TCCTAATTTT 
AGGTTAGGGC GAGACAAAGA GATAGGTGGA AGAAAAAGTT TGGGCTCCCT GGAAGTGACT 
GCATGCATAG GGGTATCCCC CCCTGTTCGG TTTGAAGGTC CTGAAGAGCA TAAAAGTGTA 
ACATCAAATG ATAGTTTAGG CTCCGTGTCC AATGTGCTGC TCATTCCCCG TCTACCTTCA 
GCCCAGTATG AAGTTAGCAG TTCCTTGGGA TACACGAGCA CCAGAGAAAT GTTAGAGAAG 
ATGGAGTCAC AGAGGGACTC CAGTGCACCA GGAAGATTTA CAGTGGGGAG TGCTGAGTCC 
CCAAGGCACA TCCGTCCTGG ATGCTACACC CCCCAAAGAG CACTCATCAA TCCTTTCACC 
CCATCAAGGA TGCCTATGAA GCTAACCTCC AACCGCCGGC GTTGGATGCA CACCTTCCCC 
ATAGGTCCCT CTGGAGAGGC AATTCAAATC CATCATCAGA TGAGACAGAA CATGGCTGAC 
CTGCAAGGCC AACAGACAAA TCCTGCACAT AGCTCAGCTG AGCTGTTGGA ACTCGTCTGT 
CCTGAGGCCA CTGGAAGAAA AACACTCTCA GGACAAGTGG GAGAAACTGG CCTTTATGTT 
GGGGGATTGA TGGAAGAATT GACTGCAAAT CCAGAGAGTA ATGGTGGACT TCCAACCCAC 
TGTGCCTCCT TATTTGATGA CTGTCCCCTC AGCAGTGCTG ACCCAATGCC CAGCTTTTGT 
TGTACAGTGG GGGTGGATTG GAAGTCTTTG ACGACACCAG CTTGTCTGCC CCTCACCACG 
GACTATTTTC CAGATCGCCA AACCCTTCAG AATGATTATA CTGAAGGCTG CTATGACCTG 
CTACCACACA GTGACCTGGA CAGGCGGGAA GAAGAAGCAA CTGTAAGAAC AGCTTCCGAA 
GTGTTTGAGG AGTTTATCTG TCAGAGACTG ATGCAGGGGT ATCAAATCAT TGTTCAGACT 
CATAACAGGA AGCCTCAGCC ATCTGTGTCC ACACCTCTTG GCAGCAGCCC TCTTTACTCC 
AGAGGCTTGG TTTCCCTGCG TCGAGCAGAA GAGAAGGAGA ATGTTTATTG GCTCAGCATG 
GGCCGTACCT TCCATAAAGT TTGCCTCAAA GACAAAATAA TCAGTGTAAC TCGCTATTTG 
CCAAAGTACC CGTGTGAGAC TGCTCAGATC CAGTACAGTT ACAGCCTTTG TCCATCACAC 
TCAGAGGGTC AATTTGTGTC CTGTTGGGTG GAGTTTGGTC ACGAACGGCT GGAAGAATAC 
AAGTGGAACT ATCTGGACCA GTACATCTGC TCTGCTGGGT CTGAGGACTT TAGTTTGATA 
GACTCTCTAA AGTTTTGGAG GACTCGGTTC TTGCTTCTGC CTGCTGGGGG AGCAAGGCGA 
ATGGCAGATG GTGAAGGGCA CTGGGATATT TATGGAGAGG CAGTGGGTAC TGCAATGGGC 
GGATCTGGGG ACTGGGTCTT GCTGGACAAC TTCATCCGCT TCCTGGAGGG CCTGAACCGC 
ATTCGTCGCC GACATCGCTC GGACAGGATC ATTAGACAAA AGGGGACACC AATGAAAGGC 
CTGCAGGTTG CTGGTACTCT CCCTTCTTAT TCTTCTGAAC ACATGGCTCC TCCTCAAGGC 
AAAAAGGGCA CATCTGCATT ATCAGTCTTA CTGGAGATGG AGCAGAAGAC ACTGGATGAA 
CAGCAGGGAA CAAAGCCCTC AACAGCTGTT GCTGACCCCT CTGGTATTGC AACAAATCTC 
ATGTATCTGG ACAGTCCTCG CAAGGATGGT GGCTTCATTT TGGAATTTAT TCGTAGCCCT 
CATTCTTCCA ACACCTATCA CTCTCAGTTG CCTGTTGAAG CCAGTGAAGC GGCGGACAGA 
GGACTCCAGC CAACAGTTGC ACCTGGAGCA GCAGTCCAGC CTGTAGGAGA GCTTGCATCT 
TGTAGCAACA CAGGAGACAC CAGTGGAAAT TCTGCAGTGC AGTCGCTTTC TTTGTCATCA 
TCCCCAACAC TCATGGAGAT CCTGGAGGCT ATAAAACATC CAATGACAGG TGTACAACTC 
CTTCCAGAGC AAAGAGGCCT GCCTCTCAAT TGCTTTTTTA GTGCGGAGGT GGTTCACTGG 
CTGATTAACA ACGTGGAAGG GGTAACGACA CAAGGCATAG CTGTAGATAT CATGCAGAAA 
ATGTTGGATG AAGGTTTGGT GGCCCATGCT TCTGGAGACA TGATGCGTAC CTTCATTTAT 
GGATTCTACT GCTACAGGAT TGTTGGAGAG AAGGATGGTT CAAATTCCCA GCCTCCTTCC 
ACAACAGCAG GGGGCTGGCC TGCAGCAGCT CTGGAGGACT TTGCCCTGTT CCAGGGAAAG 
TGGTTTGAGG TGGCCTTTGT GGTGGAAGAG CAGCTCCATT GCGATCTCCC AGTTTTTCTC 
CTGCCCTGGC TCCCCAGCAG GCCGTCCTCC TATGCAAGTA GGCATAGCTC ATTCAGCCGC 
AGTTTTGGAG GACGCAGTCA GGCCGCTGCA TTGCTAGCTG CCACTGTTCC AGAGCAGAAA 
ATGGTCACTC TGGATGTGGA TGTGAACAAT CGCAGTGACC GGATGGAGTG GTGCAGCTGT 
TATTACCATG GCAACTTCTT CCTCAACACT GCCTTTGAGA TCAAGCTTCA CTGGATGGCT 
GTCACTGCTG CAGTACTCTT TGAGATGGTT CAGGGCTGGC ACAGGAAAGC AGCATCATGT 
GGCTTCCTCT TGATCCCTGC ACTGGAGTTT CCTTTTGCAC TAACCTCCTC CCTGTATGGA 
GATCCCCTCA GAGCCCAGCA CTTCATTTCT CTGAACATCC ACTGCCTTCT AAAGAATGGC 
AGTGACAATC TGTTTGAAGG CTTTGAACCA GAGACCTACT GGGATAGGAT GCAGTTCTTT 
CAGGAGGCCA TACTTTACAG GTTTGGATTT GTGCATGACA AATTTTCAGT TTCAACCTTT 
AGTTTCCCTT CGGAGAATAA GCCCCAGTAC ATCCACATAA CAGGTACCGT CTTCTTGCAG 
CTGCCATACT TCAAGAGGAA GCATTCAAGT GGGCAGCAAC GTCGACGCCG GAACTCTACC 
ACTTCGAACA ACCAGGGGCT GTTTGGTGGG GAGGAGTGTG TTGGCTACTA TTGGGCCTAC 
AACACTATGC TGACCAAGTC CTGGAGGGCA GGCGTGCTTG GGGATGAGAA GCTCGCCGAC 
CGCCTCCTCA GAGACTTCAG CGACTTCTGT GCCAACAAAG ACAACAGACT GTTAAACTTC 
TGGGACAGCT GTCAGGAGAA AATGAACACC AGCACTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

depdc5 ( XM_029831210.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029687070.1 Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X1, mRNA.

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CloneID	OTo62424
Clone ID Related Accession (Same CDS sequence)	XM_029831210.1
Accession Version	XM_029831210.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	GATOR complex protein DEPDC5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042306.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG GTCCTGAAGA GCATAAAAGT 
GTAACATCAA ATGATAGTTT AGGCTCCGTG TCCAATGTGC TGCTCATTCC CCGTCTACCT 
TCAGCCCAGT ATGAAGTTAG CAGTTCCTTG GGATACACGA GCACCAGAGA AATGTTAGAG 
AAGATGGAGT CACAGAGGGA CTCCAGTGCA CCAGGAAGAT TTACAGTGGG GAGTGCTGAG 
TCCCCAAGGC ACATCCGTCC TGGATGCTAC ACCCCCCAAA GAGCACTCAT CAATCCTTTC 
ACCCCATCAA GGATGCCTAT GAAGCTAACC TCCAACCGCC GGCGTTGGAT GCACACCTTC 
CCCATAGGTC CCTCTGGAGA GGCAATTCAA ATCCATCATC AGATGAGACA GAACATGGCT 
GACCTGCAAG GCCAACAGAC AAATCCTGCA CATAGCTCAG CTGAGCTGTT GGAACTCGTC 
TGTCCTGAGG CCACTGGAAG AAAAACACTC TCAGGACAAG TGGGAGAAAC TGGCCTTTAT 
GTTGGGGGAT TGATGGAAGA ATTGACTGCA AATCCAGAGA GTAATGGTGG ACTTCCAACC 
CACTGTGCCT CCTTATTTGA TGACTGTCCC CTCAGCAGTG CTGACCCAAC CCTCCTGCTG 
TTTGCACCCT TGACAGTGCC CAGCTTTTGT TGTACAGTGG GGGTGGATTG GAAGTCTTTG 
ACGACACCAG CTTGTCTGCC CCTCACCACG GACTATTTTC CAGATCGCCA AACCCTTCAG 
AATGATTATA CTGAAGGCTG CTATGACCTG CTACCACACA GTGACCTGGA CAGGCGGGAA 
GAAGAAGCAA CTGTAAGAAC AGCTTCCGAA GTGTTTGAGG AGTTTATCTG TCAGAGACTG 
ATGCAGGGGT ATCAAATCAT TGTTCAGACT CATAACAGGA AGCCTCAGCC ATCTGTGTCC 
ACACCTCTTG GCAGCAGCCC TCTTTACTCC AGAGGCTTGG TTTCCCTGCG TCGAGCAGAA 
GAGAAGGAGA ATGTTTATTG GCTCAGCATG GGCCGTACCT TCCATAAAGT TTGCCTCAAA 
GACAAAATAA TCAGTGTAAC TCGCTATTTG CCAAAGTACC CGTGTGAGAC TGCTCAGATC 
CAGTACAGTT ACAGCCTTTG TCCATCACAC TCAGAGGGTC AATTTGTGTC CTGTTGGGTG 
GAGTTTGGTC ACGAACGGCT GGAAGAATAC AAGTGGAACT ATCTGGACCA GTACATCTGC 
TCTGCTGGGT CTGAGGACTT TAGTTTGATA GACTCTCTAA AGTTTTGGAG GACTCGGTTC 
TTGCTTCTGC CTGCTGGGGG AGCAAGGCGA ATGGCAGATG GTGAAGGGCA CTGGGATATT 
TATGGAGAGG CAGTGGGTAC TGCAATGGGC GGATCTGGGG ACTGGGTCTT GCTGGACAAC 
TTCATCCGCT TCCTGGAGGG CCTGAACCGC ATTCGTCGCC GACATCGCTC GGACAGGATC 
ATTAGACAAA AGGGGACACC AATGAAAGGC CTGCAGGTTG CTGGTACTCT CCCTTCTTAT 
TCTTCTGAAC ACATGGCTCC TCCTCAAGGC AAAAAGGGCA CATCTGCATT ATCAGTCTTA 
CTGGAGATGG AGCAGAAGAC ACTGGATGAA CAGCAGGGAA CAAAGCCCTC AACAGCTGTT 
GCTGACCCCT CTGGTATTGC AACAAATCTC ATGTATCTGG ACAGTCCTCG CAAGGATGGT 
GGCTTCATTT TGGAATTTAT TCGTAGCCCT CATTCTTCCA ACACCTATCA CTCTCAGTTG 
CCTGTTGAAG CCAGTGAAGC GGCGGACAGA GGACTCCAGC CAACAGTTGC ACCTGGAGCA 
GCAGTCCAGC CTGTAGGAGA GCTTGCATCT TGTAGCAACA CAGGAGACAC CAGTGGAAAT 
TCTGCAGTGC AGTCGCTTTC TTTGTCATCA TCCCCAACAC TCATGGAGAT CCTGGAGGCT 
ATAAAACATC CAATGACAGG TGTACAACTC CTTCCAGAGC AAAGAGGCCT GCCTCTCAAT 
TGCTTTTTTA GTGCGGAGGT GGTTCACTGG CTGATTAACA ACGTGGAAGG GGTAACGACA 
CAAGGCATAG CTGTAGATAT CATGCAGAAA ATGTTGGATG AAGGTTTGGT GGCCCATGCT 
TCTGGAGACA TGATGCGTAC CTTCATTTAT GGATTCTACT GCTACAGGAT TGTTGGAGAG 
AAGGATGGTT CAAATTCCCA GCCTCCTTCC ACAACAGCAG GGGGCTGGCC TGCAGCAGCT 
CTGGAGGACT TTGCCCTGTT CCAGGGAAAG TGGTTTGAGG TGGCCTTTGT GGTGGAAGAG 
CAGCTCCATT GCGATCTCCC AGTTTTTCTC CTGCCCTGGC TCCCCAGCAG GCCGTCCTCC 
TATGCAAGTA GGCATAGCTC ATTCAGCCGC AGTTTTGGAG GACGCAGTCA GGCCGCTGCA 
TTGCTAGCTG CCACTGTTCC AGAGCAGAAA ATGGTCACTC TGGATGTGGA TGTGAACAAT 
CGCAGTGACC GGATGGAGTG GTGCAGCTGT TATTACCATG GCAACTTCTT CCTCAACACT 
GCCTTTGAGA TCAAGCTTCA CTGGATGGCT GTCACTGCTG CAGTACTCTT TGAGATGGTT 
CAGGGCTGGC ACAGGAAAGC AGCATCATGT GGCTTCCTCT TGATCCCTGC ACTGGAGTTT 
CCTTTTGCAC TAACCTCCTC CCTGTATGGA GATCCCCTCA GAGCCCAGCA CTTCATTTCT 
CTGAACATCC ACTGCCTTCT AAAGAATGGC AGTGACAATC TGTTTGAAGG CTTTGAACCA 
GAGACCTACT GGGATAGGAT GCAGTTCTTT CAGGAGGCCA TACTTTACAG GTTTGGATTT 
GTGCATGACA AATTTTCAGT TTCAACCTTT AGTTTCCCTT CGGAGAATAA GCCCCAGTAC 
ATCCACATAA CAGGTACCGT CTTCTTGCAG CTGCCATACT TCAAGAGGAA GCATTCAAGT 
GGGCAGCAAC GTCGACGCCG GAACTCTACC ACTTCGAACA ACCAGGGGCT GTTTGGTGGG 
GAGGAGTGTG TTGGCTACTA TTGGGCCTAC AACACTATGC TGACCAAGTC CTGGAGGGCA 
GGCGTGCTTG GGGATGAGAA GCTCGCCGAC CGCCTCCTCA GAGACTTCAG CGACTTCTGT 
GCCAACAAAG ACAACAGACT GTTAAACTTC TGGGACAGCT GTCAGGAGAA AATGAACACC 
AGCACTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029687070.1
CDS	216..4967
Translation	MRTNKSYKLVLHKKGFGGSDDELVVNPKVFPQVCLRDIIEIAHPTDEYSPLLLQVKNLKEDLQKETISVDQTVAQAFKLRAYQDVIVNVINPKDVTLDLVELTFKDQYIGRGDMWRLKKCLVSTCAYVTQKVEFAGIRAQASELWLKGEKVTCGYISEDTRVVFRSTSAMVYIFIQMSSEMWDFDIYGDLYFEKAVNGFLSDLFAKWKEKNCSHEVTVVLFSRTFYNAKTVDEFPEVLRGSIRQDQECRFFEDFYRVVAQNERRDEWMSLLVSIKKLFVKYPLLVRLEETDGGPVGYNSTAAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRQLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRMMGVDSHVGDDYHLPHWINHSFYTSKSQTSCSSFTPRITVAGLKLTTEKSRFNKDHTLCSPKDTESSLPIQVDYDAHDIQVFRLPGPSRFKRTPNFRLGRDKEIGGRKSLGSLEVTACIGVSPPVRFEGPEEHKSVTSNDSLGSVSNVLLIPRLPSAQYEVSSSLGYTSTREMLEKMESQRDSSAPGRFTVGSAESPRHIRPGCYTPQRALINPFTPSRMPMKLTSNRRRWMHTFPIGPSGEAIQIHHQMRQNMADLQGQQTNPAHSSAELLELVCPEATGRKTLSGQVGETGLYVGGLMEELTANPESNGGLPTHCASLFDDCPLSSADPTLLLFAPLTVPSFCCTVGVDWKSLTTPACLPLTTDYFPDRQTLQNDYTEGCYDLLPHSDLDRREEEATVRTASEVFEEFICQRLMQGYQIIVQTHNRKPQPSVSTPLGSSPLYSRGLVSLRRAEEKENVYWLSMGRTFHKVCLKDKIISVTRYLPKYPCETAQIQYSYSLCPSHSEGQFVSCWVEFGHERLEEYKWNYLDQYICSAGSEDFSLIDSLKFWRTRFLLLPAGGARRMADGEGHWDIYGEAVGTAMGGSGDWVLLDNFIRFLEGLNRIRRRHRSDRIIRQKGTPMKGLQVAGTLPSYSSEHMAPPQGKKGTSALSVLLEMEQKTLDEQQGTKPSTAVADPSGIATNLMYLDSPRKDGGFILEFIRSPHSSNTYHSQLPVEASEAADRGLQPTVAPGAAVQPVGELASCSNTGDTSGNSAVQSLSLSSSPTLMEILEAIKHPMTGVQLLPEQRGLPLNCFFSAEVVHWLINNVEGVTTQGIAVDIMQKMLDEGLVAHASGDMMRTFIYGFYCYRIVGEKDGSNSQPPSTTAGGWPAAALEDFALFQGKWFEVAFVVEEQLHCDLPVFLLPWLPSRPSSYASRHSSFSRSFGGRSQAAALLAATVPEQKMVTLDVDVNNRSDRMEWCSCYYHGNFFLNTAFEIKLHWMAVTAAVLFEMVQGWHRKAASCGFLLIPALEFPFALTSSLYGDPLRAQHFISLNIHCLLKNGSDNLFEGFEPETYWDRMQFFQEAILYRFGFVHDKFSVSTFSFPSENKPQYIHITGTVFLQLPYFKRKHSSGQQRRRRNSTTSNNQGLFGGEECVGYYWAYNTMLTKSWRAGVLGDEKLADRLLRDFSDFCANKDNRLLNFWDSCQEKMNTSTP

Target ORF information:

RefSeq Version	XM_029831210.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029831210.1

ORF Insert Sequence:

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG GTCCTGAAGA GCATAAAAGT 
GTAACATCAA ATGATAGTTT AGGCTCCGTG TCCAATGTGC TGCTCATTCC CCGTCTACCT 
TCAGCCCAGT ATGAAGTTAG CAGTTCCTTG GGATACACGA GCACCAGAGA AATGTTAGAG 
AAGATGGAGT CACAGAGGGA CTCCAGTGCA CCAGGAAGAT TTACAGTGGG GAGTGCTGAG 
TCCCCAAGGC ACATCCGTCC TGGATGCTAC ACCCCCCAAA GAGCACTCAT CAATCCTTTC 
ACCCCATCAA GGATGCCTAT GAAGCTAACC TCCAACCGCC GGCGTTGGAT GCACACCTTC 
CCCATAGGTC CCTCTGGAGA GGCAATTCAA ATCCATCATC AGATGAGACA GAACATGGCT 
GACCTGCAAG GCCAACAGAC AAATCCTGCA CATAGCTCAG CTGAGCTGTT GGAACTCGTC 
TGTCCTGAGG CCACTGGAAG AAAAACACTC TCAGGACAAG TGGGAGAAAC TGGCCTTTAT 
GTTGGGGGAT TGATGGAAGA ATTGACTGCA AATCCAGAGA GTAATGGTGG ACTTCCAACC 
CACTGTGCCT CCTTATTTGA TGACTGTCCC CTCAGCAGTG CTGACCCAAC CCTCCTGCTG 
TTTGCACCCT TGACAGTGCC CAGCTTTTGT TGTACAGTGG GGGTGGATTG GAAGTCTTTG 
ACGACACCAG CTTGTCTGCC CCTCACCACG GACTATTTTC CAGATCGCCA AACCCTTCAG 
AATGATTATA CTGAAGGCTG CTATGACCTG CTACCACACA GTGACCTGGA CAGGCGGGAA 
GAAGAAGCAA CTGTAAGAAC AGCTTCCGAA GTGTTTGAGG AGTTTATCTG TCAGAGACTG 
ATGCAGGGGT ATCAAATCAT TGTTCAGACT CATAACAGGA AGCCTCAGCC ATCTGTGTCC 
ACACCTCTTG GCAGCAGCCC TCTTTACTCC AGAGGCTTGG TTTCCCTGCG TCGAGCAGAA 
GAGAAGGAGA ATGTTTATTG GCTCAGCATG GGCCGTACCT TCCATAAAGT TTGCCTCAAA 
GACAAAATAA TCAGTGTAAC TCGCTATTTG CCAAAGTACC CGTGTGAGAC TGCTCAGATC 
CAGTACAGTT ACAGCCTTTG TCCATCACAC TCAGAGGGTC AATTTGTGTC CTGTTGGGTG 
GAGTTTGGTC ACGAACGGCT GGAAGAATAC AAGTGGAACT ATCTGGACCA GTACATCTGC 
TCTGCTGGGT CTGAGGACTT TAGTTTGATA GACTCTCTAA AGTTTTGGAG GACTCGGTTC 
TTGCTTCTGC CTGCTGGGGG AGCAAGGCGA ATGGCAGATG GTGAAGGGCA CTGGGATATT 
TATGGAGAGG CAGTGGGTAC TGCAATGGGC GGATCTGGGG ACTGGGTCTT GCTGGACAAC 
TTCATCCGCT TCCTGGAGGG CCTGAACCGC ATTCGTCGCC GACATCGCTC GGACAGGATC 
ATTAGACAAA AGGGGACACC AATGAAAGGC CTGCAGGTTG CTGGTACTCT CCCTTCTTAT 
TCTTCTGAAC ACATGGCTCC TCCTCAAGGC AAAAAGGGCA CATCTGCATT ATCAGTCTTA 
CTGGAGATGG AGCAGAAGAC ACTGGATGAA CAGCAGGGAA CAAAGCCCTC AACAGCTGTT 
GCTGACCCCT CTGGTATTGC AACAAATCTC ATGTATCTGG ACAGTCCTCG CAAGGATGGT 
GGCTTCATTT TGGAATTTAT TCGTAGCCCT CATTCTTCCA ACACCTATCA CTCTCAGTTG 
CCTGTTGAAG CCAGTGAAGC GGCGGACAGA GGACTCCAGC CAACAGTTGC ACCTGGAGCA 
GCAGTCCAGC CTGTAGGAGA GCTTGCATCT TGTAGCAACA CAGGAGACAC CAGTGGAAAT 
TCTGCAGTGC AGTCGCTTTC TTTGTCATCA TCCCCAACAC TCATGGAGAT CCTGGAGGCT 
ATAAAACATC CAATGACAGG TGTACAACTC CTTCCAGAGC AAAGAGGCCT GCCTCTCAAT 
TGCTTTTTTA GTGCGGAGGT GGTTCACTGG CTGATTAACA ACGTGGAAGG GGTAACGACA 
CAAGGCATAG CTGTAGATAT CATGCAGAAA ATGTTGGATG AAGGTTTGGT GGCCCATGCT 
TCTGGAGACA TGATGCGTAC CTTCATTTAT GGATTCTACT GCTACAGGAT TGTTGGAGAG 
AAGGATGGTT CAAATTCCCA GCCTCCTTCC ACAACAGCAG GGGGCTGGCC TGCAGCAGCT 
CTGGAGGACT TTGCCCTGTT CCAGGGAAAG TGGTTTGAGG TGGCCTTTGT GGTGGAAGAG 
CAGCTCCATT GCGATCTCCC AGTTTTTCTC CTGCCCTGGC TCCCCAGCAG GCCGTCCTCC 
TATGCAAGTA GGCATAGCTC ATTCAGCCGC AGTTTTGGAG GACGCAGTCA GGCCGCTGCA 
TTGCTAGCTG CCACTGTTCC AGAGCAGAAA ATGGTCACTC TGGATGTGGA TGTGAACAAT 
CGCAGTGACC GGATGGAGTG GTGCAGCTGT TATTACCATG GCAACTTCTT CCTCAACACT 
GCCTTTGAGA TCAAGCTTCA CTGGATGGCT GTCACTGCTG CAGTACTCTT TGAGATGGTT 
CAGGGCTGGC ACAGGAAAGC AGCATCATGT GGCTTCCTCT TGATCCCTGC ACTGGAGTTT 
CCTTTTGCAC TAACCTCCTC CCTGTATGGA GATCCCCTCA GAGCCCAGCA CTTCATTTCT 
CTGAACATCC ACTGCCTTCT AAAGAATGGC AGTGACAATC TGTTTGAAGG CTTTGAACCA 
GAGACCTACT GGGATAGGAT GCAGTTCTTT CAGGAGGCCA TACTTTACAG GTTTGGATTT 
GTGCATGACA AATTTTCAGT TTCAACCTTT AGTTTCCCTT CGGAGAATAA GCCCCAGTAC 
ATCCACATAA CAGGTACCGT CTTCTTGCAG CTGCCATACT TCAAGAGGAA GCATTCAAGT 
GGGCAGCAAC GTCGACGCCG GAACTCTACC ACTTCGAACA ACCAGGGGCT GTTTGGTGGG 
GAGGAGTGTG TTGGCTACTA TTGGGCCTAC AACACTATGC TGACCAAGTC CTGGAGGGCA 
GGCGTGCTTG GGGATGAGAA GCTCGCCGAC CGCCTCCTCA GAGACTTCAG CGACTTCTGT 
GCCAACAAAG ACAACAGACT GTTAAACTTC TGGGACAGCT GTCAGGAGAA AATGAACACC 
AGCACTCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

depdc5 ( XM_029831211.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029687071.1 Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X3, mRNA.

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CloneID	OTo62425
Clone ID Related Accession (Same CDS sequence)	XM_029831211.1
Accession Version	XM_029831211.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	GATOR complex protein DEPDC5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042306.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG GTCCTGAAGA GCATAAAAGT 
GTAACATCAA ATGATAGTTT AGGCTCCGTG TCCAATGTGC TGCTCATTCC CCGTCTACCT 
TCAGCCCAGT ATGAAGTTAG CAGTTCCTTG GGATACACGA GCACCAGAGA AATGTTAGAG 
AAGATGGAGT CACAGAGGGA CTCCAGTGCA CCAGGAAGAT TTACAGTGGG GAGTGCTGAG 
TCCCCAAGGC ACATCCGTCC TGGATGCTAC ACCCCCCAAA GAGCACTCAT CAATCCTTTC 
ACCCCATCAA GGATGCCTAT GAAGCTAACC TCCAACCGCC GGCGTTGGAT GCACACCTTC 
CCCATAGGTC CCTCTGGAGA GGCAATTCAA ATCCATCATC AGATGAGACA GAACATGGCT 
GACCTGCAAG GCCAACAGAC AAATCCTGCA CATAGCTCAG CTGAGCTGTT GGAACTCGTC 
TGTCCTGAGG CCACTGGAAG AAAAACACTC TCAGGACAAG TGGGAGAAAC TGGCCTTTAT 
GTTGGGGGAT TGATGGAAGA ATTGACTGCA AATCCAGAGA GTAATGGTGG ACTTCCAACC 
CACTGTGCCT CCTTATTTGA TGACTGTCCC CTCAGCAGTG CTGACCCAAC CCTCCTGCTG 
TTTGCACCCT TGACAGTGCC CAGCTTTTGT TGTACAGTGG GGGTGGATTG GAAGTCTTTG 
ACGACACCAG CTTGTCTGCC CCTCACCACG GACTATTTTC CAGATCGCCA AACCCTTCAG 
AATGATTATA CTGAAGGCTG CTATGACCTG CTACCACACA GTGACCTGGA CAGGCGGGAA 
GAAGAAGCAA CTGTAAGAAC AGCTTCCGAA GTGTTTGAGG AGTTTATCTG TCAGAGACTG 
ATGCAGGGGT ATCAAATCAT TGTTCAGACT CATAACAGGA AGCCTCAGCC ATCTGTGTCC 
ACACCTCTTG GCAGCAGCCC TCTTTACTCC AGAGGCTTGG TTTCCCTGCG TCGAGCAGAA 
GAGAAGGAGA ATGTTTATTG GCTCAGCATG GGCCGTACCT TCCATAAAGT TTGCCTCAAA 
GACAAAATAA TCAGTGTAAC TCGCTATTTG CCAAAGTACC CGTGTGAGAC TGCTCAGATC 
CAGTACAGTT ACAGCCTTTG TCCATCACAC TCAGAGGGTC AATTTGTGTC CTGTTGGGTG 
GAGTTTGGTC ACGAACGGCT GGAAGAATAC AAGTGGAACT ATCTGGACCA GTACATCTGC 
TCTGCTGGGT CTGAGGACTT TAGTTTGATA GACTCTCTAA AGTTTTGGAG GACTCGGTTC 
TTGCTTCTGC CTGCTGGGGG AGCAAGGCGA ATGGCAGATG GTGAAGGGCA CTGGGATATT 
TATGGAGAGG CAGTGGGTAC TGCAATGGGC GGATCTGGGG ACTGGGTCTT GCTGGACAAC 
TTCATCCGCT TCCTGGAGGG CCTGAACCGC ATTCGTCGCC GACATCGCTC GGACAGGATC 
ATTAGACAAA AGGGGACACC AATGAAAGGC CTGCAGGTTG CTGGTACTCT CCCTTCTTAT 
TCTTCTGAAC ACATGGCTCC TCCTCAAGGC AAAAAGGGCA CATCTGCATT ATCAGTCTTA 
CTGGAGATGG AGCAGAAGAC ACTGGATGAA CAGCAGGGAA CAAAGCCCTC AACAGCTGTT 
GCTGACCCCT CTGGTATTGC AACAAATCTC ATGTATCTGG ACAGTCCTCG CAAGTTGCCT 
GTTGAAGCCA GTGAAGCGGC GGACAGAGGA CTCCAGCCAA CAGTTGCACC TGGAGCAGCA 
GTCCAGCCTG TAGGAGAGCT TGCATCTTGT AGCAACACAG GAGACACCAG TGGAAATTCT 
GCAGTGCAGT CGCTTTCTTT GTCATCATCC CCAACACTCA TGGAGATCCT GGAGGCTATA 
AAACATCCAA TGACAGGTGT ACAACTCCTT CCAGAGCAAA GAGGCCTGCC TCTCAATTGC 
TTTTTTAGTG CGGAGGTGGT TCACTGGCTG ATTAACAACG TGGAAGGGGT AACGACACAA 
GGCATAGCTG TAGATATCAT GCAGAAAATG TTGGATGAAG GTTTGGTGGC CCATGCTTCT 
GGAGACATGA TGCGTACCTT CATTTATGGA TTCTACTGCT ACAGGATTGT TGGAGAGAAG 
GATGGTTCAA ATTCCCAGCC TCCTTCCACA ACAGCAGGGG GCTGGCCTGC AGCAGCTCTG 
GAGGACTTTG CCCTGTTCCA GGGAAAGTGG TTTGAGGTGG CCTTTGTGGT GGAAGAGCAG 
CTCCATTGCG ATCTCCCAGT TTTTCTCCTG CCCTGGCTCC CCAGCAGGCC GTCCTCCTAT 
GCAAGTAGGC ATAGCTCATT CAGCCGCAGT TTTGGAGGAC GCAGTCAGGC CGCTGCATTG 
CTAGCTGCCA CTGTTCCAGA GCAGAAAATG GTCACTCTGG ATGTGGATGT GAACAATCGC 
AGTGACCGGA TGGAGTGGTG CAGCTGTTAT TACCATGGCA ACTTCTTCCT CAACACTGCC 
TTTGAGATCA AGCTTCACTG GATGGCTGTC ACTGCTGCAG TACTCTTTGA GATGGTTCAG 
GGCTGGCACA GGAAAGCAGC ATCATGTGGC TTCCTCTTGA TCCCTGCACT GGAGTTTCCT 
TTTGCACTAA CCTCCTCCCT GTATGGAGAT CCCCTCAGAG CCCAGCACTT CATTTCTCTG 
AACATCCACT GCCTTCTAAA GAATGGCAGT GACAATCTGT TTGAAGGCTT TGAACCAGAG 
ACCTACTGGG ATAGGATGCA GTTCTTTCAG GAGGCCATAC TTTACAGGTT TGGATTTGTG 
CATGACAAAT TTTCAGTTTC AACCTTTAGT TTCCCTTCGG AGAATAAGCC CCAGTACATC 
CACATAACAG GTACCGTCTT CTTGCAGCTG CCATACTTCA AGAGGAAGCA TTCAAGTGGG 
CAGCAACGTC GACGCCGGAA CTCTACCACT TCGAACAACC AGGGGCTGTT TGGTGGGGAG 
GAGTGTGTTG GCTACTATTG GGCCTACAAC ACTATGCTGA CCAAGTCCTG GAGGGCAGGC 
GTGCTTGGGG ATGAGAAGCT CGCCGACCGC CTCCTCAGAG ACTTCAGCGA CTTCTGTGCC 
AACAAAGACA ACAGACTGTT AAACTTCTGG GACAGCTGTC AGGAGAAAAT GAACACCAGC 
ACTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029687071.1
CDS	216..4904
Translation	MRTNKSYKLVLHKKGFGGSDDELVVNPKVFPQVCLRDIIEIAHPTDEYSPLLLQVKNLKEDLQKETISVDQTVAQAFKLRAYQDVIVNVINPKDVTLDLVELTFKDQYIGRGDMWRLKKCLVSTCAYVTQKVEFAGIRAQASELWLKGEKVTCGYISEDTRVVFRSTSAMVYIFIQMSSEMWDFDIYGDLYFEKAVNGFLSDLFAKWKEKNCSHEVTVVLFSRTFYNAKTVDEFPEVLRGSIRQDQECRFFEDFYRVVAQNERRDEWMSLLVSIKKLFVKYPLLVRLEETDGGPVGYNSTAAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRQLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRMMGVDSHVGDDYHLPHWINHSFYTSKSQTSCSSFTPRITVAGLKLTTEKSRFNKDHTLCSPKDTESSLPIQVDYDAHDIQVFRLPGPSRFKRTPNFRLGRDKEIGGRKSLGSLEVTACIGVSPPVRFEGPEEHKSVTSNDSLGSVSNVLLIPRLPSAQYEVSSSLGYTSTREMLEKMESQRDSSAPGRFTVGSAESPRHIRPGCYTPQRALINPFTPSRMPMKLTSNRRRWMHTFPIGPSGEAIQIHHQMRQNMADLQGQQTNPAHSSAELLELVCPEATGRKTLSGQVGETGLYVGGLMEELTANPESNGGLPTHCASLFDDCPLSSADPTLLLFAPLTVPSFCCTVGVDWKSLTTPACLPLTTDYFPDRQTLQNDYTEGCYDLLPHSDLDRREEEATVRTASEVFEEFICQRLMQGYQIIVQTHNRKPQPSVSTPLGSSPLYSRGLVSLRRAEEKENVYWLSMGRTFHKVCLKDKIISVTRYLPKYPCETAQIQYSYSLCPSHSEGQFVSCWVEFGHERLEEYKWNYLDQYICSAGSEDFSLIDSLKFWRTRFLLLPAGGARRMADGEGHWDIYGEAVGTAMGGSGDWVLLDNFIRFLEGLNRIRRRHRSDRIIRQKGTPMKGLQVAGTLPSYSSEHMAPPQGKKGTSALSVLLEMEQKTLDEQQGTKPSTAVADPSGIATNLMYLDSPRKLPVEASEAADRGLQPTVAPGAAVQPVGELASCSNTGDTSGNSAVQSLSLSSSPTLMEILEAIKHPMTGVQLLPEQRGLPLNCFFSAEVVHWLINNVEGVTTQGIAVDIMQKMLDEGLVAHASGDMMRTFIYGFYCYRIVGEKDGSNSQPPSTTAGGWPAAALEDFALFQGKWFEVAFVVEEQLHCDLPVFLLPWLPSRPSSYASRHSSFSRSFGGRSQAAALLAATVPEQKMVTLDVDVNNRSDRMEWCSCYYHGNFFLNTAFEIKLHWMAVTAAVLFEMVQGWHRKAASCGFLLIPALEFPFALTSSLYGDPLRAQHFISLNIHCLLKNGSDNLFEGFEPETYWDRMQFFQEAILYRFGFVHDKFSVSTFSFPSENKPQYIHITGTVFLQLPYFKRKHSSGQQRRRRNSTTSNNQGLFGGEECVGYYWAYNTMLTKSWRAGVLGDEKLADRLLRDFSDFCANKDNRLLNFWDSCQEKMNTSTP

Target ORF information:

RefSeq Version	XM_029831211.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029831211.1

ORF Insert Sequence:

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG GTCCTGAAGA GCATAAAAGT 
GTAACATCAA ATGATAGTTT AGGCTCCGTG TCCAATGTGC TGCTCATTCC CCGTCTACCT 
TCAGCCCAGT ATGAAGTTAG CAGTTCCTTG GGATACACGA GCACCAGAGA AATGTTAGAG 
AAGATGGAGT CACAGAGGGA CTCCAGTGCA CCAGGAAGAT TTACAGTGGG GAGTGCTGAG 
TCCCCAAGGC ACATCCGTCC TGGATGCTAC ACCCCCCAAA GAGCACTCAT CAATCCTTTC 
ACCCCATCAA GGATGCCTAT GAAGCTAACC TCCAACCGCC GGCGTTGGAT GCACACCTTC 
CCCATAGGTC CCTCTGGAGA GGCAATTCAA ATCCATCATC AGATGAGACA GAACATGGCT 
GACCTGCAAG GCCAACAGAC AAATCCTGCA CATAGCTCAG CTGAGCTGTT GGAACTCGTC 
TGTCCTGAGG CCACTGGAAG AAAAACACTC TCAGGACAAG TGGGAGAAAC TGGCCTTTAT 
GTTGGGGGAT TGATGGAAGA ATTGACTGCA AATCCAGAGA GTAATGGTGG ACTTCCAACC 
CACTGTGCCT CCTTATTTGA TGACTGTCCC CTCAGCAGTG CTGACCCAAC CCTCCTGCTG 
TTTGCACCCT TGACAGTGCC CAGCTTTTGT TGTACAGTGG GGGTGGATTG GAAGTCTTTG 
ACGACACCAG CTTGTCTGCC CCTCACCACG GACTATTTTC CAGATCGCCA AACCCTTCAG 
AATGATTATA CTGAAGGCTG CTATGACCTG CTACCACACA GTGACCTGGA CAGGCGGGAA 
GAAGAAGCAA CTGTAAGAAC AGCTTCCGAA GTGTTTGAGG AGTTTATCTG TCAGAGACTG 
ATGCAGGGGT ATCAAATCAT TGTTCAGACT CATAACAGGA AGCCTCAGCC ATCTGTGTCC 
ACACCTCTTG GCAGCAGCCC TCTTTACTCC AGAGGCTTGG TTTCCCTGCG TCGAGCAGAA 
GAGAAGGAGA ATGTTTATTG GCTCAGCATG GGCCGTACCT TCCATAAAGT TTGCCTCAAA 
GACAAAATAA TCAGTGTAAC TCGCTATTTG CCAAAGTACC CGTGTGAGAC TGCTCAGATC 
CAGTACAGTT ACAGCCTTTG TCCATCACAC TCAGAGGGTC AATTTGTGTC CTGTTGGGTG 
GAGTTTGGTC ACGAACGGCT GGAAGAATAC AAGTGGAACT ATCTGGACCA GTACATCTGC 
TCTGCTGGGT CTGAGGACTT TAGTTTGATA GACTCTCTAA AGTTTTGGAG GACTCGGTTC 
TTGCTTCTGC CTGCTGGGGG AGCAAGGCGA ATGGCAGATG GTGAAGGGCA CTGGGATATT 
TATGGAGAGG CAGTGGGTAC TGCAATGGGC GGATCTGGGG ACTGGGTCTT GCTGGACAAC 
TTCATCCGCT TCCTGGAGGG CCTGAACCGC ATTCGTCGCC GACATCGCTC GGACAGGATC 
ATTAGACAAA AGGGGACACC AATGAAAGGC CTGCAGGTTG CTGGTACTCT CCCTTCTTAT 
TCTTCTGAAC ACATGGCTCC TCCTCAAGGC AAAAAGGGCA CATCTGCATT ATCAGTCTTA 
CTGGAGATGG AGCAGAAGAC ACTGGATGAA CAGCAGGGAA CAAAGCCCTC AACAGCTGTT 
GCTGACCCCT CTGGTATTGC AACAAATCTC ATGTATCTGG ACAGTCCTCG CAAGTTGCCT 
GTTGAAGCCA GTGAAGCGGC GGACAGAGGA CTCCAGCCAA CAGTTGCACC TGGAGCAGCA 
GTCCAGCCTG TAGGAGAGCT TGCATCTTGT AGCAACACAG GAGACACCAG TGGAAATTCT 
GCAGTGCAGT CGCTTTCTTT GTCATCATCC CCAACACTCA TGGAGATCCT GGAGGCTATA 
AAACATCCAA TGACAGGTGT ACAACTCCTT CCAGAGCAAA GAGGCCTGCC TCTCAATTGC 
TTTTTTAGTG CGGAGGTGGT TCACTGGCTG ATTAACAACG TGGAAGGGGT AACGACACAA 
GGCATAGCTG TAGATATCAT GCAGAAAATG TTGGATGAAG GTTTGGTGGC CCATGCTTCT 
GGAGACATGA TGCGTACCTT CATTTATGGA TTCTACTGCT ACAGGATTGT TGGAGAGAAG 
GATGGTTCAA ATTCCCAGCC TCCTTCCACA ACAGCAGGGG GCTGGCCTGC AGCAGCTCTG 
GAGGACTTTG CCCTGTTCCA GGGAAAGTGG TTTGAGGTGG CCTTTGTGGT GGAAGAGCAG 
CTCCATTGCG ATCTCCCAGT TTTTCTCCTG CCCTGGCTCC CCAGCAGGCC GTCCTCCTAT 
GCAAGTAGGC ATAGCTCATT CAGCCGCAGT TTTGGAGGAC GCAGTCAGGC CGCTGCATTG 
CTAGCTGCCA CTGTTCCAGA GCAGAAAATG GTCACTCTGG ATGTGGATGT GAACAATCGC 
AGTGACCGGA TGGAGTGGTG CAGCTGTTAT TACCATGGCA ACTTCTTCCT CAACACTGCC 
TTTGAGATCA AGCTTCACTG GATGGCTGTC ACTGCTGCAG TACTCTTTGA GATGGTTCAG 
GGCTGGCACA GGAAAGCAGC ATCATGTGGC TTCCTCTTGA TCCCTGCACT GGAGTTTCCT 
TTTGCACTAA CCTCCTCCCT GTATGGAGAT CCCCTCAGAG CCCAGCACTT CATTTCTCTG 
AACATCCACT GCCTTCTAAA GAATGGCAGT GACAATCTGT TTGAAGGCTT TGAACCAGAG 
ACCTACTGGG ATAGGATGCA GTTCTTTCAG GAGGCCATAC TTTACAGGTT TGGATTTGTG 
CATGACAAAT TTTCAGTTTC AACCTTTAGT TTCCCTTCGG AGAATAAGCC CCAGTACATC 
CACATAACAG GTACCGTCTT CTTGCAGCTG CCATACTTCA AGAGGAAGCA TTCAAGTGGG 
CAGCAACGTC GACGCCGGAA CTCTACCACT TCGAACAACC AGGGGCTGTT TGGTGGGGAG 
GAGTGTGTTG GCTACTATTG GGCCTACAAC ACTATGCTGA CCAAGTCCTG GAGGGCAGGC 
GTGCTTGGGG ATGAGAAGCT CGCCGACCGC CTCCTCAGAG ACTTCAGCGA CTTCTGTGCC 
AACAAAGACA ACAGACTGTT AAACTTCTGG GACAGCTGTC AGGAGAAAAT GAACACCAGC 
ACTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

depdc5 ( XM_029831212.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029687072.1 Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X4, mRNA.

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CloneID	OTo62426
Clone ID Related Accession (Same CDS sequence)	XM_029831212.1
Accession Version	XM_029831212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	GATOR complex protein DEPDC5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042306.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG GTCCTGAAGA GCATAAAAGT 
GTAACATCAA ATGATAGTTT AGGCTCCGTG TCCAATGTGC TGCTCATTCC CCGTCTACCT 
TCAGCCCAGT ATGAAGTTAG CAGTTCCTTG GGATACACGA GCACCAGAGA AATGTTAGAG 
AAGATGGAGT CACAGAGGGA CTCCAGTGCA CCAGGAAGAT TTACAGTGGG GAGTGCTGAG 
TCCCCAAGGC ACATCCGTCC TGGATGCTAC ACCCCCCAAA GAGCACTCAT CAATCCTTTC 
ACCCCATCAA GGATGCCTAT GAAGCTAACC TCCAACCGCC GGCGTTGGAT GCACACCTTC 
CCCATAGGTC CCTCTGGAGA GGCAATTCAA ATCCATCATC AGATGAGACA GAACATGGCT 
GACCTGCAAG GCCAACAGAC AAATCCTGCA CATAGCTCAG CTGAGCTGTT GGAACTCGTC 
TGTCCTGAGG CCACTGGAAG AAAAACACTC TCAGGACAAG TGGGAGAAAC TGGCCTTTAT 
GTTGGGGGAT TGATGGAAGA ATTGACTGCA AATCCAGAGA GTAATGGTGG ACTTCCAACC 
CACTGTGCCT CCTTATTTGA TGACTGTCCC CTCAGCAGTG CTGACCCAAC CCTCCTGCTG 
TTTGCACCCT TGACAGTGCC CAGCTTTTGT TGTACAGTGG GGGTGGATTG GAAGTCTTTG 
ACGACACCAG CTTGTCTGCC CCTCACCACG GACTATTTTC CAGATCGCCA AACCCTTCAG 
AATGATTATA CTGAAGGCTG CTATGACCTG CTACCACACA GTGACCTGGA CAGGCGGGAA 
GAAGAAGCAA CTACTCATAA CAGGAAGCCT CAGCCATCTG TGTCCACACC TCTTGGCAGC 
AGCCCTCTTT ACTCCAGAGG CTTGGTTTCC CTGCGTCGAG CAGAAGAGAA GGAGAATGTT 
TATTGGCTCA GCATGGGCCG TACCTTCCAT AAAGTTTGCC TCAAAGACAA AATAATCAGT 
GTAACTCGCT ATTTGCCAAA GTACCCGTGT GAGACTGCTC AGATCCAGTA CAGTTACAGC 
CTTTGTCCAT CACACTCAGA GGGTCAATTT GTGTCCTGTT GGGTGGAGTT TGGTCACGAA 
CGGCTGGAAG AATACAAGTG GAACTATCTG GACCAGTACA TCTGCTCTGC TGGGTCTGAG 
GACTTTAGTT TGATAGACTC TCTAAAGTTT TGGAGGACTC GGTTCTTGCT TCTGCCTGCT 
GGGGGAGCAA GGCGAATGGC AGATGGTGAA GGGCACTGGG ATATTTATGG AGAGGCAGTG 
GGTACTGCAA TGGGCGGATC TGGGGACTGG GTCTTGCTGG ACAACTTCAT CCGCTTCCTG 
GAGGGCCTGA ACCGCATTCG TCGCCGACAT CGCTCGGACA GGATCATTAG ACAAAAGGGG 
ACACCAATGA AAGGCCTGCA GGTTGCTGGT ACTCTCCCTT CTTATTCTTC TGAACACATG 
GCTCCTCCTC AAGGCAAAAA GGGCACATCT GCATTATCAG TCTTACTGGA GATGGAGCAG 
AAGACACTGG ATGAACAGCA GGGAACAAAG CCCTCAACAG CTGTTGCTGA CCCCTCTGGT 
ATTGCAACAA ATCTCATGTA TCTGGACAGT CCTCGCAAGG ATGGTGGCTT CATTTTGGAA 
TTTATTCGTA GCCCTCATTC TTCCAACACC TATCACTCTC AGTTGCCTGT TGAAGCCAGT 
GAAGCGGCGG ACAGAGGACT CCAGCCAACA GTTGCACCTG GAGCAGCAGT CCAGCCTGTA 
GGAGAGCTTG CATCTTGTAG CAACACAGGA GACACCAGTG GAAATTCTGC AGTGCAGTCG 
CTTTCTTTGT CATCATCCCC AACACTCATG GAGATCCTGG AGGCTATAAA ACATCCAATG 
ACAGGTGTAC AACTCCTTCC AGAGCAAAGA GGCCTGCCTC TCAATTGCTT TTTTAGTGCG 
GAGGTGGTTC ACTGGCTGAT TAACAACGTG GAAGGGGTAA CGACACAAGG CATAGCTGTA 
GATATCATGC AGAAAATGTT GGATGAAGGT TTGGTGGCCC ATGCTTCTGG AGACATGATG 
CGTACCTTCA TTTATGGATT CTACTGCTAC AGGATTGTTG GAGAGAAGGA TGGTTCAAAT 
TCCCAGCCTC CTTCCACAAC AGCAGGGGGC TGGCCTGCAG CAGCTCTGGA GGACTTTGCC 
CTGTTCCAGG GAAAGTGGTT TGAGGTGGCC TTTGTGGTGG AAGAGCAGCT CCATTGCGAT 
CTCCCAGTTT TTCTCCTGCC CTGGCTCCCC AGCAGGCCGT CCTCCTATGC AAGTAGGCAT 
AGCTCATTCA GCCGCAGTTT TGGAGGACGC AGTCAGGCCG CTGCATTGCT AGCTGCCACT 
GTTCCAGAGC AGAAAATGGT CACTCTGGAT GTGGATGTGA ACAATCGCAG TGACCGGATG 
GAGTGGTGCA GCTGTTATTA CCATGGCAAC TTCTTCCTCA ACACTGCCTT TGAGATCAAG 
CTTCACTGGA TGGCTGTCAC TGCTGCAGTA CTCTTTGAGA TGGTTCAGGG CTGGCACAGG 
AAAGCAGCAT CATGTGGCTT CCTCTTGATC CCTGCACTGG AGTTTCCTTT TGCACTAACC 
TCCTCCCTGT ATGGAGATCC CCTCAGAGCC CAGCACTTCA TTTCTCTGAA CATCCACTGC 
CTTCTAAAGA ATGGCAGTGA CAATCTGTTT GAAGGCTTTG AACCAGAGAC CTACTGGGAT 
AGGATGCAGT TCTTTCAGGA GGCCATACTT TACAGGTTTG GATTTGTGCA TGACAAATTT 
TCAGTTTCAA CCTTTAGTTT CCCTTCGGAG AATAAGCCCC AGTACATCCA CATAACAGGT 
ACCGTCTTCT TGCAGCTGCC ATACTTCAAG AGGAAGCATT CAAGTGGGCA GCAACGTCGA 
CGCCGGAACT CTACCACTTC GAACAACCAG GGGCTGTTTG GTGGGGAGGA GTGTGTTGGC 
TACTATTGGG CCTACAACAC TATGCTGACC AAGTCCTGGA GGGCAGGCGT GCTTGGGGAT 
GAGAAGCTCG CCGACCGCCT CCTCAGAGAC TTCAGCGACT TCTGTGCCAA CAAAGACAAC 
AGACTGTTAA ACTTCTGGGA CAGCTGTCAG GAGAAAATGA ACACCAGCAC TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029687072.1
CDS	217..4893
Translation	MRTNKSYKLVLHKKGFGGSDDELVVNPKVFPQVCLRDIIEIAHPTDEYSPLLLQVKNLKEDLQKETISVDQTVAQAFKLRAYQDVIVNVINPKDVTLDLVELTFKDQYIGRGDMWRLKKCLVSTCAYVTQKVEFAGIRAQASELWLKGEKVTCGYISEDTRVVFRSTSAMVYIFIQMSSEMWDFDIYGDLYFEKAVNGFLSDLFAKWKEKNCSHEVTVVLFSRTFYNAKTVDEFPEVLRGSIRQDQECRFFEDFYRVVAQNERRDEWMSLLVSIKKLFVKYPLLVRLEETDGGPVGYNSTAAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRQLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRMMGVDSHVGDDYHLPHWINHSFYTSKSQTSCSSFTPRITVAGLKLTTEKSRFNKDHTLCSPKDTESSLPIQVDYDAHDIQVFRLPGPSRFKRTPNFRLGRDKEIGGRKSLGSLEVTACIGVSPPVRFEGPEEHKSVTSNDSLGSVSNVLLIPRLPSAQYEVSSSLGYTSTREMLEKMESQRDSSAPGRFTVGSAESPRHIRPGCYTPQRALINPFTPSRMPMKLTSNRRRWMHTFPIGPSGEAIQIHHQMRQNMADLQGQQTNPAHSSAELLELVCPEATGRKTLSGQVGETGLYVGGLMEELTANPESNGGLPTHCASLFDDCPLSSADPTLLLFAPLTVPSFCCTVGVDWKSLTTPACLPLTTDYFPDRQTLQNDYTEGCYDLLPHSDLDRREEEATTHNRKPQPSVSTPLGSSPLYSRGLVSLRRAEEKENVYWLSMGRTFHKVCLKDKIISVTRYLPKYPCETAQIQYSYSLCPSHSEGQFVSCWVEFGHERLEEYKWNYLDQYICSAGSEDFSLIDSLKFWRTRFLLLPAGGARRMADGEGHWDIYGEAVGTAMGGSGDWVLLDNFIRFLEGLNRIRRRHRSDRIIRQKGTPMKGLQVAGTLPSYSSEHMAPPQGKKGTSALSVLLEMEQKTLDEQQGTKPSTAVADPSGIATNLMYLDSPRKDGGFILEFIRSPHSSNTYHSQLPVEASEAADRGLQPTVAPGAAVQPVGELASCSNTGDTSGNSAVQSLSLSSSPTLMEILEAIKHPMTGVQLLPEQRGLPLNCFFSAEVVHWLINNVEGVTTQGIAVDIMQKMLDEGLVAHASGDMMRTFIYGFYCYRIVGEKDGSNSQPPSTTAGGWPAAALEDFALFQGKWFEVAFVVEEQLHCDLPVFLLPWLPSRPSSYASRHSSFSRSFGGRSQAAALLAATVPEQKMVTLDVDVNNRSDRMEWCSCYYHGNFFLNTAFEIKLHWMAVTAAVLFEMVQGWHRKAASCGFLLIPALEFPFALTSSLYGDPLRAQHFISLNIHCLLKNGSDNLFEGFEPETYWDRMQFFQEAILYRFGFVHDKFSVSTFSFPSENKPQYIHITGTVFLQLPYFKRKHSSGQQRRRRNSTTSNNQGLFGGEECVGYYWAYNTMLTKSWRAGVLGDEKLADRLLRDFSDFCANKDNRLLNFWDSCQEKMNTSTP

Target ORF information:

RefSeq Version	XM_029831212.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029831212.1

ORF Insert Sequence:

ATGAGAACAA ATAAGTCCTA TAAACTTGTT TTACACAAGA AAGGCTTTGG TGGCAGCGAT 
GACGAACTGG TTGTGAACCC CAAGGTTTTT CCTCAAGTAT GTCTGCGGGA TATAATTGAG 
ATAGCCCACC CCACTGATGA ATATAGCCCT CTCCTACTTC AAGTCAAAAA CCTTAAAGAA 
GACCTTCAGA AAGAGACAAT CAGTGTTGAC CAGACTGTAG CACAAGCCTT CAAACTACGG 
GCTTACCAGG ATGTCATTGT CAACGTTATC AATCCAAAGG ATGTAACACT AGACCTGGTG 
GAACTGACGT TCAAAGATCA ATACATTGGC AGAGGAGACA TGTGGAGATT AAAAAAGTGT 
TTGGTAAGCA CCTGTGCTTA TGTGACACAG AAGGTGGAAT TTGCAGGAAT CAGAGCCCAG 
GCCAGTGAAC TGTGGTTAAA GGGTGAAAAA GTGACCTGTG GGTACATCAG TGAGGACACC 
CGGGTGGTGT TTCGCTCCAC ATCTGCCATG GTTTACATCT TTATCCAGAT GAGTAGTGAG 
ATGTGGGACT TTGATATTTA TGGTGATCTC TACTTTGAGA AAGCTGTGAA TGGATTCCTC 
TCTGATCTTT TTGCCAAATG GAAGGAAAAG AATTGCAGCC ATGAGGTGAC AGTTGTTCTT 
TTCTCACGTA CATTCTACAA TGCCAAAACA GTTGATGAAT TTCCTGAAGT TCTCAGAGGG 
TCAATTAGGC AGGACCAGGA ATGCCGTTTT TTTGAAGACT TTTACAGGGT TGTTGCTCAG 
AATGAGAGGC GTGATGAGTG GATGTCATTG CTGGTCTCTA TAAAGAAGCT TTTTGTTAAG 
TATCCATTGC TGGTGCGGCT TGAAGAAACA GATGGTGGCC CGGTTGGTTA TAACTCTACT 
GCAGCTCAAG GAAACTATCT GGAAGCCATT AATCTTTCCT TCAATGTGTT TGACAAGCAT 
TACATCAACC GTAACTTTGA CCGCACTGGC CAGATGTCGG TGGTCATCAC ACCTGGGGTT 
GGAGTGTTTG AAGTTGACCG CCAGCTCATG ATTCTTACCA AACAGCGTAT GATTGATAAT 
GGTATCGGGG TGGACTTGGT GTGTATGGGG GAACAGCCTC TACATGCAGT ACCATTATTC 
AAGCTGCACA ACAGGATGAT GGGTGTGGAC TCTCATGTAG GAGATGATTA TCACCTTCCT 
CACTGGATCA ACCACAGCTT CTATACCTCC AAAAGTCAAA CCTCTTGTAG CTCCTTCACC 
CCTCGAATCA CAGTGGCTGG ACTCAAGCTC ACTACAGAGA AGTCCAGATT CAACAAGGAC 
CACACTCTCT GTTCTCCAAA GGACACTGAA AGCAGTCTGC CTATACAGGT GGACTACGAT 
GCCCATGATA TCCAGGTGTT CAGATTACCT GGTCCATCAC GATTTAAGAG GACTCCTAAT 
TTTAGGTTAG GGCGAGACAA AGAGATAGGT GGAAGAAAAA GTTTGGGCTC CCTGGAAGTG 
ACTGCATGCA TAGGGGTATC CCCCCCTGTT CGGTTTGAAG GTCCTGAAGA GCATAAAAGT 
GTAACATCAA ATGATAGTTT AGGCTCCGTG TCCAATGTGC TGCTCATTCC CCGTCTACCT 
TCAGCCCAGT ATGAAGTTAG CAGTTCCTTG GGATACACGA GCACCAGAGA AATGTTAGAG 
AAGATGGAGT CACAGAGGGA CTCCAGTGCA CCAGGAAGAT TTACAGTGGG GAGTGCTGAG 
TCCCCAAGGC ACATCCGTCC TGGATGCTAC ACCCCCCAAA GAGCACTCAT CAATCCTTTC 
ACCCCATCAA GGATGCCTAT GAAGCTAACC TCCAACCGCC GGCGTTGGAT GCACACCTTC 
CCCATAGGTC CCTCTGGAGA GGCAATTCAA ATCCATCATC AGATGAGACA GAACATGGCT 
GACCTGCAAG GCCAACAGAC AAATCCTGCA CATAGCTCAG CTGAGCTGTT GGAACTCGTC 
TGTCCTGAGG CCACTGGAAG AAAAACACTC TCAGGACAAG TGGGAGAAAC TGGCCTTTAT 
GTTGGGGGAT TGATGGAAGA ATTGACTGCA AATCCAGAGA GTAATGGTGG ACTTCCAACC 
CACTGTGCCT CCTTATTTGA TGACTGTCCC CTCAGCAGTG CTGACCCAAC CCTCCTGCTG 
TTTGCACCCT TGACAGTGCC CAGCTTTTGT TGTACAGTGG GGGTGGATTG GAAGTCTTTG 
ACGACACCAG CTTGTCTGCC CCTCACCACG GACTATTTTC CAGATCGCCA AACCCTTCAG 
AATGATTATA CTGAAGGCTG CTATGACCTG CTACCACACA GTGACCTGGA CAGGCGGGAA 
GAAGAAGCAA CTACTCATAA CAGGAAGCCT CAGCCATCTG TGTCCACACC TCTTGGCAGC 
AGCCCTCTTT ACTCCAGAGG CTTGGTTTCC CTGCGTCGAG CAGAAGAGAA GGAGAATGTT 
TATTGGCTCA GCATGGGCCG TACCTTCCAT AAAGTTTGCC TCAAAGACAA AATAATCAGT 
GTAACTCGCT ATTTGCCAAA GTACCCGTGT GAGACTGCTC AGATCCAGTA CAGTTACAGC 
CTTTGTCCAT CACACTCAGA GGGTCAATTT GTGTCCTGTT GGGTGGAGTT TGGTCACGAA 
CGGCTGGAAG AATACAAGTG GAACTATCTG GACCAGTACA TCTGCTCTGC TGGGTCTGAG 
GACTTTAGTT TGATAGACTC TCTAAAGTTT TGGAGGACTC GGTTCTTGCT TCTGCCTGCT 
GGGGGAGCAA GGCGAATGGC AGATGGTGAA GGGCACTGGG ATATTTATGG AGAGGCAGTG 
GGTACTGCAA TGGGCGGATC TGGGGACTGG GTCTTGCTGG ACAACTTCAT CCGCTTCCTG 
GAGGGCCTGA ACCGCATTCG TCGCCGACAT CGCTCGGACA GGATCATTAG ACAAAAGGGG 
ACACCAATGA AAGGCCTGCA GGTTGCTGGT ACTCTCCCTT CTTATTCTTC TGAACACATG 
GCTCCTCCTC AAGGCAAAAA GGGCACATCT GCATTATCAG TCTTACTGGA GATGGAGCAG 
AAGACACTGG ATGAACAGCA GGGAACAAAG CCCTCAACAG CTGTTGCTGA CCCCTCTGGT 
ATTGCAACAA ATCTCATGTA TCTGGACAGT CCTCGCAAGG ATGGTGGCTT CATTTTGGAA 
TTTATTCGTA GCCCTCATTC TTCCAACACC TATCACTCTC AGTTGCCTGT TGAAGCCAGT 
GAAGCGGCGG ACAGAGGACT CCAGCCAACA GTTGCACCTG GAGCAGCAGT CCAGCCTGTA 
GGAGAGCTTG CATCTTGTAG CAACACAGGA GACACCAGTG GAAATTCTGC AGTGCAGTCG 
CTTTCTTTGT CATCATCCCC AACACTCATG GAGATCCTGG AGGCTATAAA ACATCCAATG 
ACAGGTGTAC AACTCCTTCC AGAGCAAAGA GGCCTGCCTC TCAATTGCTT TTTTAGTGCG 
GAGGTGGTTC ACTGGCTGAT TAACAACGTG GAAGGGGTAA CGACACAAGG CATAGCTGTA 
GATATCATGC AGAAAATGTT GGATGAAGGT TTGGTGGCCC ATGCTTCTGG AGACATGATG 
CGTACCTTCA TTTATGGATT CTACTGCTAC AGGATTGTTG GAGAGAAGGA TGGTTCAAAT 
TCCCAGCCTC CTTCCACAAC AGCAGGGGGC TGGCCTGCAG CAGCTCTGGA GGACTTTGCC 
CTGTTCCAGG GAAAGTGGTT TGAGGTGGCC TTTGTGGTGG AAGAGCAGCT CCATTGCGAT 
CTCCCAGTTT TTCTCCTGCC CTGGCTCCCC AGCAGGCCGT CCTCCTATGC AAGTAGGCAT 
AGCTCATTCA GCCGCAGTTT TGGAGGACGC AGTCAGGCCG CTGCATTGCT AGCTGCCACT 
GTTCCAGAGC AGAAAATGGT CACTCTGGAT GTGGATGTGA ACAATCGCAG TGACCGGATG 
GAGTGGTGCA GCTGTTATTA CCATGGCAAC TTCTTCCTCA ACACTGCCTT TGAGATCAAG 
CTTCACTGGA TGGCTGTCAC TGCTGCAGTA CTCTTTGAGA TGGTTCAGGG CTGGCACAGG 
AAAGCAGCAT CATGTGGCTT CCTCTTGATC CCTGCACTGG AGTTTCCTTT TGCACTAACC 
TCCTCCCTGT ATGGAGATCC CCTCAGAGCC CAGCACTTCA TTTCTCTGAA CATCCACTGC 
CTTCTAAAGA ATGGCAGTGA CAATCTGTTT GAAGGCTTTG AACCAGAGAC CTACTGGGAT 
AGGATGCAGT TCTTTCAGGA GGCCATACTT TACAGGTTTG GATTTGTGCA TGACAAATTT 
TCAGTTTCAA CCTTTAGTTT CCCTTCGGAG AATAAGCCCC AGTACATCCA CATAACAGGT 
ACCGTCTTCT TGCAGCTGCC ATACTTCAAG AGGAAGCATT CAAGTGGGCA GCAACGTCGA 
CGCCGGAACT CTACCACTTC GAACAACCAG GGGCTGTTTG GTGGGGAGGA GTGTGTTGGC 
TACTATTGGG CCTACAACAC TATGCTGACC AAGTCCTGGA GGGCAGGCGT GCTTGGGGAT 
GAGAAGCTCG CCGACCGCCT CCTCAGAGAC TTCAGCGACT TCTGTGCCAA CAAAGACAAC 
AGACTGTTAA ACTTCTGGGA CAGCTGTCAG GAGAAAATGA ACACCAGCAC TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

depdc5 ( XM_029831213.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029687073.1 Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OTo62427
Clone ID Related Accession (Same CDS sequence)	XM_029831213.1
Accession Version	XM_029831213.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	GATOR complex protein DEPDC5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042306.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATAG CTCTCCCCCA CACTCCCCTG ATTCCCAGCC CTCTCCTACT TCAAGTCAAA 
AACCTTAAAG AAGACCTTCA GAAAGAGACA ATCAGTGTTG ACCAGACTGT AGCACAAGCC 
TTCAAACTAC GGGCTTACCA GGATGTCATT GTCAACGTTA TCAATCCAAA GGATGTAACA 
CTAGACCTGG TGGAACTGAC GTTCAAAGAT CAATACATTG GCAGAGGAGA CATGTGGAGA 
TTAAAAAAGT GTTTGGTAAG CACCTGTGCT TATGTGACAC AGAAGGTGGA ATTTGCAGGA 
ATCAGAGCCC AGGCCAGTGA ACTGTGGTTA AAGGGTGAAA AAGTGACCTG TGGGTACATC 
AGTGAGGACA CCCGGGTGGT GTTTCGCTCC ACATCTGCCA TGGTTTACAT CTTTATCCAG 
ATGAGTAGTG AGATGTGGGA CTTTGATATT TATGGTGATC TCTACTTTGA GAAAGCTGTG 
AATGGATTCC TCTCTGATCT TTTTGCCAAA TGGAAGGAAA AGAATTGCAG CCATGAGGTG 
ACAGTTGTTC TTTTCTCACG TACATTCTAC AATGCCAAAA CAGTTGATGA ATTTCCTGAA 
GTTCTCAGAG GGTCAATTAG GCAGGACCAG GAATGCCGTT TTTTTGAAGA CTTTTACAGG 
GTTGTTGCTC AGAATGAGAG GCGTGATGAG TGGATGTCAT TGCTGGTCTC TATAAAGAAG 
CTTTTTGTTA AGTATCCATT GCTGGTGCGG CTTGAAGAAA CAGATGGTGG CCCGGTTGGT 
TATAACTCTA CTGCAGCTCA AGGAAACTAT CTGGAAGCCA TTAATCTTTC CTTCAATGTG 
TTTGACAAGC ATTACATCAA CCGTAACTTT GACCGCACTG GCCAGATGTC GGTGGTCATC 
ACACCTGGGG TTGGAGTGTT TGAAGTTGAC CGCCAGCTCA TGATTCTTAC CAAACAGCGT 
ATGATTGATA ATGGTATCGG GGTGGACTTG GTGTGTATGG GGGAACAGCC TCTACATGCA 
GTACCATTAT TCAAGCTGCA CAACAGGATG ATGGGTGTGG ACTCTCATGT AGGAGATGAT 
TATCACCTTC CTCACTGGAT CAACCACAGC TTCTATACCT CCAAAAGTCA AACCTCTTGT 
AGCTCCTTCA CCCCTCGAAT CACAGTGGCT GGACTCAAGC TCACTACAGA GAAGTCCAGA 
TTCAACAAGG ACCACACTCT CTGTTCTCCA AAGGACACTG AAAGCAGTCT GCCTATACAG 
GTGGACTACG ATGCCCATGA TATCCAGGTG TTCAGATTAC CTGGTCCATC ACGATTTAAG 
AGGACTCCTA ATTTTAGGTT AGGGCGAGAC AAAGAGATAG GTGGAAGAAA AAGTTTGGGC 
TCCCTGGAAG TGACTGCATG CATAGGGGTA TCCCCCCCTG TTCGGTTTGA AGGTCCTGAA 
GAGCATAAAA GTGTAACATC AAATGATAGT TTAGGCTCCG TGTCCAATGT GCTGCTCATT 
CCCCGTCTAC CTTCAGCCCA GTATGAAGTT AGCAGTTCCT TGGGATACAC GAGCACCAGA 
GAAATGTTAG AGAAGATGGA GTCACAGAGG GACTCCAGTG CACCAGGAAG ATTTACAGTG 
GGGAGTGCTG AGTCCCCAAG GCACATCCGT CCTGGATGCT ACACCCCCCA AAGAGCACTC 
ATCAATCCTT TCACCCCATC AAGGATGCCT ATGAAGCTAA CCTCCAACCG CCGGCGTTGG 
ATGCACACCT TCCCCATAGG TCCCTCTGGA GAGGCAATTC AAATCCATCA TCAGATGAGA 
CAGAACATGG CTGACCTGCA AGGCCAACAG ACAAATCCTG CACATAGCTC AGCTGAGCTG 
TTGGAACTCG TCTGTCCTGA GGCCACTGGA AGAAAAACAC TCTCAGGACA AGTGGGAGAA 
ACTGGCCTTT ATGTTGGGGG ATTGATGGAA GAATTGACTG CAAATCCAGA GAGTAATGGT 
GGACTTCCAA CCCACTGTGC CTCCTTATTT GATGACTGTC CCCTCAGCAG TGCTGACCCA 
ACCCTCCTGC TGTTTGCACC CTTGACAGTG CCCAGCTTTT GTTGTACAGT GGGGGTGGAT 
TGGAAGTCTT TGACGACACC AGCTTGTCTG CCCCTCACCA CGGACTATTT TCCAGATCGC 
CAAACCCTTC AGAATGATTA TACTGAAGGC TGCTATGACC TGCTACCACA CAGTGACCTG 
GACAGGCGGG AAGAAGAAGC AACTGTAAGA ACAGCTTCCG AAGTGTTTGA GGAGTTTATC 
TGTCAGAGAC TGATGCAGGG GTATCAAATC ATTGTTCAGA CTCATAACAG GAAGCCTCAG 
CCATCTGTGT CCACACCTCT TGGCAGCAGC CCTCTTTACT CCAGAGGCTT GGTTTCCCTG 
CGTCGAGCAG AAGAGAAGGA GAATGTTTAT TGGCTCAGCA TGGGCCGTAC CTTCCATAAA 
GTTTGCCTCA AAGACAAAAT AATCAGTGTA ACTCGCTATT TGCCAAAGTA CCCGTGTGAG 
ACTGCTCAGA TCCAGTACAG TTACAGCCTT TGTCCATCAC ACTCAGAGGG TCAATTTGTG 
TCCTGTTGGG TGGAGTTTGG TCACGAACGG CTGGAAGAAT ACAAGTGGAA CTATCTGGAC 
CAGTACATCT GCTCTGCTGG GTCTGAGGAC TTTAGTTTGA TAGACTCTCT AAAGTTTTGG 
AGGACTCGGT TCTTGCTTCT GCCTGCTGGG GGAGCAAGGC GAATGGCAGA TGGTGAAGGG 
CACTGGGATA TTTATGGAGA GGCAGTGGGT ACTGCAATGG GCGGATCTGG GGACTGGGTC 
TTGCTGGACA ACTTCATCCG CTTCCTGGAG GGCCTGAACC GCATTCGTCG CCGACATCGC 
TCGGACAGGA TCATTAGACA AAAGGGGACA CCAATGAAAG GCCTGCAGGT TGCTGGTACT 
CTCCCTTCTT ATTCTTCTGA ACACATGGCT CCTCCTCAAG GCAAAAAGGG CACATCTGCA 
TTATCAGTCT TACTGGAGAT GGAGCAGAAG ACACTGGATG AACAGCAGGG AACAAAGCCC 
TCAACAGCTG TTGCTGACCC CTCTGGTATT GCAACAAATC TCATGTATCT GGACAGTCCT 
CGCAAGGATG GTGGCTTCAT TTTGGAATTT ATTCGTAGCC CTCATTCTTC CAACACCTAT 
CACTCTCAGT TGCCTGTTGA AGCCAGTGAA GCGGCGGACA GAGGACTCCA GCCAACAGTT 
GCACCTGGAG CAGCAGTCCA GCCTGTAGGA GAGCTTGCAT CTTGTAGCAA CACAGGAGAC 
ACCAGTGGAA ATTCTGCAGT GCAGTCGCTT TCTTTGTCAT CATCCCCAAC ACTCATGGAG 
ATCCTGGAGG CTATAAAACA TCCAATGACA GGTGTACAAC TCCTTCCAGA GCAAAGAGGC 
CTGCCTCTCA ATTGCTTTTT TAGTGCGGAG GTGGTTCACT GGCTGATTAA CAACGTGGAA 
GGGGTAACGA CACAAGGCAT AGCTGTAGAT ATCATGCAGA AAATGTTGGA TGAAGGTTTG 
GTGGCCCATG CTTCTGGAGA CATGATGCGT ACCTTCATTT ATGGATTCTA CTGCTACAGG 
ATTGTTGGAG AGAAGGATGG TTCAAATTCC CAGCCTCCTT CCACAACAGC AGGGGGCTGG 
CCTGCAGCAG CTCTGGAGGA CTTTGCCCTG TTCCAGGGAA AGTGGTTTGA GGTGGCCTTT 
GTGGTGGAAG AGCAGCTCCA TTGCGATCTC CCAGTTTTTC TCCTGCCCTG GCTCCCCAGC 
AGGCCGTCCT CCTATGCAAG TAGGCATAGC TCATTCAGCC GCAGTTTTGG AGGACGCAGT 
CAGGCCGCTG CATTGCTAGC TGCCACTGTT CCAGAGCAGA AAATGGTCAC TCTGGATGTG 
GATGTGAACA ATCGCAGTGA CCGGATGGAG TGGTGCAGCT GTTATTACCA TGGCAACTTC 
TTCCTCAACA CTGCCTTTGA GATCAAGCTT CACTGGATGG CTGTCACTGC TGCAGTACTC 
TTTGAGATGG TTCAGGGCTG GCACAGGAAA GCAGCATCAT GTGGCTTCCT CTTGATCCCT 
GCACTGGAGT TTCCTTTTGC ACTAACCTCC TCCCTGTATG GAGATCCCCT CAGAGCCCAG 
CACTTCATTT CTCTGAACAT CCACTGCCTT CTAAAGAATG GCAGTGACAA TCTGTTTGAA 
GGCTTTGAAC CAGAGACCTA CTGGGATAGG ATGCAGTTCT TTCAGGAGGC CATACTTTAC 
AGGTTTGGAT TTGTGCATGA CAAATTTTCA GTTTCAACCT TTAGTTTCCC TTCGGAGAAT 
AAGCCCCAGT ACATCCACAT AACAGGTACC GTCTTCTTGC AGCTGCCATA CTTCAAGAGG 
AAGCATTCAA GTGGGCAGCA ACGTCGACGC CGGAACTCTA CCACTTCGAA CAACCAGGGG 
CTGTTTGGTG GGGAGGAGTG TGTTGGCTAC TATTGGGCCT ACAACACTAT GCTGACCAAG 
TCCTGGAGGG CAGGCGTGCT TGGGGATGAG AAGCTCGCCG ACCGCCTCCT CAGAGACTTC 
AGCGACTTCT GTGCCAACAA AGACAACAGA CTGTTAAACT TCTGGGACAG CTGTCAGGAG 
AAAATGAACA CCAGCACTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029687073.1
CDS	16..4659
Translation	MNIALPHTPLIPSPLLLQVKNLKEDLQKETISVDQTVAQAFKLRAYQDVIVNVINPKDVTLDLVELTFKDQYIGRGDMWRLKKCLVSTCAYVTQKVEFAGIRAQASELWLKGEKVTCGYISEDTRVVFRSTSAMVYIFIQMSSEMWDFDIYGDLYFEKAVNGFLSDLFAKWKEKNCSHEVTVVLFSRTFYNAKTVDEFPEVLRGSIRQDQECRFFEDFYRVVAQNERRDEWMSLLVSIKKLFVKYPLLVRLEETDGGPVGYNSTAAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRQLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRMMGVDSHVGDDYHLPHWINHSFYTSKSQTSCSSFTPRITVAGLKLTTEKSRFNKDHTLCSPKDTESSLPIQVDYDAHDIQVFRLPGPSRFKRTPNFRLGRDKEIGGRKSLGSLEVTACIGVSPPVRFEGPEEHKSVTSNDSLGSVSNVLLIPRLPSAQYEVSSSLGYTSTREMLEKMESQRDSSAPGRFTVGSAESPRHIRPGCYTPQRALINPFTPSRMPMKLTSNRRRWMHTFPIGPSGEAIQIHHQMRQNMADLQGQQTNPAHSSAELLELVCPEATGRKTLSGQVGETGLYVGGLMEELTANPESNGGLPTHCASLFDDCPLSSADPTLLLFAPLTVPSFCCTVGVDWKSLTTPACLPLTTDYFPDRQTLQNDYTEGCYDLLPHSDLDRREEEATVRTASEVFEEFICQRLMQGYQIIVQTHNRKPQPSVSTPLGSSPLYSRGLVSLRRAEEKENVYWLSMGRTFHKVCLKDKIISVTRYLPKYPCETAQIQYSYSLCPSHSEGQFVSCWVEFGHERLEEYKWNYLDQYICSAGSEDFSLIDSLKFWRTRFLLLPAGGARRMADGEGHWDIYGEAVGTAMGGSGDWVLLDNFIRFLEGLNRIRRRHRSDRIIRQKGTPMKGLQVAGTLPSYSSEHMAPPQGKKGTSALSVLLEMEQKTLDEQQGTKPSTAVADPSGIATNLMYLDSPRKDGGFILEFIRSPHSSNTYHSQLPVEASEAADRGLQPTVAPGAAVQPVGELASCSNTGDTSGNSAVQSLSLSSSPTLMEILEAIKHPMTGVQLLPEQRGLPLNCFFSAEVVHWLINNVEGVTTQGIAVDIMQKMLDEGLVAHASGDMMRTFIYGFYCYRIVGEKDGSNSQPPSTTAGGWPAAALEDFALFQGKWFEVAFVVEEQLHCDLPVFLLPWLPSRPSSYASRHSSFSRSFGGRSQAAALLAATVPEQKMVTLDVDVNNRSDRMEWCSCYYHGNFFLNTAFEIKLHWMAVTAAVLFEMVQGWHRKAASCGFLLIPALEFPFALTSSLYGDPLRAQHFISLNIHCLLKNGSDNLFEGFEPETYWDRMQFFQEAILYRFGFVHDKFSVSTFSFPSENKPQYIHITGTVFLQLPYFKRKHSSGQQRRRRNSTTSNNQGLFGGEECVGYYWAYNTMLTKSWRAGVLGDEKLADRLLRDFSDFCANKDNRLLNFWDSCQEKMNTSTP

Target ORF information:

RefSeq Version	XM_029831213.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029831213.1

ORF Insert Sequence:

ATGAATATAG CTCTCCCCCA CACTCCCCTG ATTCCCAGCC CTCTCCTACT TCAAGTCAAA 
AACCTTAAAG AAGACCTTCA GAAAGAGACA ATCAGTGTTG ACCAGACTGT AGCACAAGCC 
TTCAAACTAC GGGCTTACCA GGATGTCATT GTCAACGTTA TCAATCCAAA GGATGTAACA 
CTAGACCTGG TGGAACTGAC GTTCAAAGAT CAATACATTG GCAGAGGAGA CATGTGGAGA 
TTAAAAAAGT GTTTGGTAAG CACCTGTGCT TATGTGACAC AGAAGGTGGA ATTTGCAGGA 
ATCAGAGCCC AGGCCAGTGA ACTGTGGTTA AAGGGTGAAA AAGTGACCTG TGGGTACATC 
AGTGAGGACA CCCGGGTGGT GTTTCGCTCC ACATCTGCCA TGGTTTACAT CTTTATCCAG 
ATGAGTAGTG AGATGTGGGA CTTTGATATT TATGGTGATC TCTACTTTGA GAAAGCTGTG 
AATGGATTCC TCTCTGATCT TTTTGCCAAA TGGAAGGAAA AGAATTGCAG CCATGAGGTG 
ACAGTTGTTC TTTTCTCACG TACATTCTAC AATGCCAAAA CAGTTGATGA ATTTCCTGAA 
GTTCTCAGAG GGTCAATTAG GCAGGACCAG GAATGCCGTT TTTTTGAAGA CTTTTACAGG 
GTTGTTGCTC AGAATGAGAG GCGTGATGAG TGGATGTCAT TGCTGGTCTC TATAAAGAAG 
CTTTTTGTTA AGTATCCATT GCTGGTGCGG CTTGAAGAAA CAGATGGTGG CCCGGTTGGT 
TATAACTCTA CTGCAGCTCA AGGAAACTAT CTGGAAGCCA TTAATCTTTC CTTCAATGTG 
TTTGACAAGC ATTACATCAA CCGTAACTTT GACCGCACTG GCCAGATGTC GGTGGTCATC 
ACACCTGGGG TTGGAGTGTT TGAAGTTGAC CGCCAGCTCA TGATTCTTAC CAAACAGCGT 
ATGATTGATA ATGGTATCGG GGTGGACTTG GTGTGTATGG GGGAACAGCC TCTACATGCA 
GTACCATTAT TCAAGCTGCA CAACAGGATG ATGGGTGTGG ACTCTCATGT AGGAGATGAT 
TATCACCTTC CTCACTGGAT CAACCACAGC TTCTATACCT CCAAAAGTCA AACCTCTTGT 
AGCTCCTTCA CCCCTCGAAT CACAGTGGCT GGACTCAAGC TCACTACAGA GAAGTCCAGA 
TTCAACAAGG ACCACACTCT CTGTTCTCCA AAGGACACTG AAAGCAGTCT GCCTATACAG 
GTGGACTACG ATGCCCATGA TATCCAGGTG TTCAGATTAC CTGGTCCATC ACGATTTAAG 
AGGACTCCTA ATTTTAGGTT AGGGCGAGAC AAAGAGATAG GTGGAAGAAA AAGTTTGGGC 
TCCCTGGAAG TGACTGCATG CATAGGGGTA TCCCCCCCTG TTCGGTTTGA AGGTCCTGAA 
GAGCATAAAA GTGTAACATC AAATGATAGT TTAGGCTCCG TGTCCAATGT GCTGCTCATT 
CCCCGTCTAC CTTCAGCCCA GTATGAAGTT AGCAGTTCCT TGGGATACAC GAGCACCAGA 
GAAATGTTAG AGAAGATGGA GTCACAGAGG GACTCCAGTG CACCAGGAAG ATTTACAGTG 
GGGAGTGCTG AGTCCCCAAG GCACATCCGT CCTGGATGCT ACACCCCCCA AAGAGCACTC 
ATCAATCCTT TCACCCCATC AAGGATGCCT ATGAAGCTAA CCTCCAACCG CCGGCGTTGG 
ATGCACACCT TCCCCATAGG TCCCTCTGGA GAGGCAATTC AAATCCATCA TCAGATGAGA 
CAGAACATGG CTGACCTGCA AGGCCAACAG ACAAATCCTG CACATAGCTC AGCTGAGCTG 
TTGGAACTCG TCTGTCCTGA GGCCACTGGA AGAAAAACAC TCTCAGGACA AGTGGGAGAA 
ACTGGCCTTT ATGTTGGGGG ATTGATGGAA GAATTGACTG CAAATCCAGA GAGTAATGGT 
GGACTTCCAA CCCACTGTGC CTCCTTATTT GATGACTGTC CCCTCAGCAG TGCTGACCCA 
ACCCTCCTGC TGTTTGCACC CTTGACAGTG CCCAGCTTTT GTTGTACAGT GGGGGTGGAT 
TGGAAGTCTT TGACGACACC AGCTTGTCTG CCCCTCACCA CGGACTATTT TCCAGATCGC 
CAAACCCTTC AGAATGATTA TACTGAAGGC TGCTATGACC TGCTACCACA CAGTGACCTG 
GACAGGCGGG AAGAAGAAGC AACTGTAAGA ACAGCTTCCG AAGTGTTTGA GGAGTTTATC 
TGTCAGAGAC TGATGCAGGG GTATCAAATC ATTGTTCAGA CTCATAACAG GAAGCCTCAG 
CCATCTGTGT CCACACCTCT TGGCAGCAGC CCTCTTTACT CCAGAGGCTT GGTTTCCCTG 
CGTCGAGCAG AAGAGAAGGA GAATGTTTAT TGGCTCAGCA TGGGCCGTAC CTTCCATAAA 
GTTTGCCTCA AAGACAAAAT AATCAGTGTA ACTCGCTATT TGCCAAAGTA CCCGTGTGAG 
ACTGCTCAGA TCCAGTACAG TTACAGCCTT TGTCCATCAC ACTCAGAGGG TCAATTTGTG 
TCCTGTTGGG TGGAGTTTGG TCACGAACGG CTGGAAGAAT ACAAGTGGAA CTATCTGGAC 
CAGTACATCT GCTCTGCTGG GTCTGAGGAC TTTAGTTTGA TAGACTCTCT AAAGTTTTGG 
AGGACTCGGT TCTTGCTTCT GCCTGCTGGG GGAGCAAGGC GAATGGCAGA TGGTGAAGGG 
CACTGGGATA TTTATGGAGA GGCAGTGGGT ACTGCAATGG GCGGATCTGG GGACTGGGTC 
TTGCTGGACA ACTTCATCCG CTTCCTGGAG GGCCTGAACC GCATTCGTCG CCGACATCGC 
TCGGACAGGA TCATTAGACA AAAGGGGACA CCAATGAAAG GCCTGCAGGT TGCTGGTACT 
CTCCCTTCTT ATTCTTCTGA ACACATGGCT CCTCCTCAAG GCAAAAAGGG CACATCTGCA 
TTATCAGTCT TACTGGAGAT GGAGCAGAAG ACACTGGATG AACAGCAGGG AACAAAGCCC 
TCAACAGCTG TTGCTGACCC CTCTGGTATT GCAACAAATC TCATGTATCT GGACAGTCCT 
CGCAAGGATG GTGGCTTCAT TTTGGAATTT ATTCGTAGCC CTCATTCTTC CAACACCTAT 
CACTCTCAGT TGCCTGTTGA AGCCAGTGAA GCGGCGGACA GAGGACTCCA GCCAACAGTT 
GCACCTGGAG CAGCAGTCCA GCCTGTAGGA GAGCTTGCAT CTTGTAGCAA CACAGGAGAC 
ACCAGTGGAA ATTCTGCAGT GCAGTCGCTT TCTTTGTCAT CATCCCCAAC ACTCATGGAG 
ATCCTGGAGG CTATAAAACA TCCAATGACA GGTGTACAAC TCCTTCCAGA GCAAAGAGGC 
CTGCCTCTCA ATTGCTTTTT TAGTGCGGAG GTGGTTCACT GGCTGATTAA CAACGTGGAA 
GGGGTAACGA CACAAGGCAT AGCTGTAGAT ATCATGCAGA AAATGTTGGA TGAAGGTTTG 
GTGGCCCATG CTTCTGGAGA CATGATGCGT ACCTTCATTT ATGGATTCTA CTGCTACAGG 
ATTGTTGGAG AGAAGGATGG TTCAAATTCC CAGCCTCCTT CCACAACAGC AGGGGGCTGG 
CCTGCAGCAG CTCTGGAGGA CTTTGCCCTG TTCCAGGGAA AGTGGTTTGA GGTGGCCTTT 
GTGGTGGAAG AGCAGCTCCA TTGCGATCTC CCAGTTTTTC TCCTGCCCTG GCTCCCCAGC 
AGGCCGTCCT CCTATGCAAG TAGGCATAGC TCATTCAGCC GCAGTTTTGG AGGACGCAGT 
CAGGCCGCTG CATTGCTAGC TGCCACTGTT CCAGAGCAGA AAATGGTCAC TCTGGATGTG 
GATGTGAACA ATCGCAGTGA CCGGATGGAG TGGTGCAGCT GTTATTACCA TGGCAACTTC 
TTCCTCAACA CTGCCTTTGA GATCAAGCTT CACTGGATGG CTGTCACTGC TGCAGTACTC 
TTTGAGATGG TTCAGGGCTG GCACAGGAAA GCAGCATCAT GTGGCTTCCT CTTGATCCCT 
GCACTGGAGT TTCCTTTTGC ACTAACCTCC TCCCTGTATG GAGATCCCCT CAGAGCCCAG 
CACTTCATTT CTCTGAACAT CCACTGCCTT CTAAAGAATG GCAGTGACAA TCTGTTTGAA 
GGCTTTGAAC CAGAGACCTA CTGGGATAGG ATGCAGTTCT TTCAGGAGGC CATACTTTAC 
AGGTTTGGAT TTGTGCATGA CAAATTTTCA GTTTCAACCT TTAGTTTCCC TTCGGAGAAT 
AAGCCCCAGT ACATCCACAT AACAGGTACC GTCTTCTTGC AGCTGCCATA CTTCAAGAGG 
AAGCATTCAA GTGGGCAGCA ACGTCGACGC CGGAACTCTA CCACTTCGAA CAACCAGGGG 
CTGTTTGGTG GGGAGGAGTG TGTTGGCTAC TATTGGGCCT ACAACACTAT GCTGACCAAG 
TCCTGGAGGG CAGGCGTGCT TGGGGATGAG AAGCTCGCCG ACCGCCTCCT CAGAGACTTC 
AGCGACTTCT GTGCCAACAA AGACAACAGA CTGTTAAACT TCTGGGACAG CTGTCAGGAG 
AAAATGAACA CCAGCACTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

depdc5 ( XM_029831214.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029687074.1 Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X6, mRNA.

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CloneID	OTo62428
Clone ID Related Accession (Same CDS sequence)	XM_029831214.1
Accession Version	XM_029831214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	GATOR complex protein DEPDC5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042306.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATAG AGACAATCAG TGTTGACCAG ACTGTAGCAC AAGCCTTCAA ACTACGGGCT 
TACCAGGATG TCATTGTCAA CGTTATCAAT CCAAAGGATG TAACACTAGA CCTGGTGGAA 
CTGACGTTCA AAGATCAATA CATTGGCAGA GGAGACATGT GGAGATTAAA AAAGTGTTTG 
GTAAGCACCT GTGCTTATGT GACACAGAAG GTGGAATTTG CAGGAATCAG AGCCCAGGCC 
AGTGAACTGT GGTTAAAGGG TGAAAAAGTG ACCTGTGGGT ACATCAGTGA GGACACCCGG 
GTGGTGTTTC GCTCCACATC TGCCATGGTT TACATCTTTA TCCAGATGAG TAGTGAGATG 
TGGGACTTTG ATATTTATGG TGATCTCTAC TTTGAGAAAG CTGTGAATGG ATTCCTCTCT 
GATCTTTTTG CCAAATGGAA GGAAAAGAAT TGCAGCCATG AGGTGACAGT TGTTCTTTTC 
TCACGTACAT TCTACAATGC CAAAACAGTT GATGAATTTC CTGAAGTTCT CAGAGGGTCA 
ATTAGGCAGG ACCAGGAATG CCGTTTTTTT GAAGACTTTT ACAGGGTTGT TGCTCAGAAT 
GAGAGGCGTG ATGAGTGGAT GTCATTGCTG GTCTCTATAA AGAAGCTTTT TGTTAAGTAT 
CCATTGCTGG TGCGGCTTGA AGAAACAGAT GGTGGCCCGG TTGGTTATAA CTCTACTGCA 
GCTCAAGGAA ACTATCTGGA AGCCATTAAT CTTTCCTTCA ATGTGTTTGA CAAGCATTAC 
ATCAACCGTA ACTTTGACCG CACTGGCCAG ATGTCGGTGG TCATCACACC TGGGGTTGGA 
GTGTTTGAAG TTGACCGCCA GCTCATGATT CTTACCAAAC AGCGTATGAT TGATAATGGT 
ATCGGGGTGG ACTTGGTGTG TATGGGGGAA CAGCCTCTAC ATGCAGTACC ATTATTCAAG 
CTGCACAACA GGATGATGGG TGTGGACTCT CATGTAGGAG ATGATTATCA CCTTCCTCAC 
TGGATCAACC ACAGCTTCTA TACCTCCAAA AGTCAAACCT CTTGTAGCTC CTTCACCCCT 
CGAATCACAG TGGCTGGACT CAAGCTCACT ACAGAGAAGT CCAGATTCAA CAAGGACCAC 
ACTCTCTGTT CTCCAAAGGA CACTGAAAGC AGTCTGCCTA TACAGGTGGA CTACGATGCC 
CATGATATCC AGGTGTTCAG ATTACCTGGT CCATCACGAT TTAAGAGGAC TCCTAATTTT 
AGGTTAGGGC GAGACAAAGA GATAGGTGGA AGAAAAAGTT TGGGCTCCCT GGAAGTGACT 
GCATGCATAG GGGTATCCCC CCCTGTTCGG TTTGAAGGTC CTGAAGAGCA TAAAAGTGTA 
ACATCAAATG ATAGTTTAGG CTCCGTGTCC AATGTGCTGC TCATTCCCCG TCTACCTTCA 
GCCCAGTATG AAGTTAGCAG TTCCTTGGGA TACACGAGCA CCAGAGAAAT GTTAGAGAAG 
ATGGAGTCAC AGAGGGACTC CAGTGCACCA GGAAGATTTA CAGTGGGGAG TGCTGAGTCC 
CCAAGGCACA TCCGTCCTGG ATGCTACACC CCCCAAAGAG CACTCATCAA TCCTTTCACC 
CCATCAAGGA TGCCTATGAA GCTAACCTCC AACCGCCGGC GTTGGATGCA CACCTTCCCC 
ATAGGTCCCT CTGGAGAGGC AATTCAAATC CATCATCAGA TGAGACAGAA CATGGCTGAC 
CTGCAAGGCC AACAGACAAA TCCTGCACAT AGCTCAGCTG AGCTGTTGGA ACTCGTCTGT 
CCTGAGGCCA CTGGAAGAAA AACACTCTCA GGACAAGTGG GAGAAACTGG CCTTTATGTT 
GGGGGATTGA TGGAAGAATT GACTGCAAAT CCAGAGAGTA ATGGTGGACT TCCAACCCAC 
TGTGCCTCCT TATTTGATGA CTGTCCCCTC AGCAGTGCTG ACCCAACCCT CCTGCTGTTT 
GCACCCTTGA CAGTGCCCAG CTTTTGTTGT ACAGTGGGGG TGGATTGGAA GTCTTTGACG 
ACACCAGCTT GTCTGCCCCT CACCACGGAC TATTTTCCAG ATCGCCAAAC CCTTCAGAAT 
GATTATACTG AAGGCTGCTA TGACCTGCTA CCACACAGTG ACCTGGACAG GCGGGAAGAA 
GAAGCAACTG TAAGAACAGC TTCCGAAGTG TTTGAGGAGT TTATCTGTCA GAGACTGATG 
CAGGGGTATC AAATCATTGT TCAGACTCAT AACAGGAAGC CTCAGCCATC TGTGTCCACA 
CCTCTTGGCA GCAGCCCTCT TTACTCCAGA GGCTTGGTTT CCCTGCGTCG AGCAGAAGAG 
AAGGAGAATG TTTATTGGCT CAGCATGGGC CGTACCTTCC ATAAAGTTTG CCTCAAAGAC 
AAAATAATCA GTGTAACTCG CTATTTGCCA AAGTACCCGT GTGAGACTGC TCAGATCCAG 
TACAGTTACA GCCTTTGTCC ATCACACTCA GAGGGTCAAT TTGTGTCCTG TTGGGTGGAG 
TTTGGTCACG AACGGCTGGA AGAATACAAG TGGAACTATC TGGACCAGTA CATCTGCTCT 
GCTGGGTCTG AGGACTTTAG TTTGATAGAC TCTCTAAAGT TTTGGAGGAC TCGGTTCTTG 
CTTCTGCCTG CTGGGGGAGC AAGGCGAATG GCAGATGGTG AAGGGCACTG GGATATTTAT 
GGAGAGGCAG TGGGTACTGC AATGGGCGGA TCTGGGGACT GGGTCTTGCT GGACAACTTC 
ATCCGCTTCC TGGAGGGCCT GAACCGCATT CGTCGCCGAC ATCGCTCGGA CAGGATCATT 
AGACAAAAGG GGACACCAAT GAAAGGCCTG CAGGTTGCTG GTACTCTCCC TTCTTATTCT 
TCTGAACACA TGGCTCCTCC TCAAGGCAAA AAGGGCACAT CTGCATTATC AGTCTTACTG 
GAGATGGAGC AGAAGACACT GGATGAACAG CAGGGAACAA AGCCCTCAAC AGCTGTTGCT 
GACCCCTCTG GTATTGCAAC AAATCTCATG TATCTGGACA GTCCTCGCAA GGATGGTGGC 
TTCATTTTGG AATTTATTCG TAGCCCTCAT TCTTCCAACA CCTATCACTC TCAGTTGCCT 
GTTGAAGCCA GTGAAGCGGC GGACAGAGGA CTCCAGCCAA CAGTTGCACC TGGAGCAGCA 
GTCCAGCCTG TAGGAGAGCT TGCATCTTGT AGCAACACAG GAGACACCAG TGGAAATTCT 
GCAGTGCAGT CGCTTTCTTT GTCATCATCC CCAACACTCA TGGAGATCCT GGAGGCTATA 
AAACATCCAA TGACAGGTGT ACAACTCCTT CCAGAGCAAA GAGGCCTGCC TCTCAATTGC 
TTTTTTAGTG CGGAGGTGGT TCACTGGCTG ATTAACAACG TGGAAGGGGT AACGACACAA 
GGCATAGCTG TAGATATCAT GCAGAAAATG TTGGATGAAG GTTTGGTGGC CCATGCTTCT 
GGAGACATGA TGCGTACCTT CATTTATGGA TTCTACTGCT ACAGGATTGT TGGAGAGAAG 
GATGGTTCAA ATTCCCAGCC TCCTTCCACA ACAGCAGGGG GCTGGCCTGC AGCAGCTCTG 
GAGGACTTTG CCCTGTTCCA GGGAAAGTGG TTTGAGGTGG CCTTTGTGGT GGAAGAGCAG 
CTCCATTGCG ATCTCCCAGT TTTTCTCCTG CCCTGGCTCC CCAGCAGGCC GTCCTCCTAT 
GCAAGTAGGC ATAGCTCATT CAGCCGCAGT TTTGGAGGAC GCAGTCAGGC CGCTGCATTG 
CTAGCTGCCA CTGTTCCAGA GCAGAAAATG GTCACTCTGG ATGTGGATGT GAACAATCGC 
AGTGACCGGA TGGAGTGGTG CAGCTGTTAT TACCATGGCA ACTTCTTCCT CAACACTGCC 
TTTGAGATCA AGCTTCACTG GATGGCTGTC ACTGCTGCAG TACTCTTTGA GATGGTTCAG 
GGCTGGCACA GGAAAGCAGC ATCATGTGGC TTCCTCTTGA TCCCTGCACT GGAGTTTCCT 
TTTGCACTAA CCTCCTCCCT GTATGGAGAT CCCCTCAGAG CCCAGCACTT CATTTCTCTG 
AACATCCACT GCCTTCTAAA GAATGGCAGT GACAATCTGT TTGAAGGCTT TGAACCAGAG 
ACCTACTGGG ATAGGATGCA GTTCTTTCAG GAGGCCATAC TTTACAGGTT TGGATTTGTG 
CATGACAAAT TTTCAGTTTC AACCTTTAGT TTCCCTTCGG AGAATAAGCC CCAGTACATC 
CACATAACAG GTACCGTCTT CTTGCAGCTG CCATACTTCA AGAGGAAGCA TTCAAGTGGG 
CAGCAACGTC GACGCCGGAA CTCTACCACT TCGAACAACC AGGGGCTGTT TGGTGGGGAG 
GAGTGTGTTG GCTACTATTG GGCCTACAAC ACTATGCTGA CCAAGTCCTG GAGGGCAGGC 
GTGCTTGGGG ATGAGAAGCT CGCCGACCGC CTCCTCAGAG ACTTCAGCGA CTTCTGTGCC 
AACAAAGACA ACAGACTGTT AAACTTCTGG GACAGCTGTC AGGAGAAAAT GAACACCAGC 
ACTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029687074.1
CDS	79..4647
Translation	MNIETISVDQTVAQAFKLRAYQDVIVNVINPKDVTLDLVELTFKDQYIGRGDMWRLKKCLVSTCAYVTQKVEFAGIRAQASELWLKGEKVTCGYISEDTRVVFRSTSAMVYIFIQMSSEMWDFDIYGDLYFEKAVNGFLSDLFAKWKEKNCSHEVTVVLFSRTFYNAKTVDEFPEVLRGSIRQDQECRFFEDFYRVVAQNERRDEWMSLLVSIKKLFVKYPLLVRLEETDGGPVGYNSTAAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRQLMILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRMMGVDSHVGDDYHLPHWINHSFYTSKSQTSCSSFTPRITVAGLKLTTEKSRFNKDHTLCSPKDTESSLPIQVDYDAHDIQVFRLPGPSRFKRTPNFRLGRDKEIGGRKSLGSLEVTACIGVSPPVRFEGPEEHKSVTSNDSLGSVSNVLLIPRLPSAQYEVSSSLGYTSTREMLEKMESQRDSSAPGRFTVGSAESPRHIRPGCYTPQRALINPFTPSRMPMKLTSNRRRWMHTFPIGPSGEAIQIHHQMRQNMADLQGQQTNPAHSSAELLELVCPEATGRKTLSGQVGETGLYVGGLMEELTANPESNGGLPTHCASLFDDCPLSSADPTLLLFAPLTVPSFCCTVGVDWKSLTTPACLPLTTDYFPDRQTLQNDYTEGCYDLLPHSDLDRREEEATVRTASEVFEEFICQRLMQGYQIIVQTHNRKPQPSVSTPLGSSPLYSRGLVSLRRAEEKENVYWLSMGRTFHKVCLKDKIISVTRYLPKYPCETAQIQYSYSLCPSHSEGQFVSCWVEFGHERLEEYKWNYLDQYICSAGSEDFSLIDSLKFWRTRFLLLPAGGARRMADGEGHWDIYGEAVGTAMGGSGDWVLLDNFIRFLEGLNRIRRRHRSDRIIRQKGTPMKGLQVAGTLPSYSSEHMAPPQGKKGTSALSVLLEMEQKTLDEQQGTKPSTAVADPSGIATNLMYLDSPRKDGGFILEFIRSPHSSNTYHSQLPVEASEAADRGLQPTVAPGAAVQPVGELASCSNTGDTSGNSAVQSLSLSSSPTLMEILEAIKHPMTGVQLLPEQRGLPLNCFFSAEVVHWLINNVEGVTTQGIAVDIMQKMLDEGLVAHASGDMMRTFIYGFYCYRIVGEKDGSNSQPPSTTAGGWPAAALEDFALFQGKWFEVAFVVEEQLHCDLPVFLLPWLPSRPSSYASRHSSFSRSFGGRSQAAALLAATVPEQKMVTLDVDVNNRSDRMEWCSCYYHGNFFLNTAFEIKLHWMAVTAAVLFEMVQGWHRKAASCGFLLIPALEFPFALTSSLYGDPLRAQHFISLNIHCLLKNGSDNLFEGFEPETYWDRMQFFQEAILYRFGFVHDKFSVSTFSFPSENKPQYIHITGTVFLQLPYFKRKHSSGQQRRRRNSTTSNNQGLFGGEECVGYYWAYNTMLTKSWRAGVLGDEKLADRLLRDFSDFCANKDNRLLNFWDSCQEKMNTSTP

Target ORF information:

RefSeq Version	XM_029831214.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes DEP domain containing 5 (depdc5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029831214.1

ORF Insert Sequence:

ATGAATATAG AGACAATCAG TGTTGACCAG ACTGTAGCAC AAGCCTTCAA ACTACGGGCT 
TACCAGGATG TCATTGTCAA CGTTATCAAT CCAAAGGATG TAACACTAGA CCTGGTGGAA 
CTGACGTTCA AAGATCAATA CATTGGCAGA GGAGACATGT GGAGATTAAA AAAGTGTTTG 
GTAAGCACCT GTGCTTATGT GACACAGAAG GTGGAATTTG CAGGAATCAG AGCCCAGGCC 
AGTGAACTGT GGTTAAAGGG TGAAAAAGTG ACCTGTGGGT ACATCAGTGA GGACACCCGG 
GTGGTGTTTC GCTCCACATC TGCCATGGTT TACATCTTTA TCCAGATGAG TAGTGAGATG 
TGGGACTTTG ATATTTATGG TGATCTCTAC TTTGAGAAAG CTGTGAATGG ATTCCTCTCT 
GATCTTTTTG CCAAATGGAA GGAAAAGAAT TGCAGCCATG AGGTGACAGT TGTTCTTTTC 
TCACGTACAT TCTACAATGC CAAAACAGTT GATGAATTTC CTGAAGTTCT CAGAGGGTCA 
ATTAGGCAGG ACCAGGAATG CCGTTTTTTT GAAGACTTTT ACAGGGTTGT TGCTCAGAAT 
GAGAGGCGTG ATGAGTGGAT GTCATTGCTG GTCTCTATAA AGAAGCTTTT TGTTAAGTAT 
CCATTGCTGG TGCGGCTTGA AGAAACAGAT GGTGGCCCGG TTGGTTATAA CTCTACTGCA 
GCTCAAGGAA ACTATCTGGA AGCCATTAAT CTTTCCTTCA ATGTGTTTGA CAAGCATTAC 
ATCAACCGTA ACTTTGACCG CACTGGCCAG ATGTCGGTGG TCATCACACC TGGGGTTGGA 
GTGTTTGAAG TTGACCGCCA GCTCATGATT CTTACCAAAC AGCGTATGAT TGATAATGGT 
ATCGGGGTGG ACTTGGTGTG TATGGGGGAA CAGCCTCTAC ATGCAGTACC ATTATTCAAG 
CTGCACAACA GGATGATGGG TGTGGACTCT CATGTAGGAG ATGATTATCA CCTTCCTCAC 
TGGATCAACC ACAGCTTCTA TACCTCCAAA AGTCAAACCT CTTGTAGCTC CTTCACCCCT 
CGAATCACAG TGGCTGGACT CAAGCTCACT ACAGAGAAGT CCAGATTCAA CAAGGACCAC 
ACTCTCTGTT CTCCAAAGGA CACTGAAAGC AGTCTGCCTA TACAGGTGGA CTACGATGCC 
CATGATATCC AGGTGTTCAG ATTACCTGGT CCATCACGAT TTAAGAGGAC TCCTAATTTT 
AGGTTAGGGC GAGACAAAGA GATAGGTGGA AGAAAAAGTT TGGGCTCCCT GGAAGTGACT 
GCATGCATAG GGGTATCCCC CCCTGTTCGG TTTGAAGGTC CTGAAGAGCA TAAAAGTGTA 
ACATCAAATG ATAGTTTAGG CTCCGTGTCC AATGTGCTGC TCATTCCCCG TCTACCTTCA 
GCCCAGTATG AAGTTAGCAG TTCCTTGGGA TACACGAGCA CCAGAGAAAT GTTAGAGAAG 
ATGGAGTCAC AGAGGGACTC CAGTGCACCA GGAAGATTTA CAGTGGGGAG TGCTGAGTCC 
CCAAGGCACA TCCGTCCTGG ATGCTACACC CCCCAAAGAG CACTCATCAA TCCTTTCACC 
CCATCAAGGA TGCCTATGAA GCTAACCTCC AACCGCCGGC GTTGGATGCA CACCTTCCCC 
ATAGGTCCCT CTGGAGAGGC AATTCAAATC CATCATCAGA TGAGACAGAA CATGGCTGAC 
CTGCAAGGCC AACAGACAAA TCCTGCACAT AGCTCAGCTG AGCTGTTGGA ACTCGTCTGT 
CCTGAGGCCA CTGGAAGAAA AACACTCTCA GGACAAGTGG GAGAAACTGG CCTTTATGTT 
GGGGGATTGA TGGAAGAATT GACTGCAAAT CCAGAGAGTA ATGGTGGACT TCCAACCCAC 
TGTGCCTCCT TATTTGATGA CTGTCCCCTC AGCAGTGCTG ACCCAACCCT CCTGCTGTTT 
GCACCCTTGA CAGTGCCCAG CTTTTGTTGT ACAGTGGGGG TGGATTGGAA GTCTTTGACG 
ACACCAGCTT GTCTGCCCCT CACCACGGAC TATTTTCCAG ATCGCCAAAC CCTTCAGAAT 
GATTATACTG AAGGCTGCTA TGACCTGCTA CCACACAGTG ACCTGGACAG GCGGGAAGAA 
GAAGCAACTG TAAGAACAGC TTCCGAAGTG TTTGAGGAGT TTATCTGTCA GAGACTGATG 
CAGGGGTATC AAATCATTGT TCAGACTCAT AACAGGAAGC CTCAGCCATC TGTGTCCACA 
CCTCTTGGCA GCAGCCCTCT TTACTCCAGA GGCTTGGTTT CCCTGCGTCG AGCAGAAGAG 
AAGGAGAATG TTTATTGGCT CAGCATGGGC CGTACCTTCC ATAAAGTTTG CCTCAAAGAC 
AAAATAATCA GTGTAACTCG CTATTTGCCA AAGTACCCGT GTGAGACTGC TCAGATCCAG 
TACAGTTACA GCCTTTGTCC ATCACACTCA GAGGGTCAAT TTGTGTCCTG TTGGGTGGAG 
TTTGGTCACG AACGGCTGGA AGAATACAAG TGGAACTATC TGGACCAGTA CATCTGCTCT 
GCTGGGTCTG AGGACTTTAG TTTGATAGAC TCTCTAAAGT TTTGGAGGAC TCGGTTCTTG 
CTTCTGCCTG CTGGGGGAGC AAGGCGAATG GCAGATGGTG AAGGGCACTG GGATATTTAT 
GGAGAGGCAG TGGGTACTGC AATGGGCGGA TCTGGGGACT GGGTCTTGCT GGACAACTTC 
ATCCGCTTCC TGGAGGGCCT GAACCGCATT CGTCGCCGAC ATCGCTCGGA CAGGATCATT 
AGACAAAAGG GGACACCAAT GAAAGGCCTG CAGGTTGCTG GTACTCTCCC TTCTTATTCT 
TCTGAACACA TGGCTCCTCC TCAAGGCAAA AAGGGCACAT CTGCATTATC AGTCTTACTG 
GAGATGGAGC AGAAGACACT GGATGAACAG CAGGGAACAA AGCCCTCAAC AGCTGTTGCT 
GACCCCTCTG GTATTGCAAC AAATCTCATG TATCTGGACA GTCCTCGCAA GGATGGTGGC 
TTCATTTTGG AATTTATTCG TAGCCCTCAT TCTTCCAACA CCTATCACTC TCAGTTGCCT 
GTTGAAGCCA GTGAAGCGGC GGACAGAGGA CTCCAGCCAA CAGTTGCACC TGGAGCAGCA 
GTCCAGCCTG TAGGAGAGCT TGCATCTTGT AGCAACACAG GAGACACCAG TGGAAATTCT 
GCAGTGCAGT CGCTTTCTTT GTCATCATCC CCAACACTCA TGGAGATCCT GGAGGCTATA 
AAACATCCAA TGACAGGTGT ACAACTCCTT CCAGAGCAAA GAGGCCTGCC TCTCAATTGC 
TTTTTTAGTG CGGAGGTGGT TCACTGGCTG ATTAACAACG TGGAAGGGGT AACGACACAA 
GGCATAGCTG TAGATATCAT GCAGAAAATG TTGGATGAAG GTTTGGTGGC CCATGCTTCT 
GGAGACATGA TGCGTACCTT CATTTATGGA TTCTACTGCT ACAGGATTGT TGGAGAGAAG 
GATGGTTCAA ATTCCCAGCC TCCTTCCACA ACAGCAGGGG GCTGGCCTGC AGCAGCTCTG 
GAGGACTTTG CCCTGTTCCA GGGAAAGTGG TTTGAGGTGG CCTTTGTGGT GGAAGAGCAG 
CTCCATTGCG ATCTCCCAGT TTTTCTCCTG CCCTGGCTCC CCAGCAGGCC GTCCTCCTAT 
GCAAGTAGGC ATAGCTCATT CAGCCGCAGT TTTGGAGGAC GCAGTCAGGC CGCTGCATTG 
CTAGCTGCCA CTGTTCCAGA GCAGAAAATG GTCACTCTGG ATGTGGATGT GAACAATCGC 
AGTGACCGGA TGGAGTGGTG CAGCTGTTAT TACCATGGCA ACTTCTTCCT CAACACTGCC 
TTTGAGATCA AGCTTCACTG GATGGCTGTC ACTGCTGCAG TACTCTTTGA GATGGTTCAG 
GGCTGGCACA GGAAAGCAGC ATCATGTGGC TTCCTCTTGA TCCCTGCACT GGAGTTTCCT 
TTTGCACTAA CCTCCTCCCT GTATGGAGAT CCCCTCAGAG CCCAGCACTT CATTTCTCTG 
AACATCCACT GCCTTCTAAA GAATGGCAGT GACAATCTGT TTGAAGGCTT TGAACCAGAG 
ACCTACTGGG ATAGGATGCA GTTCTTTCAG GAGGCCATAC TTTACAGGTT TGGATTTGTG 
CATGACAAAT TTTCAGTTTC AACCTTTAGT TTCCCTTCGG AGAATAAGCC CCAGTACATC 
CACATAACAG GTACCGTCTT CTTGCAGCTG CCATACTTCA AGAGGAAGCA TTCAAGTGGG 
CAGCAACGTC GACGCCGGAA CTCTACCACT TCGAACAACC AGGGGCTGTT TGGTGGGGAG 
GAGTGTGTTG GCTACTATTG GGCCTACAAC ACTATGCTGA CCAAGTCCTG GAGGGCAGGC 
GTGCTTGGGG ATGAGAAGCT CGCCGACCGC CTCCTCAGAG ACTTCAGCGA CTTCTGTGCC 
AACAAAGACA ACAGACTGTT AAACTTCTGG GACAGCTGTC AGGAGAAAAT GAACACCAGC 
ACTCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	depdc5
Entrez Gene ID	101064585
Full Name	DEP domain containing 5
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

depdc5 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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